131 results about "Diabetic ulcers" patented technology

The invention belongs to the technical field of polypeptides and biological medicines, and particularly relates to a WK7 series antibacterial peptide and application thereof in the aspects of resisting bacteria and preventing and treating diabetic foot, pneumonia and septicemia. According to the invention, a linear peptide WK7-line with an amino acid sequence of WKRWKRW is used as a minimum active unit, and modification is carried out to form a plurality of linear modified peptides; meanwhile, a cyclic peptide WK7-cyl is formed on the basis of the linear peptide WK7-line through cyclization, and the cyclic peptide WK7-cyl is further modified to form an annular modified peptide. The linear peptide WK7-line, the linear modified peptide, the cyclic peptide WK7-cyl and the cyclic modified peptide jointly form the WK7-series antibacterial peptide, and the WK7-series antibacterial peptide is artificially synthesized polypeptide, is small in molecular weight, easy to synthesize, free of cytotoxicity, almost free of hemolysis risk, high in sterilization speed and broad-spectrum antibacterial property and can be used for preparing the antibacterial peptide. The traditional Chinese medicine composition has a certain effect on prevention and treatment of diabetic foot ulcer, pneumonia and septicemia.

The invention discloses a COF electrostatic spinning wound dressing with near-infrared response and antioxidant activity and a preparation method of the COF electrostatic spinning wound dressing, and belongs to the field of application materials and technologies. The COFs are synthesized from cobalt hexafluorophthalocyanine (CoPcF16) and ellagic acid (EA). The COFs is prepared into the spinning membrane, and the COFs spinning membrane has very strong oxidation resistance, can effectively remove excessive endogenous ROS, can respond to near infrared to generate exogenous ROS, and can effectively kill bacteria at a wound and improve blood flow and healing of the wound. The COFs spinning membrane prepared by the invention is simple and convenient in preparation method, good in biocompatibility, strong in oxidation resistance and capable of responding to near infrared to produce ROS, and has strong application potential in the field of treatment of a series of infected wounds, especially treatment of diabetic foot ulcers.

The invention relates to substituted pyrazolo piperidine carboxylic acids, their salts and to processes for their preparation, and also to their use for preparing medicaments for the treatment and / or prophylaxis of diseases, in particular cardiovascular and cardiac diseases, preferably heart failure with reduced and preserved ejection fraction (HFrEF, HFmrEF and HFpEF), hypertension (HTN), peripheral arterial diseases (PAD, PAOD), cardio-renal and kidney diseases, preferably chronic and diabetic kidney disease (CKD and DKD), cardiopulmonary and lung diseases, preferable pulmonary hypertension (PH), and other diseases, preferably neurodegenerative diseases and different forms of dementias, fibrotic diseases, systemic sclerosis (SSc), sickle cell disease (SCD), wound healing disorders such as diabetic foot ulcer (DFU).

The invention relates to the technical field of medical dressings, and discloses a preparation method of a three-dimensional structure hydrogel dressing for diabetic foot ulcers, the hydrogel dressing is prepared from polyvinyl alcohol, polyacrylic acid, hyaluronic acid, chitosan, poly (N-isopropylacrylamide), manganese dioxide and other materials, and through formula optimization and a 3D printing technology, the three-dimensional structure hydrogel dressing for diabetic foot ulcers is prepared. High-precision structure control is realized, and the preparation requirements of hydrogel dressings in complex shapes are met; strong electrostatic interaction and hydrogen-bond interaction occur between the interface of the grid structure hydrogel and the interface of the low-viscosity colloid, so that a stable three-dimensional flexible network structure is formed; the dressing has excellent flexibility, biocompatibility, antibacterial property and a function of promoting wound healing, and can be naturally gelated and participate in treatment of a wound microenvironment.

The invention discloses bioactive gel for accelerating wound healing. The gel is prepared from the following raw materials in percentage by weight: 0.8%-1% of bomer 980, 5%-5.5% of glycerol, 15%-20% of white vaseline, 51.7%-55% of phosphate buffer, 1.8%-2% of nano royal jelly acid, 2%-3% of recombinant III type human collagen, 0.4%-0.5% of sodium hyaluronate, 1%-3% of sodium alginate, 20%-25% of polyethylene glycol-400, 1.5%-3% of dopamine-methacrylamide copolymer, 0.5%-0.8% of nano silver and 0.3%-0.5% of phenoxyethanol. The nano royal jelly acid and the nano silver have a synergistic antibacterial effect, so that the inhibition effect on drug-resistant bacteria such as MRSA and pseudomonas aeruginosa is remarkably improved, and meanwhile, the wound epithelization can be accelerated and scar formation can be reduced through the dual effects of the recombinant III type collagen and the acetylated sodium hyaluronate. The defects that a traditional dressing is narrow in antibacterial spectrum, single in repairing effect, poor in environmental adaptability and the like are overcome, and the dressing is particularly suitable for complicated wounds such as diabetic foot ulcer and second-degree burn.

The invention discloses a traditional Chinese medicine composition for treating diabetic foot ulcer and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine, the traditional Chinese medicine composition comprises the following components in parts by weight: 2-10 parts of radix saposhnikoviae, 2-10 parts of notopterygium root, 20-40 parts of ramulus mori, 10-20 parts of gallnut, 2-10 parts of cortex phellodendri, 2-10 parts of radix angelicae, 10-30 parts of rheum officinale, 2-10 parts of fructus forsythiae and 5-15 parts of honeysuckle. The traditional Chinese medicine composition has a good treatment effect on diabetic foot ulcer, and effectively solves the problem of poor curative effect in the prior art.

The invention relates to the technical field of biomarkers, in particular to a biomarker composition for diabetic foot ulcer and application of the biomarker composition. The invention provides a biomarker combination for diabetic foot ulcer. The biomarker combination comprises a DDIT4 gene and a PKM gene. According to the invention, by integrating transcriptome sequencing data and single cell sequencing data, biomarkers DDIT4 and PKM closely related to diabetic foot ulcer (DFU) are successfully screened out. The expression of the two genes in a disease group is remarkably up-regulated, and the two genes are stably expressed in different data sets, so that a reliable molecular target is provided for early diagnosis of DFU.

The invention discloses a traditional Chinese medicine composition for diabetic foot ulcer and a preparation method thereof. Comprising effective extracts of folium ginkgo, radix astragali, rhizoma corydalis and rhizoma anemones raddeanae as active pharmaceutical ingredients. The traditional Chinese medicine composition has an obvious improvement effect on diabetic foot ulcer, the cure rate of a rat with the diabetic foot ulcer can be obviously increased, wound healing is accelerated, and the action mechanism of the traditional Chinese medicine composition is closely related to a Wnt / beta-catenin signal channel. In addition, the traditional Chinese medicine composition provided by the invention also has the advantages of simplicity in preparation, low cost, convenience in carrying and remarkable effect.

The present application relates to the technical field of biological medicine, and particularly relates to a stem cell culture method for promoting wound healing and application thereof. The stem cell culture method for promoting wound healing comprises the following steps: step one, providing umbilical cord-derived mesenchymal stem cells; step two, adding luteolin and baicalin as culture medium additives in a serum-supplemented culture medium, wherein the concentration of luteolin is 1-10 micromoles / L, and the concentration of baicalin is 1-10 micromoles / L; step three, inoculating the mesenchymal stem cells into the culture medium containing the additives for culture, and the culture condition is 34-40 DEG C, 4-6% CO2, and the culture time is 48-96 hours; step four, harvesting the cultured mesenchymal stem cells to obtain stem cells for promoting wound healing. The culture method can promote the stem cells to secrete various growth factors, significantly improve the secretion of PGE2 and VEGF-C growth factors, and make the wound healing rate of the stem cell treatment group reach 96%, which has a significant effect on treating diabetic foot ulcers.

Disclosed herein is a method for treating skin wounds in a patient in need of such treatment, comprising applying a foamed composition comprising nitric oxide to the area of skin requiring such treatment. The wound may be selected from the group consisting of skin ulcers, including diabetic foot ulcers (DFUs), venous leg ulcers (VLUs), arterial ulcers, decubitus (pressure) ulcers or other pressure injuries, deep tissue injuries (DTIs), burns, abrasions, skin tears, or bruises.

The invention provides a pharmaceutical composition for treating diabetic foot ulcer as well as a preparation method and application thereof, and the pharmaceutical composition for treating diabetic foot ulcer is prepared by taking angelica sinensis, honeysuckle and scutellaria baicalensis as raw materials through pretreatment, extraction and post-treatment. The pharmaceutical composition for treating diabetic foot ulcer disclosed by the invention has a good curative effect on diabetic foot ulcer diseases; especially, an oral prescription and an external prescription are matched for use, so that the curative effect is better.

The present invention discloses the application of a conductive hydrogel combined with electrical stimulation in the treatment of diabetic foot ulcers caused by neuropathy, belonging to the field of biomedicine. The conductive hydrogel is obtained by mixing chitosan, polyvinyl alcohol, MXene and gelatin. The chitosan is chitosan modified with catechol groups, the MXene is MXene modified with polyphenols and Mg ions, and the gelatin is gelatin modified with phenylboronic acid. The condition of the electrical stimulation is 100 mV cm-1. The diabetic foot ulcers include diabetic foot ulcers caused by neuropathy. Through experimental verification, the conductive hydrogel prepared by the present invention combined with electrical stimulation can promote cell migration, accelerate the wound healing of diabetic foot ulcers and achieve functional repair. The present invention provides a new idea for the clinical treatment of diabetic foot ulcers caused by neuropathy.

The invention discloses a diabetic foot ulcer monitoring and treatment evaluation device and method based on structured light imaging. The device comprises an image acquisition module, a mechanical arm module, a man-machine interaction module and an information processing module. The mechanical arm module comprises a multi-degree-of-freedom mechanical arm, the image acquisition module comprises at least one structured light image acquisition device, and the mechanical arm drives the image acquisition module to move, acquires three-dimensional point cloud images of feet from different angles and synchronously acquires space coordinates and deflection angles of the image acquisition module; and the information processing module is used for constructing a foot three-dimensional point cloud model by utilizing the three-dimensional point cloud image and the corresponding space coordinates and deflection angles, importing the foot three-dimensional point cloud model into the trained diabetic foot detection and treatment evaluation model for analysis, and displaying a prediction result through the man-machine interaction module. According to the method, the foot three-dimensional point cloud model is reconstructed, the reconstructed model is identified, the area, the volume and the position of the wound surface are evaluated, and then the treatment effect of the current mode can be evaluated.

The invention belongs to the technical field of biomedical materials, and discloses a viola philippica exosome-loaded composite hydrogel as well as a preparation method and application thereof, the composite hydrogel is prepared by mixing methacrylated polylysine (PLMA), oxidized dextran (ODex), viola philippica exosome (V-E), a photoinitiator and a physiologically compatible solvent and performing illumination crosslinking; the preparation method comprises the following steps: respectively preparing PLMA and ODex solutions, extracting and purifying V-E, adding a photo-crosslinking agent (LAP), mixing the components to obtain a precursor liquid, and carrying out in-situ curing molding through ultraviolet-visible light irradiation. The composite hydrogel provided by the invention solves the problems that the existing wound dressing is difficult to fit with irregular wounds, is antibacterial and easy to resist drugs, and lacks active anti-inflammatory and efficient healing promotion capabilities, and is suitable for repair treatment of infectious open wounds such as burns, trauma, diabetic foot ulcer and postoperative infected incisions.

The invention discloses a targeted anti-aging microneedle drug delivery system for chronic wound repair as well as a preparation method and application of the targeted anti-aging microneedle drug delivery system. The system is composed of functionalized nanoparticles and a microneedle platform: a nano drug-loading unit which takes a polydopamine nanosphere as a carrier, entraps an anti-aging drug Bem, and modifies the surface of the nano drug-loading unit with a targeting peptide E7; according to the micro-needle delivery unit, hyaluronic acid-nitrogen carboxymethyl chitosan hydrogel is used as a matrix, a nano drug-loading unit and silver ions (Ag) are loaded, a micro-needle array is formed, and minimally invasive transdermal drug delivery is achieved. According to the invention, multiple mechanisms of anti-aging (APCs aging inhibition), antioxidation (ROS removal), antibiosis (Agaction) and immunoregulation (macrophage M2 type polarization promotion) are synergistically exerted, and the bottleneck problems of single intervention target, low drug availability and difficulty in regulating and controlling pathological microenvironment of chronic wounds (such as diabetic foot ulcer) in the traditional therapy are effectively solved.

The invention belongs to the technical field of diabetic foot clinical path management, and discloses a diabetic foot patient path management method and system based on lower limb vasculopathy grading. Mapping the ulcer position to the corresponding perfusion area and the artery segment to generate ulcer space characteristics; calculating a blood supply risk score of the perfusion area and generating a space risk map by combining the blood vessel image detection data and the microcirculation detection data; then collecting time sequence data including an ulcer index, an infection index, a blood supply index and a space risk map, and predicting the progress speed and the risk level of the future ulcer course; and finally, according to the blood supply risk score and the disease course prediction result, generating an individualized path management scheme through a joint triggering rule. According to the invention, precise and dynamic management of the diabetic foot ulcer is realized, the healing rate is improved, and the recurrence rate and the medical cost are reduced.

The invention relates to the technical field of health monitoring, in particular to a foot multi-area temperature and humidity cooperative monitoring system and method for diabetic foot ulcer risk prediction.The intelligent sensing terminal is integrated with a distributed temperature and humidity sensor array, a motion sensing unit, a microprocessor and a first wireless communication module through a flexible circuit; the mobile relay terminal is internally provided with a second wireless communication module and a terminal application program, the cloud analysis platform is deployed with a time sequence database, an artificial intelligence analysis engine and a message push service, and the medical interaction terminal provides a visual interface for medical staff and patients. According to the system and the method, real early warning is realized, the accuracy and the reliability of risk assessment are improved, a complete collaborative closed-loop system is constructed, efficient energy management and excellent user experience are realized, long-term self-optimization and self-adaptive evolution capabilities are also realized, and the clinical value of the system and the method is continuously improved.

According to various embodiments, a server for predicting determination on a diabetic foot on the basis of a plurality of pieces of data on the basis of a prediction model, comprises: a communication interface; a memory; and a processor, wherein the processor is configured to: obtain at least one piece of image data and at least one piece of first sensing data from an external server of an external user through the communication interface; obtain first ulcer output data by inputting the at least one piece of image data and the at least one piece of first sensing data to the prediction model; when it is determined that the first ulcer output data exceeds a preset first threshold value on the basis of a result of comparing the first ulcer output data with the preset first threshold value, output that a foot ulcer of the external user is likely to be diabetic foot ulcer; when it is determined that the first ulcer output data exceeds a second threshold value set to be greater than the first threshold value on the basis of a result of comparing the first ulcer output data with the second threshold value, output that the foot ulcer of the external user is highly likely to be the diabetic foot ulcer; and transmit, through the communication interface, result data for the first ulcer output data determined to exceed the first threshold value and / or the second threshold value, and guideline data presenting at least one guideline on the basis of the result data, wherein the prediction model is trained on the basis of a plurality of pieces of image data, a plurality of pieces of first sensing data, and a plurality of pieces of second sensing data, which are obtained from a plurality of users, a plurality of pieces of result data output from the plurality of users, and at least one piece of professional opinion data obtained by observing the plurality of users.

The present invention describes for the first time a method for preventing and / or reversing the phenomenon of premature senescence of skin cells and complex chronic wounds, characterized in that an effective amount of the growth hormone secretagogue peptide GHRP6 is applied. Also object of the present invention is a method for treating cases of complex chronic wounds that have developed primary or secondary refractoriness caused by the development of a cellular senescence process, transforming them from refractory wounds to responsive wounds in the presence of proliferative agents with which they unexpectedly manage to reverse and restart the healing process. Complex chronic wounds include cases of diabetic foot ulcers, venous ulcers, pressure ulcers, radiation ulcers, and other types of chronic wounds that are characterized by refractoriness to all types of treatment. These cases currently have no effective treatments.

The application discloses a traditional Chinese medicine composition for treating diabetic foot ulcers and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 2-10 parts of wind-preventing plant, 2-10 parts of Qianghuang, 20-40 parts of mulberry branch, 10-20 parts of Chinese gall, 2-10 parts of golden yellow, 2-10 parts of white peony root, 10-30 parts of rhubarb, 2-10 parts of forsythia, and 5-15 parts of honeysuckle. The traditional Chinese medicine composition has a good treatment effect on diabetic foot ulcers, and effectively solves the problem of poor curative effect in the prior art.

The present invention is a diabetic healing system for the treatment of diabetic foot ulcers. This device promotes the healing process of diabetic foot ulcers by completely offloading the pressures that an ulcer may be subjected to regardless of its size, shape or location on the foot / ankle and / or toes. The device is made of a custom rigid outer shell and a custom inner lattice structure that fully supports, stabilizes and conforms to all aspects of a patient's condition, anomalies and disfigurement. It completely offloads (zero contact) any ulceration regardless of the location on the foot, ankle or toes. The offloading occurs through the use of recessed areas that can be incorporated directly into the lattice structure, or can be included in a "plug" that fits within a small aperture in the lattice structure.

This application relates to the fields of biomedicine and wound repair technology, and particularly to the application of platelet exosomes in the treatment of diabetic wound healing. This application discloses the use of platelet exosomes in the preparation of products having one or more of the following functions: 1) prevention and / or treatment of diabetic foot; 2) promotion of wound healing and / or tissue repair; 3) shortening wound healing time; 4) promoting granulation tissue growth; 5) enhancing wound angiogenesis; 6) alleviating inflammatory responses; 7) inhibiting the expression of pro-inflammatory factors; 8) promoting the expression of anti-inflammatory factors; 9) reducing MMP-9 expression; and 10) increasing MMP-2 expression. It is particularly suitable for the treatment of diabetic foot ulcers, delayed wound healing, and related complications, and also covers the use of this product in the preparation of diabetic foot treatment drugs and medical dressings.

The application discloses a kind of auxiliary diabetic foot ulcer grading wound image analysis method, it is related to medical image analysis technical field, can solve how to based on single frame optical image automatically, accurately distinguish the problem of solid-state necrotic tissue and liquid pus exudate with visual feature highly similar in diabetic foot ulcer wound, including: determining ulcer lesion area in input image, and determine lesion equivalent diameter;Determine the first point set that all tissue sample points to be classified in ulcer lesion area constitute, and the second point set that all potential convergence positions in ulcer lesion area constitute;According to lesion equivalent diameter, first point set and second point set, construct total cost matrix;Total cost matrix is solved using preset matching algorithm, each point in first point set is classified as liquid attribute point or solid attribute point;According to the number of being classified as liquid attribute point and first quantity determine liquid component proportion, compare with preset threshold value, output diabetic foot ulcer grading result.

The present invention relates to the field of biomedical technologies, and specifically to a medicament for skin wound repair and a preparation method of its raw materials, which includes the following core steps: Select the parental species of Eisenia fetida that has been purified and propagated for 30 generations, feed L-proline chelated zinc and phenylalanine sulfonated derivatives in stages in a sterile culture medium, combine with ultraviolet light induction to activate the expression of antibacterial peptide genes, and cultivate highly active adults; Obtain a clear liquid through water bath cleaning at 40-50 °C, shearing and homogenization, 30% alcohol gradient precipitation, and multi-stage ultrafiltration; Homogenize and emulsify the raw drug with carbomer matrix and glycerol to form a gel with a particle size of 100-200 nm. The highly efficient repair effect of the present invention enables epidermal regeneration within 72 hours for severe burns and scalds; the granulation coverage rate of diabetic foot ulcers is ≥90% within 20 days; Secondly, the antibacterial activity of β-sheet conformation PrAMPs is enhanced, and the zinc chelating inducer up-regulates the gene expression level.

The present application relates to the technical field of wound dressing, and particularly relates to a chitosan modified clay-based aerogel wound dressing and a preparation method thereof. The preparation method comprises the following steps: S1: adding clay minerals into deionized water to prepare a slurry, and adding acetic acid while stirring to obtain a clay mineral slurry; S2: dissolving chitosan in an acetic acid solution to prepare a chitosan sol; S3: continuously stirring and mixing the clay mineral slurry and the chitosan sol to obtain a sol; S4: immersing the sol in a metal cation solution first, and then immersing the sol in an anion salt solution to obtain a standing product; and S5: freeze-drying the standing product to obtain a composite aerogel. The present application successfully constructs a three-dimensional porous aerogel dressing, which not only retains the strong hemostatic ability of clay, but also significantly enhances the mechanical strength and antibacterial performance, and exhibits great potential in the treatment of high-exudation complex wounds such as diabetic foot ulcers and infected wounds.

The present invention provides the application of the secretory protein CTSB (cathepsin B) in predicting the risk of vascular lesions in patients with diabetic foot ulcers (DFUs), belonging to the field of biomedicine technology. Through single-cell sequencing studies, the present invention found that the secretory protein CTSB is significantly enriched in vascular endothelial cells in the blood vessels of DFU patients, with high PPI connectivity and low expression; CTSB overexpression / knockout experiments found that CTSB is associated with the proliferation and tubulogenesis of vascular endothelial cells in human blood vessels; ELISA experiments also found that the level of secretory protein CTSB in serum can be used as a marker for predicting the risk of vascular lesions in DFU patients, and that the expression level of CTSB is negatively correlated with the Wanger grade of diabetic foot; the method can predict the risk of peripheral vascular lesions in DFU patients, has the advantages of simple operation and high sensitivity, can reduce false detection and missed detection, and has stable and reliable test results, making it suitable for large-scale detection.

The invention discloses an antibacterial dressing for diabetic foot ulcer, and belongs to the technical field of antibacterial materials, hydrogel with excellent mechanical properties is obtained by adopting dynamic covalent bonds and supramolecular action, the hydrogel has the functions of resisting bacteria, eliminating excessive ROS (reactive oxygen species) and repairing wounds, and the hydrogel has a good antibacterial effect through photo-thermal and polylysine synergistic bacteria resistance. The slow-release phenolic hydroxyl group and copper ions respectively play roles in eliminating ROS and repairing wounds.

The invention belongs to the technical field of biological medicines, and particularly relates to application of human umbilical cord mesenchymal stem cells combined with metformin in preparation of anti-DFU medicines. By constructing a diabetic foot ulcer rat model and combining in-vivo and in-vitro experiments and signal analysis, it is confirmed for the first time that hUC-MSCs and metformin have a synergistic effect in treating diabetic foot ulcer. Combined treatment is beneficial to diabetic foot ulcer wound closure, reepithelization, matrix remodeling and proliferation of blood vessels and inflammation related cells, regulation and control of vascular endothelial cells PI3K-AKT and downstream signal channels thereof can be promoted through paracrine, so that the inhibited survival, proliferation, migration and vascularization capacities of the vascular endothelial cells PI3K-AKT in a hyperglycemia environment are recovered, and the vascular endothelial cells PI3K-AKT and the downstream signal channels of the vascular endothelial cells PI3K-AKT are promoted. Further, the dynamic regulation of enhancement, maturation and weakening of new angiogenesis of the wound of the DFU rat is promoted, and the wound healing is accelerated. The invention provides theoretical basis and experimental support for developing a more effective treatment scheme.

To provide the use of immunoregulatory macrophages for treating microvascular or macrovascular disorders of the lower extremities, such as diabetic foot ulcers or venous leg ulcers.SOLUTION: An immunoregulatory macrophage expressing the markers CD258, DHRS9 and IDO for use in a method of treating a microvascular or macrovascular disorder of the lower extremities in a subject is provided. Also provided is a pharmaceutical composition for use in a method of treating a microvascular or macrovascular disorder of the lower limb in a subject comprising said macrophages.SELECTED DRAWING: None