71 results about "Diabetic ulcers" patented technology

The invention belongs to the technical field of polypeptides and biological medicines, and particularly relates to a WK7 series antibacterial peptide and application thereof in the aspects of resisting bacteria and preventing and treating diabetic foot, pneumonia and septicemia. According to the invention, a linear peptide WK7-line with an amino acid sequence of WKRWKRW is used as a minimum active unit, and modification is carried out to form a plurality of linear modified peptides; meanwhile, a cyclic peptide WK7-cyl is formed on the basis of the linear peptide WK7-line through cyclization, and the cyclic peptide WK7-cyl is further modified to form an annular modified peptide. The linear peptide WK7-line, the linear modified peptide, the cyclic peptide WK7-cyl and the cyclic modified peptide jointly form the WK7-series antibacterial peptide, and the WK7-series antibacterial peptide is artificially synthesized polypeptide, is small in molecular weight, easy to synthesize, free of cytotoxicity, almost free of hemolysis risk, high in sterilization speed and broad-spectrum antibacterial property and can be used for preparing the antibacterial peptide. The traditional Chinese medicine composition has a certain effect on prevention and treatment of diabetic foot ulcer, pneumonia and septicemia.

The invention relates to substituted pyrazolo piperidine carboxylic acids, their salts and to processes for their preparation, and also to their use for preparing medicaments for the treatment and / or prophylaxis of diseases, in particular cardiovascular and cardiac diseases, preferably heart failure with reduced and preserved ejection fraction (HFrEF, HFmrEF and HFpEF), hypertension (HTN), peripheral arterial diseases (PAD, PAOD), cardio-renal and kidney diseases, preferably chronic and diabetic kidney disease (CKD and DKD), cardiopulmonary and lung diseases, preferable pulmonary hypertension (PH), and other diseases, preferably neurodegenerative diseases and different forms of dementias, fibrotic diseases, systemic sclerosis (SSc), sickle cell disease (SCD), wound healing disorders such as diabetic foot ulcer (DFU).

The invention relates to the technical field of medical dressings, and discloses a preparation method of a three-dimensional structure hydrogel dressing for diabetic foot ulcers, the hydrogel dressing is prepared from polyvinyl alcohol, polyacrylic acid, hyaluronic acid, chitosan, poly (N-isopropylacrylamide), manganese dioxide and other materials, and through formula optimization and a 3D printing technology, the three-dimensional structure hydrogel dressing for diabetic foot ulcers is prepared. High-precision structure control is realized, and the preparation requirements of hydrogel dressings in complex shapes are met; strong electrostatic interaction and hydrogen-bond interaction occur between the interface of the grid structure hydrogel and the interface of the low-viscosity colloid, so that a stable three-dimensional flexible network structure is formed; the dressing has excellent flexibility, biocompatibility, antibacterial property and a function of promoting wound healing, and can be naturally gelated and participate in treatment of a wound microenvironment.

The invention discloses a diabetic foot ulcer monitoring and treatment evaluation device and method based on structured light imaging. The device comprises an image acquisition module, a mechanical arm module, a man-machine interaction module and an information processing module. The mechanical arm module comprises a multi-degree-of-freedom mechanical arm, the image acquisition module comprises at least one structured light image acquisition device, and the mechanical arm drives the image acquisition module to move, acquires three-dimensional point cloud images of feet from different angles and synchronously acquires space coordinates and deflection angles of the image acquisition module; and the information processing module is used for constructing a foot three-dimensional point cloud model by utilizing the three-dimensional point cloud image and the corresponding space coordinates and deflection angles, importing the foot three-dimensional point cloud model into the trained diabetic foot detection and treatment evaluation model for analysis, and displaying a prediction result through the man-machine interaction module. According to the method, the foot three-dimensional point cloud model is reconstructed, the reconstructed model is identified, the area, the volume and the position of the wound surface are evaluated, and then the treatment effect of the current mode can be evaluated.

The invention belongs to the technical field of biomedical materials, and discloses a viola philippica exosome-loaded composite hydrogel as well as a preparation method and application thereof, the composite hydrogel is prepared by mixing methacrylated polylysine (PLMA), oxidized dextran (ODex), viola philippica exosome (V-E), a photoinitiator and a physiologically compatible solvent and performing illumination crosslinking; the preparation method comprises the following steps: respectively preparing PLMA and ODex solutions, extracting and purifying V-E, adding a photo-crosslinking agent (LAP), mixing the components to obtain a precursor liquid, and carrying out in-situ curing molding through ultraviolet-visible light irradiation. The composite hydrogel provided by the invention solves the problems that the existing wound dressing is difficult to fit with irregular wounds, is antibacterial and easy to resist drugs, and lacks active anti-inflammatory and efficient healing promotion capabilities, and is suitable for repair treatment of infectious open wounds such as burns, trauma, diabetic foot ulcer and postoperative infected incisions.

The invention discloses a targeted anti-aging microneedle drug delivery system for chronic wound repair as well as a preparation method and application of the targeted anti-aging microneedle drug delivery system. The system is composed of functionalized nanoparticles and a microneedle platform: a nano drug-loading unit which takes a polydopamine nanosphere as a carrier, entraps an anti-aging drug Bem, and modifies the surface of the nano drug-loading unit with a targeting peptide E7; according to the micro-needle delivery unit, hyaluronic acid-nitrogen carboxymethyl chitosan hydrogel is used as a matrix, a nano drug-loading unit and silver ions (Ag) are loaded, a micro-needle array is formed, and minimally invasive transdermal drug delivery is achieved. According to the invention, multiple mechanisms of anti-aging (APCs aging inhibition), antioxidation (ROS removal), antibiosis (Agaction) and immunoregulation (macrophage M2 type polarization promotion) are synergistically exerted, and the bottleneck problems of single intervention target, low drug availability and difficulty in regulating and controlling pathological microenvironment of chronic wounds (such as diabetic foot ulcer) in the traditional therapy are effectively solved.

The invention belongs to the technical field of diabetic foot clinical path management, and discloses a diabetic foot patient path management method and system based on lower limb vasculopathy grading. Mapping the ulcer position to the corresponding perfusion area and the artery segment to generate ulcer space characteristics; calculating a blood supply risk score of the perfusion area and generating a space risk map by combining the blood vessel image detection data and the microcirculation detection data; then collecting time sequence data including an ulcer index, an infection index, a blood supply index and a space risk map, and predicting the progress speed and the risk level of the future ulcer course; and finally, according to the blood supply risk score and the disease course prediction result, generating an individualized path management scheme through a joint triggering rule. According to the invention, precise and dynamic management of the diabetic foot ulcer is realized, the healing rate is improved, and the recurrence rate and the medical cost are reduced.

The invention relates to the technical field of health monitoring, in particular to a foot multi-area temperature and humidity cooperative monitoring system and method for diabetic foot ulcer risk prediction.The intelligent sensing terminal is integrated with a distributed temperature and humidity sensor array, a motion sensing unit, a microprocessor and a first wireless communication module through a flexible circuit; the mobile relay terminal is internally provided with a second wireless communication module and a terminal application program, the cloud analysis platform is deployed with a time sequence database, an artificial intelligence analysis engine and a message push service, and the medical interaction terminal provides a visual interface for medical staff and patients. According to the system and the method, real early warning is realized, the accuracy and the reliability of risk assessment are improved, a complete collaborative closed-loop system is constructed, efficient energy management and excellent user experience are realized, long-term self-optimization and self-adaptive evolution capabilities are also realized, and the clinical value of the system and the method is continuously improved.

The application discloses a traditional Chinese medicine composition for treating diabetic foot ulcers and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 2-10 parts of wind-preventing plant, 2-10 parts of Qianghuang, 20-40 parts of mulberry branch, 10-20 parts of Chinese gall, 2-10 parts of golden yellow, 2-10 parts of white peony root, 10-30 parts of rhubarb, 2-10 parts of forsythia, and 5-15 parts of honeysuckle. The traditional Chinese medicine composition has a good treatment effect on diabetic foot ulcers, and effectively solves the problem of poor curative effect in the prior art.

This application relates to the fields of biomedicine and wound repair technology, and particularly to the application of platelet exosomes in the treatment of diabetic wound healing. This application discloses the use of platelet exosomes in the preparation of products having one or more of the following functions: 1) prevention and / or treatment of diabetic foot; 2) promotion of wound healing and / or tissue repair; 3) shortening wound healing time; 4) promoting granulation tissue growth; 5) enhancing wound angiogenesis; 6) alleviating inflammatory responses; 7) inhibiting the expression of pro-inflammatory factors; 8) promoting the expression of anti-inflammatory factors; 9) reducing MMP-9 expression; and 10) increasing MMP-2 expression. It is particularly suitable for the treatment of diabetic foot ulcers, delayed wound healing, and related complications, and also covers the use of this product in the preparation of diabetic foot treatment drugs and medical dressings.

The application discloses a kind of auxiliary diabetic foot ulcer grading wound image analysis method, it is related to medical image analysis technical field, can solve how to based on single frame optical image automatically, accurately distinguish the problem of solid-state necrotic tissue and liquid pus exudate with visual feature highly similar in diabetic foot ulcer wound, including: determining ulcer lesion area in input image, and determine lesion equivalent diameter;Determine the first point set that all tissue sample points to be classified in ulcer lesion area constitute, and the second point set that all potential convergence positions in ulcer lesion area constitute;According to lesion equivalent diameter, first point set and second point set, construct total cost matrix;Total cost matrix is solved using preset matching algorithm, each point in first point set is classified as liquid attribute point or solid attribute point;According to the number of being classified as liquid attribute point and first quantity determine liquid component proportion, compare with preset threshold value, output diabetic foot ulcer grading result.

The present application relates to the technical field of wound dressing, and particularly relates to a chitosan modified clay-based aerogel wound dressing and a preparation method thereof. The preparation method comprises the following steps: S1: adding clay minerals into deionized water to prepare a slurry, and adding acetic acid while stirring to obtain a clay mineral slurry; S2: dissolving chitosan in an acetic acid solution to prepare a chitosan sol; S3: continuously stirring and mixing the clay mineral slurry and the chitosan sol to obtain a sol; S4: immersing the sol in a metal cation solution first, and then immersing the sol in an anion salt solution to obtain a standing product; and S5: freeze-drying the standing product to obtain a composite aerogel. The present application successfully constructs a three-dimensional porous aerogel dressing, which not only retains the strong hemostatic ability of clay, but also significantly enhances the mechanical strength and antibacterial performance, and exhibits great potential in the treatment of high-exudation complex wounds such as diabetic foot ulcers and infected wounds.

To provide the use of immunoregulatory macrophages for treating microvascular or macrovascular disorders of the lower extremities, such as diabetic foot ulcers or venous leg ulcers.SOLUTION: An immunoregulatory macrophage expressing the markers CD258, DHRS9 and IDO for use in a method of treating a microvascular or macrovascular disorder of the lower extremities in a subject is provided. Also provided is a pharmaceutical composition for use in a method of treating a microvascular or macrovascular disorder of the lower limb in a subject comprising said macrophages.SELECTED DRAWING: None

The present application relates to the technical field of medical devices, mainly applied to patients with diabetic foot ulcers or osteomyelitis. Specifically, it is a kind of flushing drainage device, including support frame, the front bottom of support frame is provided with flushing drainage part, flushing drainage part includes two drainage components, the inner wall of two drainage components is provided with positioning assembly. The present application is provided with support frame and flushing drainage part, which realizes the significant improvement of the efficiency and safety of the operation of flushing and drainage of the deep cavity of the patient's bone, which is due to a carefully designed mechanical linkage structure. The structure can automatically complete the extension action of the spray pipe during the preparation stage of flushing, and ensure that the protection plate is quickly and stably closed, which not only ensures the accuracy of the flushing process, but also greatly reduces the risk of secondary injury to the patient during operation. Moreover, through the independent operation and cooperative mechanism of the left and right drainage components, the left component focuses on accurate flushing of the lesion area, and the right component is committed to efficient drainage.

The invention belongs to the technical field of biological medicines, and particularly relates to a flavone-copper complex for promoting healing of diabetic foot ulcer, an external preparation of the flavone-copper complex, and a preparation method and application of the flavone-copper complex. According to the flavone-copper complex disclosed by the invention, a flavone compound and copper ions form the complex, so that the healing of the diabetic foot can be effectively promoted; meanwhile, after the flavonoid compound and the copper ions are compounded, slow release of the copper ions and the flavonoid components can be achieved, the medicine effect is improved, meanwhile, the potential toxicity of the copper ions is reduced, and the flavonoid compound can be directly used as a medicine for relieving ulcer of the diabetic foot.

This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a herbal antibacterial hydrogel for ulcer healing, its preparation method, and its application. The hydrogel is composed of a herbal composition and a matrix. The herbal composition includes dandelion, angelica, scrophularia, forsythia, phellodendron, atractylodes, angelica dahurica, and raw licorice. The matrix contains chitosan, polyvinyl alcohol, water, and glacial acetic acid. The effective components of the herbal medicine are extracted by decoction, concentrated, and freeze-dried to obtain a freeze-dried powder. This powder is then mixed with a chitosan aqueous solution and cross-linked using a liquid nitrogen cyclic freezing method to form the hydrogel. This invention closely addresses the core pathogenesis of diabetic foot ulcers—"blood stasis, dampness, and heat (toxin)"—allowing the various herbs to synergistically exert their effects of clearing heat and detoxifying, promoting blood circulation and removing blood stasis, drying dampness and astringing sores, and nourishing blood and promoting tissue regeneration. The chitosan matrix endows the hydrogel with good biocompatibility, antibacterial properties, and sustained-release drug properties, which can improve the microenvironment of the ulcer wound, inhibit inflammatory responses, and promote granulation tissue growth, effectively promoting the healing of skin sores such as diabetic foot ulcers, and has broad application prospects.

The utility model belongs to the technical field of chronic wound seepage negative pressure drainage, and particularly relates to a chronic wound seepage negative pressure drainage device which comprises a drainage container and a use fixing frame, a negative pressure adjusting device is fixedly installed at the top of the drainage container, and a negative pressure connector is fixedly installed at the top of the negative pressure adjusting device. A flow guide hose is fixedly installed at the top of the negative pressure connector, a drainage tube is fixedly installed on the outer side of the flow guide hose, the negative pressure adjusting device and the negative pressure connector can enable medical staff to set a proper negative pressure value on the controller according to the type of a wound and the healing stage, for example, the negative pressure value can be set to be-80 mmHg to-120 mmHg in the initial stage of diabetic foot ulcer, and the negative pressure value can be set to be-80 mmHg to-120 mmHg in the healing stage of the wound. Along with wound healing, the wound is gradually adjusted to-40 mmHg to-60 mmHg; the wound contact pad is positioned at one end of the drainage tube with the side hole and is in direct contact with a wound; the wound contact pad is made of a hydrophilic polyurethane foam material, and the material can quickly absorb seepage and keep certain air permeability at the same time, so that healing of the wound is facilitated.

The invention provides a diabetic foot ulcer risk prediction method and system based on multi-modal information, and belongs to the technical field of medical artificial intelligence and clinical decision support. According to the method, multi-modal features are extracted by integrating clinical, image, metabolism, wearable equipment and other multi-source data, feature fusion is performed by using a multi-view factorization machine, risk prediction is performed in combination with a deep learning model, and reasoning and personalized intervention recommendation are performed by means of a diabetic foot ulcer knowledge graph. The method overcomes the defects that an existing method depends on a single data source and lacks multi-modal fusion and knowledge guidance, early-stage, accurate and interpretable risk prediction of the diabetic foot ulcer risk is achieved, and the method has important clinical application value.

This invention discloses a traditional Chinese medicine ionopolymer delivery agent, its preparation method, and its application in the prevention of diabetic foot ulcers, belonging to the field of pharmaceutical technology. The traditional Chinese medicine ionopolymer delivery agent comprises a vitamin B1 lipoic acid ionopolymer, safflower, kudzu root, and salvia miltiorrhiza, with a mass ratio of 4:(1-2):(2-4):(2-4). The delivery gel comprises the delivery agent, a gel matrix, and a preservative, with a mass ratio of 4:4:(0.45-0.7). This invention exhibits synergistic effects from multiple components, simultaneously improving local neuropathy, ischemia, hypoxia, and inflammatory responses in diabetic foot ulcers. It demonstrates good transdermal absorption, stable efficacy, and high safety. The preparation method is simple and easily industrialized, filling a gap in related effective topical preparations and possessing extremely high clinical application value.

The application belongs to the technical field of biomedical materials and regenerative medicine, and provides a composite nano hydrogel and application thereof in repair of diabetic wound surface. The three-dimensional porous nano hydrogel framework formed by composite crosslinking of HA-3APBA, PVA and lithium clay nanosheet is used as a carrier, antioxidant small molecules NAC and VC are double-loaded, and inactivated umbilical cord serum is dispersed and compounded. In view of the pathological characteristics of high oxidative stress and sustained inflammation of the diabetic wound surface, the application removes active oxygen through the antioxidant small molecules, remodels the wound microenvironment, protects and cooperates with the umbilical cord serum to play the roles of promoting angiogenesis, collagen deposition and re-epithelialization, and solves the problems of easy inactivation of growth factors, prolonged inflammation and healing stagnation of the diabetic wound surface. Animal experiments show that the composite nano hydrogel significantly improves the healing speed and quality of the diabetic wound surface, and can be used for preparing drugs for treating diabetic foot ulcers, diabetic chronic ulcers and high oxidative stress difficult-to-heal wound surfaces.

The invention discloses a traditional Chinese medicine composition for treating long-term ulceration of trauma and a preparation method thereof, and relates to the technical field of traditional Chinese medicine, and the composition is prepared by compatibility of four medicinal materials including hairyvein agrimony charcoal, dragon bone calcined powder, scorched licorice powder and indigo naturalis powder through a special processing technology. The preparation method adopts a step-by-step temperature control calcining method and an equivalent incremental mixing method, so that stable release of pharmacodynamic substances is ensured. Clinical research shows that the composition can reduce the exudation of a traumatic exudate wound within 7 days by 83%, shorten the healing period of diabetic foot ulcer by 40%, and realize the synergistic effect of antibiosis and tissue regeneration by adjusting the pH value of a wound microenvironment. A quality control system covers 12 core indexes such as tannin and calcium ions, the accuracy is improved by more than 3 times compared with that of a traditional preparation, and a safe and effective traditional Chinese medicine solution is provided for refractory wounds.

This invention discloses a strain of Codonopsis pilosula endophytic fungus that produces extracellular polysaccharides and its applications. This Codonopsis pilosula endophytic fungus is a short-spore bacterium, named... Brevibacillus schisleri CpRG-1. The Codonopsis pilosula endophytic bacteria of the present invention exhibit excellent ability to produce extracellular polysaccharides, producing high concentrations of extracellular polysaccharides, which can greatly reduce the cost of extracting polysaccharides from the medicinal herb Codonopsis pilosula; at the same time, the extracellular polysaccharides produced by the Codonopsis pilosula endophytic bacteria not only have antibacterial and hypoglycemic activities, but also can promote the healing of diabetic foot ulcers and improve the intestinal microbiota, providing new ideas for the development of antibacterial drugs, diabetes prevention foods and drugs, and diabetic foot ulcer treatment foods and drugs.

The invention relates to the field of medical treatment and health, and discloses a diabetic foot ulcer electrical stimulation and medicine synergistic treatment device and a using method, the diabetic foot ulcer electrical stimulation and medicine synergistic treatment device comprises a flexible main body belt, the two sides of the flexible main body belt are both provided with binding belts, the surface of the left binding belt is provided with a hook face hook-and-loop fastener, and the surface of the right binding belt is provided with a suede face hook-and-loop fastener; and the electrical stimulation drug delivery synergistic structure is used for integrating electrical stimulation and transdermal drug delivery, so that the rehabilitation treatment of the foot ulcer is enhanced. Organic fusion of treatment means is achieved through an electrical stimulation drug delivery synergistic structure, and the limitation of single treatment is broken through: a platinum composite flexible electrode and a gold composite flexible electrode are matched with an electrical stimulation control module, wound surface microcirculation can be improved through low-frequency electrical stimulation, and meanwhile, a blood vessel dilating agent stored in a drug storage chamber can further enhance the microcirculation improvement effect; the anti-inflammatory drug can reduce local inflammatory response possibly caused by electrical stimulation, and the synergistic effect of stimulation synergy and drug side effect reduction is achieved.

The invention provides a microcapsule wound dressing containing cannabidiol and a preparation method of the microcapsule wound dressing. The microcapsule wound dressing comprises polyurethane foam and functional microcapsules dispersed in the polyurethane foam. The functional microcapsule comprises a microcapsule matrix and cannabidiol located in the microcapsule matrix. The microcapsule matrix is urea resin. The microcapsule wound dressing has a stable structure in a dry environment, and when the microcapsule wound dressing is in contact with liquid such as blood, tissue fluid and the like, the urea-formaldehyde resin can be broken to release cannabidiol, so that wound healing is promoted. Meanwhile, the polyurethane foam can absorb wound exudate, the wound environment is kept stable, and wound healing is promoted. The microcapsule wound dressing is suitable for chronic wound nursing, such as diabetic foot ulcer, bedsore and the like.

The invention provides a preparation method of loropetalum chinense extract and application of the loropetalum chinense extract in preparation of products for diabetic ulcer, and relates to the technical field of traditional Chinese medicine extraction. The preparation method comprises the following steps: mixing a loropetalum chinense raw material, water and coriolus hirsutus, and carrying out enzymolysis to obtain an enzymolysis product; mixing the enzymolysis product with 80wt%-90wt% of an ethanol solution, and performing ultrasonic-assisted extraction to obtain a component 1; adsorbing the component 1 through macroporous adsorption resin, washing the resin with methanol, then eluting with an elution phase, collecting 10-30 min eluent, combining and drying to obtain the component 1; the elution phase is a mixture of methanol and propylene glycol. The loropetalum chinense extract is prepared through a specific preparation method, and the diabetic foot ulcer treatment effect of the loropetalum chinense extract is remarkably improved.

The invention provides an extraction method of autologous neutrophil physical activation multi-component protein and application of the autologous neutrophil physical activation multi-component protein in chronic infectious wound treatment. According to the method, the neutrophil is physically activated, so that chemical irritant residues are avoided, and the compound protein component rich in MPO, NE, Lysozyme, Lactoferrin and other multiple bactericidal proteins is obtained. And the protein component is directly delivered to the basal part of the chronic wound surface in a multi-point and layered injection mode (1 time / 3 days, 250 [mu] L / time) in a focus area, so that bacterial biofilms and necrotic tissues can be effectively removed, wound debridement, cell migration and matrix remodeling are promoted, and the healing of the chronic infectious wound surface is accelerated. The method disclosed by the invention has the advantages of autologous source, good biocompatibility, accurate treatment and the like, is particularly suitable for treating chronic infectious wounds such as diabetic foot ulcer and the like, and provides a new thought for treating the chronic wounds.

This invention provides the application of exercise-induced sweat extract from healthy individuals in the preparation of a topical medication for diabetic foot ulcers, belonging to the field of biomedicine. Addressing the problems of high cost and limited efficacy of existing treatments, this invention creatively proposes to collect, wash, centrifuge, and filter sweat from healthy individuals induced by specific moderate-intensity aerobic exercise to prepare a sterile extract, which can be used as an active ingredient for topical treatment of diabetic foot ulcers. This sweat extract is rich in various bioactive components. In vitro transcriptomic analysis shows that it can reverse the abnormal gene expression profile associated with diabetic foot ulcers. In vivo animal experiments have confirmed that its topical application can significantly accelerate the healing of diabetic wounds through multi-target synergistic effects of anti-inflammation, angiogenesis, epithelialization, and collagen deposition, with effects comparable to recombinant growth factors. This invention provides a novel treatment strategy that is natural, safe, low-cost, and highly effective, with broad clinical application prospects.

The application discloses a diabetic foot ulcer risk early warning system and method based on a multi-modal sensor, which comprises a multi-modal sensing unit, a preprocessing module, an amplification module, an interference suppression module and a control module; the multi-modal sensing unit, the preprocessing module, the amplification module, the interference suppression module and the control module are electrically connected in sequence; the multi-modal sensing unit is used for collecting foot multi-modal physiological signals; the preprocessing module is used for denoising and feature extraction of the collected signals; the amplification module is used for amplifying the weak difference of the foot physiological signals in the early stage of ulcer; the interference suppression module is used for suppressing signal interference caused by environmental temperature and light; and the control module is used for outputting the processed signals. The overall system structure is reasonable, the power consumption is low, long-term foot sticking can be realized, and the early warning reliability is greatly improved.

The invention relates to the field of diabetic foot ulcer, and discloses a diabetic foot ulcer wound edge skin-derived extracellular vesicle as well as a preparation method and application thereof. The preparation method comprises the following steps: pretreating diabetic foot ulcer wound edge skin tissues to obtain tissue fragments; the method comprises the following steps: mixing type IV collagenase and DNaseI enzyme in a DMEM (Dulbecco Modified Eagle Medium) high-glucose culture medium to prepare a tissue dissociation solution; carrying out primary dissociation by using pancreatin digestive juice, and then carrying out secondary dissociation by using tissue dissociation liquid; sequentially and alternately carrying out gradient size filtration and differential centrifugation on the filtrate, and collecting supernate; performing ultra-high-speed centrifugation, ultra-filtration and gradient needle type filtration on the supernate; and performing ultra-high-speed centrifugation again to obtain the diabetic foot ulcer wound edge skin-derived extracellular vesicles. The concentration of the obtained EVs is 3.29 mg / mL, and the characteristics of delayed healing and insufficient collagen deposition can be stably obtained by injecting the EVs into normal mice.

The invention belongs to the technical field of medicine, and discloses a dressing for treating diabetic foot ulcer, a preparation method and application. The dressing is characterized in that PLGA nanoparticles loaded with deferasirox are fixed on the inner walls of meshes of a silicone mesh stent through a polydopamine adhesion layer; the sodium alginate microspheres encapsulating the WAY 316606 are embedded into the carboxymethyl cellulose matrix, and the surface of the deferasirox-loaded PLGA nanoparticle layer is coated with the sodium alginate microspheres; and after the poloxamer 407 temperature-sensitive hydrogel containing rhPOSTN protein is molded by casting, the poloxamer 407 temperature-sensitive hydrogel containing rhPOSTN protein is coated with the sodium alginate microsphere layer for encapsulating WAY 316606. The thought that skin POSTN + fibroblasts regulate and control diabetic foot skin injury re-healing through iron metabolism regulation and interaction of the skin POSTN + fibroblasts and endothelial cells is proved, a molecular mechanism is disclosed, and a powerful basis is provided for later research.