The application provides a culture method and a
seedling hardening and
transplanting method of Huangjiang
Coptis test-tube seedlings, and belongs to the technical field of
plant tissue culture. The application comprises the following steps: (1) after disinfecting Huangjiang
Coptis explants, the explants are inoculated into MS basic culture medium for culture to obtain sterile seedlings; (2) the sterile seedlings are inoculated into adventitious
bud culture medium for adventitious
bud culture to obtain adventitious buds; (3) the adventitious buds are inoculated into proliferation culture medium for proliferation culture to obtain proliferation seedlings; and (4) the proliferation seedlings are inoculated into rooting culture medium for rooting culture to obtain Huangjiang
Coptis test-tube seedlings. The adventitious
bud culture medium, the proliferation culture medium and the rooting culture medium are used for culturing the Huangjiang Coptis test-tube seedlings, through selection of a
seedling hardening and
transplanting matrix, the proliferation coefficient of the test-tube seedlings reaches 3.0, the rooting rate reaches 96.3%, and the
seedling hardening and
transplanting survival rate reaches 91.5%. The culture method can be used for preservation of Huangjiang Coptis
germplasm resources, and provides a basic guarantee for subsequent improvement of Huangjiang Coptis medicinal material
germplasm.