15 results about "Paliurus" patented technology

The invention provides a culture method and an acclimatization and transplantation method for coptis chinensis test-tube plantlets, and belongs to the technical field of plant tissue culture. The method comprises the following steps: (1) disinfecting a coptis chinensis explant, inoculating the disinfected coptis chinensis explant into an MS basal culture medium, and culturing to obtain a sterile seedling; (2) inoculating the aseptic seedlings into an adventitious bud culture medium for adventitious bud culture to obtain adventitious buds; (3) inoculating the adventitious buds into a proliferation culture medium for proliferation culture to obtain proliferation seedlings; and (4) inoculating the proliferation seedlings into a rooting culture medium for rooting culture to obtain the coptis chinensis test-tube plantlets. When the adventitious bud, proliferation and rooting culture medium is used for culturing the coptis chinensis test-tube plantlet, the proliferation coefficient of the test-tube plantlet reaches 3.0, the rooting rate reaches 96.3%, and the survival rate of acclimatization and transplantation reaches 91.5% through seedling acclimatization and transplantation. The culture method provided by the invention is used for preserving the germplasm resources of the coptis chinensis, and provides a basic guarantee for the germplasm improvement of the subsequent medicinal material coptis chinensis.

This invention discloses a strain of *Carya volvacea* ZZNS-Y2 and its applications, belonging to the field of microbial application technology. The *Carya volvacea* (… Daldinia sp. The preservation number of ZZNS-Y2 is CCTCC NO: M 20241561. The method includes the following steps: *Cyclocarya paliurus* ZZNS-Y2 is cultured on one of the following solid-state fermentation media: corn, potato, corn grits, or rice. After extraction and concentration, the compounds crocusatin C and jasminodiol are separated by silica gel column chromatography, gel column chromatography, and high-performance liquid chromatography. The method of this invention is efficient, pollution-free, has mild reaction conditions, is not limited by plant resources, and is easy to scale up for production.

This invention relates to a rooting agent and seedling cultivation method for promoting rooting of *Cyclocarya paliurus* cuttings, belonging to the field of plant asexual reproduction technology. It solves one of the problems in existing *Cyclocarya paliurus* seedling cultivation: low rooting rate, long rooting time, few and poor-quality adventitious roots, and low transplant survival rate. This invention discloses a rooting agent for promoting rooting of *Cyclocarya paliurus* cuttings, which consists of two components, A and B. Component A consists of potassium indolebutyrate, soluble starch, chlorogenic acid, salicylic acid, and talc; component B consists of indolebutyric acid, sucrose, chlorogenic acid, vitamin C, and talc. Using the above-mentioned rooting agent and the corresponding seedling cultivation method, rapid rooting of *Cyclocarya paliurus* cuttings is achieved, with a rooting rate as high as 93.06%, and a large number and good quality of adventitious roots, resulting in a transplant survival rate of 90.37%.

The invention discloses an extraction method of cyclocarya paliurus, and relates to the technical field of extraction.The extraction method of cyclocarya paliurus comprises the following steps that cyclocarya paliurus leaves are cleaned, dried and smashed; dispersing the crushed cyclocarya paliurus leaves in a citric acid-phosphate buffer solution, and then adding a compound enzyme for enzymolysis; adding a deep-eutectic solvent into supernate after enzymolysis, and extracting under the action of an alternating magnetic field; and carrying out macroporous resin separation, ethanol elution and rotary evaporation concentration on the extracted solution to obtain the cyclocarya paliurus extract. According to the method, the extraction rate of effective components in cyclocarya paliurus is remarkably increased.

The invention provides a construction method of a cyclocarya paliurus root system VIGS instantaneous silencing system. The construction method comprises the following steps: connecting a cyclocarya paliurus CpMYC2 gene with pTRV2 to obtain a recombinant vector pTRV2-CpMYC2; the method comprises the following steps: respectively transforming vectors pTRV2-CpMYC2 and pTRV1 into agrobacterium, so as to obtain agrobacterium containing a recombinant vector pTRV2-CpMYC2 and agrobacterium containing an auxiliary vector pTRV1; the method comprises the following steps: respectively inoculating a pTRV2-CpMYC2-containing agrobacterium tumefaciens and a pTRV1-containing agrobacterium tumefaciens into an LB liquid culture medium; collecting thalli, and resuspending the thalli by using the infection liquid; mixing the two resuspension solutions to obtain a dip dyeing bacterial solution; and infecting the root system of the cyclocarya paliurus with the infection bacterial liquid by adopting a vacuum pumping method or a root injury soaking method. The invention also provides application of the method in verification of cyclocarya paliurus gene functions. The cyclocarya paliurus root system is infected through the method, a target gene silencing system is constructed, and a foundation is laid for cyclocarya paliurus gene function research.

The invention relates to the technical field of cyclocarya paliurus processing, and discloses a cyclocarya paliurus composite solid beverage and a preparation method thereof, and a plant composition comprises the following raw material components: cyclocarya paliurus leaves, rhizoma polygonati, haws and Chinese wolfberry fruits in a mass ratio of 3: 1: (1-3): 1. According to the cyclocarya paliurus beverage, four medicinal and edible plants including cyclocarya paliurus leaves, rhizoma polygonati, haws and Chinese wolfberry fruits are extracted by adopting a water extraction method, the method meets the requirements of related food safety standards of new food raw materials, meanwhile, rhizoma polygonati, haws and Chinese wolfberry fruits are added into the cyclocarya paliurus beverage, the flavor of the cyclocarya paliurus beverage is optimized, and meanwhile, the nutrition of the components is matched; the cyclocarya paliurus composite solid beverage has the advantages that the cyclocarya paliurus composite solid beverage is prepared by mixing four medicinal and edible plant dry extract powder of cyclocarya paliurus leaves, rhizoma polygonati, haws and Chinese wolfberry fruits with sodium carbonate, citric acid and erythritol, and the prepared cyclocarya paliurus composite solid beverage can be quickly dissolved without the conditions that the particles are difficult to dissolve, agglomerate, stick to cup walls and the like when being brewed with warm water.

The present application relates to the technical field of afforestation, and particularly relates to a method for planting elaeagnus pungens in arid and semi-arid regions; the method for planting elaeagnus pungens in arid and semi-arid regions uses elaeagnus pungens seeds and elaeagnus pungens seedlings as materials, uses gaseous water condensing agent and composite organic fertilizer microbial agent, and realizes the survival rate improvement of elaeagnus pungens in arid and semi-arid regions through time sequence planting and double assistant spatial layering complementary cooperation; the present application provides a method for planting elaeagnus pungens in arid and semi-arid regions, uses elaeagnus pungens seeds and elaeagnus pungens seedlings as materials, and through the synergistic cooperation of gaseous water condensing agent and composite organic fertilizer microbial agent and time sequence planting, systematically solves the key problems of single water source, insufficient root development, low survival rate and dependence on irrigation in traditional afforestation, and provides an efficient, low-cost and easy-to-promote new solution for ecological restoration and economic forest construction in arid and semi-arid regions.

The invention provides a construction method of a cyclocarya paliurus leaf VIGS instantaneous silencing system. The construction method comprises the following steps: connecting a CpPDS gene with pTRV2 to obtain a vector pTRV2-CpPDS; the method comprises the following steps: respectively transforming carriers pTRV2-CpPDS and pTRV1 into agrobacterium tumefaciens, and then coating an LB solid culture medium; the method comprises the following steps: respectively inoculating agrobacterium containing a carrier pTRV2-CpPDS and agrobacterium containing a carrier pTRV1 into an LB liquid culture medium; collecting thalli, and resuspending the precipitate by using an infection solution; mixing the two resuspension solutions to obtain a dip dyeing bacterial solution; the leaf backs and leaf stems of the cyclocarya paliurus are scratched, and the cyclocarya paliurus are put into the dip-dyeing bacterial liquid to be soaked, or the scratched parts are wrapped with cotton absorbing the dip-dyeing bacterial liquid. The invention also provides application of the method in verification of cyclocarya paliurus gene functions. Cyclocarya paliurus leaves are infected through the method, a silence system of a target gene is constructed, the expression level of the target gene can be reduced, and a foundation is laid for cyclocarya paliurus gene function research.

This invention discloses a method for cultivating sea urchins that can improve their immunity. The method involves pre-introducing *Cyclocarya paliurus*, *Bacillus subtilis*, and photosynthetic bacteria (*Rhodopseudomonas palustris*) into the cultivation pond to cultivate a symbiotic system of bacteria and algae. During the sea urchin cultivation process, this symbiotic system is maintained by controlling the light intensity and applying specific nutrients. When combined with conventional sea urchin cultivation methods under this system, the immunity of sea urchins can be significantly improved, ultimately achieving the goal of increasing the survival rate and growth rate of the cultivated sea urchins.

The present application belongs to the technical field of natural medicinal chemistry, and particularly relates to a new lignan compound extracted and separated from Cyclobalanopsis glaucoides leaves. Meanwhile, the present application also relates to a method for extracting and separating the new lignan compound from Cyclobalanopsis glaucoides leaves, which comprises the following steps: taking dried Cyclobalanopsis glaucoides leaves, crushing the leaves, and then impregnating and extracting the crushed leaves with ethanol; concentrating the extract, and then extracting the concentrated extract with petroleum ether, ethyl acetate and water-saturated n-butanol in sequence; subjecting the n-butanol extract to D101 macroporous resin, silica gel chromatographic column, Sephadex LH-20 and ODS column chromatography in sequence, and then purifying the new compound by semi-preparative HPLC. In addition, the new compound has obvious inhibitory effect on the generation of NO at a concentration of 10 muM, and the inhibition rate is 52.66%, so that the new compound has good anti-inflammatory activity.

The utility model relates to the technical field of wild jujube seedling digging, in particular to a wild jujube seedling digging device which comprises a main rod, the main rod is of a T-shaped design, and a screw cap assembly is installed on the outer surface of the main rod in a penetrating mode. According to the digging device for the wild jujube seedlings, when the digging device is used, a main rod and a bent rod can be separated only by grasping and pulling an anti-skid handle and a grab handle attaching block, a first inserting piece and a second inserting piece can be separated along with the main rod and the bent rod, then the first inserting piece and the second inserting piece are placed on the two sides of the wild jujube seedlings, and the digging device is convenient to use. The main rod and the bent rod are folded to drive the first inserting piece and the second inserting piece to be combined, then the first inserting piece and the second inserting piece can be inserted into soil through pressing by means of the weight of the body, the soil and seedlings are brought out by shaking the digging device, and the first inserting piece and the second inserting piece are stretched and placed into the soil. The efficiency of placing the seedlings into the digging device is improved, and the digging efficiency is improved.

The application provides a culture method and a seedling hardening and transplanting method of Huangjiang Coptis test-tube seedlings, and belongs to the technical field of plant tissue culture. The application comprises the following steps: (1) after disinfecting Huangjiang Coptis explants, the explants are inoculated into MS basic culture medium for culture to obtain sterile seedlings; (2) the sterile seedlings are inoculated into adventitious bud culture medium for adventitious bud culture to obtain adventitious buds; (3) the adventitious buds are inoculated into proliferation culture medium for proliferation culture to obtain proliferation seedlings; and (4) the proliferation seedlings are inoculated into rooting culture medium for rooting culture to obtain Huangjiang Coptis test-tube seedlings. The adventitious bud culture medium, the proliferation culture medium and the rooting culture medium are used for culturing the Huangjiang Coptis test-tube seedlings, through selection of a seedling hardening and transplanting matrix, the proliferation coefficient of the test-tube seedlings reaches 3.0, the rooting rate reaches 96.3%, and the seedling hardening and transplanting survival rate reaches 91.5%. The culture method can be used for preservation of Huangjiang Coptis germplasm resources, and provides a basic guarantee for subsequent improvement of Huangjiang Coptis medicinal material germplasm.

The invention discloses a sterile cultivation method for mature stems of cyclocarya paliurus, which comprises the following steps: cutting healthy mature branches without diseases and insect pests from robust adult stock plants of cyclocarya paliurus without diseases and insect pests; cleaning the branches, placing the branches in an environment of 4 DEG C, and continuously storing the branches at low temperature for 8 days; cutting the pretreated branches into 3-4cm stem segments with buds, then completely immersing the stem segments in sterile warm water at 37 DEG C for 5 minutes, and repeating the process for 3-4 times; transferring the soaked stem segments with buds into 75% ethanol to soak for 30 seconds, then disinfecting for 5 minutes by using 0.1% ethanol, and washing for 3 times by using sterile water after disinfection is completed, so as to obtain sterile stem segments; the sterilized sterile stem segments are inoculated into a 1 / 2 MS culture medium, 0.3 mg / L of TDZ, 0.01 mg / L of NAA and 0.1 mg / L of NAA are added, adventitious buds are induced to germinate, and a sterilizing reagent comprises a 75% ethanol solution prepared from anhydrous alcohol and water according to the volume ratio of 3: 1 and a 0.1% solution.

The present application relates to the technical field of afforestation, and particularly relates to a method for planting elaeagnus pungens in arid and semi-arid regions; the method for planting elaeagnus pungens in arid and semi-arid regions uses elaeagnus pungens seeds and elaeagnus pungens seedlings as materials, uses gaseous water condensing agent and composite organic fertilizer microbial agent, and realizes the survival rate improvement of elaeagnus pungens in arid and semi-arid regions through time sequence planting and double assistant spatial layering complementary cooperation; the present application provides a method for planting elaeagnus pungens in arid and semi-arid regions, uses elaeagnus pungens seeds and elaeagnus pungens seedlings as materials, and through the synergistic cooperation of gaseous water condensing agent and composite organic fertilizer microbial agent and time sequence planting, systematically solves the key problems of single water source, insufficient root development, low survival rate and dependence on irrigation in traditional afforestation, and provides an efficient, low-cost and easy-to-promote new solution for ecological restoration and economic forest construction in arid and semi-arid regions.

The invention provides a method for transient overexpression of exogenous genes on cyclocarya paliurus leaves, which comprises the following steps: carrying out double enzyme digestion on a target gene and an overexpression vector, and connecting the target gene and the overexpression vector by using ligase; transforming the recombinant overexpression vector into agrobacterium tumefaciens, and coating an LB solid culture medium with the agrobacterium tumefaciens; propagating the monoclone in an LB liquid culture medium; after the agrobacterium tumefaciens is collected, discarding the supernatant, and resuspending with the infection liquid to obtain an agrobacterium tumefaciens infection liquid; the method comprises the following steps: scratching leaf backs and leaf stems of cyclocarya paliurus, infecting leaves with an agrobacterium infection solution, or wrapping scratched parts with cotton absorbing the agrobacterium infection solution for infection. The invention further provides application of the method, and the exogenous gene is the CpSnRK1 alpha gene of the cyclocarya paliurus. The method has the advantages that a simple, convenient and efficient exogenous gene transient overexpression method for cyclocarya paliurus leaves is established for the first time, effectiveness of the method is verified through application of the CpSnRK1alpha gene, and potential possibility is provided for follow-up improvement of cyclocarya paliurus varieties through gene regulation and control.