The invention discloses a sterile cultivation method for mature stems of
cyclocarya paliurus, which comprises the following steps:
cutting healthy mature branches without diseases and
insect pests from robust adult stock plants of
cyclocarya paliurus without diseases and
insect pests; cleaning the branches, placing the branches in an environment of 4 DEG C, and continuously storing the branches at low temperature for 8 days;
cutting the pretreated branches into 3-4cm stem segments with buds, then completely immersing the stem segments in sterile
warm water at 37 DEG C for 5 minutes, and repeating the process for 3-4 times; transferring the soaked stem segments with buds into 75%
ethanol to soak for 30 seconds, then disinfecting for 5 minutes by using 0.1%
ethanol, and washing for 3 times by using
sterile water after disinfection is completed, so as to obtain sterile stem segments; the sterilized sterile stem segments are inoculated into a 1 / 2 MS culture medium, 0.3 mg / L of TDZ, 0.01 mg / L of NAA and 0.1 mg / L of NAA are added, adventitious buds are induced to germinate, and a sterilizing
reagent comprises a 75%
ethanol solution prepared from
anhydrous alcohol and water according to the volume ratio of 3: 1 and a 0.1% solution.