Root promoting agent for promoting rooting of cyclocarya paliurus softwood cuttings, and seedling raising method

Abstract

The present invention relates to a root promoting agent for promoting the rooting of Cyclocarya paliurus softwood cuttings, and a seedling raising method, belonging to the technical field of plant asexual propagation, and solving one of the problems in the prior art of low rooting rate, long rooting time, low number and low quality of adventitious roots, and low transplant survival rate in a process of raising Cyclocarya paliurus seedlings from softwood. Disclosed is a root promoting agent for promoting the rooting of Cyclocarya paliurus softwood cuttings, the root promoting agent being composed of two components, component A and component B; component A is composed of potassium indole butyrate, soluble starch, coronatine, salicylic acid and talcum powder; component B is composed of indolebutyric acid, sucrose, coronatine, vitamin C and talcum powder. Using the described root promoting agent and matching seedling raising method can achieve rapid rooting of Cyclocarya paliurus softwood, with a rooting rate of up to 93.06%, a large number of good quality adventitious roots, and a transplant survival rate of 90.37%.

Description

Root-promoting agent for promoting rooting of Cyclocarya paliurus softwood cuttings and seedling-raising method Technical Field

[0001] The invention relates to the technical field of plant asexual propagation, and in particular to a root-promoting agent for promoting rooting of Cyclocarya paliurus softwood cuttings and a seedling-raising method. Background Art

[0002] Cyclocarya paliurus (Batalin) Iljinsk, a monospecific plant species unique to my country, is a precious tree species with diverse uses, including medicinal, health-promoting, timber, and ornamental properties. Existing Cyclocarya paliurus resources primarily come from natural forests, which are limited in number and scattered in distribution. Natural forest resources have been severely damaged, hampering the development and industrialization of Cyclocarya paliurus. Furthermore, Cyclocarya paliurus seeds, mechanically bound by the seed coat and inhibited by endogenous substances, exhibit deep dormancy, typically germinating after one or two years. They also contain a high proportion of empty seeds and an extremely low natural germination rate, severely limiting the plant's sexual reproduction and hindering resource development.

[0003] With the promotion of improved varieties of Cyclocarya paliurus leaf plantations, research on vegetative propagation techniques has become a key technical issue that urgently needs to be addressed in the development of the Cyclocarya paliurus leaf plantation industry. Currently, vegetative propagation of Cyclocarya paliurus primarily relies on grafting, but this method has a low survival rate and a small propagation coefficient, making it difficult to meet the seedling needs of large-scale plantation cultivation. While progress has been made in tissue culture seedling production, with some improvements in the propagation coefficient, rooting and transplant survival rates remain low.

[0004] Green poplar is a tree species that is difficult to root by cuttings. It has a low rooting rate, a small number of adventitious roots, poor quality of adventitious roots (short root length), and is mainly composed of fleshy roots with few lateral roots. Therefore, the survival rate of transplantation is low. Summary of the Invention

[0005] In view of the above analysis, the embodiments of the present invention aim to provide a root-promoting agent and a seedling raising method for promoting the rooting of young branches of Cyclocarya paliurus cuttings, so as to solve one of the problems in the existing technology of young branches of Cyclocarya paliurus seedling raising, especially in the cutting seedling raising process, such as low rooting rate, long rooting time, small number and poor quality of adventitious roots (mostly fleshy roots), and low transplant survival rate.

[0006] The invention discloses a root-promoting agent for promoting the rooting of Cyclocarya paliurus softwood cuttings. The root-promoting agent consists of two components: component A and component B. Component A consists of potassium indolebutyrate, soluble starch, coronatine, salicylic acid, and talcum powder; and component B consists of indolebutyric acid, sucrose, coronatine, vitamin C, and talcum powder.

[0007] Specifically, the ingredients of component A are 4 to 8 parts by mass of potassium indolebutyrate, 200 to 400 parts of soluble starch, 5 to 15 parts of coronatine, 100 to 150 parts of salicylic acid, and 200 to 500 parts of talc.

[0008] Specifically, the ingredients of component B are 4-8 parts by mass of indolebutyric acid, 200-400 parts by mass of sucrose, 5-15 parts by mass of coronatine, 100-150 parts by mass of vitamin C, and 200-500 parts by mass of talc. Specifically, the mass ratio of component A to component B is (1-4):(4-1).

[0009] Preferably, the mass ratio of component A to component B is 1:1.

[0010] The present invention also discloses a seedling raising method for promoting rooting of cycad paliurus softwood cuttings. The seedling raising method comprises the steps of scion nursery management, cutting bed preparation, cutting preparation and treatment, cutting and post-cutting management. Before cutting, the cuttings are treated with the root promoting agent.

[0011] The seedling raising method comprises the following specific steps:

[0012] S1 Scion nursery management: Select Cyclocarya paliurus seedlings as mother plants for scion collection and cut the stems. After cutting the stems, loosen the soil, remove weeds, and apply urea.

[0013] S2 Cutting bed preparation: Set up the cutting bed and spread the cutting medium evenly on the seedbed, then cover and seal it with film for sterilization;

[0014] S3 Cutting preparation and treatment: Cut semi-lignified branches from the mother plant as cuttings, and cut and disinfect the cuttings;

[0015] S4 cutting: using the root-promoting agent to treat the cuttings, then cutting and building a shed;

[0016] S5 Post-planting management: Control the temperature, relative humidity, and light intensity in the greenhouse, and regularly sterilize, remove dead branches and fallen leaves.

[0017] Specifically, the trunk cutting height in step S1 is 5 to 10 cm above the ground diameter.

[0018] Specifically, the preparation method of the cutting medium described in step S2 is: peat soil, perlite and river sand are mixed in a volume ratio of 2 to 3:1:1 to prepare the cutting medium; when mixing the medium, 50 g each of 50% carbendazim wettable powder and 75% chlorothalonil wettable powder are added per cubic meter of the medium.

[0019] Specifically, the fungicide used in the cutting disinfection operation in step S3 is 2000-3000 times diluted 30% bethamelide aqueous solution and 1000-2000 times diluted 50% carbendazim wettable powder.

[0020] Compared with the prior art, the present invention can achieve at least one of the following beneficial effects:

[0021] 1. Treatment with the disclosed root-promoting agent significantly shortens the rooting time of Cyclocarya paliurus cuttings. In addition to the root-promoting effects of potassium indolebutyrate and indolebutyric acid in the root-promoting agent, the low concentration of coronatine in supplementary materials A and B promotes cell differentiation and accelerates callus formation. Furthermore, the soluble starch and sucrose in the supplementary materials provide a carbon source, accelerating callus formation and root development. Furthermore, the vitamin C in the supplementary materials has a significant antioxidant effect, inhibiting the oxidation of phenolic acids in the Cyclocarya paliurus cuttings to form quinones. The synergistic effect of these ingredients accelerates the rooting process, thereby shortening the time required for rooting.

[0022] 2. After being treated with the root-promoting agent disclosed in the present invention, the cuttings of Cyclocarya paliurus have a high rooting rate, a large number of adventitious roots and lateral roots, and a high-quality root system. The indolebutyric acid in component B of the root-promoting agent can induce the formation of root primordia of the cuttings, promote cell differentiation and division, be beneficial to the generation of new roots and the differentiation of the vascular system, and promote the formation of adventitious roots of the cuttings. The potassium indolebutyrate in component A can promote the cuttings to grow thin and sparse lateral roots and fibrous roots with many branches. Through the mixed use and synergistic effect of component A and component B, the root-promoting agent component A can effectively promote the longitudinal distribution of adventitious roots on the cuttings and increase the number of roots; component B can effectively promote the horizontal expansion of adventitious roots and form more secondary lateral roots, thereby achieving the technical effects of high rooting rate of Cyclocarya paliurus cuttings, a large number of adventitious roots and lateral roots, and a high-quality root system.

[0023] 3. High transplant survival rate for cuttings. Cuttings treated with this root promoter develop a well-developed functional root system with numerous lateral roots. Anatomically, the root system connects to the cuttings to form a complete conductive tissue, ensuring water balance during transplantation. The integrity and abundance of the root system ensure a high transplant survival rate.

[0024] 4. The seedling raising operation is simple and the raw materials are readily available, which is conducive to large-scale promotion. The method for raising seedlings by softwood cuttings of the present invention uses readily available cutting substrate raw materials, is simple and convenient to prepare, and is easy to implement. The fringe length is shorter than that of common softwood cuttings, which can shorten the interval between fringe collection and improve the propagation coefficient. The rooting time of softwood cuttings of Cyclocarya paliurus is shortened, the number of roots is greater, the root system is of high quality, the rooting rate is stable, and the transplant survival rate is high, making it suitable for widespread promotion and application in the Cyclocarya paliurus industry.

[0025] In the present invention, the above-mentioned technical solutions can be combined with each other to achieve more preferred combinations. Other features and advantages of the present invention will be described in the following description, and some advantages will become apparent from the description or be learned through practice of the present invention. The objectives and other advantages of the present invention can be realized and obtained through the contents particularly pointed out in the description and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The accompanying drawings are only for the purpose of illustrating particular embodiments and are not to be considered limiting of the present invention. Like reference symbols denote like parts throughout the drawings.

[0027] FIG1 is a photo of Cyclocarya paliurus seedlings cultivated in Example 1;

[0028] Figure 2 is a photo of Cyclocarya paliurus seedlings (60 days) cultivated in Comparative Example 1;

[0029] FIG3 is a photo of Cyclocarya paliurus seedlings (60 days) cultivated in Comparative Example 2;

[0030] FIG4 is a photo of Cyclocarya paliurus seedlings (60 days) cultivated in Comparative Example 3. DETAILED DESCRIPTION

[0031] The preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings, wherein the accompanying drawings constitute a part of this application and are used together with the embodiments of the present invention to illustrate the principles of the present invention, and are not used to limit the scope of the present invention.

[0032] The invention discloses a root-promoting agent for promoting the rooting of Cyclocarya paliurus softwood cuttings. The root-promoting agent consists of two components: component A and component B. Component A consists of potassium indolebutyrate, soluble starch, coronatine, salicylic acid, and talcum powder; and component B consists of indolebutyric acid, sucrose, coronatine, vitamin C, and talcum powder.

[0033] Component A and Component B contain potassium indolebutyrate and indolebutyric acid, respectively. These two ingredients have different chemical properties. Potassium indolebutyrate is an inorganic salt and is completely water-soluble. However, due to the hygroscopic nature of the talc used in the formulation, it easily absorbs moisture during storage. Indolebutyric acid, on the other hand, has extremely low solubility in water. Therefore, they must be prepared separately and stored away from light, then mixed before use to maintain the activity of potassium indolebutyrate and indolebutyric acid.

[0034] Potassium indolebutyrate: a plant growth regulator that is easily soluble in water, has stable properties, is easily conducted in the body, can promote cell division and cell proliferation, and induce the formation of adventitious roots. Its main function is to promote the growth of thin, sparse, and branched lateral roots and fibrous roots in cuttings, which can effectively avoid the production of fleshy roots.

[0035] Soluble starch: An easily absorbed carbon source that provides nutrients for callus formation, root primordium differentiation, elongation, and lateral root formation during rooting.

[0036] Coronatine: A new plant growth regulator. It can increase anthocyanin production and induce the production of chlorophyllase. It has a wide range of physiological functions, including regulating growth, inhibiting aging, promoting cell differentiation, increasing chlorophyll content, and enhancing plant stress resistance.

[0037] Salicylic acid: A novel plant hormone with physiological regulatory functions. It regulates growth, inhibits aging, and acts as an antioxidant. It not only directly participates in plant growth and development, but also regulates plant growth and development by affecting the levels of other endogenous plant hormones.

[0038] Talc: A high-quality filler containing a large amount of silicon. It is non-toxic, odorless, and highly white, effectively protecting the cut edge of the cuttings.

[0039] Indolebutyric acid: a plant growth regulator. After being absorbed by the cuttings, it is not easily transported in the body and often stays in the incision at the base of the cuttings. It can induce the differentiation and division of cambium cells to produce callus tissue, which is beneficial to the generation of new roots and the differentiation of the vascular system, and promotes the formation of adventitious roots of cuttings.

[0040] Sucrose: An important carbon source. It not only provides nutrients as a carbohydrate for root formation and growth, but also acts like a hormone, inducing root formation and promoting root growth.

[0041] Vitamin C: It can be used as a nutritional supplement and antioxidant. It can enhance the resistance of plants, disinfect and sterilize, inhibit the growth of harmful microorganisms, and promote rooting.

[0042] Synergistic Effect: The plant growth regulators indolebutyric acid and potassium indolebutyrate induce cell differentiation and proliferation in the cambium of Cyclocarya paliurus cuttings, gradually producing callus tissue. Simultaneously, the action of relevant enzymes, combined with the carbon source provided by leaf photosynthetic products and the sucrose and soluble starch in the root promoter, induces root primordia from the callus tissue and forms new roots. The vascular system further differentiates, connecting the new roots with the conductive system of the cuttings. The talc, coronatine, vitamin C, and salicylic acid in the ingredients work synergistically to enhance the cuttings' resistance to stress, disinfect and sterilize them, inhibit the growth of undesirable microorganisms, and prevent oxidative aging, accelerating the rooting process and increasing the rooting rate.

[0043] Specifically, the ingredients of Component A, by weight, include 4-8 parts potassium indolebutyrate, 200-400 parts soluble starch, 5-15 parts coronatine, 100-150 parts salicylic acid, and 200-500 parts talc. For example, the amounts of potassium indolebutyrate are 4, 5, 6, 7, and 8 parts; soluble starch is 200, 230, 270, 310, 340, and 390 parts; coronatine is 5, 7, 11, and 14 parts; salicylic acid is 100, 110, 125, 135, and 147 parts; and talc is 200, 270, 330, 380, 440, and 490 parts.

[0044] If potassium indolebutyrate levels are too low, rooting will be minimal or absent. Excessive levels can cause leaf shrinkage or even leaf drop, inhibiting growth and rooting. Excessive amounts of soluble starch can encapsulate the cutting, hindering water absorption and causing water imbalance within the cutting, which can reduce rooting rates. Coronatine, a growth regulator, can inhibit root growth and the defense mechanisms that rely on stomata and salicylic acid at high concentrations. Excessive amounts of salicylic acid can inhibit plant growth and be toxic. Talc effectively protects the cutting cut, but too low a dosage will not fully encapsulate it. Too high a dosage can prevent the cutting cut from absorbing water, hindering rooting.

[0045] For example, component A may include 4 parts potassium indolebutyrate, 300 parts soluble starch, 10 parts coronatine, 150 parts salicylic acid, and 400 parts talc; or 6 parts potassium indolebutyrate, 200 parts soluble starch, 15 parts coronatine, 120 parts salicylic acid, and 280 parts talc; or 8 parts potassium indolebutyrate, 380 parts soluble starch, 13 parts coronatine, 110 parts salicylic acid, and 220 parts talc.

[0046] Specifically, the ingredients of component B, by mass, are 4-8 parts of indolebutyric acid, 200-400 parts of sucrose, 5-15 parts of coronatine, and 100-150 parts of vitamin C. For example, the indolebutyric acid is present in 4, 5, 6, 7, and 8 parts; the sucrose is present in 200, 230, 270, 310, 340, and 390 parts; the coronatine is present in 5, 7, 11, and 14 parts; and the vitamin C is present in 100, 117, 128, 134, and 146 parts.

[0047] If the indolebutyric acid dosage is too low, rooting will be minimal or non-existent. If the dosage is too high, rooting will be minimal, resulting in deformed or fleshy roots. Excessive supplementation of substances like sucrose, coronatine, and vitamin C is harmful rather than beneficial to the plant. Talc effectively protects the cut surface of the cuttings, but if the dosage is too low, it won't completely cover the cut surface. If the dosage is too high, the cut surface won't absorb water easily, hindering rooting.

[0048] For example, component B may be 4 parts indolebutyric acid, 300 parts sucrose, 13 parts coronatine, 150 parts vitamin C, and 370 parts talc; or 7 parts indolebutyric acid, 380 parts sucrose, 10 parts coronatine, 110 parts vitamin C, and 210 parts talc.

[0049] Specifically, the mass ratio of component A to component B is (1-4): (4-1), and for example, the mass ratio is 1:3, 2:3, 1:1, 3:2, and 4:1. Component A can promote the formation of more adventitious roots on cuttings. According to the results of previous tests, the root promoter component A can effectively promote the longitudinal distribution of adventitious roots on cuttings and increase the number of roots; component B can effectively promote the horizontal expansion of adventitious roots and form more secondary lateral roots. According to the analysis of the experimental results, the mass ratio of component A to component B is (1-4): (4-1), which can effectively improve the rooting rate and rooting quality of cuttings; among them, when the mass ratio of component A to component B is 1:1, the rooting rate is the highest, the root system quality is the best, and the transplanting survival rate is also the highest.

[0050] Preferably, the mass ratio of component A to component B is 1:1.

[0051] Specifically, the root growth promoter is in the form of a powder. Powders are more effective in maintaining effectiveness during preparation, storage, and promotion. During preparation, the two powder components can be mixed in the correct proportions and then used. Indolebutyric acid, potassium indolebutyrate, salicylic acid, and vitamin C solutions are best stored at low temperatures and are susceptible to decomposition and oxidation at high temperatures. Therefore, powders are more convenient during storage, transportation, and promotion.

[0052] The present invention also discloses a seedling raising method for promoting rooting of cycad paliurus softwood cuttings. The seedling raising method comprises the steps of scion nursery management, cutting bed preparation, cutting preparation and treatment, cutting and post-cutting management. Before cutting, the cuttings are treated with the root promoting agent.

[0053] The seedling raising method comprises the following specific steps:

[0054] S1 Scion nursery management: Select Cyclocarya paliurus seedlings as mother plants for scion collection and cut the stems. After cutting the stems, loosen the soil, remove weeds, and apply urea.

[0055] S2 Cutting bed preparation: Set up the cutting bed and spread the cutting medium evenly on the seedbed, then cover and seal it with film for sterilization;

[0056] S3 Cutting preparation and treatment: Cut semi-lignified branches from the mother plant as cuttings, and cut and disinfect the cuttings;

[0057] S4 cutting: using the root-promoting agent to treat the cuttings, then cutting and building a shed;

[0058] S5 Post-planting management: Control the temperature, relative humidity, and light intensity in the greenhouse, and regularly sterilize, remove dead branches and fallen leaves.

[0059] Specifically, step S1 involves selecting healthy, pest-free, one- to two-year-old seedlings from high-quality Cyclocarya paliurus provenances or families as mother plants for cutting. From late February to early March, the mother plants are stemmed, soil loosened and weeded, and 3 to 5 grams of urea per plant are applied. The younger the mother plant, the stronger its rooting ability. Stem cutting allows for the production of more robust cuttings from the root neck, thereby improving the quantity and quality of cuttings.

[0060] Specifically, the stem cutting height in step S1 is 5 to 10 cm above the ground diameter. Cutting low can reduce the height at which adventitious buds germinate, bringing them closer to the root neck. The closer to the root neck, the younger the physiological age, which is more conducive to rooting. Cutting too low will reduce the number of shoots, which cannot meet the needs of large-scale production.

[0061] Specifically, the operation of setting up the slotting bed in step S2 is to build the slotting bed in a colorless transparent glass greenhouse, with a bed width of 1.0 to 1.5 meters and a 10 to 15 cm high baffle set around it to ensure the thickness of the slotting bed and facilitate manual operation; a black ground cloth with a gram weight of 100 to 120 g / m is laid on the bed. 2 , water permeability is 3.5L / (s·m 2 ) to keep the moisture content of the cutting medium stable.

[0062] Specifically, the cutting medium described in step S2 is prepared by mixing peat soil, perlite, and river sand in a volume ratio of 2-3:1:1 to form a cutting medium. The medium comprises an organic matrix and an inorganic matrix, and the ratio ensures water permeability, air permeability, and water retention. During mixing, 50g each of 50% carbendazim wettable powder and 75% chlorothalonil wettable powder is added per cubic meter of the matrix to disinfect the matrix. The matrix thickness is 10-13cm, which matches the length of the cuttings. The matrix moisture content is 60-80% of the field water holding capacity, which is the optimum moisture content for plant growth and ensures both water and air supply.

[0063] Specifically, the operation of step S3 is to cut healthy, pest-free, semi-lignified branches of the current year from the mother plant as cuttings from May to early August (the suitable sampling time for softwood cuttings); make cuttings of the same specification of the green poplar branches, cut the top flat and the bottom obliquely, expand the water absorption area of ​​the lower incision, reduce the water loss area of ​​the upper incision, and maintain the water balance in the body; retain 1 to 2 buds on each cutting, and completely retain 1 to 2 healthy leaflets at the base of the compound leaves; disinfect and sterilize the cuttings with a fungicide, soak for 2 minutes, take out for 2 to 3 minutes to air-dry, and then immediately carry out cuttings. This step can significantly reduce the infection risk of the cuttings.

[0064] Specifically, the cuttings are 8 to 13 cm long, 0.3 to 0.5 cm thick, and the upper incision is 1 to 2 cm away from the upper bud. This cutting specification can minimize material usage and increase the number of cuttings while ensuring survival rate (biological activity).

[0065] Specifically, the fungicides used in the cutting disinfection operation described in step S3 are a 2000-3000-fold dilution of a 30% aqueous solution of hymexazol and a 1000-2000-fold dilution of a 50% wettable powder of carbendazim. Hymexazol is a systemic, highly effective pesticide fungicide, soil disinfectant, and plant growth regulator. It has unique efficacy, is highly effective, low-toxic, and harmless, and is a green, environmentally friendly, high-tech product. It can effectively inhibit the normal growth of pathogenic fungal mycelium or directly kill pathogens, while also promoting plant growth. It also promotes the growth and development of crop roots, rooting and strengthening seedlings, and improving the survival rate of crops. Hymexazol has an extremely high penetration rate and can last up to two weeks. Carbendazim is a broad-spectrum fungicide that is effective in preventing and controlling fungal diseases in a variety of crops. The present invention determines the concentrations of the two fungicides based on their instructions and specific experimental results, and this concentration range achieves an ideal sterilization and disinfection effect.

[0066] Specifically, the operation of step S4 is to use the powder dipping method on the cuttings, and immediately carry out cuttings after fully sticking the root-promoting agent; carry out cuttings on the cutting bed with a plant spacing of 5cm×10cm and a cutting depth of 2~3cm, and then compact the matrix around the cuttings so that the cuttings are closely combined with the matrix; spray the fungicide immediately after cutting and rinse the leaves; after cutting, build a small arch shed with a height of 60~70cm above the bed surface and cover it with 4C plastic film. The plastic film is sealed with the ground on all sides. The above settings are used to maintain the humidity of the cutting matrix and the air humidity. The determination of the plant spacing for cuttings is determined according to the size of the young cuttings of the green willow branches, and the leaves of adjacent cuttings cannot be blocked or in contact with each other; the determination of the cutting depth of 2~3cm is determined according to the rooting position of the adventitious roots, and the general rooting position is about 2cm above the lower incision.

[0067] Specifically, the operation of step S5 is to pay attention to the temperature and humidity of the environment and substrate every day after cutting. The temperature in the arch shed should be maintained at 20-32°C, the relative humidity of the air should be controlled at 85%-90%, and the indoor light intensity should be 30,000-50,000 lux; spray a fungicide once every 10-14 days after cutting, and promptly remove dry, moldy plants and fallen leaves. Maintaining the temperature in the arch shed at 20-32°C is conducive to the formation of cutting callus tissue; controlling the relative humidity of the air at 85%-90% and the indoor light intensity at 30,000-50,000 lux is conducive to leaf photosynthesis while maintaining water balance; the purpose of removing dry, moldy plants and fallen leaves is to provide a good sanitary environment for the retained cuttings and avoid cross infection.

[0068] In actual operation, after cutting, the temperature and humidity monitoring system can be used to monitor the temperature and humidity of the air and cutting medium in the arch shed, and the indoor temperature can be controlled through the greenhouse shading system and water curtains. When the weather is too hot, appropriate spraying can be used to cool down and supplement the air humidity.

[0069] Specifically, the fungicide applied for sterilization in step S5 is 2000-3000 times diluted 30% bethamelide aqueous solution and 1000-2000 times diluted 50% carbendazim wettable powder.

[0070] Example (including comparative example) group:

[0071] In mid-May 2021, cut healthy, pest-free, semi-lignified branches from the current year from the mother plant for cuttings. Cut the Cyclocarya paliurus branches into cuttings of the same size, cutting them flat at the top and beveling them at the bottom. Each cutting retains one to two buds and one to two healthy leaflets at the base of the compound leaves. Cuttings should be 8 to 13 cm long and 0.3 to 0.5 cm thick, with the upper cut 1 to 2 cm from the upper bud. Sterilize the cuttings with a 2000-3000-fold dilution of 30% benomyl aqueous solution and a 1000-2000-fold dilution of 50% carbendazim wettable powder. Soak for 2 minutes, remove and air-dry for 2-3 minutes, and then immediately plant.

[0072] Three rooting agent base materials were selected: commercially available ABT-1 (ABT rooting powder No. 1), component A (each containing: 4 mg potassium indolebutyrate, 400 mg soluble starch, 15 mg coronatine, 150 mg salicylic acid, 431 mg talc), and component B (each containing: 8 mg indolebutyric acid, 400 mg sucrose, 15 mg coronatine, 150 mg vitamin C, 442 mg talc). Six rooting agent formulas (Table 1) were prepared according to mass ratios. Their effects on the rooting of Cyclocarya paliurus cuttings were studied to identify the optimal rooting agent combination for Cyclocarya paliurus cuttings.

[0073] Rooting morphology was observed starting from the 10th day after cutting, and the rooting rate, number of adventitious roots, and root length of the cuttings were counted 60 days later to calculate the rooting quality indicators.

[0074] The rooting quality index is calculated as follows: Average number of adventitious roots = Σn i / N; average root length = Σ[Σ(n Lx L x )] i / Σni；平均总根长=生根率×平均不定根数×平均根长。

[0075] Where N is the rooting cutting, n is the number of adventitious roots, i is the cutting number in each repeated root cutting, L x is the adventitious root length, n Lx The number of adventitious roots classified by root length.

[0076] Table 1 Formula of root-promoting agent for softwood cuttings of Cyclocarya paliurus

[0077]

[0078]

[0079] The cuttings treated in Example 1 (Figure 1) swelled up at the incision and formed callus tissue at 10 days; bud-like adventitious roots appeared at the incision callus tissue at 15 days; a large number of roots were generated at 20-30 days, mainly effective roots, and lateral roots began to appear around 30 days; the root system developed well at 40-60 days, and a large number of lateral roots were produced.

[0080] At the end of 60 days of cutting, the data in Table 2 show that, except for comparative examples 1 to 3, the average rooting rates of the embodiments all reached a relatively high level. Among them, the rooting rate of Example 1 was the most significant, reaching 93.06%. The average number of adventitious roots of the cuttings treated with Example 1 was the highest, reaching 6.83 roots, showing good rooting ability. At the same time, the average root length reached 9.38 cm, and the average total root length was the highest, reaching 59.55 cm. This shows that the rooting quality of the cuttings treated with Example 1 is excellent, with strong rooting ability and growth potential. In addition, the transplant survival rate treated with Example 1 was the highest, reaching 90.37%.

[0081] Table 2 Multiple comparisons of rooting rate and quality indexes of Cyclocarya paliurus cuttings with different formulations at 60 days

[0082]

[0083]

[0084] In summary, the cuttings treated with Example 1 (B series: A series = 1:1) had a short rooting time, a large number of roots and a high rooting rate, and the cuttings had well-developed root systems, many lateral roots, high root quality, and a high transplant survival rate, which was the optimal formula.

[0085] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by any technician familiar with this technical field within the technical scope disclosed by the present invention should be covered by the scope of protection of the present invention.

Claims

1. A root-promoting agent for promoting the rooting of Cyclocarya paliurus softwood cuttings, characterized in that: The root promoting agent consists of two components, component A and component B; component A consists of potassium indolebutyrate, soluble starch, coronatine, salicylic acid, and talcum powder; component B consists of indolebutyric acid, sucrose, coronatine, vitamin C, and talcum powder.

2. The root promoting agent according to claim 1, characterized in that: The ingredients of component A are 4 to 8 parts of potassium indolebutyrate, 200 to 400 parts of soluble starch, 5 to 15 parts of coronatine, 100 to 150 parts of salicylic acid, and 200 to 500 parts of talc, in parts by mass.

3. The root promoting agent according to claim 1, characterized in that: The components of component B are 4 to 8 parts by mass of indolebutyric acid, 200 to 400 parts by mass of sucrose, 5 to 15 parts by mass of coronatine, 100 to 150 parts by mass of vitamin C, and 200 to 500 parts by mass of talc.

4. The root promoting agent according to claim 1, characterized in that: The mass ratio of component A to component B is (1-4):(4-1).

5. The root promoting agent according to claim 1, characterized in that: The mass ratio of component A to component B is 1:

1.

6. A seedling raising method for promoting rooting of softwood cuttings of Cyclocarya paliurus, characterized in that: The seedling raising method comprises the steps of scion nursery management, cutting bed preparation, cutting preparation and treatment, cutting and post-cutting management. Before cutting, the cuttings are treated with the root promoting agent described in any one of claims 1 to 5.

7. The seedling raising method according to claim 6, characterized in that: The specific steps include: S1 Scion nursery management: Select Cyclocarya paliurus seedlings as mother plants for scion collection and cut the stems, loosen the soil, remove weeds and apply urea after cutting the stems; S2 Preparation of cutting bed: Set up the cutting bed and spread the cutting medium evenly on the seedling bed, then cover and seal it with film for sterilization; S3 Cuttings preparation and treatment: Cut semi-lignified branches from the mother plant as cuttings, and cut and disinfect the cuttings; S4 cutting: treating the cuttings with the root promoting agent described in any one of claims 1 to 5, then cutting and building an arch shed; S5 Post-planting management: Control the temperature, relative humidity, and light intensity in the greenhouse, and regularly sterilize, remove dead branches and fallen leaves.

8. The seedling raising method according to claim 7, characterized in that: The trunk cutting height in step S1 is 5 to 10 cm above the ground diameter.

9. The method for raising seedlings according to claim 7, characterized in that: The preparation method of the cutting medium described in step S2 is: peat soil, perlite and river sand are mixed in a volume ratio of 2 to 3:1:1 to prepare the cutting medium; when mixing the medium, 50 g each of 50% carbendazim wettable powder and 75% thiophanate-methyl wettable powder are added per cubic meter of the medium.

10. The seedling raising method according to claim 7, characterized in that: The fungicide used in the cutting disinfection operation in step S3 is 2000-3000 times diluted 30% water solution of benomyl and 1000-2000 times diluted 50% carbendazim wettable powder.

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