1841 results about "Vitamin C" patented technology

The invention discloses a method for extracting extracellular vesicles from dried Sanguisorba officinalis rhizomes and application of the extracellular vesicles, and relates to the technical field of molecular biology and biomedicine.The method comprises the following steps that S1, rehydration is conducted, specifically, the dried Sanguisorba officinalis rhizomes are subjected to liquid nitrogen quick freezing and then ground into powder at the low temperature, the powder and hydration liquid are mixed according to the proportion of 1: 10 (w / v), the mixture is slowly stirred for 2-4 h at the temperature of 4 DEG C, and the extracellular vesicles are obtained; removing large-particle impurities by using sterile gauze or a filter screen, and collecting filtrate; according to the method, through the synergistic effect of multiple enzymolysis and in combination with cellulase, pectinase and the like, the degradation efficiency is remarkably improved, meanwhile, EDTA is chelated with metal ions, and the enzymolysis effect is enhanced. Short-time alternating ultraviolet light is introduced to activate'dormant 'vesicles, so that the yield and the biological activity are improved. Secondly, multiple protection mechanisms, such as vitamin C, EDTA (Ethylene Diamine Tetraacetic Acid), cane sugar, BSA (Bovine Serum Albumin) and an ultraviolet protective agent are adopted, so that the integrity and the biological activity of the vesicles are ensured.

The invention discloses a citron and cichorium intybus composite solid beverage, and relates to the technical field of solid beverages, and the solid beverage comprises the following components: 20-30 parts of a citron extract, 50-70 parts of a cichorium intybus extract, 5-10 parts of erythritol, 1-2 parts of vitamin C, 1-2 parts of probiotic powder, 1-2 parts of citric acid, and 1-2 parts of ginger powder. According to the citron and cichorium intybus composite solid beverage, the cichorium intybus extract in the citron and cichorium intybus composite solid beverage is rich in dietary fibers and can help to improve intestinal health and promote digestion, citron and cichorium intybus contain rich antioxidant components and are beneficial to neutralizing free radicals and slowing down the aging process, and the components in the beverage can help to improve energy and promote digestion. The physical and mental states are improved.

A nutritional composition, typically an infant enterically administered nutritional composition, that comprises a milk fat globule membrane component, at least one human milk oligosaccharide and one additional component or a plurality of additional components where the additional component or components include DHA, ARA, Vitamin E, Vitamin C, and sphingomyelin.

The invention discloses a postharvest mango preservation method based on chitosan, citric acid and electron beam irradiation, and the method specifically comprises the following steps: dissolving chitosan with the deacetylation degree of more than or equal to 85% in a 0.7-0.8% citric acid solution, and adjusting the pH value to 4.4-4.6 to prepare a film coating solution; the method comprises the following steps: dipping mangoes, drying to form a continuous coating film, packaging, carrying out 380-440Gy double-sided irradiation treatment, and finally storing in a storage environment with the temperature of 11 + / -1 DEG C and the humidity of 85 + / -5%. According to the method, through synergistic antibiosis of chitosan and citric acid, low-dose irradiation precise sterilization and low-temperature microenvironment regulation and control, the rotting rate and browning degree of the harvested mangoes are effectively reduced, the storage period is prolonged, the fruit hardness, vitamin C and other nutritional ingredients are maintained, and the method is suitable for large-scale postharvest preservation and long-distance storage and transportation of the mangoes.

The invention provides application of molybdenum disulfide nanoparticles in preparation of a tomato leaf fertilizer, and particularly relates to the technical field of tomato fertilizers. The molybdenum disulfide nano-particles act on tomato plants in a foliage spraying manner, so that the growth of the tomato plants is promoted and / or the fruit quality is improved. The molybdenum disulfide nanoparticles are used as the foliar fertilizer to significantly increase the plant height and stem diameter of tomatoes, increase the chlorophyll content and trace element content of plants, increase the vitamin C content, soluble sugar content and soluble solid content of tomato fruits, and significantly increase the accumulation of Mo and S of plants through the sulfur-molybdenum synergistic effect, so that the yield of the tomatoes is increased. A scientific basis is provided for reasonable application of the nano molybdenum fertilizer, and a way is opened up for high-yield and high-quality cultivation of tomatoes.

The invention provides an antioxidant enzyme-containing composition and a preparation method thereof, and relates to the technical field of enteritis treatment. The preparation method comprises the following steps: S1, collecting various component materials for preparing the antioxidant enzyme-containing composition: collecting various raw materials for preparing glucan, fructo-oligosaccharide, galactooligosaccharide, superoxide dismutase, vitamin C, bunge cherry seed powder, cassia seed powder, turmeric, sorbitol, hydroxypropyl methyl cellulose, silicon dioxide and magnesium stearate; according to the invention, a matched powder bagging machine is adopted to realize full-automatic unattended operation to automatically complete the large bag filling and sealing step in the steps S4-S10, vacuumizing is performed firstly and then sealing is performed, and the storage space is compressed by vacuumizing, so that the condition that the bag is expanded and broken by internal air pressure in the transfer and storage process is not easy to cause; in the bag sealing process, the phenomena of high hot melting efficiency and high sealing speed are achieved by adopting a bag strip guiding and rolling hot melting mode.

The invention discloses a method for efficiently degrading tannin in fruit juice, which comprises the following steps: taking the fruit juice, adding tannase, carrying out enzymolysis for 12-24 hours, carrying out 100-600Mpa ultrahigh pressure treatment for 10-20 minutes, and then continuing to carry out enzymolysis for 12-24 hours. According to the method disclosed by the invention, an ultrahigh pressure combined tannase treatment technology is adopted, and ultrahigh pressure treatment is arranged after the enzymolysis reaction is performed for a period of time, so that the enzymolysis reaction period of tannase is prolonged by utilizing the activation effect of the ultrahigh pressure treatment on enzyme, and the degradation efficiency of tannin can be remarkably improved; meanwhile, the content of heat-sensitive active ingredients such as polyphenol, flavone and vitamin C in the fruit juice can be well retained, the method is suitable for roxburgh rose juice and other fruit juice rich in tannin and heat-sensitive active ingredients, and the taste and quality of the fruit juice can be effectively improved.

The invention discloses a medicinal and edible anti-alcoholism composition and a preparation method thereof, and relates to the crossing field of biotechnology and food and medicine, the composition comprises the following components: dried orange peel powder, vitamin C, glutathione, phellinus igniarius extract, polygonatum sibiricum extract, oriental wormwood extract, isatis root extract, schisandra chinensis extract, ginseng powder, lucid ganoderma extract and licorice extract. Silymarin, puerarin, white tomato powder, watermelon powder, fucoidin, tomato powder, mung bean powder, celery powder, turmeric powder, hawthorn fruit powder, honey powder, corn peptide, soybean peptide powder, xanthan gum and stevioside are added. The composition provided by the invention has the effect of increasing the content of ethanol dehydrogenase and acetaldehyde dehydrogenase in blood after drinking, and has the capability of promoting alcohol metabolism.

The compositions comprise at least two ingredients selected from the group consisting of fish oil, L-arginine, vitamin E, vitamin C, vitamin B3, vitamin B5, vitamin B6, vitamin B1, vitamin B2, folic acid, biotin, vitamin B12, and are formulated for the prevention and / or treatment of a disease or condition associated with muscle decline in a subject in need thereof. Preferably, the subject in need is an individual at risk of or having renal failure, renal dysfunction or / and nephropathy, such as chronic nephropathy, protein energy consumption, end-stage nephropathy, renal failure due to hospitalization in an intensive care unit, acute kidney injury, and the muscle decline is selected from the group consisting of muscle depletion, muscle loss due to renal failure or dysfunction, muscle decline associated with chronic kidney disease, muscle loss due to hospitalization in intensive care units during renal failure, metabolic acidosis during chronic kidney disease, protein energy depletion, or a combination thereof. The method enables the prevention and / or treatment of at least one outcome of a disease or condition associated with muscle decline in a subject at risk of or having renal failure, renal dysfunction or / and kidney disease. The composition and the method can be used to improve the level of at least one amino acid.

The preparation method comprises the following steps: S1, raw material preparation: selecting sodium selenite as a selenium source, and preparing konjac glucomannan and vitamin C as reducing agents at the same time; the molar ratio of the selected sodium selenite to the selected konjac glucomannan to the selected vitamin C is 1: (0.5-2): (2-5); s2, solution preparation: dissolving sodium selenite in deionized water to prepare a sodium selenite solution with the concentration of 0.1-0.5 mol / L; according to the preparation method of the nano-selenium fertilizer, through the nano-selenium fertilizer prepared through the steps S1 to S6, the particle size of nano-selenium particles can be distributed between 30 nm and 80 nm, so that the nano-selenium fertilizer has good dispersity and stability and is conveniently and efficiently applied to agricultural production, and the effect of improving the selenium absorption and utilization efficiency of crops is achieved; and the preparation method is simple and easy to implement, low in cost, green and environmentally friendly.

The invention provides an acne-removing composition containing ceramide, a preparation method of the acne-removing composition and an acne-removing product, and the acne-removing composition is prepared from the following components in parts by weight: 5 to 10 parts of plant extract, 1 to 3 parts of ceramide, 1 to 3 parts of gentiopicroside-vitamin C eutectic, 1 to 3 parts of compound polysaccharide and 0.3 to 0.7 part of transdermal enhancer. The plant extract is prepared from a herba dendrobii fruticosae flower extract, a lasiocarpus trifoliatus extract and a hedera rhododendron extract in a mass ratio of 1: (1-3): (2-4); the compound polysaccharide is prepared from tremella polysaccharide, tamarind seed polysaccharide and ganoderma lucidum polysaccharide in a ratio of 1: (3-5): (0.5-0.8); the transdermal enhancer is prepared from a cucumber extract and a salvia miltiorrhiza root extract in a mass ratio of 1: (1-3). The plant extracts in the acne-removing composition take effect synergistically, the acne-removing effect is achieved from multiple aspects of bacteriostasis, anti-inflammation and oil control, the purpose of efficiently removing acnes without relapse is achieved, and the natural plant extracts are mild and non-irritant, do not cause burden to the skin and have good application prospects.

The present invention provides a pharmaceutical composition, the composition comprises a therapeutically effective amount of bumetanib, one or more specific antioxidants and other necessary one or more pharmaceutically acceptable pharmaceutic adjuvants, and the specific antioxidants are selected from one or more of fat-soluble vitamin C derivatives or phenolic antioxidants. In addition, the invention also discloses a method for improving the stability of the bumetanib pharmaceutical composition. According to the technical scheme, the problems of generation and growth of N-nitroso bumetanib and other related substances in the production and storage process of the bumetanib solid preparation are effectively solved, and the drug stability and the medication safety are remarkably improved.

The invention relates to the technical field of freeze-dried powder preparation, and discloses a white tomato exosome freeze-dried powder preparation method, which comprises: 1, manually picking white tomatoes 3-5 days after a color changing period, carrying out double-frequency ultrasonic cleaning, and pre-cooling in a 4 DEG C phosphate buffer solution containing 0.1% of vitamin C; according to the method, by virtue of an ultrasonic-cellulase synergistic crushing process, cells are mildly and efficiently crushed, so that the damage to an exosome structure is reduced, and the release efficiency and activity of the exosome are improved; the zebra fish embryo melanogenesis can be obviously inhibited, and the whitening and freckle-removing effects are relatively good.

The present invention belongs to the technical field of biomass resource utilization and microbial fertilizers, and specifically relates to a fertilizer based on oil-tea camellia cattail, a preparation method and its application. The fertilizer described in the present invention is prepared by a collaborative fermentation process of Aspergillus niger, Phanerochaete chrysosporium and Bacillus subtilis. The obtained fermentation product is separated into solid and liquid to obtain a solid, the solid being a solid fertilizer, and the liquid being a foliar fertilizer after post-treatment. Both can be used for the cultivation of crops such as tangerines and oil-tea camellias. Based on the microbial collaborative fermentation process, the content of anti-nutritional factors tannin and tea saponin in the obtained solid fertilizer is significantly reduced, which is less than 1.8 g / kg and 0.1% respectively on a dry basis, while maintaining a seed germination index higher than 95%. According to a planting comparative test, the average yield per mu of oil-tea camellias using the solid fertilizer and liquid fertilizer obtained in this application increased by 12%. The average yield per mu of tangerines increased by about 11%, and the sugar, vitamin C, and trace element contents in tangerines were higher than those using traditional fertilizers.

The invention relates to the technical field of biological products, and discloses a mesenchymal stem cell hair growth liquid and a preparation method thereof, and the mesenchymal stem cell hair growth liquid comprises the following components: hair follicle mesenchymal stem cells; a hair follicle mesenchymal stem cell exosome; growth factors: a vascular endothelial growth factor (VEGF), a fibroblast growth factor (FGFs), a keratinocyte growth factor (KGF-7) and interleukin-10 (IL-10); vitamins: vitamin E, vitamin B5, vitamin B2, vitamin B6, vitamin C and biotin; the plant extracts comprise a cacumen biotae extract, a polygonum multiflorum extract, a ginseng extract, a peppermint extract, a purslane extract and a rhodiola rosea extract; other active ingredients: tripeptide and digoxin; the auxiliary material is a hyaluronic acid-collagen peptide graft copolymer; and the balance of a phosphate buffer solution.

The invention relates to the field of emulsions for grainy paper coatings, and discloses a preparation method of a water-based environment-friendly emulsion for a grainy paper coating. The preparation method comprises the following steps: S1, preparing an acrylic carbamate modified monomer from hydroxyethyl acrylate, 2, 4-toluene diisocyanate and diethanol amine; and S2, preparing to obtain the emulsion. S3, obtaining the initiator. S4, deionized water, lauryl sodium sulfate, the emulsion and an initiator are subjected to a polymerization reaction, and an emulsion A is obtained. S5, transferring the emulsion A into a degassing kettle, adding tert-butyl hydroperoxide and vitamin C, and performing oxidation-reduction reaction to obtain emulsion B; and S6, carrying out steam degassing on the emulsion B to obtain the water-based environment-friendly emulsion. The water-based environment-friendly emulsion which is good in stability, glossiness, adhesion fastness, water resistance and weather resistance and low in volatile organic compound content can be prepared, and the preparation method is simple, efficient and environmentally friendly and has wide application prospects and market value.

The invention relates to the technical field of pepper planting, and discloses a pepper planting method for improving pepper quality, which comprises the following steps: soil and matrix treatment, seed treatment and sowing, field management and regulation, fertilization strategy, pest control and ecological balance, and plant pruning and fruit optimization. According to the pepper planting method, through the synergistic effect of all links, the pepper planting effect and quality are comprehensively improved from soil improvement to seed treatment, from field management to pest and disease control and then to plant pruning and fruit optimization. Compared with a traditional planting method, the quality indexes such as vitamin C content and capsaicin content of the chilies planted through the method are remarkably improved, the growth cycle is shortened, the yield is increased, meanwhile, use of chemical pesticides is reduced, and the method is environmentally friendly, has remarkable economic, social and ecological benefits and is worthy of popularization and application. Powerful support and guarantee are provided for sustainable development of the pepper planting industry.

The invention discloses a lactobacillus rhamnosus capable of improving joint inflammation and promoting bone health, a metagen and application of the lactobacillus rhamnosus, the lactobacillus rhamnosus is lactobacillus rhamnosus BN02, the lactobacillus rhamnosus is preserved in China General Microbiological Culture Collection Center on November 27, 2023, and the preservation number is CGMCC No.29133. The lactobacillus rhamnosus can be used for improving joint inflammation and promoting bone health. The lactobacillus rhamnosus BN02 can reduce the number of gram-negative bacteria in intestinal tracts of arthritis patients, increase the synthesis amount of bone collagen, and is beneficial to promoting absorption of vitamin C by the intestinal tracts, improving joint inflammation and promoting bone health.

The invention discloses an oligopeptide from undaria pinnatifida and application thereof. The amino acid sequence of the oligopeptide is YRKD, and the molecular weight is 580.63 g / mol. The polypeptide is identified from undaria pinnatifida through a high performance liquid chromatography technology for the first time, and an unreported oligopeptide capable of inhibiting tyrosinase and cooperating with vitamin C to synergistically inhibit tyrosinase is screened out. The oligopeptide derived from undaria pinnatifida is synthesized by a solid phase method, has the characteristics of safety, no toxicity, good water solubility and good stability, and is combined with vitamin C to generate a synergistic effect and synergistically increase the inhibition effect on tyrosinase, so that the oligopeptide can achieve a better tyrosinase inhibition effect at a lower and more stable concentration; the use concentration of the oligopeptide can be greatly reduced, the stability is enhanced, and the oligopeptide can be widely applied to preparation of cosmetics, medicines, health-care products or food additives, and has important economic value and social significance.

The invention discloses a preparation method of a vitamin C liposome preparation capable of improving encapsulation efficiency, the vitamin C liposome preparation is prepared by an ethanol injection-high pressure microjet circulation homogenization method, the homogenized liposome is small in particle size and uniform in distribution, and meanwhile, by combining a dialysis purification and buffer agent pH regulation process, the encapsulation efficiency of the vitamin C liposome preparation is improved. The prepared vitamin C liposome preparation can still keep high stability under the condition that cholesterol is not contained, and the problem that in the prior art, a vitamin C liposome preparation is low in encapsulation efficiency is effectively solved.

The invention discloses a synthesis process of vitamin C ethyl ether, and belongs to the technical field of vitamin C derivative synthesis. The process comprises the following steps: taking L-ascorbic acid as a raw material, adding acetone, anhydrous cupric sulfate and acetyl chloride, and reacting for 1-2 hours at 38-40 DEG C under the protection of nitrogen to obtain a vitamin C protector; adding alkali, water, an alkylating reagent and absolute ethyl alcohol into the reaction product, reacting for 3 hours at 75-78 DEG C under the protection of nitrogen, and performing reduced pressure distillation to obtain a vitamin C protector etherate; and finally, hydrolyzing by using cationic resin, removing a protecting group, and recrystallizing by using ethyl acetate to obtain the vitamin C ethyl ether. Through the synergistic catalysis of anhydrous cupric sulfate and acetyl chloride, the reaction time is remarkably shortened, and the product purity and yield are improved. Through detection, the product purity is more than 99.5%, the total yield can reach more than 80%, and an efficient and economical method is provided for industrial production of vitamin C ethyl ether.

The invention belongs to the technical field of bioengineering, and particularly relates to a sucrose phosphorylase mutant and application thereof in synthesis of 2-O-alpha-D-glucopyranosyl-L-ascorbic acid. The sucrose phosphorylase mutant is obtained by simultaneously mutating multiple sites of amino acids at the 91st site, the 154th site, the 185th site, the 323rd site and the 424th site of wild sucrose phosphorylase derived from Bifidobacterium pseudolongum, so that the efficiency of producing 2-O-alpha-D-glucopyranosyl-L-ascorbic acid by taking sucrose and vitamin C as raw materials is remarkably improved; only 15 hours are needed for completely catalyzing 1.2 M of vitamin C to produce 2-O-alpha-D-glucopyranosyl-L-ascorbic acid; and the yield of the 2-O-alpha-D-glucopyranosyl-L-ascorbic acid reaches 358 g / L. Therefore, the 2-O-alpha-D-glucopyranosyl-L-ascorbic acid has an important application value in the field of production of the 2-O-alpha-D-

The invention belongs to the technical field of biological medicines, and particularly relates to coumarin hydrazone derivatives with antioxidant activity and application thereof. The chemical structural formula of the coumarin derivative is as shown in formula (I), wherein R is aromatic rings containing different substituent groups. An in-vitro anti-oxidation experiment result shows that most of the derivatives have certain scavenging capacity on free radicals such as DPPH, ABTS, superoxide anions and hydroxyl and have certain reducing capacity. It is worthy of noting that the scavenging effect of the derivatives 5a-5d, 5i-5k, 5m and 5n on superoxide anion free radicals is superior to or equivalent to that of vitamin C. The method for preparing the coumarin hydrazone derivative has the advantages of simplicity in operation, convenience in post-treatment, high product separation yield and the like. The coumarin compound can be used for preparing an antioxidant. # imgabs0 #

The invention provides a method for detecting vitamin C based on oxidase active nano enzyme. The method comprises the following steps: preparing a first mixture formed by mixing MnO2 (at) MIP nano-enzyme, a TMB solution, a HAc-NaAc buffer solution and a sample to be detected, and a second mixture formed by mixing MnO2 (at) MIP nano-enzyme, a TMB solution, a HAc-NaAc buffer solution and vitamin C with different concentration gradients; reacting the first mixture and the second mixture under the same predetermined reaction condition, and measuring the absorbance of a product after reaction under a predetermined wavelength to respectively correspond to absorbance values A1 and A0; obtaining a linear regression equation based on the A0 and the vitamin C with different concentration gradients, and determining the content of the vitamin C in the sample to be detected based on the linear regression equation and the A1. The method provided by the invention is rapid in detection and extremely high in sensitivity.

The invention provides a composition for assisting in reducing blood sugar and improving insulin resistance and a preparation method of the composition, and relates to the technical field of functional fiber processing. The composition for assisting in reducing blood sugar and improving insulin resistance comprises the following raw materials in parts by weight: 10-15 parts of depolymerization type composite cereal fibers, 3-5 parts of konjac fibers, 3-5 parts of psyllium husk powder, 2-3 parts of sesame powder, 1-2 parts of xylitol and 0.1-0.5 part of vitamin C, the mass ratio of wheat fiber to corn fiber to sweet potato fiber to pea fiber in the depolymerization type composite grain fiber is (25-30): (35-40): (20-25): (10-15). The composition disclosed by the invention can assist in reducing blood sugar and has a good effect of improving insulin resistance.

A preparation method of a polyester nanofiltration membrane with high chlorine resistance and pollution resistance relates to the technical field of chlorine-resistant polyester nanofiltration membranes, and comprises the following steps: sequentially dipping a polyacrylonitrile support layer in an aqueous phase solution and an oil phase solution, carrying out condensation polymerization at 25-45 DEG C to generate a polyester selection layer on the surface, and finally heating and crosslinking at 45-65 DEG C to obtain the polyester nanofiltration membrane. The aqueous phase solution is a tannic acid aqueous solution, a rhamnose aqueous solution, a vitamin C aqueous solution or a chlorogenic acid aqueous solution, the preparation method is simple and low in cost, and the formed polyester nanofiltration membrane has high chlorine resistance and pollution resistance.

The invention belongs to the technical field of plant genetic engineering, discloses a method for increasing the yield of rice, and aims to solve the technical problem that the yield is reduced due to reduction of photosynthetic efficiency caused by light inhibition of rice under high-light-intensity illumination. According to the method, the expression quantity of the OsJAB1 gene in a target plant is increased, the content of vitamin C in the plant is increased, the photosynthetic electron transfer efficiency is improved, dry matter accumulation and grain development are promoted, and finally the rice yield is increased.

The invention relates to the technical field of biological preparations, and discloses an efficient body function repairing active small molecule peptide preparation and a preparation method thereof.The efficient body function repairing active small molecule peptide preparation is prepared from 15-22 parts of collagen peptide, 5-9 parts of glutathione, 10-18 parts of whey protein peptide, 5-8 parts of vitamin C, 2-4 parts of vitamin E, 1-3 parts of zinc element, 5-15 parts of astragalus membranaceus extract, 3-8 parts of probiotics and 10-20 parts of a delivery carrier. The molecular weight range of the original protein peptide, the glutathione and the whey protein peptide is 500 to 2000Da. According to the invention, collagen peptide, glutathione, whey protein peptide, vitamins and other components are proportioned to realize an anti-oxidation-repair-anti-inflammatory synergistic effect, and vitamin C, vitamin E, zinc element and an astragalus extract are added to cover multi-dimensional repair of an immune system, so that comprehensive body function repair is realized.

A test strip for detecting uric acid in saliva that meets the requirement for room-temperature preservation, a preparation and detection method therefor and the use thereof. The test strip for detecting uric acid in saliva comprises a uricase, a peroxidase, a vitamin C oxidase, a bilirubin oxidase, a color developing agent, and a protective film coated on the test strip for detecting uric acid in saliva. To meet the needs of a target population for long-term monitoring and real-time tracking of changes in their uric acid levels, by means of agent addition, the test strip for detecting uric acid in saliva has prolonged preservation time at room temperature, enables non-invasive, efficient and convenient monitoring of a health status at any time, and timely detection and treatment, allows intuitive visual identification of uric acid levels in saliva by a subject, and meets the needs of a subject for long-term preservation at room temperature.

The invention relates to a high-water-solubility denitroflavin covalent complex as well as a preparation method and application thereof, and belongs to the technical field of organic synthesis and medicinal chemistry. Comprising the following steps: (1) firstly, carrying out bromination reaction on 3-methyl-10-ethyl denitroflavin, so as to obtain 3-methyl-10-(1-bromoethyl) denitroflavin; and (2) coupling the 3-methyl-10-(1-bromoethyl) denitroflavin with vitamin C, so as to obtain the high-water-solubility denitroflavin covalent complex. According to the present invention, the prepared highly water-soluble denitrificin covalent complex has the two-phase solubility of the organic solvent and the water, such that the water solubility of the 3-methyl-10-ethyl-denitrificin is substantially enhanced, and the application range of the denitrificin is expanded. And meanwhile, the denitrified flavin covalent compound also has good antioxidant activity, and the antioxidant duration can be prolonged.