635 results about "Chondroitin" patented technology

The invention discloses a preparation method of injectable nano composite hydrogel for relieving osteoarthritis, which comprises the following steps: firstly, synthesizing nano rod-like hydroxyapatite with the length of 150-300 nm and the diameter of 10-15 nm, and the hydroxyapatite is hydrophilic hydroxyapatite doped with strontium and europium ions; the preparation method comprises the following steps: by taking Ca (NO3) 2, Sr (NO3) 2, Eu (NO3) 3 and soluble phosphate as main raw materials, carrying out hydrothermal reaction to prepare strontium and europium ion co-doped hydrophobic hydroxyapatite, dispersing the strontium and europium ion co-doped hydrophobic hydroxyapatite in a Tris-HCl buffer solution, adding tannic acid, stirring, centrifugally collecting precipitate, and drying to obtain strontium and europium ion doped hydrophilic hydroxyapatite; the preparation method comprises the following steps: taking chondroitin sulfate and adipic dihydrazide as main raw materials, stirring, dialyzing, freeze-drying, and dissolving in a buffer solution to obtain the chondroitin sulfate solution grafted with the hydrazide group; the preparation method comprises the following steps: taking hyaluronic acid, 3-aminophenylboronic acid and sodium periodate as main raw materials, stirring, dialyzing, freeze-drying, and dissolving in a buffer solution to obtain the phenylboronic acid group grafted oxidized hyaluronic acid solution; the preparation method comprises the following steps: blending hydrophilic hydroxyapatite doped with strontium and europium ions, a chondroitin sulfate solution grafted with a hydrazide group, an oxidized hyaluronic acid solution grafted with a phenylboronic acid group and a recombinant collagen solution, and stirring to obtain the injectable nano composite hydrogel. The injectable nano composite hydrogel obtained by the invention has good biocompatibility, and a favorable local immune microenvironment can be created to immunomodulate macrophages so as to relieve osteoarthritis and promote subchondral bone repair.

The invention belongs to the technical field of chondroitin sulfate biosynthesis, and discloses a recombinant strain for high-yield chondroitin sulfate, application of the recombinant strain and a preparation method of the chondroitin sulfate. The recombinant strain provided by the invention can be used for simultaneously expressing UDP-glucosamine isomerase, UDP-glucose dehydrogenase, chondroitin polymerase, glucosamine synthetase, phosphoglucosamine mutase, UDP-N-acetylglucosamine pyrophosphorylase, glucose-6-phosphate isomerase and glucose phosphate mutase; according to the present invention, glucose-1-phosphate uridyl transferase, ATP sulfonase, sulfotransferase, pyruvate kinase and APS kinase are adopted, and the enzyme molecules are matched with the original carbon metabolism pathway in the recombinant strain cell to jointly form the enzyme-linked pathway having the efficient CSA synthesis ability, such that the high-yield and high-purity preparation of the CSA can be achieved; the method has the advantages of simple operation, high biological safety of the obtained product, easy realization of large-scale production and the like, and has a good application prospect.

The invention relates to an oral mucosa repairing medicine and a preparation method thereof, and belongs to the technical field of medicines, the medicine comprises barnacle peptide, salivary lactobacillus, resveratrol, a biphasic adhesion matrix, hydroxyapatite, vitamin B family and other components. The preparation method comprises the following steps: sequentially carrying out enzymolysis on barnacle gooseneck by pepsin and lumbrukinase to obtain barnacle peptide. The double-phase adhesion matrix comprises a water-phase matrix, an oil-phase matrix and lecithin; the water-phase matrix contains a chondroitin sulfate-chitosan quaternary ammonium salt compound, sodium alginate and other components; the oil-phase matrix comprises sesame oil, beeswax, a polylactic acid-glycolic acid copolymer and the like. According to the invention, barnacle peptide and salivary lactobacillus are prepared by adopting a special method and are matched with resveratrol for use, so that multi-target and multi-path promotion of oral mucosa repair can be realized; the chondroitin sulfate-chitosan quaternary ammonium salt compound is prepared through electrostatic and physical embedding, the chondroitin sulfate-chitosan quaternary ammonium salt compound is matched with other components to prepare the biphasic adhesion matrix, the biocompatibility is good, the adhesion is high, and the action time of a sustained-release drug is well prolonged.

The invention relates to injectable hydrogel for rapid hemostasis and antibiosis of an oral cavity as well as a preparation method and application of the injectable hydrogel, and the injectable hydrogel comprises the following components in percentage by weight: 5-15% of aminated chondroitin sulfate, 5-15% of oxidized dextran, 1-3% of platelet-rich fibrin and 0.5-1% of quercetin modified zeolite imidazole acid framework nanoparticles. According to the invention, Chs-ADH and ODex are adopted as matrix materials, PRF and ZIF-8 (at) QCT are introduced, the hydrogel is endowed with antibacterial, anti-oxidation and immunoregulation functions, and by optimizing the proportion of the components, the obtained hydrogel has good injectability, stability and degradation resistance, so that the hydrogel can still maintain an effective blood coagulation function in a high-humidity and high-enzymatic-activity oral environment.

The invention discloses a preparation method and application of chondroitin sulfate sodium, and belongs to the technical field of chondroitin sulfate. The preparation method of the sodium chondroitin sulfate comprises the following steps: dispersing sodium chondroitin which is not sulfated by using an organic solvent, adding a sulfonating reagent for sulfonation reaction, terminating the reaction, centrifuging, and collecting precipitate; and decoloring and drying the collected precipitate to obtain the chondroitin sulfate sodium. The average molecular weight of the final product obtained by the method is controllable, the 6-site overall sulfonation rate is up to 90% or above, the 6-site independent sulfonation rate is up to 60%-85%, and the quality of products among batches is stable. The sodium chondroitin sulfate based on the characteristics is more suitable for being absorbed by a human body, high in bioavailability and higher in safety and effectiveness; the method can be realized without a complex extraction and purification process, and is rich in source, uniform in structure, stable in quality, mild in reaction, reasonable and controllable in production cycle and suitable for large-scale commercial production and application.

The invention relates to a low-molecular-weight chondroitin sulfate composition as well as a preparation method and application thereof, and aims to solve the problems that a chondroitin sulfate product obtained by a single degradation mode is limited in function and difficult to synergistically regulate intestinal barriers. The preparation method comprises the following steps: firstly, preparing LMCSO through oxidative degradation; secondly, preparing LMCSH through hydrothermal degradation; and finally, the LMCSD is prepared through deamination degradation. Finally, the obtained LMCSO, LMCSH and LMCSD are compounded according to the weight ratio of 5: 3: 2, and the low-molecular-weight chondroitin sulfate composition LMCSs-C is obtained. The composition LMCSs-C prepared by the invention can synergistically improve the intestinal barrier function through multiple action mechanisms, not only shows an obvious intestinal barrier repair effect in vitro and animal models, but also has good biocompatibility and safety, and shows a wide application prospect in intestinal health maintenance and related disease intervention.

The invention belongs to the technical field of biological medicine, and particularly relates to a chondroitin sulfate oligosaccharide derivative and a preparation method and application thereof.The structure of the chondroitin sulfate oligosaccharide derivative is shown in the formula I # imgabs0 #, in the formula I, R1 is SO3H and R2 is H or R1 is H and R2 is SO3H, and the polymerization degree of the chondroitin sulfate oligosaccharide derivative is equal to 8; the chondroitin sulfate oligosaccharide derivative has good chronic hepatitis repair promotion and anti-inflammatory activity, is clear in structure, and can be orally taken to treat the non-alcoholic fatty liver disease.

The present application belongs to the field of biomedicine technology, and specifically relates to a chondroitin sulfate oligosaccharide derivative and a preparation method and application thereof. The structure of the chondroitin sulfate oligosaccharide derivative is shown in Formula I, #imgabs0#Formula I, where R1 is SO3H, R2 is H or R1 is H, R2 is SO3H, and the polymerization degree of the chondroitin sulfate oligosaccharide derivative is equal to 8; the chondroitin sulfate oligosaccharide derivative has good promotion of chronic hepatitis repair and anti-inflammatory activity, and has a clear structure, and can be used for oral treatment of non-alcoholic fatty liver disease.

The invention relates to the technical field of biomedicine, and discloses a human metapneumovirus culture method which comprises the following steps: a culture medium is prepared and put into a culture bottle, and the culture medium comprises a DMEM culture medium, glucose, sodium bicarbonate, an HEPES buffer solution, chondroitin sulfate and hyaluronic acid; host cells are selected; preparing a culture solution, wherein the culture solution comprises L-glutamine, bovine serum albumin, antibiotics and N-acetylcysteine; and inoculating the human metapneumovirus into the culture medium for adsorption and infection. The chondroitin sulfate and the hyaluronic acid are added into the culture medium, the chondroitin sulfate and the hyaluronic acid can be combined with glycosaminoglycans on the surfaces of the host cells to form a compound, interaction sites of viruses and the host cells are increased, then virus receptor expression on the surfaces of the host cells is enhanced, the cell membrane environment is optimized, and therefore the anti-tumor effect is achieved. Therefore, the attachment and entry efficiency of the hMPV is improved, and the rapid proliferation of the virus is obviously promoted.

The invention discloses nano-composite hydrogel based on active oxygen removal as well as a preparation method and application of the nano-composite hydrogel, and relates to the technical field of biomedicine. The nano composite hydrogel based on active oxygen removal comprises a three-dimensional network skeleton structure formed by cross-linking oxidized chondroitin sulfate and carboxymethyl chitosan, and melatonin-loaded mesoporous polydopamine nanoparticles are encapsulated in the three-dimensional network skeleton structure. According to the invention, a nano material is combined with a three-dimensional network skeleton structure of the hydrogel, a novel nano composite hydrogel is developed, and the nano composite hydrogel has self-healing ability, good biocompatibility, good mechanical properties and long-acting drug release ability; meanwhile, the melatonin is loaded into the nano-composite hydrogel to develop a medicine compound, the medicine activity and the treatment effect are better than those of the melatonin, inflammation and oxidative stress can be relieved more effectively, and symptoms can be improved more effectively.

The present disclosure relates to a membrane prepared from a blend comprising i) a polysulfone, polyether sulf one or polyarylethersulfone and ii) polyvinylpyrrolidone, characterized in that the membrane is coated with polydopamine and chon-droitin. Optionally the coating further comprises heparin. The disclosure further relates to a process for producing the coated membrane, a method of increasing the selectivity of a membrane, and to a filtration / diffusion device comprising the membrane which can be used, for example, in hemodialysis applications.

The invention relates to the technical field of water bath shakers, in particular to a water bath shaker for extracting chondroitin sulfate from squids, which comprises a box body, and a motor is mounted on the outer wall of one side of the bottom end of the box body; the oscillation mechanism is arranged on the inner wall of the box body; the vibrating mechanism comprises a vibrating frame arranged in the box body, vibrating frames are fixedly connected to the outer walls of the two sides of the vibrating frame, supporting rods are fixedly connected to the outer walls of the bottom ends of the vibrating frames, eccentric wheels are fixedly connected to the outer walls of the bottom ends of the supporting rods, and mounting grooves are formed in the inner walls of the two sides of the bottom of the box body; a rotating rod is rotationally arranged on the inner wall of the mounting groove; in the oscillation process of the oscillation frame and the eccentric wheel, further oscillation treatment can be carried out, hot water distribution is more uniform through further oscillation, the oscillation extraction effect is optimized, clamping and limiting of the test tube are safer, the problems that the oscillation process is too single and the oscillation effect is poor are solved, and the oscillation extraction efficiency of the squid chondroitin sulfate is improved.

The invention relates to the technical field of functional composite probe material preparation, in particular to a three-core spinning technology-based bio-compatible gel probe and a preparation method thereof, and the three-core spinning technology-based bio-compatible gel probe comprises a composite skin layer and three functional core layers; the composite skin layer is composed of a sodium alginate-polydopamine-chitosan grafted copolymer in a mass ratio of 2: 1: 0.5, and contains # imgabs 0 # chondroitin sulfate and # imgabs 1 # polyoxyethylene; the three core layers are respectively a carbon nanotube-polyaniline # imgabs2 # hybrid material, a graphene-polypyrrole-boron nitride hybrid material and a # imgabs3 # polythiophene-hydroxyapatite hybrid material, and the mass ratio of the carbon material to the conductive polymer to the newly added nano material in each core layer is 1: 2: 0.3; and the diameter ratio of the skin layer to the three core layers is 1: 0.28: 0.22: 0.17. According to the invention, multichannel signal transmission is realized, the impedance is reduced by the hybrid material, and the conductivity is improved; the composite skin layer is matched with soft tissue mechanical properties, and interface bonding is stable; and the biocompatible component reduces inflammation, is suitable for acquiring nerve electrophysiological signals, and is easy for large-scale production.

The invention relates to the technical field of biomedical dressings, in particular to a dressing for a diabetic foot wound surface and a preparation method of the dressing. 0.2 to 0.8 part of reduced graphene oxide; 2 to 5 parts of beta-1, 3-glucan modified by chondroitin sulfate; 0.08 to 0.3 part of silver nitrate; 3 to 7 parts of N-isopropyl acrylamide-co-acrylic acid; 0.15 to 0.4 part of N, N '-methylene bisacrylamide; 2 to 5 parts of sulfhydrylated hyaluronic acid; 0.3 to 0.8 part of D-alpha-vitamin E polyethylene glycol succinate; 2 to 5 parts of amino-functionalized mesoporous silica; 1 to 3 parts of L-arginine-L-glutamate; 0.1 to 0.4 part of genipin; the super-strong mechanical property and the super-strong flexibility are realized, so that the dressing can better adapt to the irregular shape of a wound surface, and continuous protection is provided.

The invention provides glycosaminoglycan hydrogel with a physical and chemical double crosslinking mechanism as well as a preparation method and application thereof, and relates to the technical field of biological materials. The glycosaminoglycan hydrogel is prepared from the following raw materials: oxidized sodium hyaluronate, chondroitin sulfate, epsilon-polylysine hydrochloride, a polydopamine-titanium dioxide compound and a stabilizer. The glycosaminoglycan hydrogel is prepared through a physical and chemical double-crosslinking mechanism under the specific raw materials and proportion, the problems of cytotoxicity and the like caused by use of a crosslinking agent or introduction of other solvents are avoided, a stable covalent network is constructed through chemical crosslinking, the gel is endowed with the self-repairing characteristic through physical crosslinking, and the self-repairing performance of the hydrogel is improved. And the viscosity of the product is remarkably improved, so that the hydrogel has stronger mechanical support performance, is suitable for wound repair, wound healing and the like, and has good antibacterial activity and stability.

The invention discloses a protein recovery device for chondroitin sulfate production wastewater, and relates to the technical field of sewage recovery, and the technical scheme is characterized in that a separation assembly is used for separating mixed-concentration protein liquid entering the device and comprises a rotational flow layer, the rotational flow layer is used for preliminarily separating low-concentration and high-concentration protein liquid, and a U-shaped pipe and a transmission mechanism are arranged in the rotational flow layer; the device comprises an impeller arranged at the bottom of one end, located outside a rotational flow layer, of a U-shaped pipe, and a scraper located in the screening layer, and has the effects that manual interference is not needed, driving is completed through the natural effect, and under the condition that a protein screen is blocked, the blockage of the protein screen is avoided. And the impeller automatically drives the scraper to scrape and clean the inner wall of the protein screen, so that the problem of equipment blockage in the background art is solved, the frequency of manual operation is reduced, and the collaboration of the device and the working procedure efficiency of low-concentration and high-concentration protein separation and recovery are improved.

The present invention relates to the field of biomedical materials, and specifically to a three-layer composite structure collagen-based artificial skin and a preparation method thereof. The artificial skin comprises a three-layer structure, which is respectively a silicone membrane layer-collagen membrane layer-collagen sponge layer from top to bottom, wherein the thickness of the silicone membrane layer is 0.3-1 mm, the thickness of the collagen membrane layer is 0.4-10 μm, the thickness of the collagen sponge layer is 1.5-5 mm, and the total thickness is 1.8-7 mm. The main components of the porous scaffold layer of the artificial skin material are yak collagen and chondroitin sulfate, which are obtained by gradient temperature rise freeze-drying and double cross-linking treatment. The artificial skin not only has good mechanical strength, swelling performance and anti-enzymatic performance, but also has good biological activity and full-thickness skin injury wound repair effect, and has broad application prospects in the medical field.

The invention discloses starch-collagen composite hydrogel as well as a preparation method and application thereof. Collagen is catalyzed by microbial transglutaminase to form a covalent cross-linked network, and meanwhile, hydroxypropyl starch is introduced to construct a physical interpenetrating network through molecular chain penetration and hydrogen-bond interaction; and chondroitin sulfate is further integrated to strengthen the network structure and biological activity through electrostatic interaction. The method is mild in condition and does not need a toxic chemical cross-linking agent, the obtained composite hydrogel has an interpenetrating double-network structure and has excellent mechanical properties, enzymatic degradation resistance and cell affinity, the compression modulus of the composite hydrogel is remarkably improved, the composite hydrogel can keep structural stability for a long time in a collagenase environment, cell adhesion and proliferation can be effectively promoted, and the composite hydrogel has a good application prospect. The hydrogel can be widely applied to tissue engineering scaffolds, wound dressings, drug sustained-release carriers and cartilage repair materials.

The invention discloses an oral joint protection composition containing ergothioneine and a preparation method thereof, and relates to the technical field of joint protection compositions. The oral joint protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.05 to 5 parts of the ergothioneine, 20 to 50 parts of glucosamine salt, 10 to 30 parts of chondroitin sulfate, 2 to 10 parts of curcumin, 30 to 70 parts of a diluent, 1 to 10 parts of an adhesive, 0.5 to 5 parts of a lubricant, 2 to 10 parts of a disintegrating agent and 0.1 to 0.5 part of an antioxidant stabilizer. Through synergistic compatibility of active ingredients such as ergothioneine, curcumin and the like, a multi-dimensional action network of oxidation resistance, inflammation resistance and cartilage metabolism regulation is formed, the inhibition effect on joint inflammation is remarkably improved, and the limitation of a single ingredient is broken through.

The invention discloses a preparation method and application of a targeted delivery system of a metal polyphenol nano system. The invention discloses a targeted delivery system of a metal polyphenol nano system, and a preparation method of the targeted delivery system comprises the following steps: taking the metal polyphenol nano system formed based on epigallocatechin gallate and iron ions as a carrier, and loading fucoxanthin to prepare fucoxanthin nano particles with active oxygen response characteristics; then, after chondroitin sulfate capable of targeting macrophages and (5-carboxyl amyl) triphenyl phosphorus bromide capable of targeting mitochondria are coupled, the fucoxanthin delivery system with the active oxygen response characteristic and the targeting characteristic is further prepared through a double emulsion anti-solvent evaporation method. The system can realize release of fucoxanthin under stimulation of active oxygen, and realizes aggregation of a targeted delivery system in mitochondria of a specific inflammation site. The carrying system can effectively improve or relieve the inflammatory bowel disease, and the raw materials used in the carrying system are low in cost, the preparation conditions are mild, and large-scale production is easy.

Biomaterials disclosed herein can comprise a hydrogel comprising PEG, gelatin, and a glycosaminoglycan with sulfated moiety; and chondrogenic, osteogenic, and immunomodulatory cytokines; wherein the biomaterial is capable of potentiating bone regeneration in a compromised wound while reducing inflammatory response. The glycosaminoglycan with sulfated moiety can comprise heparin, heparan sulfate, keratin sulfate, chondroitin sulfate, dermatan sulfate, and / or similar materials. The biomaterial can further comprise mesenchymal stem cells (MSCs), a crosslinking initiator, microparticles and nanoparticles, and or other materials. The biomaterial can be injectable into a wound, or the biomaterial can be loaded in, or further comprise a porous scaffold providing mechanical support for other components of the biomaterial, such that it can be implanted into a wound.

The invention discloses a preparation method and application of chondroitin sulfate sodium, and belongs to the technical field of chondroitin sulfate. The preparation method of the sodium chondroitin sulfate comprises the following steps: dispersing sodium chondroitin which is not sulfated by using an organic solvent, adding a sulfonating reagent for sulfonation reaction, terminating the reaction, centrifuging, and collecting precipitate; and decoloring and drying the collected precipitate to obtain the chondroitin sulfate sodium. The average molecular weight of the final product obtained by the method is controllable, the 6-site overall sulfonation rate is up to 90% or above, the 6-site independent sulfonation rate is up to 60%-85%, the quality of products among batches is stable, and sodium chondroitin sulfate based on the characteristics is more suitable for human body absorption, high in bioavailability and higher in safety and effectiveness; moreover, the method can realize abundant sources, uniform structure, stable quality, mild reaction and reasonable and controllable production cycle without complex extraction and purification processes, and is suitable for large-scale commercial production and application.

The invention belongs to the technical field of biology, and particularly relates to a compound for treating myocardial infarction. The chondroitin sulfate oligosaccharide is extracted from natural chondroitin sulfate, and after the chondroitin sulfate oligosaccharide is treated into an oligosaccharide chain through a chemical enzyme method, experiments prove that the chondroitin sulfate oligosaccharide is beneficial to the survival of myocardial cells. Compared with other products with similar functions, the glycopeptide polymer hydrogel formed after introduction of the polymer skeleton overcomes the problem of batch-to-batch difference of natural products and is clear in structure, and the hydrogel has excellent biological activity and does not need to entrap other active ingredients such as drugs. The effects of supporting the ventricular wall of the infarction region and promoting the survival of myocardial cells are obvious, the myocardial infarction area can be effectively limited, the thickness of the ventricular wall of the infarction region is increased, the damage of myocardial infarction to the heart is reduced, and the application prospect is very good.

The invention belongs to the field of external application, and particularly relates to a preparation method of glucosamine chondroitin cream for relieving discomfort of bones, muscles and ligaments, which comprises the following steps: collecting preparation node characteristic indexes of linear preparation nodes and characteristic indexes of purification liquid and centrifugal tubes of purification equipment of the preparation nodes at all levels, and dividing the linear preparation nodes into preposed and continuous preparation nodes; a purification result of the front purification equipment of the front preparation node is input into the continuous purification equipment of the continuous preparation node to serve as a purification raw material; according to the concentration information of the pre-purification equipment and the continuous purification equipment, the purification intermediate waste amount of a linear preparation node, the natural oxidation raw material amount and the corresponding glucosamine chondroitin cream increment are calculated by combining the characteristic indexes of the preparation node and the characteristic indexes of the purification liquid and the centrifugal tube; and the purification equipment of the linear preparation node is scheduled, so that the purification equipment runs in an optimized state. The method reasonably reduces purification intermediate wastes, optimizes the purification liquid, effectively fuses products in an environment range, and brings great economic benefits to enterprises.

The invention relates to the technical field of aquaculture, in particular to a leiocassis longirostris intestinal health functional additive based on spermaceti soxhlet metabolite. The Leiocassis longirostris intestinal health functional additive comprises the following functional components: chondroitin sulfate, N-acetyl-D-galactosamine, 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4'- The composition is prepared from the following raw materials: 4, 6-dihydroxyflavone, polydatin, ascorbic acid, glucoside, arabinogalactan, 3-O-beta-D-galactopyranosyl-L-arabinose, tyramine, 4A-hydroxytetrahydrobiopterin, hyaluronic acid and 3-mercapto-2-butanol. According to the technical scheme, the technical problem that an existing probiotic viable bacterium preparation is difficult to meet the intestinal health management requirement in intensive culture of the leiocassis longirostris can be solved. Compared with the prior art, the scheme is more efficient, stable and safe, healthy development of the Leiocassis longirostris breeding industry can be effectively promoted, and the method has ideal application and popularization prospects.

The invention relates to the technical field of nutritional supplements, and discloses a pet nutritional supplement capable of improving immunity, which is prepared from the following raw materials in parts by weight: 35 to 45 parts of taurine, 5 to 8 parts of natural lutein, 10 to 30 parts of chondroitin sulfate, 10 to 20 parts of water, 20 to 30 parts of corn starch, 10 to 20 parts of semen plantaginis and 30 to 50 parts of microbial fermentation products. The 35-45 parts of taurine and the 10-30 parts of chondroitin sulfate can be selected from one or more of sardine powder, black and white fish powder, beef powder, pig powder, chicken powder and the like. According to the pet nutritional supplement capable of improving immunity and the preparation method thereof, the semen plantaginis is added into the raw materials, so that the pet kidney can be promoted to generate more urine, urethra flushing is facilitated, bacterium breeding is reduced, and urinary system infection is prevented, and the taurine is added, so that heart condition improvement, brain development promotion and retina protection are facilitated. When the pet supplement is prepared, the pet supplement is prepared by using a freeze-drying machine, so that the loss of nutrient substances is reduced.

The invention discloses Escherichia coli with high fructose chondroitin yield and a production method of the Escherichia coli, and belongs to the technical field of microorganisms. On the basis of Escherichia coli K4, a coding gene of a key enzyme in a fructose chondroitin synthesis route is mutated, and a strain for improving the fructose chondroitin synthesis capability is constructed. The yield of fructose chondroitin in a 5 L fermentation tank reaches 10 g / L, the yield of fructose chondroitin in a 100 L fermentation tank reaches 15-20 g / L, the obtained final product is uniform in structure and stable in quality, the production cost is effectively reduced, the environmental pollution risk is reduced, and the strain is suitable for large-scale industrial production and application.

The invention discloses an antibacterial dressing for promoting wound repair and regeneration and a preparation method thereof, and the preparation method comprises the following steps: adding hydrogen peroxide into a silk fibroin aqueous solution to prepare a silk fibroin aqueous solution premixed with hydrogen peroxide; horse radish peroxidase is added into the dopamine modified sodium alginate solution, and the dopamine modified sodium alginate solution premixed with horse radish peroxidase is prepared. Preparing a chondroitin sulfate composite solution; uniformly mixing the silk fibroin aqueous solution premixed with hydrogen peroxide, the dopamine modified sodium alginate solution premixed with horse radish peroxidase and the chondroitin sulfate composite solution. Hydrogel formed by cross-linking silk fibroin and chondroitin sulfate is used as a main body material of the dressing, and meanwhile, dopamine modified sodium alginate is introduced, so that the dressing has the photo-thermal antibacterial characteristic; magnesium oxide microspheres are introduced as a pore-foaming agent, micropores are formed in the dressing, and the liquid absorption property and the air permeability of the dressing are improved; the umbilical cord mesenchymal stem cell exosome is introduced, so that angiogenesis at the wound is promoted, and the wound healing promoting capability of the dressing is improved.

Disclosed in the present invention is a method for preparing a biomimetic biomaterial, which method comprises using heterologous or homologous corneal stroma or skin as a template, perfusing a chondroitin sulfate derivative into the interspace of the corneal stroma or skin, allowing the chondroitin sulfate derivative to cure and then digesting the original corneal stroma or skin, and thereby obtaining a polymer backbone having copied structural characteristics of a natural stroma. A biomimetic corneal stroma having the structural characteristics of a natural cornea or a biomimetic skin is obtained by means of refilling with collagen. The preparation method provided in the present invention removes heterologous proteins from a natural biological tissue and reduces the immunogenicity while maintaining the structural characteristics of the natural tissue, thereby realizing the biomimetics of an artificial biomaterial on a natural biological tissue structure.

The invention belongs to the field of medicine, health and health, and particularly relates to a preparation method for controllably synthesizing polysulfonic acid mucopolysaccharide with different molecular weights, which comprises the following steps: reaction, acid-base neutralization, formamide removal by alcohol precipitation, desalting and freeze-drying. Wherein the reaction process comprises the following steps: adding sodium chondroitin sulfate and a pyridine sulfur trioxide complex into a mixed solvent of N, N-dimethylformamide and dimethyl sulfoxide, and heating for reaction to obtain a reaction mixture; the volume fraction of the dimethyl sulfoxide in the mixed solvent is 0-80%. According to the method, the low-price pyridine sulfur trioxide complex (about 150 yuan / 500g) is used, and the ratio of N, N-dimethylformamide to dimethyl sulfoxide in the mixed solvent is adjusted, so that the solubility of the chondroitin sulfate sodium and the pyridine sulfur trioxide complex is changed, and the polysulfonic acid mucopolysaccharide with molecular weights in different ranges can be regulated and generated.