349 results about "Chondroitin" patented technology

The invention provides glycosaminoglycan hydrogel with a physical and chemical double crosslinking mechanism as well as a preparation method and application thereof, and relates to the technical field of biological materials. The glycosaminoglycan hydrogel is prepared from the following raw materials: oxidized sodium hyaluronate, chondroitin sulfate, epsilon-polylysine hydrochloride, a polydopamine-titanium dioxide compound and a stabilizer. The glycosaminoglycan hydrogel is prepared through a physical and chemical double-crosslinking mechanism under the specific raw materials and proportion, the problems of cytotoxicity and the like caused by use of a crosslinking agent or introduction of other solvents are avoided, a stable covalent network is constructed through chemical crosslinking, the gel is endowed with the self-repairing characteristic through physical crosslinking, and the self-repairing performance of the hydrogel is improved. And the viscosity of the product is remarkably improved, so that the hydrogel has stronger mechanical support performance, is suitable for wound repair, wound healing and the like, and has good antibacterial activity and stability.

The invention discloses starch-collagen composite hydrogel as well as a preparation method and application thereof. Collagen is catalyzed by microbial transglutaminase to form a covalent cross-linked network, and meanwhile, hydroxypropyl starch is introduced to construct a physical interpenetrating network through molecular chain penetration and hydrogen-bond interaction; and chondroitin sulfate is further integrated to strengthen the network structure and biological activity through electrostatic interaction. The method is mild in condition and does not need a toxic chemical cross-linking agent, the obtained composite hydrogel has an interpenetrating double-network structure and has excellent mechanical properties, enzymatic degradation resistance and cell affinity, the compression modulus of the composite hydrogel is remarkably improved, the composite hydrogel can keep structural stability for a long time in a collagenase environment, cell adhesion and proliferation can be effectively promoted, and the composite hydrogel has a good application prospect. The hydrogel can be widely applied to tissue engineering scaffolds, wound dressings, drug sustained-release carriers and cartilage repair materials.

The invention discloses an oral joint protection composition containing ergothioneine and a preparation method thereof, and relates to the technical field of joint protection compositions. The oral joint protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.05 to 5 parts of the ergothioneine, 20 to 50 parts of glucosamine salt, 10 to 30 parts of chondroitin sulfate, 2 to 10 parts of curcumin, 30 to 70 parts of a diluent, 1 to 10 parts of an adhesive, 0.5 to 5 parts of a lubricant, 2 to 10 parts of a disintegrating agent and 0.1 to 0.5 part of an antioxidant stabilizer. Through synergistic compatibility of active ingredients such as ergothioneine, curcumin and the like, a multi-dimensional action network of oxidation resistance, inflammation resistance and cartilage metabolism regulation is formed, the inhibition effect on joint inflammation is remarkably improved, and the limitation of a single ingredient is broken through.

Biomaterials disclosed herein can comprise a hydrogel comprising PEG, gelatin, and a glycosaminoglycan with sulfated moiety; and chondrogenic, osteogenic, and immunomodulatory cytokines; wherein the biomaterial is capable of potentiating bone regeneration in a compromised wound while reducing inflammatory response. The glycosaminoglycan with sulfated moiety can comprise heparin, heparan sulfate, keratin sulfate, chondroitin sulfate, dermatan sulfate, and / or similar materials. The biomaterial can further comprise mesenchymal stem cells (MSCs), a crosslinking initiator, microparticles and nanoparticles, and or other materials. The biomaterial can be injectable into a wound, or the biomaterial can be loaded in, or further comprise a porous scaffold providing mechanical support for other components of the biomaterial, such that it can be implanted into a wound.

The invention relates to the technical field of aquaculture, in particular to a leiocassis longirostris intestinal health functional additive based on spermaceti soxhlet metabolite. The Leiocassis longirostris intestinal health functional additive comprises the following functional components: chondroitin sulfate, N-acetyl-D-galactosamine, 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4'- The composition is prepared from the following raw materials: 4, 6-dihydroxyflavone, polydatin, ascorbic acid, glucoside, arabinogalactan, 3-O-beta-D-galactopyranosyl-L-arabinose, tyramine, 4A-hydroxytetrahydrobiopterin, hyaluronic acid and 3-mercapto-2-butanol. According to the technical scheme, the technical problem that an existing probiotic viable bacterium preparation is difficult to meet the intestinal health management requirement in intensive culture of the leiocassis longirostris can be solved. Compared with the prior art, the scheme is more efficient, stable and safe, healthy development of the Leiocassis longirostris breeding industry can be effectively promoted, and the method has ideal application and popularization prospects.

The invention discloses an antibacterial dressing for promoting wound repair and regeneration and a preparation method thereof, and the preparation method comprises the following steps: adding hydrogen peroxide into a silk fibroin aqueous solution to prepare a silk fibroin aqueous solution premixed with hydrogen peroxide; horse radish peroxidase is added into the dopamine modified sodium alginate solution, and the dopamine modified sodium alginate solution premixed with horse radish peroxidase is prepared. Preparing a chondroitin sulfate composite solution; uniformly mixing the silk fibroin aqueous solution premixed with hydrogen peroxide, the dopamine modified sodium alginate solution premixed with horse radish peroxidase and the chondroitin sulfate composite solution. Hydrogel formed by cross-linking silk fibroin and chondroitin sulfate is used as a main body material of the dressing, and meanwhile, dopamine modified sodium alginate is introduced, so that the dressing has the photo-thermal antibacterial characteristic; magnesium oxide microspheres are introduced as a pore-foaming agent, micropores are formed in the dressing, and the liquid absorption property and the air permeability of the dressing are improved; the umbilical cord mesenchymal stem cell exosome is introduced, so that angiogenesis at the wound is promoted, and the wound healing promoting capability of the dressing is improved.

The invention belongs to the field of medicine, health and health, and particularly relates to a preparation method for controllably synthesizing polysulfonic acid mucopolysaccharide with different molecular weights, which comprises the following steps: reaction, acid-base neutralization, formamide removal by alcohol precipitation, desalting and freeze-drying. Wherein the reaction process comprises the following steps: adding sodium chondroitin sulfate and a pyridine sulfur trioxide complex into a mixed solvent of N, N-dimethylformamide and dimethyl sulfoxide, and heating for reaction to obtain a reaction mixture; the volume fraction of the dimethyl sulfoxide in the mixed solvent is 0-80%. According to the method, the low-price pyridine sulfur trioxide complex (about 150 yuan / 500g) is used, and the ratio of N, N-dimethylformamide to dimethyl sulfoxide in the mixed solvent is adjusted, so that the solubility of the chondroitin sulfate sodium and the pyridine sulfur trioxide complex is changed, and the polysulfonic acid mucopolysaccharide with molecular weights in different ranges can be regulated and generated.

The invention discloses hyaluronic acid endo-lyase and application thereof in preparation of hyaluronic acid with specific molecular weight. The hyaluronate lyase can cut a beta1-4 glucosidic bond to generate unsaturated oligosaccharide, has the characteristics of high enzyme activity and catalytic efficiency and good stability, and the enzymolysis reaction can be carried out in water without adding metal ions and a buffer solution, so that the hyaluronate lyase has the advantages of simplicity in product recovery and no need of removing salt. The hyaluronate lyase not only can be used for degrading hyaluronic acid, but also can be used for degrading chondroitin sulfate, not only can be used for preparing polysaccharide with specific molecular weight, but also can be used for completely degrading the polysaccharide into unsaturated disaccharide, and has a wide application prospect in the fields of cosmetics, food, health care products, medicines and the like.

The present invention relates to a cartilage tissue single cell extraction kit and an extraction method thereof, the kit comprises: (1) a first enzyme for enzymatic hydrolysis, the first enzyme for enzymatic hydrolysis comprising one or more of trypsin, chondroitinase ABC and fucosidase; (2) a second enzyme for enzymolysis, wherein the second enzyme for enzymolysis comprises collagenase II, neutral protease II, hyaluronidase and Dnase I; and (3) a basic solution, wherein the basic solution comprises a basic culture medium and / or a buffer solution. The invention also provides a method for extracting single cells from cartilage tissues by using the kit. The cartilage tissue single-cell suspension prepared by the method has the advantages of high cell yield, high activity and the like, and has a wide application prospect.

The invention discloses a pH response hydrogel microneedle, which comprises a microneedle substrate, microneedle tips are arranged on the microneedle substrate, the height of the microneedle tips is 100-1600 microns, the diameter of the tip ends of the microneedle tips is 5-15 microns, and the density of the microneedle tips is 200-1000 pieces per square centimeter. The preparation method comprises the following steps: preparing a hyaluronic acid solution for the microneedle substrate, preparing a methacrylated chondroitin sulfate solution for the microneedle tip, preparing methacrylated orthoester, preparing orthoester, preparing a non-steroidal anti-inflammatory drug solution, preparing a microneedle tip solution, preparing the microneedle and demolding the microneedle to prepare the complete soluble microneedle patch. The invention has the advantages of high drug efficiency, high efficiency in dissolving fat / water drugs, and long-acting slow release.

The invention belongs to the field of bioactive protein peptide materials, and provides an animal-derived composite fermented skin-tendering protein peptide and a preparation method thereof. According to the invention, bovine-derived collagen and fish-derived protein are subjected to synergistic fermentation to prepare a protein peptide core, and the outer surface of the protein peptide core is coated with an edible gelatin and chondroitin sulfate composite layer; the excellent performance that a small molecule peptide core with the molecular weight being 500-1500 Da, a stable coating layer with the coating rate being 80-99%, the glycine-proline-hydroxyproline sequence collagen peptide fragment accounting for 30-60% and the pH responsive swelling ratio being 1.5-3.0 are achieved, the technical problems that traditional protein peptide is poor in stability and low in bioavailability are solved, and the protein peptide has wide application value in the field of skin care products.

The invention provides chondroitin sulfate excision enzyme and application of the chondroitin sulfate excision enzyme in dermatan sulfate preparation. The amino acid sequence of the chondroitin sulfate excision enzyme is shown as SEQ ID NO: 3, and the chondroitin sulfate excision enzyme has broad-spectrum substrate specificity and can efficiently degrade hyaluronic acid and glycosaminoglycans such as chondroitin sulfate (A, C, E and D). The chondroitin sulfate excision enzyme can efficiently act on a substrate by virtue of a unique catalytic mechanism, so that efficient preparation of oligosaccharide and low-molecular-weight polysaccharide is realized, and the chondroitin sulfate excision enzyme can be particularly used for enzymatic preparation of dermatan sulfate. The chondroitin sulfate excision enzyme has efficient catalytic performance and unique treatment advantages, not only fills the technical blank in related fields, but also opens up a wide space for the development of carbohydrate chemicals in the fields of biological medicines, cosmetics, foods and the like.

The invention belongs to the technical field of hydrogel dressings, and particularly relates to a high-viscosity antibacterial hydrogel dressing as well as a preparation method and application thereof. The high-viscosity antibacterial hydrogel dressing is prepared from the following raw materials in parts by weight: 4 to 15 parts of oxidized sodium hyaluronate, 2 to 10 parts of chondroitin sulfate, 2.5 to 5 parts of epsilon-polylysine hydrochloride, 1 to 5 parts of hydroxypropyl methyl cellulose and 2 to 4 parts of sodium alginate. The hydrogel dressing prepared by the invention has good effects of inhibiting bacteria, stopping bleeding and promoting wound healing.

The invention belongs to the field of biomolecule detection, and discloses a polysaccharide high-throughput detection method and application. The method can be used for detecting the heparin precursor, a sample does not need to be purified, the heparin precursor in a complex sample can be directly recognized and degraded by enzyme specificity to generate disaccharide, and then the content of the disaccharide of the heparin precursor is detected through high-throughput EchoMS, so that the heparin precursor is subjected to rapid, high-throughput, qualitative and quantitative analysis; the method is used for screening and evaluating elements influencing heparin precursor synthesis, screening and evaluating heparin precursor synthesis strains, optimizing the heparin precursor fermentation process and the like. The method can also be used for high-throughput detection of glycosaminoglycans such as hyaluronic acid, heparin and chondroitin sulfate and other polysaccharides such as pullulan, curdlan, xanthan gum and glucan, and can be used for structural analysis of polysaccharides.

The utility model discloses a concentration device, which belongs to the technical field of chondroitin sulfate production and processing equipment and comprises a protective shell used for preventing heat from overflowing when chondroitin sulfate is concentrated, a concentration barrel used for concentration of chondroitin sulfate is arranged in the protective shell, the top of the concentration barrel is connected with a cross rod, and the cross rod is connected with the protective shell. A cover plate is installed on the side face of the transverse rod, a first motor is installed at the top of the cover plate, an output shaft of the first motor is connected with a rotating shaft through a coupler, a connecting column and paddles are installed on the periphery of the rotating shaft, two defoaming blocks are symmetrically welded to the periphery of the connecting column, and corrugated grooves are formed in the side faces of the two defoaming blocks. The side faces of the two defoaming blocks are both connected with springs, the ends, away from the defoaming blocks, of the springs are connected with defoaming plates, and the concentration device can improve the defoaming efficiency, the evaporation efficiency and the product quality.

The invention belongs to the technical field of chondroitin sulfate production, and particularly relates to an informatization control management method and system for chondroitin sulfate production. The method comprises the following steps: performing risk assessment on a chondroitin sulfate production process to determine an initial abnormal point, defining a screening time period based on the initial abnormal point to determine a supplementary abnormal point, forming an abnormal point set, classifying the abnormal points into a continuous abnormal interval or an interval abnormal interval according to the time interval of the abnormal points in the abnormal point set, and when the operation of the production equipment enters the predicted abnormal time period or reaches the predicted abnormal time node, generating early warning prompt information. According to the method, the accuracy of anomaly recognition is improved by integrating initial and supplementary anomaly points, the accuracy of risk pre-judgment is improved by performing classification prediction on different anomaly types by using a trend model, and the intelligent level of control management is improved while the production stability is guaranteed through an active grading early warning mechanism.

A collagen-boosting supplement that contains at least 4, optionally at least 10, collagen building block amino acids and is substantially free of histidine. The building block amino acids may be selected from: Glycine, L-Proline, L-Hydroxyproline, L-Glutamic Acid, L-Alanine, L-Arginine, L-Aspartic Acid, L-Serine, L-Leucine, L-Lysine, L-Hydroxylysine, L-Valine, L-Phenylalanine, L-Threonine, L-Isoleucine, L-Methionine, and L-Tyrosine. The supplement may further comprise: vitamin C; one or more mast cell stabilisers which may be selected from: quercetin, molybdenum, bromelain, DAO (diamine oxidase), Urtica Dioica (stinging nettle), Nigella Sativa (black cumin), Zingiber (ginger), Scutellaria baicalensis (Chinese skullcap), Sambucus (Elderberry), Aloe Vera, Syzygium aromaticum (clove), and matcha; one or more collagen-strengthening herbs which may be selected from: Bilberry, Centella, and Astragulus; one or more Glucosaminoglycans (GAGs) which may be selected from: Hyaluronic acid, glucosamine, and chondroitin; one or more antioxidants which may be selected from: astaxanthin and resveratrol; and / or one or more cofactors which may be selected from: zinc, biotin, silicon, manganese, magnesium, Vitamin D, Vitamin A, Vitamin E, and iron. The supplement may be a foodstuff, medicament, nutraceutical, or cosmetic. The supplement may be used to treat hypermobile Ehlers-Danlos syndrome (hEDS) while reducing the likelihood of mast cell degranulation through histamine activation.

The application belongs to the technical field of chondroitin sulfate preparation, and particularly relates to application and application method of liquid sulfur dioxide in preparation of chondroitin sulfate or chondroitin sulfate salt. In the application, liquid sulfur dioxide is used as a solvent to perform sulfonation modification treatment on unsulfated chondroitin, on the one hand, the use of traditional high-boiling organic reagents is avoided, the difficulty of three-waste treatment is reduced, and the process is more green and environmentally friendly; on the other hand, the post-treatment operation of the sulfonation reaction is simpler, and sulfur dioxide can be removed from the system by only temperature control or pressure adjustment, so that solvent residues possibly existing in the final product are avoided, and the product is safer. In addition, by adjusting the key conditions of the sulfonation reaction, the proportion of chondroitin sulfate with 6 independent sulfation in the disaccharide unit of the sulfonation product is 70% to 90%, the reaction is stable and reliable, and the sulfonation rate is high.

The invention relates to the field of food deep processing, and particularly discloses a processing technology of sodium chondroitin sulfate, which comprises the following steps: S1, crushing raw materials, boiling, carrying out alkaline hydrolysis, and carrying out enzymolysis to obtain an enzymatic hydrolysate; s2, the enzymatic hydrolysate is subjected to filtration, ultrafiltration, oxidation, secondary filtration, ultrafiltration membrane filtration, alcohol precipitation, dehydration, centrifugation, drying, crushing and sieving, and a finished product is obtained; the method has the advantages of high extraction rate, high extraction rate and high purity.

The invention relates to the technical field of functional hydrogel materials, in particular to ultrasonic cavitation self-adaptive lubricating hydrogel as well as a preparation method and application thereof. According to the ultrasonic cavitation self-adaptive lubricating hydrogel provided by the invention, at least one of N-acryloyl glycinamide, N-acryloyl acrylamide and methylacryloyl chitosan is used as a monomer; at least one of sodium hyaluronate methacrylate, hyaluronic acid-polyethylene glycol double-grafted copolymer and methacrylated chondroitin sulfate is used as a lubricant, persulfate is used as an oxidizing agent, molybdenum disulfide and / or MXene are / is used as a reducing agent, the dosage ratio of all the raw materials is controlled, the characteristics of non-invasiveness and deep penetrability of ultrasonic waves can be utilized, and the high-purity chondroitin sulfate can be obtained. In-vivo controllable efficient in-situ polymerization is realized, surgical implantation wounds are avoided, the fitness of the material and tissues is improved, and an in-vivo complex microenvironment is adapted; and the raw materials are high in biocompatibility and high in biological safety.

The application discloses a kind of postharvest fruit and vegetable chlorosis delaying composite preservative and method, the effective component of the composite preservative includes the concentration of 10-60ppm chondroitin sulfate, the concentration of 10-60ppm heparin and the concentration of 10-60ppm hyaluronic acid.The application uses chondroitin sulfate, heparin and hyaluronic acid as composite preservative to treat postharvest citron, and shows synergistic effect of delaying chlorophyll content reduction of citron, inhibiting fruit chlorosis, so as to effectively delay postharvest citron shelf life, and the composite preservative of the application has the characteristics of green and efficient, effective components can be completely water-soluble, and environmental pollution cannot be generated.

The invention discloses a chondroitin sulfate oligosaccharide derivative and a preparation method and application thereof.The chondroitin sulfate oligosaccharide derivative is shown in the formula (I), the definition of substituent groups in the formula is shown in the specification, and the derivative comprises di-, tetra-and hexasaccharide derivatives of chondroitin sulfate B and T, has endogenous anticoagulation and anti-inflammatory activity, and can be used for preparing the chondroitin sulfate oligosaccharide derivative. The compound can be developed into anticoagulant and antithrombotic drugs.

The present application relates to a kind of self-setting composite bone implants based on gallic acid complex salt and preparation method thereof, belong to bone repair material field.The present application is specifically related to a kind of self-setting composite bone implants, it is characterized in that, component A, component B and component C, the component A is gallic acid and the complex salt of at least two metal elements, wherein the at least two metal elements are the metal element of promoting bone tissue regeneration or the trace metal element required by human body, and the at least two metal elements include calcium, the component B is the complex of calcium / strontium chondroitin sulfate and calcium sulfate, and the component C is magnesium calcium hydrogen phosphate complex.The self-setting composite bone implant of the present application has the characteristics of antibacterial, anti-inflammatory, promoting osteogenesis, instant shaping and all degradation, can meet the clinical needs of bone tumor, bone injury and the like, has wide application prospect in various bone repair, reconstruction and replacement.

The invention relates to the technical field of medical biological materials, and discloses a mussel mucin-chondroitin sulfate composite hydrogel and a preparation method and application thereof, and the preparation method comprises the following steps: respectively preparing a mussel mucin aqueous solution and a chondroitin sulfate aqueous solution; the mass volume concentration of the mussel mucoprotein aqueous solution is 1%-10%, the mass volume concentration of the chondroitin sulfate aqueous solution is 1%-10%, the mass ratio of the mussel mucoprotein to the chondroitin sulfate is 3: 1-1: 3, the two solutions are mixed and stirred or oscillated under the conditions that the temperature is 4-37 DEG C and the pH value is 7.0-9.0, a cross-linking reaction is conducted, and hydrogel is formed; the cross-linking mode is physical cross-linking, chemical cross-linking or physical-chemical dual cross-linking; according to the invention, excellent tissue adhesion, injectability, self-healing property and biodegradability are realized, cell adhesion and proliferation can be effectively promoted, and the gel is suitable for various medical beauty scenes such as skin filling, wound repairing and anti-aging nursing.

The invention relates to the technical field of fertilizers, in particular to a nutrient polymerized polyglutamic acid compound fertilizer and a preparation method thereof, and the nutrient polymerized polyglutamic acid compound fertilizer comprises the following raw materials in parts by weight: 6-10 parts of gamma-polyglutamic acid, 25-30 parts of filler, 20-25 parts of a nitrogen source, 12-15 parts of a phosphorus source, 10-14 parts of a potassium source, 4-6 parts of a compound additive and 0.1-0.2 part of a fungicide. In the preparation of the granules, the humic acid powder is coated by a first treating agent prepared from the soluble starch, the salicylic acid, the sodium alginate and the like, so that the activity of the humic acid is effectively protected, and meanwhile, the mechanical strength and the slow-release characteristic of the granules are further enhanced by a second treating agent prepared from the components such as the chitosan and the fulvic acid through a chelation cross-linking effect; in addition, chondroitin sulfate, calcium nitrate, ammonium molybdate and molasses in the batch jointly provide medium trace elements and organic carbon sources, and synchronous release and efficient utilization of nutrients after the fertilizer is applied to soil are ensured.

The invention belongs to the field of bone nutrition powder, and particularly relates to bone nutrition powder for assisting in relieving arthritis pain and a preparation process. In the preparation, the herba eupatorii extract and the kelp extract are matched to effectively improve the arthritis pain relieving effect of the preparation, and the lophatherum gracile, the raspberry, the motherwort and the purslane are adopted to further improve the arthritis pain relieving effect of the fermented product through the processes of fermentation and the like; the preparation is applied to preparation of the bone nutrition powder, and the bone nutrition powder for assisting in relieving arthritis pain is prepared from the bone nutrition powder, protein, fat, water-soluble dietary fibers, vitamins, mineral elements and chondroitin sulfate. The obtained bone nutrition powder can effectively provide nutrition, also has an excellent health care effect, assists in relieving arthritis pain, and is excellent in performance and relatively good in application prospect.

The invention discloses a construction method of a high-activity mutant of chondroitin 6-O sulfotransferase, and belongs to the field of biological enzyme engineering and synthetic biology. Interaction sites of MusC6 protein and a substrate PAPS are accurately analyzed through a computer-aided molecular docking technology, and key active sites are directionally modified by adopting a strategy of combining rational design and saturated mutation. Experimental results show that the catalytic performance of the obtained mutant S139T / S140L / Y364M / K412V is remarkably improved, and the enzyme activity of the mutant reaches 1182U / L and is improved by 4.4 times compared with the enzyme activity of a wild type 270U / L. According to the invention, the technical bottleneck of low catalytic efficiency of a wild type enzyme on a natural substrate is solved through a multi-site synergistic mutation strategy, and a biocatalyst with industrial application value is provided for efficiently synthesizing chondroitin sulfate C by a microbiological method.

The application discloses starch-based colon double-targeting microgels, a preparation method and application thereof. The preparation method comprises the following steps: covalently connecting chondroitin sulfate to beta-cyclodextrin by EDC to prepare beta-cyclodextrin-chondroitin sulfate; encapsulating a hydrophobic bioactive substance in the hydrophobic cavity of the beta-cyclodextrin in the beta-cyclodextrin-chondroitin sulfate to prepare beta-cyclodextrin-chondroitin sulfate loaded with the bioactive substance; modifying dextrin by octenyl succinic anhydride to prepare modified dextrin; and making a first mixed reaction system comprising the beta-cyclodextrin-chondroitin sulfate loaded with the bioactive substance, the modified dextrin and sodium trimetaphosphate to have a crosslinking reaction to prepare starch-based colon double-targeting microgels. The starch-based colon double-targeting microgels have good encapsulation and protection effects on the bioactive substance, and have a targeting effect in the colon, so that the bioactive substance can be more effectively delivered.

The utility model relates to the technical field of chondroitin processing, in particular to an anti-clogging chondroitin trickling filtration device, which can effectively improve the filtering effect on water, is convenient for taking out a trickling filtration component in the device, enables the trickling filtration component to be cleaned more comprehensively and thoroughly, and prevents the device from being clogged due to incomplete cleaning for a long time. The normal use of the device is ensured; comprising a barrel and a confluence disc, three sets of supporting legs are fixedly arranged at the bottom end of the barrel in an annular array mode, a barrel cover is arranged on a cover in threaded connection with the top end of the barrel, a baffle ring is fixedly arranged on the outer wall of the barrel, and the baffle ring and the barrel are integrally formed; the bottom ends of the biochemical balls abut against the top end of the lower-layer water-passing partition plate, an upper-layer water-passing partition plate is placed at the top ends of the biochemical balls, a ceramic ring is placed at the top end of the upper-layer water-passing partition plate, filter cotton is placed at the top end of the ceramic ring, and filter cloth is placed at the top end of the filter cotton.

Described herein are targeted ChABC fusion proteins, complexes thereof, and uses thereof. The targeted ChABC fusion proteins can include a ChABC polypeptide that can be linked to a Gal-3 polypeptide. Monomer targeted ChABC fusion proteins can form homogeneous or heterogeneous complexes. The targeted ChABC fusion proteins and complexes thereof can be formulated as pharmaceutical formulations. The targeted ChABC fusion proteins, complexes thereof, and formulations thereof can be administered to a subject in need thereof.