491 results about "Chondroitin" patented technology

The invention relates to an oral mucosa repairing medicine and a preparation method thereof, and belongs to the technical field of medicines, the medicine comprises barnacle peptide, salivary lactobacillus, resveratrol, a biphasic adhesion matrix, hydroxyapatite, vitamin B family and other components. The preparation method comprises the following steps: sequentially carrying out enzymolysis on barnacle gooseneck by pepsin and lumbrukinase to obtain barnacle peptide. The double-phase adhesion matrix comprises a water-phase matrix, an oil-phase matrix and lecithin; the water-phase matrix contains a chondroitin sulfate-chitosan quaternary ammonium salt compound, sodium alginate and other components; the oil-phase matrix comprises sesame oil, beeswax, a polylactic acid-glycolic acid copolymer and the like. According to the invention, barnacle peptide and salivary lactobacillus are prepared by adopting a special method and are matched with resveratrol for use, so that multi-target and multi-path promotion of oral mucosa repair can be realized; the chondroitin sulfate-chitosan quaternary ammonium salt compound is prepared through electrostatic and physical embedding, the chondroitin sulfate-chitosan quaternary ammonium salt compound is matched with other components to prepare the biphasic adhesion matrix, the biocompatibility is good, the adhesion is high, and the action time of a sustained-release drug is well prolonged.

The invention relates to a low-molecular-weight chondroitin sulfate composition as well as a preparation method and application thereof, and aims to solve the problems that a chondroitin sulfate product obtained by a single degradation mode is limited in function and difficult to synergistically regulate intestinal barriers. The preparation method comprises the following steps: firstly, preparing LMCSO through oxidative degradation; secondly, preparing LMCSH through hydrothermal degradation; and finally, the LMCSD is prepared through deamination degradation. Finally, the obtained LMCSO, LMCSH and LMCSD are compounded according to the weight ratio of 5: 3: 2, and the low-molecular-weight chondroitin sulfate composition LMCSs-C is obtained. The composition LMCSs-C prepared by the invention can synergistically improve the intestinal barrier function through multiple action mechanisms, not only shows an obvious intestinal barrier repair effect in vitro and animal models, but also has good biocompatibility and safety, and shows a wide application prospect in intestinal health maintenance and related disease intervention.

The invention relates to the technical field of biomedicine, and discloses a human metapneumovirus culture method which comprises the following steps: a culture medium is prepared and put into a culture bottle, and the culture medium comprises a DMEM culture medium, glucose, sodium bicarbonate, an HEPES buffer solution, chondroitin sulfate and hyaluronic acid; host cells are selected; preparing a culture solution, wherein the culture solution comprises L-glutamine, bovine serum albumin, antibiotics and N-acetylcysteine; and inoculating the human metapneumovirus into the culture medium for adsorption and infection. The chondroitin sulfate and the hyaluronic acid are added into the culture medium, the chondroitin sulfate and the hyaluronic acid can be combined with glycosaminoglycans on the surfaces of the host cells to form a compound, interaction sites of viruses and the host cells are increased, then virus receptor expression on the surfaces of the host cells is enhanced, the cell membrane environment is optimized, and therefore the anti-tumor effect is achieved. Therefore, the attachment and entry efficiency of the hMPV is improved, and the rapid proliferation of the virus is obviously promoted.

The invention relates to the technical field of functional composite probe material preparation, in particular to a three-core spinning technology-based bio-compatible gel probe and a preparation method thereof, and the three-core spinning technology-based bio-compatible gel probe comprises a composite skin layer and three functional core layers; the composite skin layer is composed of a sodium alginate-polydopamine-chitosan grafted copolymer in a mass ratio of 2: 1: 0.5, and contains # imgabs 0 # chondroitin sulfate and # imgabs 1 # polyoxyethylene; the three core layers are respectively a carbon nanotube-polyaniline # imgabs2 # hybrid material, a graphene-polypyrrole-boron nitride hybrid material and a # imgabs3 # polythiophene-hydroxyapatite hybrid material, and the mass ratio of the carbon material to the conductive polymer to the newly added nano material in each core layer is 1: 2: 0.3; and the diameter ratio of the skin layer to the three core layers is 1: 0.28: 0.22: 0.17. According to the invention, multichannel signal transmission is realized, the impedance is reduced by the hybrid material, and the conductivity is improved; the composite skin layer is matched with soft tissue mechanical properties, and interface bonding is stable; and the biocompatible component reduces inflammation, is suitable for acquiring nerve electrophysiological signals, and is easy for large-scale production.

The invention relates to the technical field of biomedical dressings, in particular to a dressing for a diabetic foot wound surface and a preparation method of the dressing. 0.2 to 0.8 part of reduced graphene oxide; 2 to 5 parts of beta-1, 3-glucan modified by chondroitin sulfate; 0.08 to 0.3 part of silver nitrate; 3 to 7 parts of N-isopropyl acrylamide-co-acrylic acid; 0.15 to 0.4 part of N, N '-methylene bisacrylamide; 2 to 5 parts of sulfhydrylated hyaluronic acid; 0.3 to 0.8 part of D-alpha-vitamin E polyethylene glycol succinate; 2 to 5 parts of amino-functionalized mesoporous silica; 1 to 3 parts of L-arginine-L-glutamate; 0.1 to 0.4 part of genipin; the super-strong mechanical property and the super-strong flexibility are realized, so that the dressing can better adapt to the irregular shape of a wound surface, and continuous protection is provided.

The invention provides glycosaminoglycan hydrogel with a physical and chemical double crosslinking mechanism as well as a preparation method and application thereof, and relates to the technical field of biological materials. The glycosaminoglycan hydrogel is prepared from the following raw materials: oxidized sodium hyaluronate, chondroitin sulfate, epsilon-polylysine hydrochloride, a polydopamine-titanium dioxide compound and a stabilizer. The glycosaminoglycan hydrogel is prepared through a physical and chemical double-crosslinking mechanism under the specific raw materials and proportion, the problems of cytotoxicity and the like caused by use of a crosslinking agent or introduction of other solvents are avoided, a stable covalent network is constructed through chemical crosslinking, the gel is endowed with the self-repairing characteristic through physical crosslinking, and the self-repairing performance of the hydrogel is improved. And the viscosity of the product is remarkably improved, so that the hydrogel has stronger mechanical support performance, is suitable for wound repair, wound healing and the like, and has good antibacterial activity and stability.

The invention discloses a protein recovery device for chondroitin sulfate production wastewater, and relates to the technical field of sewage recovery, and the technical scheme is characterized in that a separation assembly is used for separating mixed-concentration protein liquid entering the device and comprises a rotational flow layer, the rotational flow layer is used for preliminarily separating low-concentration and high-concentration protein liquid, and a U-shaped pipe and a transmission mechanism are arranged in the rotational flow layer; the device comprises an impeller arranged at the bottom of one end, located outside a rotational flow layer, of a U-shaped pipe, and a scraper located in the screening layer, and has the effects that manual interference is not needed, driving is completed through the natural effect, and under the condition that a protein screen is blocked, the blockage of the protein screen is avoided. And the impeller automatically drives the scraper to scrape and clean the inner wall of the protein screen, so that the problem of equipment blockage in the background art is solved, the frequency of manual operation is reduced, and the collaboration of the device and the working procedure efficiency of low-concentration and high-concentration protein separation and recovery are improved.

The invention discloses starch-collagen composite hydrogel as well as a preparation method and application thereof. Collagen is catalyzed by microbial transglutaminase to form a covalent cross-linked network, and meanwhile, hydroxypropyl starch is introduced to construct a physical interpenetrating network through molecular chain penetration and hydrogen-bond interaction; and chondroitin sulfate is further integrated to strengthen the network structure and biological activity through electrostatic interaction. The method is mild in condition and does not need a toxic chemical cross-linking agent, the obtained composite hydrogel has an interpenetrating double-network structure and has excellent mechanical properties, enzymatic degradation resistance and cell affinity, the compression modulus of the composite hydrogel is remarkably improved, the composite hydrogel can keep structural stability for a long time in a collagenase environment, cell adhesion and proliferation can be effectively promoted, and the composite hydrogel has a good application prospect. The hydrogel can be widely applied to tissue engineering scaffolds, wound dressings, drug sustained-release carriers and cartilage repair materials.

The invention discloses an oral joint protection composition containing ergothioneine and a preparation method thereof, and relates to the technical field of joint protection compositions. The oral joint protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.05 to 5 parts of the ergothioneine, 20 to 50 parts of glucosamine salt, 10 to 30 parts of chondroitin sulfate, 2 to 10 parts of curcumin, 30 to 70 parts of a diluent, 1 to 10 parts of an adhesive, 0.5 to 5 parts of a lubricant, 2 to 10 parts of a disintegrating agent and 0.1 to 0.5 part of an antioxidant stabilizer. Through synergistic compatibility of active ingredients such as ergothioneine, curcumin and the like, a multi-dimensional action network of oxidation resistance, inflammation resistance and cartilage metabolism regulation is formed, the inhibition effect on joint inflammation is remarkably improved, and the limitation of a single ingredient is broken through.

The invention discloses a preparation method and application of a targeted delivery system of a metal polyphenol nano system. The invention discloses a targeted delivery system of a metal polyphenol nano system, and a preparation method of the targeted delivery system comprises the following steps: taking the metal polyphenol nano system formed based on epigallocatechin gallate and iron ions as a carrier, and loading fucoxanthin to prepare fucoxanthin nano particles with active oxygen response characteristics; then, after chondroitin sulfate capable of targeting macrophages and (5-carboxyl amyl) triphenyl phosphorus bromide capable of targeting mitochondria are coupled, the fucoxanthin delivery system with the active oxygen response characteristic and the targeting characteristic is further prepared through a double emulsion anti-solvent evaporation method. The system can realize release of fucoxanthin under stimulation of active oxygen, and realizes aggregation of a targeted delivery system in mitochondria of a specific inflammation site. The carrying system can effectively improve or relieve the inflammatory bowel disease, and the raw materials used in the carrying system are low in cost, the preparation conditions are mild, and large-scale production is easy.

Biomaterials disclosed herein can comprise a hydrogel comprising PEG, gelatin, and a glycosaminoglycan with sulfated moiety; and chondrogenic, osteogenic, and immunomodulatory cytokines; wherein the biomaterial is capable of potentiating bone regeneration in a compromised wound while reducing inflammatory response. The glycosaminoglycan with sulfated moiety can comprise heparin, heparan sulfate, keratin sulfate, chondroitin sulfate, dermatan sulfate, and / or similar materials. The biomaterial can further comprise mesenchymal stem cells (MSCs), a crosslinking initiator, microparticles and nanoparticles, and or other materials. The biomaterial can be injectable into a wound, or the biomaterial can be loaded in, or further comprise a porous scaffold providing mechanical support for other components of the biomaterial, such that it can be implanted into a wound.

The invention discloses a preparation method and application of chondroitin sulfate sodium, and belongs to the technical field of chondroitin sulfate. The preparation method of the sodium chondroitin sulfate comprises the following steps: dispersing sodium chondroitin which is not sulfated by using an organic solvent, adding a sulfonating reagent for sulfonation reaction, terminating the reaction, centrifuging, and collecting precipitate; and decoloring and drying the collected precipitate to obtain the chondroitin sulfate sodium. The average molecular weight of the final product obtained by the method is controllable, the 6-site overall sulfonation rate is up to 90% or above, the 6-site independent sulfonation rate is up to 60%-85%, the quality of products among batches is stable, and sodium chondroitin sulfate based on the characteristics is more suitable for human body absorption, high in bioavailability and higher in safety and effectiveness; moreover, the method can realize abundant sources, uniform structure, stable quality, mild reaction and reasonable and controllable production cycle without complex extraction and purification processes, and is suitable for large-scale commercial production and application.

The invention relates to the technical field of aquaculture, in particular to a leiocassis longirostris intestinal health functional additive based on spermaceti soxhlet metabolite. The Leiocassis longirostris intestinal health functional additive comprises the following functional components: chondroitin sulfate, N-acetyl-D-galactosamine, 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4'- The composition is prepared from the following raw materials: 4, 6-dihydroxyflavone, polydatin, ascorbic acid, glucoside, arabinogalactan, 3-O-beta-D-galactopyranosyl-L-arabinose, tyramine, 4A-hydroxytetrahydrobiopterin, hyaluronic acid and 3-mercapto-2-butanol. According to the technical scheme, the technical problem that an existing probiotic viable bacterium preparation is difficult to meet the intestinal health management requirement in intensive culture of the leiocassis longirostris can be solved. Compared with the prior art, the scheme is more efficient, stable and safe, healthy development of the Leiocassis longirostris breeding industry can be effectively promoted, and the method has ideal application and popularization prospects.

The invention discloses an antibacterial dressing for promoting wound repair and regeneration and a preparation method thereof, and the preparation method comprises the following steps: adding hydrogen peroxide into a silk fibroin aqueous solution to prepare a silk fibroin aqueous solution premixed with hydrogen peroxide; horse radish peroxidase is added into the dopamine modified sodium alginate solution, and the dopamine modified sodium alginate solution premixed with horse radish peroxidase is prepared. Preparing a chondroitin sulfate composite solution; uniformly mixing the silk fibroin aqueous solution premixed with hydrogen peroxide, the dopamine modified sodium alginate solution premixed with horse radish peroxidase and the chondroitin sulfate composite solution. Hydrogel formed by cross-linking silk fibroin and chondroitin sulfate is used as a main body material of the dressing, and meanwhile, dopamine modified sodium alginate is introduced, so that the dressing has the photo-thermal antibacterial characteristic; magnesium oxide microspheres are introduced as a pore-foaming agent, micropores are formed in the dressing, and the liquid absorption property and the air permeability of the dressing are improved; the umbilical cord mesenchymal stem cell exosome is introduced, so that angiogenesis at the wound is promoted, and the wound healing promoting capability of the dressing is improved.

The invention belongs to the field of medicine, health and health, and particularly relates to a preparation method for controllably synthesizing polysulfonic acid mucopolysaccharide with different molecular weights, which comprises the following steps: reaction, acid-base neutralization, formamide removal by alcohol precipitation, desalting and freeze-drying. Wherein the reaction process comprises the following steps: adding sodium chondroitin sulfate and a pyridine sulfur trioxide complex into a mixed solvent of N, N-dimethylformamide and dimethyl sulfoxide, and heating for reaction to obtain a reaction mixture; the volume fraction of the dimethyl sulfoxide in the mixed solvent is 0-80%. According to the method, the low-price pyridine sulfur trioxide complex (about 150 yuan / 500g) is used, and the ratio of N, N-dimethylformamide to dimethyl sulfoxide in the mixed solvent is adjusted, so that the solubility of the chondroitin sulfate sodium and the pyridine sulfur trioxide complex is changed, and the polysulfonic acid mucopolysaccharide with molecular weights in different ranges can be regulated and generated.

The invention discloses hyaluronic acid endo-lyase and application thereof in preparation of hyaluronic acid with specific molecular weight. The hyaluronate lyase can cut a beta1-4 glucosidic bond to generate unsaturated oligosaccharide, has the characteristics of high enzyme activity and catalytic efficiency and good stability, and the enzymolysis reaction can be carried out in water without adding metal ions and a buffer solution, so that the hyaluronate lyase has the advantages of simplicity in product recovery and no need of removing salt. The hyaluronate lyase not only can be used for degrading hyaluronic acid, but also can be used for degrading chondroitin sulfate, not only can be used for preparing polysaccharide with specific molecular weight, but also can be used for completely degrading the polysaccharide into unsaturated disaccharide, and has a wide application prospect in the fields of cosmetics, food, health care products, medicines and the like.

The present invention relates to a cartilage tissue single cell extraction kit and an extraction method thereof, the kit comprises: (1) a first enzyme for enzymatic hydrolysis, the first enzyme for enzymatic hydrolysis comprising one or more of trypsin, chondroitinase ABC and fucosidase; (2) a second enzyme for enzymolysis, wherein the second enzyme for enzymolysis comprises collagenase II, neutral protease II, hyaluronidase and Dnase I; and (3) a basic solution, wherein the basic solution comprises a basic culture medium and / or a buffer solution. The invention also provides a method for extracting single cells from cartilage tissues by using the kit. The cartilage tissue single-cell suspension prepared by the method has the advantages of high cell yield, high activity and the like, and has a wide application prospect.

The invention discloses a preparation method and application of a targeted carcinoembryonic chondroitin sulfate positive cell engineered extracellular vesicle, and belongs to the technical field of biomedicine. The invention provides a targeting carcinoembryonic chondroitin sulfate positive cell engineered extracellular vesicle. The targeting carcinoembryonic chondroitin sulfate positive cell engineered extracellular vesicle is obtained by collecting stable overexpression cell supernatant containing ofCSbps protein and separating; the sequence of the ofCSbps is selected from one or more combined polypeptide sequences in SEQ ID NO.1-7, or a derivative sequence taking one polypeptide sequence in SEQ ID NO.1-7 as a skeleton or a derivative sequence taking more combined polypeptide sequences as the skeleton. The engineered extracellular vesicle disclosed by the invention has the characteristic of targeting an ofCS positive cell. As the extracellular vesicles have nanoscale lipid inclusion structures, the extracellular vesicles are suitable for fundamental research and clinical diagnosis and treatment of various diseases such as human and animal placenta-derived physiology / pathology, tumors / cancers and the like.

The invention discloses a pH response hydrogel microneedle, which comprises a microneedle substrate, microneedle tips are arranged on the microneedle substrate, the height of the microneedle tips is 100-1600 microns, the diameter of the tip ends of the microneedle tips is 5-15 microns, and the density of the microneedle tips is 200-1000 pieces per square centimeter. The preparation method comprises the following steps: preparing a hyaluronic acid solution for the microneedle substrate, preparing a methacrylated chondroitin sulfate solution for the microneedle tip, preparing methacrylated orthoester, preparing orthoester, preparing a non-steroidal anti-inflammatory drug solution, preparing a microneedle tip solution, preparing the microneedle and demolding the microneedle to prepare the complete soluble microneedle patch. The invention has the advantages of high drug efficiency, high efficiency in dissolving fat / water drugs, and long-acting slow release.

The invention belongs to the field of bioactive protein peptide materials, and provides an animal-derived composite fermented skin-tendering protein peptide and a preparation method thereof. According to the invention, bovine-derived collagen and fish-derived protein are subjected to synergistic fermentation to prepare a protein peptide core, and the outer surface of the protein peptide core is coated with an edible gelatin and chondroitin sulfate composite layer; the excellent performance that a small molecule peptide core with the molecular weight being 500-1500 Da, a stable coating layer with the coating rate being 80-99%, the glycine-proline-hydroxyproline sequence collagen peptide fragment accounting for 30-60% and the pH responsive swelling ratio being 1.5-3.0 are achieved, the technical problems that traditional protein peptide is poor in stability and low in bioavailability are solved, and the protein peptide has wide application value in the field of skin care products.

The invention provides chondroitin sulfate excision enzyme and application of the chondroitin sulfate excision enzyme in dermatan sulfate preparation. The amino acid sequence of the chondroitin sulfate excision enzyme is shown as SEQ ID NO: 3, and the chondroitin sulfate excision enzyme has broad-spectrum substrate specificity and can efficiently degrade hyaluronic acid and glycosaminoglycans such as chondroitin sulfate (A, C, E and D). The chondroitin sulfate excision enzyme can efficiently act on a substrate by virtue of a unique catalytic mechanism, so that efficient preparation of oligosaccharide and low-molecular-weight polysaccharide is realized, and the chondroitin sulfate excision enzyme can be particularly used for enzymatic preparation of dermatan sulfate. The chondroitin sulfate excision enzyme has efficient catalytic performance and unique treatment advantages, not only fills the technical blank in related fields, but also opens up a wide space for the development of carbohydrate chemicals in the fields of biological medicines, cosmetics, foods and the like.

The invention discloses a lung airway organoid culture system with a trachea branch morphological structure as well as application and a culture method of the lung airway organoid culture system, and relates to the technical field of lung airway organoid culture. The hydrogel culture system is prepared from I-type rat tail collagen, gelatin, hyaluronic acid, laminin, low-melting agarose, chondroitin sulfate, fibronectin, elastin, epidermal growth factors, fibronectin L and transglutaminase; the induction culture medium is prepared from a 1XB27 supplement, a fiber growth factor, glutamine, glutathione, A8301, SB202190, IBMX, 8-Br-cAMP, FGF7, FGF10, Shh and ascorbic acid. The culture system is clear in component, low in price and cost and simple and convenient in preparation method, more importantly, the lung airway organoid which is closer to the physiological state can be efficiently induced to be formed within a short time, and the generated organoid has a branch form similar to an in-vivo trachea and contains various types of functional airway epithelial cells at the same time; and a more preferable scheme is provided for constructing an airway organ with an airway branch form based on the adult lung stem cells.

The invention belongs to the technical field of hydrogel dressings, and particularly relates to a high-viscosity antibacterial hydrogel dressing as well as a preparation method and application thereof. The high-viscosity antibacterial hydrogel dressing is prepared from the following raw materials in parts by weight: 4 to 15 parts of oxidized sodium hyaluronate, 2 to 10 parts of chondroitin sulfate, 2.5 to 5 parts of epsilon-polylysine hydrochloride, 1 to 5 parts of hydroxypropyl methyl cellulose and 2 to 4 parts of sodium alginate. The hydrogel dressing prepared by the invention has good effects of inhibiting bacteria, stopping bleeding and promoting wound healing.

The invention discloses NMN-loaded Gel-OCS hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: step 1, oxidizing chondroitin sulfate by using an oxidizing agent to obtain oxidized chondroitin sulfate OCS; and 2, dissolving the OCS prepared in the step 1 and borax in water, uniformly mixing to obtain an OCS precursor solution, dissolving gelatin in water, fully dissolving to obtain a Gel precursor solution, dissolving NMN in water to obtain an NMN mother solution, mixing the Gel precursor solution with the NMN mother solution in proportion, mixing with the OCS precursor solution to prepare a hydrogel precursor solution, and incubating to obtain the NMN-loaded Gel-OCS hydrogel. The method for preparing the hydrogel is simple, wide in raw material source, low in cost and suitable for large-scale production and application.

The invention provides a preparation method of fish gelatin polysaccharide from fish gelatin, which is characterized in that fish maw is subjected to degreasing, proteolysis, alcohol precipitation and column separation to obtain the fish gelatin polysaccharide. According to the application, the crushed cod gelatin is taken as a raw material, the fish gelatin polysaccharide is prepared from the crushed cod gelatin through a specific enzymolysis process in combination with graded purification technologies such as alcohol precipitation, chromatographic column separation and dialysis, glycosaminoglycan in the prepared fish gelatin polysaccharide contains chondroitin sulfate which accounts for 90% of glycosaminoglycan 7, and the repairing effect on the wound of the zebra fish is obvious.

The invention belongs to the field of biomolecule detection, and discloses a polysaccharide high-throughput detection method and application. The method can be used for detecting the heparin precursor, a sample does not need to be purified, the heparin precursor in a complex sample can be directly recognized and degraded by enzyme specificity to generate disaccharide, and then the content of the disaccharide of the heparin precursor is detected through high-throughput EchoMS, so that the heparin precursor is subjected to rapid, high-throughput, qualitative and quantitative analysis; the method is used for screening and evaluating elements influencing heparin precursor synthesis, screening and evaluating heparin precursor synthesis strains, optimizing the heparin precursor fermentation process and the like. The method can also be used for high-throughput detection of glycosaminoglycans such as hyaluronic acid, heparin and chondroitin sulfate and other polysaccharides such as pullulan, curdlan, xanthan gum and glucan, and can be used for structural analysis of polysaccharides.

The invention discloses a corneal endothelial cell protection liquid for reducing corneal endothelial cell metabolism, which takes dextran or a combination of dextran and chondroitin sulfate as a dehydrating agent, takes beta-hydroxybutyric acid and dexmedetomidine as antioxidants, and simultaneously contains two buffer systems of sodium bicarbonate and HEPES. According to the invention, the metabolism of corneal endothelial cells can be reduced, and the form of the corneal endothelial cells in vitro can be better maintained.

The utility model discloses a concentration device, which belongs to the technical field of chondroitin sulfate production and processing equipment and comprises a protective shell used for preventing heat from overflowing when chondroitin sulfate is concentrated, a concentration barrel used for concentration of chondroitin sulfate is arranged in the protective shell, the top of the concentration barrel is connected with a cross rod, and the cross rod is connected with the protective shell. A cover plate is installed on the side face of the transverse rod, a first motor is installed at the top of the cover plate, an output shaft of the first motor is connected with a rotating shaft through a coupler, a connecting column and paddles are installed on the periphery of the rotating shaft, two defoaming blocks are symmetrically welded to the periphery of the connecting column, and corrugated grooves are formed in the side faces of the two defoaming blocks. The side faces of the two defoaming blocks are both connected with springs, the ends, away from the defoaming blocks, of the springs are connected with defoaming plates, and the concentration device can improve the defoaming efficiency, the evaporation efficiency and the product quality.

The present invention relates to a low-molecular-weight chondroitin sulfate composition, its preparation method, and application, aiming to address the problem that chondroitin sulfate products obtained by a single degradation method have limited functions and are difficult to synergistically regulate the intestinal barrier. The preparation method first prepares LMCSO by oxidative degradation; secondly, prepares LMCSH by hydrothermal degradation; and finally, prepares LMCSD by deamination degradation. Finally, the obtained LMCSO, LMCSH, and LMCSD are compounded in a weight ratio of 5:3:2 to obtain a low-molecular-weight chondroitin sulfate composition LMCSs-C. The composition LMCSs-C prepared by the present invention can synergistically improve intestinal barrier function through multiple mechanisms of action. It not only demonstrates significant intestinal barrier repair effects in vitro and in animal models, but also has good biocompatibility and safety, showing broad application prospects in maintaining intestinal health and intervening in related diseases.

The present invention discloses a method for increasing the chondroitin sulfate content in chicken meat. By utilizing the metabolic and conversion effects of chondroitin sulfate in chickens, chondroitin sulfate is added to chicken feed at an addition amount of 0.25% - 2%, and the chickens are fed for 30 days. This can significantly increase the chondroitin sulfate content in chicken meat, obtain functional chicken meat rich in chondroitin sulfate, especially increase the chondroitin sulfate content in chicken breast and chicken leg meat, enhance the nutritional value of chicken meat, and also improve the economic value.