Preparation method of hyaluronic acid/gelatin/chondroitin sulfate bone repair bionic scaffold

Abstract

The invention discloses a preparation method of hyaluronic acid / gelatin / chondroitin sulfate bone repair bionic scaffold. The method comprises the steps of adding activator and furfurylamine into MES buffer solution of hyaluronic acid to obtain modified hyaluronic acid solid; adding EDC / NHS (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide / N-hydroxysuccinimide) into gelatin aqueous solution, and adding furancarboxylic acid to obtain modified gelatin solid; dissolving the above two solids into MES buffer solution to obtain a mixed solution; dissolving MAL-PEG-MAL (maleimide-polyethylene glycol-maleimide) into MES buffer solution, adding into the mixed solution, and reacting in 37 DEG C water bath to form transparent cross-linked hydrogel; soaking the hydrogel in MES buffer solution of sodium chondroitin sulfate, adding EDC / NHS, and reacting under stirring to obtain the cartilage repair bionic scaffold. The bionic scaffold has the advantages of interpenetrating network structure, excellent biocompatibility and bioactivity, better compression strength, anti-washout property and degradability, simple preparation process, and easy operation.

Description

technical field [0001] The invention belongs to the field of bioactive materials, and relates to cartilage tissue repair, filling and tissue engineering repair materials, in particular to a preparation method of a hyaluronic acid / gelatin / chondroitin sulfate bone repair bionic scaffold. Background technique [0002] Articular cartilage is a relatively special tissue in the human body. It has no blood vessels, nerves and lymphocytes, so its self-repair ability is very poor. At present, the traditional clinical treatment methods mainly include microfracture, subchondral bone drilling, subchondral grinding, autologous cartilage transplantation and autologous cell transplantation. secondary trauma. At present, there is no method that can successfully replace the damaged site and achieve the purpose of cartilage regeneration. [0003] Cartilage tissue engineering is the hottest research method at present. It combines cells, environmental factors and scaffold materials to simula...

AI Technical Summary

Problems solved by technology

Synthetic polymer hydrogels have excellent mechanical properties, but their application in cartilage repair is limited due to the lack of cell binding sites and biocompatibility; for natural biomaterials, it is generally difficult to form a hydrogel system, and there is a degradation rate Shortcomings such as speed and mechanical properties can not meet the demand

Composite natural materials with synthetic materials can improve the biocompatibility of the material while improving the mechanical properties, but it is far from the natural cartilage extracellular matrix

There is also a method of preparing hydrogel repair materials by modifying and modifying collagen, but collagen has a large molecular weight and is not easy to bond with other substances, except that hexafluoroisopropanol is almost insoluble in any solvent, while hexafluoroisopropanol Propanol is highly toxic and not suitable for use in the field of biomedical materials

Therefore, it is very difficult to obtain a collagen solution, and it is even more difficult to modify and modify collagen, which makes it unsuitable as a hydrogel material.

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