Preparation method of hyaluronic acid/gelatin/chondroitin sulfate bone repair bionic scaffold

A technology of hyaluronic acid and sulfated cartilage, applied in medical science, prosthesis, etc., can solve the problems of large molecular weight of collagen, fast degradation rate, difficulty in collagen modification and modification, etc., and achieve short gelation time, The effect of mild reaction conditions and high selectivity

Inactive Publication Date: 2012-11-14
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Synthetic polymer hydrogels have excellent mechanical properties, but their application in cartilage repair is limited due to the lack of cell binding sites and biocompatibility; for natural biomaterials, it is generally difficult to form a hydrogel system, and there is a degradation rate Shortcomings such as speed and mechanical properties can not meet the demand
Composite natural materials with synthetic materials can improve the biocompatibility of the material while improving the mechanical properties, but it is far from the natural cartilage extrac

Method used

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  • Preparation method of hyaluronic acid/gelatin/chondroitin sulfate bone repair bionic scaffold
  • Preparation method of hyaluronic acid/gelatin/chondroitin sulfate bone repair bionic scaffold
  • Preparation method of hyaluronic acid/gelatin/chondroitin sulfate bone repair bionic scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Add 0.654g 4-(4, 6-Dimethoxytriazin-2-yl)-4-methylmorpholine hydrochloride (abbreviation: DMTMM), stirred for 0.5h, then added 220μL furfurylamine dropwise, reacted for 24h, dialyzed for 3 days, freeze-dried Obtain modified hyaluronic acid solid;

[0034] (2) Add 400mg of carbodiimide (abbreviation: EDC) and 267mg of N-hydroxysuccinimide (abbreviation: NHS) to 100ml of gelatin aqueous solution with a concentration of 0.5%. After stirring evenly, add 125mg of furanic acid and react for 24 hours. Dialyzed for 3 days, freeze-dried to obtain modified gelatin solid;

[0035] (3) Dissolve 30 mg of the modified hyaluronic acid solid obtained in step (1) and 45 mg of the modified gelatin solid obtained in step (2) in MES buffer to obtain a mixed solution, and the volume concentration of the two is 1% respectively and 1.5%;

[0036] (4) Dissolve 37.5 mg of double-terminated maleimide polyvinyl alcohol (abbreviation: MAL-PEG-MAL) in MES buffer to obtain a MAL-PEG-MAL solut...

Embodiment 2

[0041] (1) Add 1.38g DMTMM to 0.4% hyaluronic acid MES buffer (0.5g hyaluronic acid mass), stir for 1h, then add 220μL furfurylamine dropwise, react for 36h, dialyze for 4 days, Freeze-drying to obtain a modified hyaluronic acid solid;

[0042] (2) Add 800 mg of EDC and 533 mg of NHS to a gelatin aqueous solution with a concentration of 1%, stir evenly, add 500 mg of furanic acid, react for 36 hours, dialyze for 4 days, freeze-dry to obtain a modified gelatin solid;

[0043] (3) Dissolve 45 mg of the modified hyaluronic acid solid obtained in step (1) and 105 mg of the modified gelatin solid obtained in step (2) in 3 ml of MES buffer to obtain a mixed solution. The volume concentrations of the two are 1.5% and 3.5%;

[0044] (4) Dissolve 75 mg of MAL-PEG-MAL in MES buffer to obtain a MAL-PEG-MAL solution;

[0045] (5) Add the MAL-PEG-MAL solution obtained in step (4) to the mixed solution obtained in step (3), stir evenly, and perform a click chemical reaction in a water bat...

Embodiment 3

[0050] (1) Add 2.02g DMTMM to 0.8% hyaluronic acid MES buffer (0.5g hyaluronic acid mass), stir for 1h, then add 220μL furfurylamine dropwise, react for 48h, dialyze for 5 days, Freeze-drying to obtain a modified hyaluronic acid solid;

[0051] (2) Add 1.2g of EDC and 0.8g of NHS to the gelatin aqueous solution with a concentration of 1.5%, stir evenly, add 1.1g of furanic acid, react for 48 hours, dialyze for 5 days, freeze-dry to obtain the modified gelatin solid;

[0052] (3) Dissolve 60 mg of the modified hyaluronic acid solid obtained in step (1) and 165 mg of the modified gelatin solid obtained in step (2) in MES to obtain a mixed solution, and the volume concentrations of the two are 2% and 5.5% respectively %;

[0053] (4) Dissolve 150mg of MAL-PEG-MAL in MES buffer to obtain MAL-PEG-MAL solution;

[0054] (5) Add the MAL-PEG-MAL solution obtained in step (4) to the mixed solution obtained in step (3), stir evenly, and perform a click chemical reaction in a water bat...

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Abstract

The invention discloses a preparation method of hyaluronic acid/gelatin/chondroitin sulfate bone repair bionic scaffold. The method comprises the steps of adding activator and furfurylamine into MES buffer solution of hyaluronic acid to obtain modified hyaluronic acid solid; adding EDC/NHS (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide) into gelatin aqueous solution, and adding furancarboxylic acid to obtain modified gelatin solid; dissolving the above two solids into MES buffer solution to obtain a mixed solution; dissolving MAL-PEG-MAL (maleimide-polyethylene glycol-maleimide) into MES buffer solution, adding into the mixed solution, and reacting in 37 DEG C water bath to form transparent cross-linked hydrogel; soaking the hydrogel in MES buffer solution of sodium chondroitin sulfate, adding EDC/NHS, and reacting under stirring to obtain the cartilage repair bionic scaffold. The bionic scaffold has the advantages of interpenetrating network structure, excellent biocompatibility and bioactivity, better compression strength, anti-washout property and degradability, simple preparation process, and easy operation.

Description

technical field [0001] The invention belongs to the field of bioactive materials, and relates to cartilage tissue repair, filling and tissue engineering repair materials, in particular to a preparation method of a hyaluronic acid / gelatin / chondroitin sulfate bone repair bionic scaffold. Background technique [0002] Articular cartilage is a relatively special tissue in the human body. It has no blood vessels, nerves and lymphocytes, so its self-repair ability is very poor. At present, the traditional clinical treatment methods mainly include microfracture, subchondral bone drilling, subchondral grinding, autologous cartilage transplantation and autologous cell transplantation. secondary trauma. At present, there is no method that can successfully replace the damaged site and achieve the purpose of cartilage regeneration. [0003] Cartilage tissue engineering is the hottest research method at present. It combines cells, environmental factors and scaffold materials to simula...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/20A61L27/22
Inventor 陈晓峰余凤曹晓东曾蕾张青
Owner SOUTH CHINA UNIV OF TECH
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