119 results about "Large molecular weight" patented technology

The invention belongs to the technical field of cosmetics, and particularly relates to an EGCG-DNA sodium composition with anti-oxidation and anti-aging effects and application of the EGCG-DNA sodium composition. The EGCG-DNA sodium composition is prepared from the following components in parts by weight: 0.5 to 2 parts of EGCG-DNA inclusion, and 0.5 to 2 parts of PDRN (Protein Deoxyribose Nucleic Acid) from rose. An EGCG-DNA sodium inclusion is wrapped by adopting a specific nano wrapping technology, the problems that DNA sodium is large in molecular weight and impermeable and EGCG is unstable are solved, then the rose-sourced DNA sodium is matched with one another and has a synergistic effect in a preferable dosage range, free radicals are effectively resisted, and the skin aging problem is solved.

The invention discloses an efficient extraction method of large-molecular-weight astragalus polysaccharide, which belongs to the technical field of plant extraction, and comprises the following steps: cleaning astragalus, filtering, and crushing; the preparation method comprises the following steps: mixing a beta-1, 3-glucanase aqueous solution and trehalose ethylene diamine, and stirring for 10-30 minutes to obtain an enzyme protection system; soaking the radix astragali powder in a citric acid solution, adding an enzyme protection system, applying a bipolar pulsed electric field, and reacting at 40-60 DEG C for 30-40 minutes; adjusting the concentration of the ethanol to 40-50%, standing, centrifuging and collecting crude astragalus polysaccharide; purifying, decolorizing, concentrating, freeze-drying and crushing the crude astragalus polysaccharide to obtain refined astragalus polysaccharide; the mass ratio of the trehalose ethylene diamine to the beta-1, 3-glucanase is (15: 1) to (30: 1). The enzyme protection system maintains the activity of the enzyme, the enzyme and the pulsed electric field act synergistically, the extraction time is shortened, and the extraction rate is increased while the astragalus polysaccharide with large molecular weight is obtained.

The invention discloses a silk fibroin solution injection capable of forming gel in situ and a preparation method and application thereof.The preparation method comprises the following steps that a regenerated silk fibroin solution with the molecular weight of 100 kDa-250 kDa and a hydrogen bond breaker are evenly mixed to obtain a mixed solution, the mixed solution is subjected to quick-freezing supercooling crystallization and vacuum drying to obtain silk fibroin powder, and the silk fibroin powder is dried to obtain the silk fibroin solution injection capable of forming gel in situ. And sterilizing and dissolving in normal saline to obtain the silk fibroin solution injection. A hydrogen bond breaker is introduced into a high-molecular-weight regenerated silk fibroin solution, a beta folding structure between silk fibroin molecules is inhibited, damage to the silk fibroin molecules in the freeze-drying process is reduced through a quick-freezing and supercooling crystallization method, and aggregation of the silk fibroin molecules is further reduced; after redissolving, an injection is formed and injected into local tissues, the micromolecular hydrogen bond breaker quickly diffuses, and the large molecular weight regenerated silk fibroin recovers hydrogen bond interaction and then forms hydrogel in situ through physical crosslinking, so that in-vivo in-situ gelling of the silk fibroin is realized.

The invention relates to preparation and application of a desoxyribonucleic acid and desoxyribonucleic acid derivative transdermal delivery system, and belongs to the technical field of biological medicine and skin care products. Desoxyribonucleic acid and derivatives thereof have the effects of resisting aging, repairing and the like, but are difficult to penetrate through a skin barrier due to large molecular weight and strong polarity. According to the invention, hydroxyethyl chitosan, desoxyribonucleic acid and derivatives thereof are combined through electrostatic interaction to form the nano-composite. The compound is small in particle size and can promote transdermal absorption through hair follicle or cuticle gaps. The preparation method is simple, does not need an auxiliary reagent, and comprises the step of mixing a hydroxyethyl chitosan solution and a deoxyribonucleic acid derivative solution. Experiments show that the compound significantly enhances the transdermal efficiency, and has the effects of promoting cell repair and proliferation and resisting inflammation. The composition can be used for skin care products, and is high in safety and wide in application prospect.

The invention discloses an auxiliary open fire heating device for improving the surface quality of extruded plastics. The auxiliary open fire heating device comprises a bracket base device and a fire jetting combustion furnace tray device and is characterized in that the bracket base device comprises a base and a support frame, and the support frame is mounted on the base; the fire jetting combustion furnace tray device comprises a gas inlet valve, a gas guide pipe and nozzle devices; the gas inlet valve is mounted at an inlet of the gas guide pipe; the nozzle devices are mounted on the gas guide pipe; and the fire jetting combustion furnace tray device is mounted on the bracket base device through the support frame. The auxiliary open fire heating device has the advantages of simplicity, convenience and feasibility and also has the advantages of being convenient to use, high in efficiency, high in practicability, light in structure and convenient to mount, disassemble and operate. Generally, when large-molecular-weight plastics of a molecular chain net structure, such as organosilane crosslinked polyethylene, are processed and produced, the auxiliary open fire heating device is directly mounted on a nose of the existing extruder by using magnetic absorption and utilizes open fire to carry out auxiliary open fire heating on a forming die orifice and an extruded wire so as to improve the poor surface quality of the extruded product.

The invention discloses high-heat-resistance low-dielectric-loss resin as well as a preparation method and application thereof, and relates to the technical field of resin materials. The high-heat-resistance and low-dielectric-loss resin is prepared through the curing reaction of the alicyclic epoxy resin and the active ester; the core of the method is that alicyclic epoxy resin is subjected to curing reaction with active ester by utilizing the characteristics of small molecular weight, high crosslinking density and no glyceryl ether structure, so that the defects of large molecular weight, low crosslinking density, low Tg, low cost and the like of resin formed by bisphenol A type epoxy resin, phenolic aldehyde type epoxy resin and the active ester in the prior art are effectively overcome. And electrical defects caused by a glyceryl ether-containing structure of part of resin are overcome. The prepared high-heat-resistance low-dielectric-loss resin has high heat resistance and excellent electrical performance, the manufacturing process is simple and easy to operate, and the high-heat-resistance low-dielectric-loss resin can be applied to the electronic field of copper-clad plates and the like and has wide application prospects.

The invention relates to the field of macromolecules, and discloses a polymer as well as a preparation method and application thereof. The polymer has a chemical structural unit as shown in a formula 1. Compared with polyester, the polymer disclosed by the invention has the characteristics of higher degradation rate in a water environment, large molecular weight and good heat resistance or mechanical property, and can be applied to the fields of degradable packaging materials, drug controlled release carriers and the like.

The invention provides polyimide and a preparation method thereof. The preparation method comprises the following steps: adding a sodium hydroxide aqueous solution into a monomer solution containing a dianhydride monomer and an aprotic solvent, mixing, adding diisocyanate, carrying out a polymerization reaction to obtain a reaction product system, and precipitating from the reaction product system to obtain polyimide, wherein the feeding time of the diisocyanate is 4 to 5 hours; in the monomer solution, the molar concentration of the dianhydride monomer is 0.5-0.6 mol / L; the obtained polyimide has the advantages of high specific concentration viscosity, high molecular weight and excellent high temperature resistance.

The invention discloses waterborne acrylic resin and a preparation method thereof, and belongs to the technical field of acrylic resin processing. Comprising the following components in parts by mass: 44-55 parts of deionized water, 0.5-3 parts of a carboxyl-containing monomer, 0-17 parts of a hydroxyl-containing monomer, 5-35 parts of an acrylate monomer, 5-20 parts of an ethylene monomer, 5-10 parts of a reactive diluent, 0.1-5 parts of a chain transfer agent, 0.5-2.5 parts of an initiator, 0-10 parts of a solvent and 1-3 parts of a neutralizer. According to the invention, accurate regulation and control of key properties of the resin are realized by adjusting the monomer ratio and the type and dosage of the chain transfer agent: the number-average molecular weight covers 25,000-80,000, the glass-transition temperature is adjustable in the range of 25-80 DEG C, and different scene requirements of high hardness, high chemical resistance and the like are met; meanwhile, the hydrophobic core layer guarantees the mechanical strength, the semi-hydrophilic and semi-oleophylic shell layer improves the water dispersion stability, and the problem that high Tg / large molecular weight and dispersity of traditional water-based resin are difficult to consider at the same time is solved.

1. Name of the product in this design: Integrated High Performance Liquid Chromatograph. 2. Application of this design: It is mainly used for instruments and equipment for analyzing organic compounds with high boiling points, low volatility, unstable temperature, and large molecular weight. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention relates to the technical field of organic synthesis, and discloses a double-center non-metallocene catalyst and a preparation method thereof, and a butene-1-based elastomer and a preparation method thereof. Wherein the double-center non-metallocene catalyst comprises a structure as shown in a formula I; in the formula I, R1-R5 are independently selected from H or C1-C8 alkyl groups, R6 is selected from C1-C5 alkyl groups, aryl groups or aryl groups containing C1-C5 alkyl substituent groups, and R7 is selected from methyl and / or ethyl. When the double-center non-metallocene catalyst provided by the invention is used for catalyzing a polymerization reaction of butene-1 and long-chain alpha-olefin, the insertion rate of an alpha-olefin monomer with large steric hindrance can be increased, and the growth of a polymerization chain is facilitated; and the butene-1-based elastomer material with large molecular weight, narrow molecular weight distribution, low glass transition temperature and high random copolymerization unit content can be prepared.

The invention relates to the technical field of biological medicine, in particular to a TREM-1 targeted fluorescent molecular probe as well as a preparation method and application thereof, and discloses a fluorescent molecular probe named as' TREM-1 targeted fluorescent molecular probe as well as a preparation method and application thereof '. The invention aims to solve the problems of large molecular weight, poor pharmacokinetic performance, limited tissue penetration, high imaging background and poor imaging timeliness of the existing antibody fluorescent probe. The probe comprises a TREM-1 targeting module (TDM), a near-infrared fluorescence reporter group, a dark quenching group and a conformational sensitive connecting arm (CSL). The fluorescence of the probe is quenched before the probe is combined with TREM-1, and after the probe is combined with a target spot, TDM specific binding induces CSL conformation rearrangement, so that a reporter group and a quenching group are spatially separated, fluorescence resonance energy transfer (FRET) is relieved, and fluorescence activation is realized. According to the invention, rapid, high-contrast and low-background fluorescence imaging of atherosclerotic plaques is realized, and the probe is small in molecular size, strong in tissue penetrability and rapid in-vivo clearing.

The invention belongs to the technical field of biological detection, and particularly relates to a detection method and a detection kit for oxidized low-density lipoprotein. The invention provides a novel application of quaternary ammonium salt chitosan in a kit, and particularly relates to application of quaternary ammonium salt chitosan with different molecular weights in an immunoturbidimetry detection process of oxidized low-density lipoprotein. When high-molecular-weight quaternized chitosan with the molecular weight of 100-200 kDa and low-molecular-weight quaternized chitosan with the molecular weight of 30-50 kDa are used as components of the oxidized low-density lipoprotein detection kit, the quaternized chitosan with different molecular weights can react with citrate radicals to form gel particles with different mechanical strengths; the content of the oxidized low-density lipoprotein can be detected through immunoturbidimetry, and the sensitivity and the linear range of the oxidized low-density lipoprotein detection kit are greatly improved and widened by using the quaternized chitosan with different molecular weights.

The application provides a layer-by-layer self-assembly nanofiltration membrane, a preparation method and application thereof. The layer-by-layer self-assembly nanofiltration membrane comprises a base film and a self-assembly coating layer formed on the base film, wherein the self-assembly coating layer comprises at least a starting alternating coating layer and a separation alternating coating layer; the molecular weight cut-off of the base film is not less than 500 kDa; the starting alternating coating layer is alternately assembled by a high-molecular-weight cationic polyelectrolyte layer and an anionic polyelectrolyte layer; the separation alternating coating layer is alternately assembled by a low-molecular-weight cationic polyelectrolyte layer and an anionic polyelectrolyte layer; the starting alternating coating layer is formed on the base film, and the high-molecular-weight cationic polyelectrolyte layer in the starting alternating coating layer adopts a cationic polyelectrolyte with a number-average molecular weight of not less than 500 kDa; and the low-molecular-weight cationic polyelectrolyte layer in the separation alternating coating layer adopts a cationic polyelectrolyte with a number-average molecular weight of not more than 100 kDa. The layer-by-layer self-assembly nanofiltration membrane has more excellent permeability and micro-pollutant rejection rate.

The invention relates to a specifically degraded KIM1 liposome, preparation thereof and application of the specifically degraded KIM1 liposome in preparation of medicines for treating kidney diseases, and belongs to the technical field of biological medicines. According to the invention, a KIM1 targeting polypeptide and an E3 ubiquitin ligase ligand (such as lenalidomide) are respectively modified on the surface of a liposome. The lipidosome capable of specifically degrading the KIM1 can be rapidly enriched in a damaged kidney after renal injury occurs, and the KIM1 protein level in the damaged kidney is degraded by 75%. Meanwhile, the druggability problems of poor solubility, low bioavailability and the like caused by large molecular weight of the compound in the PROTAC drug development process are avoided, and the technical problems that drugs are difficult to prepare and kidney injury drugs are lacked in the prior art are solved.

The invention relates to the technical field of edible mushroom processing, in particular to low-molecular-weight hericium erinaceus beta-glucan with antioxidant activity as well as a preparation method and application of the low-molecular-weight hericium erinaceus beta-glucan. According to the method, the macromolecular hericium erinaceus beta-glucan is used as a raw material, the low-molecular-weight beta-glucan is prepared through microwave digestion and compound enzyme step-by-step degradation, the weight-average molecular weight of the prepared low-molecular-weight beta-glucan is 7.611 kDa, and compared with a high-molecular-weight beta-glucan sample, the low-molecular-weight beta-glucan is better in water solubility and lower in solution viscosity; the compound has the capability of efficiently scavenging free radicals, has good antioxidant activity, and has a wide application prospect.

The application discloses a kind of space-time controllable PROTAC based on photo-initiated in-situ generation and application thereof.The PROTAC is divided into two components: biorthogonal reaction group and the component of target protein ligand connection, biorthogonal reaction group and the component of E3 ubiquitin ligase ligand connection, can be synthesized in situ by light control PROTAC, for targeted protein degradation.It overcomes the difficulty of PROTAC molecule from scratch synthesis, synthesis step is complicated and the like, avoids the adverse pharmacological performance and toxic side effects caused by large molecular weight in traditional PROTAC design.At the same time, only after lightening, PROTAC molecule can be generated, the target protein is degraded, tumor cells are killed, off-target toxicity is avoided, and tumor treatment effect is realized.The method for generating protein hydrolysis targeting chimera by light triggering click provided by the application can controllably and efficiently synthesize PROTAC molecules with anticancer effect, and has the prospect of precise regulation of intracellular protein expression and treatment of tumor.

The invention discloses a positive photoresist composition suitable for an electroplating process as well as a preparation method and application of the positive photoresist composition, and belongs to the technical field of photoresist. The positive photoresist composition suitable for the electroplating process comprises the following components in parts by mass: 20-25 parts of high-molecular-weight high-sensitivity phenolic resin; 2 to 7 parts of plasticizing resin; 4-6 parts of a photosensitizer; 0.5-1 part of an additive; 61 to 73.5 parts of a solvent; wherein the molecular weight of the high-molecular-weight and high-sensitivity phenolic resin is 10000-20000, and the high-molecular-weight and high-sensitivity phenolic resin is low-ortho-position and high-branched phenolic resin in which the ratio of m-cresol to p-cresol is 8 / 2; the plasticizing resin is poly (p-hydroxystyrene) resin. The positive photoresist composition is suitable for an electroplating process, is used for solving the problem that a common photoresist is easy to break down in the electroplating process, and keeps good sensitivity characteristics at the same time.

The invention discloses polyurethane acrylate, an adhesive system of the polyurethane acrylate as well as a preparation method and application of the adhesive system. According to the invention, a polyurethane structure is introduced from the skeleton of bisphenol A, and polyurethane acrylate is designed and synthesized. The adhesive system comprises the following components in percentage by weight: 55-70wt% of a polymerizable diluent monomer, 10-20wt% of an initiator, 0.5-5wt% of a crosslinking agent, 0.5-5wt% of a crosslinking agent and 0.5-5wt% of an initiator, the content of the photoinitiator is 1-5 wt%; the content of the polyurethane acrylate is 25 to 40 weight percent; and the co-initiator is 1-2 wt%. By utilizing the toughness of a polyurethane molecular structure and the volume occupation effect of a bisphenol annular rigid structure, the concentration of polymerizable double bonds is reduced, the molecular thermal vibration amplitude before polymerization is reduced, and the intermolecular distance is shortened, so that the toughness of the resin is ensured, the polymerization shrinkage rate is reduced, and the adhesive property of the adhesive is improved. The adhesive prepared by the invention has the characteristics of moderate viscosity, small polymerization shrinkage, large molecular weight, good mechanical properties, and especially excellent toughness.

The application discloses an adsorbent for removing protein-bound toxins and middle-molecular toxins and a preparation method thereof, and belongs to the technical field of polymer materials, and comprises the following steps: polystyrene-based white ball preparation, amine immobilization and film coating.The application has the beneficial effects that: a kind of adsorbent capable of adsorbing protein-bound toxins and middle-molecular toxins is prepared by adding a pore-forming agent under the participation of styrene monomers, polyvinyl benzene series, glycidyl methacrylate and 4,4'-bis(methacrylamido)-azobenzene, the adsorption effect is good, and the adsorbent has high selectivity; the process method is simple, green and environment-friendly; glycidyl methacrylate and modified cyclodextrin are added, the hydrophilicity of the adsorbent is enhanced, the blood compatibility of the adsorbent is improved, and the adsorption effect of the adsorbent is enhanced through amine group immobilization.

Disclosed are an injection-type human collagen soft tissue filler material and a preparation method therefor. The present invention pertains to the technical field of biomedical materials. The injection-type human collagen soft tissue filler material comprises a pretreated recombinant human collagen and an osmotic pressure regulator and is in an isotonic state. The pretreatment of the recombinant human collagen is isoelectric precipitation and centrifugal separation. The preparation method therefor comprises sterilizing, by means of filtration, and pretreating raw materials, and homogenizing the pretreated recombinant human collagen and the osmotic pressure regulator. The injection-type human collagen soft tissue filler material of the present invention mainly comprises human collagen, has a large molecular weight and a triple-helix structure, has a uniform particle size distribution, and can achieve a filling and supporting effect. The preparation process for the material is simple and convenient, involves no special treatment process, does not introduce toxic and hazardous substances that tend to remain, and stabilizes the performance of products.

The application belongs to the field of fluorescent probes, and particularly relates to a tumor-targeting photothermal reagent based on fluoro-rhodamine, and a preparation method and application thereof. To solve the problems that most of existing photothermal reagents have difficulty in post-modification due to complex synthesis, poor biological compatibility due to large molecular weight, and lack of tumor targeting of these molecules, and generally need to be made into nanoparticles to enrich to the tumor site by EPR effect, the application develops a tumor-targeting photothermal reagent Tag-880-NHS based on fluoro-rhodamine, which has the characteristics of simple synthesis, good water solubility, good light stability, high photothermal conversion efficiency, and an absorption wavelength located in the NIR region, and can be used for labeling a specific targeting epidermal growth factor monoclonal antibody-panitumumab, and can be used in the fields of tumor imaging and treatment, and has important application value.

The invention discloses extra-thick organic glass and a preparation method thereof. A direct casting method is adopted, pressure is provided through an automatic hydraulic mold, and different active initiators are compounded for use, so that the uniformity of the polymerization rate is improved. The preparation method comprises the following steps: carrying out prepolymerization on 99 to 99.9 weight percent of methyl methacrylate and 0.01 to 0.5 weight percent of initiator A in a reaction kettle at the temperature of 75 + / -5 DEG C; reducing the temperature, and adding 0.01 to 0.5 weight percent of an initiator B and 0.01 to 0.5 weight percent of an initiator C to prepare a prepolymer; after vacuum defoaming, pouring into an automatic hydraulic mold, and carrying out water bath low-temperature polymerization; and carrying out high-temperature post-treatment, cooling and demolding to obtain the ultra-thick organic glass. Compared with the prior art, the prepared ultra-thick organic glass is uniform in polymerization rate, large in molecular weight and good in optical performance and mechanical strength, the thickness can reach 300 mm, and requirements of special industries are met.

The invention relates to dendrobium polysaccharide as well as a preparation method and application thereof. The method comprises the following steps: preparing dendrobium into dendrobium powder; performing carbon dioxide supercritical extraction on the dendrobium nobile powder by using ethanol as an entrainer to obtain dendrobium nobile powder; performing water extraction on the dendrobium nobile extract powder to obtain a dendrobium nobile water extract; and drying the dendrobe water extract to obtain the dendrobe polysaccharide. The dendrobium polysaccharide obtained by the method is high in purity and large in molecular weight, and has the effects of dispelling the effects of alcohol and protecting liver and intestinal mucosa; the method is simple and convenient to operate, toxic reagents are not introduced in the whole process, the preparation process is green, natural and safe, and high yield, high purity and food safety are considered.

The application provides a macromolecular titanate coupling agent and a preparation method thereof, which is obtained by using an ester exchange polycondensation method on small molecule titanate coupling agent and dihydric alcohol monomers, and is polymerized in a gradient heating mode under a nitrogen atmosphere, and synthesis steps are as follows: under a nitrogen atmosphere, the small molecule titanate coupling agent and the dihydric alcohol monomers are added into a three-necked flask, the three-necked flask is heated through an oil bath, first, the heating temperature is increased from room temperature to 60-120 DEG C, and is kept for 3-5 hours, then the heating is continuously increased to 140-160 DEG C, and is reacted for 3-5 hours, then the heating is continuously increased to 175-180 DEG C, and is reacted for 1-3 hours. The macromolecular titanate coupling agent is obtained by using the ester exchange polycondensation method on the small molecule titanate coupling agent and the dihydric alcohol monomers, the coupling agent can avoid the problem that the alkoxyl in the structure of the titanate coupling agent is easy to contact with water vapor in the air and hydrolyze due to the characteristics of large molecular weight and linear / branched structure, and the macromolecular coupling agent structure still contains characteristic functional groups, so that the functionality of the coupling agent can be reserved.

To provide a method for producing a polyoxypropylene-based polymer, by which the polyoxypropylene-based polymer having a large molecular weight and exhibiting a low viscosity due to a narrow molecular weight distribution can be produced without using a large amount of water.SOLUTION: The polyoxypropylene-based polymer having a design molecular weight value X (g / mol) of 12,000g / mol or more and 30,000g / mol or less is obtained by setting the internal temperature of a reaction vessel charged with an initiator, a double metal cyanide complex catalyst, and propylene oxide to 90 °C or higher and 110 °C or lower. Activating the double metal cyanide complex catalyst, and after adjusting the internal temperature of the reaction vessel to a temperature of at least 90 °C and at most 110 °C, carrying out polymerization by additionally adding propylene oxide to the reaction vessel over Y minutes under a condition of at least 120 °C and at most 160 °C, wherein the value of X / Y is at least 35 and at most 67.SELECTED DRAWING: None

The invention discloses an efficient 5 '-UTR element based on a human CCND1 gene and application of the efficient 5'-UTR element in enhancement of exogenous gene expression, and belongs to the technical field of gene engineering. The nucleotide sequence of the screened and optimized 5 '-UTR element is as shown in SEQ ID NO: 1-3, and the 5'-UTR element is proved to be capable of remarkably improving the yield of target protein; no matter fluorescent protein or protein with large molecular weight, the 5 '-UTR can improve the expression quantity of the protein, and has strong universality; the 5 '-UTR provided by the invention does not introduce a long fragment or additional amino acid sequence, and the 5'-UTR is derived from human, so that the immunogenicity and unexpected secondary structure risk can be reduced. Meanwhile, the length is 58-158 nt, and the small fragment is beneficial to packaging of the virus vector and can also be matched with the virus vector. Therefore, the 5 '-UTR provided by the invention has a relatively good application prospect.

This invention discloses a nanozyme for the detection of phenolic compounds and a quantitative detection method for phenolic compounds. The nanozyme comprises an organometallic framework material and copper loaded on the surface of the organometallic framework material, possessing a core-shell structure. The active metal catalytic center is loaded on the material surface, which helps to improve the utilization efficiency and catalytic activity of the active center, thereby enhancing the detection capability of large molecular weight phenolic compounds. Based on the standard addition method, the nanozyme of this invention can be used for the quantitative analysis of BPA in environmental water and thermal paper, exhibiting high accuracy and sensitivity, and strong anti-interference ability. The quantitative detection method of this invention can be used in conjunction with an image acquisition device for quantitative calculation of phenolic compounds, further simplifying the detection of phenolic compounds.

The application discloses a method for screening shikonin acting target points based on LIP-MS technology, and relates to the technical field of medicines, and has the technical features that the application adopts a widely specific protease to perform limited enzymolysis on a protein sample, utilizes the characteristic that drug and target protein combination can improve the conformation stability of the combination region peptide segment, further uses trypsin to perform secondary enzymolysis, collects a peptide fingerprint by using quantitative proteomics technology, analyzes and compares the changes of the peptide fingerprints of a control group and a drug treatment group, screens out the specificity of the 'conformational retention peptide segment' after drug treatment, identifies the target protein information corresponding to the peptide segment by database matching, compared with the traditional DARTS method, the non-labeled drug target analysis based on the peptide fingerprint changes the detection object from the protein into the peptide segment, and is expected to characterize the low-affinity multi-target drug and protein interaction, low-abundance protein, large molecular weight protein local conformation change information, so as to improve the accuracy and sensitivity of target analysis.

The application provides a preparation method of alumina pellets with large specific surface area and large pore volume. 20 PO 10 EO 20 As a template inducer with large molecular weight and stable structure, it can spontaneously and orderly aggregate to form micelles in aqueous solution, and the aluminum source can self-assemble along the outer surface of the micelles after hydrolysis in the solution, so that the template induces the crystallization to control the morphology of crystal growth and regulate the size of the crystal, and promotes the increase of the order degree of the crystal and the uniform distribution of the crystal grains, and after the crystallization treatment, a stable and ordered mesoporous structure is formed. The powder obtained by the method has the characteristics of expanding the pore structure and the specific surface area, improving the loading performance and increasing the dispersion of the active component.