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Fractional extraction method for honey pomelo peel polysaccharides, product thereof and application of product

A grading method, the technology of pomelo peel polysaccharide, applied in the application, food science, tobacco and other directions, to achieve good results

Active Publication Date: 2014-01-15
CHINA TOBACCO GUANGDONG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the application of HSDE combined with compound enzyme secondary wall breaking technology in the extraction of pomelo peel polysaccharides

Method used

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  • Fractional extraction method for honey pomelo peel polysaccharides, product thereof and application of product
  • Fractional extraction method for honey pomelo peel polysaccharides, product thereof and application of product
  • Fractional extraction method for honey pomelo peel polysaccharides, product thereof and application of product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] S1. Take pomelo peel, add 1.5% cellulase and 1.5% pectinase according to the weight of pomelo peel, add water at 50°C, the weight ratio of water and pomelo peel is 25:1, high shear Dispersing and emulsifying machine 10000r / min under the treatment of 10min, enzymatic hydrolysis for 1.5 hours, to obtain enzymatic hydrolysis liquid, take a part of the enzymatic hydrolysis liquid, leaching, to obtain a polysaccharide content of 9.49%

[0040] S2. Take the enzymolysis solution obtained from S1, add ethanol so that the concentration of ethanol in the solution is 45~55%, ethanol analysis at a temperature of 25°C-4°C, centrifuge, and centrifuge to obtain a precipitate 1 and a supernatant 1, and the obtained precipitate Object one is that the yield of a crude polysaccharide is 13.12%, the purity of the polysaccharide in the a crude polysaccharide is 42.2%, and the yield of a polysaccharide after purification is 5.6%,

[0041]S3. Take the supernatant liquid 1 obtained in step S2...

Embodiment 2

[0044] Example 2 Effects of solid-liquid ratio, enzyme amount, enzymatic hydrolysis time and enzymatic hydrolysis temperature on extraction.

[0045] 1. Materials

[0046] The honey pomelo was collected from the pomelo garden in Meizhou, and the honey pomelo with the same variety, size, uniform fruit color, basically the same maturity, no mechanical damage, no disease and pest infestation was selected as the test material. After the peel is cut into thin slices, it is dried in a constant temperature drying oven, and it is ground into powder by a pulverizer for later use. Reagents were analytically pure glucose, absolute ethanol, propylene glycol, 5% phenol, concentrated sulfuric acid, deionized water, chloroform, n-butanol, activated carbon, etc. were purchased from Guangzhou Yima Biotechnology Co., Ltd. Pectinase: Tianjin Enzyme Preparation Factory; Cellulase: Shanghai Boao Biotechnology Co., Ltd.

[0047] 2. Instrument

[0048] DU-730 UV-Vis Spectrophotometer (S...

Embodiment 3

[0097] Example 3 Application of a polysaccharide, b polysaccharide and c polysaccharide in keeping tobacco moist

[0098] 1. Determination of self-moisture retention of pomelo peel polysaccharides of different grades

[0099] Using 30% ethanol and deionized water as solvents respectively, at room temperature, take 2 g each of crude polysaccharide a, crude polysaccharide b, and crude polysaccharide c with a concentration of 1.5%, and 2 g each of 0.2% propylene glycol and 1.5% propylene glycol as The control group was placed in a watch glass, a total of 8 groups. At room temperature, every 1h, the samples were taken out and weighed, and the water loss rate was calculated according to the formula: water loss rate (%)=(M 1 -M 2 ) / M 3 ×100.

[0100] (where: M 1 is the total weight of the sample before placing; M 2 is the total weight of the sample after being placed for different times; M 3 is the net weight of the sample)

[0101] The specific effect is shown in Fig. Fig...

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Abstract

The invention relates to a fractional extraction method for honey pomelo peel polysaccharides, a product thereof and application of the product, and provides a method for preparing a tobacco humectant from honey pomelo peels. According to the invention, the honey pomelo peel polysaccharides are extracted by taking honey pomelo peels as raw material and combining high-speed shear emulsification method with complex enzyme two-stage cell wall-breaking method, the honey pomelo peel polysaccharides are fractionated using different solvents by means of organic solvent temperature-variable penetration and precipitation, and the difference in molecular weight distribution of fractionated polysaccharides is investigated by means of gel chromatography. The molecular weight determination of different fractionated polysaccharides shows that there is a significant difference in molecular weight of polysaccharides which are fractionated using different solvents, wherein the weight-average molecular weight of the peak (1) of crude polysaccharide a is 24100 Da, the weight-average molecular weight of the peak (1) of crude polysaccharide b is 11700 Da, and the weight-average molecular weight of the peak (1) of crude polysaccharide c is 2130 Da. As is indicated that the lower the ethanol concentration, the larger molecular weight of the precipitated polysaccharides. The optimum water-retaining concentration of pomelo peel polysaccharides is 1.5 g / 100 ml. When different solvents are selected, the water-retaining effects of pomelo peel polysaccharides are different, and the water-retaining effect of water-soluble pomelo peel polysaccharides is significantly higher than that of alcohol-soluble pomelo peel polysaccharides. In general, the water-retaining effects of both water-soluble pomelo peel polysaccharides and alcohol-soluble pomelo peel polysaccharides are better than those of propylene glycol and other humectants used in the prior art.

Description

technical field [0001] The invention relates to the field of polysaccharide extraction, more specifically, to a method for extracting and grading pomelo pomelo peel polysaccharides and the application of the product. Background technique [0002] Pomelo belongs to Rutaceae citrus and is one of the most popular fruits. Guangdong Meixian pomelo is the leading product of the "three high" agricultural strategy in Guangdong Province. At present, Meizhou golden pomelo has been cultivated for nearly a hundred years. The total area of ​​the city is 400,000 mu, and the output is 300,000 tons. Above, the output of pomelo in the province is as high as 400,000 tons, and it is the largest production base of pomelo in the country. In 1995, it was named the "Hometown of Golden Pomelo" by the Organizing Committee of the first batch of 100 Chinese Specialty Hometowns. At present, the city has a total area of ​​300,000 mu and an output of more than 300,000 tons. It is the largest production ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A24B15/40
Inventor 冯志斌陶红郭文卓浩廉
Owner CHINA TOBACCO GUANGDONG IND
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