723 results about "Cellulase" patented technology

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention provides Aspergillus tubingensis HNZY02, a method for producing enzymes by the Aspergillus tubingensis HNZY02 and application of the Aspergillus tubingensis HNZY02, and belongs to the technical field of microorganisms, the Aspergillus tubingensis HNZY02 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.42148. The aspergillus tubingensis HNZY02 shows excellent cellulase and xylanase synthesis capability, can synergistically and efficiently degrade main structural components such as cellulose, hemicellulose and lignin in tobacco stems, and improves the safety and application value of cigarettes while breaking the compact structure and increasing the reducing sugar content.

The invention relates to the technical field of soil amendments, in particular to a soil amendment containing earthworm enzymatic hydrolysate and a preparation method of the soil amendment. The soil conditioner containing the earthworm enzymatic hydrolysate comprises the following components: earthworm enzymatic hydrolysate microcapsules and adjuvant particles in a mass ratio of (2-4): 1, the earthworm enzymatic hydrolysate is prepared by performing enzymolysis on fresh earthworm homogenate by compound enzyme, and the compound enzyme comprises cellulase and protease; the wall material of the earthworm enzymatic hydrolysate microcapsule comprises at least one of chitosan, calcium alginate, gelatin and Arabic gum; the auxiliary agent comprises humic acid and inorganic natural minerals. According to the soil conditioner containing the earthworm enzymatic hydrolysate, the earthworm enzymatic hydrolysate is wrapped by the microcapsules, the retention rate of active ingredients in the earthworm enzymatic hydrolysate can be remarkably increased, and the soil condition can be further improved by matching with humic acid and inorganic natural minerals for use.

The invention relates to a medicine for skin regeneration and repair and a preparation method thereof, and belongs to the technical field of biological medicine, the medicine comprises cyperus esculentus extract peptide, brooklet anemone root tremella extract, rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract, pomegranate peel polyphenol and the like. The cyperus esculentus extracted peptide is a product which is more than 1kDa and is obtained by performing enzymolysis on cyperus esculentus through cellulase, alpha-chymotrypsin and ficin protease in sequence and then performing purification through a G-50 sephadex column; the tremellodon gelatinosum tremella extract is a product which is less than 3kDa and is obtained by performing common enzymolysis on tremellodon gelatinosum tremella sporocarp through cellulase and bromelain; the rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract is a product obtained by performing gradient extraction on rhodomyrtus tomentosa root, suberect spatholobus stem and radix cyathulae through an ethanol water solution to obtain an extract, and performing polyamide resin column adsorption and LH-60 sephadex column purification in sequence. A good repairing system is constructed by multiple components, and wound repairing and healing are accelerated.

Disclosed is a method for reducing the usage amount of edible fat and oil in a bakery product, the method comprising mixing at least one maltose alpha-amylase and edible fat and oil into dough, and baking same to prepare a bakery product. The usage amount of the edible fat and oil in the dough can be reduced by at least 10 wt % compared with not using the enzyme treatment; and the enzyme may also include cellulase and / or phospholipase. The method does not reduce or substantially reduce the quality of the bakery product, and allows same to have a shelf life of at least 4 days. Also involved is baked fat and oil prepared from the above-mentioned enzyme and edible fat and oil.

The invention relates to the technical field of food, in particular to geobacillus stearothermophilus cKM-Geo, application of the geobacillus stearothermophilus cKM-Geo, a shrimp shell oligosaccharide peptide compound and a preparation method of the shrimp shell oligosaccharide peptide compound. The invention provides geobacillus stearothermophilus cKM-Geo, the strain can grow in an oxygen-free environment, ventilation is not needed in the process, and proteolytic enzyme, cellulase, lipase and chitin deacetylase can be highly secreted and expressed; the invention further provides a preparation method for preparing the shrimp shell oligosaccharide peptide by utilizing geobacillus stearothermophilus cKM-Geo fermentation coupling steam explosion treatment, chitin and protein in the shrimp shell are converted into water-soluble oligopeptide and oligosaccharides, the water-soluble oligopeptide and oligosaccharides are separated from calcium carbonate in the shrimp shell, acid and alkali do not need to be used, in the process, waste water is little, pollution is little, and the method is suitable for industrial production. The resource utilization rate is high.

The invention relates to the technical field of wine brewing, and discloses a bamboo leaf powder added distilled liquor and a preparation method thereof, and the distilled liquor is prepared from 75-85 parts of grain powder and 15-25 parts of enzyme-deactivated bamboo leaf powder. The preparation method comprises the following steps: mixing the raw materials to obtain a dry mixture; adding a compound enzyme preparation containing cellulase and pectinase, preserving heat at 50-55 DEG C, performing enzymolysis and moistening, destroying a fiber structure and converting fermentable sugar; cooking the moistened material, and adding a saccharifying enzyme for saccharifying; primary fermentation is performed at 28-32 DEG C, and then secondary fermentation is performed at 22-25 DEG C; during distillation, foreshot with the expected wine yield of 1.5%-2.0% is intercepted and abandoned to remove methanol, fractions with the volume of 50% or above are collected to obtain raw wine, and the wine is obtained through ageing. The raw material utilization rate and the wine yield are improved through enzymolysis, the methanol content is controlled through temperature-variable fermentation and precise cut distillation, the raw and green taste of bamboo leaves is eliminated, and the finished wine has the unique composite style of bamboo fragrance and grain fragrance.

The invention relates to a multi-component ginseng extract containing ginseng polypeptide and a preparation method of the multi-component ginseng extract. The preparation method comprises the following steps: S1, preparing ginseng powder from a ginseng raw material; mixing the ginseng powder with an acidic buffer solution, and carrying out presoaking treatment and homogenate dissolution promoting treatment to obtain ginseng primary pulp; s2, mixing the wall-breaking enzyme system with the ginseng primary pulp, and performing acid wall-breaking treatment to obtain wall-broken primary pulp; mixing an invertase system with the wall-broken primary pulp, and carrying out enzymolysis synchronous glucoside conversion treatment to obtain a crude extract; s3, filtering and separating the crude extracting solution to obtain an extract containing the ginseng polypeptide; the pH values of the ginseng primary pulp, the wall-broken primary pulp and the crude extract are in a range of 4.0-6.0; the wall breaking enzyme system comprises pectinase and cellulase; the invertase system comprises beta-glucosidase and acid protease. The technical problems that in the prior art, a ginseng extract is complex in extraction process, multiple active ingredients are difficult to efficiently extract at the same time, the product quality is low, and the biological activity is poor are solved.

The invention belongs to the technical field of microorganisms, and particularly discloses bacillus amyloliquefaciens QGFCH3-3 as well as a microbial agent and application of the bacillus amyloliquefaciens QGFCH3-3. The preservation number of the bacillus amyloliquefaciens QGFCH3-3 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.34814 The strain has the functions of nitrogen fixation, amylase production, ligninase production, protease production and cellulase production, can effectively promote plant growth, has broad-spectrum antibacterial activity, can significantly inhibit various pathogenic bacteria, and significantly improves the disease control effect of crops.

The invention relates to the technical field of deep processing of vegetable fat, in particular to a preparation method and application of squalene-containing macadamia nut oil. Through the synergistic effect of directional enzymolysis conversion and low-temperature step-by-step extraction and the combined effect of the three enzymes (cellulase destroys cell walls, lipase releases grease and beta-glucosidase removes inhibitors, and the three enzymes form a wall breaking-oil releasing-synergistic synergistic reaction) by utilizing a compound enzyme which is fixedly matched and proportioned. The compound enzyme can be used for synchronously treating cellulose, lipid and glycoside substances, so that the release efficiency of squalene is improved; and step-by-step extraction is utilized to reduce extraction impurities, so that the content of squalene in the macadamia nut oil can be increased, and the extraction and enrichment of squalene in the macadamia nut meat can be realized.

The invention discloses a liver protection composition containing glutathione-rich yeast. The liver protection composition is prepared from the glutathione-rich yeast, turmeric, silybum marianum, enzymolysis polypeptide with homology of medicine and food and anti-inflammatory and anti-oxidation auxiliary components. Wherein the medicine and food homologous enzymolysis polypeptide is prepared from radix puerariae through neutral protease-cellulase double enzymolysis, the polypeptide sequence is identified as Leuu-Val-Glu-Pro-Ala, and the medicine and food homologous enzymolysis polypeptide has the capacity of remarkably inhibiting LPS-induced RAW264.7 cell NO release and removing DPPH free radicals. According to the composition, through multi-component synergism, in an anti-alcohol experiment, the blood alcohol concentration decline rate of a mouse can be remarkably increased, the MDA content of liver tissue is remarkably reduced, and no acute toxic reaction exists. The composition has the functions of protecting the liver and dispelling the effects of alcohol, is safe and effective, and is suitable for the field of health-care products or functional foods.

The invention belongs to the technical field of microorganisms and fermentation, and discloses a method for synergistically fermenting ginseng powder through bacteria and enzymes, acetobacter aceti, ginseng spice and application, and the method comprises the following steps: (1) preparing a ginseng powder liquid culture medium; (2) inoculating acetobacter aceti and cellulase, and performing liquid fermentation for 24-36 hours; (3) inoculating amylase, and continuously fermenting for 96-120 hours; and (4) after fermentation is finished, supplementing ethanol until the final volume concentration of ethanol is 45-55%, stirring at normal temperature for 24 hours, standing, settling and filtering to obtain supernate, namely the ginseng spice. According to the preparation method, the earthy smell of the traditional ginseng spice can be remarkably reduced by the obtained ginseng spice through technologies such as bacterium-enzyme synergy and specific microbial fermentation metabolism control, meanwhile, the ginseng spice has typical aroma characteristics and is high in suckability, and meanwhile, the product quality of ginseng spice series cigarettes is effectively improved.

This invention discloses an integrated micro-aqueous degumming and peeling method for fresh coffee cherries, belonging to the field of fresh coffee cherry processing technology. The method includes: micro-aqueous degumming of fresh cherries using a pretreatment solution containing sodium citrate and sodium chloride; biological degumming using a specialized composite degumming enzyme composed of pectinase, cellulase, and calcium chloride; adding chitosan to the wastewater after rinsing for composite coagulation, followed by solid-liquid separation to obtain recycled water; and finally drying to obtain coffee beans in their shells. This invention utilizes sodium citrate to chelate calcium bridges to degrade the pectin cross-linking structure and sodium chloride to compress the double electric layer and reduce slurry viscosity, achieving efficient mass transfer and rapid degumming under micro-aqueous conditions. Furthermore, it utilizes residual salts to achieve pH anchoring and ionic strength regulation, promoting the formation of a dense aggregated phase between pectin degradation products and chitosan, solving the industry problems of difficult micro-aqueous system treatment and unstable recycled water reuse. This invention reduces water consumption by more than 90% compared to traditional washing methods, achieves thorough degumming, preserves the complete flavor of coffee beans, has low equipment costs, and enables sustainable closed-loop production, making it suitable for industrial application in small-scale coffee processing plants.

The application provides a fattening late-stage pig feed and a preparation method thereof, and relates to the technical field of pork breeding. The fattening late-stage pig feed is characterized by adding 10-15% of a nutritional supplement to per kilogram of fattening feed, and the nutritional supplement is composed of pine branch fermentation material, lotus root fermentation material, alfalfa powder, roxburgh rose extract and caffeine. The pine branch fermentation material is obtained by subjecting pine branches to cellulase enzymolysis treatment and then to fermentation by means of bifidobacterium and spencerolyn cryptococcus. The lotus root fermentation material is obtained by subjecting cooked lotus roots to fermentation by means of lactobacillus plantarum. The application overcomes the shortcomings of the prior art, effectively guarantees the weight gain efficiency of porkers in the late fattening stage, further improves pork quality, and comprehensively improves the economic benefits of pork breeding.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The invention belongs to the field of food engineering and traditional Chinese medicine functional foods, and discloses a preparation method of a tremella and ginseng composite freeze-dried product, which comprises the following steps: S1, preparing a decoction, weighing poria cocos, Chinese yam and other medicinal materials and ginseng slices according to a ratio, soaking, taking out the ginseng slices for later use, decocting the remaining medicinal materials, and filtering to obtain the decoction; s2, preparing a radix ophiopogonis zymolyte, namely crushing radix ophiopogonis, dissolving, adding a compound enzyme (cellulase: pectinase = 2: 1) for enzymolysis, and performing enzyme deactivation and concentration to obtain the zymolyte; s3, the tremella is boiled to produce glue, the soaked tremella and the medicine soup are mixed and boiled, and the radix ophiopogonis zymolyte, the ginseng powder and the sweetening agent are added; s4, molding and filling into a mold: filling the white fungus thick soup into the mold, and embedding the soaked ginseng slices, Chinese wolfberry fruits and red date slices; and S5, freeze-drying and packaging, namely sealing after pre-freezing and sublimation drying. According to the preparation method, the rehydration time of the product is less than or equal to 67 seconds, the retention rate of ginsenoside is greater than or equal to 90% and the distribution of ingredients is uniform and visual through segmented ginseng treatment (slice embedding and powder supplementing), radix ophiopogonis enzymolysis and process parameter standardization, so that the preparation method is suitable for industrial production of health-care foods and ready-to-eat foods.

This invention discloses a low-temperature enzymatic hydrolysis compound beverage made from edible and medicinal plants, and its preparation method. Using jujube powder as a base, at least two kinds of dried powders from medicinal and edible plants are compounded. Enzymatic hydrolysis is performed using a compound enzyme containing cellulase, pectinase, and acidic protease at 45-50℃ and pH 4.0-5.0. The product is then subjected to enzyme inactivation and solid-liquid separation (filtration / centrifugation or a combination thereof). Amylase can be added for high-starch / polysaccharide raw materials. The product contains no added flavorings, colorings, sweeteners, or preservatives. After 12 months of storage at room temperature, the sedimentation rate is ≤0.5%, and the retention rate of active ingredients is 26%-31% higher than that of traditional high-temperature processes. The process is highly versatile and can be adapted to different separation methods, enabling the industrial production of clean-label, highly stable plant-based beverages.

The invention belongs to the technical field of plant cytology, and particularly relates to a method for improving the transformation efficiency of plant protoplast, which comprises the following steps: preparing enzymatic hydrolysate: each liter of enzymatic hydrolysate contains 20 mmol of KCl, 5-20 g of cellulase, 2-6 g of macerozyme, 10 mmol of CaCl2, 1 g of BSA and the balance of water; performing leaf enzymolysis; obtaining a protoplast; preparing a competent protoplast; resuspending the MMG solution; and transforming and incubating the protoplast resuspension by using the target plasmid. According to the method, the high-quality protoplast can be obtained, and when the protoplast is applied to downstream experiments, the conversion efficiency and stability of the experiments can be remarkably improved.

The invention provides a biological sewage treatment accelerant as well as a preparation method and application thereof, and belongs to the technical field of sewage treatment. According to the application, the fruit and vegetable fermentation liquor, the inorganic carbon source, the lipase, the protease, the amylase, the cellulase, the B vitamins, the cytochrome c, the coenzyme Q and the ethylene glycol are used as main components to prepare the accelerant, the accelerant is added into a biological sewage treatment reaction tank, and the biological sewage treatment effect is enhanced by increasing the microbial nitration reaction and denitrification reaction rate.

This invention relates to the field of fertilizer technology, specifically to an organic liquid fertilizer based on straw enzymatic hydrolysate and its preparation method. The method includes: pretreating corn straw with potassium hydroxide, followed by enzymatic hydrolysis with cellulase and xylanase to obtain the enzymatic supernatant; sequentially treating the supernatant with an ultrafiltration membrane with a molecular weight cutoff of 3000 Da and a nanofiltration membrane with a molecular weight cutoff of 500 Da to collect oligosaccharides with a molecular weight window of 500-3000 Da; partially stabilizing the reducing end of the oligosaccharides with sodium borohydride; pre-complexing with boric acid at pH 8.4-8.8; then sequentially adding relatively low molecular weight sodium polyglutamate, calcium chloride dihydrate aqueous solution, and ultra-high molecular weight sodium polyglutamate to construct a sequential complexation system; subsequently adding potassium nitrate and citric acid aqueous solution; and finally, after filtration at 5 μm and heat treatment at 70℃, obtaining the organic liquid fertilizer. This fertilizer has a high proportion of 500-3000 Da oligosaccharides and a low reducing sugar equivalent, inhibiting storage precipitation and browning, and improving the retention rate of extractable oligosaccharides in the soil.

The application discloses a method for preparing an oat-based beverage through biological enzymolysis and a product thereof, and comprises the following steps: continuously hydrolyzing starch, cellulose, protein and other substances in oat slurry by using high-temperature-resistant amylase, cellulase, saccharifying enzyme, papain and alkaline protease, sufficiently releasing reducing substances in the oat, simultaneously generating reducing peptides, improving the free radical scavenging capacity of the oat slurry, and improving the emulsification stability of the oat slurry due to the moderate degradation of macromolecular substances such as starch, cellulose and protein; glycerol is added in three stages at a controlled speed, so that the glycerol is fully mixed with flaxseed oil, and excessive glycerol is avoided from adhering to lipase to reduce the activity of the enzyme, thereby improving the reaction efficiency.

This application relates to a denim washing anti-staining agent and its application, belonging to the field of dyeing and printing auxiliaries technology. The anti-staining agent is composed of the following components in parts by weight: 5-15 parts of polymeric dispersant, 5-10 parts of anionic dispersant, 3-5 parts of isomeric tridecyl alcohol polyoxyalkylene ether, 3-5 parts of fatty amine polyoxyethylene ether, 0.5-2 parts of polyether defoamer, with the balance being water. This application endows the product with excellent immediate anti-staining ability and outstanding continuous processing stability, and is suitable for the cellulase washing process of denim.

The invention discloses a bacterium-enzyme compound feed additive and a preparation method thereof, and belongs to the field of compound functional feed additives, the bacterium-enzyme compound feed additive comprises the following raw materials in parts by weight: 10-30 parts of compound probiotics, 10-30 parts of a compound enzyme preparation, 20-30 parts of a protein-based microcapsule coating material, 5-10 parts of thyme essential oil and 3-8 parts of prebiotics. Wherein the compound probiotics comprise bifidobacterium, lactobacillus and bacillus subtilis according to the mass ratio of 1: (0.3 to 0.7): (0.3 to 0.7); the compound enzyme preparation comprises at least two of cellulase, beta-mannase, lactase, protease and lipase. By adding the bacterium-enzyme composite feed additive prepared by the invention, the proportion of beneficial bacteria in intestinal tracts and the absorption and utilization rate of feed nutrients can be improved.

The present application belongs to the technical field of pulping and papermaking, and provides a method for preparing a cotton-stalk biochemomechanical pulp. The present application provides a method for preparing a cotton-stalk biochemomechanical pulp, the method comprising the following steps: cutting cleaned and dried cotton stalks, and then sequentially performing pre-steaming and a screw extrusion fiberization treatment; immersing the defibrated cotton stalks into a buffer solution of cellulase to perform an enzymatic treatment for a set period of time, and then immersing same into a hot alkali solution; sequentially subjecting the cotton stalks, which have been subjected to a hot alkali impregnation treatment, to high-consistency pulp refining and a latency removal treatment, and then subjecting the resulting coarse pulp to a second-stage enzymatic treatment by using a compound enzyme of xylanase and pectinase; and subjecting the pulp, which has been subjected to the second-stage enzymatic treatment, to pulp screening and PFI beating, so as to obtain a cotton-stalk biochemomechanical pulp.

This disclosure provides a method for extracting bioactive substances from microalgae in high-salt-alkali environments, specifically including low-temperature plasma pretreatment; mixed enzymatic hydrolysis to break down cell walls, the mixed enzymes including cellulase and pectinase; ultrasonic extraction with a salt-tolerant extract; and purification by aqueous two-phase extraction followed by hydrophobic chromatography. The technical solution disclosed in this invention solves the problems of low extraction rate, high cost, and salt-alkali interference in the existing extraction of bioactive substances from high-salt-alkali microalgae, achieving (1) increasing the extraction rate of bioactive substances from high-salt-alkali microalgae to ≥90%; (2) shortening the purification process to ≤24 hours, with a target product purity of ≥95%; and (3) reducing the interference of the salt-alkali environment on the extraction process and reducing the amount of organic solvent used by more than 30%.

The invention provides a quinoa flavone extracting solution as well as a preparation method and application thereof, and belongs to the technical field of natural plant extraction. The preparation method of the quinoa flavone extracting solution provided by the invention comprises the following steps: mixing quinoa, compound enzyme and water to obtain a mixed solution; performing ultrasonic treatment on the mixed solution to obtain a quinoa flavone extracting solution; the compound enzyme comprises cellulase and pectinase. According to the method, the flavone in the chenopodium quinoa willd is extracted by adopting ultrasonic-compound enzyme synergistically, so that the method has the characteristics of high yield, short extraction time, low energy consumption, large raw material treatment capacity, low possibility of damaging active substances, environmental friendliness, reduction of environmental pollution and the like, the comprehensive utilization of the chenopodium quinoa willd can be enhanced, and the additional value of the chenopodium quinoa willd can be improved.

The invention relates to the technical field of biological waste treatment, in particular to a preparation method and application of a silkworm sand feed additive. The invention provides a technical scheme for preparing a silkworm and mulberry sand feed additive rich in acid protease, pectinase and cellulase by taking silkworm sand as a core matrix and performing directional pretreatment, adaptive strain fermentation and standardized post-treatment. According to the invention, silkworm sand-corncob powder is used as a composite substrate, and the silkworm sand and the corncob powder complement each other in function, so that the problems of unbalanced nutrition and poor air permeability of a single substrate are solved. Specific aspergillus niger is used for segmented solid-state fermentation, parameters are set in a differentiated mode, and full-amount utilization of the silkworm sand is achieved; the product quality and enzyme activity are improved by optimizing the process, and the resource value is improved from the low-added-value field to the high-added-value enzyme preparation industry.

The application belongs to the technical field of food processing, and particularly relates to a preparation method of a portable freeze-dried Chinese wolfberry polysaccharide solid beverage, which comprises the following steps: taking Qaidam Chinese wolfberry pomace as raw material, after pretreatment, using cellulase-assisted ultrasonic extraction and vacuum low-temperature concentration to obtain a Chinese wolfberry polysaccharide solution; then mixing the Chinese wolfberry polysaccharide solution with a probiotic auxiliary material mixture uniformly in a sterile manner, pre-freezing, freeze-drying and then dispensing to obtain a finished product. The application adopts an 'enzymatic cold extraction and vacuum low-temperature concentration' process, greatly improves the extraction rate and activity of Chinese wolfberry polysaccharide, shortens the freeze-drying time, and the prepared solid powder is portable and easy to store, convenient to brew, suitable for multiple scenes, through the compounding of probiotics and zinc gluconate and the sugar-free formula, it is suitable for sugar control groups. The application makes full use of the resource advantages of Qaidam Chinese wolfberry, solves the problems of existing product homogenization, poor portability, raw material waste and the like, and the product has both functionality and convenience.

The application provides a ginseng soil novel bacillus, a fermentation method of tobacco leaves, fermented tobacco leaves and a cigarette product. The ginseng soil novel bacillus (Neobacillus ginsengisoli) SCT-B5 has been preserved in the China General Microbiological Culture Collection Center on October 10, 2025, and the address is No. 1, Xibaheyi, Chaoyang District, Beijing, and the preservation number is CGMCC No. 36126. The ginseng soil novel bacillus SCT-B5 has high yields of protease, amylase and cellulase, the protease produced in the fermentation process can reduce the content of protein in tobacco leaves, thereby reducing the irritability and offensive odor of the tobacco leaves; the cellulase can reduce the cellulose content in the tobacco leaves, decompose the cellulose into small molecule sugars, and improve the sweetness and alcohol degree of the smoke.