1299 results about "Cellulase" patented technology

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention relates to the technical field of microorganisms, and particularly discloses a saline-alkaline tolerant bacillus deserticola BW-13 with biocontrol and growth promoting functions and application thereof. The bacillus safensis BW-13 is preserved in the China Center for Type Culture Collection on January 13, 2025, the classification name of the bacillus safensis BW-13 is bacillus safeensis, and the preservation number of the bacillus safensis BW-13 is CCTCC NO: M 2025108. The strain BW-13 has relatively high salt tolerance, and still keeps good resistance under various stress conditions; meanwhile, the plant growth promoting agent has various plant growth promoting characteristics including nitrogen fixation, phosphorus solubilization and cellulase and protease secretion capacity, and pot experiment verifies that the plant growth promoting agent can improve the salt resistance of cucumber seedlings and promote plant growth; the strain has an antagonistic effect on a plurality of phytopathogens, and especially has a remarkable prevention and treatment effect on plant diseases such as apple ring spot, polygonatum kingianum anthracnose, pear tree rot and tomato gray mold caused by ascomycophyta pathogenic fungi.

The invention discloses a multifunctional cellulolytic enzyme and application thereof in degradation of agricultural by-products, and belongs to the technical field of biology. The multifunctional cellulolytic enzyme is named RuCelI, and the amino acid sequence of the multifunctional cellulolytic enzyme is as shown in SEQ ID NO. 1. Based on a rumen microbial metagenome resource mining technology, a novel multifunctional cellulose decomposition enzyme RuCelI is developed, and the enzyme has double activities of endo-cellulase and xylanase, can be used for degrading agricultural by-products, reducing the treatment cost and improving the degradation efficiency, and is beneficial to improving the utilization rate and the economic benefit of the agricultural by-products.

The invention discloses a method for preparing fermentable sugar from recyclable low-concentration p-toluenesulfonic acid pretreated lignocellulose, which comprises the following steps: firstly, pretreating lignocellulose by using a low-concentration p-toluenesulfonic acid aqueous solution, and carrying out solid-liquid separation to obtain hemicellulose hydrolysate and a pretreated solid component; then saccharifying and pretreating the solid component by using cellulase to obtain hydrolysate rich in glucose, adsorbing the hemicellulose hydrolysate by using hypercrosslinked adsorption resin, carrying out solid-liquid separation to obtain hydrolysate rich in xylose, and carrying out desorption treatment on the resin by using industrial ethanol to obtain xylose; and performing rotary evaporation on the desorbed liquid to remove the industrial ethanol to obtain p-toluenesulfonic acid. According to the method, hemicellulose is efficiently degraded to obtain derived sugar of hemicellulose, meanwhile, the saccharification efficiency of cellulase is improved, p-toluenesulfonic acid is recycled through the hypercrosslinked adsorption resin, and the method has the advantages of being high in fermentable sugar yield, recyclable in acid catalyst, low in cost, environmentally friendly and the like.

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.

The invention belongs to the technical field of biological fermentation and cosmetics, and particularly relates to a cortex magnoliae officinalis fermented product with oil-controlling and dandruff-removing effects as well as preparation and application of the cortex magnoliae officinalis fermented product. The preparation method comprises the following steps: performing ethanol ultrasonic extraction on officinal magnolia bark powder to obtain an officinal magnolia bark extract; adding mangnolia officinalis, honeysuckle, green tea and pericarpium citri reticulatae powder into pure water, performing enzymolysis treatment by adopting cellulase and pectinase, then adding a carbon source, prebiotics and inorganic salt, performing high-temperature sterilization, performing cooling, then adding a mangnolia officinalis extract, and performing uniform mixing so as to obtain a fermentation culture medium; the method comprises the following steps: inoculating an aspergillus seed solution and a saccharomyces cerevisiae seed solution into a fermentation culture medium for first-step fermentation, heating and inactivating, cooling, inoculating fruit wine yeast for second-step fermentation, and sterilizing at high temperature, cooling and filtering after fermentation to obtain filtrate which is the cortex magnoliae officinalis fermented product with the effects of controlling oil and removing dandruff. According to the preparation method, enzymolysis and fermentation technologies are combined, and the honeysuckle, the green tea and the pericarpium citri reticulatae are fermented together, so that the antibacterial, anti-dandruff and oil-control effects of the mangnolia officinalis can be synergistically improved.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A118 and application thereof. The bacillus velezensis A118 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on colletotrichum capsici. The bacillus velezensis A118 disclosed by the invention also has relatively strong capability of producing cellulase, amylase, protease and indole-3-acetic acid (IAA) and has a relatively good growth promoting effect on chilies, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in a use process and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of pepper diseases is reduced, the pepper growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention provides a preparation method, application and composition of oat polypeptide, and relates to the technical field of cosmetics. The preparation method disclosed by the invention comprises the following steps: (1) moistening and swelling oat, drying after steam explosion treatment, and adding pectinase, cellulase and amylase to carry out carbohydrate enzymolysis, so as to obtain oat residues after enzymolysis; (2) adding compound protease into the oat residues subjected to enzymolysis, and carrying out primary proteolysis under the condition that the addition amount of ethanol with the volume concentration of 3-8%, so as to obtain enzymolysis slurry A; the compound protease comprises papain, bromelain and flavourzyme in a mass ratio of (3-6): (3-6): 2; (3) adding trypsin into the enzymolysis serous fluid A to carry out secondary proteolysis to obtain enzymolysis serous fluid B; and (4) carrying out ultrafiltration on the enzymolysis slurry B to obtain the oat polypeptide. The obtained oat polypeptide is small in molecular weight and high in content, is used for preparing cosmetics, and has remarkable effects of preserving moisture, resisting wrinkles and reducing skin roughness.

The application discloses a method for improving the quality and moisture content distribution of bean curd by soaking and using water for pulp grinding, and comprises the following steps: (1) pretreatment: accurately weighing CaCl2, MgCl2, KCl and cellulase according to different proportions, dissolving in deionized water, and preparing for use; (2) adjusting pH value: taking sodium bicarbonate, dissolving in the prepared deionized water, and adjusting the pH value; (3) raw material preparation: weighing 500g of yellow beans, pouring into the prepared deionized water in step (2), and soaking for about 8-10h; (4) bean curd preparation: preparing the bean curd product rich in various ions according to the process flow by using the soaked yellow beans. The application has the advantages of simple process, low production cost and suitability for industrial production, and solves the problem that the quality of bean products is different due to different water qualities in different regions for yellow bean processing enterprises.

The invention provides Aspergillus tubingensis HNZY02, a method for producing enzymes by the Aspergillus tubingensis HNZY02 and application of the Aspergillus tubingensis HNZY02, and belongs to the technical field of microorganisms, the Aspergillus tubingensis HNZY02 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.42148. The aspergillus tubingensis HNZY02 shows excellent cellulase and xylanase synthesis capability, can synergistically and efficiently degrade main structural components such as cellulose, hemicellulose and lignin in tobacco stems, and improves the safety and application value of cigarettes while breaking the compact structure and increasing the reducing sugar content.

The invention discloses heavy metal resistant bacillus velezensis as well as a microbial remediation agent and application thereof. Bacillus velezensis DBS01 is screened from heavy metal contaminated soil in Dabaoshan mining areas in Shaoguan City, Guangdong province, is preserved in China Center for Type Culture Collection (CCTCC), has a preservation number of CCTCC M 20252228, not only has resistance to various heavy metals, but also can metabolize and secrete metabolites such as organic acid, cellulase and growth hormone in different growth stages, and can be used for preventing and treating various heavy metals. Multiple functions of curing heavy metals, dissolving phosphorus and potassium, promoting plant growth and the like are realized. According to the method, bacillus velezensis DBS01 is inoculated into a culture medium containing straw, calcium superphosphate, agar and sodium alginate are added, and a mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the heavy metal contaminated soil in the mining area can be efficiently repaired in an environment-friendly manner at low cost, and the ecological function recovery of the soil is effectively promoted.

The present application belongs to the technical field of biological fermentation, and discloses a natural plant fermentation liquor, which is prepared from acanthopanax, angelica sinensis, rosa davurica, phellinus baumii, tea leaves, water, mixed enzymes and mixed bacterial agents; wherein the mixed enzymes include cellulase, pectinase and tannase, and the mixed bacterial agents include lactobacillus plantarum, schizosaccharomyces and lactobacillus acidophilus.The natural plant fermentation liquor has high safety, good clarity after adjusting the concentration of crude drugs, and can be added into a cosmetic matrix.The preparation method is simple, and the effective active ingredients of the natural plants and tea leaves are extracted by the synergistic effect of bacteria and enzymes to the maximum extent.In addition, the present application also discloses the application of the natural plant fermentation liquor in preparing cosmetics for treating oily sensitive skin.

The invention discloses a method for preparing mushroom peptide with salt reducing and salinity increasing capabilities by a controllable biological enzymolysis technology, which comprises the following steps: (1) dissolving edible mushroom powder in water, uniformly stirring, heating and cooling to room temperature; (2) adding cellulase into the solution obtained in the step (1) for enzymolysis, and finishing the first-stage enzymolysis; (3) adding flavourzyme into the enzymatic hydrolysate obtained in the step (2) for enzymolysis, and finishing second-stage enzymolysis; (4) adding alkaline protease into the enzymatic hydrolysate obtained in the step (3) for enzymolysis, and ending third-stage enzymolysis; and (5) carrying out enzyme deactivation on the enzymatic hydrolysate obtained in the step (4), cooling to room temperature, centrifuging, and taking the supernatant to obtain the mushroom protein peptide which is rich in protein peptide and has an obvious salty-increasing effect as the mushroom peptide with salt-reducing and salty-increasing capabilities.

The invention relates to a probiotic fermented fruit and vegetable juice beverage and a preparation method thereof, and belongs to the technical field of food. The invention provides a preparation method of a probiotic fermented fruit and vegetable juice beverage, which mainly comprises the following steps: mixing fruit and vegetable raw materials with deionized water, homogenizing at high pressure, adding polydextrose and resistant starch, stirring for dissolving, sieving, adjusting the pH value, adding pectinase and cellulase, performing enzymolysis, inoculating compound bacteria into enzymatic hydrolysate, performing two-stage fermentation, and obtaining the probiotic fermented fruit and vegetable juice beverage. And adding a fructus momordicae extract into the obtained fermentation liquor, sieving, carrying out pasteurization, immediately cooling to 4 DEG C, filtering, and carrying out sterile filling, thereby obtaining the fructus momordicae health-care wine. The apple, the kale, the jerusalem artichoke, the mandarin orange, the momordica grosvenori extract and the like are compounded to provide multiple trace element supplementation for a human body, lactic acid and short-chain fatty acid are generated in the synergistic double-bacterium fermentation process, and intestinal tract movement is effectively promoted.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention discloses a preparation method of a low-protein biological fermentation feed for crayfish, and belongs to the technical field of aquaculture feeds, and the preparation method comprises the following steps: high-temperature fermentation: uniformly mixing soybean meal, rapeseed meal, cottonseed meal and a mixed bacteria solution containing bacillus subtilis, lactobacillus plantarum, saccharomycetes and EM bacteria, and performing high-temperature fermentation to obtain a fermentation product; and performing low-temperature fermentation: adding shrimp shell meal, corn flour, rice bran, cellulase and phytase into the fermentation product, performing uniform stirring to obtain a mixture, and performing low-temperature fermentation on the mixture to obtain the product. According to the low-protein biological fermentation feed disclosed by the invention, by-products such as shrimp shell meal, rice bran and rapeseed meal are used as raw materials of the biological fermentation feed, so that the cost of the feed can be reduced; and the aims of enhancing the nutritional quality, improving the immunity and oxidation resistance of the organism and improving the environment of a culture water body can be fulfilled for culturing the crayfish with the specification of about 12 grams per crayfish.

The invention relates to a method for extracting and purifying lycium barbarum polyphenol by combining bacteria and enzymes. The method comprises the following steps: pretreating lycium barbarum fruits to obtain lycium barbarum powder; extracting the wolfberry powder by using a first solvent to obtain a first crude extract; fermenting the first crude extract with lactobacillus plantarum to obtain a second crude extract; performing enzymolysis on the second crude extract by using pectinase, hemicellulase and beta-glucosidase in sequence to obtain an enzymolysis extracting solution; adsorbing and purifying the enzymolysis extracting solution by using first ion exchange resin, washing and eluting to obtain a first eluent; adsorbing and purifying the first eluent by using second ion exchange resin, washing and eluting to obtain a second eluent; and concentrating and drying the second eluent to obtain wolfberry polyphenol powder. According to the method disclosed by the invention, the fermentation of lactobacillus plantarum is combined with the synergistic effect of pectinase, hemicellulase and beta-glucosidase, polysaccharide in a crude extract is decomposed, polyphenol is released and combined, the extraction rate and the purity of the polyphenol are improved, and meanwhile, a continuous purification process of macroporous resins AB-8 and D-101 is combined, so that the product quality is further improved.

The invention discloses a method and a system for producing high-purity medium-chain fatty acid (MCFA) based on waste molasses two-stage regulation fermentation. Comprising the following steps: (1) diluting waste molasses, then adding a compound enzyme agent of pectinase, beta-glucanase and cellulase, and coupling microwave to assist treatment; (2) inoculating the pretreated waste molasses with a composite flora containing a clostridium kluyveri mutant strain and a Megasphaera elsdeni engineering bacterium, and carrying out a fermentation treatment on the waste molasses, wherein the composite flora contains the clostridium kluyveri mutant strain and the Megasphaera elsdeni engineering bacterium; (3) sequentially performing two-stage dynamic regulation and control fermentation on the waste molasses inoculated with the composite flora through a pH-stat control strategy to realize efficient conversion from butyric acid to hexanoic acid / octanoic acid; and (4) a hydrophobic hollow fiber membrane module is adopted to realize on-line removal of the product, and the inhibition effect of the product is relieved. According to the technology, the yield of the MCFA produced from the waste molasses reaches 0.45 g / g COD, the cost is reduced by 68% compared with that of a traditional substrate, and a new way is provided for recycling of sugar manufacturing waste.

The invention relates to the technical field of soil amendments, in particular to a soil amendment containing earthworm enzymatic hydrolysate and a preparation method of the soil amendment. The soil conditioner containing the earthworm enzymatic hydrolysate comprises the following components: earthworm enzymatic hydrolysate microcapsules and adjuvant particles in a mass ratio of (2-4): 1, the earthworm enzymatic hydrolysate is prepared by performing enzymolysis on fresh earthworm homogenate by compound enzyme, and the compound enzyme comprises cellulase and protease; the wall material of the earthworm enzymatic hydrolysate microcapsule comprises at least one of chitosan, calcium alginate, gelatin and Arabic gum; the auxiliary agent comprises humic acid and inorganic natural minerals. According to the soil conditioner containing the earthworm enzymatic hydrolysate, the earthworm enzymatic hydrolysate is wrapped by the microcapsules, the retention rate of active ingredients in the earthworm enzymatic hydrolysate can be remarkably increased, and the soil condition can be further improved by matching with humic acid and inorganic natural minerals for use.

The invention relates to a medicine for skin regeneration and repair and a preparation method thereof, and belongs to the technical field of biological medicine, the medicine comprises cyperus esculentus extract peptide, brooklet anemone root tremella extract, rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract, pomegranate peel polyphenol and the like. The cyperus esculentus extracted peptide is a product which is more than 1kDa and is obtained by performing enzymolysis on cyperus esculentus through cellulase, alpha-chymotrypsin and ficin protease in sequence and then performing purification through a G-50 sephadex column; the tremellodon gelatinosum tremella extract is a product which is less than 3kDa and is obtained by performing common enzymolysis on tremellodon gelatinosum tremella sporocarp through cellulase and bromelain; the rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract is a product obtained by performing gradient extraction on rhodomyrtus tomentosa root, suberect spatholobus stem and radix cyathulae through an ethanol water solution to obtain an extract, and performing polyamide resin column adsorption and LH-60 sephadex column purification in sequence. A good repairing system is constructed by multiple components, and wound repairing and healing are accelerated.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A111 and application thereof. The bacillus velezensis A111 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on phytophthora capsici and ralstonia solanacearum. The bacillus velezensis A111 also has relatively strong capability of producing cellulase, amylase, protease, ACC deaminase and indole-3-acetic acid (IAA), and has a relatively good growth promoting effect on tomatoes, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in the use process, and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of tomato diseases is reduced, the tomato growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A96 and application thereof. The bacillus velezensis A96 disclosed by the invention can resist saline and alkaline, and has a good inhibition effect on fusarium oxysporum. In addition, the bacillus velezensis A96 also has relatively strong capacity of producing cellulase, amylase, protease and indole-3-acetic acid (IAA) and has a relatively good growth promoting effect on cowpeas, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in the use process and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the control cost of cowpea diseases is reduced, the growth of cowpeas is promoted, and the biocontrol bacterium has a good popularization value.

Disclosed is a method for reducing the usage amount of edible fat and oil in a bakery product, the method comprising mixing at least one maltose alpha-amylase and edible fat and oil into dough, and baking same to prepare a bakery product. The usage amount of the edible fat and oil in the dough can be reduced by at least 10 wt % compared with not using the enzyme treatment; and the enzyme may also include cellulase and / or phospholipase. The method does not reduce or substantially reduce the quality of the bakery product, and allows same to have a shelf life of at least 4 days. Also involved is baked fat and oil prepared from the above-mentioned enzyme and edible fat and oil.

The invention relates to the technical field of food, in particular to geobacillus stearothermophilus cKM-Geo, application of the geobacillus stearothermophilus cKM-Geo, a shrimp shell oligosaccharide peptide compound and a preparation method of the shrimp shell oligosaccharide peptide compound. The invention provides geobacillus stearothermophilus cKM-Geo, the strain can grow in an oxygen-free environment, ventilation is not needed in the process, and proteolytic enzyme, cellulase, lipase and chitin deacetylase can be highly secreted and expressed; the invention further provides a preparation method for preparing the shrimp shell oligosaccharide peptide by utilizing geobacillus stearothermophilus cKM-Geo fermentation coupling steam explosion treatment, chitin and protein in the shrimp shell are converted into water-soluble oligopeptide and oligosaccharides, the water-soluble oligopeptide and oligosaccharides are separated from calcium carbonate in the shrimp shell, acid and alkali do not need to be used, in the process, waste water is little, pollution is little, and the method is suitable for industrial production. The resource utilization rate is high.

The invention discloses a reconstituted tobacco preparation method based on a compound enzyme technology, which comprises the following steps: carrying out surface impurity removal pretreatment on a reconstituted tobacco sheet base to obtain a standby sheet base; dissolving cellulase and pectinase in water according to a certain mass ratio to prepare an enzyme solution; the preparation method comprises the following steps: spraying a citric acid-disodium hydrogen phosphate buffer solution to the surface of a standby film base, then spraying an enzyme solution to the surface of the standby film base, carrying out an enzymatic hydrolysis reaction for 3-5 h under the conditions that the temperature is 40-50 DEG C and the humidity is 50-70%, and then drying the enzymatic hydrolysis film base for 30-50 min at the temperature of 70-80 DEG C. According to the method, a collaborative regulation system of cellulase and pectinase is constructed, the enzymolysis process is optimized, oriented improvement of the sour and sweet taste of the reconstituted tobacco is achieved, the aroma richness is remarkably enhanced, the overall acceptability is greatly improved, the technical problems that in a traditional process, the flavor is single, and enzymolysis regulation is extensive are solved, and the quality of the reconstituted tobacco is improved. And a green and efficient standardized solution is provided for quality upgrading of the reconstituted tobacco.

The invention discloses a pretreatment method for increasing the yield of anaerobic fermentation methane of a lignocellulose material, and belongs to the technical field of high-efficiency anaerobic fermentation methane production of organic solid waste. The method comprises the following steps: (1) crushing straws; (2) mixing the straw powder and deionized water according to a mass ratio of 1: (19-21), stirring at a rotating speed of 120-180r / min, treating for 22-26h, fully and uniformly mixing, adjusting the pH value of the system to 3.4-3.7, adding 0.08-0.12 wt% of cellulase according to the mass of the straw, and carrying out enzymolysis at 49-51 DEG C for 2-4d to obtain an enzymatic hydrolysate; (3) yeast pre-fermentation: inoculating 4-6% v / v activated angel high-activity dry yeast into the enzymatic hydrolysate in the step (2) for anaerobic pre-fermentation, wherein the pre-fermentation time is 16-20 hours; and (4) anaerobic fermentation for methane production: adding 20-30% v / v of introduction sludge into the fermentation system in the step (3), and carrying out anaerobic fermentation for methane production, wherein the fermentation temperature is 36-38 DEG C. Compared with the prior art, the method has the advantages of being high in adaptability, short in treatment period, good in economic benefit, free of environmental pollution and the like, and has certain guidance and inspiration effects on engineering of producing methane from straw.

The invention relates to the technical field of traditional Chinese medicine effective component extraction, and provides an efficient extraction and purification process and application of icariin in plateau herba epimedii, the efficient extraction and purification process of icariin in plateau herba epimedii comprises the following steps: S1, epimedium leaves are crushed and sieved to obtain epimedium powder; s2, adding an ethanol solution into the herba epimedii powder, then adding a compound enzyme, performing ultrasonic treatment, inactivating the enzyme, performing suction filtration, and collecting filtrate to obtain an icariin crude extract; s3, diluting the crude icariin extract, adjusting the pH value to 5-6, carrying out chromatographic purification and elution, and collecting the eluate; s4, performing vacuum concentration on the eluent, and performing spray drying to obtain a pure icariin product; the compound enzyme comprises cellulase, pectinase and alpha-mannosidase in a mass ratio of 3: 1: (0.2-1.0). By means of the technical scheme, the problem that in the prior art, the extraction rate of icariin in plateau herba epimedii is low is solved.

The invention discloses a method for improving moso bamboo cellulase hydrolysis conversion efficiency through amino acid-based deep eutectic solvent pretreatment, and belongs to the technical field of biomass recycling. The method comprises the following steps: mixing an L-cysteine solid and a formic acid solution, performing water bath heating to prepare an acidic eutectic solvent, performing microwave-assisted eutectic solvent pretreatment on moso bamboos, and performing filtration separation and enzymolysis; wherein the molar ratio of the L-cysteine to the formic acid is 1: (4-5). A deep eutectic solvent formed by formic acid and cysteine has a strong hydrogen bond network and an acid environment, beta-O-4 ether bonds in lignin are promoted to be broken, sulfydryl of cysteine participates in nucleophilic attack, a lignin-carbohydrate complex is further destroyed, formic acid can hydrolyze acetyl and glucosidic bonds in hemicellulose, and the lignin-carbohydrate complex is further destroyed. And degrading into an oligomer or monosaccharide. The phyllostachys pubescens components are efficiently separated, the cellulose enzymolysis saccharification capacity is improved, and effective utilization of all the phyllostachys pubescens components is facilitated.

The invention provides a pitaya wine processing technology. The pitaya wine processing technology is optimized aiming at the problems that pigments are easy to degrade, the fermentation efficiency is low, and the flavor is single in a traditional technology. According to the raw material pretreatment, ultrasonic cleaning (0.05% sodium hexametaphosphate solution) is combined with metal ion control (Fe2 / Cu2 is less than or equal to 0.1 mg / L), and the juice yield is increased through composite enzymolysis (pectinase and cellulase). In the low-temperature fermentation stage, the period is shortened to 10 hours by utilizing fed-batch strain activation, and the retention rate of characteristic aroma is remarkably improved by combining pulse ultrasound and nitrogen protection (the oxygen content is less than or equal to 0.5 ppm); and through infrared monitoring and gradient cooling, accurate control of the alcoholic strength being 12% vol and the residual sugar being less than or equal to 4g / L is realized. In the aging stage, nitrogen is adopted to isolate oxygen (high-purity nitrogen is greater than or equal to 99.99%) and a KPA stabilizer, so that the degradation rate of betacyanin is less than or equal to 10%. Finally, the stability is improved through ceramic membrane filtration, cold stabilization and pasteurization, and the product is purplish red in color and luster and complex in flavor and has industrial application potential.

The invention discloses a preparation method of highland barley prefabricated powder with a low glycemic index and application of the highland barley prefabricated powder in 3D printed biscuits. According to the method, firstly, cellulase and alpha-amylase are adopted for carrying out enzymatic modification treatment on highland barley flour, the content of anti-digestion starch (RS) is remarkably increased, the content of fast-digestion starch (RDS) is reduced, and therefore the modified highland barley flour with the predicted glycemic index (pGI) reaching the low-GI food standard (GI is smaller than or equal to 55) is prepared, and the dietary requirements of people with different metabolic requirements are met. Further, the modified highland barley powder is compounded with sodium alginate, glutamine transaminase is utilized for inducing and constructing a protein-polysaccharide gel network, the incubated and cross-linked dough is subjected to freeze drying and smashing, and the highland barley prefabricated powder with the low glycemic index is obtained.The 3D printing adaptability, the structural stability and the digestion resistance of the material are effectively enhanced through the method. The highland barley biscuit has the characteristic of low glycemic index, and is suitable for making healthy food for special crowds with diabetes and the like.