Strain of biocontrol Streptomyces and application thereof

Abstract

The invention discloses a strain of Streptomyces and application thereof. The biocontrol Streptomyces is blastmycin Streptomyces and its strain number is JZB130180, which is registered in the general microbiological center of China Committee for Culture Collection of Microorganisms with CGMCC No. 13270. The strain had significant antagonistic effect on 14 kinds of plant pathogenic fungi and 6 kinds of plant pathogenic bacteria in agricultural production, and has wide antimicrobial spectrum. The strain produces chitinase, cellulase, protease and selenium, and has the basic characteristics of biocontrol bacteria. The fermentation broth of this strain has strong control effect on tomato gray mold and peach brown rot, and has significant promotion action on the tomato plants, which indicated that the strain had a broad application prospect in the field of biological control of plant diseases.

Description

technical field

[0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a strain of Streptomyces biocontrol and its application. Background technique

[0002] As people pay more and more attention to and understand the concepts of ecological balance, organic food and green health, biological control methods can effectively avoid a series of problems caused by chemical pesticides, and at the same time have profound social benefits and ecological benefits. Benefits and long-term benefits have received extensive and unprecedented attention in agricultural production. Under the background of sustainable development of agricultural science, biological control is also the only way for agricultural development. Actinomycetes are the earliest and most widely used biocontrol microorganisms, and they have played a huge role in the biological control of plant diseases. Actinomycetes are still a research hotspot in the research and deve...

Examples

Embodiment 1

[0102] Example 1, Isolation and Identification of Blastomycin Streptomyces JZB130180

[0103] 1.1 Strain isolation

[0104] Soil samples collected from the high-altitude area of ​​Leigong Mountain in Guizhou Province, China were subjected to dry heat treatment, dried at room temperature for 1-2 weeks, and after being ground and sieved, each soil sample was treated in a blast drying oven at 100°C for 1 hour before to separate. Accurately weigh 1g of the soil sample and put it into a 50ml sterile Erlenmeyer flask filled with 9ml sterile saline solution and small glass beads. -1 Concentration diluent, and then use a 1ml pipette to draw 10 -1 Transfer the concentration diluent into a 9ml sterile Erlenmeyer flask, shake well and serve as 10 -2 Concentration diluent, and so on, diluted to 10 -3 concentrated diluent. After the sample suspension is prepared, use a sterile pipette to draw 10 -2 Concentration and 10 -3 Each 0.1ml of the concentration dilution solution was put int...

Embodiment 2

[0115] Embodiment 2, the bacteriostatic spectrum determination of rice blastomyces streptomyces (Streptomyces blastmyceticus) JZB130180

[0116] Test medium:

[0117] LB agar medium: tryptone 10.0g, yeast extract 5.0g, NaCl 10.0g, agar 15.0g, distilled water 1000mL, pH 7.0~7.2;

[0118] LB liquid medium: except that agar is not added, the others are made with LB agar medium.

[0119] KB medium: peptone 20.0g, glycerol 10.0mL, K 2 HPO 4 1.50g, MgSO 4 ·7H 2 O 1.50g, agar 15.0g, distilled water 1000mL, KOH to adjust the pH value to 7.2;

[0120] All the above-mentioned media were autoclaved at 121°C for 20 minutes.

[0121] experiment method:

[0122] (1) The antibacterial effect on plant pathogenic fungi was determined by the plate confrontation method. The specific operation steps are as follows: first culture the bacterial strain JZB130180 and various plant pathogenic fungi (Table 2) on the PDA medium for 4-5 days respectively, and use a sterile puncher with a diamete...

Embodiment 3

[0129] Example 3, Determination of Biocontrol-related Activity of Bacterial Strain JZB130180

[0130] 1. Test medium: CAS medium (detection of siderophilicity) consists of 4 solutions (sterilized separately before mixing). Solution 1 (CAS / HDTMA solution) includes CAS solution: 60.5mg CAS (chrome azure) dissolved in 50mL water; iron solution: contains 1mmol / L FeCl 3 ·6H 2 10mmol / L HCl solution of O, pH 2.0; HDTMA solution: 72.9mg cetyltrimethylammonium bromide dissolved in 40mL water; mix 50mL CAS solution with 10mL iron solution, add 40mL HDTMA solution and stir well , sterilize the obtained blue-black liquid, which is the CAS / HDTMA solution; solution 2 (Salts / Buffer solution): dissolve 30.24g Pipes in 750mL Salts solution (KH 2 PO 4 0.3g, NaCl0.5g, NH 4 Cl 1.0g), adjust the pH to 6.8 with 50% (W / V) KOH, add 15g of agar and dilute to 800mL with distilled water, cool to 50°C after autoclaving; solution 3 (750mL): glucose 2g, mannitol 2g, MgSO 4 ·7H 2 O 493mg, CaCl 2 11...

technical field

[0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a strain of Streptomyces biocontrol and its application. Background technique

[0002] As people pay more and more attention to and understand the concepts of ecological balance, organic food and green health, biological control methods can effectively avoid a series of problems caused by chemical pesticides, and at the same time have profound social benefits and ecological benefits. Benefits and long-term benefits have received extensive and unprecedented attention in agricultural production. Under the background of sustainable development of agricultural science, biological control is also the only way for agricultural development. Actinomycetes are the earliest and most widely used biocontrol microorganisms, and they have played a huge role in the biological control of plant diseases. Actinomycetes are still a research hotspot in the research and deve...

Examples

Embodiment 1

[0102] Example 1, Isolation and Identification of Blastomycin Streptomyces JZB130180

[0103] 1.1 Strain isolation

[0104] Soil samples collected from the high-altitude area of ​​Leigong Mountain in Guizhou Province, China were subjected to dry heat treatment, dried at room temperature for 1-2 weeks, and after being ground and sieved, each soil sample was treated in a blast drying oven at 100°C for 1 hour before to separate. Accurately weigh 1g of the soil sample and put it into a 50ml sterile Erlenmeyer flask filled with 9ml sterile saline solution and small glass beads. -1 Concentration diluent, and then use a 1ml pipette to draw 10 -1 Transfer the concentration diluent into a 9ml sterile Erlenmeyer flask, shake well and serve as 10 -2 Concentration diluent, and so on, diluted to 10 -3 concentrated diluent. After the sample suspension is prepared, use a sterile pipette to draw 10 -2 Concentration and 10 -3 Each 0.1ml of the concentration dilution solution was put int...

Embodiment 2

[0115] Embodiment 2, the bacteriostatic spectrum determination of rice blastomyces streptomyces (Streptomyces blastmyceticus) JZB130180

[0116] Test medium:

[0117] LB agar medium: tryptone 10.0g, yeast extract 5.0g, NaCl 10.0g, agar 15.0g, distilled water 1000mL, pH 7.0~7.2;

[0118] LB liquid medium: except that agar is not added, the others are made with LB agar medium.

[0119] KB medium: peptone 20.0g, glycerol 10.0mL, K 2 HPO 4 1.50g, MgSO 4 ·7H 2 O 1.50g, agar 15.0g, distilled water 1000mL, KOH to adjust the pH value to 7.2;

[0120] All the above-mentioned media were autoclaved at 121°C for 20 minutes.

[0121] experiment method:

[0122] (1) The antibacterial effect on plant pathogenic fungi was determined by the plate confrontation method. The specific operation steps are as follows: first culture the bacterial strain JZB130180 and various plant pathogenic fungi (Table 2) on the PDA medium for 4-5 days respectively, and use a sterile puncher with a diamete...

Embodiment 3

[0129] Example 3, Determination of Biocontrol-related Activity of Bacterial Strain JZB130180

[0130] 1. Test medium: CAS medium (detection of siderophilicity) consists of 4 solutions (sterilized separately before mixing). Solution 1 (CAS / HDTMA solution) includes CAS solution: 60.5mg CAS (chrome azure) dissolved in 50mL water; iron solution: contains 1mmol / L FeCl 3 ·6H 2 10mmol / L HCl solution of O, pH 2.0; HDTMA solution: 72.9mg cetyltrimethylammonium bromide dissolved in 40mL water; mix 50mL CAS solution with 10mL iron solution, add 40mL HDTMA solution and stir well , sterilize the obtained blue-black liquid, which is the CAS / HDTMA solution; solution 2 (Salts / Buffer solution): dissolve 30.24g Pipes in 750mL Salts solution (KH 2 PO 4 0.3g, NaCl0.5g, NH 4 Cl 1.0g), adjust the pH to 6.8 with 50% (W / V) KOH, add 15g of agar and dilute to 800mL with distilled water, cool to 50°C after autoclaving; solution 3 (750mL): glucose 2g, mannitol 2g, MgSO 4 ·7H 2 O 493mg, CaCl 2 11...

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