531 results about "Agar" patented technology

The invention relates to the technical field of plant growth-promoting bacterium screening, in particular to a thunberg fritillary bulb growth-promoting bacterium colony screening method and system based on image recognition. The screening method comprises the following steps: establishing a three-dimensional gradient dilution system of a thunberg fritillary bulb rhizosphere soil sample, and collecting a bacterial colony original image under a specific wavelength combination by adopting a multispectral imaging device. According to the method, by establishing a multispectral collaborative imaging mechanism and a dynamic growth modeling system, the technical problems of feature dimension deficiency and space-time correlation fracture in a traditional method are effectively solved. An improved wavelet transform algorithm is adopted to break through the optical interference limitation of an agar matrix, and high-fidelity extraction of bacterial colony intrinsic textures is realized; according to the constructed three-dimensional feature space fusion model, morphological topological parameters, texture entropy distribution and growth differential features are deeply coupled through nonlinear mapping, and the biological correlation of feature representation is remarkably improved.

The invention relates to a composition containing camellia nitidissima extract and capable of brightening skin color and application of the composition to preparation of cosmetics suitable for dry sensitive skin, and the composition comprises the following components: camellia nitidissima flower extract, elm bark extract, oat kernel protein and laver polysaccharide in front of water temple, the weight ratio of the camellia nitidissima flower extract to the elm barks extract to the oat kernel protein to the laver polysaccharide in front of water temple is (1-10): (1-8): (0.1-1): (0.01-0.1). The composition can achieve the optimal long-acting brightening, barrier repairing and stimulation relieving effects on the aspect of efficacy.

The invention discloses a mother strain culture medium for boletus xylophilus as well as a preparation method and application of the mother strain culture medium, and belongs to the technical field of microbial culture. The preservation number of the strain JSJ-Bx1 of the boletus sinensis mother strain is CGMCC (China General Microbiological Culture Collection Center) No.42007, and the strain JSJ-Bx1 is preserved in the China General Microbiological Culture Collection Center; the culture medium is prepared from 10-30 g of a carbon source, 2-6 g of a nitrogen source, 1-3 g of inorganic salt, 0.1-0.3 g of a growth factor suitable for the culture medium, 20 g of agar and 1 L of water, and the pH of the culture medium is 4-6. By the adoption of the culture medium, hyphae of the boletus sinensis mother strain grow fast, grow vigorously and are dense, the edges of bacterial colonies are neat, hypha degeneration phenomena such as pigment and water spitting are avoided, and the mother strain culture growth cycle is greatly shortened.

The invention discloses a method for inducing nematode-trapping fungus predatory organ formation, and belongs to the field of applied microorganisms. The method comprises the following specific steps: culturing activated nematode-trapping fungi until spores are generated; washing off the cultured spores, and diluting to obtain a spore suspension with the concentration of 30000-60000 spores / mL; coating the spore suspension into an agar culture medium paved with glass paper, uniformly coating the spore suspension, culturing for 28-36 hours at the temperature of 25-28 DEG C, adding a mixed solution of a predatory organ inducer and water, and continuously culturing for 36-60 hours at the temperature of 25-28 DEG C, so that the nematode-trapping fungi can generate a large number of predatory organs; the predatory organ inducer is one of oleic acid, linoleic acid or pregnenolone. It is found for the first time that a large number of predatory organs can be generated when pregnenolone, oleic acid or linoleic acid is used for inducing nematode-trapping fungi.

The invention discloses a brassica rapa imago feed and a preparation method thereof, and belongs to the technical field of insect breeding. The Eye spot Brassica rapa adult feed comprises 11 to 15 parts of casein, 1 to 3 parts of Webster salt, 4 to 6 parts of cane sugar, 9 to 12 parts of cellulose powder, 0.2 to 0.4 part of L-cysteine, 0.2 to 0.3 part of choline chloride, 0.08 to 0.1 part of inositol, 0.325 to 0.4 part of composite vitamin, 2 to 3 parts of a nutritional ingredient ethanol solution, 12 to 14 parts of a nutritional ingredient aqueous solution, 1 to 2 parts of honey, 2 to 3 parts of agar powder, 0.03 to 0.04 part of cholesterol, 33 to 67 parts of apple jam, 0.3 to 4 parts of royal jelly and 80 to 120 parts of water. In addition, the invention also provides a preparation method of the feed for the Eye spot Brassica rapa imagoes. The feed for the Eye spot Brassica rapa adults can prolong the life of the adults and improve the egg laying amount at the same time.

The invention relates to the technical field of plant extraction, in particular to a preparation method of a plant cell exosome, the plant cell exosome and application of the plant cell exosome. The preparation method comprises the following steps: after the surface of a plant leaf is scratched, inoculating the plant leaf into a first culture medium for induction culture, then transferring the plant leaf into a second culture medium for subculture to obtain a callus, and then adding the callus into a buffer solution for grinding to obtain a filtrate; centrifuging the filtrate again to obtain supernate; and adding an exosome complexing agent into the supernate, incubating overnight, centrifuging, and freeze-drying to obtain the exosome. The first culture medium comprises an MS culture medium and the following components added into the MS culture medium: pullulan, 2, 4-D, agar and NAA; the second culture medium comprises an MS culture medium and the following components added into the MS culture medium: pullulan, 2, 4-D, agar, NAA and sodium phytate. The preparation method can increase the protein content in the exosome.

The invention discloses a dendrobium officinale unmanned inoculation tissue culture method and application thereof. Belongs to the technical field of tissue culture. The vertical gradient culture medium is adopted for the first time, and automatic conversion of the development stage is achieved; a culture medium osmotic pressure gradient design guides directional growth of a root system; according to the nutrition phase change technology, the agar concentration difference of an induction layer, a differentiation layer and a strong seedling layer is utilized, the physical characteristic gradient of a culture medium is achieved, unmanned inoculation tissue culture of dendrobium officinale seeds is achieved through automatic equipment and an optimized tissue culture process, and the problems that in the prior art, artificial inoculation is low in efficiency, high in pollution rate and cost and long in culture period are solved; the efficiency, the quality and the economic benefit of dendrobium officinale tissue culture are improved, and the requirements of large-scale factory production can be met.

The invention discloses heavy metal resistant bacillus velezensis as well as a microbial remediation agent and application thereof. Bacillus velezensis DBS01 is screened from heavy metal contaminated soil in Dabaoshan mining areas in Shaoguan City, Guangdong province, is preserved in China Center for Type Culture Collection (CCTCC), has a preservation number of CCTCC M 20252228, not only has resistance to various heavy metals, but also can metabolize and secrete metabolites such as organic acid, cellulase and growth hormone in different growth stages, and can be used for preventing and treating various heavy metals. Multiple functions of curing heavy metals, dissolving phosphorus and potassium, promoting plant growth and the like are realized. According to the method, bacillus velezensis DBS01 is inoculated into a culture medium containing straw, calcium superphosphate, agar and sodium alginate are added, and a mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the heavy metal contaminated soil in the mining area can be efficiently repaired in an environment-friendly manner at low cost, and the ecological function recovery of the soil is effectively promoted.

The present invention describes a composition, such as a tobacco free or low tobacco nicotine containing composition, comprising: a. From about 0.2 wt % to about 2 wt % nicotine in free base form; b. a native cellulose material in an amount from about 35 wt % to about 60 wt %; c. water in an amount at least 35 wt %; d. one or more pH control salts in an amount from about 0.5 wt % to about 3 wt %; e. a release control agent comprising agar agar in an amount from about 0.1 wt % to about 2 wt % f. optional additional ingredients, preferably in an amount up to about 12 wt %. The composition may be provided in pouches for oral delivery to a user.

A method for the bioremediation of hydrocarbon-contaminated production water through the microbiological process of culturing salt-tolerant bacteria in a high-salt cell culture medium for decontamination. The high-salt cell culture medium contains sodium chloride, sodium sulfate, sodium bicarbonate, calcium chloride, and calcium chloride, along with agar, water, and salt-tolerant bacteria to form broth. Once incubated to form an inoculation mixture, this broth is combined with production water to produce a bacteria-containing liquid mixture, and then treated with production water, bioremediating by propagating the bacteria-containing liquid mixture in the treated production water.

This invention discloses a Chinese saprophytic bolete ( Buchwaldoboletus xylophilus This invention relates to the mother culture medium, its preparation method, and its application, belonging to the field of microbial culture technology. The strain JSJ-Bx1 of the *Boletus spp.* mother culture, with accession number CGMCC No. 42007, is deposited at the China General Microbiological Culture Collection Center. The culture medium composition is: 10–30 g carbon source, 2–6 g nitrogen source, 1–3 g inorganic salts, 0.1–0.3 g suitable growth factors, 20 g agar, and 1 L water, with a pH of 4–6. Using the culture medium of this invention, the mycelium of the *Boletus spp.* mother culture exhibits rapid, vigorous, and dense growth, with neat colony edges, and does not produce pigments or exhibit mycelial degeneration such as exudation, significantly shortening the mother culture growth cycle.

The invention provides agaro-oligosaccharide as well as a preparation method and application thereof. The agaro-oligosaccharide is obtained by carrying out enzymolysis on a strain and agar; the bacterial strain comprises at least one of flavobacterium galactivorans and phycophagella albicans. The main component of the agaro-oligosaccharide is neoagarotetraose, and the agaro-oligosaccharide has lower polymerization degree, so that the agaro-oligosaccharide can be more easily absorbed by a human body.

The invention discloses a pH and pectinase dual-response type pesticide microcapsule and a preparation method and application thereof.The pH and pectinase dual-response type pesticide microcapsule is of a core-shell structure, the core structure is prochloraz ionic liquid, and the shell structure sequentially comprises a metal-phenol network layer and a pectin layer from inside to outside; and the metal-phenol network layer is a tannic acid-copper network layer. In order to further strengthen long-term autonomous management of plant diseases and reduce the problem of pesticide leakage in a traditional spraying method to the maximum extent, the invention further provides an adhesive gel patch, agar hydrogel is used for stabilizing pesticide microcapsules, silicone rubber is used as a protective layer, and stable attachment of the patch to leaves is achieved by means of magnets. The preparation process of the pH and pectinase double-response type pesticide microcapsule and the adhesive gel patch thereof has the advantages of rapidness, economy, environmental protection and suitability for industrial production, and has huge potential in sustainable management of plant diseases and precision agriculture.

The invention discloses a long lysinibacillus inoculant, a preparation method and application, the inoculant is prepared from a long lysinibacillus strain C1, and the preparation method comprises the following steps: culturing the strain of the long lysinibacillus strain through a tryptone soybean agar culture medium until yellow single colonies appear; inoculating the yellow single colony into serum, and carrying out seed culture by using a long lysine bacillus liquid culture medium until a logarithmic phase to obtain a bacterial solution; and inoculating the cultured bacterial liquid into a production device according to a long lysine bacillus strain, and carrying out production culture by using a long lysine bacillus production culture medium to a logarithmic phase to prepare the long lysine bacillus biocontrol inoculant. And carrying out centrifugal treatment on the obtained biocontrol microbial inoculum of the long lysine bacillus, taking a supernatant, and filtering the supernatant to obtain the long lysine bacillus biocontrol fermentation broth. The invention further discloses application of the lysinibacillus elongate microbial inoculum in antagonism of rice blast bacteria and prevention and control of rice blast.

The invention relates to the field of agricultural seedling raising, and discloses a net shed seedling raising substrate and a preparation method and application thereof, the net shed seedling raising substrate comprises a modified peat-attapulgite composite material, perlite, a water-absorbing gel, a complex microbial inoculant and a fertilizer component; the modified peat-attapulgite composite material is prepared by compounding peat and attapulgite in an acid leaching manner by using a hydrochloric acid solution; the water-absorbing gel is prepared by forming a double-layer network structure by using aminated diatomite, an aminated water-absorbing polymer, sodium alginate, an initiator, a cross-linking agent, an accelerator, calcium bentonite and a calcium chloride solution, the aminated diatomite is prepared by modifying by using dopamine, and the aminated water-absorbing polymer is prepared by using polyvinyl alcohol and agar as polymeric monomers. According to the invention, the water-absorbing gel and the modified peat-attapulgite composite material are added into the seedling culture substrate, so that the conductivity can be reduced, the maximum water-holding capacity is improved, and the cultured plant has thick stems and strong seedlings.

The invention relates to the field of soil remediation, and particularly discloses a fertilizer synergist with a soil remediation effect and a preparation method of the fertilizer synergist. The invention discloses a fertilizer synergist with a soil remediation effect. The fertilizer synergist comprises the following raw materials in percentage by weight: 30-50% of an organic matter modifier, 5-10% of a microelement chelating agent, 10-20% of a biostimulant, 5-10% of a beneficial microbial agent and the balance of a filler, the biostimulant is a seaweed extract, and the seaweed extract is at least one of carrageenan, sodium alginate, propylene glycol alginate and agar; through the synergistic effect of a multi-component formula, the fertilizer synergist is promoted to effectively deal with the problems of soil heavy metal pollution, salinization and organic matter loss, the soil structure is improved, and the product quality is improved.

The invention belongs to the technical field of insect feeding, and particularly relates to artificial feed for spodoptera frugiperda. Comprising the following raw materials in parts by mass: 10 to 30 parts of soybean meal, 30 to 50 parts of wheat germ, 70 to 100 parts of wheat bran, 10 to 50 parts of corn flour, 20 to 50 parts of crushed corn leaves, 15 to 40 parts of beer yeast powder, 5 to 30 parts of five-vitamin glucose, 5 to 20 parts of casein, 5 to 15 parts of ascorbic acid, 25 to 35 parts of agar powder, 0.5 to 2.0 parts of choline, 1.0 to 3.0 parts of sorbic acid, 0.1 to 0.5 part of inositol, 0.05 to 0.20 part of streptomycin sulfate, 1.0 to 3.0 parts of methyl parahydroxybenzoate, 15 to 30 parts of konjaku flour, 15 to 35 parts of milk powder, 0.2 to 2.0 parts of vitamin B6 and 1.0 to 1.0 part of corn germ oil. 5.0 parts of water and 1000 to 1500 parts of water. The artificial feed can significantly shorten the developmental duration of spodoptera frugiperda and improve the pupation rate and eclosion rate.

The present invention discloses a pet food containing fish-derived raw materials with added vitamins and having the efficacy of beautifying hair and skin care, and a preparation method thereof. The pet food containing fish-derived raw materials with added vitamins of the present invention comprises the following raw materials in parts by weight: 8-16 parts of salmon, 1-5 parts of salmon fish oil, 50-60 parts of chicken, 10-15 parts of chicken liver, 10-15 parts of chicken heart, 2-5 parts of sweet potato powder, 1-5 parts of brewer's yeast powder, 2-5 parts of chicken oil, 1-2 parts of psyllium powder, 1-2 parts of chicory root powder, 1-2 parts of cellulose, 0.5-2 parts of egg yolk lecithin, 0.2-0.8 parts of polyglycerol fatty acid ester, 5-20 parts of water, 0.2-0.5 parts of vitamin E, and 0.05-0.1 parts of agar. The pet food containing fish-derived raw materials with added vitamins of the present invention is nutritionally comprehensive, can enhance the physique of pets, help the growth of pet hair, and make the pet hair color bright and shiny.

The invention provides a teak camel moth artificial feeding feed and a teak camel moth artificial feeding method, the feed comprises the following components by weight: 8% of wheat germ powder, 4% of soybean meal, 4% of yeast powder, 2% of casein, 2% of sucrose, 1.8% of agar powder, 1.5% of cellulose, 0.8% of Webster salt, 0.6% of vitamin C, 0.2% of sorbic acid, 0.3% of methyl p-hydroxybenzoate, 0.1% of cholesterol, 0.1% of choline chloride, 0.05% of inositol, 0.01% of folic acid, and the balance of water. And the balance of deionized water. The invention further provides an artificial feeding method of teak camelmoths, and the artificial feeding method comprises the steps of 1, adult mating and egg laying, 2, egg disinfection and incubation and 3, larva rearing. The feed does not contain host material teak leaves, and full-artificial continuous large-scale feeding of the teak camelmoths can be achieved.

The invention belongs to the technical field of in-situ analysis of water environment interface nutrient elements ammonia nitrogen and nitrate nitrogen, and particularly relates to a zeolite and S110MH mixed adsorption film and a preparation method and application thereof. The zeolite and S110MH mixed adsorption film provided by the invention comprises agar hydrogel and adsorbent particles dispersed in the agar hydrogel, and the adsorbent particles comprise zeolite particles and anion exchange resin particles. The invention provides a preparation method of a zeolite and S110MH mixed adsorption film, functional parameters of the mixed adsorption film and a DGT device installed on the mixed adsorption film, and a testing method of DGT in various environmental media. Experiments show that the DGT device provided with the zeolite and S110MH mixed adsorption film can accurately test the time average concentration or diffusion flux of ammonia nitrogen and nitrate nitrogen at rhizosphere of a water body, sediment or aquatic plants, and reflect the bio-availability, geochemical process and mobility of inorganic nitrogen.

The method comprises the following steps: S1, preparation of a mother strain culture medium: firstly, cleaning potatoes, peeling, cutting into blocks, adding water, boiling for 30 minutes, and filtering to remove the potatoes; adding agar powder, continuously decocting until boiling, then sequentially adding monopotassium phosphate, magnesium sulfate, peptone and VB1, uniformly stirring, decocting until boiling, turning off fire, cooling, adding glucose, supplementing water to 1L, sub-packaging, sterilizing at high temperature, taking out, and cooling to obtain a mother strain culture medium; and S2, obtaining the isolated species of the original strains of the sporotrichum fulvum: collecting the original strains of the sporotrichum fulvum, disinfecting, and placing on an inoculation workbench. According to the method, fungi capable of rapidly promoting germination of the cremastra appendiculata seeds form associated fungi suitable for germination of the cremastra appendiculata seeds after being cultured, cremastra appendiculata seedlings can be bred in a large scale in a short time through a specific seedling raising technology, the requirement of the market for cremastra appendiculata planting is met, and the market potential is huge.

The invention relates to the technical field of culture media, and particularly discloses a candida chromogenic culture medium as well as a preparation method and application thereof. The candida chromogenic culture medium disclosed by the invention is prepared from the following components in concentration: 10 to 11 g / L of peptone, 12 to 17 g / L of agar, 7 to 9 g / L of glucose, 1 to 2 g / L of chromogenic substrate and 0.4 to 0.6 g / L of bacteriostatic agent, the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D-galactosamine and 4-methylumbelliferone-beta-D-N-acetyl galactosamine, and the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D- The bacteriostatic agent is prepared by mixing chloramphenicol, taurocholic acid and nalidixic acid. When being used for detecting and identifying candida albicans, candida tropicalis, candida krusei and candida glabrata, the chromogenic culture medium provided by the invention has strong specificity, short identification time and wide practical application value.

The invention discloses a feed for feeding cordyceps sinensis host hepialus larvae as well as a preparation method and application of the feed, and relates to the technical field of artificial feeds. The feed comprises the following components in parts by weight: 5-20 parts of beer yeast powder and / or casein, 30-80 parts of wheat germ powder, 20-40 parts of carrot powder, 0.2-5 parts of a modified preservative, 0.05-0.2 part of antibiotics, 0.2-5 parts of vitamins, 0.01-1 part of edible oil, 6-15 parts of agar and 200-400 parts of water. The modified preservative is composed of a chitosan oligosaccharide-natamycin compound subjected to plasma activation treatment and potassium sorbate according to the ratio of 1: 3-1: 5; a modified preservative composed of a chitosan oligosaccharide-natamycin compound subjected to plasma activation treatment and potassium sorbate is adopted, a porous microsphere structure is formed through a specific preparation process, and the contact area of the preservative and the feed is greatly increased. Agar and water form a gel matrix at a specific temperature, and nutritional ingredients and the like are injected into a coaxial micro-fluidic device, so that the preservative effect of the feed is remarkably enhanced.

The method comprises the following steps: (1) peeling seeds, disinfecting the peeled seeds with 75% ethanol and 10% NaClO, and inoculating the disinfected seeds to a hormone-free MS culture medium; (2) after the seeds germinate, cutting a hypocotyl which grows robustly, and cutting the hypocotyl into sections of 1cm as explants; (3) inoculating the hypocotyl to an induction medium (MS + 1.5 mg / L 6-BA + 1.5 mg / L 2, 4-D + 0.5 mg / L NAA + 30 g / L cane sugar + 7 g / L agar with pH of 6.2), and culturing for 30 days under the conditions of 25 + / -2 DEG C and illumination for 12 h / d to obtain a white green callus with a good state, and the induction rate reaches 71.1%; and (4) transferring the callus to a proliferation culture medium (MS + 1.5 mg / L 6-BA + 0.5 mg / L NAA + 30g / L sucrose + 7g / L agar, pH being 6.2), and continuously culturing for 30 days to realize expansion proliferation of the callus. The method is easy and convenient to operate and good in repeatability, and an effective way is provided for rapid propagation, germplasm preservation and secondary metabolite production of the rheum himalayana.

The invention discloses a method for improving the biomass and flavone content of a saussurea involucrata cell culture, which comprises the following steps of: culturing yellow-white saussurea involucrata callus on a culture medium at the temperature of 24 DEG C, illuminating for 16 hours / day, subculturing once every 15 days and subculturing for three times to obtain yellow-white or yellow-green callus which grows vigorously and is compact in structure; the preparation method of the culture medium comprises the following steps: weighing MS, cane sugar, naphthylacetic acid, 6-benzyladenine, coconut extract and agar in a container, adding distilled water, fully stirring, fixing the volume to 1 L, uniformly stirring, and adjusting the pH value to obtain a basic culture solution; carrying out high-pressure sterilization on the basic culture solution, pouring a flat plate, and cooling to obtain a culture medium; wherein the coconut extract is one or two of coconut water or coconut milk. By adding coconut water or coconut milk, the biomass of the saussurea involucrata callus can be remarkably increased in cooperation with the MS culture medium, and compared with an existing report, the increasing multiple is 2-3 times that of the existing report.

The invention provides termite bait with good palatability, a preparation method and an intelligent termite monitoring device. The termite bait is prepared from the following raw materials in parts by weight: 5-60 parts of basidiomycetes culture and 40-95 parts of microcrystalline cellulose, wherein the basidiomycetes culture is prepared by the following method: fully mixing potato powder, glucose, agar and water according to the mass ratio of 50: 20: 20: (800-950), sterilizing and cooling at high temperature, inoculating basidiomycetes strains, culturing for 20 days at the temperature of 24 DEG C and the air humidity of 60-70%, drying and crushing to obtain the basidiomycetes culture. According to the termite bait prepared from the basidiomycetes culture, test data shows that the termite bait has a very good attraction effect on civil amphibious termites belonging to Coptotermes formosanus and Retilitermes speratus and also has a very good attraction effect on soil-dwelling termites belonging to Coptotermes speratus and Oodontotermes formosanus; therefore, the termite bait disclosed by the invention is excellent in palatability and free from repellency.

The invention discloses a culture medium for identifying gardnerella vaginalis, and relates to the technical field of bacterial culture media, the key points of the technical scheme are as follows: the culture medium comprises the following raw material components by concentration: 10.0 g / L of casein pancreatin digest, 3.0 g / L of cardiac pancreatin digest, 5.0 g / L of yeast extract powder, 1.0 g / L of corn starch, 5.0 g / L of sodium chloride and 13.0 g / L of agar, and the final concentration of defiberized sheep blood is 5%. The pharmaceutical composition is prepared from 1 mg / mL of sodium alginate, 1 mg / mL of sodium alginate, 1 mg / mL of polyanethole Sulfonate (SPS) and 10 [mu] g / mL of vancomycin. According to the invention, through optimal combination of specific components and dosages thereof, rapid identification and screening of gardnerella vaginalis can be realized, and the method has the advantages of accurate result, strong specificity and the like, and has practical popularization and application values.

The present application relates to the technical field of plant pathology, and particularly relates to a method for artificially inducing large sporulation of Ascochyta aspidii, comprising the following steps: S1, strain activation: inoculating Ascochyta aspidii into PDA culture medium, and culturing under light at 20 DEG C for 7 days; S2, sporulation induction: inoculating the activated Ascochyta aspidii into an optimized sporulation culture medium, culturing under near-ultraviolet light at 20 DEG C for 14 days, and obtaining a large amount of spores, wherein the components of the optimized sporulation culture medium include 200 mL / L V8 juice, 0.3 g / L CaCO3, 1 g / L glucose, 1 g / L potassium nitrate, and 15-20 g / L agar. The present application establishes a rapid, efficient and simple in-vitro sporulation method of Ascochyta aspidii, which can effectively solve the technical problem of difficult sporulation under conventional culture conditions in a laboratory, and provides sufficient experimental materials for in-depth analysis of the pathogenic mechanism.

The invention discloses method for manufacturing agar or agarose beads, comprising the steps of:a) providing a water phase comprising an aqueous solution of agar or agarose at a temperature of 40-100° C.;b) providing an oil phase comprising a water-immiscible solvent and an emulsifier at a temperature of 40-100° C.;c) emulsifying the water phase in the oil phase to form a water-in-oil emulsion;d) cooling the water-in-oil emulsion to a temperature below a gelation temperature of the agar or agarose to form a dispersion of solidified agar or agarose beads; ande) recovering agar or agarose beads from the dispersion,wherein the emulsifier comprises a phosphate ester of an alkoxylated fatty alcohol.

The invention discloses a ZSM-48 molecular sieve preparation method, which comprises: (1) adding agar into water, heating in an oil bath at a temperature of 105-120 DEG C to completely dissolve the agar so as to form an agar solution, and cooling the agar solution to a temperature of 70-80 DEG C; (2) adding a silicon source, a template agent, absolute ethyl alcohol and a first alkali source into the agar solution, and stirring at 70-80 DEG C to form a suspension containing microcapsules; and (3) adding an aluminum source and a second alkali source into the suspension, uniformly stirring to form a reaction solution, and then aging, crystallizing, filtering, washing and roasting to obtain the ZSM-48 molecular sieve. The invention also discloses a ZSM-48 molecular sieve supported metal catalyst prepared by adopting the ZSM-48 molecular sieve and application of the catalyst in a hydroisomerization reaction. The agar is used for dispersing the reaction materials to limit the reaction speed and improve the particle size and dispersity of the molecular sieve crystal grains, agglomeration of the crystal grains is avoided, and more acid sites can be released.