367 results about "Agar" patented technology

The invention discloses heavy metal resistant bacillus velezensis as well as a microbial remediation agent and application thereof. Bacillus velezensis DBS01 is screened from heavy metal contaminated soil in Dabaoshan mining areas in Shaoguan City, Guangdong province, is preserved in China Center for Type Culture Collection (CCTCC), has a preservation number of CCTCC M 20252228, not only has resistance to various heavy metals, but also can metabolize and secrete metabolites such as organic acid, cellulase and growth hormone in different growth stages, and can be used for preventing and treating various heavy metals. Multiple functions of curing heavy metals, dissolving phosphorus and potassium, promoting plant growth and the like are realized. According to the method, bacillus velezensis DBS01 is inoculated into a culture medium containing straw, calcium superphosphate, agar and sodium alginate are added, and a mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the heavy metal contaminated soil in the mining area can be efficiently repaired in an environment-friendly manner at low cost, and the ecological function recovery of the soil is effectively promoted.

A method for the bioremediation of hydrocarbon-contaminated production water through the microbiological process of culturing salt-tolerant bacteria in a high-salt cell culture medium for decontamination. The high-salt cell culture medium contains sodium chloride, sodium sulfate, sodium bicarbonate, calcium chloride, and calcium chloride, along with agar, water, and salt-tolerant bacteria to form broth. Once incubated to form an inoculation mixture, this broth is combined with production water to produce a bacteria-containing liquid mixture, and then treated with production water, bioremediating by propagating the bacteria-containing liquid mixture in the treated production water.

This invention discloses a Chinese saprophytic bolete ( Buchwaldoboletus xylophilus This invention relates to the mother culture medium, its preparation method, and its application, belonging to the field of microbial culture technology. The strain JSJ-Bx1 of the *Boletus spp.* mother culture, with accession number CGMCC No. 42007, is deposited at the China General Microbiological Culture Collection Center. The culture medium composition is: 10–30 g carbon source, 2–6 g nitrogen source, 1–3 g inorganic salts, 0.1–0.3 g suitable growth factors, 20 g agar, and 1 L water, with a pH of 4–6. Using the culture medium of this invention, the mycelium of the *Boletus spp.* mother culture exhibits rapid, vigorous, and dense growth, with neat colony edges, and does not produce pigments or exhibit mycelial degeneration such as exudation, significantly shortening the mother culture growth cycle.

The invention provides a teak camel moth artificial feeding feed and a teak camel moth artificial feeding method, the feed comprises the following components by weight: 8% of wheat germ powder, 4% of soybean meal, 4% of yeast powder, 2% of casein, 2% of sucrose, 1.8% of agar powder, 1.5% of cellulose, 0.8% of Webster salt, 0.6% of vitamin C, 0.2% of sorbic acid, 0.3% of methyl p-hydroxybenzoate, 0.1% of cholesterol, 0.1% of choline chloride, 0.05% of inositol, 0.01% of folic acid, and the balance of water. And the balance of deionized water. The invention further provides an artificial feeding method of teak camelmoths, and the artificial feeding method comprises the steps of 1, adult mating and egg laying, 2, egg disinfection and incubation and 3, larva rearing. The feed does not contain host material teak leaves, and full-artificial continuous large-scale feeding of the teak camelmoths can be achieved.

The invention belongs to the technical field of in-situ analysis of water environment interface nutrient elements ammonia nitrogen and nitrate nitrogen, and particularly relates to a zeolite and S110MH mixed adsorption film and a preparation method and application thereof. The zeolite and S110MH mixed adsorption film provided by the invention comprises agar hydrogel and adsorbent particles dispersed in the agar hydrogel, and the adsorbent particles comprise zeolite particles and anion exchange resin particles. The invention provides a preparation method of a zeolite and S110MH mixed adsorption film, functional parameters of the mixed adsorption film and a DGT device installed on the mixed adsorption film, and a testing method of DGT in various environmental media. Experiments show that the DGT device provided with the zeolite and S110MH mixed adsorption film can accurately test the time average concentration or diffusion flux of ammonia nitrogen and nitrate nitrogen at rhizosphere of a water body, sediment or aquatic plants, and reflect the bio-availability, geochemical process and mobility of inorganic nitrogen.

The invention relates to the technical field of culture media, and particularly discloses a candida chromogenic culture medium as well as a preparation method and application thereof. The candida chromogenic culture medium disclosed by the invention is prepared from the following components in concentration: 10 to 11 g / L of peptone, 12 to 17 g / L of agar, 7 to 9 g / L of glucose, 1 to 2 g / L of chromogenic substrate and 0.4 to 0.6 g / L of bacteriostatic agent, the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D-galactosamine and 4-methylumbelliferone-beta-D-N-acetyl galactosamine, and the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D- The bacteriostatic agent is prepared by mixing chloramphenicol, taurocholic acid and nalidixic acid. When being used for detecting and identifying candida albicans, candida tropicalis, candida krusei and candida glabrata, the chromogenic culture medium provided by the invention has strong specificity, short identification time and wide practical application value.

The method comprises the following steps: (1) peeling seeds, disinfecting the peeled seeds with 75% ethanol and 10% NaClO, and inoculating the disinfected seeds to a hormone-free MS culture medium; (2) after the seeds germinate, cutting a hypocotyl which grows robustly, and cutting the hypocotyl into sections of 1cm as explants; (3) inoculating the hypocotyl to an induction medium (MS + 1.5 mg / L 6-BA + 1.5 mg / L 2, 4-D + 0.5 mg / L NAA + 30 g / L cane sugar + 7 g / L agar with pH of 6.2), and culturing for 30 days under the conditions of 25 + / -2 DEG C and illumination for 12 h / d to obtain a white green callus with a good state, and the induction rate reaches 71.1%; and (4) transferring the callus to a proliferation culture medium (MS + 1.5 mg / L 6-BA + 0.5 mg / L NAA + 30g / L sucrose + 7g / L agar, pH being 6.2), and continuously culturing for 30 days to realize expansion proliferation of the callus. The method is easy and convenient to operate and good in repeatability, and an effective way is provided for rapid propagation, germplasm preservation and secondary metabolite production of the rheum himalayana.

The invention discloses a method for improving the biomass and flavone content of a saussurea involucrata cell culture, which comprises the following steps of: culturing yellow-white saussurea involucrata callus on a culture medium at the temperature of 24 DEG C, illuminating for 16 hours / day, subculturing once every 15 days and subculturing for three times to obtain yellow-white or yellow-green callus which grows vigorously and is compact in structure; the preparation method of the culture medium comprises the following steps: weighing MS, cane sugar, naphthylacetic acid, 6-benzyladenine, coconut extract and agar in a container, adding distilled water, fully stirring, fixing the volume to 1 L, uniformly stirring, and adjusting the pH value to obtain a basic culture solution; carrying out high-pressure sterilization on the basic culture solution, pouring a flat plate, and cooling to obtain a culture medium; wherein the coconut extract is one or two of coconut water or coconut milk. By adding coconut water or coconut milk, the biomass of the saussurea involucrata callus can be remarkably increased in cooperation with the MS culture medium, and compared with an existing report, the increasing multiple is 2-3 times that of the existing report.

The invention discloses a culture medium for identifying gardnerella vaginalis, and relates to the technical field of bacterial culture media, the key points of the technical scheme are as follows: the culture medium comprises the following raw material components by concentration: 10.0 g / L of casein pancreatin digest, 3.0 g / L of cardiac pancreatin digest, 5.0 g / L of yeast extract powder, 1.0 g / L of corn starch, 5.0 g / L of sodium chloride and 13.0 g / L of agar, and the final concentration of defiberized sheep blood is 5%. The pharmaceutical composition is prepared from 1 mg / mL of sodium alginate, 1 mg / mL of sodium alginate, 1 mg / mL of polyanethole Sulfonate (SPS) and 10 [mu] g / mL of vancomycin. According to the invention, through optimal combination of specific components and dosages thereof, rapid identification and screening of gardnerella vaginalis can be realized, and the method has the advantages of accurate result, strong specificity and the like, and has practical popularization and application values.

The present application relates to the technical field of plant pathology, and particularly relates to a method for artificially inducing large sporulation of Ascochyta aspidii, comprising the following steps: S1, strain activation: inoculating Ascochyta aspidii into PDA culture medium, and culturing under light at 20 DEG C for 7 days; S2, sporulation induction: inoculating the activated Ascochyta aspidii into an optimized sporulation culture medium, culturing under near-ultraviolet light at 20 DEG C for 14 days, and obtaining a large amount of spores, wherein the components of the optimized sporulation culture medium include 200 mL / L V8 juice, 0.3 g / L CaCO3, 1 g / L glucose, 1 g / L potassium nitrate, and 15-20 g / L agar. The present application establishes a rapid, efficient and simple in-vitro sporulation method of Ascochyta aspidii, which can effectively solve the technical problem of difficult sporulation under conventional culture conditions in a laboratory, and provides sufficient experimental materials for in-depth analysis of the pathogenic mechanism.

The invention discloses a ZSM-48 molecular sieve preparation method, which comprises: (1) adding agar into water, heating in an oil bath at a temperature of 105-120 DEG C to completely dissolve the agar so as to form an agar solution, and cooling the agar solution to a temperature of 70-80 DEG C; (2) adding a silicon source, a template agent, absolute ethyl alcohol and a first alkali source into the agar solution, and stirring at 70-80 DEG C to form a suspension containing microcapsules; and (3) adding an aluminum source and a second alkali source into the suspension, uniformly stirring to form a reaction solution, and then aging, crystallizing, filtering, washing and roasting to obtain the ZSM-48 molecular sieve. The invention also discloses a ZSM-48 molecular sieve supported metal catalyst prepared by adopting the ZSM-48 molecular sieve and application of the catalyst in a hydroisomerization reaction. The agar is used for dispersing the reaction materials to limit the reaction speed and improve the particle size and dispersity of the molecular sieve crystal grains, agglomeration of the crystal grains is avoided, and more acid sites can be released.

The invention relates to a microbial biofilm cultivation method comprising the use of a soft agar hydrogel substrate and one or more stains for detection and / or quantification of one or more microbial components and / or characteristics. The invention further relates to the use of the microbial biofilm cultivation method for screening of one or more antimicrobial compounds.

The utility model discloses a kind of observation devices for in-situ evaluation crop absorption fertilizer nutrient, from below to above sequentially include solution collection layer, agar layer, soil layer and gel layer;Wherein soil layer is inserted with first ion concentration sensor, solution collection layer is inserted with second ion concentration sensor.The utility model directly determines nutrient concentration (such as potassium, nitrogen ion) in soil layer and solution collection layer by ion concentration sensor, compared with the mode of traditional determination nutrient concentration, without destroying crop or harvesting plant, realize dynamic, continuous in-situ evaluation.Determination nutrient content in soil extract (not direct soil), avoid the error caused by traditional method because of soil uneven, significantly improve data accuracy.

The invention discloses an artificial feed for feeding purple-stem beetle larvae as well as a preparation method and application of the artificial feed, and belongs to the technical field of insect feeds. The feed takes kudzu vine powder as a core basic component (20-40 parts by weight), and wheat germ powder, soybean meal, yeast powder, cane sugar, agar, Webster salt, a preservative, cholesterol and water as auxiliary materials. The preparation method comprises the steps of dry material mixing, agar hot melting and mixing, high-pressure sterilization, cooling, preservative adding and solidification forming. The feed successfully simulates a natural food source of the purple-stem beetle larvae for the first time, is good in palatability and comprehensive in nutrition, and can support the larvae to efficiently complete growth and development and successfully pupate, and the survival rate can reach 80% or above. According to the method, the bottleneck that the culture of the callicarpa purpurea larvae depends on the fresh kudzu vine is thoroughly solved, and a core material guarantee is provided for standardized, annual and large-scale artificial propagation of the callicarpa purpurea larvae.

The invention discloses recombinant poria cocos chitin endonuclease as well as a gene, a preparation method and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO.1 in a sequence table. According to the nucleotide sequence as shown in SEQ ID NO.1, high-level recombinant secretory expression of target protein in pichia pastoris host bacteria can be realized by taking pichia pastoris inducible expression plasmid pPICZ alpha A as a carrier. Under a shake flask fermentation condition, the average secretory expression quantity can reach 300.3 mg / L. The recombinant enzyme obtained by nickel ion affinity chromatography purification not only can effectively hydrolyze colloidal chitin to generate chitosan oligosaccharide (COS), but also can directly degrade chitin-containing raw materials such as shrimps, crabs and the like to generate COS. In addition, the enzyme also shows the activity of hydrolyzing polysaccharides such as cellulose, pectin, agar and the like, and has important potential values in the fields of functional chitosan oligosaccharide preparation, biological energy production, medicine development, feed, environmental protection, agricultural application and the like.

This invention provides an induction culture medium and method for using marigold anthers as explants. The induction culture medium is based on MS solid medium containing agar, with specific amounts of sucrose, tryptophan, phenylalanine, arginine, casein amino acids, anhydrous asparagine, 6-benzylpurine, naphthaleneacetic acid, silver nitrate, pyridoxine hydrochloride, thiamine hydrochloride, nicotinic acid, D-biotin, folic acid, ascorbic acid, and coconut juice added. This invention also provides a method for induction culture using this induction culture medium. This invention can greatly improve the callus induction rate of marigolds, significantly increasing the number of regenerated seedlings, and provides technical guidance for the cultivation of haploid marigolds using anthers.

The application discloses a chromogenic culture medium for detecting enteropathogenic Escherichia coli, which contains peptone, sodium pyruvate, sodium chloride, D-sorbitol, D-malic acid, bile salt, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium hydroxide, kaolin, bromothymol blue, crystal violet, polyethylene glycol and agar, and the rest is water. The chromogenic culture medium is simple in composition, convenient to prepare and use, and can be used for identifying enteropathogenic Escherichia coli EPEC according to the color of bacterial colonies, and has the advantages of specificity, high efficiency, rapidness, low cost, simple result judgment and the like, and has a wide application prospect in food safety monitoring.

The invention discloses an artificial feed for efficient propagation of low-age larva parasitic wasps of prodenia litura. The artificial feed is prepared from the following raw materials: agar powder, soybean meal, sweet potato vines with leaves, yeast powder, wheat germs, sodium benzoate, vitamin C, multivitamins, linseed oil, chlortetracycline hydrochloride soluble powder and the like. The artificial feed is used for feeding 2-5-year-old prodenia litura larvae parasitized by parasitic wasps, and the pupation rate and eclosion rate of the parasitic wasps can both exceed 93%.

This invention discloses a culture medium for the mass sporulation of *Substantia macrantha* and its application. The culture medium comprises the following components: lactose 21-23 g / L, yeast extract 14-16 g / L, potassium chloride 0.4-0.6 g / L, magnesium sulfate 0.4-0.6 g / L, potassium dihydrogen phosphate 0.4-0.6 g / L, disodium hydrogen phosphate 0.55-0.75 g / L, sodium molybdate dihydrate 5-7 mg / L, vitamin B6 50-60 mg / L, arginine 20-30 mg / L, and agar 18-22 g / L, diluted to volume with double-distilled water, with a natural pH. Using the culture medium prepared according to this invention to culture *Substantia macrantha* significantly increases the spore yield (164-fold), reaching 2.21 × 10⁻⁶. 8 conidia / cm 2 This provides a reference for the mass production of the fungal spores.

The invention discloses a lactobacillus lactis for relieving constipation and a preparation method thereof, target strains are respectively separated from a fermented bean curd sample and screened by a pectinase screening agar culture medium, and are named as lactobacillus plantarum PD-14 and lactobacillus pentosus PD-16, the lactobacillus lactis has a gene for synthesizing pectate lyase, and the lactobacillus lactis has a gene for synthesizing pectate lyase. Relates to the microbial field. The invention provides a beneficial bacterium for relieving constipation and a preparation method thereof, wherein the beneficial bacterium is capable of efficiently relieving constipation, low in cost, stable and easy to industrialize.

The invention relates to the technical field of Escherichia coli detection, and particularly discloses a semisolid culture medium for rapid detection of Escherichia coli and a detection method and application thereof, the semisolid culture medium comprises the following components by weight: 4.2-5.8 g / L of agar; 8.5 to 11.5 g / L of tryptone; the semi-solid culture medium comprises the following components: 3.2 to 4.8 g / L of beef extract, 6.5 to 9.5 g / L of lactose, 1.2 to 2.2 g / L of D-glucose, 4.2 to 5.8 g / L of sodium chloride, 42000 to 58000 [mu] g / L of penicillin, 0.12 to 0.28 g / L of cholate, 0.06 to 0.14 g / L of disodium EDTA (Ethylene Diamine Tetraacetic Acid), 0.12 to 0.22 g / L of L-cysteine, 1.2 to 2.8 g / L of yeast extract, 0.005 to 0.015 g / L of vitamin B and an acid-base indicator, the semisolid culture medium and the detection method thereof provided by the invention not only solve the core pain point of the existing escherichia coli detection method in the technical level, but also reduce the operation cost and improve the benefit for enterprises in the economic level, promote the standardization and high efficiency of the food microorganism detection technology in the industry level, and have practicability and industrial popularization value.

The invention relates to the technical field of fungus culture, and discloses a culture medium for inducing Stemphylium solani to produce spores, a preparation method and application, the preparation raw materials of the culture medium comprise a tomato extract, a carrot extract and agar, the total mass of the tomato extract and the carrot extract is 50g / L to 200g / L, and the content of the tomato extract is not less than 50g / L. According to the culture medium for inducing the Stemphylium solani to produce the spores, the preparation method and the application, the tomato extract and the carrot extract in a specific ratio are combined, so that optimal nutrition and environmental conditions are provided for the Stemphylium solani to produce the spores. Experimental data show that the sporulation quantity of the culture medium is remarkably higher than that of a conventional culture medium (such as CA, V8, PDA and the like), the technical problem that an existing solid culture medium is unstable in sporulation or does not produce sporulation is solved, the selected core raw materials tomato and carrot are common fruits and vegetables, low in cost and easy to obtain, expensive or special chemical reagents are avoided, and the production cost is reduced. And the research and development and application cost is greatly reduced.

The application discloses a tissue culture propagation method of eriochloa contracta. The creeping stem section of the eriochloa contracta is collected, washed, and then sterilized and dried; the sterilized and treated stem section is inoculated into a bud induction medium of MS+1-1.5 mg / L 6-BA+0.1-0.5 mg / L NAA+30 mg / L sucrose+6.5 mg / L agar to perform bud induction, and after 15-20 days of culture, the adventitious bud seedling is obtained; the adventitious bud of about 1-1.5 cm induced in the primary culture medium is inoculated into a proliferation culture medium to perform subculture, and the next step can be divided into single bud expansion or direct transplanting domestication. The two-step method only needs primary culture-proliferation and rooting to be simultaneously performed, and the transplanting survival rate is high.

The application belongs to the technical field of plant disease prevention and treatment, and relates to a determination method for visually and rapidly evaluating the effect of pesticides on inhibition of soil-borne pathogen infection on crop root systems. Agar powder is added to deionized water and boiled until dissolved, sterilized by high-pressure steam and cooled to 60-65 DEG C to obtain an agar culture medium, and the agar culture medium is poured into a culture dish and continuously cooled to solidification. A double-layer filter paper is laid on the solidified culture medium, pre-germinated seeds are placed above the filter paper and cultivated until the root system expands. After the root system of the seedling expands, the pathogen is inoculated and pesticide liquid is applied; and the activity of the pesticide on the pathogen is evaluated. The advantages of the application are that rapid inoculation can be realized, symptoms can be observed in real time, nematodes can be rapidly inoculated and dyed, pesticides can be applied at different stages before, during and after pathogen infection, and the protective and therapeutic activities of the pesticide can be rapidly evaluated. The application greatly facilitates the related research on pathogen or nematode infection, and provides a rapid and efficient method for evaluating the activity of the pesticide on the soil-borne pathogen.

The application discloses a kind of preparation and suspension culture of blackland callus method, belong to the field of industrial biotechnology.It includes the following steps: after sterilization, take flower stem, access pH value is 5-7 in induction medium, under the condition of temperature is 20-30 DEG C and no light, induction culture 15-21 days, namely obtain blackland callus, wherein, the induction medium is made of MS basic medium, sucrose 25-35g / L, agar 6-7g / L, 6-BA 1.0-2mg / L, NAA 0.5-1.5mg / L, 2, 4-D 0.3-1.0mg / L and coconut water 0.5-1.5g / L composition.Under aseptic conditions, clamp the growth state of good blackland embryogenic callus 30g, inoculation is carried out in 200mL / 500mL pH value is 6.0 in MS liquid medium, temperature 25 DEG C, 110 rpm full light oscillation culture, subculture period 7 days, when subculture, the mixture of cell and culture fluid is mixed with fresh culture fluid according to 1:1 volume ratio, after equal division, continue to culture, namely obtain blackland suspension cell, wherein, the liquid medium is made of MS basic medium, sucrose 30g / L, 6-BA 1.5mg / L, NAA 0.5mg / L, 2, 4-D 0.5mg / L and coconut water 1.5g / L composition.The callus out of the rate of 88.6% is realized, and the induction rate of embryogenic callus that can be suspended culture is 68.6%-91.8%, and good callus state is provided for large-scale culture blackland cell.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

This invention relates to a rapid induction culture medium and method for inducing bud formation from kiwifruit leaves to complete plants, belonging to the field of biotechnology. The callus induction culture medium for rapidly inducing bud formation from kiwifruit leaves includes MS medium, sucrose, 6-benzylaminopurine, naphthaleneacetic acid, thidiazuron, and agar, with the following weight ratios: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.000001%-0.000003%, naphthaleneacetic acid 0.0000002%-0.0000008%, thidiazuron 0.000001%-0.000003%, agar 0.006%-0.01%, and the balance being water. The formula for the callus induction bud culture medium is as follows: MS medium 0.002%-0.006%, sucrose 0.01%-0.05%, 6-benzylaminopurine 0.0000005%-0.000003%, naphthaleneacetic acid 0.00000005%-0.0000002%, agar 0.006%-0.01%, with the balance being water. This invention provides a rapid induction culture medium and method for kiwifruit leaves to complete plants, effectively inhibiting problems such as callus waterlogging, browning, and vitrification. It not only increases the number of buds but also improves the quality of buds, which is beneficial to the growth and differentiation of callus tissue, making it easier for it to differentiate into buds, thus laying a solid foundation for subsequent genetic transformation experiments.

The invention discloses a preparation method and application of carassius auratus rahn aquatic OmpA gene cloning and prokaryotic expression as well as a polyclonal antibody. The preparation method comprises the following steps: culture and DNA amplification of rahn aquatic, design of an OmpA primer, gene cloning and prokaryotic expression, back multi-point subcutaneous injection of an immune rabbit, heart blood sampling and preparation of the polyclonal antibody. The invention further provides OmpA protein prokaryotic expression and protein purification, preparation of OmpA polyclonal antiserum and application of fish bodies. According to the invention, a rahnella aquatilis OmpA gene is cloned, a specific Hind III and EcoR1 double-enzyme digestion primer sequence OmpA-1F / OmpA-1R is designed, a prokaryotic expression vector pET32a-OmpA is constructed, high-immunogenicity OmpA protein is obtained through purification, OmpA specific multi-antibody serum is prepared, and the OmpA specific multi-antibody serum can be used for preparing the multi-antibody serum. The OmpA polyclonal antibody can be used for detecting and positioning the expression application of rahnella aquatica OmpA protein in crucian carp bodies through bidirectional agar diffusion, an immunoblotting method and an immunohistochemical method, and provides a reference basis for research and development and application of rahnella aquatica subunit vaccines and a molecular diagnosis technology as well as immune prevention and control of infectious diseases of the rahnella aquatica subunit vaccines and the molecular diagnosis technology.

The preparation method of the agar nanogel interface modification spray is prepared from the following raw materials by weight: agarose 35-70 parts, Tween 80 40-50 parts, dimethyl silicone oil 60-150 parts, and tetrahydrofuran 50-120 parts. The preparation method comprises the following steps: S1: placing agarose into heated deionized water to obtain an agarose aqueous solution, adding Tween 80 and dimethyl silicone oil to obtain a coarse emulsion; S2: ultrasonic dissolving the coarse emulsion to obtain a fine emulsion, cooling and centrifuging, taking the lower liquid, adding tetrahydrofuran to obtain an agar nanogel mixture; S3: rotary evaporation and drying the mixture to obtain agar nanogel; and S4: adding the gel into ethanol or water to obtain the agar nanogel interface modification spray. The agar nanogel spray formed based on the molecular self-assembly strategy has a hexagonal phase columnar structure, the spray can form a modified layer with excellent light-heat conversion efficiency and water transport performance on the water surface, and can significantly enhance the evaporation efficiency.

The invention belongs to the technical field of cosmetics, and discloses a slow-release low-irritation heating composition as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) adding agar, sodium alginate, a humectant, a thickener, a water-soluble preservative and water into an emulsifying pot, heating and stirring until the materials are completely dissolved, then sequentially adding a skin conditioner, vanillyl butyl ether and phenoxyethanol, uniformly stirring, and preserving heat for later use; and (2) dropping the heat-insulating mixed material obtained in the step (1) into cooling oil of 0-5 DEG C through a pill dropping machine, cooling to obtain a spherical object, conveying the spherical object to a cleaning pool, and cleaning oil on the surface of the spherical object to obtain the slow-release low-irritation heating composition. According to the invention, vanillyl butyl ether is adopted as a heating component, and wrapping treatment is carried out through a specific preparation process, so that certain slow release can be achieved, irritation can be reduced, the thermal inductance effect of ginger can be better simulated, and the thermal inductance stability can be improved.