The application discloses a method for disinfecting and inducing regeneration of a single excellent
plant of
Malus baccata, and comprises the following steps: S1, disinfecting the terminal
bud or / and
axillary bud of the
Malus baccata; S2, inoculating the terminal
bud or / and
axillary bud into a modified MS culture medium, and culturing under preset conditions to obtain uncontaminated terminal
bud or / and
axillary bud; S3, inoculating the uncontaminated terminal bud or / and axillary bud into an induction culture medium, and culturing under preset induction conditions to obtain a cluster bud, wherein the components of the induction culture medium comprise a modified MS as a base, and 6-BA, IPA,
prochloraz,
cysteine hydrochloride and L-
arginine are added; and S4, dividing the cluster bud into single plants, inoculating the single plants into a subculture medium, and culturing under preset culture conditions to obtain
Malus baccata single seedlings. Compared with the prior art, the method selects a disinfection method with a
high survival rate and a modified MS culture medium with obvious
plant life characteristics by taking the terminal bud or / and axillary bud of the
Malus baccata as explant material, so that more uncontaminated
Malus baccata terminal buds or / and axillary buds which grow well can be obtained, and the
tissue culture can be carried out for a long time, thereby providing more materials for the
tissue culture of the
Malus baccata.