11 fungicide compositions mainly used for controlling crop diseases caused by pyricularia oryzae and colletotrichum spp.

Abstract

11 fungicide compositions mainly used for controlling crop diseases caused by Pyricularia oryzae and Colletotrichum spp.. Compound YJY-22 has a novel structure and a unique mode of action, and exhibits no cross-resistance with isoprothiolane, fenoxanil, pyraclostrobin, tricyclazole, azoxystrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil, or tebuconazole. Because compound YJY-22 and the described agents have different mechanisms of action and have no cross-resistance, combining compound YJY-22 and the described agents not only can broaden the antifungal spectrum and enhance antifungal efficacy, but also can delay fungicide resistance of plant pathogens, ensure high crop safety, expand the control spectrum, and meet the requirement of pesticide reduction with increased efficiency.

Description

Eleven fungicide compositions for controlling crop diseases mainly caused by *Pyrophyllus oryzae* and *Anthracnose*. Technical Field

[0001] This invention belongs to the field of pesticide technology and relates to 11 fungicide compositions that mainly control crop diseases caused by *Pyrophyllus oryzae* and *Anthracnose*. Background Technology

[0002] Rice blast is a plant disease caused by *Pyricularia oryzae*, widely distributed in rice-growing countries and regions, and is one of the most serious diseases affecting rice production. It is estimated that rice blast causes approximately US$5 billion in losses globally each year. Besides infecting rice, *Pyricularia oryzae* can also infect barley, wheat, and some grassy weeds, thus posing a serious threat to global food security.

[0003] Anthracnose is a significant foliar disease of rubber trees caused by fungi of the genus *Colletotrichum*, primarily *Colletotrichum glocosporioides* and *Colletotrichum glocosporioides*. This disease can affect multiple tissues at different leaf ages and growth stages of rubber trees, causing severe losses to natural rubber production. Therefore, safe and efficient control of diseases caused by *Pyrophyllus oryzae* or *Colletotrichum glocosporioides* is of great importance to ensuring the safety of agricultural products in my country.

[0004] Currently, due to the lack of highly resistant crop varieties, chemical control remains the primary method for controlling rice blast and rubber anthracnose. Commonly used fungicides for controlling rice blast include isoprothiolane, isoprothiolane oxychloride, pyraclostrobin, tricyclazole, and azoxystrobin; commonly used fungicides for controlling rubber anthracnose include mancozeb, captan, difenoconazole, prochloraz, chlorothalonil, and tebuconazole. Because these fungicides have been used frequently and for a long time to prevent rice blast and rubber anthracnose, the pathogens have developed varying degrees of resistance to them. Therefore, developing suitable fungicide combinations is particularly important for crop disease control. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to address the shortcomings of the prior art by providing 11 fungicide compositions that mainly control crop diseases caused by *Pyrophyllus oryzae* and *Anthracnose*. These compositions are highly effective, low in toxicity, environmentally friendly, and beneficial for managing pathogen resistance and expanding the spectrum of fungicide activity. They are also highly effective against crop diseases caused by *Pyrophyllus oryzae* or *Anthracnose*.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows:

[0007] This invention discloses a bactericide composition comprising active ingredient A and active ingredient B;

[0008] in,

[0009] The chemical structural formula of active ingredient A is shown below:

[0010] The active ingredient B is selected from any one or a combination of several of the following: isoprothiolane, isoprothiolane, pyraclostrobin, tricyclazole, azoxystrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil, and tebuconazole.

[0011] In some embodiments, preferably, the mass ratio of active ingredient A to active ingredient B is 50:1 to 1:50, more preferably 16:1 to 1:16, and even more preferably 8:1 to 1:8.

[0012] In some embodiments, preferably, when the active ingredient B is selected from isoprothiolane, the mass ratio of the active ingredient A to isoprothiolane is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, even more preferably 8:1 to 2:2 or 1:2, and most preferably 2 to 8:1 or 1:2.

[0013] In some embodiments, preferably, when the active ingredient B is selected from isoprothiolane, the mass ratio of the active ingredient A to the isoprothiolane is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 8:1 to 1:1.

[0014] In some embodiments, preferably, when the active ingredient B is selected from pyraclostrobin, the mass ratio of the active ingredient A to the pyraclostrobin is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 8:1 or 1:1.

[0015] In some embodiments, preferably, when the active ingredient B is selected from tricyclazole, the mass ratio of the active ingredient A to the tricyclazole is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 2:1 to 1:4.

[0016] In some embodiments, preferably, when the active ingredient B is selected from azoxystrobin, the mass ratio of the active ingredient A to the azoxystrobin is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 4:1 to 1:1.

[0017] In some embodiments, preferably, when the active ingredient B is selected from mancozeb, the mass ratio of the active ingredient A to the mancozeb is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 8:1 or 1:1 to 1:2.

[0018] In some embodiments, preferably, when the active ingredient B is selected from captan, the mass ratio of the active ingredient A to the captan is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 1:1 to 1:2.

[0019] In some embodiments, preferably, when the active ingredient B is selected from difenoconazole, the mass ratio of the active ingredient A to the difenoconazole is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 2:1.

[0020] In some embodiments, preferably, when the active ingredient B is selected from imazalil, the mass ratio of the active ingredient A to the imazalil is 50:1 to 1:50, more preferably 16:1 to 1:16, further preferably 8:1 to 1:8, and even more preferably 1:1 to 1:4.

[0021] In some embodiments, preferably, when the active ingredient B is selected from chlorothalonil, the mass ratio of the active ingredient A to the chlorothalonil is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 8:1 to 2:1 or 1:2 to 1:8.

[0022] In some embodiments, preferably, when the active ingredient B is selected from tebuconazole, the mass ratio of the active ingredient A to the tebuconazole is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 1:4.

[0023] In some embodiments, preferably, the total weight of the bactericide composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 11% to 89% of the total weight of the bactericide composition.

[0024] Furthermore, the bactericide composition further includes necessary carriers and / or excipients; the excipients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

[0025] The application of the above-mentioned fungicide composition in the prevention and control of pathogens and the agricultural diseases they cause is also within the scope of protection of this invention.

[0026] In some embodiments, preferably, the pathogens include, but are not limited to, *Pyrrosia lingua* and / or *Anthrax*.

[0027] In some embodiments, and more preferably, the pathogens include, but are not limited to, *Pyrrosia lingua* and / or *Colletotrichum gloeosporioides*.

[0028] Furthermore, the present invention provides a method for preventing and controlling pathogens and the agricultural diseases they cause, comprising applying the above-mentioned fungicide composition to plants with diseases.

[0029] In some embodiments, preferably, the diseases include, but are not limited to, agricultural diseases caused by *Pyrophyllus oryzae* and / or *Anthracnose*.

[0030] In some embodiments, and more preferably, the diseases include, but are not limited to, agricultural diseases caused by *Pyrophyllus oryzae* and / or *Colletotrichum gloeosporioides*.

[0031] In the experiments of this invention, the *Pyricularia oryzae* species include, but are not limited to, the *Pyricularia oryzae* Cav. (brand: Bio-25250, catalog number: Bio-25250) provided in the examples, and the *Anthrax* species include, but are not limited to, the *Colletotrichum gloeosporioides* Penz. (brand: Bio-14325, catalog number: bio-14325, source: Beijing Bio-2525 Biotechnology Co., Ltd.) provided in the examples. Other physiological races of *Pyricularia oryzae* / other physiological races of *Anthrax* species that can be used for virulence testing in the prior art can also be applied to this invention. Beneficial effects:

[0032] (1) The active component of the fungicide composition provided by the present invention is composed of compound YJY-22 and isoprothiolane, isoprothiolane, pyraclostrobin, tricyclazole, pyraclostrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil or tebuconazole. The combination of the two has a synergistic effect, which significantly improves the fungicide activity against rice blast fungus and anthracnose fungus, and has a significant effect on preventing crop diseases caused by rice blast fungus and anthracnose fungus.

[0033] (2) The active components in the fungicide composition provided by the present invention have different mechanisms of action and no cross-resistance, which is beneficial to delay the drug resistance of pathogens, has good safety for crops, expands the control spectrum, and meets the requirements of pesticide reduction and efficiency enhancement.

[0034] (3) The compound YJY-22 provided by the present invention is a cyanoacrylate bactericidal compound that acts on type I myosin (motor protein) of Pyrrosia lingua and Bacillus anthracis, causing the bacteria to lose their growth power and leading to the death of the bacteria.

[0035] (4) The compound YJY-22 provided by this invention has a novel structure and a unique mode of action, and exhibits no cross-resistance with isoprothiolane, isoprothiolane, pyraclostrobin, tricyclazole, azoxystrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil, and tebuconazole. Because compound YJY-22 has a different mechanism of action than the aforementioned agents and exhibits no cross-resistance, this invention combines the two, which not only expands the fungicidal range and enhances the fungicidal effect, but also delays the development of resistance in plant pathogens, provides good safety for crops, broadens the control spectrum, and meets the needs of pesticide reduction and efficiency enhancement. Attached Figure Description

[0036] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments, and the advantages of the present invention in the above and / or other aspects will become clearer.

[0037] Figure 1 shows the EC50 of compound YJY-22 and isoprothiolane in combination with pyrimethanil for the pathogen *Pyrethrum oryzae*. 50 Determine the colony map.

[0038] Figure 2 shows the EC50 of compound YJY-22 and pyroxenamide combined with *Pyroxella oryzae*, the causal agent of rice blast. 50 Determine the colony map.

[0039] Figure 3 shows the EC50 of compound YJY-22 and pyraclostrobin in combination with *Pyracantha fortuneana* (originating pathogen). 50 Determine the colony map.

[0040] Figure 4 shows the EC50 of compound YJY-22 and captan combined with *Colletotrichum gloeosporioides*. 50 Determine the colony map.

[0041] Figure 5 shows the EC50 of compound YJY-22 and difenoconazole combined with *Colletotrichum gloeosporioides*. 50 Determine the colony map.

[0042] Figure 6 shows the EC of compound YJY-22 and imazalil in *Colletotrichum gloeosporioides*. 50 Determine the colony map.

[0043] Figure 7 shows the EC50 of compound YJY-22 and chlorothalonil combined with *Colletotrichum gloeosporioides*. 50 Determine the colony map.

[0044] Figure 8 shows the EC of compound YJY-22 and tebuconazole in *Colletotrichum gloeosporioides*. 50 Determine the colony map. Detailed Implementation

[0045] The present invention will be further described below with reference to specific embodiments. These embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Any modifications or substitutions made to the methods, steps, or conditions of the present invention without departing from the spirit and essence of the invention are within the scope of the invention.

[0046] Unless otherwise specified, the experimental methods used in the following examples are conventional methods; the materials and reagents used are commercially available unless otherwise specified.

[0047] All compound structures in this invention were drawn using KingDraw software.

[0048] Example 1: Preparation of compound YJY-22

[0049] Weigh 5 mmol (0.595 g) of p-hydroxybenzonitrile into a 50 mL round-bottom flask, add 2 mL of 1,2-dichloroethane as solvent, and place in a 2 °C low-temperature reaction bath. Add 50 mmol (4.130 g) of anhydrous hydrogen chloride ethanol solution and stir at low temperature for 40 h for alcoholysis. After the reaction is complete, concentrate by vacuum distillation, allow to stand at low temperature and crystallize to obtain ethyl 4-hydroxybenzyl imine hydrochloride intermediate. Use it directly in the next reaction without purification.

[0050] Weigh 3 mmol (0.605 g) of ethyl 4-hydroxybenzyl imide hydrochloride intermediate into a round-bottom flask, dissolve it in 5 mL of anhydrous ethanol, and then place it in a low-temperature reaction bath at 5–10 °C. Using triethylamine as an acid-binding agent, slowly add (Et3N, 3.6 mmol, 0.367 g) dropwise, and continue stirring for 30 min. Then, slowly raise the temperature to reflux (80 °C), and slowly add ethyl cyanoacetate (3 mmol, 0.339 g) dropwise. After the addition is complete, reflux until the condensation reaction is complete. Monitor the reaction by TLC. After the reaction is complete, remove the solvent by vacuum distillation, and perform column chromatography (DCM:MeOH = 60:1) using dichloromethane and methanol as eluents to give a 2-cyanoacrylate compound, designated as compound YJY-22, a white solid in 78% yield.

[0051] The 1H NMR spectrum data of the 2-cyanoacrylate compound YJY-22 are as follows:

[0052] 1H NMR (300MHz, DMSO-d6) δ10.10(s,1H),9.18(s,1H),8.73(s,1H),7.48 -7.39(m,2H),6.92-6.82(m,2H),4.17(q,J=7.1Hz,2H),1.30 -1.19(m,3H).

[0053] Example 2: Indoor toxicity assay of compound YJY-22 in combination with existing drugs against *Pyrrosia lingua*

[0054] Test subject: Pyricularia oryzae Cav. (brand: Bio-25250).

[0055] Test reagents: Compound YJY-22 (prepared in Example 1, purity 98%), isoprothiolane (purity 98%), isoprothiolane (purity 95.3%), pyraclostrobin (purity 98%), tricyclazole (purity 97%), and azoxystrobin (purity 96%) were prepared and provided by the Fungicide Biology Laboratory of Nanjing Agricultural University.

[0056] Experimental Method: The mycelial growth rate method was referenced in the "Agricultural Industry Standard of the People's Republic of China NY / T 1156.2-2006". Five dosage treatments were set up for each agent based on the effective ingredient content. The *Pyrrosia lingua* species were inoculated onto PDA medium. When the colonies covered 2 / 3 of the culture dish, a 5mm diameter punch was used to break the colonies into small blocks at the edge. These blocks were then transferred to the center of a pre-prepared drug-containing PDA medium using an inoculation needle. The dishes were then incubated at 28℃ for 3 days. The colony diameter (cm) of each treatment was measured using calipers using the cross-crossing method to determine the corrected inhibition percentage. The EC50 of each agent was then calculated using linear regression analysis between the probability value of the inhibition rate and the logarithm of the series concentrations. 50 The value was repeated 4 times per treatment. YJY-22 was set as the standard reagent, and the co-toxicity coefficient (CTC) was calculated according to the following formula: Actual toxicity index (ATI) = (EC value of the standard reagent) / (EC value of the standard reagent) 50 EC of mixed drugs 50 Theoretical toxicity index (TTI) = Standard reagent toxicity index × Percentage of standard reagent in the mixture + Test reagent toxicity index × Percentage of test reagent in the mixture; Co-toxicity coefficient (CTC) = (ATI / TTI) × 100%;

[0057] A CTC value less than 80 indicates that the combination of drugs has an antagonistic effect; a value between 80 and 120 indicates an additive effect; and a value greater than 120 indicates a synergistic effect.

[0058] The test results are shown in Tables 1 to 5. In the tables, A represents compound YJY-22, and B represents isoprothiolane, isoprothiolane amide, pyraclostrobin, tricyclazole, or pyraclostrobin. The ratio in parentheses after A+B in the tables is the mass ratio of A to B.

[0059] Table 1: Results of indoor toxicity assays of YJY-22 combined with isoprothiolane against Pyrethrum indicum.

[0060] Table 1 shows that the rational combination of compound YJY-22 and pyraclostrobin has an additive or synergistic effect on the inhibition of mycelial growth of *Pyralida oryzae*, without any antagonistic effect. The specific mycelial growth inhibition diagram is shown in Figure 1, where the vertical axis represents the mass ratio of compound YJY-22 to pyraclostrobin (8:1, 4:1, 2:1, 1:1, 1:2, 1:4, 1:8, respectively), and the horizontal axis represents the total mass concentration of compound YJY-22 and pyraclostrobin in the drug-containing PDA medium (0.5 μg / mL, 1.0 μg / mL, 2.0 μg / mL, 4.0 μg / mL, 8.0 μg / mL, respectively).

[0061] Table 2: Results of indoor toxicity assays of YJY-22 combined with isoprothiolane against *Pyralidae*.

[0062] Table 2 shows that the appropriate combination of compound YJY-22 and pyroxenamide has an additive or synergistic effect on the inhibition of mycelial growth of *Pyroxburghii*, without any antagonistic effect. The specific mycelial growth inhibition diagram is shown in Figure 2. The vertical axis represents the mass ratio of compound YJY-22 to pyroxenamide (8:1, 4:1, 2:1, 1:1, 1:2, respectively), and the horizontal axis represents the total mass concentration of compound YJY-22 and pyroxenamide in the drug-containing PDA medium (0.25 μg / mL, 0.5 μg / mL, 1.0 μg / mL, 2.0 μg / mL, 4.0 μg / mL, respectively).

[0063] Table 3: Results of indoor toxicity assays of YJY-22 combined with pyraclostrobin against *Pyracanthae* sp.

[0064] Table 3 shows that the optimal combination of compound YJY-22 and pyraclostrobin has an additive or synergistic effect on inhibiting the mycelial growth of *Pyracantha fortuneana*, without any antagonistic effect. The specific mycelial growth inhibition diagram is shown in Figure 3. The vertical axis represents the mass ratio of compound YJY-22 to pyraclostrobin (8:1, 4:1, 2:1, 1:1, 1:2, 1:4, 1:8, respectively), and the horizontal axis represents the total mass concentration of compound YJY-22 and pyraclostrobin in the drug-containing PDA medium (0.002 μg / mL, 0.01 μg / mL, 0.04 μg / mL, 0.16 μg / mL, 0.64 μg / mL, respectively).

[0065] Table 4: Results of indoor toxicity assays of YJY-22 combined with tricyclazole against *Pyrrosia lingua*.

[0066] The results in Table 4 show that the rational combination of compound YJY-22 and tricyclazole has a synergistic or additive effect on the inhibition of mycelial growth of *Pyrrosia lingua*, without any antagonistic effect.

[0067] Table 5: Results of indoor toxicity assays of YJY-22 combined with azoxystrobin against *Pyracanthae* sp.

[0068] The results in Table 5 show that the rational combination of compound YJY-22 and azoxystrobin has a synergistic or additive effect on the inhibition of mycelial growth of *Pyrrosia lingua*, without any antagonistic effect.

[0069] Example 3: Indoor toxicity determination of YJY-22 in combination with existing drugs against Colloidal anthrax.

[0070] Test subject: Colletotrichum gloeosporioides Penz. (Brand: Bio-Bio, Product No.: bio-14325, Source: Beijing Bio-Bio Biotechnology Co., Ltd.)

[0071] Test agents: YJY-22 (prepared in Example 1, purity 98%), mancozeb (purity 80%), captan (purity 95%), difenoconazole (purity 96.3%), prochloraz (purity 98%), chlorothalonil (purity 96%), and tebuconazole (purity 97%) were prepared and provided by the Fungicide Biology Laboratory of Nanjing Agricultural University.

[0072] Experimental Method: The mycelial growth rate method was referenced in the "Agricultural Industry Standard of the People's Republic of China NY / T 1156.2-2006". Five doses of each agent were prepared based on the effective ingredient content. The *Colletotrichum gloeosporioides* was inoculated onto PDA medium. When the colonies covered 2 / 3 of the culture dish, a 5mm diameter punch was used to break the colonies into small blocks at the edge. These blocks were then transferred to the center of a pre-prepared drug-containing PDA medium using an inoculation needle. The plates were then incubated at 25℃ for 3 days. The colony diameter (cm) of each treatment was measured using calipers using the cross-sectional method. The corrected inhibition percentage was calculated. Then, linear regression analysis between the probability value of the inhibition rate and the logarithm of the series concentrations was used to calculate the EC50 of each agent. 50 The value was repeated 4 times per treatment. YJY-22 was set as the standard reagent, and the co-toxicity coefficient (CTC) was calculated according to the following formula: Actual toxicity index (ATI) = (EC value of the standard reagent) / (EC value of the standard reagent) 50 EC of mixed drugs 50Theoretical toxicity index (TTI) = Standard reagent toxicity index × Percentage of standard reagent in the mixture + Test reagent toxicity index × Percentage of test reagent in the mixture; Co-toxicity coefficient (CTC) = (ATI / TTI) × 100%;

[0073] A CTC value less than 80 indicates that the combination of drugs has an antagonistic effect; a value between 80 and 120 indicates an additive effect; and a value greater than 120 indicates a synergistic effect.

[0074] The test results are shown in Tables 6 to 11. In the tables, A represents compound YJY-22, and B represents mancozeb, captan, difenoconazole, imazalil, chlorothalonil, or tebuconazole. The ratio in parentheses after A+B in the tables is the mass ratio of A to B.

[0075] Table 6: Results of indoor toxicity assays of YJY-22 and mancozeb combined with *Colletotrichum gloeosporioides*

[0076] The results in Table 6 show that the rational combination of compound YJY-22 and mancozeb has an additive or synergistic effect on inhibiting the mycelial growth of Colloidal anthracnose, without any antagonistic effect.

[0077] Table 7: Results of Indoor Toxicity Tests of YJY-22 and Captan Combined with Colchicine for Anthracnose

[0078] Table 7 shows that the appropriate combination of compound YJY-22 and captan has an additive or synergistic effect on the inhibition of mycelial growth of *Colletotrichum gloeosporioides*, without any antagonistic effect. The specific mycelial growth inhibition diagram is shown in Figure 4. The vertical axis represents the mass ratio of compound YJY-22 to captan (8:1, 4:1, 2:1, 1:1, 1:2, 1:4, 1:8, respectively), and the horizontal axis represents the total mass concentration of compound YJY-22 and captan in the drug-containing PDA medium (0.25 μg / mL, 0.5 μg / mL, 1.0 μg / mL, 2.0 μg / mL, 4.0 μg / mL, 8.0 μg / mL, respectively).

[0079] Table 8: Results of indoor toxicity assays of YJY-22 combined with difenoconazole against *Colletotrichum gloeosporioides*

[0080] Table 8 shows that the optimal combination of compound YJY-22 and difenoconazole has an additive or synergistic effect on inhibiting the mycelial growth of *Colletotrichum gloeosporioides*, without any antagonistic effect. The specific mycelial growth inhibition diagram is shown in Figure 5. The vertical axis represents the mass ratio of compound YJY-22 to difenoconazole (8:1, 4:1, 2:1, 1:1, 1:2, 1:4, 1:8, respectively), and the horizontal axis represents the total mass concentration of compound YJY-22 and difenoconazole in the drug-containing PDA medium (0.125 μg / mL, 0.25 μg / mL, 0.5 μg / mL, 1.0 μg / mL, 2.0 μg / mL, 4.0 μg / mL, respectively).

[0081] Table 9: Results of indoor toxicity assays of YJY-22 combined with imazalil against *Colletotrichum gloeosporioides*.

[0082] Table 9 shows that the appropriate combination of compound YJY-22 and prochloraz has an additive or synergistic effect on the inhibition of mycelial growth of *Colletotrichum gloeosporioides*, without any antagonistic effect. The specific mycelial growth inhibition diagram is shown in Figure 6. The vertical axis represents the mass ratio of compound YJY-22 to prochloraz (8:1, 4:1, 2:1, 1:1, 1:2, 1:4, 1:8, respectively), and the horizontal axis represents the total mass concentration of compound YJY-22 and prochloraz in the drug-containing PDA medium (0.015625 μg / mL, 0.03125 μg / mL, 0.0625 μg / mL, 0.125 μg / mL, 0.25 μg / mL, 0.5 μg / mL, respectively).

[0083] Table 10: Indoor toxicity test results of YJY-22 combined with chlorothalonil against *Colletotrichum gloeosporioides*

[0084] Table 10 shows that the appropriate combination of compound YJY-22 and chlorothalonil has an additive or synergistic effect on the inhibition of mycelial growth of *Colletotrichum gloeosporioides*, without any antagonistic effect. The specific mycelial growth inhibition diagram is shown in Figure 7. The vertical axis represents the mass ratio of compound YJY-22 to chlorothalonil (8:1, 4:1, 2:1, 1:1, 1:2, 1:4, 1:8, respectively), and the horizontal axis represents the total mass concentration of compound YJY-22 and chlorothalonil in the drug-containing PDA medium (0.25 μg / mL, 0.5 μg / mL, 1.0 μg / mL, 2.0 μg / mL, 4.0 μg / mL, 8.0 μg / mL, respectively).

[0085] Table 11: Results of indoor toxicity assays of YJY-22 combined with tebuconazole against *Colletotrichum gloeosporioides*.

[0086] Table 11 shows that the rational combination of compound YJY-22 and tebuconazole has an additive or synergistic effect on the inhibition of mycelial growth of *Colletotrichum gloeosporioides*, without any antagonistic effect. The specific mycelial growth inhibition diagram is shown in Figure 8. The vertical axis represents the mass ratio of compound YJY-22 to tebuconazole (8:1, 4:1, 2:1, 1:1, 1:4, respectively), and the horizontal axis represents the total mass concentration of compound YJY-22 and tebuconazole in the drug-containing PDA medium (0.25 μg / mL, 0.5 μg / mL, 1.0 μg / mL, 2.0 μg / mL, 4.0 μg / mL, respectively).

[0087] In summary, the indoor bioassay results show that the composition provided by this invention has a significant synergistic effect, and the composition consists of active ingredients with different mechanisms of action, which is beneficial for overcoming and delaying the development of pathogen resistance. The composition provided by this invention has excellent control efficacy against crop diseases caused by *Pyrophyllus oryzae* and *Anthracnose*.

[0088] This invention provides ideas and methods for 11 fungicide compositions for controlling crop diseases mainly caused by *Pyrophyllus oryzae* and *Anthracnose*. Many methods and approaches exist for implementing this technical solution; the above description is merely a preferred embodiment of the invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this invention, and these improvements and modifications should also be considered within the scope of protection of this invention. All components not explicitly stated in this embodiment can be implemented using existing technologies.

Claims

1. A bactericide composition, characterized in that, The bactericide composition comprises active ingredient A and active ingredient B; in, The chemical structural formula of active ingredient A is shown below: The active ingredient B is selected from any one or a combination of several of the following: isoprothiolane, isoprothiolane, pyraclostrobin, tricyclazole, azoxystrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil, and tebuconazole.

2. The bactericide composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 50:1 to 1:50, preferably 16:1 to 1:16, and more preferably 8:1 to 1:

8.

3. The bactericide composition according to claim 1, characterized in that, When the active ingredient B is selected from isoprothiolane, the mass ratio of active ingredient A to isoprothiolane is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, even more preferably 8:1 to 2:2 or 1:2, and most preferably 2 to 8:1 or 1:2; or, When the active ingredient B is selected from isoprothiolane, the mass ratio of active ingredient A to isoprothiolane is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 8:1 to 1:1; or, When the active ingredient B is selected from pyraclostrobin, the mass ratio of the active ingredient A to the pyraclostrobin is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 8:1 or 1:1; or, When the active ingredient B is selected from tricyclazole, the mass ratio of the active ingredient A to the tricyclazole is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 2:1 to 1:4; or, When the active ingredient B is selected from azoxystrobin, the mass ratio of the active ingredient A to the azoxystrobin is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 4:1 to 1:1; or, When the active ingredient B is selected from mancozeb, the mass ratio of the active ingredient A to the mancozeb is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 8:1 or 1:1 to 1:2; or, When the active ingredient B is selected from captan, the mass ratio of the active ingredient A to the captan is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 1:1 to 1:2; or, When the active ingredient B is selected from difenoconazole, the mass ratio of the active ingredient A to the difenoconazole is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 2:1; or, When the active ingredient B is selected from imazalil, the mass ratio of the active ingredient A to the imazalil is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 1:1 to 1:4; or, When the active ingredient B is selected from chlorothalonil, the mass ratio of the active ingredient A to the chlorothalonil is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 8:1 to 2:1 or 1:2 to 1:8; or, When the active ingredient B is selected from tebuconazole, the mass ratio of the active ingredient A to the tebuconazole is 50:1 to 1:50, preferably 16:1 to 1:16, more preferably 8:1 to 1:8, and even more preferably 1:

4.

4. The bactericide composition according to claim 1, characterized in that, The total weight of the bactericide composition is 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 11% to 89% of the total weight of the bactericide composition.

5. The use of the fungicide composition according to any one of claims 1 to 4 in the prevention and control of pathogens and the agricultural diseases they cause.

6. The application according to claim 5, characterized in that, The pathogens mentioned are *Pyrrosia lingua* and / or *Anthracis*.

7. A method for preventing and controlling pathogens and the agricultural diseases they cause, characterized in that, This includes applying the fungicide composition according to any one of claims 1 to 4 to plants with disease.

8. The method according to claim 7, characterized in that, The diseases mentioned are agricultural diseases caused by *Pyrophyllus oryzae* and / or *Anthracnose*.

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