16526results about "Fungi" patented technology

The invention relates to ice wine yeast with high fermentation activity and outstanding aroma-producing characteristics and application thereof. The ice wine yeast is yeast (Saccharomyces cerevisiae) YSL16010, and the bacterial strain has been preserved in China General Microbiological Culture Collection Center on April 18, 2016, with a preservation number of CGMCC No.12349. The bacterial strain YSL16010 is obtained by separating and screening Vidal grapes from a Huanren producing area of Liaoning at a temperature of 16 DEG C in a natural fermentation process, and the ice wine wine-making yeast having good making characteristics and highly producing esters is obtained through a series of screening and identifications and small pilot scale fermentation. The ice wine wine-making yeast YSL1610 highly producing esters is used for fermenting and producing ice wine, so that the yield of volatile acid is effectively controlled while the standards of the grape wine producing countries can be met, and therefore, the content of aromatic compounds such as esters is remarkably increased. The bacterial strain can be applied to ice wine brewing industry, and can play an important role in improving the quality of the ice wine and bringing advantages of the grape producing areas into play.

To provide VHH-containing polypeptides that bind CD123, and a method for treating cancer that includes administration of the VHH-containing polypeptides.SOLUTION: Provided is a polypeptide comprising at least one VHH domain that binds to CD123, comprising CDR1, CDR2, and CDR3, each comprising a specific amino acid sequence.SELECTED DRAWING: Figure 3A

The invention provides a genetically engineered bacterium, a preparation method thereof and application of the genetically engineered bacterium in synthesis of sclareol. The genetically engineered bacterium comprises a CcLPPS gene derived from Cistanus crenatus, and a CcLPPS gene derived from Cistanus crenatus, the genetically engineered bacterium is selected from yarrowia lipolytica. According to the invention, the stably expressed sclareol efficient de novo synthesis strain is constructed, and no organic reagent or any path intermediate substance is added in the whole fermentation process, so that the production cost is greatly reduced, and the subsequent separation and purification steps are simplified. The engineering yarrowia lipolytica strain provided by the invention is high in production intensity and simple in fermentation process, has a relatively good application prospect, and lays a foundation for producing sclareol by a microbial fermentation method.

To provide antibodies that bind to lymphocyte activation gene 3 (LAG3) protein, which is a T cell co-inhibitor, and methods of use.SOLUTION: In various embodiments of the invention, the antibodies are fully human antibodies that specifically bind to LAG3. In some embodiments, the antibodies of the invention are useful for inhibiting or neutralizing LAG3 activity, thus providing means of treating a disease or disorder such as cancer or viral infection. The antibodies of the present invention are useful, inter alia, for targeting immune cells expressing LAG3 and for modulating LAG3 activity.SELECTED DRAWING: None

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention discloses a Pichia kluyveri yeast and its application. The Pichia kluyveri yeast is classified and named as Pichia kluyveri JLG30201, which has been deposited in the China Center for Type Culture Collection, with a deposit number of CCTCC NO: M 20242295 and a deposit date of October 22, 2024. The Pichia kluyveri JLG30201 has strong killer affinity and high sugar tolerance, unique aroma production, high production of extracellular superoxide dismutase (SOD), and low alcohol production; the strain is applied to the processing of fruit wine or edible plant enzymes, and has the effects of increasing ester and flavor, improving juice yield and product antioxidant activity.

The invention provides PDRN as well as a preparation method and application thereof, and relates to the technical field of biology. And the GC content of the PDRN is 40.5%-42%. According to the present invention, the similarity between the GC% in the gDNA in the PDRN prepared by using the recorded method and the GC% of the human body is more than 98%, the GC% is closer to the GC% of the human body than the PDRN derived from the salmon, the safety and the efficiency are high, and the PDRN preparation method is simple and convenient compared with the PDRN extracted from the salmon semen in the prior art.

The invention provides a construction method and application of a pichia pastoris strain capable of efficiently secreting and expressing recombinant human-derived protein, and relates to the technical field of synthetic biology. According to the invention, pichia pastoris is used as a chassis strain, a ku70 gene and / or a pep4 gene in a pichia pastoris genome are / is knocked out through a gene editing technology, and a new signal peptide O factor signal is introduced, so that the secretion efficiency of the pichia pastoris for secreting and expressing recombinant human-derived protein can be remarkably improved, the problem of protein degradation is effectively improved, and the recombinant human-derived protein can be obtained. And meanwhile, a high-copy pichia pastoris strain is efficiently obtained, so that the expression quantity of recombinant human protein is improved, and powerful support is provided for research and development of related drugs and biological manufacturing.

The invention relates to a recombinant A type humanized XVII type collagen as well as construction and application thereof, the protein is the humanized XVII type collagen which is composed of an intracellular region, a transmembrane region and an extracellular region, and the amino acid sequence is as shown in SEQ No. 1. The hair follicle repairing agent has the advantages of hair follicle repairing, hair regeneration, good biological activities of cell proliferation, cell adhesion and the like, and can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like. The gene sequence of the recombinant A type XVII collagen is 100% identical to the corresponding part of the gene sequence of the recombinant A type XVII collagen, so that the recombinant A type XVII collagen has good hydrophilicity and high activity, and has application potential in the fields of biomedical materials, beauty cosmetics, skin care products and the like.

The invention provides a technical method for improving the anti-aging effect of eurotium cristatum tea compound fermentation liquor, and belongs to the technical field of microorganisms, the Latin name of eurotium cristatum is Eurotium cristatum, the eurotium cristatum is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 2024643. According to the method, the yeast seed solution is inoculated into the tea-sea buckthorn leaf culture solution fermented by the eurotium cristatum, so that the content of anti-aging active components such as procyanidine B2, quercitrin, cyanidin-3-glucoside, nicotinic acid, naringenin and rutin in the fermentation culture solution can be remarkably increased through co-fermentation, and the elastase inhibition rate can be remarkably increased; the anti-aging effect of a component system of the fermentation culture solution is enhanced. The prepared tea-hippophae rhamnoides leaf compound fermentation liquor is suitable for development of anti-aging cosmetics and has a wide market prospect.

The invention relates to the technical field of biology, and discloses yarrowia lipolytica engineering bacteria with high yield of p-coumaric acid by taking glucose as a substrate as well as a construction method and an application of the yarrowia lipolytica engineering bacteria. The construction method comprises the following steps: integrating a tyrosine ammonolysis enzyme gene TAL into a Yarrowia lipolytica strain by adopting a CRISPR / Cas9 positioning integration method, strengthening DAHP synthetase genes ARO4, DHS1 and AROG, overexpressing a tyrosine synthetase TYR gene and a histidine phosphate aminotransferase HIS5 protease gene, and carrying out high-throughput screening to obtain the Yarrowia lipolytica strain. And further integrating exogenous phenylalanine deamination hydroxylation pathway genes for synthesizing p-coumaric acid, wherein the exogenous phenylalanine deamination hydroxylation pathway genes comprise a phenylalanine ammonialyase gene, a cinnamate hydroxylase gene and a P450 reductase gene. The yield of p-coumaric acid produced by the Yarrowia lipolytica engineering strain is the highest yield of known Yarrowia lipolytica, the highest yield in a shake flask can reach 1.7 g / L, the fed-batch fermentation yield in a 5L fermentation tank can reach 30 g / L, and the Yarrowia lipolytica engineering strain has remarkable industrial application value.

The invention provides a high-stability recombinant XVII type collagen as well as a construction method and application thereof, and belongs to the technical field of bioengineering. According to the invention, an amino acid sequence of natural human (XVII) type collagen is selected as a basic unit and repeated for multiple times to obtain recombinant (XVII) type collagen; in the design process of the recombinant XVII type collagen, an unstable GXY triad is avoided, potential matrix metalloproteinase restriction enzyme cutting sites and glycosylation sites are abandoned, a KGD active sequence is reserved, and the probability of containing sites which are easy to degrade can be reduced; a sequence fragment of the recombinant XVII type collagen is derived from a full-length sequence of a natural collagen gene, has excellent solubility, biological activity and high stability, and can be kept stable in a high-temperature storage environment and under the condition of avoiding alkalinity; the product can be used as a raw material in the production process of medicines, medical equipment, biological materials or cosmetics, and has good practicability.

The invention discloses aureobasidium pullulans, application thereof and a method for producing medium-molecular-weight pullulan, and belongs to the technical field of microorganisms. The aureobasidium pullulans disclosed by the invention is aureobasidium pullulans SYCY-018, the strain is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 26, 2022, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.40467. According to the aureobasidium pullulans SYCY-018, the aureobasidium pullulans SYCY-018 and the aureobasidium pullulans The aureobasidium pullulans SYCY-018 is inoculated into a fermentation medium for fermentation to produce the pullulan, the yield of the medium-molecular-weight pullulan reaches up to 150 g / L or above, meanwhile, good hereditary stability and high temperature resistance are achieved, the yield of the pullulan can still be higher than 130 g / L even at the high temperature of 40 DEG C, and the aureobasidium pullulans SYCY-018 is suitable for industrial production.

The present invention relates to a recombinant yeast with high squalene production, a construction method and a use thereof, wherein the recombinant yeast is a saccharomyces cerevisiae that expresses or overexpresses HMGR gene, ADA gene, LEU2 gene, MVA pathway gene and squalene synthase gene ERG9. The present invention integrates acetaldehyde dehydrogenase gene ADA and HMG-CoA reductase gene tHMGR in multiple copies in saccharomyces cerevisiae, integrates the screened high-activity phosphoketolase gene PK and phosphotransacetylase gene PTA, weakens epoxysqualene synthesis, strengthens the mevalonate pathway, overexpresses yeast endogenous squalene synthase gene, etc. to obtain a recombinant yeast with high squalene production, so that the production in the shake flask stage of the production process exceeds 5g / L, and the production in the fed batch fermentation stage exceeds 50g / L, breaking through the current bottleneck of microbial synthesis of squalene production, and is suitable for practical promotion and application.

The invention relates to a saccharomyces cerevisiae strain for efficiently synthesizing cordycepin as well as a construction method and application of the saccharomyces cerevisiae strain, and belongs to the technical field of microbial genetic engineering. The construction method provided by the invention comprises the following steps: carrying out multi-copy genome integrated expression on heterologous codon optimized 2 '-carbonyl-3'-deoxyadenosine reductase genes and 3 '-adenosine monophosphate phosphohydrolase genes, so as to increase the dosage of key enzymes; and supplementing the defective gene of the engineering strain to obtain the saccharomyces cerevisiae strain B5U3 with high yield of cordycepin. According to the obtained saccharomyces cerevisiae strain for efficiently synthesizing the cordycepin, the cordycepin yield reaches 2.92 g / L after shake flask fermentation, the cordycepin yield reaches 7.5 g / L after fermentation in a fermentation tank, and a foundation is laid for safer and more reliable industrial production of the cordycepin.

The invention provides recombinant VII type collagen as well as a preparation method and application thereof, and relates to the technical field of bioengineering. According to the invention, the human-derived VII type collagen is designed and screened, so that a new functional repetitive unit with biological activity of the VII type humanized collagen is successfully obtained, and a new direction is provided for research of the VII type collagen. In the application, three new high-yield recombinant VII type humanized collagens are successfully obtained by repeating the functional repeating units. The recombinant VII type humanized collagen has the effect of improving cell viability, and the protein is proved to be high in safety. The protein also can promote cell adhesion, so that the protein has an excellent effect of treating skin injury. The protein can also improve the cell migration rate and has excellent transdermal absorption performance. In addition, the protein also has the effect of promoting collagen expression, and provides a new functional component for anti-aging related products.

The invention discloses thermophilic fungus cleavable polysaccharide monooxygenase and application thereof, and belongs to the technical field of enzyme engineering and microbial engineering. The thermophilic fungus lysing polysaccharide monooxygenase provided by the invention has excellent thermal stability; when the lysable polysaccharide monooxygenase is used for synergistically hydrolyzing phosphoric acid swelling cellulose, microcrystalline cellulose and bagasse, the enzymolysis rate is increased, and the problems of poor thermal stability of the lysable polysaccharide monooxygenase, low enzymolysis rate of synergic cellulase and high LPMOs enzyme dosage are solved. The method has high practicability and can be widely applied to the enzymolysis process of various cellulosic substrates, so that the enzymolysis efficiency of the cellulosic substrates is remarkably improved.

The invention discloses a complex microbial inoculant for coupling straw decomposition and soil carbon sequestration and a preparation method thereof, the complex microbial inoculant can rapidly promote degradation of lignocellulose of straw in soil by compounding bacillus subtilis, bacillus licheniformis, pseudomonas aeruginosa and the like, provides a basic material energy source for activity of soil microorganisms, and has the advantages of simple preparation process and low cost. Meanwhile, corresponding microorganisms achieve long-term storage of organic carbon through humus stabilization and mineral substance combined carbon (MAOC) stabilization, the storage efficiency of the organic carbon is remarkably improved through nano Fe3O4, and under the synergistic effect of all strains in the complex microbial inoculant, the carbon loss of straw returning can be effectively reduced, the carbon immobilization capacity of soil is improved, and the soil quality is improved. The organic carbon content in the soil is increased.

The invention discloses a method for preparing recombinant III-type human collagen. The method comprises the following steps: constructing genetically engineered bacteria for expressing proline hydroxylase and full-length recombinant III-type human collagen; and culturing the genetically engineered bacteria to obtain the recombinant III-type human collagen, the proline hydroxylase is prepared from P4Ha2, P4Hb and vP4H; the ratio of the copy number of the P4Ha2 to the copy number of the P4Hb to the copy number of the vP4H to the copy number of the full-length recombinant III type human collagen is (1.80 to 2.20) to (1.80 to 2.20) to (0.80 to 1.60) to 1. The method can be used for large-scale production, the purity is high, and the production cost can be reduced; the prepared recombinant III-type human collagen is completely consistent with the full-length sequence of natural III-type human collagen, the hydroxyproline content of the recombinant III-type human collagen is basically consistent with that of the natural III-type human collagen, and the recombinant III-type human collagen has a triple-helix structure, has the biological activity of the natural III-type human collagen, and is free of immunogenicity and virus hidden danger.

The invention belongs to the technical field of protein engineering, and discloses a Brazilian sweet protein mutant and application thereof. According to the invention, lysine is used for replacing aspartic acid at the 29th site of Brazilian sweet protein, and / or arginine is used for replacing glutamic acid at the 36th site, and / or alanine is used for replacing aspartic acid at the 50th site, amino acid mutation at the sites is carried out to construct a Brazilian sweet protein mutant, and the Brazilian sweet protein mutant is subjected to heterologous expression in pichia pastoris GS115, so that the Brazilian sweet protein mutant is obtained. And carrying out sweetness performance evaluation on the expressed Brazilian sweet protein mutant. The sweetness property of the double mutant Asp29Lys-Asp50Ala protein is improved by 240% compared with the sweetness property of the Brazilian sweet protein before mutation.

The invention belongs to the technical field of microbiology and agricultural application thereof, and particularly relates to talaromyces sp., a microbial agent and application of the talaromyces sp. According to the invention, a fungus with antagonistic action on fusarium oxysporum is screened from the root of black nightshade, the fungus is identified as talaromyces, the inhibition rate of the strain on fusarium oxysporum is 68.4%, and pot experiment shows that the relative control effect of the strain on tobacco root rot is 61.1%; a field experiment result shows that the relative prevention effect of the strain on the tobacco root rot is 88.8%, the disease index of the tobacco root rot can be effectively reduced, large-area infection of pathogenic bacteria is inhibited to a certain extent, and meanwhile, the talaromyces sp. Strain screened by the invention can induce the activity of defense enzymes PPO, PAL and POD of tobacco seedlings, improve the disease resistance of the seedlings and protect the growth of tobacco plants. Therefore, talaromyces sp. Has great potential value in the aspect of preventing and treating tobacco root rot.

The invention discloses talaromyces purpurogenus, a preparation method and application in prevention and treatment of corn stalk rot, and provides a novel strain, namely talaromyces purpurogenus Jnf41, greenhouse control effect experiments show that the talaromyces purpurogenus Jnf41 can inhibit the scab area and the scab length of the corn stalk rot in greenhouse control effect determination, and the talaromyces purpurogenus Jnf41 can be used for preventing and treating the corn stalk rot. Infection of the corn stalk rot can be effectively inhibited, pathogenic bacteria are not prone to generating drug resistance, green prevention and control are implemented in the prevention and control process, and the biological preparation for resisting the corn stalk rot is prepared and has important significance on green prevention and control of the corn stalk rot.

The invention discloses a mesophilic carotenoid-producing recombinant yarrowia lipolytica strain as well as a construction method and application thereof, the recombinant strain exogenously expresses mesophilic original fatty acid desaturase (FAD3) and isocitrate dehydrogenase (IDH2) mutants from phaffia rhodozyma, and efficient synthesis under the condition of 30 DEG C is realized; secondly, a metabolic pathway of the recombinant strain is optimized based on a metabolic flow push-pull strategy, and isopentenyl pyrophosphate isomerase (IDI), farnesyl pyrophosphate synthase (ERG20), plant alkene dehydrogenase (CarB) and bifunctional plant alkene synthase (CarRP) are overexpressed, so that the flux of a precursor is increased; finally, gene multi-copy of astaxanthin synthase (crtS) and cytochrome P450 reductase (crtR) from phaffia rhodozyma is carried out, and the carotenoid production capacity of yarrowia lipolytica is further improved. The construction method of the mesophilic yarrowia lipolytica is simple, efficient synthesis of carotenoid can be better achieved at the higher temperature, the fermentation cost is greatly reduced, and the industrial production potential is achieved.

The invention relates to the field of enzyme engineering, in particular to modified xylanase, a DNA (deoxyribonucleic acid) molecule, a recombinant plasmid and a transfected cell. The modified xylanase protected by the application is formed by replacing a deletion segment of a receptor enzyme with a substitution segment, the receptor enzyme is wild type xylanase, the sequence of the receptor enzyme is SEQ ID NO: 1, the deletion segment comprises a carbohydrate binding module of the receptor enzyme, the substitution segment is a carbohydrate binding module of a donor enzyme, the donor enzyme is a carbohydrate active enzyme, and the donor enzyme is a carbohydrate active enzyme. The sequence of the donor enzyme is one of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5. The carbohydrate binding module of the carbohydrate active enzyme with relatively high activity is replaced into the receptor enzyme, so that the binding capacity of the receptor enzyme and the carbohydrate is improved, and the activity of the receptor enzyme is improved.

The invention discloses construction and application of an L-malic acid producing issatchenkia orientalis strain, and belongs to the technical field of microorganisms and the technical field of microbial fermentation and metabolic engineering. Through a metabolic engineering means, a cytoplasm metabolic pathway of an issatchenkia orientalis strain is transformed, and a pyruvate decarboxylase coding gene, glycerol dehydrogenase and lactic dehydrogenase are knocked out; a pyruvate carboxylase gene derived from aspergillus flavus, a malate dehydrogenase gene derived from corynebacterium glutamicum and phosphoenolpyruvate carboxylase derived from escherichia coli are screened and integrated, so that a cytoplasm rTCA cycle synthesis pathway is enhanced; inserting a dicarboxylic acid external transporter gene from schizosaccharomyces cerevisiae at a neutral site GD21, and knocking out endogenous dicarboxylic acid internal transporter; the L-malic acid yield of the modified yeast strain in the fermentation process is remarkably increased and reaches 140.16 g / L. The engineering strain provided by the invention has good industrial application potential in efficient L-malic acid production.

The invention discloses a salt-tolerant zygosaccharomyces rouxii strain with high ethanol yield and application thereof in preparation of soy sauce, and belongs to the technical field of microorganisms, the salt-tolerant zygosaccharomyces rouxii strain SS-y178 is preserved in the China Center for Type Culture Collection on July 15, 2024, the preservation number is CCTCC NO: M 20241563, the preservation address is Wuhan University, Wuhan, China, the salt-tolerant zygosaccharomyces rouxii strain is applied to fermentation production of soy sauce, and the salt-tolerant zygosaccharomyces rouxii strain can be used for preparing the soy sauce. The method has the advantages of high yield of ethanol, promotion of fermentation fat production and increase of the types of lipid substances, has good effects in promotion of fat increase and aroma enhancement of soy sauce, and has wide application prospects.

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention provides an application of an MET14 gene or an MET14 protein in improving the freezing resistance of saccharomyces cerevisiae. It is found for the first time that through overexpression of the MET14 gene or the MET14 protein, the freezing resistance of the saccharomyces cerevisiae can be remarkably improved, the saccharomyces cerevisiae can normally ferment and grow in frozen products, the excellent physiological activity of the saccharomyces cerevisiae in the frozen products is fully exerted, and then the quality of the frozen products is remarkably improved.

The invention belongs to the technical field of microbial fermentation and food engineering, and discloses a fermentation method for enhancing the production capacity of schizochytrium limacinum phospholipid DHA and application, schizochytrium limacinum is subjected to fermentation culture for 32-60 h, and the addition of an organic nitrogen source and phosphate is optimized in the fermentation culture process; the formula of a seed culture medium for culturing schizochytrium limacinum is not changed, and only the formula of a fermentation culture medium for schizochytrium limacinum is changed. The invention provides a fermentation strategy for enhancing the production capacity of the schizochytrium limacinum phospholipid DHA, and the schizochytrium limacinum can obtain the phospholipid DHA with higher content by optimizing an organic nitrogen source and phosphate in a schizochytrium limacinum fermentation culture medium. According to the obtained schizochytrium limacinum grease, the total DHA content is obviously increased compared with that of a traditional fermentation strategy, and the proportion of phospholipid DHA is also remarkably increased. The regulation and control method is applied to preparation of schizochytrium limacinum algae powder, the schizochytrium limacinum algae powder rich in phospholipid DHA is obtained, and application of the schizochytrium limacinum algae powder in the field of aquatic feed is facilitated.

The invention relates to the technical field of health care products, and discloses kidney function health wine based on traditional Chinese medicine fermentation and a preparation method of the kidney function health wine. 6-10 parts of cistanche deserticola; 9-30 parts of radix astragali and 6-12 parts of fructus lycii; the fermentation strain comprises lactobacillus acidophilus, lactobacillus plantarum, bifidobacterium longum subsp. Longum, saccharomyces cerevisiae and bacillus subtilis, and the fermentation strain comprises the following strains: lactobacillus acidophilus JYLC-374, lactobacillus plantarum JYLC-482, bifidobacterium longum subsp. Longum JYBB-163, saccharomyces cerevisiae JYSC-710 and bacillus subtilis JYBS-312. By adopting the technical scheme that a traditional Chinese medicine compound formula is combined with specific probiotic fermentation, the technical effects of enhancing the bioavailability of active ingredients and promoting kidney function regulation are achieved, and the dissolution rate and the biotransformation rate of herbal medicine active substances are improved.