8270results about "Fungi" patented technology

The invention relates to ice wine yeast with high fermentation activity and outstanding aroma-producing characteristics and application thereof. The ice wine yeast is yeast (Saccharomyces cerevisiae) YSL16010, and the bacterial strain has been preserved in China General Microbiological Culture Collection Center on April 18, 2016, with a preservation number of CGMCC No.12349. The bacterial strain YSL16010 is obtained by separating and screening Vidal grapes from a Huanren producing area of Liaoning at a temperature of 16 DEG C in a natural fermentation process, and the ice wine wine-making yeast having good making characteristics and highly producing esters is obtained through a series of screening and identifications and small pilot scale fermentation. The ice wine wine-making yeast YSL1610 highly producing esters is used for fermenting and producing ice wine, so that the yield of volatile acid is effectively controlled while the standards of the grape wine producing countries can be met, and therefore, the content of aromatic compounds such as esters is remarkably increased. The bacterial strain can be applied to ice wine brewing industry, and can play an important role in improving the quality of the ice wine and bringing advantages of the grape producing areas into play.

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

This invention provides an in vitro cell-free protein synthesis system, its reagent kit, and its applications, belonging to the field of protein synthesis technology. This invention improves an in vitro cell-free protein synthesis system based on Kluyveromyces lactis by adding exogenous L-arabinose, significantly enhancing the system's protein synthesis capacity. Furthermore, it provides a more efficient and higher-throughput in vitro protein synthesis reagent kit and a method for synthesizing exogenous proteins. The improvements provided by this invention can be achieved without molecular modification, are simple and convenient to operate, and save costs.

The invention discloses a recombinant XVII type collagen engineering bacterium as well as a preparation method and application thereof, and belongs to the technical field of genetic engineering, and the recombinant XVII type collagen engineering bacterium is realized through three steps of designing and optimizing collagen, improving translation through codon optimization and selecting a vector for cloning. By optimizing the amino acid sequence and the codon, the expression efficiency and the stability of the recombinant XVII type collagen in engineering bacteria are improved. The optimization not only improves the production efficiency of the protein, but also reduces the production cost. The recombinant XVII type collagen has potential in the medical field, especially in the fields of skin repair, wound healing, aging resistance and the like. Meanwhile, in the fields of beauty and biological materials, collagen is widely applied to products such as masks and skin care products as a basic component, and can promote skin cell regeneration and enhance skin elasticity and repair capacity.

The invention relates to the technical field of synthetic biology, in particular to recombinant XVII type collagen as well as a preparation method and application thereof.The amino acid sequence of the recombinant XVII type collagen comprises a sequence shown in any one of SEQ ID NO.1-SEQ ID NO.3. The recombinant XVII type collagen can be highly expressed in an escherichia coli and pichia pastoris system, the expression system is simple, the cost is low, and the recombinant XVII type collagen is suitable for industrial production. The yield is high; the biological activity is good. The protein can be compounded with other proteins to form raw materials of tissue engineering products, cosmetics, health care products or medicines, so that the protein can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like.

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention relates to a diacylglycerol acyltransferase mutant and application thereof in synthesis of triacylglycerol from saccharomyces cerevisiae, and belongs to the technical field of enzyme engineering. Diacylglycerol acyltransferase is a rate-limiting step of a synthetic route of saccharomyces cerevisiae triacylglycerol (TAG), and at present, a research on a DGA1 mutant for efficiently synthesizing TAG is lacked, and a research on the aspect of a DGA1 catalytic mechanism is also lacked. The DGA1 mutant capable of effectively improving TAG synthesis is obtained by screening in combination with a directed evolution technology, and a result shows that 282-site mutation of diacylglycerol acyltransferase can significantly increase the accumulation amount of TAG. Meanwhile, the catalytic mechanism of the DGA1 is preliminarily explored by combining protein structure prediction, molecular docking and molecular dynamics simulation, and a foundation is laid for deeply analyzing the catalytic mechanism of the DGA1. The recombinant saccharomyces cerevisiae is also constructed based on the mutant, so that the TAG synthesis is improved, and meanwhile, the relative proportion of C18: 1 in total fatty acids is remarkably increased.

The invention discloses an aspergillus niger recombinant bacterium AnCT-XynB-K-agdA and application thereof, and belongs to the technical field of microbiology and bioengineering. According to the invention, Aspergillus niger AnCat is taken as an expression host, firstly, an acid protease regulatory factor prtT is knocked out to obtain a defective strain, and then different chaperonins are subjected to fusion expression by optimizing integration sites of a laccase lcc9 expression cassette, so that the high-expression laccase chaperonin is obtained. According to the method, an expression vector pC3-5 'agdA-PcitA-XynB-L-lcc9-hph-3' agdA is constructed, a recombinant bacterium AnCT-XynB-K-agdA is further obtained, the enzyme activity of the extracellular laccase subjected to shake flask fermentation reaches 1821.2 U / L, and finally, the recombinant efficient expression of the laccase Lcc9 in aspergillus niger is realized.

The invention relates to the technical field of ecological restoration, in particular to a preparation method of novel vegetation concrete greening additive AB bacteria, which comprises the following steps: step (1) screening and activating double bacteria; (2) preparing an inner core structure; (3) loading double bacteria; (4) coating a shell layer; (5) loading a functional matrix; and (6) preparing the vegetation concrete greening additive AB bacteria. According to the method, a multi-layer and function-synergistic microorganism-seed-carrier composite system is constructed and used for surface concrete attachment of the high steep slope, and a plant-microorganism symbiotic system with physical isolation, chemical buffering and nutrition self-sustaining capacities is actively constructed for plant seeds and functional microorganisms in a strongly alkaline macroscopic environment; and finally, the long-acting ecological stability of the high and steep slope is realized.

The invention relates to the technical field of microorganisms and application of the microorganisms, and discloses a Crystalomyces hyper bacterium and application of the Crystalomyces hyper bacterium. The Crystalomyces hyricus is obtained by being separated from hypericum flower buds of hypericum persicum belonging to the ligustraceae in the Fangshan, Dali, Yunnan, China, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 20, 2025, and the preservation number is CGMCC No.42087. The Crystalomyces hyricus has the advantages that the Crystalomyces hyricus can be used for preparing the high-efficiency and high-efficiency culture medium for the high-efficiency and high-efficiency culture medium for the high-efficiency and high-efficiency culture medium for the high-efficiency and high-efficiency culture medium; according to the present invention, the experiment results show that the extraction liquid of the Crystalomyces hyper bacteria has the anti-cancer effect, such that the extraction liquid can be used for preparing the anti-cancer drug.

The invention discloses Mucor irregularia and application thereof, the strain number of the Mucor irregularia is JSAFC 3408, the Mucor irregularia is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 6, 2025, and the preservation number is CGMCC No.41982. The Mucor irregularia has the advantages that the Mucor irregularia has the advantages that the Mucor irregularia can be used for preparing the Mucor irregularia; according to the application, the soil around the pepper is separated and purified, so that the Mucor irregularia is obtained. The strain can inhibit the growth of the huanghuai glossospora, promote the development of the pepper and regulate the growth of plants, and has great significance for the development of a biocontrol fungicide for preventing and treating the pepper root rot in the future.

The invention provides a self-assembled hypha flexible carbon electrode material and a preparation method and application thereof, and belongs to the technical field of battery packs. The preparation method of the self-assembled hypha flexible carbon electrode material comprises the following steps: treating basswood with white rot fungus mycelia, crushing and roasting to obtain porous basswood / hypha composite carbon powder; and putting the porous basswood / hypha composite carbon powder and a carbon nanotube into a sterilized conical flask containing a potato glucose culture solution, inoculating gloeophyllum trabeum, performing activation culture, adding a dopamine hydrochloride aqueous solution, performing polymerization after ultraviolet irradiation, taking out and tabletting to obtain the self-assembled hypha flexible carbon electrode material. In the preparation process of the self-assembled hypha flexible carbon electrode material, the flexible carbon electrode material is synthesized by taking porous basswood / hypha composite carbon powder as a matrix, taking gloeophyllum trabeum hypha as an adhesive, taking polydopamine as a reinforcing agent and adding carbon nanotubes as a conductive reinforcing material, so that the technical problem of poor cycle performance of a lithium ion battery is solved.

The invention relates to a preparation method of a rice leavening with a scalp care effect and a product and application thereof, the preparation method comprises the following steps: mixing a rice raw material, a plant raw material, water, a magnesium salt, a sodium salt and Tween, sterilizing to obtain a fermentation medium, inoculating a compound microbial agent into the fermentation medium, and fermenting to obtain the rice leavening with the scalp care effect. After the fermentation liquid is finished, sterilizing and filtering to obtain a rice leavening; wherein the compound microbial agent comprises a first microbial strain and a second microbial strain; the strains in the first microbial strain comprise a combination of streptococcus thermophilus, bifidobacterium breve and a plant lactobacillus YK-L002 strain; and the second microbial strain comprises a trichosporon YK-S004 strain. According to the invention, specific strains are subjected to compounding and combined fermentation, and the fermentation product filtrate obtained after fermentation treatment of four bacteria has excellent hair care and nourishing effects, and can effectively condition the scalp micro-ecological environment and nourish the hair quality.

The invention belongs to the field of biosynthesis, and in particular relates to LBD (Levandanenediol Pyrophosphate) synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, the sclareol biosynthesis yield is generally low, LSLPPs mutant containing at least one mutation of D730L, D374L, N674L or G379M is obtained by performing mutation and optimization on LSLPPs pyrophosphate synthase, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, and the yield of a 5L fermentation tank reaches 26.11 g / L and is greatly improved compared with the prior art.

The invention discloses Mucor abundans and application of the Mucor abundans, the strain number of the Mucor abundans is JSAFC 2484, the Mucor abundans is preserved in the China General Microbiological Culture Collection Center on May 6, 2025, and the preservation number is CGMCC No. 41979. The Mucor abundans can be applied to the field of biological engineering. The biocontrol bacterium Mucor abundans JSAFC 2484 provided by the invention can effectively inhibit the growth of Fusarium equiseti, the inhibition rate of plate confrontation culture reaches 68.52%, meanwhile, extracellular metabolites secreted by the biocontrol bacterium Mucor abundans JSAFC 2484 can also significantly reduce the growth of the Fusarium equiseti, when the concentration of the extracellular metabolites of the JSAFC 2484 is 10 mL / 100 mL, the inhibition rate of the Fusarium equiseti reaches 76.35%, and when the concentration of the extracellular metabolites of the JSAFC 2484 is 100 mL / 100 mL, the inhibition rate of the Fusarium equiseti reaches 79.05%. Meanwhile, the plant growth promoting effect is achieved on pepper plants.

The invention provides a clostridium endophyllum engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of biology, compared with a wild type strain, the clostridium endophyllum engineering bacterium can only produce acetic acid in a low-pH environment under the condition of taking CO2 and H2 as raw materials, and does not generate by-products such as ethanol, lactic acid, 2, 3-butanediol and the like. Besides, the fermentation liquor of the clostridium endophyllum engineering bacteria can be directly used as an acidic substrate to adjust the pH value of downstream yeast fermentation liquor, meanwhile, automatic addition of a carbon source is achieved, zero addition of inorganic salt in the cascade conversion process from CO2 and H2 to grease or yeast protein is achieved, and the economical efficiency and environmental friendliness of the whole process are improved.

The invention belongs to the field of biosynthesis, and particularly relates to sclareol synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, sclareol biosynthesis yield is generally low, sclareol synthase is mutated and optimized, an SsScs mutant containing at least one mutation of Y491F, G307L or N269F is obtained, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, and the yield of a 5L fermentation tank reaches 21.13 g / L and is greatly improved compared with the prior art.

The invention provides recombinant IV type collagen and application thereof, and relates to the technical field of protein engineering. In the invention, an amino acid residue repetitive unit with good biological activity and stability in the human-derived IV-type collagen is found, and the amino acid residue repetitive unit is used as a core for repeating and constructing the recombinant IV-type collagen, so that the novel human-derived recombinant IV-type collagen with biological activity is obtained. The human-derived recombinant IV-type collagen provided by the invention is a small-molecular-weight protein, is easy to absorb by a human body, is good in safety, can remarkably promote secretion of collagen, also has the effects of promoting cell adhesion and cell migration, and has a good application prospect in the aspects of basement membrane repair and remodeling. A new functional component is provided for products for repairing and tightening the skin, resisting aging, resisting wrinkles, preserving moisture and the like.

The present invention provides a method for producing extracellular vesicles, the method including a step for passing a crushed liquid of plants, mushrooms, or microalgae through a depth filter, and a step for subjecting the crushed liquid after passing through the depth filter to ultrafiltration treatment, the depth filter being composed of filaments.

The invention discloses a pichia pastoris engineering bacterium for synthesizing bakuchiol from de novo by converting methanol as well as construction and application of the pichia pastoris engineering bacterium. A heterologous p-coumaric acid synthesis pathway is introduced to neutral sites of different chromosomes of pichia pastoris, a high-yield p-coumaric acid strain is obtained through gene knockout, overexpression or heterologous expression of tyrosine and phenylalanine synthesis pathway key genes of a shikimic acid pathway, a heterologous bakuchiol biosynthesis pathway is introduced on the basis, and a high-yield p-coumaric acid strain is obtained. After overexpression of bakuchiol synthetase, endogenous overexpression or heterologous expression of MVA pathway key genes and exogenous acetyl coenzyme A supply pathway genes, methanol concentration optimization and bacterial strain His4 gene supplementation, the yield of bakuchiol is effectively increased to 91.2 mg / L and is increased by 59.8 times compared with that of an initial bacterial strain, and the yield of bakuchiol in a 15L fermentation tank reaches 692.8 mg / L. The method has the characteristics of high conversion efficiency, low production cost, convenience in preparation, wide industrial application prospect and the like.

The invention discloses recombinant IV-type collagen as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. The amino acid sequence of the recombinant IV type collagen comprises a plurality of repetitive units, and the repetitive units are shown as SEQ ID NO. 1. The recombinant IV type collagen fragment is derived from human IV type collagen, heterologous amino acid residues are not introduced, and rejection and sensitization risks are avoided; meanwhile, the hydrogel also has good biological activity, including cell proliferation promoting activity and cell adhesion promoting activity, and is good in biocompatibility. In addition, the preparation method of the recombinant IV-type collagen is simple and easy to operate, and the recombinant IV-type collagen with high purity and stability can be obtained through the fermentation and purification method. Therefore, the recombinant IV type collagen disclosed by the invention has a relatively good application prospect.

The invention discloses a culture method for increasing the protein content of aspergillus niger mycelia by using corn steep liquor as a culture medium, and belongs to the technical field of biological fermentation. Aiming at insufficient resource utilization of corn steep liquor and substitute protein production requirements, a corn processing by-product corn steep liquor is creatively adopted to directly replace a traditional PDB culture medium, and the mycelium protein content is remarkably increased by optimizing a fermentation system. Results show that the corn steep liquor is used as a culture medium, aspergillus niger with the preservation number of CCTCC M 2013703 is inoculated, and aspergillus niger mycelium protein can be obtained through fermentation. The protein content of the mycelium obtained by the process disclosed by the invention reaches 50.62-53.56%, and the total amino acid content is increased by 64-76%. According to the method, high-value conversion of agricultural corn byproducts is realized, and an efficient and low-cost industrial solution is provided for alternative protein production.

The invention discloses NnLuz and NnH3H protein mutants and an application of the NnLuz and NnH3H protein mutants in enhancing the bioluminescence intensity of fungi or plants. According to the method disclosed by the invention, core enzymes NnLuz and NnH3H in a fungus bioluminescence pathway are optimized through rational design, and the catalytic efficiency and the luminous intensity of a bioluminescence system are remarkably improved. Experiments prove that 1) the luminous intensity of the NnLuz [delta] N19v2 (I103T / L167K double mutant) in yeast and plants is remarkably superior to that of a reported NnLuzv4 mutant and is 3-4 times that of the reported NnLuzv4 mutant, 2) the catalytic efficiency of the NnH3Hv3 (K307T mutant) is improved by more than 2 times, the luminous stability is remarkably enhanced, and 3) when the NnLuz [delta] N19v2 and the NnH3Hv3 are cooperatively expressed, the luminous intensity of the system reaches the optimal level, and the self-luminous plant with remarkably enhanced luminescence is created.

The invention relates to a recombinant I-type humanized collagen with crosslinking property as well as a preparation method and application thereof, and belongs to the technical field of recombinant proteins. The invention relates to recombinant I-type humanized collagen with crosslinking property. The amino acid sequence of the recombinant I-type humanized collagen comprises an amino acid sequence as shown in SEQ ID No.1 or a derivative amino acid sequence with the sequence identity of more than 90% with the sequence as shown in SEQ ID No.1; the recombinant I-type humanized collagen has a triple helix structure. A complete triple helix structure can be efficiently expressed, and the hydrogel has efficient cross-linking capability, so that the hydrogel can be efficiently and stably formed through cross-linking.

The invention discloses mucor oosperm and application of the mucor oosperm, the bacterial strain number of the mucor oosperm is JSAFC 2483, the mucor oosperm is preserved in the China General Microbiological Culture Collection Center on May 6, 2025, and the preservation number is CGMCC No.41980. The mucor oosperm has the advantages that the mucor oosperm can be used for preparing the mucor oosperm; the biocontrol bacterium Mucor moelleni JSAFC 2483 provided by the invention can effectively inhibit the growth of Fusarium equiseti, the inhibition rate of plate confrontation culture reaches 54.90%, meanwhile, extracellular metabolites secreted by the biocontrol bacterium Mucor moelleni JSAFC 2483 can also significantly reduce the growth of the Fusarium equiseti, when the concentration of the extracellular metabolites of the JSAFC 2483 is 10 mL / 100 mL, the inhibition rate of the Fusarium equiseti reaches 53.98%, and when the concentration of the extracellular metabolites of the JSAFC 2483 is 100 mL / 100 mL, the inhibition rate of the Fusarium equiseti reaches 56.64%. Meanwhile, the plant growth promoting effect is achieved on pepper plants.

The invention discloses a multifunctional talaromyces sp. Fungus RB-2 for preventing and controlling ginger stem rot and application of the multifunctional talaromyces sp. Fungus RB-2, and belongs to the technical field of microorganisms. The talaromyces sp. Fungus RB-2 is preserved in the China Center for Type Culture Collection, the preservation number of the talaromyces sp. Fungus RB-2 is CCTCC NO: M 20251722, and the preservation date of the talaromyces sp. Fungus RB-2 is July 28, 2025. The talaromyces sp. Strain RB-2 with the effect of inhibiting the growth of pathogenic bacteria is separated and purified, and the strain RB-2 has remarkable antagonistic activity on ginger stem rot pathogenic bacterium group pythium aphanidermatum and various economic crop pathogenic bacteria and has the potential of being developed into a biocontrol inoculant. The talaromyces sp. Strain RB-2 also has phosphorus dissolving and growth promoting capabilities, can remarkably promote the growth of ginger, and has the potential of preparing a soil conditioner and a microbial fertilizer, so that the micro-ecology of soil is improved, and the productivity of crops is enhanced.

The invention discloses a yellow flammulina velutipes ZJJZG002 and InDel marker with high resistance and high brittleness and application of the yellow flammulina velutipes ZJJZG002 and InDel marker, and relates to the technical field of edible mushroom cultivation, the preservation number of the yellow flammulina velutipes is CGMCC NO.41174, and the yellow flammulina velutipes is classified and named as flammulina velutipes. The strain ZJJZG002 takes yellow flammulina velutipes F24 and white flammulina velutipes W1 as parents, the parent materials are subjected to ultraviolet mutagenesis treatment and then are subjected to single spore isolation, hybridization (a hybrid combination XB51 * YU19) and fruiting tests, and the strain ZJJZG002 is not prone to umbrella opening, rich in mushroom fragrance, crisp and refreshing in taste, neat in fruiting, suitable for production in various cultivation modes and capable of injecting a new seed source for the domestic flammulina velutipes market.

The invention provides Aspergillus tubingensis HNZY02, a method for producing enzymes by the Aspergillus tubingensis HNZY02 and application of the Aspergillus tubingensis HNZY02, and belongs to the technical field of microorganisms, the Aspergillus tubingensis HNZY02 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.42148. The aspergillus tubingensis HNZY02 shows excellent cellulase and xylanase synthesis capability, can synergistically and efficiently degrade main structural components such as cellulose, hemicellulose and lignin in tobacco stems, and improves the safety and application value of cigarettes while breaking the compact structure and increasing the reducing sugar content.

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The invention provides a method for producing fragrance through co-culture of gliocladium neurospora and lachnum brachytrichum, and relates to the technical field of microorganisms. The method for producing fragrance by co-culture of the gliocladium neurosporum and the lachnum brachytrichum comprises the following steps: S1, strain activation: respectively inoculating the gliocladium neurosporum and the lachnum brachytrichum into a PSA solid culture medium for activation culture; s2, co-culture inoculation and culture: inoculating the activated gliocladium neurospora and lachnum brachytrichum in the step S1 into a PSA solid culture medium in the same culture container by adopting a five-point confrontation method; and S3, volatile matter collection and analysis: from the second day after inoculation, collecting headspace volatile matters of the culture every 48 hours by adopting a headspace solid-phase microextraction method, and then identifying the co-culture specific volatile organic compounds. Five kinds of specific VOCs which are not cultured independently can be induced to be generated, and the fragrance diversity is enriched; the method is stable and repeatable, the product contains clear aroma components, and an efficient and green new strategy is provided for natural perfume development.