45 results about "Exogenous enzymes" patented technology

The application discloses a method for preparing lactic acid by using a combined strain, and belongs to the technical field of microbial fermentation. Bacillus subtilis The combined strain comprises Bacillus subtilis Lactiplantibacillus pentosus LB9.The method for preparing lactic acid comprises the following steps: (1) preparing raw materials and strains, wherein the raw materials are white spirit lees; (2) mixing Bacillus subtilis and Lactobacillus pentosus after being activated respectively to form a combined strain, inoculating the combined strain into a culture medium taking the white spirit lees as the only fermentation substrate and without adding exogenous enzyme preparations and exogenous auxiliary materials, and performing fermentation; in the fermentation process, a staged oxygen supply strategy is adopted, namely, performing aerobic fermentation first and then performing anaerobic fermentation; and (3) collecting lactic acid from a fermentation product.The application realizes high-value utilization of lignocellulose waste, effectively coordinates metabolic conflicts among strains, and solves the problem of efficient conversion of lignocellulose lees into lactic acid.

The application discloses a high-activity UGT1A1 enzyme based on tree shrew and a feed composition for enhancing alcohol metabolism. The catalytic efficiency of the recombinant tree shrew UGT1A1 enzyme on ethanol is significantly higher than that of human isozyme. The core of the application is to provide a complex feed system containing the enzyme, which solves the key bottleneck of poor stability and low delivery efficiency of exogenous enzymes in application through the synergistic effect of enzyme stabilizers, cell penetration enhancers, enteric coatings and coenzyme supply agents. Animal experiments show that the composition can safely and effectively accelerate in vivo alcohol elimination and reduce blood ethanol concentration, and can be used for developing alcoholism products, acute alcoholism treatment drugs and alcoholic liver disease prevention preparations.

This invention discloses a method for improving the flavor of sweet rice wine through low-temperature fermentation and its brewing technique, relating to the field of winemaking technology. First, a short-term temperature increase (28-30℃) is implemented in the early stages of fermentation to create an optimal environment for efficient saccharification and flavor precursor accumulation in Rhizopus. Subsequently, a low-temperature stage (15-20℃) is initiated, under which slow primary fermentation and flavor maturation, primarily driven by yeast, take place. The "staged temperature-increase saccharification + low-temperature fermentation" process provided by this invention does not rely on psychrophilic strains or exogenous enzyme additives; it can be achieved through optimization of process parameters. This method not only successfully solves the core problem of insufficient saccharification kinetics at low temperatures, ensuring the smooth progress of the fermentation process, but also significantly improves the sensory quality of the final sweet rice wine (more complex and harmonious flavor), as well as the content of umami and sweet amino acids. Furthermore, this process is highly controllable and suitable for large-scale production, providing a novel technological path for the green and efficient brewing of high-quality sweet rice wine.

The invention discloses an enzyme-activated fluorescent molecular precursor as well as a preparation method and application thereof. The enzyme-activated fluorescent molecular precursor has a chemical structure as shown in a formula I which is described in the specification. The invention develops an enzyme-activated fluorescent molecular precursor which can be used for real-time imaging of tumors by targeting exogenous enzyme to the tumors and specifically activating a probe at the tumors; according to the invention, the exogenous enzyme is introduced as an activation trigger, and normal cells do not have the exogenous enzyme, so that the problem of co-expression of the endogenous enzyme in non-tumor tissues can be avoided, background fluorescence signals can be reduced to the greatest extent, and the imaging specificity and accuracy are remarkably improved.

The present disclosure concerns a process for fermenting a biomass with a reduced dose of a purified exogenous enzyme (which can be, for example a purified exogenous glucoamylase). The process comprises contacting a biomass (which may comprise starch) with a recombinant yeast host cell. The recombinant yeast host cell has a genetic modification for expressing a heterologous polypeptide having starch or dextrin hydrolase activity (which may be, for example, from a glucoamylase). The nucleic acid molecule encoding the heterologous polypeptide having starch or dextrin hydrolase activity comprises allowing the secretion of the heterologous polypeptide.

The present application relates to the field of food technology, and discloses a low-bitterness wolfberry original syrup and a preparation process thereof. The original syrup is prepared from the following raw materials in parts by weight: 80-90 parts of fresh wolfberry fruit, 5-10 parts of germinated fava, 5-10 parts of germinated barley, 0.3-0.6 parts of medium-chain triglyceride, 1.0-2.0 parts of concentrated red date juice, 0.05-0.1 parts of ascorbic acid, 0.02-0.05 parts of citric acid, and 0.01-0.05 parts of saltwort extract. The present application efficiently reduces the bitterness of the wolfberry original syrup through a full natural biological transformation pathway without adding exogenous enzyme preparations or introducing chemical adsorbents, fundamentally avoiding chemical addition and clean label problems, and simultaneously achieving the synchronous transformation of various types of bitter substances in wolfberry. The preparation process of the present application completely retains the heat-sensitive functional factors and fresh fruit aroma of wolfberry, and the nutritional integrity and flavor reduction degree of the product are significantly better than those of similar products on the market.

The invention discloses a multifunctional active peptide from mackerel viscera and application of the multifunctional active peptide. The mackerel viscera extract is prepared by adopting a single exogenous enzyme hydrolysis method, the extract has small molecule peptides with ACE inhibitory activity, three ACE inhibitory peptides are obtained by further identification and screening, the amino acid sequences are respectively GPPVPGP, GPMGPMGP and SPGPVGMP, and the half inhibitory concentration IC50 values of the ACE inhibitory peptides are respectively 80.84 + / -1.12 mu g / mL, 177.41 + / -3.26 mu g / mL and 163.25 + / -3.46 mu g / mL. Wherein the heptapeptide GPPVPGP with the optimal activity also has the function of removing DPPH free radicals and hydroxyl free radicals, and can be used for adjuvant therapy for reducing hypertension or applied to other fields.

The invention provides a biosynthesis method for efficiently producing aromatic non-natural amino acid and site-specific modified recombinant protein, an engineering strain and application of the engineering strain. Specifically, the invention provides an engineering bacterium, and genes of one or more exogenous enzymes selected from the following groups are introduced into the engineering bacterium: threonine aldolase, threonine deaminase and transaminase; and threonine aldolase, threonine deaminase and transaminase are simultaneously expressed in the engineering bacterium. The engineering bacterium disclosed by the invention can be used for efficiently synthesizing aromatic non-natural amino acid through whole-cell catalysis or fermentation conversion. More importantly, the synthetic route provided by the invention can be synergistically coupled with a gene codon extension technology in the same engineering bacterium, and is used for high-efficiency expression of recombinant target protein modified by aromatic non-natural amino acid at a fixed point.

The invention discloses a method for increasing the content of sulforaphane in broccoli through magnetic field assisted blanching, and belongs to the technical field of food processing. The method for improving the content of sulforaphane in broccoli through magnetic field assisted blanching comprises the following steps: (1) preparing raw materials; (2) magnetic field assisted blanching pretreatment; (3) cooling and structure keeping treatment; (4) carrying out enzymatic incubation based on a magnetic field-assisted blanching regulation state; and (5) obtaining the broccoli product with high sulforaphane content. Exogenous enzymes, chemical reagents or additives are not introduced in the process, and the activity of endogenous myrosinase and the catalytic hydrolysis path of the endogenous myrosinase are regulated by synchronously applying a physical regulation means in the blanching pretreatment process, so that the generation level of sulforaphane in a treated sample is improved. The method is suitable for a food processing system, has the advantages of mild process conditions, strong controllability, high safety and easiness in industrial amplification, and has a wide application prospect in the field of food processing.

This invention discloses a fully solid-state fermentation process for Astragalus membranaceus vinegar, specifically relating to the field of solid-state vinegar brewing technology. The process uses sorghum, rice husks, rice bran, wheat bran, Astragalus membranaceus, and Daqu (a type of starter culture) as raw materials, without adding any exogenous enzymes or chemical products. The process includes the following steps: S1. Astragalus membranaceus slices are diagonally sliced ​​and drained to a moisture content of 18%-22%; S2. Sorghum is steamed at normal pressure, with Astragalus membranaceus slices added after 20-25 minutes of steaming, and steaming continues for a total time of 40-60 minutes; S3. Sorghum undergoes variable-temperature alcoholic fermentation with rice bran, first-grain rice husks, and a portion of Daqu, without Astragalus membranaceus slices participating in this stage; S4. The mash is mixed with the remaining Daqu, wheat bran, second-grain rice husks, and softened Astragalus membranaceus slices under moist heat, and the moisture content is adjusted to 60%-65% before acetic acid fermentation, with the main fermentation temperature at 39℃-41℃; S5. The mash is aged in a sealed container with intermittent aeration; S6. Vinegar is obtained by gradient temperature leaching. This invention achieves simultaneous fermentation and self-degradation of Astragalus membranaceus fiber and in-situ conversion of its functional components through process timing control and physical parameter optimization.

The invention discloses a processing method for improving clarity of instant tea, and belongs to the technical field of deep processing of tea leaves, the method mainly comprises the following steps: extracting and roughly filtering tea leaves to obtain primary tea soup; adding a compound enzyme into the primary tea soup, carrying out enzymolysis treatment, and then carrying out enzyme deactivation; sequentially performing ceramic membrane microfiltration, ultrafiltration membrane fine filtration and nanofiltration membrane concentration on the treated tea soup; quickly cooling the obtained concentrated solution, standing and aging at low temperature, and centrifuging at high speed to obtain supernatant liquid; and finally, drying to obtain the instant tea powder. According to the method, various substances causing turbidity in tea soup are systematically removed through the synergistic effect of exogenous enzymolysis, three-stage membrane integrated purification and low-temperature physical coagulation acceleration, and the method has the beneficial effects that the final clarity and cold water solubility of an instant tea product can be effectively improved, and the storage stability of the product is improved.

The application provides a vinegar residue-based single-cell protein feed and a preparation method and application thereof, and belongs to the technical field of feed preparation.The application uses vinegar residue as raw material, adopts solid-state fermentation of Aspergillus, Candida utilis and Saccharomyces cerevisiae in combination with composite enzymes, utilizes the synergistic effect among the strains and the enzymolysis effect of the exogenous enzymes, efficiently degrades the fibrous substances in the vinegar residue, and converts the fibrous substances into microbial proteins, so that the crude protein content of the prepared vinegar residue-based single-cell protein feed is increased from 5% to 8% of the raw material to 20% to 25%, and the total fibrous substance degradation rate is greater than or equal to 60%. The vinegar residue-based single-cell protein feed produced by the application has good feeding value, can effectively replace part of the protein raw materials such as soybean meal, improves the production performance of beef cattle, reduces the breeding cost, realizes high-value utilization of vinegar residue resources, and has good popularization and application prospect.

Provided in the present invention are a biosynthesis method for efficiently producing aromatic unnatural amino acids and site-specifically modified recombinant proteins, an engineered strain, and use thereof. Specifically, the present invention provides an engineered bacterium. Genes of exogenous enzymes, threonine aldolase and threonine deaminase, are introduced into the engineered bacterium, and the engineered bacterium expresses both the threonine aldolase and the threonine deaminase. The engineered bacterium of the present invention enables efficient synthesis of aromatic unnatural amino acids by means of whole-cell catalysis or fermentation transformation. The synthetic route of the present invention can be synergistically coupled with gene codon extension technology in the same engineered bacterium for efficient expression of recombinant target proteins site-specifically modified with aromatic unnatural amino acids.

A subject-matter of the present invention is a method for the dyeing of keratin fibres employing a composition C comprising: (i) indican of formula (I) and / or a powder containing it; (ii) at least one exogenous enzyme of hydrolase type; (iii) at least one exogenous enzyme of oxidase type; and (iv) water.

The invention discloses coix seed whisky and a preparation method thereof, and relates to the technical field of fermentation engineering. Comprising the following steps: treating raw materials; mash preparation and saccharification; a fermentation process; a primary distillation process; a secondary distillation process; a barrel aging process; and filling. The core of the method is that an innovative process of partially frying ungerminated coix seed raw materials in combination with synergistic saccharification of exogenous enzyme and malt is adopted, and a brand new process route of'relative low-temperature gelatinization-enzymatic saccharification-non-filtration direct fermentation distillation 'is constructed. Meanwhile, a partial coix seed stir-frying process, malt auxiliary saccharification and Mongolian oak barrel aging combined herbal pretreatment process are further innovatively introduced, and technical support is provided for whisky localization innovation.

The application discloses a construction method and application of Corynebacterium glutamicum for synthesizing shinorine, and belongs to the field of genetic engineering and fermentation technology. The Corynebacterium glutamicum ATCC 13032 is used as a starting strain, a competitive pathway gene is knocked out, an optimal exogenous enzyme is screened, and a key enzyme gene cluster is integrated in multiple copies on a genome, so that the synthesis yield of shinorine in the microbial cell is improved, and the strain can accumulate 0.63 g / L of shinorine in 72 hours in a flask. In fed-batch fermentation in a 50 L fermenter, the final yield of shinorine can reach 4.3 g / L.

This invention discloses a method for the targeted conversion and synthesis of rare ginsenosides Rg3 and Rh2. Specifically, fresh Panax notoginseng leaves are microwave-dried for 1-3 minutes to inactivate their endogenous β-glucosidase. The dried Panax notoginseng leaves are extracted with anhydrous methanol, and the extract is resuspended in an acetate-sodium acetate buffer solution after drying. α-arabinofuranase, β-xylosidase, and β-glucosidase are added to the resuspended solution, and an enzymatic reaction is carried out at 35-40℃ to directionally convert the original ginsenosides into rare ginsenosides Rg3 and Rh2. This method can achieve the inactivation of endogenous enzymes and the stable retention of original saponins in Panax notoginseng leaves in a very short time. Compared with the natural drying combined with exogenous enzymes, the conversion ratios of Rg3 and Rh2 are increased by 3.14 and 2.4 times, respectively. Compared with fresh Panax notoginseng leaves, the contents of Rg3 and Rh2 are increased by 20.6 and 8.7 times, respectively. This method is suitable for the high-value utilization of Panax notoginseng leaf waste resources and the large-scale preparation of rare saponins.

The present invention is a multi-stage treatment that heals tissue or organ damage (e.g., linear defects, fissures, and fibrillations, as well as focal and large defects) in collagen-rich tissues and organs such as articular cartilage. The present invention includes methods 1) to prime tissues in preparation for treatment, which comprises “melting” the tissue matrix, 2) to add or fill the damaged area with a “melding” agent, comprising of endogenous or exogenous tissue matrix, with or without cells, with or without exogenous biomaterials, and with or without endogenous or exogenous enzymes, such that the melding agent enhances anchoring into the defect for the purpose of integration and / or tissue healing. The Melt-and-Meld process can also be applied in conjunction with any existing treatments of tissue or organ defects.

The application discloses a sea cucumber polysaccharide cancer inhibiting preparation and a preparation method and application thereof, and belongs to the technical field of biological medicine. The preparation is prepared from high-purity sea cucumber polysaccharide extracted from Apostichopus japonicus as a core active ingredient, and is matched with a folic acid modified polyethylene glycol-poly lactic acid copolymer targeting carrier and medicinal adjuvants, and is prepared through steps of standardization extraction and purification, targeting carrier preparation, granulation and coating. The application adopts an enzymolysis process combining with self-lyase and exogenous enzyme, and cooperates with multi-step purification and ultrafiltration grading technology to precisely control the purity and molecular weight of the polysaccharide, realizes active targeting of tumor cells through the FA-PEG-PLA targeting carrier, and improves the bioavailability through enteric coating technology. The preparation can up-regulate the expression of TNFR1 protein, activates the caspase cascade reaction to induce tumor cell apoptosis, has a significant inhibiting effect on solid tumors such as lung cancer, kidney cancer and liver cancer, and has very low toxicity to normal cells.

The present invention relates to a process for coloring keratin fibers using a composition C comprising: (i) indican of formula (I) and / or a powder containing it; (ii) henna powder; (iii) at least one exogenous enzyme of the hydrolase type; (iv) at least one exogenous enzyme of the oxidase type; and (v) water.

The invention relates to a preparation method of a yeast extract, and belongs to the technical field of bioengineering. The method comprises the following steps: mixing yeast thalli subjected to fermentation culture, glycine and aspergillus oryzae mycelia subjected to activation treatment, carrying out synergistic autolysis reaction under the mild conditions that the temperature is 45-60 DEG C and the pH value is 4.5-6.5, and carrying out post-enzymolysis refinement by utilizing trace flavourzyme. By constructing the co-autolysis system, efficient biotransformation is realized under mild conditions, and dependence on expensive exogenous enzymes is reduced. The prepared yeast extract is high in content of free amino acids and flavor nucleotides, rich in delicate flavor and harmonious in flavor. The method is simple in process, low in cost and easy to industrialize, and a new scheme is provided for producing high-quality natural seasonings.

This invention discloses a method for preparing lactic acid using a combination of bacterial strains, belonging to the field of microbial fermentation technology. The combination of bacterial strains includes Bacillus subtilis. Bacillus subtilis KC1 and Lactobacillus pentosaccharide Lactiplantibacillus pentosus LB9. The method for preparing lactic acid includes: (1) preparing raw materials and strains, wherein the raw material is baijiu lees; (2) activating Bacillus subtilis and Lactobacillus pentosus separately and mixing them to form a combined strain, and inoculating it into a culture medium with baijiu lees as the sole fermentation substrate and without adding exogenous enzymes or exogenous excipients for fermentation; during the fermentation process, a staged oxygen supply strategy is adopted: first aerobic fermentation, then anaerobic fermentation; (3) collecting lactic acid from the fermentation products. This invention realizes the high-value utilization of lignocellulose waste, effectively coordinates the metabolic conflicts between strains, and solves the problem of efficient conversion of lignocellulose lees into lactic acid.

The invention discloses a poultry meat tenderizing and freshness increasing method based on endogenous enzyme activation, and aims to solve the problems of non-uniform tenderizing and low efficiency due to dependence on exogenous additives in the prior art. The method comprises the following steps: firstly, carrying out pretreatment and stress regulation on poultry meat to obtain raw materials with consistent states; then inducing intracellular energy signal channel activation through intermittent mechanical stimulation and gas environment change; then establishing a microenvironment for activating endogenous calcium-activated protease by using a composite osmotic treatment liquid containing a natural calcium source and assistance of a physical field; then carrying out dynamic standing under the conditions of accurate temperature control, humidity control and specific gas, so as to promote the endogenous protease system to have a sufficient synergistic effect; and finally, carrying out gradient heating and rapid cooling to terminate enzyme activity and lock quality. According to the method, fundamental and uniform tenderization occurring in muscles is achieved, flavor precursor substances are synchronously increased, dependence on exogenous enzyme preparations is not needed, the method conforms to the label cleaning trend, and the processing efficiency is remarkably higher than that of a traditional ripening process.

The present invention relates to a process for coloring keratin fibers using a composition C comprising: (i) indican of formula (I) and / or a powder containing it; (ii) at least one exogenous enzyme of the hydrolase type; (iii) at least one exogenous enzyme of the oxidase type; and (iv) water.

The invention relates to the field of microbial fermentation, in particular to a saccharification and liquefaction bifunctional recombinant yeast strain as well as a construction method and application thereof. The invention provides a recombinant yeast strain with saccharification and liquefaction dual functions, and the recombinant yeast strain has better clinker fermentation and raw material fermentation capabilities through co-expression of high-enzyme-activity alpha-amylase and glucoamylase genes. Under the conditions of clinker fermentation and raw material fermentation, the fermentation alcohol content of existing strains can be improved, addition of exogenous enzymes is reduced, cost reduction and yield increase of biological raw material ethanol can be achieved, and the large-scale industrial production requirement is met.

This invention relates to the field of tea processing technology and discloses a method for increasing the yield of theaflavins using alternating catalysis of exogenous enzymes. The exogenous enzyme is crude pear polyphenol oxidase solution, which is directly prepared from fruit. The method includes the following steps: first, tanninase is added to construct the conversion basis of the crude pear polyphenol oxidase solution; then, the crude pear polyphenol oxidase solution is added, allowing the enzymatic hydrolysis to be completed at room temperature with basic stirring without fermentation, thereby increasing the yield and quality of theaflavins. This invention enables the stepwise and directional conversion of ester-type catechins in tea polyphenol solutions into non-ester-type catechins, laying the foundation for subsequent conversion by exogenous enzymes. The conversion of exogenous enzymes does not require further fermentation and can be carried out at room temperature, resulting in a nearly two-fold increase in theaflavin yield.

This invention provides a method derived from Bacillus ( Bacillus The lactone enzyme AiiA (sp.) GB-1 and its application are described, with its amino acid sequence shown in SEQ ID NO:1. Experimental results show that the enzyme has degradation activity against various N-acyl homoserine lactone signaling molecules, exhibits high degradation ability against long-chain AHLs, and has good stability within a certain temperature and pH range. Further experimental results show that the AiiA enzyme can reduce the quorum sensing-related phenotype of pathogens. Simultaneously, exogenous addition of AiiA enzyme can also reduce the degree of pathogen infection in plant tissues, indicating that the enzyme can be used for plant disease control through engineered bacteria expression or exogenous enzyme preparations. This invention provides a new technical approach for green control of plant diseases.

The invention provides a method for preparing low nucleic acid peptide-enriched yeast protein based on fermentation heat regulation liquid-solid coupling autolysis, which comprises the following steps: liquid high-density fermentation, centrifugation to obtain fresh yeast paste, solid staged stacking autolysis and spray drying. Wherein the solid autolysis adopts a two-stage temperature control strategy: in the earlier stage, a yeast endogenous enzyme system is activated at 30-42 DEG C to efficiently remove nucleic acid and enrich peptide; in the later stage, active temperature control is stopped, the material temperature is naturally increased to 45-60 DEG C by utilizing fermentation heat generated by metabolism of yeast, and cell wall cracking and content release are synergistically promoted. Exogenous enzymes, chemical reagents or instantaneous sterilization are not needed in the whole process, and mixing and temperature regulation are achieved through pile turning. The obtained yeast protein product has the nucleic acid content of less than 1.5 wt%, the peptide content of more than or equal to 70 wt%, low bitter taste, good solubility, energy-saving process and extremely little wastewater, and is suitable for healthy foods and feeds such as plant meat and nutrition bars.

This invention discloses a composite microbial preparation for solid fecal degradation, its preparation method, and its application, belonging to the field of microbial preparation technology. The composite microbial preparation comprises a composite microbial agent and an exogenous composite enzyme preparation; the composite microbial agent includes laccase-producing bacteria, keratinase-producing bacteria, auxiliary degradation bacteria, and composite probiotics; the exogenous composite enzyme preparation includes cellulase, neutral protease, and xylanase. This invention employs a mixed strategy of "exogenous enzyme + enzyme-producing bacteria," rapidly decomposing cellulose, protein, and hemicellulose in the initial stage of composting by adding exogenous cellulase, protease, and xylanase, providing readily available carbon and nitrogen sources for the microorganisms, shortening the composting start-up time to less than 24 hours; simultaneously, the laccase-producing bacteria and keratinase-producing bacteria continuously produce laccase and keratinase during composting, achieving deep degradation of lignin and keratin. This invention has advantages such as rapid start-up, continuous degradation, good deodorization effect, high nitrogen retention rate, and low cost, and has good prospects for industrial application.

The invention belongs to the technical field of biology, and discloses composite fermented rapeseed meal for improving mineral substance utilization of pigs, and the composite fermented rapeseed meal is obtained by fermenting rapeseed meal with composite bacteria consisting of aspergillus oryzae, bacillus subtilis, lactobacillus plantarum and bacteroides cellulolyticus. The compound fermented rapeseed meal adopts four zymophyte, one of the four zymophyte is a unique strain preserved by the applicant, the four zymophyte can achieve efficient synergistic degradation of cellulose and phytic acid, the absorption and utilization efficiency of pigs on mineral substances is remarkably improved, after phytic acid is synergistically degraded by bacteroides cellulolyticus LYH2 and commercial strains, the degradation rate of phytic acid is larger than or equal to 80%, and the degradation rate of phytic acid is larger than or equal to 80%. The cellulose degradation rate is greater than or equal to 65%; when 4-6% of the strain is added into pig daily ration, the utilization rate of minerals can be increased by 31% or above and is increased by 17% or above compared with a DSM 14838 standard strain combination, the use of mineral additives is reduced by 30%, and the excretion of copper and zinc in excrement is reduced by 40% or above. The method does not need an exogenous enzyme preparation, is low in cost and stable in effect, and provides technical support for resource utilization and green cultivation of rapeseed meal. Meanwhile, the invention also provides application of the compound fermented rapeseed meal.