Preparation method of hybrid hydrolase formulation for promoting methane yield from fermentation

Abstract

The method for preparation of hydrolytic enzyme preparation used for improving gas-producing rate of marsh gas fermentation belongs to preparation of supplementary exoenzyme used for marsh gas fermentation. The invention is to provide a method for preparation of hydrolytic enzyme preparation used for improving gas-producing rate of marsh gas fermentation, which has the production bacteria with the following enzymatic vitality index: selecting wheat bran and rice bran as mixed culture medium, obtaining mixed bran seeds by pedigree breed two-stage enlargement culture of the bacteria with solid method, fermenting for 3-4 days at 28 DEG after evenly mixed, sampling and analyzing every enzymatic vitality index, adding commodity enzyme preparation to reach enzymatic vitality index, and airing until the humidity is lower than 5%. The enzymatic vitality index of the prolease in the said mixed bran seeds is not more than 2000+-5% mug tyrosine / g / min. The bacteria can be obtained by bacteria and fungus separated from different environment. The test proves that when hydrolytic enzyme preparation is input into the methane tank fermentation raw materials, the rate of dissolution and degradation of the marsh gas fermentation raw materials can be improve, and the gas-producing rate of marsh gas fermentation, TS and VS gas-production potential and methane content in marsh gas can be increased.

Description

Technical field

[0001] The invention belongs to a preparation method for supplementing exogenous enzymes for biogas fermentation. Background technique

[0002] In 1901, S_hngen pointed out that the process of methane production is a microbial process. In 1967, O.W.Lawrence and P.L.McCarty proposed the stage theory of anaerobic digestion process, namely hydrolysis, hydrogen production, acetic acid production and methane production. Biogas fermentation is a process in which multi-organisms metabolize symbiotically under anaerobic conditions to mineralize organic matter into methane and carbon dioxide. Since the emergence of small-scale high-efficiency biogas digesters in the late 1980s, the popularization and application of biogas has reached a relatively high level. It is very convenient to use and manage. High gas rate, balanced gas production. However, the existing household biogas digesters at home and abroad generally have problems such a...

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