24 results about "MIXED FLORA" patented technology

The invention discloses a method for rapidly enriching pit mud high-yield caproic acid functional flora and application, and belongs to the technical field of baijiu brewing microorganisms. According to the method, the aged pit mud which is screened through tests and has better quality is used as seed mud, and the selected high-quality pit mud is subjected to step-by-step amplification culture by adopting a specific culture medium. The obtained bacterial community is a caproic acid producing bacterial community (85%) with the caproic acid producing clostridium as the main body, and the bacterial community can produce more than 10 g / L of caproic acid and less than 2 g / L of butyric acid. The mixed flora obtained by the method disclosed by the invention can be used for culturing and repairing the pit mud, increasing the caproic acid-producing main flora in the pit mud, promoting the pit mud to be aged, preventing the pit mud from being aged and hardened, and effectively improving the quality of raw wine at the same time. And the mixed flora can also be used for fermenting the byproduct yellow water of the Luzhou-flavor liquor to produce caproic acid at high yield, and can be further used for producing high-quality flavoring liquor.

The invention discloses a domestication method for efficiently degrading tobacco waste to produce hydrogen by using mixed flora, which comprises the following steps: carrying out anaerobic enrichment domestication culture by taking manure compost as a bacterial source and cellulose prepared from tobacco waste as a core component of a domestication culture medium; the domestication culture medium contains cellulose, ammonium bicarbonate and monopotassium phosphate, and the cellulose concentration gradient is gradually increased without adjusting the pH value; stopping culturing when the hydrogen content in the biogas is reduced by 10%, transferring the biogas to a fresh domestication culture medium for passage according to an inoculum size of 10-20%, and continuously domesticating for 20-40 rounds; inoculating the domesticated mixed flora culture solution into a cellulose liquid fermentation culture medium containing microcrystalline cellulose, ammonium bicarbonate, monopotassium phosphate and a nutrient solution according to the inoculum size of 10-20%; the initial pH of the fermentation liquor is adjusted to 6.5-7.5, high-purity nitrogen is introduced to create an anaerobic environment, and hydrogen is produced through fermentation under the conditions of 35 + / -1 DEG C and 120 r / min. According to the method, flora is easy to culture, hydrogen production is efficient and stable, tobacco waste resources can be utilized, flora stability is high, adaptability is high, and strict sterile conditions are not needed.

The invention provides application of an OG7443 gene of trichophyton in identification of trichophyton, a primer pair and an identification method of trichophyton, and relates to the technical field of biology. Based on comparative analysis of genomes of trichophyton and other fungi, the specific marker gene OG7443 which only exists in trichophyton, has single copy and is free of repetitive sequence interference is screened out, and the gene can be used for identification of trichophyton. The primer provided by the invention is designed aiming at the OG7443 gene of trichophyton, has strong specificity and high sensitivity, can accurately distinguish mixed florae possibly existing in a clinical sample, improves the diagnosis accuracy of fungal infection, not only can accelerate the determination of pathogenic bacterium types, but also can improve the diagnosis accuracy of fungal infection. And important technical support can be provided for targeted antifungal treatment and public health prevention and control.

The invention relates to the technical field of bactericides, in particular to an environment-friendly plant-extracted composite sanitizer and application of the environment-friendly plant-extracted composite sanitizer. The traditional Chinese medicine composition comprises the following raw materials: borneol, a pearl derivative, double-petal red rose, ganoderma lucidum, taxus chinensis leaves, a plant source active ingredient extract, sodium salt and purified water. According to the invention, through the synergistic effect of the natural borneol and the pearl derivative, a microbial biofilm barrier is effectively broken through, and efficient and rapid delivery of bactericidal components is realized; intelligent drug loading and directional enrichment characteristics of the nano pearl powder are utilized, and a double-phase protection mechanism combining instant killing and physical blocking is formed; in the whole process, chemical stimulation and environmental pollution are avoided, meanwhile, the broad-spectrum antibacterial advantage of plant active ingredients and the continuous repairing effect of pearl components are perfectly fused, and therefore on the basis of remarkably widening the antibacterial spectrum and improving the sterilization efficiency, multi-target and environment-friendly comprehensive prevention and control over mixed flora infection are achieved.

This invention provides a method for producing single-cell protein using a mixed bacterial culture, comprising the following steps: performing primary seed culture on methanogenic bacteria, *Tricholoma materata*, and *Glucosinolate methyl-eating bacteria* to obtain primary seed solutions; mixing the primary seed culture solutions evenly to obtain a mixed bacterial solution; inoculating the obtained mixed bacterial solution into a secondary seed culture medium for cultivation to obtain a secondary seed solution; inoculating the obtained secondary seed solution into a fermentation medium for scale-up cultivation to obtain a fermentation broth; separating the solid and liquid components of the obtained fermentation broth, harvesting the bacterial cells, and obtaining single-cell protein. This method for producing single-cell protein using a mixed bacterial culture improves the utilization rate of the carbon source methane and the yield of single-cell protein.

The application discloses a mulberry leaf fermentation method based on electric stimulation of bacteria and products and application thereof, and the method comprises the following steps: dissolving dry mulberry leaf powder with water, adding a mixed bacterial population of electrically stimulated Bacillus cereus and Lactobacillus casei plantarum, and performing fermentation culture under the condition of electric stimulation. The fermentation mulberry leaf powder obtained by optimizing different probiotic bacterial species combinations and applying electric stimulation to the fermentation process has not only improved protein content but also greatly reduced anti-nutritional factors phytic acid and tannin, and has obviously improved nutritional value. Compared with other non-fermented mulberry leaves and other bacterial agent fermented mulberry leaves, the fermentation method has obvious advantages, and provides a new method for effective utilization of Chinese silkworm and mulberry resources in China.

The application belongs to the technical field of biological fermentation, and particularly relates to a dark fermentation mixed flora, a culture method thereof and application of the dark fermentation mixed flora in hydrogen production from waste oil reservoirs. In the application, a mixed flora capable of producing hydrogen by using waste oil reservoirs is disclosed, the mixed flora comprising Thermotoga hypogea, Thermotoga elfii, Thermus sp., Propionibacterium freudenreichii, Butyrivibrio fibrisolvens and Pseudomonas aeruginosa. The mixed flora can not only produce hydrogen by using crude oil as raw material, but also improve the flowability of the oil layer, improve the interface between the oil layer and the water layer, and crack heavy components in the crude oil, so that the oil layer can be fully utilized for fermentation hydrogen production, and the efficiency of hydrogen production by microorganisms using waste oil reservoirs is improved.

The present application belongs to the field of traditional Chinese medicine microbial technology, and particularly relates to a method for increasing the content of rapeseed pollen polysaccharide. The method is to increase the content of rapeseed pollen polysaccharide through microbial fermentation, and the microorganism is selected from plant endophytic bacteria and known microbial flora. The microorganism can be a single flora or a mixed flora. The microbial fermentation method can significantly increase the content of plant medicine polysaccharide, create a product probiotic environment, improve the pharmacological activity, and can be widely used in the deep fermentation production of plant medicines.

The invention discloses a method for producing hexanoic acid and octanoic acid by using CO2 biorefinery through mixed flora, and relates to a method for producing hexanoic acid and octanoic acid by using CO2 biorefinery. The problems of low yield and low selectivity of caproic acid and octanoic acid produced by the existing CO2 biorefinery technology are solved. The method comprises the following steps: 1, enriching and domesticating mixed flora; and 2, fermenting the mixed flora. The method is used for producing the hexanoic acid and the octanoic acid through CO2 biorefinery of the mixed flora.

The invention relates to the technical field of detection data analysis, and discloses an electroactive microorganism-based detection data analysis method, which comprises the following steps: constructing an alternate circulation reaction system, inoculating electroactive microorganism mixed flora, recording environmental parameters, calculating time sequence data, and analyzing mixed flora interaction, drug-resistant gene transfer and pellicle characteristics. A four-factor coupling model is constructed, the multi-factor synergistic effect is quantified through index correction and ratio calculation, and the influence of active oxygen generation mechanism fragmentation and catalyst attenuation is solved; a diffusion degradation correlation model is constructed, a comprehensive efficiency index is obtained through analysis, efficiency quantification precision is improved through distribution uniformity correction, and carrier modification and in-situ regeneration double strategies are constructed; a reinforcement learning decision is carried out through carrier modification and in-situ regeneration double strategies, a comprehensive optimization coefficient is calculated to be combined with a near-end strategy optimization algorithm, dynamic decision is carried out by dividing different efficiency levels, metabolism and energy consumption are balanced, operation parameters are flexibly adjusted, and optimization of the overall performance is achieved.

The application discloses a microbial substrate for strawberry planting and a preparation method and application thereof, and the substrate is made of peat, perlite, bacteria-loaded biochar and fungi, and the volume ratio of the peat, the perlite and the bacteria-loaded biochar is (45-75):25:(10-30); the bacteria-loaded biochar is prepared by loading biochar with a biocontrol composite bacteria; and the biocontrol composite bacteria is composed of Rhodopseudomonas palustris, Bacillus subtilis and Bacillus licheniformis. The application changes the traditional single bacterial group into a mixed bacterial group, and uses biochar as a microbial loading material, so that the mixed bacterial group can better improve the prevention and treatment effect, and the biochar can provide a carbon source for the microorganisms and promote the better reproduction and growth of the microorganisms.

A high-density fermentation process using mixed microbial communities to simultaneously achieve rapid biomass growth and high-content PHA synthesis is disclosed. This process uses microbial sludge discharged from the SBR reactor in the PHA-producing bacteria enrichment stage as inoculum and high-COD wastewater as substrate, achieving simultaneous rapid biomass growth and high-content PHA synthesis in a single fermentation. The proposed high-density fermentation process using mixed microbial communities can rapidly proliferate PHA-producing mixed microbial communities within a short time and maintain their high PHA synthesis capacity, resulting in a significant increase in PHA yield. This process can operate stably under high organic loads and can utilize high-concentration organic wastewater, reducing wastewater dilution costs and effectively lowering the cost of PHA production from mixed microbial communities. The proposed high-density fermentation process achieves a PHA volumetric productivity of 4.968 ± 0.048 g / L·d. When integrated into a traditional three-stage PHA synthesis process, it significantly improves the overall PHA yield, ultimately reaching 5.39 g PHA / g seed sludge, which is 8-9 times the yield of traditional batch processes.

The invention provides a method for recovering chromium by reducing chromium-containing tailing leacheate based on microorganisms, hexavalent chromium is reduced into trivalent chromium at normal temperature by using a specific mixed flora, the consumption of chemical agents is remarkably reduced, and green treatment is realized. The reduced trivalent chromium is efficiently adsorbed by cation exchange resin, so that chromium resource recovery is realized, impurity anions such as silicon, aluminum and the like are effectively separated by utilizing cation characteristics of the trivalent chromium, and the recovery purity is further improved by pre-adjusting the pH value. The whole process highly integrates reduction and recovery links, overcomes the problems of long flow and more impurity introduction in the traditional method, can flexibly adjust the strain proportion according to the water quality of the leacheate to optimize the efficiency, and shows the obvious advantages of short flow, low cost and resource utilization.

The invention discloses a functional flora of a tobacco bacterial wilt resistant vaccine and an application of the functional flora. The functional flora of the tobacco bacterial wilt resistant vaccine is a mixed flora of pseudomonas mentaliensis Z3-4 (Pseudomonas mandeli) and pseudomonas silesiensis Z92-2 (Pseudomonas silesiensis), and the functional flora of the tobacco bacterial wilt resistant vaccine is a mixed flora of pseudomonas mentaliensis Z3-4 (Pseudomonas mandeli) and pseudomonas silesiensis Z92-2 (Pseudomonas silesiensis). The two strains are both preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is Beijing, China, and the preservation date is August 18, 2025. According to the invention, a core classification group of tobacco health is determined through field investigation and test for the first time, specific strains are separated and screened from tobacco roots, and a synthetic flora is obtained on the basis of combination of two strains with most remarkable root colonization ability and disease inhibition characteristic, so that tobacco bacterial wilt can be effectively prevented and treated, meanwhile, the growth of tobacco plants is remarkably improved, and the application prospect is wide. Wide application prospects are realized.

This invention relates to a method for biological drying of biogas residue with high moisture content, specifically including the following steps: (1) Inoculating 1-5% of Bacillus thermophilus seed liquid into LB medium and aerobically culturing at 40-60℃ for 24-48h to obtain Bacillus thermophilus bacterial liquid; (2) Adding 0.1-5% of Bacillus thermophilus bacterial liquid to biogas residue with a moisture content of not less than 80%, and mixing evenly to obtain a biological drying substrate; (3) Adding the biological drying substrate prepared in step (2) into a biological drying reactor, and drying at 40-60℃ for 7-15d to obtain organic fertilizer. The method of this invention can be used to treat biogas residue with high moisture content (greater than 80%), without the need to add auxiliary materials to adjust the carbon-nitrogen ratio. By adding microbial agents, the diversity of the mixed bacterial community in the substrate is changed, shortening the treatment time and reducing the energy consumption of the treatment process. The prepared organic fertilizer has low moisture content, high seed germination index, and good decomposition effect.

The invention discloses a method for recycling copper and zinc from high-iron low-grade copper-zinc ore through biological leaching and application, and relates to the technical field of comprehensive utilization of mineral resources. The method for recycling copper and zinc through biological leaching of the high-iron low-grade copper-zinc ore comprises the steps that a mixed flora containing ferrous oxide thiobacillus, thiobacillus thiooxidans, leptospirillum ferriphilum and acidithiobacillus caldus is domesticated, and ore leaching bacterial liquid is obtained; the method comprises the following steps: carrying out biological leaching treatment on a mixture of high-iron low-grade copper-zinc ores by adopting an ore leaching bacterial solution, reducing Fe < 3 + > in a leaching solution into Fe < 2 + >, removing iron in a reducing solution by combining a potential-controlled goethite method, and finally, sequentially recovering copper and zinc. The method can realize efficient recovery of copper and zinc, and has the advantages of environmental protection and low cost.

The invention relates to the technical field of modern breeding industry, and discloses a research method for degrading crude fibers by synergistically fermenting distillers' grains with artificial combined flora, which comprises the following steps: S1, activating single bacteria of the artificial combined flora; s2, preparing an artificial combined flora solution; s3, vinasse pretreatment and screening; s4, preparing a solid fermentation culture medium; s5, inoculation and solid fermentation; s6, monitoring the fermentation process; s7, measuring a product after fermentation; and S8, evaluating the in-vitro digestibility. The three strains are combined in proportion to form an artificial combined flora to cooperatively ferment the vinasse, so that the technical effects of efficiently degrading crude fibers in the vinasse in a targeted manner and cooperatively improving the activity of a key degrading enzyme so as to strengthen the overall degradation efficiency are achieved, and compared with the common technical scheme of single-strain fermentation or simple mixed flora fermentation without a specific proportion in the prior art, the technical scheme has the advantages that the cost is reduced, and the production efficiency is improved. The defects that the crude fiber degradation is not thorough, the enzyme activity secretion type is single, the synergy among strains is poor, and the anti-enzymolysis structure of the crude fiber in the vinasse is difficult to break through are overcome.

The invention provides a method for treating chromite alkaline leaching tailings by microbial mineralization, which comprises the following steps: improving the reduction efficiency of Cr (VI) by utilizing a specific mixed flora through synergistic metabolism, efficiently reducing hexavalent chromium into trivalent chromium under a mild condition, and promoting a reduction product to be further converted into a stable chromium-containing mineral phase by virtue of the induction effect of an iron source and a mineralizing agent, so that the chromite alkaline leaching tailings are subjected to microbial mineralization treatment. The long-term stable curing of Cr is realized, and the problems of Cr (VI) redissolution and secondary pollution which are easy to occur in a traditional chemical detoxification method are fundamentally solved. The technological process is easy to operate and low in energy consumption, high-temperature and high-pressure or complex equipment is not needed, the toxic leaching concentration of the treated chromite alkaline leaching tailings is remarkably lower than the hazardous waste identification standard, enrichment of valuable elements such as chromium and iron in a stable mineral form can be promoted, follow-up resource recycling is facilitated, and good environmental benefits and resource potential are achieved.

The application provides a biological pretreatment method of rare earth ore mining wastewater. The pretreatment method uses a mixed bacterial flora meeting the requirements of bacteria genus obtained through domestication to activate and gradually expand culture; then the mixed bacterial flora is domesticated in different concentrations of wastewater to be treated to adapt to the treatment environment, and finally added into the wastewater to perform deamination treatment. The application uses the mixed bacterial flora to realize rapid denitrification of the rare earth ore mining wastewater through an anaerobic ammonia oxidation process, expands the conventional treatment method, and reduces the load and operation cost of the wastewater treatment system.

The invention discloses a microbial substrate for strawberry planting and a preparation method and application thereof, the substrate is prepared from the following raw materials: peat, perlite, bacterium-loaded charcoal and fungi, and the volume ratio of the peat to the perlite to the bacterium-loaded charcoal is (45-75): 25: (10-30); the bacterium-loaded biochar is prepared by loading biocontrol compound bacteria on biochar; the biocontrol compound bacteria are prepared from rhodopseudomonas palustris, bacillus subtilis and lysine bacillus. The traditional single flora is changed into the mixed flora, the biochar is used as a microorganism loading material, the mixed flora can better improve the prevention and treatment effect, and the biochar can also provide a carbon source for microorganisms and promote the microorganisms to better breed and grow.

The invention relates to the technical field of underground water pollution low-carbon treatment, in particular to a method for synchronously removing benzene series and hexavalent chromium in acidic underground water, which comprises the following steps: gradually replacing electron acceptors required by microorganisms, obtaining acid-resistant anaerobic mixed flora capable of degrading the benzene series and simultaneously generating iron minerals from soil, the method comprises the following steps: adding ferric citrate and an acid-resistant anaerobic mixed flora into acidic polluted underground water containing benzene series and hexavalent chromium, culturing, degrading the benzene series, reducing the hexavalent chromium, and fixing part of the hexavalent chromium in iron minerals. According to the method, anaerobic microorganisms are used for degrading benzene series and generating iron minerals under the acidic condition, and in the process, hexavalent chromium is reduced and fixed by the iron minerals at the same time. Compared with the prior art, the method is suitable for acidic polluted underground water, does not need additional pH regulation and control, does not need oxygen supply, is simple to operate, can synchronously repair combined pollution, and is environment-friendly and free of secondary pollution.

The invention discloses a method for promoting mixed flora to produce PHA (polyhydroxyalkanoate), which comprises the following steps: carrying out enrichment culture on aerobic granular sludge under electrical stimulation, placing the obtained mixed flora in a Fed-Batch reactor, carrying out synthetic culture under electrical stimulation, and when the pH value in the reactor is increased to 9.5 + / -0.5 and is kept unchanged, settling, discharging part of supernate and supplementing materials, and when the pH value in the reactor after material supplementing is less than 9 and is kept unchanged, culture is stopped, and the mixed flora accumulated with PHA is obtained. According to the method disclosed by the invention, a process of combining electrical stimulation, real-time measurement of the pH value in a PHA production stage and timely regulation and control of a synthetic culture process according to the change condition of the pH value is adopted, so that the mixed flora can be promoted to produce the PHA under a low-cost condition, and the accumulation amount of the PHA in the mixed flora can be remarkably increased; and in addition, the mixed flora containing the maximum PHA accumulation amount can be separated from the system in time, and secondary pollution cannot be caused.

The application discloses a microbial agent for promoting growth of cucumbers, a preparation method and application thereof, and belongs to the technical field of agricultural microorganisms. The microbial agent comprises the following components: denitrifying achromobacter, Bradyrhizobium elkanii and Azotobacter vinelandii, and the volume ratio of the three is 2:1:1. The bacteria with growth promotion are reasonably combined and used, so that the mixed bacterial flora of the application is superior to a single strain in terms of growth promotion effect on cucumbers, and the synergistic effect among the bacterial strains is more adaptable in the actual soil rhizosphere complex environment. Through determination of the number of bacterial strains and mixing of specific proportions, the growth of cucumber seeds is stably and effectively promoted, the root elongation is promoted, the flowering and fruit setting rate is improved, and the plant height, plant biomass and chlorophyll content of cucumber seedlings are improved. In addition, the microbial agent can help improve the growth quality and yield of plants, reduce environmental pollution, has wide application potential in the fields of agriculture and ecology, and is worth promoting.

The invention belongs to the field of concrete materials, and discloses a preparation method and application of microbial self-repairing concrete based on a pelelith carrier. The self-repairing concrete is prepared from the following raw materials in parts by weight: 5 to 10 parts of bacillus pasteurii bacterial liquid, 964 to 1128 parts of water, 578 to 677 parts of cement, 1445 to 1693 parts of fine aggregate, 2023 to 2370 parts of coarse aggregate, 3 to 4 parts of water reducing agent, 5 to 10 parts of urea, 5 to 10 parts of calcium acetate, 4 to 8 parts of pelelith and 15 parts of covering material. The vesuvianite is used as a carrier, the mixed flora and the required nutrient substances are wrapped by the corresponding covering material, when the concrete cracks, the surface wrapping cracks, the flora and the nutrient substances in the concrete can be released, the nutrient substances provide nutrients for strains to rapidly form spores, and meanwhile, the spores are prevented from being damaged. And more free calcium ions are provided for a flora mineralization deposition reaction, so that formation of spores and calcium carbonate can be induced.