30 results about "Acidophilic bacteria" patented technology

The invention discloses a method for leaching waste lithium battery positive electrode powder by using intestinal flora in a neutral environment, and belongs to the technical field of resource recycling, the method comprises the following steps: step 1, a pretreatment link, the pretreatment link comprises the following process links, and each link has no sequence: (1) preparation of battery powder, (2) acquisition of intestinal flora, and (3) preparation of a culture medium; 2, domesticating the flora; and 3, valuable metal in the waste battery powder is leached out. According to the method, the intestinal flora is applied to the biological leaching of the waste LIBs positive electrode powder, so that the dependence of traditional strong acidophilic bacteria on an extremely low pH condition is broken through, and the environmental harm is greatly reduced; according to the method, inorganic acid does not need to be added, the pH value of a reaction system is stabilized within the nearly neutral range of 7.8-8.2, equipment loss caused by acid etching is reduced, reagent consumption for subsequent metal recovery and neutralization treatment is reduced, and remarkable economic benefits and environmental benefits are achieved.

This invention discloses the preparation and application of self-modified iron-based materials for removing heavy metal and organic pollutants. Acidophilic bacteria with stable iron-oxidizing capabilities are cultured in a medium and collected by centrifugation to obtain a large number of bacterial cells. These cells are then added to a culture medium to synthesize iron-containing secondary minerals, which are then pyrolyzed to form modified iron-based materials. Alternatively, the bacterial cells are added to iron-containing culture media with different concentrations of aluminum ions to synthesize aluminum ion-modified iron-based biomaterials of different morphologies and sizes. This product is synthesized by bacteria at room temperature, with the self-modification process of the iron-based material driven under pyrolysis conditions or an aluminum ion environment. It is environmentally friendly and allows for controllable synthesis. It is suitable for treating wastewater containing different types and concentrations of heavy metal and organic pollutants, with high treatment efficiency. Cr(VI) removal rate reaches over 90%, and organic pollutant removal rate reaches 100%. The material is recyclable and has broad application prospects.

The invention relates to a metal leaching agent and a method for leaching and recovering metal from a retired photovoltaic module step by step. The metal leaching agent comprises an acidophilic thiobacillus ferrooxidans solution, a hidden acidophilic bacteria solution and a thiobacillus thiooxidans solution, the volume ratio of the acidophilic thiobacillus ferrooxidans liquid to the hidden acidophilic bacteria liquid to the thiobacillus thiooxidans liquid is (0.5-1.5): (0.5-1.5): (0.5-1.5); wherein the concentration of the acidophilic thiobacillus ferrooxidans, the acidophilic hidden bacteria and the thiobacillus thiooxidans is (1-9) * 10 < 8 > cells / mL. Furthermore, the invention provides a method for leaching and recovering metal from the retired photovoltaic module step by step. According to the method, the metal leaching agent is utilized, mild and green recovery of aluminum and silver in the decommissioned photovoltaic cell piece and full-process recovery of the decommissioned photovoltaic module can be achieved through step-by-step biological leaching, and the method has the excellent leaching effect on aluminum and silver.

The application provides a recovery method of nickel, cobalt, manganese and lithium. The recovery method comprises the following steps: step S1, sequentially disassembling, crushing, heat treating and removing magnetism of lithium battery recovery material to obtain black powder; step S2, decomposing the black powder by using acidophilic bacteria solution to remove copper elements and aluminum elements, and obtaining a decomposed product; step S3, acid leaching the decomposed product to obtain a leaching solution; step S4, fractionally extracting the leaching solution to obtain a raffinate, a manganese salt solution, a cobalt salt solution and a nickel salt solution; step S5, evaporating and crystallizing the raffinate to obtain a lithium salt solution; and step S6, independently co-precipitating the manganese salt solution, the cobalt salt solution, the nickel salt solution and the lithium salt solution to obtain manganese salt solid, cobalt salt solid, nickel salt solid and lithium salt solid. The recovery method provided by the application is helpful to improve the purity and yield of the recovery of nickel, cobalt, manganese and lithium.

The present disclosure relates to methods, compositions, and kits for determining whether a liquid contains a guaiacol-producing bacterium. In embodiments, a method comprises the steps of (a) mixing a media that changes color with a change in pH with a volume of a liquid that is potentially contaminated with one or more guaiacol-producing bacteria to create a sample, (b) placing the sample in a sterile container, (c) incubating the sample in the sterile container at about 30°C to about 60°C, (d) determining whether the sample has undergone a color change, an odor change, and / or a change in clarity following step (c), and (e) determining whether the liquid contains a guaiacol-producing bacteria based upon the determination in step (d). In embodiments, the liquid that is potentially contaminated with one or more guaiacol-producing bacteria is an acidic fruit juice. In embodiments, the guaiacol-producing bacteria are guaiacol-positive thermophilic acidophilic bacteria.

The present invention relates to a leaching method for leaching mixed powder of electronic waste using Lactobacillus plantarum, and belongs to the field of resource recovery technology. The leaching method described in the present invention comprises the following steps: preparing mixed powder of electronic waste, configuring culture medium, acclimating bacteria, and leaching. The present invention applies Lactobacillus plantarum to the biological leaching of a mixed material of waste lithium iron phosphate batteries and waste printed circuit boards, thereby achieving the extraction of lithium and iron from waste lithium iron phosphate batteries and the extraction of copper from waste printed circuit boards. Compared with traditional biological leaching of single electronic waste, the present method does not use strong acidophilic bacteria, does not produce strongly acidic wastewater, and does not add additional chemical reagents, which greatly reduces the water and soil acidification problem caused by traditional strong acidophilic bacteria, and can achieve efficient biological leaching of a mixed material of waste lithium iron phosphate batteries and waste printed circuit boards without producing a large amount of strong acid that is harmful to the environment.

The present invention discloses C 14 The invention discloses a method for regulating acidophilic bacteria leaching, which belongs to the field of biological leaching technology. The invention finds that different types of AHL signal molecules can have different effects on the growth and leaching of acidophilic bacteria at different concentrations and different addition times. The signal molecule control experiment confirms that C 14 At certain concentrations, the HSL signal molecule can promote and enhance the production of extracellular polysaccharides and fulvic acid by the strain, as well as its ability to adhere to minerals in the later stages of leaching, thereby increasing the strain's growth rate and mineral leaching efficiency. By regulating the concentration of the signal molecule, the rate of acidophilic leaching and its growth can be adjusted. This method is simple to operate, has low production costs, and is environmentally friendly. This method solves the problem of difficult control during mixed acidophilic leaching, promotes the industrial development of biometallurgical technology, and improves leaching production efficiency.

The application discloses a method for leaching waste lithium battery positive pole powder in a neutral environment by using common desulfurization vibrio, and belongs to the field of waste battery metal resource recycling and bio-metallurgical technology. The common desulfurization vibrio obtained through gradient domestication is inoculated into a modified culture medium, and then the bacterial liquid is activated to construct a bioleaching system, so that the valuable metals in waste NCM, NCA, LFP, LCO, LMO and other positive pole materials are bioleached. The common desulfurization vibrio is innovatively used as the leaching strain, and the dependence on acidophilic bacteria in traditional bioleaching is abandoned, so that the efficient dissolution and directional separation of valuable metals in various waste LIBs positive pole materials are realized under mild neutral conditions, and a new microbial technical scheme is provided for waste LIBs positive pole valuable metal recovery.

The application discloses application of a microbial composition in industrial hazardous waste treatment and belongs to the technical field of industrial hazardous waste treatment and comprises the following steps: S1, collecting industrial hazardous waste, obtaining hazardous waste materials after pretreatment of the industrial hazardous waste, and filling the hazardous waste materials in a fermentation tank; S2, adding a growth medium in a bioreactor, connecting domesticated bacterial liquid to the bioreactor for expansion culture, and obtaining fermentation materials of compound acidophilic bacteria; S3, adding a biosurfactant to the fermentation materials of the compound acidophilic bacteria, uniformly stirring, and obtaining cell membrane surface hydrophobic modified compound acidophilic bacteria; and S4, then adding an acidic pH buffer solution to obtain a to-be-treated material. The application can maintain the stability of the pH required by the acidophilic bacteria in the process of treating the industrial hazardous waste and improve the cell membrane surface hydrophobicity of the compound acidophilic bacteria.

The present invention discloses a method for improving the acid tolerance of E. coli by integrating the molecular chaperone CbpA of extreme acidophilic bacteria, belonging to the fields of biochemistry and molecular biology. The present invention provides a molecular chaperone protein CbpA from Acidithiobacillus thermophilum acidophilus and its encoding gene; the molecular chaperone protein CbpA can improve the acid stress resistance of Escherichia coli, and through gene editing technology, the genome of E. coli BL21 is modified to obtain recombinant Escherichia coli. By detecting the growth status and organic acid tolerance of wild-type BL21, defective BL21-ΔcbpA, and recombinant strain BL21-ΔcbpA / AccbpA strains under acidic conditions, its acid tolerance was confirmed. The present invention lays a foundation for later improving the acid tolerance performance of engineering strains through macromolecular repair means such as molecular chaperone proteins, so as to improve the robustness of industrial microorganisms.

The invention belongs to the technical field of biology, and particularly relates to a method for improving acid-eating bacterium tolerance and degrading high-concentration pyridine. According to the specific technical scheme, the method for improving the tolerance and pyridine degradation capacity of the acidovorax sp. Comprises the following steps: extracting a corncob medullary layer leaching solution, and adding the corncob medullary layer leaching solution into a growth environment of the acidovorax sp. According to the invention, it is found for the first time that the corncob medullary layer leachate can effectively improve the tolerance and high-concentration pyridine degradation capability of acidovorax. By adding the corncob medullary layer leachate in the culture environment of the acidovorax bacteria, the acidovorax bacteria can degrade 99% or more of about 2700 mg / L of pyridine after being treated for about 84 hours. The method provided by the invention can effectively improve the high-concentration pyridine degradation effect of the acidovorax LD-B, the method is simple, and the used corncob is an agricultural waste, is wide in source and low in price, has both environmental friendliness and sustainability, greatly reduces the application cost, and has huge popularization value.

This application provides a solid culture medium for the isolation and purification of thermophilic acidophilic bacteria, its preparation method, and its application. The solid culture medium is prepared by mixing a nutrient composition, a coagulant, and deionized water. The coagulant accounts for 9% to 50% of the solid culture medium by mass. The coagulant is a high-acyl gellan gum, containing 3 to 4 acyl groups. The concentration of the high-acyl gellan gum is 5 g / L to 30 g / L. This solid culture medium is made using high-acyl gellan gum. By adjusting the mass percentage of the high-acyl gellan gum, especially by limiting its concentration, the solid culture medium can remain stable for a long time under high temperature and high acid conditions, maintaining good gelation properties. This provides a stable high-temperature and high-acid growth environment for thermophilic acidophilic bacteria, thus facilitating the successful isolation and purification of these bacteria. Furthermore, high-acyl gellan gum is widely available and inexpensive, further reducing culture costs.

The invention belongs to the field of solid waste treatment, and discloses a Fenton iron mud resource utilization method and system. The method comprises the following steps: feeding the Fenton iron sludge into the first bioreactor; under the anaerobic condition, the dissimilatory iron reducing bacteria produce electrons through metabolism of a carbon source, ferric iron is reduced into ferrous iron, meanwhile, the carbon source is subjected to hydrolysis and acidification, small-molecule organic acid, CO2 and H2 are generated, supernate containing ionic state Fe < 2 + > and the small-molecule organic acid is obtained and fed into a second bioreactor, an acid environment and a small-molecule carbon source are provided for iron reducing acidophilic bacteria, and the iron reducing acidophilic bacteria are obtained. Ferric iron is reduced into ferrous iron, and supernate containing ionic state Fe < 2 + > is obtained; and carrying out solid-liquid separation on the supernatant containing the ionic state Fe < 2 + > to obtain an acidic filtrate, and sending the acidic filtrate back to the Fenton reactor as a ferrous iron source. According to the method, excessive COD is not generated and flows back to the Fenton reactor, and Fe < 2 + > ion state recovery is realized without additionally adding acid.

The invention is applicable to the technical field of agricultural disease prevention and control, and provides a method for efficiently and nondestructively treating seeds carrying bacterial fruit blotch pathogenic bacteria and application, the method comprises the following steps: S1, treating the seeds carrying diseases by using low-temperature plasma; s2, performing molecular biological detection on the treated seeds; s3, sowing the treated seeds into a seedling raising tray for seedling raising; a dielectric barrier discharge (DBD) device is adopted in the low-temperature plasma treatment in the step S1. The low-temperature plasma treatment method has an obvious killing effect on watermelon acidophilic bacteria inside and outside the seeds, the seed treatment efficiency is improved, the purpose of preventing and controlling the melon bacterial fruit blotch is achieved, the risk of the diseases is effectively avoided, and huge economic losses caused by the fruit blotch of farmers and enterprises are reduced; the seed vigor can be enhanced, the bud vigor and bud rate of the watermelon seeds can be remarkably improved, the germination and growth period of the seeds can be shortened, the seedling emergence uniformity of the seeds can be improved, and the seedling commodity can be improved.

The present disclosure provides compositions, methods, and kits for detecting guaiacol-producing microorganisms, in particular, Thermophilic Acidophilic Bacteria (TAB) in foodstuff. In one example, a method comprises: (a) contacting a set of oligonucleotides with the sample, wherein the set of oligonucleotides comprising: a first collection of primers targeted for nucleic acids of a guaiacol-producing microorganism; and a second collection of primers targeted for nucleic acids of Alicyclobacillus acidoterrestris; (b) amplifying DNA in the sample with the said set of oligonucleotides under a multiplex polymerase chain reaction (PCR); and (c) determining the presence of PCR products of step (b), wherein the presence of a PCR product in the sample is indicative of contamination of the sample by both guaiacol-producing microorganism and Alicyclobacillus acidoterrestris.

The present invention belongs to the field of fertilizers, particularly organic fertilizers, and to the field of processes for preparation of fertilizers, which comprise biological or biochemical steps, such as composting or fermentation. The invention relates to a process and a system for continuous production of organic fertilizer and organic fertilizer produced with said process. The process comprises preparing or providing a fermentation agent, which comprises inoculation of fruit pomace with a microbial community comprising at least fungi, actinomycetes, thermophilic microorganisms and acidophilic bacteria, and preparing a fertilizer by mixing the fermentation agent and the raw material. In the process, acidophilic bacteria are sequentially activated, which break down fats and proteins in the input raw material, then the decomposition of cellulose and the activation of thermophilic microorganisms occur due to the increase in temperature. This is followed by the mineralization and formation of humates from the humic acids formed in the previous steps, the optional addition of nitrogen, phosphorus, and / or potassium to form organic-mineral complexes, and a drop in temperature to room temperature or ambient temperature, which is a sign that the fertilizer preparation process is finished. Raw material is added at the first end of the fermentation shaft, and with mixing the entire mass moves due to mixing towards the second end of the fermentation shaft, where the final fertilizer with humates is present, while at the first end fresh raw materials and fermentation agent can be added.

The invention discloses sulfur disproportionated bacteria and application thereof in phosphorus recovery of anaerobic digestion sludge, and belongs to the field of sludge recycling. The sulfur disproportionated bacterium is sulfur-vibrio nanjinggense JS02 screened by the inventor from anaerobic digestion sludge, the bacterium is identified as a novel sulfur-vibrio species through molecules, and HS-generated through biological sulfur disproportionation reaction rapidly replaces PO4 < 3-> combined with metal in the sludge and releases the PO4 < 3-> into a liquid phase under the conditions that a small amount of sulfur source is externally added and the pH is neutral and slightly alkaline, the reaction condition is mild, the operation is simple, the cost is low, and the sulfur disproportionated bacterium is suitable for large-scale industrial production. The release and dissolution of phosphorus in anaerobic digestion sludge can be realized without acidizing and cracking sludge cells, and meanwhile, the release of metal cations in a liquid phase can be greatly reduced. Compared with phosphorus in the sludge acidified and leached through acidophilic bacteria, the method has the advantages that a large amount of acid waste liquid and acid mud cakes are avoided, the procedure of removing impurities from metal ions can be omitted for crystallization recovery of PO4 < 3-> in a subsequent liquid phase, and the whole recovery process is more environmentally friendly and efficient.

The invention discloses a method for leaching anode powder of a waste lithium battery in a neutral environment by utilizing common desulfurization vibrio, and belongs to the technical field of waste battery metal resource recovery treatment and biological metallurgy. The method comprises the following steps: inoculating common desulfurization vibrio obtained through gradient domestication into an improved culture medium, then activating a bacterial liquid, and constructing a biological leaching system, so that the biological leaching of valuable metals in waste NCM, NCA, LFP, LCO, LMO and other positive electrode materials is realized. According to the method, common desulfurization vibrio is creatively adopted as a leaching strain, the dependence of traditional biological leaching on acidophilic bacteria is abandoned, efficient dissolution and directional separation of valuable metals in various waste LIBs positive electrode materials are achieved under the mild neutral condition, and a brand-new microbial technical scheme is provided for waste LIBs positive electrode valuable metal recovery.

The invention relates to the technical field of agriculture, and discloses a microorganism-mineral based composite material as well as a preparation method and application thereof. The microorganism-mineral-based composite material comprises the following components: iron phosphate powder and dissimilatory iron reduction microorganisms, wherein the dissimilatory iron reducing microorganisms comprise at least one of shewanella, geobacter, desulfurization vibrio, clostridium butyricum, geoacidophilus and iron-rich bacteria. The microorganism-mineral-based composite material provided by the invention comprises the mineral component iron phosphate and dissimilatory iron reduction microorganisms, and the dissimilatory iron reduction microorganisms can actively reduce the iron phosphate which is originally stable in chemical property and insoluble into Fe (II) with relatively high solubility, so that the crystal structure of the iron phosphate is destroyed; the phosphate fertilizer can release free PO4 < 3-> which can be absorbed and utilized by plants, the effect of the mineral reduction dissolution process driven by microorganisms is far better than that of simple addition when minerals or microorganisms are independently used, and an efficient phosphorus activation mechanism is formed.

A mine leaching pollution buffer zone and its application belong to the field of mine pollution prevention and control technology, and overcome the defects of the existing technology that it is not applicable to mines under mining, steep slopes and the like, and cannot prevent the continuous pollution of surrounding soil and water bodies during the mining process or the accumulation of leaching water. The mine leaching pollution buffer zone of the present invention includes a water storage zone, a deceleration pretreatment zone, a non-metallic adsorption precipitation zone and a heavy metal ion enhanced adsorption zone arranged in sequence. The present invention adopts a multi-layer pollution blocking zone construction method, which can achieve simultaneous blocking of metal pollutants and non-metallic pollutants, while improving the pH of the leaching water, killing acidophilic bacteria, filtering divalent iron ions, and avoiding the terminal regeneration of acidic leaching water.

The invention provides a high-degradation microbial deodorization and degradation bacterial agent, and relates to the technical field of microbial degradation. The invention relates to a high-degradation microbial deodorization degradation bacterial agent which comprises microbial strains, a carbon source, a pH regulator, a surfactant, titanate modified hydrogen peroxide and nano titanium dioxide, and the microbial strains comprise nitrifying bacteria, denitrifying bacteria, acidophilic bacteria and alkaliphilic bacteria. The compound microbial deodorization and degradation microbial agent can reduce the pollution of peculiar smell substances and organic matters in wastewater / garbage. The microbial strains enhance the conversion and degradation process of peculiar smell substances through mutual synergistic effect, and substances for promoting oxidation reaction are added, so that the oxygenolysis efficiency is improved, and the deodorization effect is improved.

The application discloses a method for leaching positive electrode powder of waste lithium battery in a neutral environment by using intestinal flora, and belongs to the technical field of resource recovery, and comprises the following steps: step 1: a pretreatment link, the pretreatment link comprises the following process links, and the links have no sequence: (1) preparation of battery powder, (2) acquisition of intestinal flora, and (3) preparation of culture medium; step 2: domestication of the flora; and step 3: leaching of valuable metals in the waste battery powder. The intestinal flora is applied to biological leaching of the positive electrode powder of the waste LIBs, breaks through the dependence of traditional strong acidophilic bacteria on extremely low pH conditions, and greatly reduces environmental hazards. In the application, inorganic acid does not need to be added, the pH of a reaction system is stably in a near-neutral range of 7.8-8.2, equipment loss caused by acid etching is reduced, reagent consumption in subsequent metal recovery and neutralization treatment is reduced, and remarkable economic benefits and environmental benefits are achieved.

The invention relates to a method for leaching electronic waste mixed powder by using lactobacillus plantarum, and belongs to the technical field of resource recovery. The leaching method comprises the following steps: preparing electronic waste mixed powder, preparing a culture medium, domesticating bacteria and leaching. According to the method, lactobacillus plantarum is applied to biological leaching of the mixed material of the waste lithium iron phosphate battery and the waste printed circuit board, and extraction of lithium and iron in the waste lithium iron phosphate battery and extraction of copper in the waste printed circuit board are achieved at the same time. Compared with traditional biological leaching of single electronic waste, the method has the advantages that strong acidophilic bacteria are not used, strong acid wastewater is not generated, additional chemical reagents are not added, the problem of water and soil acidification caused by the traditional strong acidophilic bacteria is greatly relieved, and the method can be used for recycling the electronic waste under the condition that a large amount of strong acid harmful to the environment is not generated. And efficient biological leaching of the mixed material of the waste lithium iron phosphate battery and the waste printed circuit board is realized.

This invention provides a heat-resistant acidophilic bacteria proliferation inhibitor and a method for inhibiting heat-resistant acidophilic bacteria proliferation in drinks. This invention relates to, e.g., a heat-resistant acidophilic bacteria proliferation inhibitor containing an active ingredient constituted by a 3-hydroxybutyric acid or a salt thereof.

The invention relates to the technical field of descaling, and discloses a thermophilic acid-producing bacterium geothermal well anti-scaling and descaling composite agent and a preparation method thereof, the thermophilic acid-producing bacterium geothermal well anti-scaling and descaling composite agent comprises the following components: a thermophilic acid-producing bacterium agent, maleic anhydride grafted modified polyaspartic acid, organic phosphonate, a silicon dispersant, a corrosion inhibitor, a stabilizer, a temperature adaptive composite additive and deionized water; the composite agent disclosed by the invention not only has excellent comprehensive performance, but also has the advantages of environmental protection, practicability, long service life and the like, and the overall technical scheme is scientific and reasonable and high in practicability. Compared with an existing chemical agent, the microbial components are introduced, the dosage of the chemical agent is reduced, the environment-friendly effect is achieved, microbial metabolites can be naturally degraded, and secondary pollution cannot be caused; compared with an existing microbial agent, through component synergy, the problem of high-temperature inactivation of strains is solved, and the long-term effectiveness of the agent is improved.

The application of vanillin in promoting the leaching efficiency of acidophilic bacteria is disclosed. Through the experiments of different concentrations of vanillin on the physicochemical parameters of bioleaching, mineral adhesion and EPS production of acidophilic bacteria, it is proved that the addition of 10 mg / L vanillin is beneficial to the leaching of minerals; the addition of 50 mg / L vanillin promotes the mineral leaching and the growth of strains in long-term leaching (>9 days). This strategy of improving the iron and sulfur oxidation ability of acidophilic bacteria through a new type of signal molecule lays a theoretical foundation for improving the leaching efficiency of strains in the bioleaching industry, provides a new idea for solving the environmental pollution problems such as acid mine drainage, and has a broad development prospect.

This invention provides in one aspect compositions that improve the corrosion-resistance of cementitious materials. In certain embodiments, the compositions of the invention inhibit the growth of acidophilic bacteria thriving in / on cementitious material.

The application discloses acidified seed sludge and a preparation method thereof, and belongs to the technical field of sludge treatment. The method is to add glycine to sludge after heating the sludge to 55 DEG C, and then to carry out anaerobic fermentation treatment to obtain acidified seed sludge. The acidified seed sludge is rich in acidophilic bacteria Clostridia, and the relative abundance reaches 15-35%. The acidified seed sludge has good acid resistance and high acid production rate. When the seed sludge is inoculated into a new sludge environment, the seed sludge can rapidly react, rapidly consume extracellular polymeric substances (EPS) and polysaccharide substances in cell walls in the sludge system, reduce the content of sludge organic matters and the viscosity, and achieve the effect of rapid acid production.

The invention discloses a method and beverage for inhibiting propagation of actinobacillus acidotartrate by using inactivated lactic acid bacteria, and belongs to the technical field of food bacteriostasis. The preparation method comprises the following steps: inactivating lactic acid bacteria with activity to obtain inactivated lactic acid bacteria; cracking the inactivated lactic acid bacteria to obtain inactivated lactic acid bacteria lysate; the inactivated lactic acid bacteria lysate is added into a food system needing to inhibit the propagation of the acidobacillus acidotartrate, and the inactivated lactic acid bacteria lysate can significantly inhibit the growth and propagation of stubborn thermoacidophilic bacteria represented by the acidobacillus acidotartrate in the food system under mild conditions (without high temperature or chemical preservatives). The method disclosed by the invention not only avoids the damage of traditional high-temperature sterilization to the flavor and nutrition of the product, but also conforms to the development trend of'clean labels' and no chemical additives in the food industry, and provides a new solution for controlling microbial spoilage.

The present disclosure provides compositions, methods, and kits for detecting guaiacol-producing microorganisms, in particular, Thermophilic Acidophilic Bacteria (TAB) in foodstuff. In one example, a method comprises: (a) contacting a set of oligonucleotides with the sample, wherein the set of oligonucleotides comprising: a first collection of primers targeted for nucleic acids of a guaiacol-producing microorganism; and a second collection of primers targeted for nucleic acids of Alicyclobacillus acidolerreslris: (b) amplifying DNA in the sample with the said set of oligonucleotides under a multiplex polymerase chain reaction (PCR); and (c) determining the presence of PCR products of step (b), wherein the presence of a PCR product in the sample is indicative of contamination of the sample by both guaiacol- producing microorganism and Alicyclobacillus acidoterrestris.