31 results about "Acidophilic bacteria" patented technology

The invention discloses a method for leaching waste lithium battery positive electrode powder by using intestinal flora in a neutral environment, and belongs to the technical field of resource recycling, the method comprises the following steps: step 1, a pretreatment link, the pretreatment link comprises the following process links, and each link has no sequence: (1) preparation of battery powder, (2) acquisition of intestinal flora, and (3) preparation of a culture medium; 2, domesticating the flora; and 3, valuable metal in the waste battery powder is leached out. According to the method, the intestinal flora is applied to the biological leaching of the waste LIBs positive electrode powder, so that the dependence of traditional strong acidophilic bacteria on an extremely low pH condition is broken through, and the environmental harm is greatly reduced; according to the method, inorganic acid does not need to be added, the pH value of a reaction system is stabilized within the nearly neutral range of 7.8-8.2, equipment loss caused by acid etching is reduced, reagent consumption for subsequent metal recovery and neutralization treatment is reduced, and remarkable economic benefits and environmental benefits are achieved.

This invention discloses the preparation and application of self-modified iron-based materials for removing heavy metal and organic pollutants. Acidophilic bacteria with stable iron-oxidizing capabilities are cultured in a medium and collected by centrifugation to obtain a large number of bacterial cells. These cells are then added to a culture medium to synthesize iron-containing secondary minerals, which are then pyrolyzed to form modified iron-based materials. Alternatively, the bacterial cells are added to iron-containing culture media with different concentrations of aluminum ions to synthesize aluminum ion-modified iron-based biomaterials of different morphologies and sizes. This product is synthesized by bacteria at room temperature, with the self-modification process of the iron-based material driven under pyrolysis conditions or an aluminum ion environment. It is environmentally friendly and allows for controllable synthesis. It is suitable for treating wastewater containing different types and concentrations of heavy metal and organic pollutants, with high treatment efficiency. Cr(VI) removal rate reaches over 90%, and organic pollutant removal rate reaches 100%. The material is recyclable and has broad application prospects.

The invention relates to a metal leaching agent and a method for leaching and recovering metal from a retired photovoltaic module step by step. The metal leaching agent comprises an acidophilic thiobacillus ferrooxidans solution, a hidden acidophilic bacteria solution and a thiobacillus thiooxidans solution, the volume ratio of the acidophilic thiobacillus ferrooxidans liquid to the hidden acidophilic bacteria liquid to the thiobacillus thiooxidans liquid is (0.5-1.5): (0.5-1.5): (0.5-1.5); wherein the concentration of the acidophilic thiobacillus ferrooxidans, the acidophilic hidden bacteria and the thiobacillus thiooxidans is (1-9) * 10 < 8 > cells / mL. Furthermore, the invention provides a method for leaching and recovering metal from the retired photovoltaic module step by step. According to the method, the metal leaching agent is utilized, mild and green recovery of aluminum and silver in the decommissioned photovoltaic cell piece and full-process recovery of the decommissioned photovoltaic module can be achieved through step-by-step biological leaching, and the method has the excellent leaching effect on aluminum and silver.

The application provides a recovery method of nickel, cobalt, manganese and lithium. The recovery method comprises the following steps: step S1, sequentially disassembling, crushing, heat treating and removing magnetism of lithium battery recovery material to obtain black powder; step S2, decomposing the black powder by using acidophilic bacteria solution to remove copper elements and aluminum elements, and obtaining a decomposed product; step S3, acid leaching the decomposed product to obtain a leaching solution; step S4, fractionally extracting the leaching solution to obtain a raffinate, a manganese salt solution, a cobalt salt solution and a nickel salt solution; step S5, evaporating and crystallizing the raffinate to obtain a lithium salt solution; and step S6, independently co-precipitating the manganese salt solution, the cobalt salt solution, the nickel salt solution and the lithium salt solution to obtain manganese salt solid, cobalt salt solid, nickel salt solid and lithium salt solid. The recovery method provided by the application is helpful to improve the purity and yield of the recovery of nickel, cobalt, manganese and lithium.

The present disclosure relates to methods, compositions, and kits for determining whether a liquid contains a guaiacol-producing bacterium. In embodiments, a method comprises the steps of (a) mixing a media that changes color with a change in pH with a volume of a liquid that is potentially contaminated with one or more guaiacol-producing bacteria to create a sample, (b) placing the sample in a sterile container, (c) incubating the sample in the sterile container at about 30°C to about 60°C, (d) determining whether the sample has undergone a color change, an odor change, and / or a change in clarity following step (c), and (e) determining whether the liquid contains a guaiacol-producing bacteria based upon the determination in step (d). In embodiments, the liquid that is potentially contaminated with one or more guaiacol-producing bacteria is an acidic fruit juice. In embodiments, the guaiacol-producing bacteria are guaiacol-positive thermophilic acidophilic bacteria.

The present invention relates to a leaching method for leaching mixed powder of electronic waste using Lactobacillus plantarum, and belongs to the field of resource recovery technology. The leaching method described in the present invention comprises the following steps: preparing mixed powder of electronic waste, configuring culture medium, acclimating bacteria, and leaching. The present invention applies Lactobacillus plantarum to the biological leaching of a mixed material of waste lithium iron phosphate batteries and waste printed circuit boards, thereby achieving the extraction of lithium and iron from waste lithium iron phosphate batteries and the extraction of copper from waste printed circuit boards. Compared with traditional biological leaching of single electronic waste, the present method does not use strong acidophilic bacteria, does not produce strongly acidic wastewater, and does not add additional chemical reagents, which greatly reduces the water and soil acidification problem caused by traditional strong acidophilic bacteria, and can achieve efficient biological leaching of a mixed material of waste lithium iron phosphate batteries and waste printed circuit boards without producing a large amount of strong acid that is harmful to the environment.

The present invention discloses C 14 The invention discloses a method for regulating acidophilic bacteria leaching, which belongs to the field of biological leaching technology. The invention finds that different types of AHL signal molecules can have different effects on the growth and leaching of acidophilic bacteria at different concentrations and different addition times. The signal molecule control experiment confirms that C 14 At certain concentrations, the HSL signal molecule can promote and enhance the production of extracellular polysaccharides and fulvic acid by the strain, as well as its ability to adhere to minerals in the later stages of leaching, thereby increasing the strain's growth rate and mineral leaching efficiency. By regulating the concentration of the signal molecule, the rate of acidophilic leaching and its growth can be adjusted. This method is simple to operate, has low production costs, and is environmentally friendly. This method solves the problem of difficult control during mixed acidophilic leaching, promotes the industrial development of biometallurgical technology, and improves leaching production efficiency.

The application discloses a method for leaching waste lithium battery positive pole powder in a neutral environment by using common desulfurization vibrio, and belongs to the field of waste battery metal resource recycling and bio-metallurgical technology. The common desulfurization vibrio obtained through gradient domestication is inoculated into a modified culture medium, and then the bacterial liquid is activated to construct a bioleaching system, so that the valuable metals in waste NCM, NCA, LFP, LCO, LMO and other positive pole materials are bioleached. The common desulfurization vibrio is innovatively used as the leaching strain, and the dependence on acidophilic bacteria in traditional bioleaching is abandoned, so that the efficient dissolution and directional separation of valuable metals in various waste LIBs positive pole materials are realized under mild neutral conditions, and a new microbial technical scheme is provided for waste LIBs positive pole valuable metal recovery.

The application discloses application of a microbial composition in industrial hazardous waste treatment and belongs to the technical field of industrial hazardous waste treatment and comprises the following steps: S1, collecting industrial hazardous waste, obtaining hazardous waste materials after pretreatment of the industrial hazardous waste, and filling the hazardous waste materials in a fermentation tank; S2, adding a growth medium in a bioreactor, connecting domesticated bacterial liquid to the bioreactor for expansion culture, and obtaining fermentation materials of compound acidophilic bacteria; S3, adding a biosurfactant to the fermentation materials of the compound acidophilic bacteria, uniformly stirring, and obtaining cell membrane surface hydrophobic modified compound acidophilic bacteria; and S4, then adding an acidic pH buffer solution to obtain a to-be-treated material. The application can maintain the stability of the pH required by the acidophilic bacteria in the process of treating the industrial hazardous waste and improve the cell membrane surface hydrophobicity of the compound acidophilic bacteria.

The present invention discloses a method for improving the acid tolerance of E. coli by integrating the molecular chaperone CbpA of extreme acidophilic bacteria, belonging to the fields of biochemistry and molecular biology. The present invention provides a molecular chaperone protein CbpA from Acidithiobacillus thermophilum acidophilus and its encoding gene; the molecular chaperone protein CbpA can improve the acid stress resistance of Escherichia coli, and through gene editing technology, the genome of E. coli BL21 is modified to obtain recombinant Escherichia coli. By detecting the growth status and organic acid tolerance of wild-type BL21, defective BL21-ΔcbpA, and recombinant strain BL21-ΔcbpA / AccbpA strains under acidic conditions, its acid tolerance was confirmed. The present invention lays a foundation for later improving the acid tolerance performance of engineering strains through macromolecular repair means such as molecular chaperone proteins, so as to improve the robustness of industrial microorganisms.

The invention belongs to the technical field of biology, and particularly relates to a method for improving acid-eating bacterium tolerance and degrading high-concentration pyridine. According to the specific technical scheme, the method for improving the tolerance and pyridine degradation capacity of the acidovorax sp. Comprises the following steps: extracting a corncob medullary layer leaching solution, and adding the corncob medullary layer leaching solution into a growth environment of the acidovorax sp. According to the invention, it is found for the first time that the corncob medullary layer leachate can effectively improve the tolerance and high-concentration pyridine degradation capability of acidovorax. By adding the corncob medullary layer leachate in the culture environment of the acidovorax bacteria, the acidovorax bacteria can degrade 99% or more of about 2700 mg / L of pyridine after being treated for about 84 hours. The method provided by the invention can effectively improve the high-concentration pyridine degradation effect of the acidovorax LD-B, the method is simple, and the used corncob is an agricultural waste, is wide in source and low in price, has both environmental friendliness and sustainability, greatly reduces the application cost, and has huge popularization value.

This application provides a solid culture medium for the isolation and purification of thermophilic acidophilic bacteria, its preparation method, and its application. The solid culture medium is prepared by mixing a nutrient composition, a coagulant, and deionized water. The coagulant accounts for 9% to 50% of the solid culture medium by mass. The coagulant is a high-acyl gellan gum, containing 3 to 4 acyl groups. The concentration of the high-acyl gellan gum is 5 g / L to 30 g / L. This solid culture medium is made using high-acyl gellan gum. By adjusting the mass percentage of the high-acyl gellan gum, especially by limiting its concentration, the solid culture medium can remain stable for a long time under high temperature and high acid conditions, maintaining good gelation properties. This provides a stable high-temperature and high-acid growth environment for thermophilic acidophilic bacteria, thus facilitating the successful isolation and purification of these bacteria. Furthermore, high-acyl gellan gum is widely available and inexpensive, further reducing culture costs.

The invention belongs to the field of solid waste treatment, and discloses a Fenton iron mud resource utilization method and system. The method comprises the following steps: feeding the Fenton iron sludge into the first bioreactor; under the anaerobic condition, the dissimilatory iron reducing bacteria produce electrons through metabolism of a carbon source, ferric iron is reduced into ferrous iron, meanwhile, the carbon source is subjected to hydrolysis and acidification, small-molecule organic acid, CO2 and H2 are generated, supernate containing ionic state Fe < 2 + > and the small-molecule organic acid is obtained and fed into a second bioreactor, an acid environment and a small-molecule carbon source are provided for iron reducing acidophilic bacteria, and the iron reducing acidophilic bacteria are obtained. Ferric iron is reduced into ferrous iron, and supernate containing ionic state Fe < 2 + > is obtained; and carrying out solid-liquid separation on the supernatant containing the ionic state Fe < 2 + > to obtain an acidic filtrate, and sending the acidic filtrate back to the Fenton reactor as a ferrous iron source. According to the method, excessive COD is not generated and flows back to the Fenton reactor, and Fe < 2 + > ion state recovery is realized without additionally adding acid.

The invention is applicable to the technical field of agricultural disease prevention and control, and provides a method for efficiently and nondestructively treating seeds carrying bacterial fruit blotch pathogenic bacteria and application, the method comprises the following steps: S1, treating the seeds carrying diseases by using low-temperature plasma; s2, performing molecular biological detection on the treated seeds; s3, sowing the treated seeds into a seedling raising tray for seedling raising; a dielectric barrier discharge (DBD) device is adopted in the low-temperature plasma treatment in the step S1. The low-temperature plasma treatment method has an obvious killing effect on watermelon acidophilic bacteria inside and outside the seeds, the seed treatment efficiency is improved, the purpose of preventing and controlling the melon bacterial fruit blotch is achieved, the risk of the diseases is effectively avoided, and huge economic losses caused by the fruit blotch of farmers and enterprises are reduced; the seed vigor can be enhanced, the bud vigor and bud rate of the watermelon seeds can be remarkably improved, the germination and growth period of the seeds can be shortened, the seedling emergence uniformity of the seeds can be improved, and the seedling commodity can be improved.

The present disclosure provides compositions, methods, and kits for detecting guaiacol-producing microorganisms, in particular, Thermophilic Acidophilic Bacteria (TAB) in foodstuff. In one example, a method comprises: (a) contacting a set of oligonucleotides with the sample, wherein the set of oligonucleotides comprising: a first collection of primers targeted for nucleic acids of a guaiacol-producing microorganism; and a second collection of primers targeted for nucleic acids of Alicyclobacillus acidoterrestris; (b) amplifying DNA in the sample with the said set of oligonucleotides under a multiplex polymerase chain reaction (PCR); and (c) determining the presence of PCR products of step (b), wherein the presence of a PCR product in the sample is indicative of contamination of the sample by both guaiacol-producing microorganism and Alicyclobacillus acidoterrestris.