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Microbial insecticide for controlling wireworm and application thereof

A technology of nematicides and microorganisms, which is applied in the directions of nematicides, biocides, and applications, and can solve the problems of large environmental pollution, low output, and limited wide application.

Inactive Publication Date: 2009-10-07
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical synthesis of ALA is complicated, with low output, high cost, and great environmental pollution. It is rarely used in actual production, which also limits its wide application.

Method used

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  • Microbial insecticide for controlling wireworm and application thereof
  • Microbial insecticide for controlling wireworm and application thereof
  • Microbial insecticide for controlling wireworm and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1, the preparation of nematode fermented liquid:

[0034] (1) Strain cultivation: with acidophilic Rhodobacter paella strain PSB-1 ​​(Rhodoblastus acidophilus, its deposit registration number is CCTCC M 206124) as bacterial classification, it is inserted in the transparent glass bottle that contains 100ml liquid medium, Cultivate for 5 days at 30° C. under 2000 LX light, and shake the culture bottle every 8 hours. The liquid medium components are: sodium acetate 1640mg / L, MgSO 4 ·7H 2 O 200mg / L, K 2 HPO 4 900mg / L, KH 2 PO 4 600mg / L, CaCl 2 2H 2 O 75mg / L, EDTA 20mg / L, (NH 4 ) 2 SO 4 1320mg / L, FeSO 4 ·7H 2O 11.8mg / L, trace element solution 1mL / L, pH 6.8 (adjusted with NaOH), trace element solution: H 3 BO 3 2800mg / L, MnSO 4 4H 2 O 2100mg / L, Na 2 MoO 4 2H 2 O750mg / L, ZnSO 4 ·7H 2 O 240mg / L, Cu(NO 3 ) 2 ·3H 2 O 40mg / L.

[0035] (2) Preparation of liquid fermented product: Insert 100 ml of cultured seed microorganisms into a transparent glass bottle ...

Embodiment 2

[0040] 1. The preparation of the nematicidal fermented liquid is the same as in Example 1.

[0041] 2. From July to December 2004, the control test of tomato root-knot nematode was carried out in a greenhouse in a protected area, and the application rate of nematicide fermentation liquid (ALA content was more than 20mg / L) was 90L / hm 2 One week before planting seedlings, dilute 50-200 times with water to carry out soil hole application treatment, the control effect reaches 90%, the tomato yield is increased by more than 80%, and the yield increase rate is higher than that of chemical pesticide methyl bromide (39% yield increase).

[0042] The Escherichia coli DH5α fermentation broth that cannot synthesize ALA was used as a control, and the results showed that it was basically ineffective; while the pure ALA product was used as a positive control, the application concentration was 0.1 g / L-0.5 g / L, and the application rate was 90 L / hm 2 , diluted 50 to 200 times before transplant...

Embodiment 3

[0044] In May 2005, a control experiment of soybean cyst nematode was carried out in a certain greenhouse. The fermented liquid of nematicide was diluted 50 times (ALA content was above 0.5mg / L) to irrigate the treated soil, and the application rate was 90L / hm 2 , irrigate 300-500mL of rhizosphere soil for each plant, and water again after 10-15 days. The test results showed that the reduction rate of cysts was 70.9%, which was higher than that of chemical pesticides applied in the same period.

[0045] The Escherichia coli DH5α fermentation liquid that cannot synthesize ALA was used as a control, and the results showed that it was basically ineffective; the ALA pure product was used as a positive control, the application concentration was 0.1 g / L-0.5 g / L, and the application rate was 90 L / hm 2 , the same treatment of soil root irrigation 2 times, the control effect was 60.4% to 90%.

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Abstract

The invention relates to a microbial nematicide. Microbial nematicides are the fermentation products of the photosynthetic bacterium Rhodoblastus acidophilus CCTCC M 206124, in which 5-aminolevulinic acid (5-Aminolevulinic acid or δ -Aminolevulinic acid, referred to as ALA). The biological nematocide of the present invention is a pure biological preparation, and can be used for biological control of plant parasitic nematodes such as pine wood nematodes, root-knot nematodes, soybean cyst nematodes, and sweet potato stem nematodes. The nematocide of the invention has no adverse effects on the environment and all organisms such as plants, humans and animals, has low production cost, simple production process and does not pollute the environment. Microbial nematicides have no phytotoxicity to tobacco, pepper, cucumber and ramie within the test concentration range, but can promote and stimulate the growth of these crops, with no residue and good safety. The invention has extremely important significance for the biological control of plant nematodes, the development and application of microbial nematicides and the pollution-free production of agricultural products.

Description

technical field [0001] The invention relates to microbial metabolites, in particular to a microbial nematocide. Background technique [0002] Plant parasitic nematodes are a serious plant disease, causing losses of up to 104.4 billion US dollars to crops every year. At present, the common control strategy used in production is chemical control. However, since most chemical nematicides are highly toxic and high-residue pesticides, long-term use will pollute the environment and groundwater sources, and eventually endanger human health through the food chain. Therefore, the research and development of high-efficiency, low-toxicity and biological nematicides has become an important issue in the sustainable development of agriculture. [0003] In many years of nematode biological control research, people have developed direct use of nematode natural enemies (such as fungi, bacteria, viruses, rickettsia, actinomycetes, predatory nematodes, planarians and protozoa) and some toxin-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P5/00
Inventor 成飞雪刘勇张德咏罗源华程菊娥何明远张战泓戴建平朱春晖
Owner HUNAN PLANT PROTECTION INST
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