67 results about "Rickettsia" patented technology

Methods are disclosed for sterilizing tissue to reduce the level of one or more active biological contaminants or pathogens therein, such as viruses, bacteria, (including inter- and intracellular bacteria, such as mycoplasmas, ureaplasmas, nanobacteria, chlamydia, rickettsias), yeasts, molds, fungi, spores, prions or similar agents responsible, alone or in combination, for TSEs and / or single or multicellular parasites. The methods involve sterilizing one or more tissues with irradiation.

A method of stimulating an immune response in a human against malignant cells or an infectious agent comprises the step of administering to the human an immunogenic amount of a primate anti-idiotype antibody or antibody fragment that acts as an immunogenic functional mimic of an antigen produced by or associated with a malignant cell or an infectious agent. Sub-human primate anti-idiotype antisera, especially from baboons, are preferred. Such anti-idiotype antibodies are used to make vaccines for inducing preventive immunity or a therapeutic immune response against tumors, viruses, bacteria, rickettsia, mycoplasma, protozoa, fungi and multicellular parasites.

This invention relates to the original position cross checking method of a Hence Kong waters disease type Pamela Ci's body and its reagent box. This invention includes the following steps: composing the three oligoribonucleotides probes of the High Sim markings according to the special DNA list design of the Pamela Ci's body's 16S rDNA list in the bacteria taxonogy, this unusual probe crosses and combines with the Pamela Ci's body 16s rRNA of the Hence Kong waters slice up, and then enlarges signal through the antigen antibody reaction, and displays colour through enzyme reaction. This checking method can combine the pathogeny checking with its pathology together; it can analyze the infection rate and infection intension on the stylebook slice when effiently and correctly checking the Pamela Ci's body; and the checking result is sensitive and precisious; and the slice after being checked can be preserved perennially.

The invention relates to a method for preparing a veterinary oxytetracycline-artemisinin injection and clinical application of the veterinary oxytetracycline-artemisinin injection. Raw materials of the injection mainly comprise oxytetracycline, artemisinin, trimethoprim, flunixin meglumine, magnesium oxide or magnesium chloride, an antioxidant, a pH regulator, a compound organic solvent and water. As a veterinary compound preparation, the veterinary oxytetracycline-artemisinin injection is mainly used for treating infectious diseases and secondary infection which are caused by sensitive gram positive bacteria and negative bacteria, eperythrozoon, rickettsia and mycoplasma. The method for preparing the veterinary compound oxytetracycline-artemisinin injection is simple, the production process is mild, the chelating temperature is low, the stability is high, and the injection is convenient to use and is suitable for industrialized production.

The invention discloses a real-time fluorescence quantitative polymerase chain reaction (PCR) kit for five kinds of pathogens such as incorporeity, rickettsia and the like and a method thereof. Specific oligonucleotide primers and probes are designed according to the specific gene nucleotide sequences of the five kinds of pathogens such as the incorporeity, the rickettsia and the like. The invention provides a method for detecting the incorporeity and the rickettsia and a high-sensitivity detection kit consisting of the specific primers. The kit is easy, convenient and quick in operation, andhas high sensitivity and specificity. The kit can perform rapid specific detection on 5 kinds of related rickettsia and is applicable to clinical laboratories and disease monitoring tissue laboratories. The invention also discloses a method for detecting the incorporeity and the rickettsia by using the kit.

The invention relates to a gene chip for detecting various prawn pathogenes and a detection method thereof. The gene chip is prepared by arranging the probes of a quality control system and the probe points of 18 types of prawn pathogenes on the same solid-phase carrier nylon membrane. The probes of 18 types of the prawn pathogenes comprise probes for detecting 15 types of viruses, probes for detecting fusarium, fish-killing aphanomyces and deformation algal filament cristatum, and probes for detecting Paranophrys and prawn rickettsia. When the gene chip is used for detection, a signal is judged according to the condition whether blue or royal purple appears at a hybridization point by using a digoxin hybridization chromogenic method. The construction of the quality control system on the gene chip ensures the detection specificity and sensitivity, thus, the gene chip can be used for simultaneously detecting various prawn pathogenic microorganisms on the same nylon membrane, has the characteristic of high flux, can replace the prior related detection method for the prawn pathogenic microorganisms, and is a breakthrough of the prior prawn pathogenic microorganism judging technique.

A method of stimulating an immune response in a human against malignant cells or an infectious agent comprises the step of administering to the human an immunogenic amount of a primate anti-idiotype antibody or antibody fragment that acts as an immunogenic functional mimic of an antigen produced by or associated with a malignant cell or an infectious agent. Sub-human primate anti-idiotype antisera, especially from baboons, are preferred. Such anti-idiotype antibodies are used to make vaccines for inducing preventive immunity or a therapeutic immune response against tumors, viruses, bacteria, rickettsia, mycoplasma, protozoa, fungi and multicellular parasites.

The disclosure relates to outer membrane vesicles from Francisella and Piscirickettsia, and their use in vaccine compositions. In particular, the present disclosure relates to compositions and methods useful in inducing protective immunity against francisellosis or salmon rickettsial septicaemia (SRS) in fish.

The invention discloses a nucleic acid extraction pretreatment amplification system. The system comprises a filtering device, a DNA extraction device, a uniform mixing device, a PCR amplification device and a driving device for driving a liquid to flow. The labor time is saved and the working efficiency of people is improved through a method of sequentially treating samples by different reagents.Through ingenious pipeline design, functional units such as the filtering device, the DNA extraction device for nucleic acid extraction, the uniform mixing device and the PCR amplification device areintegrated in an embedded manner, so that the overall size is remarkably reduced. The system has the advantages of being simple in structure, easy to achieve and operate, low in cost and capable of being widely used and popularized. Besides, the PCR amplification device is based on temperature circulation, so that rapid amplification and signal amplification of polymerase on nucleic acid moleculesare realized, and the detection time is greatly shortened. In addition, measurable reagents are wide in variety, for example, bacteria, viruses, fungi, chlamydia, rickettsia and the like can be rapidly detected.

A method of treating plant and animal systems and inanimate surfaces for the purposes of controlling plant pests, introducing pesticides and nutrients into plants, mitigating frost damage to plants, increasing crop yields, controlling certain plant diseases, controlling arthropod, bacterial, fungal, mycoplasma, rickettsia, and viral pests of animals and humans, and disinfecting inanimate surfaces. The method utilizes the unique multi-directional dispersion property of the tannate complex of picro ammonium formate and the tannate complex of picro cupric ammonium formate, in aqueous solution, combined with a minor amount of a surfactant sufficient to prevent formation of ammonium picrate, to penetrate plant and animal systems and inanimate surfaces and travelling multidirectionally therein. The method is carried out by introducing a small but effective amount of the tannate complex to the plant or animal system or inanimate surface.

The invention relates to a primer, a probe and a kit for detecting and typing a rickettsia by virtue of real-time FRET-PCR (Fluorescent Resonance Energy Transfer-Polymerase Chain Reaction) and nested PCR. The primer and the probe are shown as sequences 1-8. By virtue of designing the primer and the probe of the FRET-PCR, nucleinic acids of all Rickettsiaes can be specifically amplified, thus fast judging positive samples; then two pairs of primers of the nested PCR can be designed by selecting other conservative intervals of the same gene, and the Rickettsiae of the corresponding positive samples can be determined by virtue of nested PCR, agarose gel electrophoresis and sequencing. Therefore, the system not only can fast and specifically detect the rickettsia organisms of all the Rickettsiaes, but also can confirm the Rickettsiae.

A degerming dustproof a multifunctional electronic mask is characterized in that an outer layer body 1, an inner layer body 2, an isolation layer 3, an air hole 4, an air hole 5, an air hole 6, a negative electrode 7, an adsorption body 8, a positive electrode 9, an adsorption body 10, a supporting body 11, ear bands 12, a power supply 13 and a zipper 14 are connected into a whole to form the degerming and dustproof multifunctional electronic mask. The degerming dustproof multifunctional electronic mask which is wide in degerming range and various in dustproof variety. Degerming comprises non-cell microorganisms (various viruses); prokaryotic cell type microorganisms (various bacteria, mycoplasma, chlamydia, rickettsia, spirochete and actinomycetes); eukaryotic cell type microorganisms (fungi) and various bacterial spores. Dust prevention includes a variety of oily, non-oily contaminants in air from particles greater than 5 microns to particles less than 1 micron. The degerming dustproof multifunctional electronic mask is suitable for medical treatment and public health, production labor, household protection, fitness and physical exercise. The degerming dustproof multifunctional electronic mask is wide in manufacturing material and low in production cost, the mask body is made of elastic non-breathable ultrathin insulating material fibers, fabrics and films, the electrodes aremade of metal sheets, metal coating films or conductive carbon fibers, and the adsorption body is made of paper fibers (used in dry seasons in winter) or chemical fibers (used in rainy seasons in summer). The power supply is a general mobile phone lithium battery and a high-voltage electron generator.

Methods are provided for treating or preventing infections by obligate intracellular prokaryotes, including mycoplasma, rickettsia and chlamydia, and DNA viruses, including herpes viruses, papillomaviruses, adenoviruses and hepatitis B virus. The methods involve the administration of 3:1 complexes of 3-hydroxy-4-pyrones with gallium, e.g., gallium maltolate. Therapies incorporating gallium maltolate in combination with agents used against obligate intracellular prokaryote and DNA virus pathogens are also provided, as are multi-combination therapies designed to treat co-infection by an obligate intracellular prokaryote or DNA virus in an immunocompromised individual. These multi-combination therapies rely on the ability of gallium maltolate to complement antiviral medication regimes against both HIV and other pathogens such as herpesvirus infections, including Kaposi sarcoma, CMV retinitis and blindness, and lymphomas, in patients immunocompromised by HIV infection.

The invention discloses a dual real-time fluorescence quantitative PCR (polymerase chain reaction) detection system and method for Rickettsia mooseri and Orientia tsutsugamushi. The detection system is composed of primers, probes, a TaqMan mix buffer, ddH2O, pMD18-T-Rm and pMD18-T-Ot, the primers include Pr47F, Pr47R, Ot-F and Ot-R, and the probes include a Probe-P and a Probe-O. The primers and the probes have the advantages of high specificity and high sensitivity. The detection method does not have cross reaction with Rickettsia prowazeki, Rickettsia rickettsii and Q fever rickettsia, and 8.24 copied Rickettsia mooseri standards and 43.6 copied Orientia tsutsugamushi standards can be detected at the same time. The dual real-time fluorescence quantitative PCR detection system and method can be used for detecting Rickettsia mooseri and Orientia tsutsugamushi at the same time and is suitable for frontier port health quarantine departments to conduct monitoring and detecting work of the above pathogens.

Targeted disruption of a specific gene and its subsequent restoration in obligate intracellular bacteria remains extremely challenging due to their absolute requirement for residence inside a host cell to replicate. Here, targeted allelic exchange mutations were created to inactivate two genes and then to restore one of the two genes of a rickettsial pathogen, Ehrlichia chaffeensis. These methodswere then also successfully utilized in Ehrlichia canis and Anaplasma phagocyophilum. The resultant mutated pathogens are useful in immunogenic compositions for reducing the incidence of or severity of infection with ricksettsial pathogens.

The invention discloses an LAMP primer group, kit and method for rapid identification of tsutsugamushi disease rickettsia. The detection primer group comprises a pair of outer primers, a pair of inner primers and a pair of loop primers. The detection kit comprises primer liquid, reaction liquid, DNA polymerase, reverse transcriptase and a contrasting and color developing agent. The detection method comprises the steps that by extracting the pathogene RNA to be detected, amplification is conducted on a sample RNA template at the temperature of 60-65 DEG C by means of the reverse transcription activity of the reverse transcriptase through six specific primers and a DNA polymerase having the strand displacement activity, and whether amplification is achieved or not is judged by adding the color developing agent and observing the change of color in a reaction tube. The LAMP primer group, kit and method for rapid identification of the tsutsugamushi disease rickettsia have the advantages of being rapid, efficient, easy and convenient to operate, high in specificity, high in sensitivity, capable of achieving identification easily and conveniently, suitable for field detection and the like, and the LAMP primer group, kit and method for rapid identification of the tsutsugamushi disease rickettsia are suitable for application and popularization.

The invention relates to a long-acting terramycin injection and a preparation method thereof. The injection comprises, by weight, 10-40 parts of terramycin, 10-40 parts of dimethyl formamide, 5-10 parts of ethanolamine, 10-30 parts of propylene glycol, 1-2 parts of sodium formaldehyde thiosulfate, 20-30 parts of anhydrous ethanol, 10-20 parts of tween-80, 1-8 parts of a sustained release agent and 1-5 parts of water for injection. The long-acting terramycin injection has the advantages of scientific compatibility, advanced technology, lasting drug effect, reasonable composition, wide effect and use safety, is a long-acting preparation with small irritation, and is mainly used for preventing and treating infections induced by Gram-negative bacteria, Gram-positive bacteria, spirochetes, actinomyces, mycoplasmas, rickettsiae and other protozoan.

The present invention relates to a traditional Chinese medicine disinfection wet tissue, wherein the traditional Chinese medicine component raw materials comprise, by weight, 20-30 parts of licorice root, 10-20 parts of obtuseleaf senna seed, 2-6 parts of dichondra repens forst, 2-6 parts of Qihua, 5-10 parts of andrographis paniculata, 10-25 parts of honeysuckle, 2-10 parts of peppermint, 5-15 parts of rhizoma imperatae, and 2-8 parts of cornus officinalis. According to the present invention, the traditional Chinese medicines of the traditional Chinese medicine disinfection wet tissue comprise the licorice root, the dichondra repens forst and the andrographis paniculata, and the wet tissue prepared by extracting the traditional Chinese medicines has the good antibacterial effect, provides good inhibition effects on escherichia coli, bacteria, viruses, spirochaeta, rickettsia, chlamydia, mycoplasma and the like, further has good odor and good color, and is suitable for people with different ages.

The invention relates to application of aerosol allicin to prevention and treatment of new coronavirus infection and other respiratory tract infectious diseases. The research finds that the new coronavirus only invades and infects respiratory tract epithelial cells expressing ACE2 and TMPRSS2; aerosol allicin can effectively inhibit TMPRSS2, so that new coronavirus is prevented from entering and infecting cells, infection factors in alveolar air can be eliminated, and new coronavirus infection can be effectively prevented and treated. In addition, the invention also finds that the aerosol allicin can effectively prevent and treat respiratory system diseases caused by infection factors of bacteria (such as mycobacterium tuberculosis, and Yersinia pestis), fungi (such as candida albicans andaspergillus niger), parasites (such as amoeba, giardia and leishmania), rickettsia and mycoplasma, and other viruses (such as influenza A, influenza B, encephalitis B, SARS, MERS, avian influenza and African swine fever) of non-SARSARS-CoV-2. Clinical application prospect is wide.

The invention discloses a new disinfection material for a wet wipe and a preparation method of the new disinfection material. The wet disinfection wipe is made of a fiber tower by adsorbing the disinfection material. The disinfection material is prepared from the raw materials in parts by weight as follows: 3-8 parts of tea polyphenol, 20-35 parts of herba lobeliae chinensis extracts, 10-20 parts of inula japonica extracts, 10-20 parts of incense tube herb extracts, 2-6 parts of turpentine, 3-5 parts of limonene, 10-20 parts of pseudechinolaena polystachya and 60-100 parts of a 70% ethanol aqueous solution. The new disinfection material for the wet wipe adopts a simple preparation method, is convenient to use, can kill a variety of pathogenic bacteria such as staphylococcus aureus, viruses, spirochete, rickettsia, chlamydiae, mycoplasmas, fungi, actinomycetes and the like and has high killing efficiency.

A composition comprises bacteriophage covalently attached to an edible particle and is for use in treating bacterial infection in fish or crustaceans. Infections in fish or crustaceans caused by Vibrio, Aeromonas, Yersinia, Moritella, Rickettsia, Piscirickettsia, Lactococcus, Pseudomonas, Flavobacterium or Photobacterium bacteria species can be treated. Bacteria infected with a lysogenic bacteriophage are used for treating disease of fish or crustaceans caused by similar infections by bacteria carrying lysogenic bacteriophage that express a toxin gene.

The invention relates to the field of biology, in particular relates to a detection primer set of bainite rickettsia and a detection reagent of the detection primer set and a real-time fluorescent quantitative PCR method. The primer set comprises an upstream primer as shown in SEQ ID NO:1 and a downstream primer as shown in SEQ ID NO:2. The method for carrying out real-time fluorescent quantitative PCR by the primer set provided by the invention is high in amplification efficiency, small in error, and objective and accurate in result, and has good specificity, repeatability and sensitivity.

The invention provides a composite aureomycin and chloramphenicol nano emulsion as well as a preparation method and application thereof. The nano emulsion comprises the following raw materials: a surfactant, oil, aureomycin, chloramphenicol and water. The nano emulsion is simple in preparation process, free of phase separation or precipitate, and high in storage stability. The composite aureomycinand chloramphenicol nano emulsion provided by the invention is high in antibacterial activity, relatively good in aging stability, thermal storage stability, anti-freezing stability and accelerationstability, is free of conspicuous stimulus response phenomenon, is a wide-spectrum antibiotic, and can be applied to treatment on infections caused by gram-positive bacteria, gram negative bacteria, rickettsia, Chlamydia, mycoplasma, spirochete, actinomycetes and protozoons. The dosage of the medicine can be reduced, drug resistance of bacteria can be reduced or avoided, a slow release property isachieved, the medicine action time is prolonged, the toxic and side effects of the medicine are reduced, the bioavailability and the stability of the medicine are improved, and medicinal effects canbe brought into play sufficiently.

A composition comprises bacteriophage covalently attached to an edible particle and is for use in treating bacterial infection in fish or crustaceans. Infections in fish or crustaceans caused by Vibrio, Aeromonas, Yersinia, Moritella, Rickettsia, Piscirickettsia, Lactococcus, Pseudomonas, Flavobacterium or Photobacterium bacteria species can be treated. Bacteria infected with a lysogenic bacteriophage are used for treating disease of fish or crustaceans caused by similar infections by bacteria carrying lysogenic bacteriophage that express a toxin gene.

The invention provides a method for producing a dustless aseptic environment-friendly gertrude, which comprises the steps of preparing finished underclothes from cotton cloth, and is characterized by also comprising three steps of washing the garment, performing ultraviolet sterilization and packaging under vacuum. In the method, ozone liquid for sterilizing the dustless aseptic underclothes has the high oxidation capacity, and oxygen atoms can oxidize cell walls of bacteria and even penetrate through the cell walls to perform chemical combination with unsaturated bonds in the cell walls to kill the bacteria, so the bacteria are killed by the high reduction potential of the ozone liquid. Therefore, the method has the advantages of removing and inhibiting rickettsia bacteria, chlamydia pathogenic bacteria, mycoplasma pathogenic bacteria, spirochete pathogenic bacteria and the like effectively, killing various pathogenic virus such as influenza virus, hepatitis C virus, human immunodeficiency virus (HIV), human rotavirus (HRV) and the like and reducing pathophoresis effectively.

The invention relates to the technical field of detection, in particular to primers and a digital PCR kit for detecting infectious endocarditis pathogens. The invention provides a primer probe combination. The primer probe combination comprises any nucleotide sequence as shown in SEQ ID No. 1 to SEQ ID No. 39. The kit is mainly used for detecting 12 pathogens capable of causing infectious endocarditis, namely streptococcus, staphylococcus aureus, coagulase negative staphylococcus, enterococcus, candida albicans, pseudomonas aeruginosa, pseudomonas maltophilia, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, rickettsia and bartonella. Four detection channels and one internal control channel can be detected at a time, 12 irrelevant human common pathogens are not subjected to non-specific amplification, and absolute quantification can be carried out.

The invention discloses a fruit and vegetable cleaning machine capable of air sterilization. The fruit and vegetable cleaning machine capable of air sterilization comprises an electronic sterilizationfilter 1, an ultrasonic generator 2, an ultrasonic waveguide 3, a cleaning cylinder 4, a blow-down valve 5, an exhaust hole 6, a water inlet pipe 7, an upper cover 8 and a machine shell 9 through mechanical connection, circuit connection and pipeline connection. The physical factors produced by a physical method are as follows: various contaminating substances are eliminated, and microorganisms,including pathogenic microorganisms, on the surfaces of foods and in the deep of the foods are inactivated. A sterilization range is wide, and sterilization types are more, including non-cellular microorganisms (various viruses), prokaryotic cell microorganisms (various bacteria, mycoplasma, chlamydia, rickettsia, spirochete, and actinomycetes) and eukaryotic cell microorganisms (fungi), and bacterial spore can be inactivated under the ambient condition or by matching with an appropriate chemical agent for lysis. The fruit and vegetable cleaning machine capable of air sterilization is simple in structure, convenient to use and low in cost, is relatively economical and can realize a large number of production and manufacturing and family application by having a brushless direct-current motor (BLDCM) manufacturing assembly line.

The invention discloses an intelligent disinfection box, and belongs to the field of disinfection and sterilization equipment. The intelligent disinfection box comprises a cover body and a box body, wherein the cover body is covered on the box body; an ultraviolet ray disinfection device is arranged in the cover body, and is used for performing sterilization and disinfection treatment on articlesin the box body; and a wireless charging device is arranged in the box body, and is used for performing wireless charging on electronic equipment. According to the intelligent disinfection box disclosed by the invention, when the intelligent disinfection box is uncovered, sterilization and disinfection treatment can be automatically disconnected; when the intelligent disinfection box is not uncovered, sterilization is automatically connected, and intelligence is realized; and besides, an UVC-LED deep ultraviolet ray illuminant is arranged, and inherited substances of DNA and the like are destroyed, so that bacterial reproduction bodies, spores, mycobacterium, viruses, fungi, rickettsia, mycoplasma and the like can be efficiently destroyed, and the intelligent disinfection box has broad spectrum properties.