67 results about "Rickettsia" patented technology

A method of stimulating an immune response in a human against malignant cells or an infectious agent comprises the step of administering to the human an immunogenic amount of a primate anti-idiotype antibody or antibody fragment that acts as an immunogenic functional mimic of an antigen produced by or associated with a malignant cell or an infectious agent. Sub-human primate anti-idiotype antisera, especially from baboons, are preferred. Such anti-idiotype antibodies are used to make vaccines for inducing preventive immunity or a therapeutic immune response against tumors, viruses, bacteria, rickettsia, mycoplasma, protozoa, fungi and multicellular parasites.

The invention discloses a nucleic acid extraction pretreatment amplification system. The system comprises a filtering device, a DNA extraction device, a uniform mixing device, a PCR amplification device and a driving device for driving a liquid to flow. The labor time is saved and the working efficiency of people is improved through a method of sequentially treating samples by different reagents.Through ingenious pipeline design, functional units such as the filtering device, the DNA extraction device for nucleic acid extraction, the uniform mixing device and the PCR amplification device areintegrated in an embedded manner, so that the overall size is remarkably reduced. The system has the advantages of being simple in structure, easy to achieve and operate, low in cost and capable of being widely used and popularized. Besides, the PCR amplification device is based on temperature circulation, so that rapid amplification and signal amplification of polymerase on nucleic acid moleculesare realized, and the detection time is greatly shortened. In addition, measurable reagents are wide in variety, for example, bacteria, viruses, fungi, chlamydia, rickettsia and the like can be rapidly detected.

A method of treating plant and animal systems and inanimate surfaces for the purposes of controlling plant pests, introducing pesticides and nutrients into plants, mitigating frost damage to plants, increasing crop yields, controlling certain plant diseases, controlling arthropod, bacterial, fungal, mycoplasma, rickettsia, and viral pests of animals and humans, and disinfecting inanimate surfaces. The method utilizes the unique multi-directional dispersion property of the tannate complex of picro ammonium formate and the tannate complex of picro cupric ammonium formate, in aqueous solution, combined with a minor amount of a surfactant sufficient to prevent formation of ammonium picrate, to penetrate plant and animal systems and inanimate surfaces and travelling multidirectionally therein. The method is carried out by introducing a small but effective amount of the tannate complex to the plant or animal system or inanimate surface.

Methods are provided for treating or preventing infections by obligate intracellular prokaryotes, including mycoplasma, rickettsia and chlamydia, and DNA viruses, including herpes viruses, papillomaviruses, adenoviruses and hepatitis B virus. The methods involve the administration of 3:1 complexes of 3-hydroxy-4-pyrones with gallium, e.g., gallium maltolate. Therapies incorporating gallium maltolate in combination with agents used against obligate intracellular prokaryote and DNA virus pathogens are also provided, as are multi-combination therapies designed to treat co-infection by an obligate intracellular prokaryote or DNA virus in an immunocompromised individual. These multi-combination therapies rely on the ability of gallium maltolate to complement antiviral medication regimes against both HIV and other pathogens such as herpesvirus infections, including Kaposi sarcoma, CMV retinitis and blindness, and lymphomas, in patients immunocompromised by HIV infection.

The invention discloses a dual real-time fluorescence quantitative PCR (polymerase chain reaction) detection system and method for Rickettsia mooseri and Orientia tsutsugamushi. The detection system is composed of primers, probes, a TaqMan mix buffer, ddH2O, pMD18-T-Rm and pMD18-T-Ot, the primers include Pr47F, Pr47R, Ot-F and Ot-R, and the probes include a Probe-P and a Probe-O. The primers and the probes have the advantages of high specificity and high sensitivity. The detection method does not have cross reaction with Rickettsia prowazeki, Rickettsia rickettsii and Q fever rickettsia, and 8.24 copied Rickettsia mooseri standards and 43.6 copied Orientia tsutsugamushi standards can be detected at the same time. The dual real-time fluorescence quantitative PCR detection system and method can be used for detecting Rickettsia mooseri and Orientia tsutsugamushi at the same time and is suitable for frontier port health quarantine departments to conduct monitoring and detecting work of the above pathogens.

Targeted disruption of a specific gene and its subsequent restoration in obligate intracellular bacteria remains extremely challenging due to their absolute requirement for residence inside a host cell to replicate. Here, targeted allelic exchange mutations were created to inactivate two genes and then to restore one of the two genes of a rickettsial pathogen, Ehrlichia chaffeensis. These methodswere then also successfully utilized in Ehrlichia canis and Anaplasma phagocyophilum. The resultant mutated pathogens are useful in immunogenic compositions for reducing the incidence of or severity of infection with ricksettsial pathogens.

The invention relates to application of aerosol allicin to prevention and treatment of new coronavirus infection and other respiratory tract infectious diseases. The research finds that the new coronavirus only invades and infects respiratory tract epithelial cells expressing ACE2 and TMPRSS2; aerosol allicin can effectively inhibit TMPRSS2, so that new coronavirus is prevented from entering and infecting cells, infection factors in alveolar air can be eliminated, and new coronavirus infection can be effectively prevented and treated. In addition, the invention also finds that the aerosol allicin can effectively prevent and treat respiratory system diseases caused by infection factors of bacteria (such as mycobacterium tuberculosis, and Yersinia pestis), fungi (such as candida albicans andaspergillus niger), parasites (such as amoeba, giardia and leishmania), rickettsia and mycoplasma, and other viruses (such as influenza A, influenza B, encephalitis B, SARS, MERS, avian influenza and African swine fever) of non-SARSARS-CoV-2. Clinical application prospect is wide.

The invention discloses a new disinfection material for a wet wipe and a preparation method of the new disinfection material. The wet disinfection wipe is made of a fiber tower by adsorbing the disinfection material. The disinfection material is prepared from the raw materials in parts by weight as follows: 3-8 parts of tea polyphenol, 20-35 parts of herba lobeliae chinensis extracts, 10-20 parts of inula japonica extracts, 10-20 parts of incense tube herb extracts, 2-6 parts of turpentine, 3-5 parts of limonene, 10-20 parts of pseudechinolaena polystachya and 60-100 parts of a 70% ethanol aqueous solution. The new disinfection material for the wet wipe adopts a simple preparation method, is convenient to use, can kill a variety of pathogenic bacteria such as staphylococcus aureus, viruses, spirochete, rickettsia, chlamydiae, mycoplasmas, fungi, actinomycetes and the like and has high killing efficiency.

The invention relates to the field of biology, in particular relates to a detection primer set of bainite rickettsia and a detection reagent of the detection primer set and a real-time fluorescent quantitative PCR method. The primer set comprises an upstream primer as shown in SEQ ID NO:1 and a downstream primer as shown in SEQ ID NO:2. The method for carrying out real-time fluorescent quantitative PCR by the primer set provided by the invention is high in amplification efficiency, small in error, and objective and accurate in result, and has good specificity, repeatability and sensitivity.

The invention provides a composite aureomycin and chloramphenicol nano emulsion as well as a preparation method and application thereof. The nano emulsion comprises the following raw materials: a surfactant, oil, aureomycin, chloramphenicol and water. The nano emulsion is simple in preparation process, free of phase separation or precipitate, and high in storage stability. The composite aureomycinand chloramphenicol nano emulsion provided by the invention is high in antibacterial activity, relatively good in aging stability, thermal storage stability, anti-freezing stability and accelerationstability, is free of conspicuous stimulus response phenomenon, is a wide-spectrum antibiotic, and can be applied to treatment on infections caused by gram-positive bacteria, gram negative bacteria, rickettsia, Chlamydia, mycoplasma, spirochete, actinomycetes and protozoons. The dosage of the medicine can be reduced, drug resistance of bacteria can be reduced or avoided, a slow release property isachieved, the medicine action time is prolonged, the toxic and side effects of the medicine are reduced, the bioavailability and the stability of the medicine are improved, and medicinal effects canbe brought into play sufficiently.

The invention discloses a fruit and vegetable cleaning machine capable of air sterilization. The fruit and vegetable cleaning machine capable of air sterilization comprises an electronic sterilizationfilter 1, an ultrasonic generator 2, an ultrasonic waveguide 3, a cleaning cylinder 4, a blow-down valve 5, an exhaust hole 6, a water inlet pipe 7, an upper cover 8 and a machine shell 9 through mechanical connection, circuit connection and pipeline connection. The physical factors produced by a physical method are as follows: various contaminating substances are eliminated, and microorganisms,including pathogenic microorganisms, on the surfaces of foods and in the deep of the foods are inactivated. A sterilization range is wide, and sterilization types are more, including non-cellular microorganisms (various viruses), prokaryotic cell microorganisms (various bacteria, mycoplasma, chlamydia, rickettsia, spirochete, and actinomycetes) and eukaryotic cell microorganisms (fungi), and bacterial spore can be inactivated under the ambient condition or by matching with an appropriate chemical agent for lysis. The fruit and vegetable cleaning machine capable of air sterilization is simple in structure, convenient to use and low in cost, is relatively economical and can realize a large number of production and manufacturing and family application by having a brushless direct-current motor (BLDCM) manufacturing assembly line.

The invention discloses an intelligent disinfection box, and belongs to the field of disinfection and sterilization equipment. The intelligent disinfection box comprises a cover body and a box body, wherein the cover body is covered on the box body; an ultraviolet ray disinfection device is arranged in the cover body, and is used for performing sterilization and disinfection treatment on articlesin the box body; and a wireless charging device is arranged in the box body, and is used for performing wireless charging on electronic equipment. According to the intelligent disinfection box disclosed by the invention, when the intelligent disinfection box is uncovered, sterilization and disinfection treatment can be automatically disconnected; when the intelligent disinfection box is not uncovered, sterilization is automatically connected, and intelligence is realized; and besides, an UVC-LED deep ultraviolet ray illuminant is arranged, and inherited substances of DNA and the like are destroyed, so that bacterial reproduction bodies, spores, mycobacterium, viruses, fungi, rickettsia, mycoplasma and the like can be efficiently destroyed, and the intelligent disinfection box has broad spectrum properties.