194 results about "Actinomyces" patented technology

The invention relates to the technical field of soil function improvement, in particular to a rice field soil recarburization and capacity expansion method based on a charcoal-based organic fertilizer. The method comprises the following steps: crushing a biomass raw material, pyrolyzing and cooling to obtain biochar; immersing the biochar in a functional bacteria solution containing actinomycetes and bacillus for activating treatment to obtain modified biochar; mixing the modified biochar and decomposed straw, composting and fermenting to obtain organic fertilizers with different particle sizes; the large-particle-size organic fertilizer is applied to deep soil, the soil is covered with soil after straw is laid on the soil, and the small-particle-size organic fertilizer is applied to surface soil. Through layered application, the large-particle-size biochar repairs the deep soil structure and expands the carbon storage capacity, and meanwhile, the carbon stability is improved through carbon-nitrogen synergistic metabolism driven by functional bacteria, so that the carbon immobilization efficiency is improved, meanwhile, soil recarburization and expansion are achieved, the contradiction between carbon sequestration and structure degradation and nutrient imbalance in a traditional recarburization and expansion technology is solved, and the soil recarburization and expansion efficiency is improved. Synergic improvement of a carbon sink function and agricultural productivity is promoted.

The invention discloses a high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation. According to the high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation, yield increase and quality improvement are achieved through fungus material composite strengthening treatment and a composite strain synergistic inoculation technology. The method comprises the following steps: (1) fermenting a tilia brachypoda substrate by lactobacillus plantarum and soaking in a nano-composite nutrient solution to form a high-activity bacterial material; (2) nitrogen-fixing bacteria, disease-resistant actinomycetes and armillaria mellea are mixed and inoculated according to the proportion of 3: 1: 1, nitrogen supply is improved, and the occurrence rate of black rot is reduced; 3) activating a GASA stress-resistant gene of the seed hemp through a pulsed electric field (5 kV / cm, 10 microseconds), and enabling the germination rate to reach 88.5% in combination with a calcium signal channel strengthening technology; according to the method, the yield per mu of gastrodia elata is increased by 30%-40%, the gastrodin content is increased by 30% or above, the chemical pesticide dosage is reduced by 55%, the strain passage purity is stable, the method is suitable for large-scale planting in multiple climate areas, and an innovative solution is provided for green production of traditional Chinese medicinal materials.

The invention discloses a bifidobacterium animalis subsp. Lactis BA-C53 and an application of the bifidobacterium animalis subsp. Lactis BA-C53 in products for improving bone health. The bifidobacterium animalis subsp. Lactis BA-C53 is classified and named as bifidobacterium animalis subsp. Lactis Bifidobacterium animalis subsp. Lactis BA-C53, the preservation number is CCTCC NO: M 2024460, the preservation unit is China Center for Type Culture Collection, and the preservation time is March 11, 2024. The bifidobacterium animalis subsp. Lactis BA-C53 can effectively improve the steady state of intestinal flora of a mouse with osteoporosis induced by estrogen deficiency, so that the relative abundance of phylum firmicalis and phylum actinomycetes is increased, the relative abundance of phylum bacteroides is reduced, and then short-chain fatty acid is generated to activate intestinal immunity and maintain the integrity of an intestinal barrier, so that the osteoporosis caused by estrogen deficiency is inhibited. Meanwhile, osteoclast differentiation can be inhibited by regulating NFATC1, RANKL and OPG pathways, then osteoporosis is relieved, and the compound can be widely applied to preparation of food and drugs.

The invention discloses a preparation method of an actinomycetes and waste mushroom matrix composite, belongs to the technical field of environmental microorganisms, solves the problems of low resource utilization rate, serious environmental pollution and the like in the existing waste mushroom matrix treatment, and overcomes the defects of loose soil structure and poor erosion resistance caused by direct returning of the waste mushroom matrix to the field. The method comprises the following steps: separating actinomycetes strains from soil, purifying and culturing the actinomycetes strains into a bacterial solution, crushing a waste mushroom substrate, adjusting the water content and the initial pH value, inoculating the bacterial solution into a pretreated substrate, and culturing in a dark place to form a composite material with biological activity and structural stability. Lignocellulose in a matrix is efficiently degraded by utilizing cellulase secreted by actinomycetes and lignin peroxidase, and a three-dimensional cross-linked structure is formed through a hypha network and humic acid-minerals, so that the mineralization efficiency of organic matters and soil agglomeration are improved. The composite material can be applied to the field of water and soil loss prevention and control, and efficient conversion of waste resources and collaborative improvement of soil ecological functions are achieved.

The invention relates to a formulation of microbial consortia selected from (a) Bacilli group, (b) Pseudomonas group and (c) Actinomycete group having nitrogen fixing capacity, when the bacteria comes in contact with soil or any aqueous media it gets active, which further helps for plant growth and makes the soil environment more suitable for plant.

The present invention discloses an organic fertilizer for promoting plant growth and soil improvement and a preparation method thereof, and belongs to the technical field of organic fertilizers. The organic fertilizer comprises the following raw materials: decomposed chicken manure, soybean meal, straw powder, sweet potato vine, humic acid, composite microbial agent, rosmarinic acid, and a stimulant. The composite microbial agent is composed of Actinomyces millefolius, Pseudomonas stutzeri, and Bacillus velezensis, and the stimulant is a glucosamine sulfate solution. Rosmarinic acid, glucosamine sulfate solution, and composite microbial agent are added to the organic fertilizer of the present invention. These three components are compounded with straw, chicken manure, etc. to form an organic fertilizer. The synergistic effect of multiple substances promotes the growth of cabbage and increases yield on the one hand; on the other hand, it significantly improves the physical and chemical properties of the soil.

The invention discloses a microbial agent for increasing manure compost temperature and application thereof in high-temperature compost, and belongs to the field of agricultural solid waste treatment. The microbial agent for increasing the manure composting temperature comprises phanerochaete chrysosporium, bacillus stearothermophilus, penicillium oxalicum, streptomyces, common thermoactinomycetes and bacillus subtilis. The livestock padding manure high-temperature clean composting technology is established through adjustment and optimization, the purposes that the temperature of the padding manure is increased fast, the high-temperature period is long, the composting period is shortened, base materials are fully degraded are achieved, reference can be provided for resource utilization of livestock and poultry breeding solid waste, and important application value is achieved.

The invention discloses a polysaccharide polymer, application of the polysaccharide polymer and a preparation method of the polysaccharide polymer. The polysaccharide polymer QPS capable of remarkably selectively inhibiting harmful bacteria in aquaculture is separated and purified from the water mold antagonistic actinomycete strain QHV2 for the first time, can effectively inhibit mycelial growth and spore germination of water mold / branchiomycosis and destroy mature biological membranes, is stable in activity, free of toxic effects on fry and good in biological safety, and can be applied to the field of aquaculture. The material can be used as a candidate material for research and development of a fish pathogenic fungus biological prevention and control agent. Meanwhile, the polysaccharide polymer QPS avoids pollution of harmful substances to a water body when a QHV2 fermentation product is directly applied, can selectively inhibit staphylococcus aureus, saprolegnia, branchiomycosis and other harmful bacteria in aquaculture, and has no inhibition or weak inhibition on pseudomonas pseudoalcaligenes, bacillus and other beneficial bacteria in aquaculture. And the application effect is better.

The invention belongs to the field of microbial agents, and particularly relates to a compost heat production method of a ganoderma lucidum basswood planting waste substrate, which comprises the following steps: mixing the ganoderma lucidum basswood planting waste substrate with chicken manure to obtain a fermentation raw material; paving the fermentation raw materials in a fermentation container layer by layer; a complex microbial inoculant is uniformly sprayed on the surface of each layer of fermentation raw material; the complex microbial inoculant is prepared from the following strains in percentage by weight: 10 to 25 percent of bacillus cereus, 5 to 10 percent of bacillus smith, 5 to 10 percent of geobacillus thermodenitrificans, 20 to 40 percent of bacillus licheniformis, 5 to 24 percent of ureibacillus thermophilus and the balance of actinomyces thermophilus; the oxygen content is controlled to be 5-15%; the 50-60 DEG C high-temperature period is maintained for 180-210 days, the heat exchange pipe located between the two layers of fermentation raw materials is connected to a heating system in the high-temperature period, and the system is suitable for agricultural circular economy and distributed energy supply scenes.

The invention relates to the technical field of microbial fertilizers. The invention aims to provide a fruit and vegetable biological source bacterial fertilizer system which comprises the following raw materials in parts by weight: 600 parts of wormcast, 200 parts of straw powder, 100 parts of humic acid, 1 part of a microbial agent and 5 parts of brown sugar. The microbial agent is prepared from bacillus subtilis, bacillus megatherium and actinomycetes according to the weight ratio of 5: 3: 2. According to the invention, the natural wormcast is used as a microbial carrier, and by virtue of the unique physical structure and microbial basis of the wormcast and in combination with a functional strain compounding technology, the defects of the traditional fertilizer can be effectively overcome.

To provide a system including novel nanopores having different structures and recognition sites that improve sequencing accuracy and / or provide different error profiles.SOLUTION: Provided are systems relating to the field of nanopores and their use in various applications, e.g., analysis of biopolymers and macromolecules, typically by making electrical measurements during translocation through the nanopore, comprising a funnel-shaped proteinaceous nanopore comprising alpha-helical pore-forming toxins that are members from the actinoporin family of proteins, more particularly Fraga Ceatoxin C (FraC), a variant FraC, a FraC paralog or a FraC homolog.SELECTED DRAWING: None

The present application relates to the technical field of eutrophication water treatment, and discloses a preparation method and application of active substances of algicidal actinomycetes, which comprises the following steps: (1) inoculating actinomycetes into a shaking flask containing a culture medium, wherein the culture medium comprises carbohydrates, salts, glutamic acid and cyanobacteria, and then placing the shaking flask in a culture box for oscillation culture to obtain a first-stage seed liquid; (2) inoculating the first-stage seed liquid into a first-stage seed tank according to a volume fraction of 10-20% to obtain a second-stage seed liquid; (3) inoculating the second-stage seed liquid into a fermentation tank according to a volume fraction of 10-20% to obtain an actinomycete fermentation liquid; and (4) centrifuging the actinomycete fermentation liquid to obtain a supernatant and mixing the supernatant with a stabilizer. Glutamic acid can promote the actinomycetes to secrete more active substances during the fermentation process, and cyanobacteria can enable the actinomycetes to maintain the ability to remove algae during the fermentation process to secrete corresponding active substances. The stabilizer can inhibit the growth of miscellaneous bacteria to maintain the stability of algae removal.

The present invention provides a prophylactic or therapeutic composition for graft-versus-host disease (GVHD). There is provided a prophylactic or therapeutic composition for GVHD, which comprises bacteria belonging to any genus selected from the group consisting of the following genera: Blautia, Clostridium, unclassified Clostridiales, Actinomyces, Parabacteroides, Lachnoclostridium, Bacteroides, Faecalibacterium, unclassified Lachnospiraceae, Roseburia, Ruminococcus, unclassified Firmicutes, Dorea, Phascolarctobacterium, Sutterella, Megamonas, Collinsella, Eubacterium, and Coprococcus, etc., or any combination of bacteria belonging to these genera.

The invention relates to an actinomycete preparation and application thereof. The actinomycete preparation is a product obtained by culturing actinomycetes with insect fecula. When the actinomycetes preparation is stored for five months, half a year or one year or more, the content of the actinomycetes in the actinomycetes preparation is 1 * 10 < 11 > CFU / g or more.

The invention discloses a naphthocycline type pentacyclic aromatic polyketone compound. The structural formula of the naphthocycline type pentacyclic aromatic polyketone compound is as shown in a formula (I): # imgabs0 #. According to the invention, six naphthocycline type pentacyclic aromatic polyketone compounds are separated from a rice fermentation product of actinomycetes Streptomyces sp.NAK5495 for the first time, and the six naphthocycline type pentacyclic aromatic polyketone compounds are separated from rice fermentation products of actinomycetes Streptomyces sp.NAK5495; according to the present invention, with the medium-pressure liquid chromatography (MPLC) system and the semi-preparative HPLC separation and purification technology, the efficient separation and purification of the naphthocycline pentacyclic aromatic polyketones are achieved, and the recovery rate and the purity of the target compound are improved.

The invention belongs to the technical field of soil conditioners and fertilizers, and particularly relates to a bio-organic fertilizer for improving salt and alkali as well as a preparation method and application of the bio-organic fertilizer. The method comprises the following preparation steps: (1) carrying out pyrolysis treatment on dry agricultural and forestry wastes or livestock and poultry manure under anaerobic and 400-700 DEG C conditions, and crushing to obtain a biochar material; and (2) mixing and grinding the obtained biochar material, soybean meal and water, and then inoculating a bacillus subtilis activation solution and an actinomycetes activation solution for fermentation treatment to obtain the saline-alkaline improved bio-organic fertilizer. According to the bio-organic fertilizer, the biochar prepared from agricultural and forestry waste or animal dung is used as a main raw material, EPS generated through in-situ loading microbial fermentation metabolism can synergistically improve the improvement effect of the obtained organic fertilizer on saline-alkali soil and the passivation effect on heavy metal, absorption of crops on the heavy metal is remarkably reduced, and the fertilizer efficiency is improved.

The invention belongs to the technical field of soil improvement, particularly relates to a compound microorganism saline-alkali soil conditioner as well as a preparation method and application thereof, and aims at solving the problems that the existing soil conditioner mostly adopts a single strain or a simple combination, is difficult to colonize in a harsh saline-alkali environment, has a single function, cannot systematically improve the physical and chemical properties of soil, is slow in organic material decomposition and is difficult to promote. In order to solve the problems that in the prior art, the soil conditioner is not durable in effect due to the fact that the soil conditioner is prone to failure due to saline-alkali stress, the following scheme is provided: the soil conditioner comprises the following raw materials in parts by weight: 5-10 parts of bacillus; by using bacillus, streptomyces, saccharomycetes, actinomycetes, nitrogen-fixing bacteria, phosphate bacteria, potassium bacteria, pyroligneous liquor, seaweed extract, amino acid chelate, potassium fulvate, citric acid and monopotassium phosphate, the content of organic matters in soil can be effectively increased, the aggregate structure of the soil is improved, a more lasting soil improvement effect is achieved, and the soil conditioner has a good application prospect. And the use frequency and cost of the modifier are reduced.

The utility model belongs to the technical field of gradient dilution pipe frames, and discloses a gradient dilution pipe frame for separating actinomycetes in rhizosphere soil of ginseng, a column groove is formed in the middle of the interior of a gradient dilution disc pipe frame, a rotating assembly is fixedly arranged in the column groove, and the rotating assembly comprises a hollow circular ring; the test tubes with different dilution gradients can be flexibly rotated to a position convenient to operate by utilizing the rotating assembly, so that an operator does not need to frequently move or change the position when sucking diluents with different gradients, the operation convenience and smoothness are remarkably improved, personal errors are reduced, and the operation efficiency is improved. More test tubes can be orderly arranged in a limited space, so that the space utilization rate is increased, the separation operation efficiency is accelerated, meanwhile, solutions in the tubes are further uniformly mixed in the rotating process, and the separation effect is improved.

The invention discloses a virus-free culture method of lily bulbs, and relates to the technical field of lily bulb breeding, stem tips are adopted to culture lily bulbs, the stem tips have no vascular bundles, so that the lily bulbs contain few viruses, and an antioxidant is added to inhibit browning during stem tip culture; the preparation method comprises the following steps: preparing an antioxidant microcapsule by taking polyvinylpyrrolidone as a capsule wall and vitamin C, tert-butylhydroquinone and butylated hydroxyanisole as a capsule core, loading the antioxidant microcapsule on the surface of activated carbon, and adding a culture medium, so that the browning phenomenon is effectively inhibited; according to the method, detoxification propagation is carried out by utilizing a heat treatment method, the detoxification rate is effectively improved, detoxification is carried out by utilizing chemical treatment, and virus infection can be effectively inhibited by adding virazole, actinomycin D and 5-dihydrouracil into a culture medium, so that detoxified lily bulbs are cultured.

The invention belongs to the field of microbial agents, and particularly relates to a method for regulating and controlling compost heat production of waste agaric fungus bags in a layered manner by using a complex microbial agent, which comprises the following steps: mixing crushed waste agaric fungus bags with chicken manure to obtain a fermentation raw material; the fermentation raw materials are tiled in a fermentation container in a layered mode, after each layer of fermentation raw materials is tiled, the water content is adjusted, complex microbial inoculants are evenly sprayed on the surface of each layer of fermentation raw materials, the first layer of fermentation raw materials at the bottom is provided with ureabacillus thermosphaeroides, ureabacillus thermosphaeroides and clostridium thermophile, and the second layer of fermentation raw materials at the bottom is provided with actinomycetes and lactic acid bacteria; bacillus subtilis, bacillus licheniformis and bacillus cereus are arranged on the third layer of fermentation raw materials at the bottom; saccharomycetes are arranged on the fourth layer of fermentation raw materials; and in the high-temperature period, the device is connected with a heating system through a heat exchange pipe pre-buried between the two layers of fermentation raw materials, and the device is suitable for efficient degradation and heat energy recovery of the agaric fungus bags.

The application discloses a method for improving information storage capacity of actinomycete, and belongs to the technical field of genetic engineering. The method comprises the steps of simulating acetylation mutation or simulating deacetylation mutation of DasR protein of the actinomycete; the simulating acetylation mutation is that lysine at the 78th position of the DasR protein is mutated into glutamine; and the simulating deacetylation mutation is that lysine at the 78th position of the DasR protein is mutated into arginine. Through acetylation mutation and deacetylation mutation of specific sites of the wide-range regulatory factor protein, the growth of the improved Actinomucor rubrum is determined, and the sporulation capacity of the strain is observed, so that it is confirmed that the optimization of the spore growth capacity and growth condition of the Actinomucor rubrum can be realized by using genetic engineering modification, new microbial resources are provided for preparation of an information storage carrier, and a research foundation is provided for promotion of a DNA data storage technology.

The present application provides a kind of compost and its preparation method, it is related to the technical field of organic fertilizer, compost includes the following weight parts of component: crop straw 900-1100 parts, animal excrement 200-300 parts, organic auxiliary material 100-200 parts, compound microbial inoculum 1.5-7.5 parts;The compound microbial inoculum includes lactic acid bacteria, bacillus licheniformis, thermophilic filamentous bacteria, actinomycetes, white rot fungus.The preparation method of compost mainly is: crop straw and organic auxiliary material are stacked, and solidified layer by layer;After evenly spattering animal excrement on each layer, inoculate compound microbial inoculum again;Hole is punched in the top of pile to monitor the moisture content in the pile in the process of composting;Composting process is carried out by turning over treatment.The composting treatment of the present application is carried out by using compound microbial inoculum, not only can speed up the composting speed, can also improve composting effect, to improve the nutrient content in compost.

The invention discloses a microbial fertilizer for treating citrus huanglongbing and a preparation method of the microbial fertilizer, and belongs to the field of agricultural biological control. 33 to 50 percent of sodium alginate-chitosan microspheres; 5 to 8% of hesperidin; 3-5% of nano zinc oxide; 2-4% of humic acid chelated boron; 2-4% of sucrose fatty acid ester; 2-4% of diatomite; wherein the preparation method of the sodium alginate-chitosan microspheres comprises the following steps: mixing sodium alginate with a microbial suspension, dropwise adding the mixture into a calcium chloride solution by using an injector, and standing and curing to form a microsphere core; and immersing the cured microspheres into a chitosan solution with the pH value of 6.0, taking out, and freeze-drying to obtain finished microspheres. Wherein the microbial suspension comprises trichoderma, bacillus and actinomycetes. The invention solves the problems of poor prevention and treatment effect of the existing Candidatus Liberobacter asiaticum, difficulty in colonization of the strain at phloem, short survival time and lack of targeted slow release function.

The invention relates to a method for degrading petroleum hydrocarbon in soil by utilizing goethite reinforced endogenous microorganisms, goethite is used as a biological stimulant to activate the activity of soil endogenous functional bacteria for degrading petroleum hydrocarbon, dominant phylum of the soil endogenous functional bacteria is proteobacteria, actinomycetes and thick-wall phylum, and the relative abundance accounts for 50-80%. Under the conditions that the pH value of soil is 6.0-9.0 and the water content of the soil is 20%-50%, the degradation rate of total petroleum hydrocarbon in the soil is larger than or equal to 60%, and the degradation rate of components such as colloid and asphaltene is larger than or equal to 45%.

The invention discloses a macadamia nut fruit retention and fruit setting fertilizer based on hormone synergistic regulation and an application method thereof, and relates to the technical field of fertilizers. The fertilizer comprises a first component, a second component, a third component and a fourth component, the first component is prepared from the following raw materials: urea, vermiculite, water-retaining composite gel, humic acid, pseudomonas fluorescens, actinomycetes and decomposed straw; the second component raw materials comprise stimulant, gibberellin, auxin, sodium thiosulfate, mineral oil, polyvinyl alcohol, calcium chloride, potassium silicate, boric acid and brassinosteroid; a third component raw material: a medium-nitrogen low-phosphorus high-potassium compound fertilizer and a medium-trace element fertilizer; and the fourth component comprises the following raw materials: compound sodium nitrophenolate, isopentenyl adenine, glycine betaine, mineral oil and polyvinyl alcohol. The fertilizer disclosed by the invention can provide sufficient water and nutrients in a young fruit stage, reduces the physiological fruit dropping rate in the early stage and the middle and later stages, can promote protein and fat accumulation in a fruit expansion stage and a fruit maturation stage, and reduces the physiological fruit dropping rate in the middle and later stages.

The present invention relates to an actinomycete preparation and the use thereof. The actinomycete preparation is a product obtained by culturing actinomycetes with insect frass. Upon storage for a period of five months, six months, or one year or longer, the content of the actinomycetes in the actinomycete preparation is 1×1011 CFU / g or more.

The invention belongs to the technical field of microbial agents, and particularly relates to a special microbial agent for edible mushroom mixing and a preparation method thereof, the special microbial agent comprises composite functional strains of bacillus subtilis, bacillus licheniformis, bacillus megatherium, aspergillus oryzae and actinomycetes, and the microbial agent is prepared by liquid fermentation propagation, adsorption on a solid carrier and drying. According to the invention, after three-stage liquid fermentation and propagation, the bacterial agent is adsorbed on a grass meal carrier and is air-dried at low temperature, the bacterial agent can be simultaneously adapted to various edible fungus cultivation modes such as fermented materials, semi-cooked materials and cooked materials, after material mixing, the strain is fast to revive, the temperature rise is fast, the high-temperature duration time is long, infectious microbes can be effectively killed, lignocellulose can be decomposed, and the compost can be promoted to be fully decomposed; and the product is a pure microbial preparation, does not contain chemical agents, does not have the risk of harmful substance residues, ensures the food safety and quality of the edible mushrooms, and has ecological benefits as the beneficial flora can construct a dominant microbial environment in the culture material.

The invention provides a strain of halotolerant bacteria of Kocuria (Kocuria) and application of the halotolerant bacteria of Kocuria to growth promotion of corn and wheat under the stress of salt, and particularly provides a strain of halotolerant bacteria of Kocuria (Kocuria) and application of the halotolerant bacteria of Kocuria (Kocuria) to growth promotion of corn and wheat under the stress of salt. The salt-tolerant actinomycete JC is separated from drought saline-alkali soil in Longzhong Gansu Province, grows well in an LB culture medium with the pH value of 8 and containing 30 g / L of NaCl, and is preserved in the China General Microbiological Culture Collection Center on March 17, 2025, and the preservation number is CGMCC No. 33847. The strain has multiple functions of resisting salt and alkali, reducing salt and alkali, producing IAA, fixing nitrogen, dissolving inorganic phosphorus, promoting growth of corn and wheat seedlings and the like. Experiments prove that the strain can reduce salt and alkali by 7.54% and 6.55%, and can significantly relieve saline-alkali stress of crops and promote growth. The method has important application value in the aspects of improving the production capacity of plants in a saline-alkali environment, improving saline-alkali soil and the like.

The invention discloses a microbial flora for treating sulfate wastewater and a preparation method of the microbial flora. Comprising the following microbial treatment flora components: 10-35% of biphasic photosynthetic bacteria, 5-10% of sulfate reducing bacteria, 5-30% of bifidobacterium, 3-8% of desulfurization vibrio, 5-25% of mesophilic actinomycetes, 2-5% of methanogens, 10-30% of sub-digestion bacteria, 2-6% of acidithiobacillus acidophilus, 12-30% of bacillus megatherium, 1-4% of iron-oxidizing bacteria, 1-5% of denitrifying bacteria and 1-3% of phosphorus-accumulating bacteria. According to the method, sulfate is efficiently removed, the removal rate of SO4 < 2-> is larger than or equal to 85%, the H2S output is reduced by 70% or above, multi-pollutant cooperative treatment of odor pollution is avoided, the removal rate of COD is larger than or equal to 60%, the removal rates of total nitrogen and total phosphorus are larger than or equal to 50% and 40% respectively, the comprehensive discharge standard is met, the environmental adaptability is high, the salt tolerance NaCl is smaller than or equal to 5%, the temperature fluctuation resistance is 20-40 DEG C, and the method is suitable for the complex environment of industrial wastewater, low in cost and suitable for industrial production. Extra chemical agents are not needed, strains can be bred autonomously, and the operation cost is reduced by 40% or above compared with a traditional method.

The invention discloses a microbial agent for in-situ treatment of enteromorpha in an open sea area as well as a preparation method and application of the microbial agent. The microbial agent is naturally formed by fermentation of a composite flora under a proper condition, and the pH value of the microbial agent is less than 4.0; the composite flora comprises bacillus subtilis, nitrifying bacteria, actinomycetes, filamentous bacteria, photosynthetic bacteria, lactobacillus plantarum, saccharomyces cerevisiae, candida, lactobacillus paracasei and bacillus megatherium, and can be applied to differential in-situ treatment of enteromorpha in different scenes (beaches, sea floats and cabins) in open sea areas. The microbial agent is an ecological treatment microbial agent, an acidity chemical regulator is not added, the enteromorpha prolifera in the open sea area can be treated in situ in a strong mode within a short time, and no negative influence is caused to seawater and the marine environment.