122 results about "Actinomyces" patented technology

The invention discloses a high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation. According to the high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation, yield increase and quality improvement are achieved through fungus material composite strengthening treatment and a composite strain synergistic inoculation technology. The method comprises the following steps: (1) fermenting a tilia brachypoda substrate by lactobacillus plantarum and soaking in a nano-composite nutrient solution to form a high-activity bacterial material; (2) nitrogen-fixing bacteria, disease-resistant actinomycetes and armillaria mellea are mixed and inoculated according to the proportion of 3: 1: 1, nitrogen supply is improved, and the occurrence rate of black rot is reduced; 3) activating a GASA stress-resistant gene of the seed hemp through a pulsed electric field (5 kV / cm, 10 microseconds), and enabling the germination rate to reach 88.5% in combination with a calcium signal channel strengthening technology; according to the method, the yield per mu of gastrodia elata is increased by 30%-40%, the gastrodin content is increased by 30% or above, the chemical pesticide dosage is reduced by 55%, the strain passage purity is stable, the method is suitable for large-scale planting in multiple climate areas, and an innovative solution is provided for green production of traditional Chinese medicinal materials.

The invention discloses a bifidobacterium animalis subsp. Lactis BA-C53 and an application of the bifidobacterium animalis subsp. Lactis BA-C53 in products for improving bone health. The bifidobacterium animalis subsp. Lactis BA-C53 is classified and named as bifidobacterium animalis subsp. Lactis Bifidobacterium animalis subsp. Lactis BA-C53, the preservation number is CCTCC NO: M 2024460, the preservation unit is China Center for Type Culture Collection, and the preservation time is March 11, 2024. The bifidobacterium animalis subsp. Lactis BA-C53 can effectively improve the steady state of intestinal flora of a mouse with osteoporosis induced by estrogen deficiency, so that the relative abundance of phylum firmicalis and phylum actinomycetes is increased, the relative abundance of phylum bacteroides is reduced, and then short-chain fatty acid is generated to activate intestinal immunity and maintain the integrity of an intestinal barrier, so that the osteoporosis caused by estrogen deficiency is inhibited. Meanwhile, osteoclast differentiation can be inhibited by regulating NFATC1, RANKL and OPG pathways, then osteoporosis is relieved, and the compound can be widely applied to preparation of food and drugs.

To provide a system including novel nanopores having different structures and recognition sites that improve sequencing accuracy and / or provide different error profiles.SOLUTION: Provided are systems relating to the field of nanopores and their use in various applications, e.g., analysis of biopolymers and macromolecules, typically by making electrical measurements during translocation through the nanopore, comprising a funnel-shaped proteinaceous nanopore comprising alpha-helical pore-forming toxins that are members from the actinoporin family of proteins, more particularly Fraga Ceatoxin C (FraC), a variant FraC, a FraC paralog or a FraC homolog.SELECTED DRAWING: None

The invention relates to an actinomycete preparation and application thereof. The actinomycete preparation is a product obtained by culturing actinomycetes with insect fecula. When the actinomycetes preparation is stored for five months, half a year or one year or more, the content of the actinomycetes in the actinomycetes preparation is 1 * 10 < 11 > CFU / g or more.

The invention belongs to the technical field of soil conditioners and fertilizers, and particularly relates to a bio-organic fertilizer for improving salt and alkali as well as a preparation method and application of the bio-organic fertilizer. The method comprises the following preparation steps: (1) carrying out pyrolysis treatment on dry agricultural and forestry wastes or livestock and poultry manure under anaerobic and 400-700 DEG C conditions, and crushing to obtain a biochar material; and (2) mixing and grinding the obtained biochar material, soybean meal and water, and then inoculating a bacillus subtilis activation solution and an actinomycetes activation solution for fermentation treatment to obtain the saline-alkaline improved bio-organic fertilizer. According to the bio-organic fertilizer, the biochar prepared from agricultural and forestry waste or animal dung is used as a main raw material, EPS generated through in-situ loading microbial fermentation metabolism can synergistically improve the improvement effect of the obtained organic fertilizer on saline-alkali soil and the passivation effect on heavy metal, absorption of crops on the heavy metal is remarkably reduced, and the fertilizer efficiency is improved.

The invention belongs to the technical field of soil improvement, particularly relates to a compound microorganism saline-alkali soil conditioner as well as a preparation method and application thereof, and aims at solving the problems that the existing soil conditioner mostly adopts a single strain or a simple combination, is difficult to colonize in a harsh saline-alkali environment, has a single function, cannot systematically improve the physical and chemical properties of soil, is slow in organic material decomposition and is difficult to promote. In order to solve the problems that in the prior art, the soil conditioner is not durable in effect due to the fact that the soil conditioner is prone to failure due to saline-alkali stress, the following scheme is provided: the soil conditioner comprises the following raw materials in parts by weight: 5-10 parts of bacillus; by using bacillus, streptomyces, saccharomycetes, actinomycetes, nitrogen-fixing bacteria, phosphate bacteria, potassium bacteria, pyroligneous liquor, seaweed extract, amino acid chelate, potassium fulvate, citric acid and monopotassium phosphate, the content of organic matters in soil can be effectively increased, the aggregate structure of the soil is improved, a more lasting soil improvement effect is achieved, and the soil conditioner has a good application prospect. And the use frequency and cost of the modifier are reduced.

The utility model belongs to the technical field of gradient dilution pipe frames, and discloses a gradient dilution pipe frame for separating actinomycetes in rhizosphere soil of ginseng, a column groove is formed in the middle of the interior of a gradient dilution disc pipe frame, a rotating assembly is fixedly arranged in the column groove, and the rotating assembly comprises a hollow circular ring; the test tubes with different dilution gradients can be flexibly rotated to a position convenient to operate by utilizing the rotating assembly, so that an operator does not need to frequently move or change the position when sucking diluents with different gradients, the operation convenience and smoothness are remarkably improved, personal errors are reduced, and the operation efficiency is improved. More test tubes can be orderly arranged in a limited space, so that the space utilization rate is increased, the separation operation efficiency is accelerated, meanwhile, solutions in the tubes are further uniformly mixed in the rotating process, and the separation effect is improved.

The application discloses a method for improving information storage capacity of actinomycete, and belongs to the technical field of genetic engineering. The method comprises the steps of simulating acetylation mutation or simulating deacetylation mutation of DasR protein of the actinomycete; the simulating acetylation mutation is that lysine at the 78th position of the DasR protein is mutated into glutamine; and the simulating deacetylation mutation is that lysine at the 78th position of the DasR protein is mutated into arginine. Through acetylation mutation and deacetylation mutation of specific sites of the wide-range regulatory factor protein, the growth of the improved Actinomucor rubrum is determined, and the sporulation capacity of the strain is observed, so that it is confirmed that the optimization of the spore growth capacity and growth condition of the Actinomucor rubrum can be realized by using genetic engineering modification, new microbial resources are provided for preparation of an information storage carrier, and a research foundation is provided for promotion of a DNA data storage technology.

The present application provides a kind of compost and its preparation method, it is related to the technical field of organic fertilizer, compost includes the following weight parts of component: crop straw 900-1100 parts, animal excrement 200-300 parts, organic auxiliary material 100-200 parts, compound microbial inoculum 1.5-7.5 parts;The compound microbial inoculum includes lactic acid bacteria, bacillus licheniformis, thermophilic filamentous bacteria, actinomycetes, white rot fungus.The preparation method of compost mainly is: crop straw and organic auxiliary material are stacked, and solidified layer by layer;After evenly spattering animal excrement on each layer, inoculate compound microbial inoculum again;Hole is punched in the top of pile to monitor the moisture content in the pile in the process of composting;Composting process is carried out by turning over treatment.The composting treatment of the present application is carried out by using compound microbial inoculum, not only can speed up the composting speed, can also improve composting effect, to improve the nutrient content in compost.

The invention relates to a method for degrading petroleum hydrocarbon in soil by utilizing goethite reinforced endogenous microorganisms, goethite is used as a biological stimulant to activate the activity of soil endogenous functional bacteria for degrading petroleum hydrocarbon, dominant phylum of the soil endogenous functional bacteria is proteobacteria, actinomycetes and thick-wall phylum, and the relative abundance accounts for 50-80%. Under the conditions that the pH value of soil is 6.0-9.0 and the water content of the soil is 20%-50%, the degradation rate of total petroleum hydrocarbon in the soil is larger than or equal to 60%, and the degradation rate of components such as colloid and asphaltene is larger than or equal to 45%.

The present invention relates to an actinomycete preparation and the use thereof. The actinomycete preparation is a product obtained by culturing actinomycetes with insect frass. Upon storage for a period of five months, six months, or one year or longer, the content of the actinomycetes in the actinomycete preparation is 1×1011 CFU / g or more.

The invention belongs to the technical field of microbial agents, and particularly relates to a special microbial agent for edible mushroom mixing and a preparation method thereof, the special microbial agent comprises composite functional strains of bacillus subtilis, bacillus licheniformis, bacillus megatherium, aspergillus oryzae and actinomycetes, and the microbial agent is prepared by liquid fermentation propagation, adsorption on a solid carrier and drying. According to the invention, after three-stage liquid fermentation and propagation, the bacterial agent is adsorbed on a grass meal carrier and is air-dried at low temperature, the bacterial agent can be simultaneously adapted to various edible fungus cultivation modes such as fermented materials, semi-cooked materials and cooked materials, after material mixing, the strain is fast to revive, the temperature rise is fast, the high-temperature duration time is long, infectious microbes can be effectively killed, lignocellulose can be decomposed, and the compost can be promoted to be fully decomposed; and the product is a pure microbial preparation, does not contain chemical agents, does not have the risk of harmful substance residues, ensures the food safety and quality of the edible mushrooms, and has ecological benefits as the beneficial flora can construct a dominant microbial environment in the culture material.

The invention relates to the technical field of fertilizers, and discloses a biological protein principle-based fertilizer for preventing and treating citrus liberobacter asiaticum, which is prepared from the following raw materials in parts by weight: 40 to 70 parts of fish protein peptide fermentation products, 5 to 15 parts of EM flora active liquid, 1 to 5 parts of chelated trace element solution and 10 to 20 parts of algal polysaccharide-polyglutamic acid compound. The fish protein peptide is fermented for 180 days or longer through EM bacteria. The EM flora active liquid contains lactic acid bacteria, photosynthetic bacteria, actinomycetes and saccharomycetes. The trace elements are at least two of boron, zinc, iron, manganese and molybdenum and are chelated by polyglutamic acid or sugar alcohol. The small peptide, the bacterial metabolite and the chelate element are in slow release synergy, so that rhizosphere microecology can be rebuilt, defensive enzymes can be induced, and the phenomena of huanglong, yellowing and red nose can be relieved.

The invention belongs to the technical field of biological agents, and particularly relates to composite IgY antibody freeze-dried powder for pet oral hygiene and a preparation method and application thereof. Oral bacteria such as prevotella intermedia, porphyromonas gingivalis, streptococcus mutans, actinomyces viscosus and fusobacterium nucleatum are selected in a targeted manner, the bacteria are used for preparing a composite IgY antibody, the composite IgY antibody is combined with the freeze-drying agent optimized by the invention, and the antibody freeze-dried powder is prepared by immunizing laying hens, so that the range of the bacteria is wider, and the immunogenicity of the laying hens is improved. The compound IgY antibody obtained by the prepared compound IgY antibody freeze-dried powder is high in activity, and the compound IgY antibody freeze-dried powder has good re-dissolvability, stability, affordability, simplicity and convenience, can maintain titer at-20 DEG C for more than two years, and is convenient to store, transport and popularize. Compared with the prior art, the composite IgY antibody freeze-dried powder aims at wider flora, can effectively resist canine oral malignant bacteria, and can be used as an additive to be added into oral health products such as toothpaste and mouth wash.

The application discloses application of marine actinomycetes of Saccharothrix sp. in improving heat resistance of algae and / or promoting growth of algae, and relates to the technical field of microbial mutation breeding. The marine actinomycetes of Saccharothrix sp. is screened by using a method of ultraviolet mutation. Compared with a wild type, the bacteria can obviously improve the high-temperature resistance of algae, and has the characteristics of promoting the growth of algae. It is indicated that the bacteria strain has good application prospect of improving the heat resistance of algae and / or promoting the growth of algae.

The application discloses a kind of ultra-high temperature aerobic fermentation composite microbial inoculum for treating organic solid waste, belonging to solid waste biological treatment technical field.The composite microbial inoculum is compounded by functional microbial inoculum group and auxiliary group, the functional microbial inoculum group includes thermophilic side spore mold agent, thermophilic bacillus agent, high-temperature actinomycete agent, fecal alkali-producing bacteria agent, the auxiliary group is composed of modified diatomite, coated fungus bran carbon, oyster shell calcined powder, kitchen waste pretreatment powder, sodium lignosulfonate and sodium gluconate.The composite microbial inoculum is adapted to municipal sludge, livestock and poultry manure, open-air strip-pile type fermentation of kitchen waste mixed solid waste, can be quickly heated to 80 DEG C or more, ultra-high temperature duration is greater than or equal to 7.8 days, roundworm egg inactivation rate is greater than or equal to 99.9%, comprehensive antibiotic degradation rate is greater than or equal to 90.2%, heavy metal comprehensive passivation rate is greater than or equal to 85.5%, effectively control odor emission, can be applied to municipal sludge, livestock and poultry manure, open-air strip-pile type fermentation of kitchen waste mixed solid waste, and has good industrial application value.

The invention discloses a photovoltaic pond river crab culture method, which comprises the following steps: a bacteria-algae compound conditioning agent is put into a target photovoltaic pond according to a preset period and a preset putting dosage, the bacteria-algae compound conditioning agent comprises an effective microbial flora microbial agent and a chlorella complex which are mixed according to a preset proportion, the pH value of the effective microbial flora microbial agent is 3.0-4.0, the pH value of the chlorella complex is 3.0-4.0, and the pH value of the chlorella complex is 3.0-4.0. The content of lactic acid bacteria in the effective microbial agent is 1.0 * 10 to 1.0 * 10 CFU / mL, the content of saccharomycetes in the effective microbial agent is 1.0 * 10 to 1.0 * 10 CFU / mL, the content of photosynthetic bacteria in the effective microbial agent is 1.0 * 10 to 2.0 * 10 < 3 > CFU / mL, the content of actinomycetes in the effective microbial agent is 1.0 * 10 < 3 > to 3.0 * 10 < 3 > CFU / mL, and the concentration of chlorella in the chlorella complex is 1.0 * 10 cells / mL; and monitoring and dynamically adjusting the water quality in the target photovoltaic pond, and adjusting the putting frequency and the putting dosage of the bacteria-algae compound regulator within a preset range according to a monitoring result so as to maintain or restore the ecological balance of the water body and the river crabs. According to the technical scheme, the problem of micro-ecological unbalance of photovoltaic shading is solved, oxygen is supplied by algae, diseases are inhibited by bacteria, and carbon sequestration by bacteria and algae is realized, so that the breeding survival rate is increased, and the growth of river crabs is promoted.

The invention discloses a microorganism for treating moss and foul smell in park landscape lake water and a culture process thereof, the microorganism comprises biphase photosynthetic bacteria, decomposing bacteria and a culture medium, the decomposing bacteria comprise bifidobacterium, mesophilic actinomycetes, nitrosobacteria and bacillus megatherium, the weight percentage of the decomposing bacteria is 1-5%, and the weight percentage of the decomposing bacteria is 1-5%. The microbial inoculant comprises the following components in percentage by weight: 20-30% of biphasic photosynthetic bacteria, 15-25% of bifidobacterium, 10-20% of mesophilic actinomycetes, 10-20% of nitrosobacteria and 15-25% of bacillus megatherium. The biphase photosynthetic bacteria and the composite decomposition bacteria are scientifically proportioned to form a microbial system with complementary functions, the biphase photosynthetic bacteria can directionally inhibit photosynthesis of moss, destroy cell structures of moss and realize targeted killing, and the decomposition bacteria synergistically decompose organic matters such as underwater residual feed, excrement, animal and plant bodies and the like, so that the biological activity of moss is improved. Meanwhile, nitrosobacteria can convert algal toxins into harmless substances, the synergistic effect overcomes the defects that an existing single microbial preparation is single in function and incomplete in treatment, and integrated treatment of killing moss, eliminating stink and improving water quality is achieved.

The invention discloses an Actinomyces sp. Strain, a separation and purification method and application of the Actinomyces sp. Strain in straw degradation, the Actinomyces sp. Strain is Actinomyces sp. And has been preserved in the China General Microbiological Culture Collection Center on July 3, 2025, and the preservation number of the Actinomyces sp. Strain is CGMCC No. 46460. As facultative anaerobic bacteria, the Actinomycete disclosed by the invention can be used for realizing efficient degradation under an anaerobic or aerobic condition in a single system, so that the process is remarkably simplified. In the practical application process, the strain can be used as a functional strain to be added into straw compost, organic matter decomposition is promoted, the composting period is shortened, and meanwhile the humus content is increased; fermentable sugar is generated by pretreating straws and is used for producing bioethanol or biogas; and the degraded straw residues can enhance the water-retaining property and microbial activity of the soil.

The invention belongs to the technical field of microorganisms and novel medicine and veterinary drugs, particularly relates to a marine micromonospora-sourced nitrogen-chlorine indole compound, and further discloses a preparation method and application thereof. According to the invention, two indole compounds containing nitrogen and chlorine groups and having antiviral potential are separated and screened from the preserved marine actinomycete micromonospora sp. FIMYZ52, so that the compound is provided for research and development of new antiviral drugs, and has important value for development and utilization of marine drug resources in China.

The invention discloses a biological control method for cotton fusarium wilt. The invention provides a combined bacterium or a complex microbial inoculant containing the combined bacterium. The combined bacterium is prepared from AT actinoplanes (Actinoplanes) Q9316 (CGMCC (China General Microbiological Culture Collection Center) No.35443) and Trichoderma asperellum (Trichoderma asperellum) F2609 (CGMCC No.42159). The invention further provides a preparation method of the composite microbial inoculant. The fermentation liquid independently fermented by the trichoderma asperellum F2609 and the AT actinoplanes Q9316 provided by the invention has an inhibition effect on pathogenic bacteria of cotton fusarium wilt, and the bacteriostatic activity strength of the fermentation liquid jointly fermented by the trichoderma asperellum F2609 and the AT actinoplanes Q9316 exceeds that of the fermentation liquid fermented by single bacteria. The invention has important significance for biological prevention and control of cotton fusarium wilt.

The invention belongs to the technical field of microorganisms, and particularly relates to a mangrove forest actinomycete secondary metabolite extraction device which comprises a bottom plate, a supporting plate is fixed to the rear end of the upper side of the bottom plate, and a fixing box is fixed to the front side of the supporting plate. A water phase at the lower end can be discharged through a first discharge pipe, and after extract liquor collected for the first time in the extraction pipe is poured out, the water phase flows back to the extraction pipe again, so that an ethanol solution is added again for extraction, and the extraction effect of mangrove forest actinomycetes secondary metabolites is improved; and after the extraction process is finished, a cleaning water containing box at the upper end can be driven to move through a moving mechanism, so that cleaning water is injected into the extraction pipe through a second vertical pipe, and the cleaning water in the extraction pipe circularly flows through the work of a backflow mechanism and a first pump body, so that the cleaning effect is achieved.

The invention provides amycolatopsis mediterranei with gene defects as well as a construction method and application of the amycolatopsis mediterranei. The first aspect of the invention provides a construction method of the amycolatopsis mediterranei with the gene defect, and the construction method comprises the step of knocking out a rifamycin biosynthetic gene cluster in a receptor amycolatopsis mediterranei strain U32 (Cas12a) genome and an integrated plasmid pDZLCas12a containing an FnCas12a coding gene to obtain the amycolatopsis mediterranei with the gene defect. According to the invention, a rifamycin biosynthetic gene cluster in a genome of an amycolatopsis mediterranei strain U32 (Cas12a) and an integrated plasmid pDZLCas12a containing an FnCas12a coding gene are knocked out, so that the amycolatopsis mediterranei with gene defects is constructed, and the amycolatopsis mediterranei can be used as a chassis strain for mining identified or unidentified biosynthetic gene clusters in actinomycetes.

The invention discloses an intestinal microbial marker combination for noninvasive diagnosis of intrahepatic cholestasis and application of the intestinal microbial marker combination, and belongs to the technical field of medical treatment. According to the invention, by integrating metagenome sequencing and a machine learning algorithm, a group of intestinal microbial marker combination composed of 12 bacterial species is screened; the bacillus subtilis specifically comprises atypical virenoccus virens, Wilbionan isonibacterium, Brawtella, mucophilic protein anaerobic corynebacterium, bacteroides simplex, a clostridium AT4 strain, sargassum purpurascens, lawsonia sacchariflora, clostridium enteric, actinomycetes grignard, brassicaceae marsi Q3528 and bile vowenii. The AUC of a diagnosis model constructed based on the marker combination on an independent test set reaches 0.871, and the sensitivity and the specificity reach 86.7% and 81.3% respectively. The invention provides a non-invasive, simple, convenient and accurate auxiliary diagnosis tool for intrahepatic cholestasis, and has excellent clinical transformation prospects.

The present application relates to the technical field of inorganic fiber matrix, and discloses a kind of inorganic fiber matrix for sand control and preparation method, comprising the following steps: S1, preparation inorganic nutrient solution, water, ammonium nitrate, potassium dihydrogen phosphate, potassium sulfate and magnesium sulfate are mixed uniformly, obtain nutrient solution;S2, preparation medicament, antagonistic bacteria, bacillus subtilis, trichoderma harzianum and gram actinomycetes group are mixed, after mixing, the nutrient solution obtained in S1 step is added, and continue to mix until uniform.The present application is formed in the form of large pore by inorganic fiber matrix material as a whole, root system can pass through large pore and come out from the bottom and four of inorganic fiber matrix, and then fully wrap inorganic fiber matrix, using the high water absorption of inorganic fiber matrix, can swell and absorb water when watering, achieve the characteristics of good root water absorption effect, when plant growth, can continuously absorb the moisture of inorganic fiber pore, achieve the effect of good water retention, and then improve the survival rate of plant.

The application belongs to the technical field of microorganisms, and particularly relates to an actinomycete strain with antagonistic activity against fungi and application thereof. The actinomycete strain with antagonistic activity against fungi is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 36167. The actinomycete fermentation liquor prepared in the application has high antagonistic activity against plant disease fungi, tinea pedis pathogenic bacteria and the like, and can solve the problems of drug resistance and environment in the prior art.

The invention discloses an organic fertilizer for regulating acid soil and a preparation method thereof, and belongs to the technical field of microorganisms. The organic fertilizer is prepared from rice husk biochar, an algae extract, mineral powder, decomposed and fermented pig manure, a microbial agent, urea and an adhesive. According to the invention, the algae extract, the kelp, the seaweed and the ascophyllum nodosum are cooperated to improve the pH value of the soil, increase the nutrient content, improve the soil structure and the like through multiple mechanisms, so that the acidic soil and the crop yield can be improved; the microbial agent composed of the introduced pseudomonas paraxanthomonas and actinomyces nanshan can improve the absorption and utilization of soil nutrients by plants and reduce the aluminum content and the heavy metal content in the soil at the same time. According to the organic fertilizer prepared by the invention, various substances have a synergistic effect, so that the pH value of acid soil is effectively increased, the soil fertility is improved, and the crop yield is increased.

The invention belongs to the technical field of microorganisms, and relates to an actinomycete strain and application thereof. The actinomycete strain is classified and named as amycolatopsis reevesii NVA54, the Latin name is Amycolatopsis reevesii, the preservation number of the actinomycete strain is CGMCC (China General Microbiological Culture Collection Center) 33989, and the preservation date is March 26, 2025. The invention further discloses a bacteriostatic agent containing the actinomycetes strain and application of the bacteriostatic agent and the actinomycetes strain. The actinomycete strain can obviously inhibit pathogenic bacteria of root rot, an obvious antibacterial zone is formed, and the antibacterial rate is not lower than 87.53%.

The present application relates to the technical field of PGPR compound microbial agent, in particular to a preparation equipment of acid-regulating, salt-reducing and heavy-crop-resisting PGPR compound microbial agent, which comprises a microbial agent preparation equipment group composed of a hierarchical synergistic propagation module, a biological culture medium preparation module, a dynamic fermentation module, a vacuum freeze spray drying module and a control and conveying module. The preparation equipment and process of the acid-regulating, salt-reducing and heavy-crop-resisting PGPR compound microbial agent can separate three independent microbial chambers by sterile isolation plates, can realize the synchronous and efficient propagation of multiple strains according to the differentiated needs of nitrogen-fixing bacteria, phosphorus and potassium solubilizing bacteria and heavy-crop-resisting actinomycetes, and can improve the strain concentration and the activity after fusion. The three-stage temperature regulation, dissolved oxygen and stirring rate are used in combination with low-frequency ultrasonic activation of heavy-crop-resisting active ingredients to synergistically enhance the acid-regulating, salt-reducing and heavy-crop-resisting effect. The medium formula and the proportion of functional carriers are dynamically adjusted according to the salinity and pH value of the target soil to adapt to the saline-alkali heavy-crop-improving needs of different regions and different crops.

The application belongs to the technical field of microorganisms, and particularly relates to a kind of actinomycete, compound and its preparation method and application. The actinomycete is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.20306. The actinomycete can be separated and purified to obtain kailing ketone mycin. The compound has obvious stomach poison activity on greenhouse whitefly adults. When the compound concentration is 100 μg / mL and treated for 72 h, the corrected mortality rate reaches 90.30%, and the compound also has touch kill activity on greenhouse whitefly. When the concentration is 100 μg / mL and treated for 72 h, the corrected mortality rate reaches 82.55%.