251 results about "Actinomyces" patented technology

The invention relates to the technical field of soil function improvement, in particular to a rice field soil recarburization and capacity expansion method based on a charcoal-based organic fertilizer. The method comprises the following steps: crushing a biomass raw material, pyrolyzing and cooling to obtain biochar; immersing the biochar in a functional bacteria solution containing actinomycetes and bacillus for activating treatment to obtain modified biochar; mixing the modified biochar and decomposed straw, composting and fermenting to obtain organic fertilizers with different particle sizes; the large-particle-size organic fertilizer is applied to deep soil, the soil is covered with soil after straw is laid on the soil, and the small-particle-size organic fertilizer is applied to surface soil. Through layered application, the large-particle-size biochar repairs the deep soil structure and expands the carbon storage capacity, and meanwhile, the carbon stability is improved through carbon-nitrogen synergistic metabolism driven by functional bacteria, so that the carbon immobilization efficiency is improved, meanwhile, soil recarburization and expansion are achieved, the contradiction between carbon sequestration and structure degradation and nutrient imbalance in a traditional recarburization and expansion technology is solved, and the soil recarburization and expansion efficiency is improved. Synergic improvement of a carbon sink function and agricultural productivity is promoted.

The invention discloses an organic fertilizer for promoting plant growth and improving soil and a preparation method thereof, and belongs to the technical field of organic fertilizers. The organic fertilizer is prepared from the following raw materials: composted chicken manure, soybean meal, straw powder, sweet potato vines, humic acid, a compound microbial agent, rosmarinic acid and a stimulant. The compound microbial agent is prepared from madula angustifolia actinomycetes, pseudomonas stutzeri and bacillus velezensis, and the stimulant is a glucosamine sulfate solution. The rosmarinic acid, the glucosamine sulfate solution and the compound microbial agent are added into the organic fertilizer, the three components are compounded with the straw, the chicken manure and the like to form the organic fertilizer, and various substances have a synergistic effect, so that on one hand, the growth of Chinese cabbages is promoted, and the yield is increased; on the other hand, soil physicochemical properties are obviously improved.

The invention provides actinomycetes and application thereof in prevention and control of fungal diseases of edible fungi. The actinomycetes are streptomyces cellulosae, the strain number of the actinomycetes is P8-2, and the registration number of the actinomycetes in the China General Microbiological Culture Collection Center is CGMCC No.32350, hereinafter referred to as streptomyces cellulosae CGMCC No.32350. Experimental results show that the streptomyces cellulosae CGMCC No.32350 disclosed by the invention has an inhibition effect on the fungal diseases of the edible mushrooms, including Trichoderma koningii, T.harzianum and Fusarium equiseti, and the streptomyces cellulosae CGMCC No.32350 has no influence on the growth of the edible mushrooms.

The invention relates to a mine solid waste-based composite saline-alkali soil modifier as well as a preparation method and application thereof, and belongs to the technical field of soil remediation and solid waste recycling. The modifier is prepared from volcanic rock solid waste, rice husk and mussel shell as raw materials through oxalic acid activation, microbial fermentation carbonization and humic acid chelation, and comprises 50-70 parts of activated stone powder-containing porous particles, 20-30 parts of rice husk-based charcoal, 10-20 parts of humic acid chelated magnesium-calcium powder and 3-5 parts of a composite binder. The preparation method comprises the following steps: ultrasonically activating the stone powder oxalic acid, and puffing and granulating with the binder; inoculating the rice hulls with salt-tolerant bacillus and actinomycetes for aerobic fermentation and carbonization; and the shell is activated by oxalic acid and then is loaded with a humic acid calcium magnesium compound. The modifier is applied according to 50-200kg / mu, so that the nutritional ingredients and the air permeability can be improved. According to the method, solid waste recycling, solid waste consumption and landfill cost and CO2 emission reduction are achieved, and the method is suitable for ecological restoration of the coastal saline-alkali soil and has both environmental protection and economic benefits.

The invention provides p-hydroxymandelic acid synthetase, a gene, a mutant of the p-hydroxymandelic acid synthetase and application of the p-hydroxymandelic acid synthetase in asymmetric synthesis of (S)-mandelic acid. The p-hydroxymandelic acid synthetase disclosed by the invention is derived from actinomycetes Actinokineospora auranticola, the amino acid sequence of the p-hydroxymandelic acid synthetase is as shown in SEQ ID NO.1, and a mutant of the p-hydroxymandelic acid synthetase is obtained by performing single-point mutation or multi-point combined mutation on glutamic acid at the 187th site, isoleucine at the 189th site and threonine at the 211th site of the amino acid sequence as shown in SEQ ID NO.1. The p-hydroxymandelic acid synthetase and the mutant thereof can convert phenylpyruvic acid into (S)-mandelic acid with high optical purity, have the advantages of mild reaction conditions, simplicity and convenience in operation, safety, environment friendliness and the like, and have a very good industrial application prospect.

The invention discloses a high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation. According to the high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation, yield increase and quality improvement are achieved through fungus material composite strengthening treatment and a composite strain synergistic inoculation technology. The method comprises the following steps: (1) fermenting a tilia brachypoda substrate by lactobacillus plantarum and soaking in a nano-composite nutrient solution to form a high-activity bacterial material; (2) nitrogen-fixing bacteria, disease-resistant actinomycetes and armillaria mellea are mixed and inoculated according to the proportion of 3: 1: 1, nitrogen supply is improved, and the occurrence rate of black rot is reduced; 3) activating a GASA stress-resistant gene of the seed hemp through a pulsed electric field (5 kV / cm, 10 microseconds), and enabling the germination rate to reach 88.5% in combination with a calcium signal channel strengthening technology; according to the method, the yield per mu of gastrodia elata is increased by 30%-40%, the gastrodin content is increased by 30% or above, the chemical pesticide dosage is reduced by 55%, the strain passage purity is stable, the method is suitable for large-scale planting in multiple climate areas, and an innovative solution is provided for green production of traditional Chinese medicinal materials.

The invention discloses a microbial decomposing inoculant for straws and a preparation method thereof, and belongs to the technical field of microorganisms. The microbial decomposing inoculant for straw is prepared from the following raw materials in parts by weight: 15-18 parts of functional microbial liquid, 3-5 parts of neutral protease, 1-3 parts of laccase, 20-25 parts of a nano-porous diatomite carrier, 5-7 parts of a humectant, 2-3 parts of humic acid chelated trace elements and 2-4 parts of an inducer. The preparation method comprises the following steps: screening talaromyces thermophilus, Guangxi thermoactinomycetes and arthrobacter habitualis to form a functional microbial liquid, adding a laccase-protease compounding system to cooperate with the physical adsorption effect of diatomite, and adding humic acid chelated trace elements to form an ion exchange network with polyglutamic acid; straw components are efficiently degraded through a triple mechanism of'enzymatic degradation-element supplementation-microenvironment regulation and control ', the decomposition process is accelerated, and the straw compost quality is improved.

The present invention belongs to the field of microbial technology and specifically relates to a salt-tolerant growth-promoting composite bacterial agent and its application. The active ingredients of the salt-tolerant growth-promoting composite bacterial agent are actinomycete S36, deposited with CGMCC No. 32928, Trichoderma M2, deposited with CGMCC No. 41651, and Bacillus velezensis Y10, deposited with CGMCC No. 32927. The present invention screened and obtained a composite bacterial agent obtained by mixing these three bacteria, which can improve the resistance of cotton and wheat to salt stress, providing a new microbial resource for improving the salt tolerance of cotton and wheat.

The invention discloses a method for preparing a biological bacterial fertilizer from coal and microorganisms, and belongs to the technical field of biological fertilizers. The method comprises the following steps: crushing coal to 80 meshes to obtain coal powder; adding a compound fertilizer accounting for 3% of the total fertilizer mass; a composite additive accounting for 1% of the total mass of the fertilizer is added, and the additive comprises 95% of mushroom sticks, 3% of glucose and 2% of microbial flora (bacillus subtilis, actinomycetes, lactic acid bacteria and the like are mixed according to the proportion of 1: 1: 1: 1); the raw materials are mixed and granulated. The organic matter content of the prepared biological bacterial fertilizer is larger than or equal to 30%, the effective viable count is larger than or equal to 2 * 10 < 7 > CFU / g, the heavy metal content is remarkably lower than the national standard, and cadmium is smaller than or equal to 0.5 mg / kg, lead is smaller than or equal to 30 mg / kg, and chromium is smaller than or equal to 80 mg / The method realizes efficient biotransformation of coal resources, and is suitable for soil improvement and organic agricultural production.

The invention discloses a bifidobacterium animalis subsp. Lactis BA-C53 and an application of the bifidobacterium animalis subsp. Lactis BA-C53 in products for improving bone health. The bifidobacterium animalis subsp. Lactis BA-C53 is classified and named as bifidobacterium animalis subsp. Lactis Bifidobacterium animalis subsp. Lactis BA-C53, the preservation number is CCTCC NO: M 2024460, the preservation unit is China Center for Type Culture Collection, and the preservation time is March 11, 2024. The bifidobacterium animalis subsp. Lactis BA-C53 can effectively improve the steady state of intestinal flora of a mouse with osteoporosis induced by estrogen deficiency, so that the relative abundance of phylum firmicalis and phylum actinomycetes is increased, the relative abundance of phylum bacteroides is reduced, and then short-chain fatty acid is generated to activate intestinal immunity and maintain the integrity of an intestinal barrier, so that the osteoporosis caused by estrogen deficiency is inhibited. Meanwhile, osteoclast differentiation can be inhibited by regulating NFATC1, RANKL and OPG pathways, then osteoporosis is relieved, and the compound can be widely applied to preparation of food and drugs.

The invention provides compost and a preparation method thereof, and relates to the technical field of organic fertilizers, the compost comprises the following components by weight: 900-1100 parts of crop straw, 200-300 parts of animal excreta, 100-200 parts of an organic auxiliary material, and 1.5-7.5 parts of a complex microbial inoculant; the complex microbial inoculants comprise lactic acid bacteria, bacillus licheniformis, thermophilic trichoderma, actinomycetes and white rot fungi. The preparation method of the compost mainly comprises the following steps: stacking the crop straws and the organic auxiliary materials layer by layer, and compacting; uniformly sprinkling animal excrement on each layer, and then inoculating a complex microbial inoculant; punching a hole in the top of the pile to monitor the moisture content in the pile in the composting process; pile turning treatment is carried out in the composting process. According to the invention, the complex microbial inoculants are adopted for compost maturing treatment, so that the maturing speed can be increased, the maturing effect can be improved, and the content of nutrient substances in compost is increased.

The invention discloses a preparation method of an actinomycetes and waste mushroom matrix composite, belongs to the technical field of environmental microorganisms, solves the problems of low resource utilization rate, serious environmental pollution and the like in the existing waste mushroom matrix treatment, and overcomes the defects of loose soil structure and poor erosion resistance caused by direct returning of the waste mushroom matrix to the field. The method comprises the following steps: separating actinomycetes strains from soil, purifying and culturing the actinomycetes strains into a bacterial solution, crushing a waste mushroom substrate, adjusting the water content and the initial pH value, inoculating the bacterial solution into a pretreated substrate, and culturing in a dark place to form a composite material with biological activity and structural stability. Lignocellulose in a matrix is efficiently degraded by utilizing cellulase secreted by actinomycetes and lignin peroxidase, and a three-dimensional cross-linked structure is formed through a hypha network and humic acid-minerals, so that the mineralization efficiency of organic matters and soil agglomeration are improved. The composite material can be applied to the field of water and soil loss prevention and control, and efficient conversion of waste resources and collaborative improvement of soil ecological functions are achieved.

The invention relates to a formulation of microbial consortia selected from (a) Bacilli group, (b) Pseudomonas group and (c) Actinomycete group having nitrogen fixing capacity, when the bacteria comes in contact with soil or any aqueous media it gets active, which further helps for plant growth and makes the soil environment more suitable for plant.

The invention relates to the field of microorganisms, and discloses application of lactococcus lactis in a product for improving sleep. The lactococcus lactis is named as WHH2078, is separated from yoghurt butter collected from a country in Sankarst city in Tibet Autonomous Region in China, is preserved in China General Microbiological Culture Collection Center on February 25, 2021, has a preservation number of CGMCCNO.21831, and is classified and named as Lactococcus lactis. The Lactococcus lactis is named as Lactococcus lactis, and the Lactococcus lactis is named as Lactococcus lactis. The lactococcus lactis has the effects of shortening the sleep latency, prolonging the total sleep time, improving 5-hydroxytryptamine and gamma-aminobutyric acid of the brain, serum and colon, lowering the corticosterone level, regulating back the diversity of intestinal microorganisms alpha, regulating up the abundance of intestinal acidobacilli and lowering the abundance of actinomycetes, desulfurization bacilli and verruca microflora. The sleep disorder can be effectively improved.

The present invention discloses an organic fertilizer for promoting plant growth and soil improvement and a preparation method thereof, and belongs to the technical field of organic fertilizers. The organic fertilizer comprises the following raw materials: decomposed chicken manure, soybean meal, straw powder, sweet potato vine, humic acid, composite microbial agent, rosmarinic acid, and a stimulant. The composite microbial agent is composed of Actinomyces millefolius, Pseudomonas stutzeri, and Bacillus velezensis, and the stimulant is a glucosamine sulfate solution. Rosmarinic acid, glucosamine sulfate solution, and composite microbial agent are added to the organic fertilizer of the present invention. These three components are compounded with straw, chicken manure, etc. to form an organic fertilizer. The synergistic effect of multiple substances promotes the growth of cabbage and increases yield on the one hand; on the other hand, it significantly improves the physical and chemical properties of the soil.

The invention provides a pill-type organic fertilizer special for vegetables. The pill-type organic fertilizer is prepared from the following raw materials in parts by weight: modified biochar, fermented livestock and poultry manure, plant source protein zymolyte, a complex microbial inoculant, a trace element supplement and a binder, and a method therefor; the complex microbial inoculants comprise actinomycetes, bacillus amyloliquefaciens and trichoderma harzianum; the trace element supplement comprises EDTA (Ethylene Diamine Tetraacetic Acid) chelated zinc, EDTA chelated iron and boric acid. Therefore, on one hand, the organic fertilizer can effectively improve the facility agriculture soil environment, adjust the soil acidity and alkalinity, reduce the salinization degree, promote balance and stability of microbial communities and restore and improve the ecological function of soil; on the other hand, the utilization rate of the fertilizer can be greatly improved, and the plant-source proteolysis provides a quick-acting nitrogen source to promote root development; the microelement supplement meets the requirements of vegetables on microelements; nutrients are balanced, vegetable stress resistance is enhanced, and photosynthetic efficiency is improved; and the binder enables the fertilizer to form uniform particles, is suitable for mechanical fertilization, and improves the fertilization efficiency.

The invention discloses a microbial agent for increasing manure compost temperature and application thereof in high-temperature compost, and belongs to the field of agricultural solid waste treatment. The microbial agent for increasing the manure composting temperature comprises phanerochaete chrysosporium, bacillus stearothermophilus, penicillium oxalicum, streptomyces, common thermoactinomycetes and bacillus subtilis. The livestock padding manure high-temperature clean composting technology is established through adjustment and optimization, the purposes that the temperature of the padding manure is increased fast, the high-temperature period is long, the composting period is shortened, base materials are fully degraded are achieved, reference can be provided for resource utilization of livestock and poultry breeding solid waste, and important application value is achieved.

The invention discloses a polysaccharide polymer, application of the polysaccharide polymer and a preparation method of the polysaccharide polymer. The polysaccharide polymer QPS capable of remarkably selectively inhibiting harmful bacteria in aquaculture is separated and purified from the water mold antagonistic actinomycete strain QHV2 for the first time, can effectively inhibit mycelial growth and spore germination of water mold / branchiomycosis and destroy mature biological membranes, is stable in activity, free of toxic effects on fry and good in biological safety, and can be applied to the field of aquaculture. The material can be used as a candidate material for research and development of a fish pathogenic fungus biological prevention and control agent. Meanwhile, the polysaccharide polymer QPS avoids pollution of harmful substances to a water body when a QHV2 fermentation product is directly applied, can selectively inhibit staphylococcus aureus, saprolegnia, branchiomycosis and other harmful bacteria in aquaculture, and has no inhibition or weak inhibition on pseudomonas pseudoalcaligenes, bacillus and other beneficial bacteria in aquaculture. And the application effect is better.

The invention belongs to the field of microbial agents, and particularly relates to a compost heat production method of a ganoderma lucidum basswood planting waste substrate, which comprises the following steps: mixing the ganoderma lucidum basswood planting waste substrate with chicken manure to obtain a fermentation raw material; paving the fermentation raw materials in a fermentation container layer by layer; a complex microbial inoculant is uniformly sprayed on the surface of each layer of fermentation raw material; the complex microbial inoculant is prepared from the following strains in percentage by weight: 10 to 25 percent of bacillus cereus, 5 to 10 percent of bacillus smith, 5 to 10 percent of geobacillus thermodenitrificans, 20 to 40 percent of bacillus licheniformis, 5 to 24 percent of ureibacillus thermophilus and the balance of actinomyces thermophilus; the oxygen content is controlled to be 5-15%; the 50-60 DEG C high-temperature period is maintained for 180-210 days, the heat exchange pipe located between the two layers of fermentation raw materials is connected to a heating system in the high-temperature period, and the system is suitable for agricultural circular economy and distributed energy supply scenes.

The invention discloses a high-activity actinomycete for killing root-knot nematode as well as a culture method and application of the actinomycete. The name of the actinomycetes is Streptomyces griseorubens WZSF-1, the classification name of the actinomycetes is Streptomyces griseorubens, and the preservation number of the actinomycetes in the Guangdong Microbial Culture Collection Center is CDMCC (China General Microbiological Culture Collection Center) 65368. The strain disclosed by the invention has very good insecticidal activity on meloidogyne incognita and elephant bean meloidogyne, and the insecticidal activity can reach 90.22% and 94.76% respectively.

The invention relates to the technical field of microbial fertilizers. The invention aims to provide a fruit and vegetable biological source bacterial fertilizer system which comprises the following raw materials in parts by weight: 600 parts of wormcast, 200 parts of straw powder, 100 parts of humic acid, 1 part of a microbial agent and 5 parts of brown sugar. The microbial agent is prepared from bacillus subtilis, bacillus megatherium and actinomycetes according to the weight ratio of 5: 3: 2. According to the invention, the natural wormcast is used as a microbial carrier, and by virtue of the unique physical structure and microbial basis of the wormcast and in combination with a functional strain compounding technology, the defects of the traditional fertilizer can be effectively overcome.

To provide a system including novel nanopores having different structures and recognition sites that improve sequencing accuracy and / or provide different error profiles.SOLUTION: Provided are systems relating to the field of nanopores and their use in various applications, e.g., analysis of biopolymers and macromolecules, typically by making electrical measurements during translocation through the nanopore, comprising a funnel-shaped proteinaceous nanopore comprising alpha-helical pore-forming toxins that are members from the actinoporin family of proteins, more particularly Fraga Ceatoxin C (FraC), a variant FraC, a FraC paralog or a FraC homolog.SELECTED DRAWING: None

The present application relates to the technical field of eutrophication water treatment, and discloses a preparation method and application of active substances of algicidal actinomycetes, which comprises the following steps: (1) inoculating actinomycetes into a shaking flask containing a culture medium, wherein the culture medium comprises carbohydrates, salts, glutamic acid and cyanobacteria, and then placing the shaking flask in a culture box for oscillation culture to obtain a first-stage seed liquid; (2) inoculating the first-stage seed liquid into a first-stage seed tank according to a volume fraction of 10-20% to obtain a second-stage seed liquid; (3) inoculating the second-stage seed liquid into a fermentation tank according to a volume fraction of 10-20% to obtain an actinomycete fermentation liquid; and (4) centrifuging the actinomycete fermentation liquid to obtain a supernatant and mixing the supernatant with a stabilizer. Glutamic acid can promote the actinomycetes to secrete more active substances during the fermentation process, and cyanobacteria can enable the actinomycetes to maintain the ability to remove algae during the fermentation process to secrete corresponding active substances. The stabilizer can inhibit the growth of miscellaneous bacteria to maintain the stability of algae removal.

The present invention discloses an actinomycete with antagonistic effect against the pathogen of cucumber damping-off and its application. The actinomycete is Streptomyces aureusensis Q1C-44, which has antagonistic effect against Rhizoctonia solani of cucumber, can effectively inhibit the hyphal elongation of the pathogenic fungus, and can be used to control damping-off caused by Rhizoctonia solani of cucumber.

The present invention provides a prophylactic or therapeutic composition for graft-versus-host disease (GVHD). There is provided a prophylactic or therapeutic composition for GVHD, which comprises bacteria belonging to any genus selected from the group consisting of the following genera: Blautia, Clostridium, unclassified Clostridiales, Actinomyces, Parabacteroides, Lachnoclostridium, Bacteroides, Faecalibacterium, unclassified Lachnospiraceae, Roseburia, Ruminococcus, unclassified Firmicutes, Dorea, Phascolarctobacterium, Sutterella, Megamonas, Collinsella, Eubacterium, and Coprococcus, etc., or any combination of bacteria belonging to these genera.

The invention discloses application of an NADH kinase element sPos5 in improving the yield of secondary metabolites of actinomycetes, and belongs to the technical field of genetic engineering. In order to improve the yield of secondary metabolites of actinomycetes, an NADH kinase element sPos5 with a nucleotide sequence as shown in SEQ ID NO.1 is obtained by screening NADH kinase capable of improving intracellular NADPH reducing power of a strain, a recombinant vector and recombinant bacteria are constructed by utilizing the NADH kinase element, and the recombinant bacteria can be used for preparing the secondary metabolites of the actinomycetes. Fermentation experiments show that the NADH kinase element sPos5 can meet the requirement for reducing power in the secondary metabolite synthesis process, and the yield of the secondary metabolite of actinomycetes is increased.

The invention relates to an actinomycete preparation and application thereof. The actinomycete preparation is a product obtained by culturing actinomycetes with insect fecula. When the actinomycetes preparation is stored for five months, half a year or one year or more, the content of the actinomycetes in the actinomycetes preparation is 1 * 10 < 11 > CFU / g or more.

The invention discloses a naphthocycline type pentacyclic aromatic polyketone compound. The structural formula of the naphthocycline type pentacyclic aromatic polyketone compound is as shown in a formula (I): # imgabs0 #. According to the invention, six naphthocycline type pentacyclic aromatic polyketone compounds are separated from a rice fermentation product of actinomycetes Streptomyces sp.NAK5495 for the first time, and the six naphthocycline type pentacyclic aromatic polyketone compounds are separated from rice fermentation products of actinomycetes Streptomyces sp.NAK5495; according to the present invention, with the medium-pressure liquid chromatography (MPLC) system and the semi-preparative HPLC separation and purification technology, the efficient separation and purification of the naphthocycline pentacyclic aromatic polyketones are achieved, and the recovery rate and the purity of the target compound are improved.

The invention belongs to the technical field of soil conditioners and fertilizers, and particularly relates to a bio-organic fertilizer for improving salt and alkali as well as a preparation method and application of the bio-organic fertilizer. The method comprises the following preparation steps: (1) carrying out pyrolysis treatment on dry agricultural and forestry wastes or livestock and poultry manure under anaerobic and 400-700 DEG C conditions, and crushing to obtain a biochar material; and (2) mixing and grinding the obtained biochar material, soybean meal and water, and then inoculating a bacillus subtilis activation solution and an actinomycetes activation solution for fermentation treatment to obtain the saline-alkaline improved bio-organic fertilizer. According to the bio-organic fertilizer, the biochar prepared from agricultural and forestry waste or animal dung is used as a main raw material, EPS generated through in-situ loading microbial fermentation metabolism can synergistically improve the improvement effect of the obtained organic fertilizer on saline-alkali soil and the passivation effect on heavy metal, absorption of crops on the heavy metal is remarkably reduced, and the fertilizer efficiency is improved.

The invention belongs to the technical field of soil improvement, particularly relates to a compound microorganism saline-alkali soil conditioner as well as a preparation method and application thereof, and aims at solving the problems that the existing soil conditioner mostly adopts a single strain or a simple combination, is difficult to colonize in a harsh saline-alkali environment, has a single function, cannot systematically improve the physical and chemical properties of soil, is slow in organic material decomposition and is difficult to promote. In order to solve the problems that in the prior art, the soil conditioner is not durable in effect due to the fact that the soil conditioner is prone to failure due to saline-alkali stress, the following scheme is provided: the soil conditioner comprises the following raw materials in parts by weight: 5-10 parts of bacillus; by using bacillus, streptomyces, saccharomycetes, actinomycetes, nitrogen-fixing bacteria, phosphate bacteria, potassium bacteria, pyroligneous liquor, seaweed extract, amino acid chelate, potassium fulvate, citric acid and monopotassium phosphate, the content of organic matters in soil can be effectively increased, the aggregate structure of the soil is improved, a more lasting soil improvement effect is achieved, and the soil conditioner has a good application prospect. And the use frequency and cost of the modifier are reduced.