906 results about "Actinomyces" patented technology

The present invention relates to growing and testing microorganisms in a multitest format which utilizes a gel forming matrix for the rapid screening of clinical and environmental cultures. The present invention is suited for the characterization of commonly encountered microorganisms (e.g., E. coli, S. aureus, etc.), as well as commercially and industrially important organisms from various and diverse environments (e.g., the present invention is particularly suited for the growth and characterization of the actinomycetes and fungi). The present invention is also particularly suited for comparative analysis of phenotypic differences between cell types, including strains of microorganisms that have been designated as the same genus and species, as well as other cell types (e.g., mammalian, insect, and plant cells).

A biological deodouring process for the stink gas features that the stink gas is forced to pass through the biologic filter material prepared from anticorrosion plant fibres, porous materia, or theirmixture through inoculating microns (yeast, bacteria, etc) to it. Its apparatus is composed of blower, gas washing pool and biological filter tower. Its advantages are simple apparatus, high effect, low cost, and no secondary pollution.

The invention relates to a microbe soil repairing additive and process for preparation. The process for preparation comprises that liquid microbe fertilizer containing photosynthetic bacteria, bacillus, actinomycete and lactic acid bacteria are absorbed to one or a plurality of natural organic fertilizers as grass peat and zeolite powder or the like, to be mixed proportionally. The invention further relates to the application of the microbe soil repairing additive for repairing soil. The invention can lead useful microbe into soil, uses microbe technique to transfer, absorb, degrade, and convert the contaminant in soil, adjust soil pH value, eliminate soil blocking, and restrain soil-borne disease, thereby improving soil structure, to return soil to original ecologic structure. And the viable bacteria group of the invention is planted in plant root, to quickly grow and deeply insert plant root, thereby improving yield, quality and level of agricultural product, as one ideal fertilizer of organic agriculture, with wide application in agriculture continuous development.

The invention relates to an alga microorganism fertilizer agent and a preparation method thereof and belongs to the technical field of aquiculture and the like. The alga microorganism fertilizer agent is fermented in such a way that monosodium glutamate waste liquid, alga paste, photosynthetic bacteria, actinomycetes, azotobacters and bacillus subtilis are compounded, and meanwhile, chitosan, chitosan oligosaccharide, microelements and molasses are added according to appropriate parts. The alga microorganism fertilizer agent is formed in such a way that the monosodium glutamate waste liquid is adopted for fermentation, and the raw materials preparation is simple; high-content compound bacteria are contained (the content of the bacillus subtilis is larger than three hundred million/mL, and the total content of the photosynthetic bacteria, the actinomycetes and the azotobacters is larger than one hundred million/mL) and the fertilizing effect is obvious; and the alga microorganism fertilizer agent also has a function of purifying water body, has a wide application range, and is suitable for sea-water and fresh-water cultivation.

The invention relates to a microbial agent used for producing a soil modifying agent by fermentation, consisting of fiber monad, bacillus, lactobacillus, methanobacteria, thiobacillus, streptomycete, high-temperature actinomyces, high-temperature monad, azotobacteria, nitration monad, nitration bacilus, rhizobium, koji mold, leaven, blue mold, detritus mold, rhizopus as well as mycorrhizal fungi and substrate. In addition, the invention also relates to a soil modifying agent produced by the fermentation of the microbial agent, application thereof and the like.

The present invention relates to growing and testing microorganisms in a multitest format which utilizes a gel forming matrix for the rapid screening of clinical and environmental cultures. The present invention is suited for the characterization of commonly encountered microorganisms (e.g., E. coli, S. aureus, etc.), as well as commercially and industrially important organisms from various and diverse environments (e.g., the present invention is particularly suited for the growth and characterization of the actinomycetes and fungi). The present invention is also particularly suited for comparative analysis of phenotypic differences between cell types, including strains of microorganisms that have been designated as the same genus and species, as well as other cell types (e.g., mammalian, insect, and plant cells).

The present invention is one kind of soil improving agent for vegetable plot and its preparation process and application, and belongs to the field of soil improving microbe preparation technology. The soil improving agent consists of turf, diatomite, microbe preparation, peanut cake and fly ash, and its microbe preparation includes Bacillus subtilis fermenting liquid, actimomycetale fermenting liquid and trichoderma fermenting liquid in the weight ratio of 1 to 0.5-3.5 to 0.5-6. It is applied to soil after being mixed in the field, can improve soil, and raise crop yield and quality obviously.

The invention relates to an organic materials zymolysis complex bacillus agent in the field of zymolysis bacillus agent technology. It comprises: low temperature bacteria, Bacillus cereus, gliotoxin, pseudomonas, slim streptomycete, high temperature bacteria and high temperature actinomyces with the weight ration 4:2:1:1:3:2:2. The low temperature bacterium is enzyme yeast with the heat resisting temperature below 20 deg. The high temperature bacterium is enzyme yeast with the heat resisting temperature below 70 deg. The heat resisting temperature below 70 deg of the high temperature actinomyces bellows 70 deg.

The invention discloses a microorganism compound microbial inoculum, the strains of which comprise bacillus, soil actinomycetes, microzyme, mildew and photosynthetic bacteria. By using the microbial inoculum for straw fermentation, a result indicates that the microbial inoculum can make straws quickly decay and transfer to organic fertilizer required for the growth of plants, thereby the content of soil humus is increased, and the content of microorganism and trace elements in soil is improved. The physicochemical property of soil is improved, and the capability of water and fertility preserving of soil is increased. The soil permeability can also be improved so as to inhibit the happening of crop pests, and the output and quality of products can be improved. The microbial inoculum has the advantages of convenient use, low cost and obvious effect.

The invention relates to the greenhouse crop planting field, in particular to compound microbes for reducing and disinfecting greenhouse soil and a soil disinfection method, and solves the problem of multiple defects in the prior art. The compound microbes comprise bacteria, actinomycetes, saccharomycetes and moulds. The soil disinfection method includes the steps of selecting June to September as a disinfection period to apply crop straws, organic fertilizer and the compound microbes on the soil after the crops are harvested; deeply plowing soil layers to form small ridges; covering ridge surfaces with plastic film, filling enough water under the film till the soil humidity achieves 100%, and sealing the mulching film and covering the greenhouse plastic film for 20 to 25 days; and uncovering the greenhouse plastic film and removing the mulching film for planting. According to the compound microbes for reducing and disinfecting greenhouse soil and the soil disinfection method, the method is non-toxic, harmless, pollution-free and residue-free, pathogenic bacteria, ova and the like in soil plough layers are killed, soil organics are increased, the soil physical and chemical structure is improved, soil fertility is improved, and good foundation is laid for improving product qualities.

The invention discloses a preparation method of compound microorganisms and a recombination enzyme solid agent which can degrade the ammonia- nitrogen and nitrite in the aquicultural water environment. The invention uses universal liquid medium to culture bacillus subtilis, nitrifying bacteria, photosynthetic bacteria, denitrifying bacteria, yeast, actinomyces etc six under different temperatures. Then the culture liquid is centrifugally separated and then the bacteria body is collected. The bran is added into the bacteria body as an adsorbent. The mixture of the bacteria body and the bran is boiled and dried on a fluidized bed. And the freeze-dried powder of compound microorganisms is obtained. Then proteases, lipase, and amylase are added to make the compound microorganisms and the recombination enzyme solid agent. The invention is put into the aquicultural water that needs to be dealt with and becomes nitro-nitrogen by the decomposition of proteases, lipase, and amylase and the ammonification of bacillus subtilis. Next, the nitro-nitrogen becomes nitrogen which is released into the air. So the invention can take effect quickly on completely degrading the ammonia- nitrogen and nitrite in the aquicultural water.

The invention discloses a microorganism concrete brick and a preparation method thereof. The microorganism concrete brick is formed by mixing cement, gravel, sawdust (or straw scraps, scraps of paper, and the like), mixing mixed microorganism with water according to a certain proportion, forming by a mold and then naturally hardening, wherein the mixed microorganism comprises six floras including bacillus, nitrobacteria, photosynthetic bacteria, saccharomycetes, lactobacillus and actinomycetes. The microorganism concrete brick has excellent water quality purifying function, can continuously play roles for a long time, and is widely applicable to purify various water qualities such as rivers, lakes, canal water, swimming pools, fishponds and the like.

The invention discloses a mixed seeding green manure dressing method for a tobacco field. According to the method in the invention, mixed seeding is carried out on ryegrass and Chinese trumpet creeper in an equal ratio from the first ten days of September to the first ten days of October, wherein the seeding amounts of the ryegrass and Chinese trumpet creeper are 1.5-2.0kg/mu respectively, basic seedling amount is 70000-100000 plants/mu, 1500-2000kg of fresh samples are turned over and buried in per mu of soil with the depth of 10-20cm at the time of 40-45 days before flue-cured tobacco transplanting, and the pure nitrogen applying amount is reduced by 1.8-2.4kg/mu when tobacco seedlings are transplanted. The method comprises soaking seeds, seeding, fertilizer water management, turning over and burying and fertilizer application when the tobacco seedlings are transplanted. By using the method, the problems that when the fertilizer is singly applied for a long term, the carbon-nitrogen ratio in the tobacco field is reduced, nutrient is not balanced, effective fertilizer supplying capacity is weak, and the chemical components of tobacco leaves are inconsonant are solved. The method has the advantages that quick-action nutrient content, total bacterial count and nitrobacteria and actinomycetes quantities in current-season tobacco transplanting soil are obviously increased, the nutrient of the tobacco plants is consonant, the potassium-increasing base-reducing effect of the tobacco leaf is obvious, aroma quality is improved, and the benefit of tobacco planting is greatly improved. The method has good popularization value in the aspect of modifying the long-term continuous cropping tobacco field.

The invention discloses a composite bacterial preparation as well as a preparation method and application thereof, which belong to the technical field of agricultural biochemistry. The composite bacterial preparation comprises the following components in parts by volume: 10-40 parts of brevibacillus brives fermentation bacterial liquid, 10-40 parts of pseudomonas fluorescens fermentation bacterial liquid and 15-45 parts of sugarcane thermophilic actinomyces, wherein the bacterial count in each mol of fermentation liquid is 0.4-0.7 billion. The strains of brevibacillus brives, pseudomonas fluorescens and sugarcane thermophilic actinomyce are separated from rabbit dung and fungi residues; and fermentation bacterial liquids of the separated strains are mixed according to a proper proportion to form the composite bacterial preparation. The composite bacterial preparation disclosed by the invention can be used for accelerating the degradation of organic materials and reducing the conversion of organic nitrogen into gaseous nitrogen, so that the composite bacterial preparation can present stronger ammonia-suppressing and decomposing capabilities in the fermentation process of pig manurecompost; and a smaller amount of ammonia gas is released while thorough decomposition of pig manure is accelerated.

The invention discloses a preparation method of microbial pesticide for controlling vegetable fungal diseases, belonging to the microbial technical field, which utilizes a streptomyces diastatochromogenes D isolated and screened from a soil sample to prepare various formulation pesticides with the steps of the activation cultivation of strain, fermentation cultivation, leavening extraction and distribution of pesticide auxiliary material to the purified active composite, wherein the soil sample is acquired from Lin' an Tianmu mountain in Hangzhou, Zhejiang. The preparation method of microbial pesticide for controlling vegetable fungal diseases is safe and can be used for controlling cucumber fusarium wilt, tomato gray mold, and other vegetable fungal diseases; and the controlling effect is a little better than normal chemical agents.

The purpose of this invention is to dispose of and effectively utilize waste of the skin of a bamboo shoot, young bamboo and parent bamboo by using one or both of the skin of the bamboo which is waste in a boiling work plant of the bamboo shoot and the young bamboo which is a grown bamboo shoot as main components, adding the parent bamboo therein and composting the raw materials for a short time at a low cost. The subject fermented fertilizer is produced by adding useful microorganisms containing one or more kinds of actinomyces, photosynthetic bacteria, fungi, lactobacilli, yeast and aspergillus bacteria into the composting raw material mainly using one or both of the skin of the bamboo shoot and the young bamboo, and if need, the parent bamboo, further adding at least one of a fermentation accelerating assistant, a water control agent and a nutritious agent of the microorganisms if needed and mixing and stirring them. The fermented fertilizer containing a growth promotor contained in the bamboo shoot or the young bamboo is produced by composting the skin of the bamboo shoot or the young bamboo used as the main components and becomes the fertilizer remarkably effective for the growth promotion of plants.

The invention relates to the technical field of microbes, and discloses a separation method for plant endophyte. The separation method comprises the following steps: (1) pretreating plant tissue; (2) culturing plant tissue; and (3) separating endophyte. The disclosed separation method for plant endophyte is capable of effectively removing surface bacteria, fungi, actinomyces and other microbes, and avoiding endophyte separation effect caused by mixing of mixed bacteria. Endophyte in the plant tissue recovers growth after tissue culture, and disinfection processing does not need performing in operation of separating endophyte, so that the damage effect of a disinfectant on plant tissue and endophyte is substantially reduced, the survival probability of endophyte is increase, and the number of endophyte obtained through separation is obviously improved.

The invention belongs to the applied biology technical field, and relates to a water environmental microbiology reparation agent and a preparation method thereof. The reparation agent has the mass percent of the components in the microbiology total weight as follows: bacillus subtilis is 30-40 percent, bacillus licheniformis is 30-40 percent, ray fungi is 10-20 percent, denitrifying bacterium is 10-20 percent, and photosynthetic bacterium is 10-20 percent. The microbiology preparation of the invention has strong adaptability, can compensate the water body original bacterium when being put in water, quickly form the preponderant flora in the water body, produce the high-activity biological enzymes for quickening the decomposition of the left diet and the animal wastes, and produce the antibiotic substance to restrain the growing of harmful bacterium and reduce the occurrence of the illness as well as ensure the production of green marine products.

The invention relates to a novel microbial organic fertilizer with anti-actinomyces active compound bacteria and a preparation method thereof. The novel microbial organic fertilizer comprises a microbial inoculum and an organic fertilizer, wherein the microbial inoculum comprises colonies of Bacillus siliceous, Nocardia actinomyces, Bacillus subtilis, Bacillus thuringiensis, Brevibacillus laterosporus, azotobacter, Beauveria bassiana, Metarrhizium anisopliae, Trichoderma and Saccharomyces, and peptide proteins and rare-earth elements having unique effects on inhibiting wireworms and enhancing plant root system stress tolerancen; and the organic fertilizer comprises animal manure and plant fibers. The novel microbial organic fertilizer is prepared by fermenting the animal manure and plant fibers by using the microbial inoculum. The microbial organic fertilizer can quickly and effectively kill wireworm ova in soil, has strong effects on inhibiting and killing larvae and imagos, can continuously improve the growth environment of the plant root by using the growth of the microbial colonies and the stress tolerance of the peptide proteins, enables the plant to have strong roots, thick stems and green leaves, postpones the aging of the plant, and promotes prematurity and yield increase.

The invention relates to a seeding medium made of vinasse methane-sludge and a preparation method thereof, not only solving the problem of environment contamination caused by vinasse methane-sludge, but also effectively utilizing vinasse methane-sludge. The seeding medium comprises fresh sludge containing 90 percent of water after methane generated from vinasse in a methane tank, accessories, ferment strain I and ferment strain II, wherein the accessories comprise grass peat, vermiculite and perlite; the ferment strain I comprises saccharomycetes, lactobacillus, high-temperature actinomycetes and bacillus; and the ferment strain II comprises photosynthetic bacteria, lactobacillus, bacillus, saccharomycetes, actinomycetes and terrestrial cyanobacteria. The preparation method comprises the following steps of preparing fresh sludge, dehydrating, adding the ferment strain I for fermentation, adding the ferment strain II for secondary fermentation, air drying, adding accessories, crushing, screening and packing. The vinasse methane-sludge contains 40 to 50 percent of organic matter and 4.3 percent of available nitrogen (N), phosphorus (P) and kalium (K). The seeding medium made of vinasse methane-sludge has better hydrophilicity, and seeding can be carried out after loading the dry seeding medium and spraying water, thus saving both labor and time.

The invention provides an ecological restoring method of a river water body, comprising the following steps: (1) detecting pollution indexes of a river water body; (2) throwing composite microbial agents into a water body according to 5ppm to 9ppm; (3) transferring, translating and degrading matters in the river water body by the composite microbial agents; (4) throwing and/or planting water plants into a river; (5) detecting the pollution indexes of the river water body; and (6) repeating the steps from (2) to (5) from 15 days to 30 days until the river water body conforms to requirements. Each composite microbial agent comprises the components by weight percent: 5 to 10 photosynthetic bacteria, 10 to 15 bacillus subtilis, 5 to 10 nitrifying bacteria, 2 to 5 microzyme, 15 to 20 lactobacillus, 10 to 30 aspergillus, 10 to 30 acetobacter and 20 to 30 actinomyces. The ecological restoring method of a river water body aims at a river water body which carries out eutrophication treatment, leads the river water body to restore the bio-diversity of the river water body, radically restores ecology and has durable effect, low cost and simple use.

The invention discloses a leavening agent for poultry excrement, which comprises following microorganism fermentation liquor by volume ratio: 30-40% of bifidobacterium, 10-25% of lactic acid bacteria, 10-30% of bacillus megatherium, 10-25% of saccharomycetes, 10-15% of actinomycetes and 10-15% of acetic bacteria. The leavening agent can eliminate environmental fetor and restrain multiplication of malignant bacteria. Organic garbage in treated in composting mode, thereby recycling resources, reducing environment pollution, reducing sewer dirt and reducing odor. The poultry excrement is fermented into fertilizers and animal feed. The leavening agent for the poultry excrement is widely applied to fermentation of excrement and garbage.

The invention provides a production technology of organic manure for controlling nemathelminthiasis. The technology comprises the following operational steps: (1) aerobic actinomyces strain-liquid is prepared; (2) chinaberry materia medica or margosine extract leftover thereof which is 30 to 50 percent, sophora flavescens materia medica or matrine extract leftover thereof which is 20 to 40 percent, and pyrethrin materia medica or pyrethrin extract leftover thereof which is 20 to 40 percent are got to be respectively crushed into 40 to 60 screen meshes, then are mixed evenly and added into the strain-liquid in the step (1), and are piled to be fermented for a first time (for 7 to 15 days under the temperature ranging from 60 to 75 DEG C), and then are mixed to be fermented for a second time (for 7 to 15 days under the temperature ranging from 30 to 40 DEG C) to obtain the fermentation product; then amino acid raw powders, the weight of which is 10 percent of the fermentation product, are added; then the mixture is mixed evenly to obtain organic manure stroma; (3) 10 to 15 percent of the organic manure stroma in the step (2) and 85 to 90 percent of the organic manure are got and mixed evenly to obtain the organic manure for controlling the nemathelminthiasis. The technology is simple, the cost is low without pollution, the obtained product can prevent various nemathelminthiasis, the preventing effect thereof can reach more than 75 percent, the output is increased by 10 to 30 percent, and the production technology of organic manure for controlling the nemathelminthiasis is applied to field crops, garden stuffs, tobacco leaves, etc.

The invention discloses a compound bacterium agent and a method for accelerating fast maturity by inoculating compound bacterium agent to compost. The method comprises the steps of: performing enlargement cultivation respectively on high-temperature microbial strains such as Geobacillus, Bacillus licheniformis, two trichoderma, actinomyces and the like which are screened from the matured compost, and combining the strains in certain proportion into the compound bacterium agent. The compound bacterium agent is inoculated to the compost in certain proportion, the compost is moved into a fermentation tank for aerated aerobic fermentation, and the compost maturity is completed. Compared with the control compost, the inoculated compost of the scheme of the invention reaches the high temperature period one week earlier than the control compost, and the high temperature period lasts for 14 days so as to be beneficial to the decomposition and humification of organic matters of the compost; and the inoculated compost is thoroughly decomposed about twenty days later. Through test, most of the weed seeds, ascarid eggs and colibacillus in the compost are dead, the humic acid content is 25 g/kg higher than that of the control compost, the humification coefficient is more than 1.9, the decomposed finished product is black brown and odourless, and has excellent stability, harmless and minimized quality, apparently environmental and economic benefits.