298 results about "Actinomyces" patented technology

[Problem] To provide: a control agent having an antagonistic action on a pathogen Foc TR4 of Panama disease of Cavendish bananas; and a method for controlling the pathogen using the control agent. [Solution] The pathogen Foc TR4 of Panama disease of Cavendish bananas is controlled by using a mycelium fertilizer that is derived from a bacterium of the genus Bacillus, a heat-resistant actinomycete, or both and that is obtained by kneading 20-30 parts by weight of an organic substance having a carbon ratio of 15% or less and a water content of 25-30 parts by weight into a porous carrier having a pH of 7.5-9.5 and a particle size of 4-100 mesh.

The invention relates to the technical field of soil function improvement, in particular to a rice field soil recarburization and capacity expansion method based on a charcoal-based organic fertilizer. The method comprises the following steps: crushing a biomass raw material, pyrolyzing and cooling to obtain biochar; immersing the biochar in a functional bacteria solution containing actinomycetes and bacillus for activating treatment to obtain modified biochar; mixing the modified biochar and decomposed straw, composting and fermenting to obtain organic fertilizers with different particle sizes; the large-particle-size organic fertilizer is applied to deep soil, the soil is covered with soil after straw is laid on the soil, and the small-particle-size organic fertilizer is applied to surface soil. Through layered application, the large-particle-size biochar repairs the deep soil structure and expands the carbon storage capacity, and meanwhile, the carbon stability is improved through carbon-nitrogen synergistic metabolism driven by functional bacteria, so that the carbon immobilization efficiency is improved, meanwhile, soil recarburization and expansion are achieved, the contradiction between carbon sequestration and structure degradation and nutrient imbalance in a traditional recarburization and expansion technology is solved, and the soil recarburization and expansion efficiency is improved. Synergic improvement of a carbon sink function and agricultural productivity is promoted.

The invention discloses an organic fertilizer for promoting plant growth and improving soil and a preparation method thereof, and belongs to the technical field of organic fertilizers. The organic fertilizer is prepared from the following raw materials: composted chicken manure, soybean meal, straw powder, sweet potato vines, humic acid, a compound microbial agent, rosmarinic acid and a stimulant. The compound microbial agent is prepared from madula angustifolia actinomycetes, pseudomonas stutzeri and bacillus velezensis, and the stimulant is a glucosamine sulfate solution. The rosmarinic acid, the glucosamine sulfate solution and the compound microbial agent are added into the organic fertilizer, the three components are compounded with the straw, the chicken manure and the like to form the organic fertilizer, and various substances have a synergistic effect, so that on one hand, the growth of Chinese cabbages is promoted, and the yield is increased; on the other hand, soil physicochemical properties are obviously improved.

The invention provides actinomycetes and application thereof in prevention and control of fungal diseases of edible fungi. The actinomycetes are streptomyces cellulosae, the strain number of the actinomycetes is P8-2, and the registration number of the actinomycetes in the China General Microbiological Culture Collection Center is CGMCC No.32350, hereinafter referred to as streptomyces cellulosae CGMCC No.32350. Experimental results show that the streptomyces cellulosae CGMCC No.32350 disclosed by the invention has an inhibition effect on the fungal diseases of the edible mushrooms, including Trichoderma koningii, T.harzianum and Fusarium equiseti, and the streptomyces cellulosae CGMCC No.32350 has no influence on the growth of the edible mushrooms.

The invention relates to a mine solid waste-based composite saline-alkali soil modifier as well as a preparation method and application thereof, and belongs to the technical field of soil remediation and solid waste recycling. The modifier is prepared from volcanic rock solid waste, rice husk and mussel shell as raw materials through oxalic acid activation, microbial fermentation carbonization and humic acid chelation, and comprises 50-70 parts of activated stone powder-containing porous particles, 20-30 parts of rice husk-based charcoal, 10-20 parts of humic acid chelated magnesium-calcium powder and 3-5 parts of a composite binder. The preparation method comprises the following steps: ultrasonically activating the stone powder oxalic acid, and puffing and granulating with the binder; inoculating the rice hulls with salt-tolerant bacillus and actinomycetes for aerobic fermentation and carbonization; and the shell is activated by oxalic acid and then is loaded with a humic acid calcium magnesium compound. The modifier is applied according to 50-200kg / mu, so that the nutritional ingredients and the air permeability can be improved. According to the method, solid waste recycling, solid waste consumption and landfill cost and CO2 emission reduction are achieved, and the method is suitable for ecological restoration of the coastal saline-alkali soil and has both environmental protection and economic benefits.

The invention discloses a continuous cropping Chinese cabbage planting method based on a microbial agent and application, and belongs to the technical field of crop cultivation. According to the planting method, Chinese cabbage and garlic are intercropped according to the ratio of 1: 1, and a trichoderma harzianum agent is applied. Results show that the planting method can significantly increase the growth indexes such as the plant height, the root length, the leaf chlorophyll content and the soluble sugar content of the Chinese cabbage; the soluble protein content, the peroxidase activity, the superoxide dismutase activity and the malondialdehyde content of the Chinese cabbage leaves are obviously reduced. Meanwhile, the content of rapidly available phosphorus, rapidly available potassium, effective calcium and other substances in the soil can be increased; the activities of urease, catalase and alkaline phosphatase in the soil are also obviously improved. In addition, the number of fungi, bacteria and actinomycetes in the soil is also remarkably increased. Therefore, according to the planting method, the quality of continuous cropping soil can be improved, Chinese cabbage growth is promoted, and theoretical support is provided for healthy and sustainable development of the Chinese cabbage industry.

The invention belongs to the technical field of microorganisms, and discloses an actinomycete, which is streptomyces aureoflavus CYS4-5, is preserved in China General Microbiological Culture Collection Center (CGMCC), is preserved on October 17, 2023, and has the preservation number of CGMCC No.28506. The actinomycete has the advantages that the actinomycete can be used for preparing an actinomycete culture medium; the strain has the potential of preventing and treating salvia miltiorrhiza root rot, has obvious bacteriostatic activity on gaeumannomyces graminis, rhizoctonia cerealis, exserohilum turcicum, sclerotinia sclerotiorum, wheat root rot, fusarium graminearum and botryosphaeria dothidea, and plays an important role in biological prevention and treatment of diseases of crops such as wheat, corn, rape and grapes.

The invention provides p-hydroxymandelic acid synthetase, a gene, a mutant of the p-hydroxymandelic acid synthetase and application of the p-hydroxymandelic acid synthetase in asymmetric synthesis of (S)-mandelic acid. The p-hydroxymandelic acid synthetase disclosed by the invention is derived from actinomycetes Actinokineospora auranticola, the amino acid sequence of the p-hydroxymandelic acid synthetase is as shown in SEQ ID NO.1, and a mutant of the p-hydroxymandelic acid synthetase is obtained by performing single-point mutation or multi-point combined mutation on glutamic acid at the 187th site, isoleucine at the 189th site and threonine at the 211th site of the amino acid sequence as shown in SEQ ID NO.1. The p-hydroxymandelic acid synthetase and the mutant thereof can convert phenylpyruvic acid into (S)-mandelic acid with high optical purity, have the advantages of mild reaction conditions, simplicity and convenience in operation, safety, environment friendliness and the like, and have a very good industrial application prospect.

The invention relates to the technical field of biology, in particular to a compound microbial preparation or fertilizer for improving water quality of a mariculture pond. The composite microbial preparation comprises the following functional components in concentration: 10 < 6 >-10 < 8 > cfu / ml of bifidobacterium thermoactinomycetes, 10 < 7 >-10 < 9 > cfu / ml of rhodopseudomonas palustris, 0.03-0.06 mg / ml of dwarf vallisneria natans extract and 0.01-0.03 mg / ml of acorus calamus extract. The compound microbial preparation is clear in components and scientific in compatibility, and all the components are mutually beneficial, mutualistic and synergistic; on the premise of ensuring no pollution, no residue and no drug resistance, the content of ammonia nitrogen, phosphorus and organic pollutants in the water body of the mariculture pond can be effectively reduced; meanwhile, heavy metals, antibiotics and vibrio harveyi in the pond water body can be effectively removed, and an obvious improvement effect on the mariculture pond water body is achieved.

The invention belongs to the technical field of biological medicine and analysis, and provides an esophageal cancer early screening model based on oral microorganisms and a construction method thereof. The model is an oral cavity microbial flora as follows: Prevotella, Granulicalala, Rothia, Prevotella, Blauria, Abiotrophia, Peptostreptococcus, Actinomycetes, Burkholderia, Akkermansia, Fudania, Kineothrix, Bacillus, Duncaniella, and Eisenbergiella, and the oral cavity microbial flora is selected from the group consisting of the following microbial flora: the oral cavity microbial flora, the oral cavity microbial flora, the oral cavity microbial flora, the oral cavity microbial flora, the oral cavity microbial flora, the oral cavity microbial flora, the oral cavity microbial flora, the oral cavity microbial flora, the oral cavity microbial flora, the oral cavity microbial flora, the oral cavity microbial flora and the oral cavity microbial flora. By identifying non-invasive biomarkers, early-stage asymptomatic esophageal cancer patients can be found.

The invention discloses a high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation. According to the high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation, yield increase and quality improvement are achieved through fungus material composite strengthening treatment and a composite strain synergistic inoculation technology. The method comprises the following steps: (1) fermenting a tilia brachypoda substrate by lactobacillus plantarum and soaking in a nano-composite nutrient solution to form a high-activity bacterial material; (2) nitrogen-fixing bacteria, disease-resistant actinomycetes and armillaria mellea are mixed and inoculated according to the proportion of 3: 1: 1, nitrogen supply is improved, and the occurrence rate of black rot is reduced; 3) activating a GASA stress-resistant gene of the seed hemp through a pulsed electric field (5 kV / cm, 10 microseconds), and enabling the germination rate to reach 88.5% in combination with a calcium signal channel strengthening technology; according to the method, the yield per mu of gastrodia elata is increased by 30%-40%, the gastrodin content is increased by 30% or above, the chemical pesticide dosage is reduced by 55%, the strain passage purity is stable, the method is suitable for large-scale planting in multiple climate areas, and an innovative solution is provided for green production of traditional Chinese medicinal materials.

The invention discloses a microbial decomposing inoculant for straws and a preparation method thereof, and belongs to the technical field of microorganisms. The microbial decomposing inoculant for straw is prepared from the following raw materials in parts by weight: 15-18 parts of functional microbial liquid, 3-5 parts of neutral protease, 1-3 parts of laccase, 20-25 parts of a nano-porous diatomite carrier, 5-7 parts of a humectant, 2-3 parts of humic acid chelated trace elements and 2-4 parts of an inducer. The preparation method comprises the following steps: screening talaromyces thermophilus, Guangxi thermoactinomycetes and arthrobacter habitualis to form a functional microbial liquid, adding a laccase-protease compounding system to cooperate with the physical adsorption effect of diatomite, and adding humic acid chelated trace elements to form an ion exchange network with polyglutamic acid; straw components are efficiently degraded through a triple mechanism of'enzymatic degradation-element supplementation-microenvironment regulation and control ', the decomposition process is accelerated, and the straw compost quality is improved.

The invention provides a phytophthora nicotianae biocontrol actinomycete FK-1 and application thereof, and belongs to the technical field of biocontrol bacteria. The invention provides a streptomyces strain FK-1, and the preservation number of the streptomyces strain FK-1 is CCTCC (China Center for Type Culture Collection) NO: M 20242872. The streptomyces FK-1 disclosed by the invention shows remarkable broad-spectrum antibacterial activity on various phytopathogens, and has the capabilities of slowing down pathogen infection and reducing plant diseases. The inhibition rate of the streptomyces FK-1 to phytophthora nicotianae reaches 82.08%, the antibacterial activity of strain fermentation liquor can reach 85.88%, and the prevention and treatment effect of indoor potting is 80.65%; meanwhile, the phytophthora nicotianae hyphae can be deformed, wound and irregularly bent, so that the growth of the phytophthora nicotianae hyphae is inhibited, and the method can be applied to prevention and control of phytophthora nicotianae.

The invention relates to the technical field of microbial medicines, in particular to a siderophore compound (hydroxamic acid salt siderophore compound) and a preparation method and application thereof. The compounds are the following compounds 1-3; the hydroxamic acid salt siderophore provided by the invention has remarkable iron chelating ability, promotes seed germination and plant growth activity, and can be used as an ideal compound of an iron overload disease therapeutic agent and a growth regulator for promoting crop growth. The compound can be produced through fermentation of special actinomycetes, the preparation method is simple and efficient, and the obtained product is high in purity. The method has a good application prospect. # imgabs0 #

The present invention belongs to the field of microbial technology and specifically relates to a salt-tolerant growth-promoting composite bacterial agent and its application. The active ingredients of the salt-tolerant growth-promoting composite bacterial agent are actinomycete S36, deposited with CGMCC No. 32928, Trichoderma M2, deposited with CGMCC No. 41651, and Bacillus velezensis Y10, deposited with CGMCC No. 32927. The present invention screened and obtained a composite bacterial agent obtained by mixing these three bacteria, which can improve the resistance of cotton and wheat to salt stress, providing a new microbial resource for improving the salt tolerance of cotton and wheat.

The invention discloses a method for preparing a biological bacterial fertilizer from coal and microorganisms, and belongs to the technical field of biological fertilizers. The method comprises the following steps: crushing coal to 80 meshes to obtain coal powder; adding a compound fertilizer accounting for 3% of the total fertilizer mass; a composite additive accounting for 1% of the total mass of the fertilizer is added, and the additive comprises 95% of mushroom sticks, 3% of glucose and 2% of microbial flora (bacillus subtilis, actinomycetes, lactic acid bacteria and the like are mixed according to the proportion of 1: 1: 1: 1); the raw materials are mixed and granulated. The organic matter content of the prepared biological bacterial fertilizer is larger than or equal to 30%, the effective viable count is larger than or equal to 2 * 10 < 7 > CFU / g, the heavy metal content is remarkably lower than the national standard, and cadmium is smaller than or equal to 0.5 mg / kg, lead is smaller than or equal to 30 mg / kg, and chromium is smaller than or equal to 80 mg / The method realizes efficient biotransformation of coal resources, and is suitable for soil improvement and organic agricultural production.

The invention discloses a bifidobacterium animalis subsp. Lactis BA-C53 and an application of the bifidobacterium animalis subsp. Lactis BA-C53 in products for improving bone health. The bifidobacterium animalis subsp. Lactis BA-C53 is classified and named as bifidobacterium animalis subsp. Lactis Bifidobacterium animalis subsp. Lactis BA-C53, the preservation number is CCTCC NO: M 2024460, the preservation unit is China Center for Type Culture Collection, and the preservation time is March 11, 2024. The bifidobacterium animalis subsp. Lactis BA-C53 can effectively improve the steady state of intestinal flora of a mouse with osteoporosis induced by estrogen deficiency, so that the relative abundance of phylum firmicalis and phylum actinomycetes is increased, the relative abundance of phylum bacteroides is reduced, and then short-chain fatty acid is generated to activate intestinal immunity and maintain the integrity of an intestinal barrier, so that the osteoporosis caused by estrogen deficiency is inhibited. Meanwhile, osteoclast differentiation can be inhibited by regulating NFATC1, RANKL and OPG pathways, then osteoporosis is relieved, and the compound can be widely applied to preparation of food and drugs.

The invention relates to the technical field of microbial pesticides and microbial fertilizers, in particular to actinomycetes antagonizing various plant pathogens and application of the actinomycetes antagonizing various plant pathogens, and provides a YC3041 strain with the taxonomic name of Streptomyces pratensis and the preservation number of GDMCC No: 64828. The technical scheme provided by the invention has the characteristics of low use cost, good control effect, no residue and safety to human, livestock and environment, and the actinomycetes antagonizing various plant pathogens and the application of the actinomycetes antagonizing various plant pathogens have broad application prospects. The biological pesticide prepared from the YC3041 strain has the control effect of 70% or above on tobacco root knot nematode disease, tobacco bacterial wilt, tobacco black shank and compound diseases caused by the tobacco root knot nematode disease, the tobacco bacterial wilt and the tobacco black shank.

The invention provides compost and a preparation method thereof, and relates to the technical field of organic fertilizers, the compost comprises the following components by weight: 900-1100 parts of crop straw, 200-300 parts of animal excreta, 100-200 parts of an organic auxiliary material, and 1.5-7.5 parts of a complex microbial inoculant; the complex microbial inoculants comprise lactic acid bacteria, bacillus licheniformis, thermophilic trichoderma, actinomycetes and white rot fungi. The preparation method of the compost mainly comprises the following steps: stacking the crop straws and the organic auxiliary materials layer by layer, and compacting; uniformly sprinkling animal excrement on each layer, and then inoculating a complex microbial inoculant; punching a hole in the top of the pile to monitor the moisture content in the pile in the composting process; pile turning treatment is carried out in the composting process. According to the invention, the complex microbial inoculants are adopted for compost maturing treatment, so that the maturing speed can be increased, the maturing effect can be improved, and the content of nutrient substances in compost is increased.

The invention discloses a compound microbial agent as well as a preparation method and application thereof, and belongs to the technical field of agricultural waste rapid composting microbial agents. The prepared composite microbial agent comprises bacillus siamensis, bacillus megatherium, azotobacter chroococcum, bacillus subtilis, bacillus amyloliquefaciens, actinomycetes, saccharomycetes and lactobacillus plantarum, and is used in the composting process of compatibility of pig manure and sorghum straw. Substances such as protein, starch, cellulose and lignin in the raw material pig manure and crop straw can be decomposed, and the degradation speed and effect of compost are improved; the composting quality can be improved, harmful substances are reduced, and the composting is safer and more efficient. The compound microbial agent has excellent performance in actual use, and can provide high-quality organic fertilizer for agricultural production.

The invention discloses a preparation method of an actinomycetes and waste mushroom matrix composite, belongs to the technical field of environmental microorganisms, solves the problems of low resource utilization rate, serious environmental pollution and the like in the existing waste mushroom matrix treatment, and overcomes the defects of loose soil structure and poor erosion resistance caused by direct returning of the waste mushroom matrix to the field. The method comprises the following steps: separating actinomycetes strains from soil, purifying and culturing the actinomycetes strains into a bacterial solution, crushing a waste mushroom substrate, adjusting the water content and the initial pH value, inoculating the bacterial solution into a pretreated substrate, and culturing in a dark place to form a composite material with biological activity and structural stability. Lignocellulose in a matrix is efficiently degraded by utilizing cellulase secreted by actinomycetes and lignin peroxidase, and a three-dimensional cross-linked structure is formed through a hypha network and humic acid-minerals, so that the mineralization efficiency of organic matters and soil agglomeration are improved. The composite material can be applied to the field of water and soil loss prevention and control, and efficient conversion of waste resources and collaborative improvement of soil ecological functions are achieved.

The invention relates to a formulation of microbial consortia selected from (a) Bacilli group, (b) Pseudomonas group and (c) Actinomycete group having nitrogen fixing capacity, when the bacteria comes in contact with soil or any aqueous media it gets active, which further helps for plant growth and makes the soil environment more suitable for plant.

The invention provides a konjac continuous cropping modifier based on rhizosphere microflora regulation and control, and relates to the technical field of plant planting reagents, the modifier comprises the following components: actinomycetes, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, disease-resistant fungi, humic acid and seaweed extract, an organic carrier and a mineral supplement, microbial strains with different functions such as actinomycetes, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria and disease-resistant fungi can be colonized in soil, and nitrogen, phosphorus and potassium elements in the soil are decomposed by inhibiting harmful pathogenic bacteria, so that the nutrient structure of the soil is improved, and the biological activity of the soil is enhanced. The synergistic effect of microorganisms effectively enhances the disease resistance of the soil and solves the problem of continuous cropping soil deterioration.

The invention relates to the field of microorganisms, and discloses application of lactococcus lactis in a product for improving sleep. The lactococcus lactis is named as WHH2078, is separated from yoghurt butter collected from a country in Sankarst city in Tibet Autonomous Region in China, is preserved in China General Microbiological Culture Collection Center on February 25, 2021, has a preservation number of CGMCCNO.21831, and is classified and named as Lactococcus lactis. The Lactococcus lactis is named as Lactococcus lactis, and the Lactococcus lactis is named as Lactococcus lactis. The lactococcus lactis has the effects of shortening the sleep latency, prolonging the total sleep time, improving 5-hydroxytryptamine and gamma-aminobutyric acid of the brain, serum and colon, lowering the corticosterone level, regulating back the diversity of intestinal microorganisms alpha, regulating up the abundance of intestinal acidobacilli and lowering the abundance of actinomycetes, desulfurization bacilli and verruca microflora. The sleep disorder can be effectively improved.

The present invention discloses an organic fertilizer for promoting plant growth and soil improvement and a preparation method thereof, and belongs to the technical field of organic fertilizers. The organic fertilizer comprises the following raw materials: decomposed chicken manure, soybean meal, straw powder, sweet potato vine, humic acid, composite microbial agent, rosmarinic acid, and a stimulant. The composite microbial agent is composed of Actinomyces millefolius, Pseudomonas stutzeri, and Bacillus velezensis, and the stimulant is a glucosamine sulfate solution. Rosmarinic acid, glucosamine sulfate solution, and composite microbial agent are added to the organic fertilizer of the present invention. These three components are compounded with straw, chicken manure, etc. to form an organic fertilizer. The synergistic effect of multiple substances promotes the growth of cabbage and increases yield on the one hand; on the other hand, it significantly improves the physical and chemical properties of the soil.

The invention provides a pill-type organic fertilizer special for vegetables. The pill-type organic fertilizer is prepared from the following raw materials in parts by weight: modified biochar, fermented livestock and poultry manure, plant source protein zymolyte, a complex microbial inoculant, a trace element supplement and a binder, and a method therefor; the complex microbial inoculants comprise actinomycetes, bacillus amyloliquefaciens and trichoderma harzianum; the trace element supplement comprises EDTA (Ethylene Diamine Tetraacetic Acid) chelated zinc, EDTA chelated iron and boric acid. Therefore, on one hand, the organic fertilizer can effectively improve the facility agriculture soil environment, adjust the soil acidity and alkalinity, reduce the salinization degree, promote balance and stability of microbial communities and restore and improve the ecological function of soil; on the other hand, the utilization rate of the fertilizer can be greatly improved, and the plant-source proteolysis provides a quick-acting nitrogen source to promote root development; the microelement supplement meets the requirements of vegetables on microelements; nutrients are balanced, vegetable stress resistance is enhanced, and photosynthetic efficiency is improved; and the binder enables the fertilizer to form uniform particles, is suitable for mechanical fertilization, and improves the fertilization efficiency.

The invention discloses a microbial agent for increasing manure compost temperature and application thereof in high-temperature compost, and belongs to the field of agricultural solid waste treatment. The microbial agent for increasing the manure composting temperature comprises phanerochaete chrysosporium, bacillus stearothermophilus, penicillium oxalicum, streptomyces, common thermoactinomycetes and bacillus subtilis. The livestock padding manure high-temperature clean composting technology is established through adjustment and optimization, the purposes that the temperature of the padding manure is increased fast, the high-temperature period is long, the composting period is shortened, base materials are fully degraded are achieved, reference can be provided for resource utilization of livestock and poultry breeding solid waste, and important application value is achieved.

The invention discloses a polysaccharide polymer, application of the polysaccharide polymer and a preparation method of the polysaccharide polymer. The polysaccharide polymer QPS capable of remarkably selectively inhibiting harmful bacteria in aquaculture is separated and purified from the water mold antagonistic actinomycete strain QHV2 for the first time, can effectively inhibit mycelial growth and spore germination of water mold / branchiomycosis and destroy mature biological membranes, is stable in activity, free of toxic effects on fry and good in biological safety, and can be applied to the field of aquaculture. The material can be used as a candidate material for research and development of a fish pathogenic fungus biological prevention and control agent. Meanwhile, the polysaccharide polymer QPS avoids pollution of harmful substances to a water body when a QHV2 fermentation product is directly applied, can selectively inhibit staphylococcus aureus, saprolegnia, branchiomycosis and other harmful bacteria in aquaculture, and has no inhibition or weak inhibition on pseudomonas pseudoalcaligenes, bacillus and other beneficial bacteria in aquaculture. And the application effect is better.

The disclosure belongs to the technical field of biomedicine and analysis, and provides an early screening model for esophageal cancer based on oral microbiota and a construction method therefor. The model uses the following oral microbiota as markers: Prevotella, Granulicatella, Rothia, Prevotellamassilia, Blautia, Abiotrophia, Peptostreptococcus, Actinomyces, Burkholderia, Akkermansia, Fudania, Kineothrix, Bacillus, Duncaniella, and Eisenbergiella. The disclosure detects early asymptomatic esophageal cancer patients by identifying non-invasive biomarkers.

The invention belongs to the field of microbial agents, and particularly relates to a compost heat production method of a ganoderma lucidum basswood planting waste substrate, which comprises the following steps: mixing the ganoderma lucidum basswood planting waste substrate with chicken manure to obtain a fermentation raw material; paving the fermentation raw materials in a fermentation container layer by layer; a complex microbial inoculant is uniformly sprayed on the surface of each layer of fermentation raw material; the complex microbial inoculant is prepared from the following strains in percentage by weight: 10 to 25 percent of bacillus cereus, 5 to 10 percent of bacillus smith, 5 to 10 percent of geobacillus thermodenitrificans, 20 to 40 percent of bacillus licheniformis, 5 to 24 percent of ureibacillus thermophilus and the balance of actinomyces thermophilus; the oxygen content is controlled to be 5-15%; the 50-60 DEG C high-temperature period is maintained for 180-210 days, the heat exchange pipe located between the two layers of fermentation raw materials is connected to a heating system in the high-temperature period, and the system is suitable for agricultural circular economy and distributed energy supply scenes.