197 results about "Quorum sensing" patented technology

The invention discloses bacillus (Bacillus sp.) GB-1. The preservation number of the bacillus (Bacillus sp.) GB-1 in Guangdong Microbial Culture Collection Center is GDMCC NO: 65601. The bacillus (Bacillus sp. The strain GB-1 has a good effect of degrading quorum sensing signal molecules OHHL, the quorum quenching effect of the strain is stable and remarkable, a new way and method are provided for biological control of AHLs-mediated pathogenic bacteria, the strain has huge application potential, the use of chemical pesticides can be reduced, and the cost is reduced. A new way is provided for replacing chemical prevention and control with biological prevention and control, and the application prospect is good.

The invention belongs to the field of microbial preparations, and particularly relates to a complex microbial inoculant for promoting microbial quorum sensing and application of the complex microbial inoculant in compost. The composite microbial agent comprises pseudomonas A3 and paenibacillus polymyxa 21-2, and the preservation number of the pseudomonas A3 is CCTCC (China Center for Type Culture Collection) NO: M20251394. The pseudomonas A3 can express key genes such as rhlI and lasI of a gram-negative bacteria AHL quorum sensing system and signal molecules of the key genes, and the paenibacillus polymyxa 21-2 can express luxS genes of a gram-positive bacteria AI-2 signal system and signal molecules DPD of the luxS genes. In the pig manure compost, the complex microbial inoculant is added, so that the expression of AI-2 and AHLs system related genes in the compost and the content of signal molecules DPD are remarkably improved, the microbial population sensing process of the compost is promoted, finally, the compost temperature is increased by about 6 DEG C, the compost carbon-nitrogen ratio is reduced, and the compost maturity is promoted.

The invention relates to a quorum sensing intelligent regulation and control method for violaceum violaceum in an antibiotic gradient region, and aims to solve the problems of uncontrollable multi-partition microenvironment and low biological behavior consistency in an existing system, the quorum sensing intelligent regulation and control method for the violaceum violaceum in the antibiotic gradient region comprises the following steps: accurately positioning space partitions, and collecting liquid phase pH, oxygen concentration and nutrient components in each region in real time by an integrated sensor; high-throughput detection and multi-modal data fusion are combined, and an AI model is input to establish association mapping of a liquid / gas phase environment and a biological phenotype. The system automatically generates and optimizes a partition gas component adjustment instruction, realizes spatial synchronous gas phase regulation and control by utilizing a programmable micro-airflow device, and realizes multi-region environment dynamic balance regulation and control in a culture chamber.

The invention discloses a method for cooperatively controlling biological pollution of a reverse osmosis membrane by group quenching bacteria and bacteriophages. According to the invention, a QQ-bacteriophage carrying system composed of quorum quenching (QQ) bacteria and lysing bacteriophages is constructed, and the bacteriophages and the QQ bacteria are utilized to synergistically improve the inhibition effect on biofilm formation and biological pollution. In the QQ-bacteriophage carrying system, the bacteriophage can perform targeted lysis on core contaminating bacteria, and the QQ bacteria can degrade quorum sensing molecules, weaken an anti-bacteriophage defense system of the bacteria and promote movement and infection of the bacteriophage in a biological membrane. Therefore, the QQ-bacteriophage carrying system can remarkably improve the biological membrane removal efficiency, recover the membrane flux and prolong the service life of the reverse osmosis membrane. The invention can provide an efficient, green and sustainable biological pollution control solution for the fields of water treatment, seawater desalination, industrial wastewater reuse and the like.

The invention relates to the technical field of biologic stimulant preparation, and discloses a biologic stimulant mixing preparation two-stage control method, which comprises: adopting dissolved oxygen gradient and carbon source pulse cooperative control, linearly reducing the concentration of dissolved oxygen in a fermentation tank from an initial value at a specific rate, and injecting a carbon source at regular time; when the flora density and the quorum sensing signal molecule concentration reach specific threshold values, triggering stage switching; starting pressure-metabolism coupling control, increasing the pressure of the fermentation tank at a specific rate, and adding coenzyme Q10; the quorum sensing inhibitory peptide containing a specific core structure is synchronously released in the boosting process; after the pressure is stable, a real-time mapping relation between a microbial metabolism state and physical parameters is constructed in a thallus proliferation stage through a coupling mechanism of dissolved oxygen gradient control and quorum sensing signal monitoring, and the industrial problem that a traditional static threshold value cannot respond to pressure fluctuation of a fermentation tank to cause signal attenuation is solved.

The invention provides a dynamic control system for coordinating streptomyces secondary metabolite production and application of the dynamic control system. Firstly, a general artificial promoter PQS (trigger) responding to a quorum sensing system of various streptomycetes is designed, then a stabilizer is constructed based on a bistable gene circuit, instantaneous signal output is converted into stable signal output, dependence of an inducer is successfully eliminated, finally, an orthogonal amplifier is developed based on a streptomycete rare extracellular function sigma factor, and finally, the specific function of the quorum sensing system of various streptomycetes is achieved. And different promoter element libraries containing amplifiers with response from weak to strong and with strength span of about 535 times are constructed. An amplifier and a CRISPRi system are combined to construct a turner, and gene expression inhibition with the strength span of about 200 times is achieved. The combination of the amplifier, the promoter library and the turner is called a multi-effect device, and abundant elements are provided for fine target gene expression. The combination of the trigger, the stabilizer and the multiple-effect device is called as a streptomycete multipath manual control system (SMARTS), and control tools of streptomycete are enriched.

The invention discloses a quorum sensing inhibitor loaded nano composite material and a preparation method and application thereof, and belongs to the field of biomedical materials.The preparation method comprises the steps that MnO2 nano particles are dispersed in a polyallylamine hydrochloride aqueous solution, and a MnO2 / PAH compound is obtained; dispersing the MnO2 / PAH compound in a polyacrylic acid aqueous solution for secondary modification; the modified nano particles are mixed with a MgCl2 solution, ultrasonic treatment and aging treatment are carried out, and a MnO2-Mg compound is obtained; platinum nanoparticles grow in situ and are loaded on the surface of the MnO2-Mg compound by adopting a chemical reduction method to obtain MnO2-Mg / Pt; and loading the BBF on the MnO2-Mg / Pt to obtain the MnO2-Mg / Pt-coated BBF nano composite material. The invention aims to enhance the chemical kinetics efficiency and cooperate with the biomembrane inhibition effect to realize efficient treatment of periodontitis.

The invention relates to the field of rice seedling raising cultivation, and discloses a preparation method of a pest-resistant orderly-released rice seedling raising substrate, which comprises the following steps: (a) preparing a composite microcapsule which comprises a functional microorganism core and an intelligent response type shell coating the functional microorganism core; wherein a compound microbial flora and a quorum sensing signal precursor are loaded in the functionalized microbial core; the intelligent response type shell contains a plant immunity inducer, and the material of the shell is set to be degraded under the action of rice root exudates. By designing a composite microcapsule structure, programmed and targeted release of active substances is realized, so that a plant immunity inducer acts in advance in the early stage of seedling raising, and a composite microbial flora is accurately released to a rhizosphere area only when a rice root system is in contact with the microcapsule, so that the effect of raising seedlings is achieved. And the utilization efficiency and the action accuracy of each functional component are obviously improved.

The invention relates to the technical field of soil fertility management, in particular to a production quality control method of a special fertilizer for Yunnan cigar tobacco leaves, which comprises the following steps: step 1, preparing a waste-based functional carrier; step 2, signal response type fermentation control: based on a dynamic monitoring result of microbial population sensing signal molecules in a fermentation system, regulating and controlling the injection opportunity of an exogenous inducer and fermentation environment parameters, and directionally synthesizing a targeted antibacterial metabolite; step 3, adaptive packaging in a growing period; and 4, bionic verification and closed-loop optimization: verifying a nutrient release curve through a bionic root system simulation device, and feeding back and adjusting packaging parameters in combination with a field metabolite detection result to form a production-application self-optimization system. The production efficiency and fertilizer efficiency of the cigar tobacco leaf fertilizer are improved through accurate process control, it is ensured that nutrient supply and release of the fertilizer in all growth periods have excellent matching performance, and the effect of the fertilizer can be optimized in practical application.

The invention discloses a method for culturing low-temperature aerobic granular sludge, and relates to the technical field of sewage treatment, and the method adopts a high-concentration dissolved oxygen (DO) aeration and long-period operation mode to improve the tolerance and adhesion capacity of microorganisms to a low-temperature environment by enhancing the carbon metabolism capacity and extracellular polymeric substance secretion of the microorganisms. Meanwhile, the operation mode of combining high aeration and mechanical stirring not only can generate larger shearing force to remove loose biological membranes on the surface of the sludge, but also can enhance the aggregation ability of the microorganisms by promoting quorum sensing phenomena among fimbriae, flagellum and the microorganisms. The intermittent operation mode creates a satiation-starvation environment for the growth of microorganisms, and further enhances the stability of the particles. Along with shortening of the operation cycle, the system can further complete particle screening, and the low-temperature-resistant aerobic granular sludge with high settleability is reserved.

The invention relates to a method for controlling biological pollution of a reverse osmosis membrane through photoelectrochemical filtration pretreatment. According to the method, a photoelectrochemical reactor is mainly established to inhibit the bacterial biofilm forming ability under ultraviolet illumination and voltage conditions. The photoelectric chemical reactor is composed of a peristaltic pump, a magnetic stirrer, a reactor, an electrochemical filtering assembly, a direct-current power source and an ultraviolet light source and inhibits bacteria added into the reactor from forming a biological membrane. Under the action of voltage, the electrochemical filter assembly inhibits the biofilm forming ability and viability of bacteria by degrading protein and quorum sensing signal molecules in water, and more thoroughly inhibits the biofilm formation through the irradiation of ultraviolet light. Therefore, according to the method disclosed by the invention, the ultraviolet light can be combined with the electrochemical filter assembly to realize effective inhibition of a biological membrane formed by bacteria, the reduction of membrane flux is reduced, and the method has important significance in controlling biological pollution of the reverse osmosis membrane.

The invention discloses a biological agent for preventing and treating panax notoginseng root rot, and relates to the technical field of biological prevention and treatment, the biological agent is composed of four layers of functional florae: an A layer of sentinel-decoding flora is used for identifying a pathogen quorum sensing signal and is used as an event trigger source, a B layer of metabolic countering flora is used for decoupling conversion of disease-promoting saponin and controllable release of a metabolic false substrate, and a C layer of functional florae is used for controlling root rot of panax notoginseng. The C-layer self-healing conductive biological membrane construction flora forms a biological membrane with conductivity and self-healing capability on the root surface, and the D-layer electrically-enabled flora constructs stable biological micro-current at the rhizosphere by using ferric oxide particles as external electron acceptors. The layer A triggers to cause controlled release of the layer B, a conversion product of the layer B provides a carbon source and a structural unit for the layer C, and a conductive network of the layer C and the layer D form an electronic path. The biological agent is used for early recognition and continuous inhibition of pathogens in panax notoginseng planting soil, can start defense response in the early stage of quorum sensing signal occurrence, and keeps action stability under complex environmental conditions.

The invention discloses a method for improving yield of branched chain aldehyde of lactococcus lactis based on regulation and control of a luxS gene and application, and belongs to the field of system biology. Gene function verification and genome scale metabolic network model analysis are creatively combined, the phenotypic effect of the luxS gene is confirmed, and more importantly, the internal action mechanism of the luxS gene is disclosed from the system level, that is, the whole metabolic network flow is influenced by regulating and controlling multiple key enzymes such as transaminase, decarboxylase, dehydrogenase and decarboxylase. The invention reveals that the quorum sensing core gene luxS has a new function of regulating and controlling synthesis of branched chain aldehyde in lactococcus lactis for the first time, and an intercellular communication system is directly linked with production of specific flavor metabolites. The invention provides a brand-new and higher-level regulation target and thought for producing flavor substances by microbial fermentation, and gets rid of the limitation that only metabolic pathway terminal enzyme is singly modified traditionally.

The invention belongs to the technical field of biological medicine, and particularly relates to application of 4-imidazole formaldehyde in a pseudomonas aeruginosa quorum sensing inhibitor. It is found for the first time that 4-imidazole formaldehyde can inhibit quorum sensing of pseudomonas aeruginosa, reduce QS system regulation gene expression and virulence factor yield and remarkably reduce infection caused by QS system regulation gene expression and virulence factor yield; the drug application designed on the basis of bacterial metabolism can avoid the drug resistance of a conventional bacteriostatic agent, and a new strategy is provided for prevention and treatment of pseudomonas aeruginosa infection.

The invention discloses an application of bilobalide in preparation of a product for resisting salmonella typhimurium infection. The bilobalide takes salmonella typhimurium QseB protein as a target, has a good interference effect on an AI-2 quorum sensing signal of salmonella typhimurium and has good pharmacological activity, and particularly, the bilobalide has good pharmacological activity under the condition that the growth of salmonella typhimurium is not inhibited. The motility, biofilm formation and infection pathogenicity of salmonella typhimurium are obviously inhibited. The bilobalide does not influence the growth of salmonella typhimurium, directly acts on pathogenic factors, weakens the pathogenicity of bacteria from the source, does not generate selective pressure on salmonella typhimurium, and does not cause the generation of drug-resistant pathogenic bacteria. Therefore, the bilobalide has a good application prospect in the aspect of development of the salmonella typhimurium infection resisting medicine.

The application discloses a Vibrio parahaemolyticus quorum sensing inhibiting peptide and application thereof, and belongs to the field of bioactive peptides. The inhibiting peptide comprises the following amino acid sequence: Ser-Phe; the application further discloses application of the quorum sensing inhibiting peptide in inhibiting Vibrio parahaemolyticus or preparing antibacterial drugs for inhibiting Vibrio parahaemolyticus. The application adopts a molecular docking technology to screen an active peptide having an inhibiting effect on a Vibrio parahaemolyticus LuxS / AI-2 quorum sensing system, ADMET property prediction is carried out on a peptide segment with better binding force, and finally, a bioactive peptide SF stably combined with LuxP, LuxQ and LuxS is obtained; the dipeptide has good in-vitro inhibiting activity on the Vibrio parahaemolyticus LuxS / AI-2 quorum sensing, and has no cytotoxicity. The dipeptide obtained by the application can be used for guiding development of a new type of antibacterial preparation applied to food, medicine and agriculture and other fields, and has great significance for human health and agricultural development.

This invention discloses the application of δ-juniperene in the preparation of quenchers that inhibit the quorum sensing system of *Serratia liquefaction*. It belongs to the field of pharmaceutical technology. The purpose of this invention is to provide a quencher for inhibiting the quorum sensing system of *Serratia liquefaction*, thereby reducing the toxicity of pathogens without inhibiting their growth. This invention provides an application of δ-juniperene in drugs that inhibit the quorum sensing system of *Serratia liquefaction*, offering a novel solution for developing highly efficient and safe food preservatives.

The invention discloses equipment and a method for intensively treating sewage based on quorum sensing. The equipment comprises a main body supporting mechanism, and a sewage purification mechanism, a microorganism monitoring mechanism and a microorganism culture mechanism which are arranged in the main body supporting mechanism, the main body supporting mechanism comprises a main body supporting outer shell; a main body supporting inner shell is fixed at the bottom in the main body supporting outer shell; an annular sewage treatment ring channel is formed between the inner side wall of the main body supporting outer shell and the outer side wall of the main body supporting inner shell; the sewage purification mechanism comprises a purification mechanism support frame fixed at the bottom in the sewage treatment ring channel, a plurality of sewage purification circulation shells with two through ends are fixed on the purification mechanism support frame, and a plurality of microorganism attachment plates are fixed in the sewage purification circulation shells; the quorum sensing signal monitoring system has accurate quorum sensing signal monitoring capability, the quorum sensing signal sensors distributed in a scattered mode can accurately monitor signals, monitoring results of the quorum sensing signal sensors can be mutually compensated and corrected, and the accuracy of data is ensured.

Described herein is a synergistic combination comprising a quorum-sensing inhibitor and / or postbiotic metabolite and an antibiotic. Typically, the postbiotic metabolite comprises at least one peptide. Related compositions, uses, and methods are also described, including methods for resensitizing resistant bacteria to an antibiotic, and methods of treating antibiotic-resistant infections, such as methicillin-resistant Staphylococcus aureus (MRSA).

Provided is an engineered bacterium for inhibiting the synthesis of dodecanedioic acid. The expression of a related redox gene is knocked out or down-regulated in the genome of the engineered bacterium. The engineered bacterium can effectively reduce the production of the by-product dodecanedioic acid and prevent the peroxidation of 12-hydroxydodecanoic acid during the synthesis of nylon 12 monomer. Further provided is a method for constructing an engineered bacterium for inhibiting the synthesis of dodecanedioic acid. The method involves synthesizing nylon 12 monomer in Escherichia coli by using a quorum-sensing system in Vibrio fischeri. The established Escherichia coli containing a self-induction system can produce the nylon 12 monomer from glucose, and the generation of the by-product dodecanedioic acid can be effectively reduced. After fermentation in a shake flask for 72 h, the yield of the nylon 12 monomer is more than 100 mg / L, and the yield of the by-product dodecanedioic acid is reduced to less than 200 mg / L (the proportion in the product is less than 20%).

The invention discloses a piglet anti-diarrhea complete feed formula based on a microecological preparation, and relates to the technical field of animal nutrition and health. The microsphere has a unique three-layer core-shell structure, wherein a core layer is coated with butyric acid producing probiotics and precise prebiotics; the middle layer is a chitosan-gallic acid-vanillin terpolymer with dual functions of strong mucous membrane adsorbability and quorum sensing quenching; and the shell layer is a pH-sensitive slow-release coating and is loaded with an acidifying agent. The structure is constructed through a layer-by-layer coating process, and the technical problems that in the prior art, functional components cannot be delivered in a targeted mode, the synergistic effect is poor, and intestinal barriers cannot be actively repaired are solved. When the microspheres enter animal digestive tracts, the shell layer firstly releases an acidifying agent at the front end to inhibit pathogenic bacteria and clear obstacles for subsequent steps; after entering the small intestine, the middle layer polymer is released, adheres and repairs the damaged intestinal mucosa in the form of a biological bandage, and interferes pathogen communication at the same time.

The present invention relates to the preparation and use of high-entropy nanozymes with multi-pathway synergistic antibacterial effects, belonging to the field of nanozyme catalytic materials. The present invention, for the first time, utilizes a hydrothermal method and high-temperature calcination method to synthesize oxygen-vacancy-rich high-entropy oxides with haloperoxidase-like, peroxidase-like, and glutathione oxidase-like activities. These oxides can catalyze bromide ions and hydrogen peroxide to generate hypobromous acid, which interferes with bacterial quorum sensing, and hydroxyl radicals, which disrupt structure and function. Furthermore, these oxides consume glutathione, which maintains the internal redox balance of bacteria. This allows for multi-pathway synergistic and efficient bacterial damage and inactivation, helping to address the problems of existing antifouling nanozyme materials, which suffer from a single catalytic pathway and poor component adjustability. This allows for the practical expansion of high-entropy nanozyme coatings, and has potential application prospects in the field of marine biofouling.

The invention relates to the technical field of analysis, and discloses a method for quantifying AHLs in anaerobic leavening and activated sludge based on LC-MSMS (Liquid Chromatography-Mass Spectrometry-Mass Spectrometry). The method comprises the following steps: sequentially extracting, purifying and extracting a to-be-detected object to obtain a to-be-detected solution; and then preparing a standard substance: setting reasonable chromatographic conditions and mass spectrum conditions, and finally calculating the content of AHLs. Compared with an existing method, the method has the advantages of being more thorough in extraction, higher in sensitivity, lower in detection limit, high in recovery rate, easy to operate and the like, and is suitable for two matrixes of activated sludge and anaerobic fermentation products. A new method and thought are provided for studying the quorum sensing effect of AHLs of activated sludge and anaerobic fermentation products, and a brand new quality control means can be provided for sewage treatment plants and biogas projects.

The invention relates to application of extracellular vesicles in antibacterial yoga clothes, the application comprises that the extracellular vesicles are micro-fixed on the surface layer of yoga clothes fabric through a padding method or a spraying method, and the problems that chemical antibacterial agents (silver ions, graphene and the like) of existing yoga clothes are high in cost and large in skin irritation, and environment / health risks exist are solved. The extracellular vesicles are slightly fixed on the surface layer of the yoga clothes fabric through a padding method or a spraying method, and the extracellular vesicles can inhibit biological membranes formed by pathogenic bacteria such as staphylococcus aureus and escherichia coli on the surface layer of the yoga clothes fabric by interfering pathogenic bacteria quorum sensing. The fabric has the advantages that peculiar smell substances such as sulfides and fatty acid volatile substances generated by metabolism of bacteria in a sweat wet environment are reduced from the source, fabric peculiar smell control is achieved, compared with chemical antibacterial agents such as silver ions and graphene, biotoxicity risks and environmental accumulation hidden dangers are avoided, anaphylactic reactions caused by long-term contact between yoga clothes and skin can be remarkably reduced, and wearing safety is improved.

The present invention belongs to the field of pharmaceutical technology and relates to a preparation that binds to the AbaR protein of Acinetobacter baumannii and its application. The present invention utilizes sansinol O to effectively bind to the AbaR protein in Acinetobacter baumannii, thereby inhibiting its AHL quorum sensing system. It has been confirmed that sansinol O can significantly inhibit the biofilm formation ability of Acinetobacter baumannii ATCC 19606 strain, reduce bacterial motility, and reduce bacterial toxicity to cells, without significantly affecting bacterial growth and causing selective pressure to promote bacterial evolution of drug resistance.

This invention belongs to the fields of genetic engineering and microbial technology, specifically relating to a yeast quorum sensing dynamic regulation system, method, and its applications. This invention successfully constructs a functional, bacterial-derived RpaI / RpaR quorum sensing system in yeast for the first time. The constructed yeast quorum sensing dynamic regulation system possesses advantages such as low background leakage, high sensitivity, and wide dynamic range. By flexibly coupling the core system with a signal amplification module (Gal4) and a signal conversion module (CRISPRi), a bifunctional cascade platform integrating activation and inhibition is constructed, achieving multidimensional and precise dynamic regulation of metabolic pathways. Furthermore, the system has been applied to the biosynthesis of several important compounds, including cordycepin, geraniol, and 3-hydroxypropionic acid, significantly increasing yields and effectively balancing the conflict between cell growth and product synthesis, demonstrating its great potential as a universal dynamic regulation tool.

The invention relates to the technical field of biotechnology, enzyme engineering and biological pharmacy, in particular to a ribonuclease purification production method. According to the method, a deep sea antifreeze polypeptide and rare earth nanocrystal composite bionic system is constructed, quorum sensing signal molecules and a pulsed electric field are introduced in a fermentation stage for two-factor regulation, and a light-operated micro-fluidic chip selective extraction technology is combined, so that efficient separation of target enzyme is realized. A double-ligand magnetic microsphere chromatographic column is innovatively adopted for gradient elution, and the enzyme activity retention rate and the long-term storage stability are remarkably improved through thermotropic liquid crystal capsule stabilization and radio frequency assisted freeze drying processes. According to the invention, the destructiveness of the traditional chromatography technology to enzyme conformation is broken through, the problem of batch fluctuation caused by multi-step purification is solved, a ribonuclease product with high purity, high activity and environmental response characteristic is provided for the field of biological medicine, and the ribonuclease is especially suitable for industrial preparation of gene therapy products and virus vectors.

The invention relates to the technical field of agricultural biology, and discloses a high-yield wheat planting method which comprises the following steps: S1, screening and compounding nitrogen-fixing bacteria, phosphate-solubilizing bacteria and potassium-solubilizing bacteria, inoculating the bacteria into a domestication culture medium added with wheat root exudate mother liquor, and carrying out continuous 5-8 generations of transfer culture to obtain a wheat affinity optimized flora; s2, 45-60 days before wheat sowing, relay plants are sown in the field parcels; then, the obtained wheat affinity optimization flora is prepared into an R-CRI relay microbial inoculum, and the R-CRI relay microbial inoculum is applied to a field parcel where relay plants grow; s3, within 24-48 hours before the relay plants are turned over, a relay induction signal agent containing quorum sensing signal molecules is applied to the field parcels, and then the relay plants are turned into the soil; and S4, sowing wheat in the field parcels treated in the step S3. According to the invention, by introducing a directional domestication technology based on wheat root exudates, the problem that a traditional microbial agent is not suitable for water and soil when applied in the field is solved.

This invention discloses the application of α-bisabolol in the preparation of a quorum sensing quencher for Serratia liquefaction. It belongs to the field of pharmaceutical technology. The purpose of this invention is to provide an application in the preparation of a quencher for inhibiting the quorum sensing system of Serratia liquefaction, thereby reducing the toxicity of pathogenic bacteria without inhibiting their growth. This invention provides an application of bisabolol in the preparation of a quencher for inhibiting the quorum sensing system of Serratia liquefaction. It offers a novel solution for developing efficient and safe food preservatives.

The invention relates to the technical field of agricultural resources and environment, and discloses a preparation method and application of an in-situ activator for carbon sequestration, pollution reduction and yield increase of soil. The activator comprises the following raw materials in parts by mass: 5-15 parts of a modified mineral carrier, 10-15 parts of an organic slow-release matrix, 6-13 parts of a lactic acid bacteria crude extract, 5-15 parts of a fungus crude extract, 5-12 parts of a yeast crude extract, 10-20 parts of a microbial fermentation humus crude extract, 4-8 parts of a complexing agent, 5-10 parts of a protective agent, 3-8 parts of root-promoting signal molecules and 1-3 parts of quorum sensing molecules. According to the scheme, five units including a supporting framework, an active component, a complexing agent, a protective agent and a trace substance are jointly assembled to serve as a core of the activator, the activator is supported by a porous carrier, a compound enzyme-humus system and an oxygen-iron-sulfur electron shuttle system are colonized on the surface, nutrient substance aggregates are slowly released, and signal molecule induction is combined, so that the activity of the activator is improved. And soil carbon reservoir expansion, pollutant passivation and crop root development promotion are realized. According to the preparation, the soil carbon sequestration efficiency can be improved by 25% or above, the heavy metal activity can be reduced by 40-65%, and the average yield of crops can be increased by 15-20%.