103 results about "Quorum sensing" patented technology

The invention relates to a quorum sensing intelligent regulation and control method for violaceum violaceum in an antibiotic gradient region, and aims to solve the problems of uncontrollable multi-partition microenvironment and low biological behavior consistency in an existing system, the quorum sensing intelligent regulation and control method for the violaceum violaceum in the antibiotic gradient region comprises the following steps: accurately positioning space partitions, and collecting liquid phase pH, oxygen concentration and nutrient components in each region in real time by an integrated sensor; high-throughput detection and multi-modal data fusion are combined, and an AI model is input to establish association mapping of a liquid / gas phase environment and a biological phenotype. The system automatically generates and optimizes a partition gas component adjustment instruction, realizes spatial synchronous gas phase regulation and control by utilizing a programmable micro-airflow device, and realizes multi-region environment dynamic balance regulation and control in a culture chamber.

The invention discloses a biological agent for preventing and treating panax notoginseng root rot, and relates to the technical field of biological prevention and treatment, the biological agent is composed of four layers of functional florae: an A layer of sentinel-decoding flora is used for identifying a pathogen quorum sensing signal and is used as an event trigger source, a B layer of metabolic countering flora is used for decoupling conversion of disease-promoting saponin and controllable release of a metabolic false substrate, and a C layer of functional florae is used for controlling root rot of panax notoginseng. The C-layer self-healing conductive biological membrane construction flora forms a biological membrane with conductivity and self-healing capability on the root surface, and the D-layer electrically-enabled flora constructs stable biological micro-current at the rhizosphere by using ferric oxide particles as external electron acceptors. The layer A triggers to cause controlled release of the layer B, a conversion product of the layer B provides a carbon source and a structural unit for the layer C, and a conductive network of the layer C and the layer D form an electronic path. The biological agent is used for early recognition and continuous inhibition of pathogens in panax notoginseng planting soil, can start defense response in the early stage of quorum sensing signal occurrence, and keeps action stability under complex environmental conditions.

The invention discloses a method for improving yield of branched chain aldehyde of lactococcus lactis based on regulation and control of a luxS gene and application, and belongs to the field of system biology. Gene function verification and genome scale metabolic network model analysis are creatively combined, the phenotypic effect of the luxS gene is confirmed, and more importantly, the internal action mechanism of the luxS gene is disclosed from the system level, that is, the whole metabolic network flow is influenced by regulating and controlling multiple key enzymes such as transaminase, decarboxylase, dehydrogenase and decarboxylase. The invention reveals that the quorum sensing core gene luxS has a new function of regulating and controlling synthesis of branched chain aldehyde in lactococcus lactis for the first time, and an intercellular communication system is directly linked with production of specific flavor metabolites. The invention provides a brand-new and higher-level regulation target and thought for producing flavor substances by microbial fermentation, and gets rid of the limitation that only metabolic pathway terminal enzyme is singly modified traditionally.

The application discloses a Vibrio parahaemolyticus quorum sensing inhibiting peptide and application thereof, and belongs to the field of bioactive peptides. The inhibiting peptide comprises the following amino acid sequence: Ser-Phe; the application further discloses application of the quorum sensing inhibiting peptide in inhibiting Vibrio parahaemolyticus or preparing antibacterial drugs for inhibiting Vibrio parahaemolyticus. The application adopts a molecular docking technology to screen an active peptide having an inhibiting effect on a Vibrio parahaemolyticus LuxS / AI-2 quorum sensing system, ADMET property prediction is carried out on a peptide segment with better binding force, and finally, a bioactive peptide SF stably combined with LuxP, LuxQ and LuxS is obtained; the dipeptide has good in-vitro inhibiting activity on the Vibrio parahaemolyticus LuxS / AI-2 quorum sensing, and has no cytotoxicity. The dipeptide obtained by the application can be used for guiding development of a new type of antibacterial preparation applied to food, medicine and agriculture and other fields, and has great significance for human health and agricultural development.

This invention discloses the application of δ-juniperene in the preparation of quenchers that inhibit the quorum sensing system of *Serratia liquefaction*. It belongs to the field of pharmaceutical technology. The purpose of this invention is to provide a quencher for inhibiting the quorum sensing system of *Serratia liquefaction*, thereby reducing the toxicity of pathogens without inhibiting their growth. This invention provides an application of δ-juniperene in drugs that inhibit the quorum sensing system of *Serratia liquefaction*, offering a novel solution for developing highly efficient and safe food preservatives.

Described herein is a synergistic combination comprising a quorum-sensing inhibitor and / or postbiotic metabolite and an antibiotic. Typically, the postbiotic metabolite comprises at least one peptide. Related compositions, uses, and methods are also described, including methods for resensitizing resistant bacteria to an antibiotic, and methods of treating antibiotic-resistant infections, such as methicillin-resistant Staphylococcus aureus (MRSA).

The invention discloses a piglet anti-diarrhea complete feed formula based on a microecological preparation, and relates to the technical field of animal nutrition and health. The microsphere has a unique three-layer core-shell structure, wherein a core layer is coated with butyric acid producing probiotics and precise prebiotics; the middle layer is a chitosan-gallic acid-vanillin terpolymer with dual functions of strong mucous membrane adsorbability and quorum sensing quenching; and the shell layer is a pH-sensitive slow-release coating and is loaded with an acidifying agent. The structure is constructed through a layer-by-layer coating process, and the technical problems that in the prior art, functional components cannot be delivered in a targeted mode, the synergistic effect is poor, and intestinal barriers cannot be actively repaired are solved. When the microspheres enter animal digestive tracts, the shell layer firstly releases an acidifying agent at the front end to inhibit pathogenic bacteria and clear obstacles for subsequent steps; after entering the small intestine, the middle layer polymer is released, adheres and repairs the damaged intestinal mucosa in the form of a biological bandage, and interferes pathogen communication at the same time.

The invention relates to application of extracellular vesicles in antibacterial yoga clothes, the application comprises that the extracellular vesicles are micro-fixed on the surface layer of yoga clothes fabric through a padding method or a spraying method, and the problems that chemical antibacterial agents (silver ions, graphene and the like) of existing yoga clothes are high in cost and large in skin irritation, and environment / health risks exist are solved. The extracellular vesicles are slightly fixed on the surface layer of the yoga clothes fabric through a padding method or a spraying method, and the extracellular vesicles can inhibit biological membranes formed by pathogenic bacteria such as staphylococcus aureus and escherichia coli on the surface layer of the yoga clothes fabric by interfering pathogenic bacteria quorum sensing. The fabric has the advantages that peculiar smell substances such as sulfides and fatty acid volatile substances generated by metabolism of bacteria in a sweat wet environment are reduced from the source, fabric peculiar smell control is achieved, compared with chemical antibacterial agents such as silver ions and graphene, biotoxicity risks and environmental accumulation hidden dangers are avoided, anaphylactic reactions caused by long-term contact between yoga clothes and skin can be remarkably reduced, and wearing safety is improved.

This invention belongs to the fields of genetic engineering and microbial technology, specifically relating to a yeast quorum sensing dynamic regulation system, method, and its applications. This invention successfully constructs a functional, bacterial-derived RpaI / RpaR quorum sensing system in yeast for the first time. The constructed yeast quorum sensing dynamic regulation system possesses advantages such as low background leakage, high sensitivity, and wide dynamic range. By flexibly coupling the core system with a signal amplification module (Gal4) and a signal conversion module (CRISPRi), a bifunctional cascade platform integrating activation and inhibition is constructed, achieving multidimensional and precise dynamic regulation of metabolic pathways. Furthermore, the system has been applied to the biosynthesis of several important compounds, including cordycepin, geraniol, and 3-hydroxypropionic acid, significantly increasing yields and effectively balancing the conflict between cell growth and product synthesis, demonstrating its great potential as a universal dynamic regulation tool.

The invention relates to the technical field of biotechnology, enzyme engineering and biological pharmacy, in particular to a ribonuclease purification production method. According to the method, a deep sea antifreeze polypeptide and rare earth nanocrystal composite bionic system is constructed, quorum sensing signal molecules and a pulsed electric field are introduced in a fermentation stage for two-factor regulation, and a light-operated micro-fluidic chip selective extraction technology is combined, so that efficient separation of target enzyme is realized. A double-ligand magnetic microsphere chromatographic column is innovatively adopted for gradient elution, and the enzyme activity retention rate and the long-term storage stability are remarkably improved through thermotropic liquid crystal capsule stabilization and radio frequency assisted freeze drying processes. According to the invention, the destructiveness of the traditional chromatography technology to enzyme conformation is broken through, the problem of batch fluctuation caused by multi-step purification is solved, a ribonuclease product with high purity, high activity and environmental response characteristic is provided for the field of biological medicine, and the ribonuclease is especially suitable for industrial preparation of gene therapy products and virus vectors.

The invention relates to the technical field of agricultural biology, and discloses a high-yield wheat planting method which comprises the following steps: S1, screening and compounding nitrogen-fixing bacteria, phosphate-solubilizing bacteria and potassium-solubilizing bacteria, inoculating the bacteria into a domestication culture medium added with wheat root exudate mother liquor, and carrying out continuous 5-8 generations of transfer culture to obtain a wheat affinity optimized flora; s2, 45-60 days before wheat sowing, relay plants are sown in the field parcels; then, the obtained wheat affinity optimization flora is prepared into an R-CRI relay microbial inoculum, and the R-CRI relay microbial inoculum is applied to a field parcel where relay plants grow; s3, within 24-48 hours before the relay plants are turned over, a relay induction signal agent containing quorum sensing signal molecules is applied to the field parcels, and then the relay plants are turned into the soil; and S4, sowing wheat in the field parcels treated in the step S3. According to the invention, by introducing a directional domestication technology based on wheat root exudates, the problem that a traditional microbial agent is not suitable for water and soil when applied in the field is solved.

This invention discloses the application of α-bisabolol in the preparation of a quorum sensing quencher for Serratia liquefaction. It belongs to the field of pharmaceutical technology. The purpose of this invention is to provide an application in the preparation of a quencher for inhibiting the quorum sensing system of Serratia liquefaction, thereby reducing the toxicity of pathogenic bacteria without inhibiting their growth. This invention provides an application of bisabolol in the preparation of a quencher for inhibiting the quorum sensing system of Serratia liquefaction. It offers a novel solution for developing efficient and safe food preservatives.

The invention relates to the technical field of agricultural resources and environment, and discloses a preparation method and application of an in-situ activator for carbon sequestration, pollution reduction and yield increase of soil. The activator comprises the following raw materials in parts by mass: 5-15 parts of a modified mineral carrier, 10-15 parts of an organic slow-release matrix, 6-13 parts of a lactic acid bacteria crude extract, 5-15 parts of a fungus crude extract, 5-12 parts of a yeast crude extract, 10-20 parts of a microbial fermentation humus crude extract, 4-8 parts of a complexing agent, 5-10 parts of a protective agent, 3-8 parts of root-promoting signal molecules and 1-3 parts of quorum sensing molecules. According to the scheme, five units including a supporting framework, an active component, a complexing agent, a protective agent and a trace substance are jointly assembled to serve as a core of the activator, the activator is supported by a porous carrier, a compound enzyme-humus system and an oxygen-iron-sulfur electron shuttle system are colonized on the surface, nutrient substance aggregates are slowly released, and signal molecule induction is combined, so that the activity of the activator is improved. And soil carbon reservoir expansion, pollutant passivation and crop root development promotion are realized. According to the preparation, the soil carbon sequestration efficiency can be improved by 25% or above, the heavy metal activity can be reduced by 40-65%, and the average yield of crops can be increased by 15-20%.

The invention discloses a multistage enzyme and quorum sensing signal molecule MABR membrane and a preparation method thereof, through a bionic induction strategy, while the anti-pollution characteristic of the MABR membrane is maintained, multistage enzyme and quorum sensing signal molecules are fixed on the surface of the membrane, and the multistage enzyme and quorum sensing signal molecules induce microorganisms to secrete an extracellular polymeric substance (EPS), so that the binding strength of a biological membrane is improved by 40% or more. The preparation method of the multi-stage enzyme and quorum sensing signal molecule MABR membrane comprises the following steps: (1) screening nitrifying bacteria and extracting multi-stage enzyme and quorum sensing signal molecules of the nitrifying bacteria; (2) carrying out composite pretreatment on the surface of the MABR membrane, wherein the composite pretreatment comprises hydrophilic modification, functional group introduction, swelling of the surface of membrane silk and the like; and (3) immobilizing multistage enzyme and quorum sensing signal molecules (including cell membrane fragments, periplasmic space enzyme complexes and the like) in the strain by adopting an immobilization technology to form a bioactive interface layer with the thickness of about 50-200nm.

The invention discloses a green fluorescence report system for screening a Listeria monocytogenes quorum sensing inhibitor as well as a construction method and application of the green fluorescence report system. The report system is constructed through the following steps: synthesizing a DNA fragment according to a listeria monocytogenes AGR system pII promoter sequence, connecting the DNA fragment with a green fluorescent protein pEGFP plasmid, amplifying an EGFP-pII target fragment through PCR, and connecting the target fragment to a pKSV7 shuttle vector plasmid through homologous recombination to obtain a pKSV7-EGFP-pII plasmid, and then transferring the gene into a competent cell of listeria monocytogenes ATCC BAA-679, and screening to obtain a report system. The report system disclosed by the invention can be used for intuitively evaluating the expression condition of the quorum sensing system of the listeria monocytogenes and screening out the quorum sensing inhibitor.

The invention discloses a cinnamyl aldehyde modified montmorillonite nanosheet capable of inhibiting bacterial quorum sensing as well as a preparation method and application of the cinnamyl aldehyde modified montmorillonite nanosheet. According to the modified nanosheet, a two-dimensional montmorillonite nanosheet is taken as a carrier, and cinnamyl aldehyde is intercalated into an interlayer region of the two-dimensional montmorillonite nanosheet. The modified montmorillonite nanosheet can achieve the effect of inhibiting inflammation by inhibiting bacterial quorum sensing and adjusting the abundance of intestinal microorganisms, effectively relieves symptoms induced by IBD, has good stability, biocompatibility and biodegradability, and can be used for treating inflammatory bowel diseases.

The invention discloses a microbial agent for treating agriculture, forestry, livestock and poultry wastes and a preparation method thereof, the microbial agent is composed of salt-tolerant bacillus, acid-resistant nitrococcus, bacillus amyloliquefaciens and pantoea agglomerans according to a weight part ratio of 3: 2: 1: 0.5, and the microbial agent is subjected to gradient stress-AHLs signal induction domestication. The preparation method comprises the steps of seed culture, synergistic induction culture and microcapsule embedding, active flora synergy is achieved through quorum sensing, and the three-in-one function of degradation, growth promotion and disease resistance is achieved. The microbial agent is high in stress resistance, the shelf life is 12-18 months, the degradation rate of cellulose in 7 days is 65%-75%, the microbial agent is suitable for resource utilization of various agricultural, forestry, livestock and poultry wastes, and a treated product can be directly used as a functional organic fertilizer.

The invention discloses a catechin silver nano antibacterial particle as well as a preparation method and application thereof, and belongs to the technical field of antibacterial materials. The silver catechin nano antibacterial particles disclosed by the invention are Ct-Ag NPs, and the silver catechin nano antibacterial particles are Ct-Ag NPs; the Ct-Ag NPs is of a spherical structure; the average particle size of the Ct-Ag NPs ranges from 142 nm to 162 nm; ag in the Ct-Ag NPs is uniformly dispersed in catechin to form a core-shell structure. The preparation method of the silver catechin nano antibacterial particles comprises the following steps: weighing catechin, and dissolving the catechin in a sodium hydroxide solution to obtain a catechin sodium salt solution; adding the silver nitrate solution into the ammonia water solution to obtain a silver ammonia solution; and under the conditions of stirring and ice bath, adding the catechin sodium salt solution into the silver ammonia solution, centrifuging, cleaning with trichloromethane, and drying to obtain the Ct-Ag NPs. The Ct-Ag NPs provided by the invention has a core-shell structure, and the quorum sensing inhibitor shell Ct can effectively reduce the generation of biological membranes, so that bacteria are more sensitive, and the bactericidal effect of continuously releasing Ag < + > is effectively improved.

The invention relates to the field of polypeptides, in particular to an NL-1 polypeptide capable of inhibiting a tight reaction and application of the NL-1 polypeptide. The NL-1 polypeptide is screened from transcripts of spiders, and the NL-1 polypeptide is predicted and verified to have antibacterial activity through antibacterial screening. An enzyme activity experiment is utilized to prove that synthesis of a tight reaction signal small molecule ppGpp can be reduced by inhibiting activity of key enzymes PaRelA and PaSpoT in a tight reaction of pseudomonas aeruginosa, so that the tight reaction is inhibited. Meanwhile, a bacteriostatic experiment and a bactericidal dynamics experiment prove that the NL-1 polypeptide grows in a concentration-dependent manner through three hospital-sourced multidrug-resistant pseudomonas aeruginosa strain; meanwhile, the NL-1 can effectively kill multidrug-resistant pseudomonas aeruginosa, in addition, the NL-1 also effectively reduces the generation of a quorum sensing key molecule PQS of the pseudomonas aeruginosa, and the component is proved to be related to antibiotic tolerance. In conclusion, the NL-1 polypeptide disclosed by the invention has a good antibacterial effect, can be used as a novel antibiotic, and has a huge application value for treatment of multidrug-resistant bacteria.

The present application relates to the low-temperature food preservative and fresh-keeping technical field, and particularly provides a quorum sensing inhibitor and its application in sturgeon preservative and fresh-keeping, specifically, the fresh-keeping method of vacuum packaging sturgeon provided by the present application comprises the following steps: placing the sturgeon into a resveratrol solution with a concentration of 0.1 mM-0.5 mM at 0-4 DEG C, taking out and draining, and vacuum packaging.The present application utilizes the quorum sensing inhibition effect of resveratrol to provide an efficient, green and safe preservative and fresh-keeping technology for low-temperature storage food, especially sturgeon, without adding chemical preservatives.

This invention discloses the application of 6-methyladenine in the preparation of drugs against Pseudomonas aeruginosa infection. This invention provides the application of 6-methyladenine in the preparation of drugs against Pseudomonas aeruginosa (… Pseudomonas aeruginosa Application in infectious agents. The 6-methyladenine described in this invention inhibits the quorum sensing of Pseudomonas aeruginosa and the production of related virulence factors, thereby suppressing Pseudomonas aeruginosa infection in vivo and in vitro, reducing host mortality, and providing a new therapeutic strategy for the prevention and control of Pseudomonas aeruginosa infection.

The application discloses a kind of recombination of Pseudomonas putida for producing L-lactic acid using whole-component lignocellulose hydrolysate and its construction method and application, belong to the field of metabolic engineering.The application uses Pseudomonas putida KT2440 as chassis, realizes carbon flow to pyruvic acid convergence and prevents product degradation by knocking out aceEF 、 ppsA And lldD Gene;By in-situ replacement, introduce optimized Lactobacillus plantarum ldhL1 Reconfiguration L-lactic acid synthesis pathway;By integration xylD-yjhH Tandem expression cassette enables xylose utilization;And by quorum sensing promoter P Rox306 Overexpression maeB Realize funnel conversion of lignin monomer.The application innovatively uses lignin derivatives in hydrolysate as natural donor of endogenous acetyl-coenzyme A, makes up lethal growth defect caused by knocking out aceEF The engineering bacteria of the application realize the synchronous high-efficiency conversion of glucose, xylose and aromatic monomer under the condition of not adding exogenous auxiliary carbon source and chemical inducer, which greatly reduces the industrial fermentation cost.

A catheter clip-on device applies surface acoustic waves to a catheter for preventing biofilms on the catheter surfaces. Multiple frequencies are generated by at least two PZT elements, coupled to a catheter or in-dwelling device. The relative motion of bacteria results in bacteria quorum sensing, and disrupts the bacteria attachment process.

Described herein are recombinant, attenuated bacteria with an altered quorum sensing pathway. The recombinant bacteria are used for visualizing quorum sensing or immunizing marine organisms against infections by Vibrio spp.

The invention discloses an intestinal flora regulating composition for targeting drug-resistant enterobacter and application thereof, and relates to the technical field of biological medicines and microecological preparations, in particular to an intestinal flora regulating composition for targeting drug-resistant enterobacter and application thereof, the active ingredients comprise bacillus capable of interfering a drug-resistant enterobacter quorum sensing system, bacillus capable of interfering a drug-resistant enterobacter quorum sensing system, bacillus capable of interfering a drug-resistant enterobacter quorum sensing system, and bacillus capable of interfering the drug-resistant enterobacter quorum sensing system. The bacillus subtilis can be bacillus coagulans or bacillus subtilis; saccharomycetes capable of adsorbing the drug-resistant enterobacter and consuming necessary trace elements thereof, such as saccharomyces cerevisiae with optimized cell walls; the oligosaccharide mixture can be utilized by beneficial bacteria and can generate inhibitory products after being subjected to specific enzymolysis by drug-resistant enterobacter, and galactooligosaccharide, xylooligosaccharide and seaweed-derived oligosaccharide are mixed according to a specific proportion. The composition can be used for reducing the loading capacity of drug-resistant enterobacter in intestinal tracts of mammals, restoring the balance of intestinal flora and reducing the systemic infection risk. The preparation method comprises the step of mixing the active ingredients with a pharmaceutically or food acceptable carrier to prepare an oral dosage form.

The invention relates to a method for realizing two-way control of the particle size of aerobic granular sludge by coupling hydraulic selection and floc addition, which comprises the following steps: in the aerobic granular sludge culture process, forming hydraulic selection pressure by gradually shortening the precipitation time to promote sludge granulation, and monitoring the particle size change condition of a system in the granulation process; when the particle size of the particles is rapidly increased, taking floc sludge as additional sludge, regularly adding the floc sludge into the system according to a certain proportion, slowing down the granulation speed of the sludge, strengthening the diversity and mass transfer capacity of microorganisms in the system, and monitoring the particle size change condition during the floc adding period; analyzing a regulation and control mechanism of hydraulic selection and floc addition on microbial population sensing behaviors in the granulation process; the particle size of the aerobic granular sludge is subjected to two-way regulation and control by hydraulic selection and floc addition, so that the granulation process is maintained in a reasonable interval, the sacrifice of microbial diversity and particle mass transfer capacity in the granulation process is made up, and the system is more stable in microbial cooperation.

The invention discloses application of morin, and relates to the technical field of bacterial quorum sensing inhibition. The invention discloses application of morin in preparation of a medicine for reducing enterotoxigenic escherichia coli quorum sensing. Quorum sensing of enterotoxigenic escherichia coli can be reduced by using 5.0 mg / mL of Morrin, so that secretion of virulence factors of the enterotoxigenic escherichia coli is reduced. In the Escherichia coli treated by the Morin, the Morin has no obvious inhibition effect on the growth of the Escherichia coli, and the abundance of an autoinducer 2 (AI-2) and a quorum sensing related gene is obviously reduced, so that the toxicity and the adhesion capability of the Escherichia coli are reduced. The method for reducing enterotoxigenic Escherichia coli quorum sensing provided by the invention is beneficial to replacing traditional antibiotic in-vivo treatment and assists in resisting bacterial infection.

The invention provides a mild membrane pollution cleaning method based on group sensing inhibition and habitat regulation and control. The method comprises the following steps: starting a cleaning process when operating parameters of a membrane system deviate from a set operating range, and carrying out group sensing inhibition mild cleaning on a membrane; in the cleaning process, low-strength acid-base pre-cleaning is selectively introduced according to the running state of the membrane system; after cleaning is completed, the cleaning state is judged according to the membrane system operation parameters till the membrane system operation parameters are recovered to the set operation range. In the cleaning process, microbial quorum sensing signal transmission in the membrane pollution layer is interfered through a quorum sensing inhibition effect, the effect of extracellular polymeric substances on the structural stability of the pollution layer is weakened, and the living conditions of microorganisms in the membrane pollution layer are changed through habitat regulation and control; therefore, loosening and stripping of a membrane pollution layer formed by biological pollution, organic pollution, inorganic pollution or a combination thereof are realized under a non-caustic condition. The method avoids the use of a strong oxidizing bactericide, and is suitable for the pollution control and cleaning process of different types of membrane systems.

The invention relates to the field of medicines, and discloses catechol coupled bibenzothiazole compounds shown in a formula I and application of the catechol coupled bibenzothiazole compounds in formation of antibacterial biofilms. In-vivo and in-vitro pharmacodynamic verification shows that most of the catechol coupled bibenzothiazole compounds have excellent bacterial biofilm, quorum sensing and bacterial iron uptake inhibitory activity, can be used for preparing broad-spectrum antibiotic synergists, and can enhance the antibacterial effect of existing antibiotics. Formula I

The application discloses application of barley malt alkali in inhibition of growth and pathogenicity of Bacillus cereus by regulating a quorum sensing system of the Bacillus cereus. The barley malt alkali is a green natural product, is safer for the environment and human bodies than traditional chemical antibacterial agents such as antibiotics, and reduces the risk of drug residue and drug-resistant strain generation. Research results show that the barley malt alkali can play a role at a low concentration (1 / 8 MIC), so that the use cost is low. The application can target regulation of expression of multiple key genes (such as Spo0A, CodY, rpoN and the like), so as to not only inhibit pathogenicity, but also effectively control proliferation of the Bacillus cereus, reduce the risk of food contamination and foodborne disease infection, and has important application prospects in the field of food safety.