165 results about "Quorum sensing" patented technology

The invention belongs to the field of microbial preparations, and particularly relates to a complex microbial inoculant for promoting microbial quorum sensing and application of the complex microbial inoculant in compost. The composite microbial agent comprises pseudomonas A3 and paenibacillus polymyxa 21-2, and the preservation number of the pseudomonas A3 is CCTCC (China Center for Type Culture Collection) NO: M20251394. The pseudomonas A3 can express key genes such as rhlI and lasI of a gram-negative bacteria AHL quorum sensing system and signal molecules of the key genes, and the paenibacillus polymyxa 21-2 can express luxS genes of a gram-positive bacteria AI-2 signal system and signal molecules DPD of the luxS genes. In the pig manure compost, the complex microbial inoculant is added, so that the expression of AI-2 and AHLs system related genes in the compost and the content of signal molecules DPD are remarkably improved, the microbial population sensing process of the compost is promoted, finally, the compost temperature is increased by about 6 DEG C, the compost carbon-nitrogen ratio is reduced, and the compost maturity is promoted.

The invention relates to a quorum sensing intelligent regulation and control method for violaceum violaceum in an antibiotic gradient region, and aims to solve the problems of uncontrollable multi-partition microenvironment and low biological behavior consistency in an existing system, the quorum sensing intelligent regulation and control method for the violaceum violaceum in the antibiotic gradient region comprises the following steps: accurately positioning space partitions, and collecting liquid phase pH, oxygen concentration and nutrient components in each region in real time by an integrated sensor; high-throughput detection and multi-modal data fusion are combined, and an AI model is input to establish association mapping of a liquid / gas phase environment and a biological phenotype. The system automatically generates and optimizes a partition gas component adjustment instruction, realizes spatial synchronous gas phase regulation and control by utilizing a programmable micro-airflow device, and realizes multi-region environment dynamic balance regulation and control in a culture chamber.

The invention provides a dynamic control system for coordinating streptomyces secondary metabolite production and application of the dynamic control system. Firstly, a general artificial promoter PQS (trigger) responding to a quorum sensing system of various streptomycetes is designed, then a stabilizer is constructed based on a bistable gene circuit, instantaneous signal output is converted into stable signal output, dependence of an inducer is successfully eliminated, finally, an orthogonal amplifier is developed based on a streptomycete rare extracellular function sigma factor, and finally, the specific function of the quorum sensing system of various streptomycetes is achieved. And different promoter element libraries containing amplifiers with response from weak to strong and with strength span of about 535 times are constructed. An amplifier and a CRISPRi system are combined to construct a turner, and gene expression inhibition with the strength span of about 200 times is achieved. The combination of the amplifier, the promoter library and the turner is called a multi-effect device, and abundant elements are provided for fine target gene expression. The combination of the trigger, the stabilizer and the multiple-effect device is called as a streptomycete multipath manual control system (SMARTS), and control tools of streptomycete are enriched.

The invention relates to the field of rice seedling raising cultivation, and discloses a preparation method of a pest-resistant orderly-released rice seedling raising substrate, which comprises the following steps: (a) preparing a composite microcapsule which comprises a functional microorganism core and an intelligent response type shell coating the functional microorganism core; wherein a compound microbial flora and a quorum sensing signal precursor are loaded in the functionalized microbial core; the intelligent response type shell contains a plant immunity inducer, and the material of the shell is set to be degraded under the action of rice root exudates. By designing a composite microcapsule structure, programmed and targeted release of active substances is realized, so that a plant immunity inducer acts in advance in the early stage of seedling raising, and a composite microbial flora is accurately released to a rhizosphere area only when a rice root system is in contact with the microcapsule, so that the effect of raising seedlings is achieved. And the utilization efficiency and the action accuracy of each functional component are obviously improved.

The invention relates to the technical field of soil fertility management, in particular to a production quality control method of a special fertilizer for Yunnan cigar tobacco leaves, which comprises the following steps: step 1, preparing a waste-based functional carrier; step 2, signal response type fermentation control: based on a dynamic monitoring result of microbial population sensing signal molecules in a fermentation system, regulating and controlling the injection opportunity of an exogenous inducer and fermentation environment parameters, and directionally synthesizing a targeted antibacterial metabolite; step 3, adaptive packaging in a growing period; and 4, bionic verification and closed-loop optimization: verifying a nutrient release curve through a bionic root system simulation device, and feeding back and adjusting packaging parameters in combination with a field metabolite detection result to form a production-application self-optimization system. The production efficiency and fertilizer efficiency of the cigar tobacco leaf fertilizer are improved through accurate process control, it is ensured that nutrient supply and release of the fertilizer in all growth periods have excellent matching performance, and the effect of the fertilizer can be optimized in practical application.

The invention relates to a method for controlling biological pollution of a reverse osmosis membrane through photoelectrochemical filtration pretreatment. According to the method, a photoelectrochemical reactor is mainly established to inhibit the bacterial biofilm forming ability under ultraviolet illumination and voltage conditions. The photoelectric chemical reactor is composed of a peristaltic pump, a magnetic stirrer, a reactor, an electrochemical filtering assembly, a direct-current power source and an ultraviolet light source and inhibits bacteria added into the reactor from forming a biological membrane. Under the action of voltage, the electrochemical filter assembly inhibits the biofilm forming ability and viability of bacteria by degrading protein and quorum sensing signal molecules in water, and more thoroughly inhibits the biofilm formation through the irradiation of ultraviolet light. Therefore, according to the method disclosed by the invention, the ultraviolet light can be combined with the electrochemical filter assembly to realize effective inhibition of a biological membrane formed by bacteria, the reduction of membrane flux is reduced, and the method has important significance in controlling biological pollution of the reverse osmosis membrane.

The invention discloses a biological agent for preventing and treating panax notoginseng root rot, and relates to the technical field of biological prevention and treatment, the biological agent is composed of four layers of functional florae: an A layer of sentinel-decoding flora is used for identifying a pathogen quorum sensing signal and is used as an event trigger source, a B layer of metabolic countering flora is used for decoupling conversion of disease-promoting saponin and controllable release of a metabolic false substrate, and a C layer of functional florae is used for controlling root rot of panax notoginseng. The C-layer self-healing conductive biological membrane construction flora forms a biological membrane with conductivity and self-healing capability on the root surface, and the D-layer electrically-enabled flora constructs stable biological micro-current at the rhizosphere by using ferric oxide particles as external electron acceptors. The layer A triggers to cause controlled release of the layer B, a conversion product of the layer B provides a carbon source and a structural unit for the layer C, and a conductive network of the layer C and the layer D form an electronic path. The biological agent is used for early recognition and continuous inhibition of pathogens in panax notoginseng planting soil, can start defense response in the early stage of quorum sensing signal occurrence, and keeps action stability under complex environmental conditions.

The invention discloses a method for improving yield of branched chain aldehyde of lactococcus lactis based on regulation and control of a luxS gene and application, and belongs to the field of system biology. Gene function verification and genome scale metabolic network model analysis are creatively combined, the phenotypic effect of the luxS gene is confirmed, and more importantly, the internal action mechanism of the luxS gene is disclosed from the system level, that is, the whole metabolic network flow is influenced by regulating and controlling multiple key enzymes such as transaminase, decarboxylase, dehydrogenase and decarboxylase. The invention reveals that the quorum sensing core gene luxS has a new function of regulating and controlling synthesis of branched chain aldehyde in lactococcus lactis for the first time, and an intercellular communication system is directly linked with production of specific flavor metabolites. The invention provides a brand-new and higher-level regulation target and thought for producing flavor substances by microbial fermentation, and gets rid of the limitation that only metabolic pathway terminal enzyme is singly modified traditionally.

The invention belongs to the technical field of biological medicine, and particularly relates to application of 4-imidazole formaldehyde in a pseudomonas aeruginosa quorum sensing inhibitor. It is found for the first time that 4-imidazole formaldehyde can inhibit quorum sensing of pseudomonas aeruginosa, reduce QS system regulation gene expression and virulence factor yield and remarkably reduce infection caused by QS system regulation gene expression and virulence factor yield; the drug application designed on the basis of bacterial metabolism can avoid the drug resistance of a conventional bacteriostatic agent, and a new strategy is provided for prevention and treatment of pseudomonas aeruginosa infection.

The invention discloses an application of bilobalide in preparation of a product for resisting salmonella typhimurium infection. The bilobalide takes salmonella typhimurium QseB protein as a target, has a good interference effect on an AI-2 quorum sensing signal of salmonella typhimurium and has good pharmacological activity, and particularly, the bilobalide has good pharmacological activity under the condition that the growth of salmonella typhimurium is not inhibited. The motility, biofilm formation and infection pathogenicity of salmonella typhimurium are obviously inhibited. The bilobalide does not influence the growth of salmonella typhimurium, directly acts on pathogenic factors, weakens the pathogenicity of bacteria from the source, does not generate selective pressure on salmonella typhimurium, and does not cause the generation of drug-resistant pathogenic bacteria. Therefore, the bilobalide has a good application prospect in the aspect of development of the salmonella typhimurium infection resisting medicine.

The application discloses a Vibrio parahaemolyticus quorum sensing inhibiting peptide and application thereof, and belongs to the field of bioactive peptides. The inhibiting peptide comprises the following amino acid sequence: Ser-Phe; the application further discloses application of the quorum sensing inhibiting peptide in inhibiting Vibrio parahaemolyticus or preparing antibacterial drugs for inhibiting Vibrio parahaemolyticus. The application adopts a molecular docking technology to screen an active peptide having an inhibiting effect on a Vibrio parahaemolyticus LuxS / AI-2 quorum sensing system, ADMET property prediction is carried out on a peptide segment with better binding force, and finally, a bioactive peptide SF stably combined with LuxP, LuxQ and LuxS is obtained; the dipeptide has good in-vitro inhibiting activity on the Vibrio parahaemolyticus LuxS / AI-2 quorum sensing, and has no cytotoxicity. The dipeptide obtained by the application can be used for guiding development of a new type of antibacterial preparation applied to food, medicine and agriculture and other fields, and has great significance for human health and agricultural development.

This invention discloses the application of δ-juniperene in the preparation of quenchers that inhibit the quorum sensing system of *Serratia liquefaction*. It belongs to the field of pharmaceutical technology. The purpose of this invention is to provide a quencher for inhibiting the quorum sensing system of *Serratia liquefaction*, thereby reducing the toxicity of pathogens without inhibiting their growth. This invention provides an application of δ-juniperene in drugs that inhibit the quorum sensing system of *Serratia liquefaction*, offering a novel solution for developing highly efficient and safe food preservatives.

Described herein is a synergistic combination comprising a quorum-sensing inhibitor and / or postbiotic metabolite and an antibiotic. Typically, the postbiotic metabolite comprises at least one peptide. Related compositions, uses, and methods are also described, including methods for resensitizing resistant bacteria to an antibiotic, and methods of treating antibiotic-resistant infections, such as methicillin-resistant Staphylococcus aureus (MRSA).

Provided is an engineered bacterium for inhibiting the synthesis of dodecanedioic acid. The expression of a related redox gene is knocked out or down-regulated in the genome of the engineered bacterium. The engineered bacterium can effectively reduce the production of the by-product dodecanedioic acid and prevent the peroxidation of 12-hydroxydodecanoic acid during the synthesis of nylon 12 monomer. Further provided is a method for constructing an engineered bacterium for inhibiting the synthesis of dodecanedioic acid. The method involves synthesizing nylon 12 monomer in Escherichia coli by using a quorum-sensing system in Vibrio fischeri. The established Escherichia coli containing a self-induction system can produce the nylon 12 monomer from glucose, and the generation of the by-product dodecanedioic acid can be effectively reduced. After fermentation in a shake flask for 72 h, the yield of the nylon 12 monomer is more than 100 mg / L, and the yield of the by-product dodecanedioic acid is reduced to less than 200 mg / L (the proportion in the product is less than 20%).

The invention discloses a piglet anti-diarrhea complete feed formula based on a microecological preparation, and relates to the technical field of animal nutrition and health. The microsphere has a unique three-layer core-shell structure, wherein a core layer is coated with butyric acid producing probiotics and precise prebiotics; the middle layer is a chitosan-gallic acid-vanillin terpolymer with dual functions of strong mucous membrane adsorbability and quorum sensing quenching; and the shell layer is a pH-sensitive slow-release coating and is loaded with an acidifying agent. The structure is constructed through a layer-by-layer coating process, and the technical problems that in the prior art, functional components cannot be delivered in a targeted mode, the synergistic effect is poor, and intestinal barriers cannot be actively repaired are solved. When the microspheres enter animal digestive tracts, the shell layer firstly releases an acidifying agent at the front end to inhibit pathogenic bacteria and clear obstacles for subsequent steps; after entering the small intestine, the middle layer polymer is released, adheres and repairs the damaged intestinal mucosa in the form of a biological bandage, and interferes pathogen communication at the same time.

The present invention relates to the preparation and use of high-entropy nanozymes with multi-pathway synergistic antibacterial effects, belonging to the field of nanozyme catalytic materials. The present invention, for the first time, utilizes a hydrothermal method and high-temperature calcination method to synthesize oxygen-vacancy-rich high-entropy oxides with haloperoxidase-like, peroxidase-like, and glutathione oxidase-like activities. These oxides can catalyze bromide ions and hydrogen peroxide to generate hypobromous acid, which interferes with bacterial quorum sensing, and hydroxyl radicals, which disrupt structure and function. Furthermore, these oxides consume glutathione, which maintains the internal redox balance of bacteria. This allows for multi-pathway synergistic and efficient bacterial damage and inactivation, helping to address the problems of existing antifouling nanozyme materials, which suffer from a single catalytic pathway and poor component adjustability. This allows for the practical expansion of high-entropy nanozyme coatings, and has potential application prospects in the field of marine biofouling.

The invention relates to application of extracellular vesicles in antibacterial yoga clothes, the application comprises that the extracellular vesicles are micro-fixed on the surface layer of yoga clothes fabric through a padding method or a spraying method, and the problems that chemical antibacterial agents (silver ions, graphene and the like) of existing yoga clothes are high in cost and large in skin irritation, and environment / health risks exist are solved. The extracellular vesicles are slightly fixed on the surface layer of the yoga clothes fabric through a padding method or a spraying method, and the extracellular vesicles can inhibit biological membranes formed by pathogenic bacteria such as staphylococcus aureus and escherichia coli on the surface layer of the yoga clothes fabric by interfering pathogenic bacteria quorum sensing. The fabric has the advantages that peculiar smell substances such as sulfides and fatty acid volatile substances generated by metabolism of bacteria in a sweat wet environment are reduced from the source, fabric peculiar smell control is achieved, compared with chemical antibacterial agents such as silver ions and graphene, biotoxicity risks and environmental accumulation hidden dangers are avoided, anaphylactic reactions caused by long-term contact between yoga clothes and skin can be remarkably reduced, and wearing safety is improved.

The present invention belongs to the field of pharmaceutical technology and relates to a preparation that binds to the AbaR protein of Acinetobacter baumannii and its application. The present invention utilizes sansinol O to effectively bind to the AbaR protein in Acinetobacter baumannii, thereby inhibiting its AHL quorum sensing system. It has been confirmed that sansinol O can significantly inhibit the biofilm formation ability of Acinetobacter baumannii ATCC 19606 strain, reduce bacterial motility, and reduce bacterial toxicity to cells, without significantly affecting bacterial growth and causing selective pressure to promote bacterial evolution of drug resistance.

This invention belongs to the fields of genetic engineering and microbial technology, specifically relating to a yeast quorum sensing dynamic regulation system, method, and its applications. This invention successfully constructs a functional, bacterial-derived RpaI / RpaR quorum sensing system in yeast for the first time. The constructed yeast quorum sensing dynamic regulation system possesses advantages such as low background leakage, high sensitivity, and wide dynamic range. By flexibly coupling the core system with a signal amplification module (Gal4) and a signal conversion module (CRISPRi), a bifunctional cascade platform integrating activation and inhibition is constructed, achieving multidimensional and precise dynamic regulation of metabolic pathways. Furthermore, the system has been applied to the biosynthesis of several important compounds, including cordycepin, geraniol, and 3-hydroxypropionic acid, significantly increasing yields and effectively balancing the conflict between cell growth and product synthesis, demonstrating its great potential as a universal dynamic regulation tool.

The invention relates to the technical field of biotechnology, enzyme engineering and biological pharmacy, in particular to a ribonuclease purification production method. According to the method, a deep sea antifreeze polypeptide and rare earth nanocrystal composite bionic system is constructed, quorum sensing signal molecules and a pulsed electric field are introduced in a fermentation stage for two-factor regulation, and a light-operated micro-fluidic chip selective extraction technology is combined, so that efficient separation of target enzyme is realized. A double-ligand magnetic microsphere chromatographic column is innovatively adopted for gradient elution, and the enzyme activity retention rate and the long-term storage stability are remarkably improved through thermotropic liquid crystal capsule stabilization and radio frequency assisted freeze drying processes. According to the invention, the destructiveness of the traditional chromatography technology to enzyme conformation is broken through, the problem of batch fluctuation caused by multi-step purification is solved, a ribonuclease product with high purity, high activity and environmental response characteristic is provided for the field of biological medicine, and the ribonuclease is especially suitable for industrial preparation of gene therapy products and virus vectors.

The invention relates to the technical field of agricultural biology, and discloses a high-yield wheat planting method which comprises the following steps: S1, screening and compounding nitrogen-fixing bacteria, phosphate-solubilizing bacteria and potassium-solubilizing bacteria, inoculating the bacteria into a domestication culture medium added with wheat root exudate mother liquor, and carrying out continuous 5-8 generations of transfer culture to obtain a wheat affinity optimized flora; s2, 45-60 days before wheat sowing, relay plants are sown in the field parcels; then, the obtained wheat affinity optimization flora is prepared into an R-CRI relay microbial inoculum, and the R-CRI relay microbial inoculum is applied to a field parcel where relay plants grow; s3, within 24-48 hours before the relay plants are turned over, a relay induction signal agent containing quorum sensing signal molecules is applied to the field parcels, and then the relay plants are turned into the soil; and S4, sowing wheat in the field parcels treated in the step S3. According to the invention, by introducing a directional domestication technology based on wheat root exudates, the problem that a traditional microbial agent is not suitable for water and soil when applied in the field is solved.

This invention discloses the application of α-bisabolol in the preparation of a quorum sensing quencher for Serratia liquefaction. It belongs to the field of pharmaceutical technology. The purpose of this invention is to provide an application in the preparation of a quencher for inhibiting the quorum sensing system of Serratia liquefaction, thereby reducing the toxicity of pathogenic bacteria without inhibiting their growth. This invention provides an application of bisabolol in the preparation of a quencher for inhibiting the quorum sensing system of Serratia liquefaction. It offers a novel solution for developing efficient and safe food preservatives.

The invention relates to the technical field of agricultural resources and environment, and discloses a preparation method and application of an in-situ activator for carbon sequestration, pollution reduction and yield increase of soil. The activator comprises the following raw materials in parts by mass: 5-15 parts of a modified mineral carrier, 10-15 parts of an organic slow-release matrix, 6-13 parts of a lactic acid bacteria crude extract, 5-15 parts of a fungus crude extract, 5-12 parts of a yeast crude extract, 10-20 parts of a microbial fermentation humus crude extract, 4-8 parts of a complexing agent, 5-10 parts of a protective agent, 3-8 parts of root-promoting signal molecules and 1-3 parts of quorum sensing molecules. According to the scheme, five units including a supporting framework, an active component, a complexing agent, a protective agent and a trace substance are jointly assembled to serve as a core of the activator, the activator is supported by a porous carrier, a compound enzyme-humus system and an oxygen-iron-sulfur electron shuttle system are colonized on the surface, nutrient substance aggregates are slowly released, and signal molecule induction is combined, so that the activity of the activator is improved. And soil carbon reservoir expansion, pollutant passivation and crop root development promotion are realized. According to the preparation, the soil carbon sequestration efficiency can be improved by 25% or above, the heavy metal activity can be reduced by 40-65%, and the average yield of crops can be increased by 15-20%.

The invention discloses application of C8-HSL in promoting nitrogen and phosphorus removal capacity of an algal-bacterial symbiotic system in sewage treatment, the removal of ammonia nitrogen, TN and total phosphorus of the symbiotic system is improved by 25.17%, 36.22% and 38.74% by 0.8 mu M of C8-HSL, and meanwhile, the formation of extracellular polymeric substances (EPS) is increased by 68.62%. Metagenome sequencing results show that the Halomonas and the Sphingobium are main nitrogen and phosphorus removal microorganisms, the narGHI gene and the nirKS gene play a key role in the nitrogen removal process, and the phnX gene and the ppa gene play a key role in the phosphorus removal process. The research provides a theoretical basis and practical guidance for optimizing sewage treatment through a quorum sensing strategy.

The invention discloses a multistage enzyme and quorum sensing signal molecule MABR membrane and a preparation method thereof, through a bionic induction strategy, while the anti-pollution characteristic of the MABR membrane is maintained, multistage enzyme and quorum sensing signal molecules are fixed on the surface of the membrane, and the multistage enzyme and quorum sensing signal molecules induce microorganisms to secrete an extracellular polymeric substance (EPS), so that the binding strength of a biological membrane is improved by 40% or more. The preparation method of the multi-stage enzyme and quorum sensing signal molecule MABR membrane comprises the following steps: (1) screening nitrifying bacteria and extracting multi-stage enzyme and quorum sensing signal molecules of the nitrifying bacteria; (2) carrying out composite pretreatment on the surface of the MABR membrane, wherein the composite pretreatment comprises hydrophilic modification, functional group introduction, swelling of the surface of membrane silk and the like; and (3) immobilizing multistage enzyme and quorum sensing signal molecules (including cell membrane fragments, periplasmic space enzyme complexes and the like) in the strain by adopting an immobilization technology to form a bioactive interface layer with the thickness of about 50-200nm.

The invention discloses a green fluorescence report system for screening a Listeria monocytogenes quorum sensing inhibitor as well as a construction method and application of the green fluorescence report system. The report system is constructed through the following steps: synthesizing a DNA fragment according to a listeria monocytogenes AGR system pII promoter sequence, connecting the DNA fragment with a green fluorescent protein pEGFP plasmid, amplifying an EGFP-pII target fragment through PCR, and connecting the target fragment to a pKSV7 shuttle vector plasmid through homologous recombination to obtain a pKSV7-EGFP-pII plasmid, and then transferring the gene into a competent cell of listeria monocytogenes ATCC BAA-679, and screening to obtain a report system. The report system disclosed by the invention can be used for intuitively evaluating the expression condition of the quorum sensing system of the listeria monocytogenes and screening out the quorum sensing inhibitor.

The invention discloses a cinnamyl aldehyde modified montmorillonite nanosheet capable of inhibiting bacterial quorum sensing as well as a preparation method and application of the cinnamyl aldehyde modified montmorillonite nanosheet. According to the modified nanosheet, a two-dimensional montmorillonite nanosheet is taken as a carrier, and cinnamyl aldehyde is intercalated into an interlayer region of the two-dimensional montmorillonite nanosheet. The modified montmorillonite nanosheet can achieve the effect of inhibiting inflammation by inhibiting bacterial quorum sensing and adjusting the abundance of intestinal microorganisms, effectively relieves symptoms induced by IBD, has good stability, biocompatibility and biodegradability, and can be used for treating inflammatory bowel diseases.

The invention discloses a microbial agent for treating agriculture, forestry, livestock and poultry wastes and a preparation method thereof, the microbial agent is composed of salt-tolerant bacillus, acid-resistant nitrococcus, bacillus amyloliquefaciens and pantoea agglomerans according to a weight part ratio of 3: 2: 1: 0.5, and the microbial agent is subjected to gradient stress-AHLs signal induction domestication. The preparation method comprises the steps of seed culture, synergistic induction culture and microcapsule embedding, active flora synergy is achieved through quorum sensing, and the three-in-one function of degradation, growth promotion and disease resistance is achieved. The microbial agent is high in stress resistance, the shelf life is 12-18 months, the degradation rate of cellulose in 7 days is 65%-75%, the microbial agent is suitable for resource utilization of various agricultural, forestry, livestock and poultry wastes, and a treated product can be directly used as a functional organic fertilizer.

The present invention discloses a polycyclic aromatic hydrocarbon-degrading strain regulated by quorum sensing, a screening method, and an application thereof. The polycyclic aromatic hydrocarbon-degrading strain regulated by quorum sensing is Acinetobacter sp. YY-1, with a deposit number of GDMCC No: 63126 and a deposit date of January 9, 2023. The polycyclic aromatic hydrocarbon-degrading strain provided by the present invention has an enhanced ability to degrade polycyclic aromatic hydrocarbons under quorum sensing control and has good application prospects.

The invention provides Escherichia coli for producing a nylon 12 monomer. The genotypes of the Escherichia coli are as follows: tesA: PtluxR-PluxluxIUcfatB, adhE: PtluxR-PluxluxIUcfatB, fadE: PluxCYP153A-BM3, pgi: PluxCYP153A-BM3, aldB: PluxCYP153A-BM3, and gabD: PM12CV2025BsADH. The invention also provides a preparation method of the Escherichia coli. The genotypes of the Escherichia coli are as follows: tetluxR: PtluxR: PluxluxIUcfatB, adhE: The invention further provides a construction method of the escherichia coli for producing the nylon 12 monomer, a self-induction system in the escherichia coli is constructed by utilizing a Lu xI / R quorum sensing system in vibrio fischeri, and the synthesis rate of AHL is slowed down, so that the self-induction expression of the gene is realized. An engineering escherichia coli strain is constructed based on an established self-induction system, self-induction synthesis of the nylon 12 monomer can be realized, cell growth and product synthesis are balanced, and synthesis of the nylon 12 monomer from glucose is realized.