356 results about "Quorum sensing" patented technology

A urinary catheter clip-on device applies surface acoustic waves of Rayleigh-Lamb and / or Love type to a urinary catheter for preventing biofilms on the catheter surfaces. The bacteria is forced to move relative to the vibrating catheter surface. The amplitudes of bacteria vibrations are in nanometer range. The relative motion of bacteria results in bacteria quorum sensing, and disrupts the bacteria attachment process. The method is preventive as surface acoustic waves create low acoustic energy and bacteria is not killed.

A recombinant phage system has been developed for the rapid detection of bacteria, particularly fecal coliform indicator bacteria. The systems of the invention link phage infection events to quorum sensing signal molecule biosynthesis and bioluminescent bioreporter induction, facilitating the detection of pathogens that may be present in low numbers. The phage-based systems of the invention maintain specificity for the pathogen while still producing significant signal amplification for sensitive and quantitative detection. The systems require only the combination of sample with phage and bioreporter organisms; no extraneous addition of any substrates or user intervention of any kind is necessary, making this approach significantly less technical than standard molecular or immunological methods.

A technique for inhibiting biofouling caused by biofilms grown on a material surface of a water system where microorganisms exist such as the membrane surface of a membrane bioreactor (MBR) for advanced wastewater treatment is disclosed. Specifically, a magnetic carrier comprising an enzyme for quenching quorum sensing that inhibits biofilm formation, a membrane bioreactor system that can be stably operated without inactivation and loss of enzymes and a method for inhibiting biofilm formation are described.

The invention discloses a method for rapidly forming an anammox granule sludge, and belongs to the field of wastewater treatment. Proper amount of an inoculated sludge is added to an SBR (Sequencing Batch reactor), a nutrient solution containing ammonia nitrogen and nitrite is added, the concentrations of both ammonia nitrogen and nitrite in the nutrient solution are both 50 mg / L, an AHLs quorum sensing signal molecule at certain concentration is added into the nutrient solution in the SBR each day, the environment temperature is 32-33 DEG C, the pH value is 7.8-8.3, the DO concentration is 0.15 mg / L or lower, and batch cultivation is conducted. The time for forming the anammox granule sludge is short, and the effect of the granule sludge is good.

The present invention relates to an isolated nucleic acid molecule comprising (a) a first nucleotide sequence encoding a protein that detects the presence, amount or both of a pathogenic microorganism by forming a complex with a quorum sensing molecule produced by said pathogenic microorganism, (b) one or more second nucleotide sequence said one or more second nucleotide sequence being under control of a promoter that is induced by the complex of the protein encoded by the first nucleotide sequence and the quorum sensing molecule produced by said pathogenic microorganism and encoding (i) an antimicrobial peptide, wherein the antimicrobial peptide is effective against the pathogenic microorganism detected by the protein encoded by the first nucleotide sequence; and / or an antibiofilm enzyme wherein the antibiofilm enzyme is effective against the pathogenic microorganism detected by the protein encoded by the first nucleotide sequence; and (c) optionally a third nucleotide sequence encoding a protein that controls the motility of the host organism, wherein the protein that controls the motility of the host organism directs the motility of the host organism towards said pathogenic microorganism. A recombinant microorganism comprising the isolated nucleic acid molecule and a method of sensing and killing pathogenic microorganisms is also described.

The invention belongs to the field of hygiene and relates to a bacillus subtillus degrading bacterial colony sensing signal and use of bacillus subtillus degrading bacterial colony as an antiseptic, in particular to bacillus subtillus degrading gram-negative bacterial colony sensing signal and the formation of a bacterial biofilm of the bacillus subtillus degrading gram-negative bacterial colony. In the use of the degrading bacterial colony sensing signal, a proper concentration of the bacillus subtillus is 5*102 to 5*104cfu / ml. The bacillus subtillus is further used as antiseptic to inhibit the bacteria such as pseudomonas, agrobacterium, enterobacteriaceae and vibrionaceae. The antiseptic can be ointment for external use, liquid preparation for external use, oral tablet, oral liquid, disinfectant, clearing agents or water quality improving agent. The consistency of the bacillus subtillus serving as the antiseptic is 105 to 106 cfu / g. The use of the bacillus subtillus provided by the invention and the antiseptic inhibit the formation of biofilm of disease-causing bacteria and the growth of bacteria by using microbial quorum-sensing inhibition instead of preventing and sterilizing bacteria by compound which is adopted by most of the conventional antiseptic; therefore, the problem of drug resistance in the antimicrobial process is avoided.

Compounds which modulate quorum sensing in quorum sensing bacteria. Compounds of the invention inhibit quorum sensing and / or activate quorum sensing in various bacteria. Compounds that inhibit quorum sensing are particularly useful for inhibition of detrimental bacterial biofilm formation. Compounds that activate quorum sensing are particularly useful for promoting growth and biofilm formation of beneficial bacterial.

The present invention provides compositions and methods for modulating bacterial quorum sensing using antagonist or agonist compounds. Further, the present invention provides methods of treating or preventing microbial damages and diseases, in particular for diseases where there is an advantage in inhibiting quorum sensing regulated phenotypes of pathogens.

The present invention discloses a quorum-sensing signal molecular preparation, which is prepared through the following steps: culturing alpha-proteobacterial, delta-proteobacterial and flavobacteria microorganisms respectively; taking culture solution or supernate made by centrifugalizing culture solution, and mixing with the volume proportion of 1-4:1:1-2; and cooling and drying. The quorum-sensing signal molecular preparation can precipitate nicotine degrading bacteria to colonize and constantly multiply in the contaminated environment, and can precipitate microbial populations in the environment to dynamically develop, so as to effectively improve nicotine removal and the comprehensive treatment efficiency of contaminants. The preparation can treat tobacco waste in cooperation with nicotine degrading bacteria and has the advantage of lasting action with one time of feeding. The preparation can greatly reduce the starting time for an active sludge reactor and accelerate the stabilization of the reactor. The operation is convenient, and both the cost and the maintenance expense can be reduced greatly. Therefore, the present invention provides an ideal treatment method for tobacco waste.

An apparatus and operation method for quickly forming anammox particles using a quorum sensing mechanism are related to the field of wastewater treatment. The invention is directed to an apparatus and operation method for quickly forming particulate sludge via anammox. The apparatus comprises a body reactor, a signal molecular generator, a spherical thin-shell rotary cover, a spherical screen, a manual rotating switch, a ball valve I, a ball valve II, a heating tape, and a temperature control probe. The signal molecular generator is added into the body reactor, signal molecules diffuse in the body reactor so that anammox flocculating sludge quickly forms particulate sludge, and the activity of anammox bacteria is significantly improved while the particulate sludge is formed. The method for implementation according to the invention is simple, has significant effect, is widely applicable to anammox sludge granulation and is effective in improving practical application of anammox process.

Provided is a cell-free expression system and method for its use for detecting the presence of a bacterial biofilm on a surface, wherein the film comprises a) an exogenous quorum sensing protein or an exogenous nucleic acid sequence coding for a quorum sensing protein capable of selectively binding to a bacterial signaling molecule; and b) an exogenous nucleic acid sequence comprising a promoter operably linked to a nucleic acid sequence coding for a marker protein, wherein the promoter is regulated by the binding of the quorum sensing protein to the bacterial signaling molecule. Further provided is an aerosol formulation, as well as an adhesive bandage, each of which may be used for detecting the presence of a bacterial biofilm on a surface, comprising the disclosed cell free expression system and methods therefor.

The invention relates to a construction strategy and a construction process of an unmarked lysobacter enzymogenes engineering strain capable of preventing plant bacteriosis, and belongs to the field of microbial genetic engineering. An excellent exogenous gene aiiA is directionally integrated onto a chromosome, and any marked genes are not brought into the unmarked lysobacter enzymogenes engineering strain. A quorum-sensing system for plant pathogenic bacteria can be efficiently damaged by the engineering strain, pathogenicity of the pathogenic bacteria on a host plant (Chinese cabbage) is remarkably reduced, and biological prevention of the plant bacteriosis is realized.

A urinary catheter clip-on device applies surface acoustic waves of Rayleigh-Lamb and / or Love type to a urinary catheter for preventing biofilms on the catheter surfaces. The bacteria is forced to move relative to the vibrating catheter surface. The amplitudes of bacteria vibrations are in nanometer range. The relative motion of bacteria results in bacteria quorum sensing, and disrupts the bacteria attachment process. The method is preventive as surface acoustic waves create low acoustic energy and bacteria is not killed.

The present invention provides polymer-based slippery liquid-infused porous surfaces (SLIPS) that can prevent adhesion and colonization by fungal and bacterial pathogens and also kill and / or attenuate the colonization and virulence of non-adherent pathogens in surrounding media. The present approach exploits the polymer and liquid oil phases in these slippery materials to sustain the release of small molecules such as a broad-spectrum antimicrobial agent, an antifungal agent, an antibacterial agent, an agent that modulates bacterial or fungal quorum sensing, an agent that attenuates virulence, or a combination thereof. This controlled release approach improves the inherent anti-fouling properties of SLIPS, has the potential to be general in scope, and expands the potential utility of slippery, non-fouling surfaces in both fundamental and applied contexts.

The invention discloses a method for improving activity of anaerobic ammonium oxidation embedded particles and belongs to the field of wastewater treatment. Quorum sensing signal molecules of AHLs are added to seed sludge when anaerobic ammonia oxidation seed sludge is cultured and acclimatized, the cultured sludge is mixed with a PVA-SA (polyvinyl acetate-sodium alginate) embedding agent, signal molecules are added again in the mixing process, and the PVA-SA anaerobic ammonium oxidation embedded particles are prepared after the mixture is crosslinked for several hours. With the adoption of the method, the activity is substantially improved, the preparation process is simple, the operation is convenient and the performance is stable.

Methods for selectively manipulating a growth rate of a selected bacterium comprising the step of contacting the selected bacterium with a predetermined amount of a quorum sensing molecule to affect a change in the growth rate of the selected bacterium, wherein the quorum sensing molecule is species specific, and the change in the growth rate is dependent on the amount of quorum sensing molecule in a dose-dependent fashion. Also provided are methods for treating or protecting against bacterial infections by utilizing the dose-dependent response of bacterial quorum sensing systems. The methods can be applied to a wide range of bacteria species including Streptococci, Staphylococci, and Bacilli. Compositions, medicaments and oral formulations for use with the methods are also disclosed.

The invention provides methods for identifying and analyzing modulators of quorum-sensing signaling in bacteria.

The invention discloses a method for extracting triterpene substances from inonotus obliquus by use of quorum sensing molecules induction, wherein the method comprises the following steps: adding activated inonotus obliquus CFCC 83414 into a liquid medium, and performing fermentation culture for 8-14 days at 24-26 DEG C; adding quorum sensing molecules in the 5th-8th days of the fermentation; after the fermentation, separating thalli and fermentation liquid; performing aftertreatment to separate and purify triterpene substances from the thalli and fermentation liquid. According to the method disclosed by the invention, quorum sensing molecules are added in the fermentation metaphase to stimulate growth of inonotus obliquus; the method does not need introduction of a gene engineering strainor additional enzyme, is simple in aftertreatment, and can effectively increase the yield of triterpene substances in inonotus obliquus.

The invention discloses an urban wastewater composite disinfection preparation and an application thereof. The preparation is prepared from the following components in percentage by weight: 20-40 percent of composite quorum sensing inhibitor, 20-40 percent of sodium hypochlorite, 20-30 percent of surfactant, 10-20 percent of inorganic polymer coagulant, and 0-20 percent of composite solubilizer. The preparation is prepared by compounding the quorum sensing inhibitor serving as a main component with the sodium hypochlorite, the surfactant and the coagulant with disinfection, sterilization, coagulation and other functions, can be used in further treatment of urban wastewater, can be used for improving the effects of disinfection and sterilization, and is safer, more environment-friendly and more effective.

The invention relates to a strain of bacillus subtilis capable of efficiently degrading quorum sensing signal molecules and a low cost production method and application method of the inoculant of bacillus subtilis, and belongs to the technical fields of agricultural microbial inoculant and energy saving and emission deduction. The adopted strain is a SZX11 strain of bacillus subtilis. The SZX strain is separated and screened from pine needles, and is preserved in China General Microbiological Culture Collection Center (CGMCC). The preservation number is CGMCC No.8188. Sweet potato starch wastewater is taken as the liquid fermentation medium to massively produce bacillus subtilis and prepare agricultural microbial inoculants; the technology has the advantages of low energy consumption, low production cost, and simple technology, moreover, the sweet potato starch wastewater is utilized, and the pollutant discharge is reduced. The produced inoculant can be added into the irrigation water of irrigation system, is capable of reliving the obstruction of irrigation devices, can also improve the quality of agricultural products, and has a wide application prospect.

The invention discloses an agent for soil antibiotics resistance gene contamination reduction and preparation and application of the agent. A preparation method of the agent comprises the following steps that (1) at least one plant of dandelions, fig leaves, pineapple peels, roses, geraniums, lavender, pea hulls and rosemary is aired and then crushed, filtering is carried out after extraction, filter liquor and plant residues are collected, and the filter liquor is subjected to rotary evaporation to obtain natural plant crude extracts; and (2) 2-hydroxy-1,2-di-2-pyridylethanone, 2,5-diketopiperazine and the natural plant crude extracts are uniformly mixed according to the ratio to obtain a quorum sensing inhibitor, the plant residues and carbamide are mixed according to the ratio to obtain a soil improvement agent, the quorum sensing inhibitor and the soil improvement agent are uniformly mixed according to the ratio, and the agent can be obtained. The agent is directly applied to antibiotics resistance gene contaminated soil, or the agent is applied to the antibiotics resistance gene contaminated soil after being uniformly mixed with fertilizer, or the agent is applied to the antibiotics resistance gene contaminated soil before the fertilizer is applied.

The invention relates to the field of analytical chemistry, and discloses a method for simultaneously determining bacterial quorum sensing AHLs molecules. A to-be-detected sample is added in a high performance liquid chromatography-tandem mass spectrometer for gradient elution. The elution conditions are that the elution is carried out by using an A phase (methanol containing 10 mmoI / L ammonium acetate and 0.2 % formic acid) and a B phase (a water solution containing 10 mmoI / L ammonium acetate and 0.2 % formic acid); parameters for the gradient elution are as follows: at 0 min, the content of the A phase is 20% of and the content of the B phase is 80% and the content reaches a preset proportion at the moment; from 0 min to 8 min, two flowing phases are changed into 100% of the A phase and 0% of the B-phase at an average rate; from 8 min to 10.1 min, the content of the A phase is 100% of and the content of the B phase is 0%; at 10.1 min, the content of the A phase is adjusted to 20% of and the content of the B phase adjusted to 80% instantly; and elution is continuously performed to 15 min. The method has good linear relationship, a detection unit of 0.1-1.0 ng / L, has high accuracy and sensitivity, and simple operations.

The present invention provides a control agent that is extremely effective in controlling bacterial diseases. The quorum sensing inhibitor and bacterial disease control agent for agricultural and horticultural use of the present invention contain a pyrimidinone compound represented by General Formula (1):wherein R1 represents a C1-5 alkyl group or a phenyl group, and R2 represents a hydrogen atom or a C1-5 alkyl group, provided that R1 cannot be a methyl group when R2 represents a hydrogen atom.

The invention discloses Acinetobacter lactucae and an application thereof to degradation of a quorum-sensing signal molecule DSF (diffusible signal factor). The invention provides a bacterial strain of Acinetobacter lactucae QL-1, the bacterial strain is preserved in a China Center for Type Culture Collection on September 11th, 2017, and the preservation number is CCTCC NO: M2017487. The bacterialstrain can employ the quorum-sensing signal molecule DSF as a sole carbon source for growth, and 2mM of DSF signal molecules can be completely degraded in 15 hours with high degradation activity andstable degradation performance. The bacterial strain QL-1 has substantial biocontrol effects for black rot caused by Xanthomonas campestris XC1, and the bacterial strain also has characteristics of fast growth speed, simple cultivation method, good adaptation capability without easy variation, and great application potential for promotion of control of pathogens which are pathogenic depending on DSF; and at the same time, the product can reduce the problem of abuse of pesticides, and provides a new thinking for biological control of plant diseases.

Compounds which modulate quorum sensing in quorum sensing bacteria. Compounds of the invention inhibit quorum sensing and / or activate quorum sensing in various bacteria. Compounds that inhibit quorum sensing are particularly useful for inhibition of detrimental bacterial biofilm formation. Compounds that activate quorum sensing are particularly useful for promoting growth and biofilm formation of beneficial bacterial.

The present invention relates to the use of compounds of the general Formula (XIII): In particular the invention refers to a family of compounds that block the quorum sensing system of Gram-negative bacteria, a process for their manufacture, pharmaceutical compositions containing them and to their use for the treatment and prevention of microbial damages and diseases, in particular for diseases where there is an advantage in inhibiting quorum sensing regulated phenotypes of pathogens.

The invention relates to antibacterial application of small molecular bacterial quorum sensing inhibitor Falcarindiol in the condition of no inhibition on bacteria growth concentration (subinhibitory concentration). The active compound has a broad-spectrum bacterial quorum sensing resistant activity, can inhibit bacterial virulence in subinhibitory concentration, reduces the harm of bacteria to the host, and can prevent and treat quorum sensing bacterial infections. The compound can play the role of inhibition of bacterial virulence in subinhibitory concentration, so that pressure for survival of pathogenic bacteria is not brought, drug resistance is not easy to produce, the antibacterial application of the small molecular bacterial quorum sensing inhibitor Falcarindiol in the condition of no inhibition on bacteria growth concentration (subinhibitory concentration) is different from application of killing bacteria and bacteria growth inhibition of other antibacterial agent, is also is different from the antibacterial application of bacteria growth inhibition of Falcarindiol with the bacteria concentration (the use final concentration is greater than the minimum bacteriostasis concentration), and is a new antibacterial application. The antibacterial application of the small molecular bacterial quorum sensing inhibitor Falcarindiol in the condition of no inhibition on bacteria growth concentration (subinhibitory concentration) has the advantages that: raw materials are easy to get, compound separation and purification are simple and easy to control, security is high, use concentration is low, and drug resistance is easy to produce, and the like, so that the Falcarindiol can be used in preparation of a new antibacterial agent.

A technique for inhibiting biofouling caused by biofilms grown on a material surface of a water system where microorganisms exist such as the membrane surface of a membrane bioreactor (MBR) for advanced wastewater treatment is disclosed. Specifically, a magnetic carrier comprising an enzyme for quenching quorum sensing that inhibits biofilm formation, a membrane bioreactor system that can be stably operated without inactivation and loss of enzymes and a method for inhibiting biofilm formation are described.

The present invention relates to the use of compounds as broad spectrum inhibitors of bacterial biofilm formation. In particular the invention refers to a family of compounds that block the quorum sensing system of Gram-negative and Gram-positive bacteria, a process for their manufacture, pharmaceutical compositions containing them and to their use for the treatment and prevention of bacterial damages and diseases, in particular for diseases where there is an advantage in inhibiting quorum sensing regulated phenotypes of pathogens.

The invention discloses an iterative gene circuit based on a vibrio fischeri quorum sensing system and a T7 expression system. The iterative gene circuit comprises a signal molecular protein gene luxIof vibrio fischeri, a receptor protein gene luxR, a promoter sequence PluxI(1) for sensing a signal molecular and receptor protein complex, a T7 RNA polymerase gene T7 RNApoly and a green fluorescentprotein egfp for representing the circuit intensity. The gene circuit does not rely on an inducer and can utilize the T7 expression system to spontaneously and forcefully initiate target gene expression in any host bacterium; and gene elements are constructed on different expression vectors, the iterative gene circuit is constructed in engineering bacteria, the T7 expression system is utilized toamplify signals of the vibrio fischeri quorum sensing system, and the iterative gene circuit has the function of utilizing the T7 expression system to spontaneously and forcefully initiate target gene expression, and has a lower leakage ratio and higher initiating strength.