15 results about "Bakuchiol" patented technology

The invention belongs to the field of biosynthesis, and particularly relates to saccharomyces cerevisiae engineering bacteria for producing psoralen as well as a construction method and application of the saccharomyces cerevisiae engineering bacteria. The saccharomyces cerevisiae engineering bacteria comprise a yield increasing path of DMAPP, a synthetic path of naringenin, a synthetic path of daidzein and a synthetic path of psoralen; the yield increasing approach of the DMAPP is to express mvaE, mvaS, ERG12, ERG8, ERG19 and IDI1 on a saccharomyces cerevisiae strain WAT11, and to express mvaE, mvaS, ERG12, ERG8, ERG19 and IDI1 on a saccharomyces cerevisiae strain WAT11. The synthetic route of the naringenin is that PAL, 4CL, CHS and CHI are expressed in Saccharomyces cerevisiae with yield increased by dimethyl allyl pyrophosphoric acid; the synthetic route of daidzein is that CHR, IFS and HID are expressed in saccharomyces cerevisiae synthesized by naringenin; the synthetic route of the psoralen is that PT and OC are expressed in saccharomyces cerevisiae synthesized by daidzein.

A topical neurocosmetic composition comprising a first component including one or more of bakuchiol, allantoin, squalane, or Morinda citrifolia extract; a second component including one or more of hyaluronic acid, vitamin E, Zanthoxylum alatum fruit extract, Coriandrum sativum seed oil, Vitex agnus-castus extract, Helianthus annuus sprout extract, niacinamide, resveratrol, or an ascorbyl ester; and a cosmetically acceptable oil-in-water vehicle configured to deliver both lipophilic and hydrophilic actives through the skin to dermal neurons. The composition improves measurable skin parameters such as hydration and elasticity and supports psychophysiological comfort. Methods of manufacture and use are also described

The application belongs to the technical field of cosmetics, and particularly relates to a whitening and anti-aging composition containing psoralen and vitamin C and a preparation method thereof. The whitening and anti-aging composition provided by the application is composed of scutellaria baicalensis georgi extract, white pool flower seed oil, psoralen and vitamin C, has a rapid and efficient whitening and anti-aging effect, is mild and non-irritating, and can be used for preparing skin care products with whitening and anti-aging effects. In the preparation of the scutellaria baicalensis georgi extract, a deep eutectic solvent combined with a pulsed direct current field treatment is used, which has the advantages of low cost, high extraction efficiency, simple operation, non-toxicity, good biocompatibility, biodegradability and the like. The preparation method of the whitening and anti-aging composition provided by the application is simple and easy to operate, is beneficial to industrialized production and application, and has good market application value.

The application discloses a Pichia pastoris engineering bacterium for converting methanol to synthesize bakuchiol from scratch as well as construction and application of the bacterium. In different chromosomal neutral sites of the Pichia pastoris, a heterologous p-coumaric acid synthesis pathway is introduced, key genes of tyrosine and phenylalanine synthesis pathways of a heterologous shikimic acid pathway are knocked out, overexpressed or heterologously expressed, a high-yield p-coumaric acid strain is obtained, on the basis of the strain, a heterologous bakuchiol biosynthesis pathway is introduced, bakuchiol synthetase is overexpressed, key genes of a MVA pathway and genes of an exogenous acetyl coenzyme A supply pathway are endogenously overexpressed or heterologously expressed, after optimization of a methanol concentration and back complementation of a His4 gene of the strain, the yield of the bakuchiol is effectively increased to 91.2 mg / L, which is 59.8 times higher than that of an initial strain, and the yield of the bakuchiol in a 15L fermenter reaches 692.8 mg / L. The application has the characteristics of high conversion efficiency, low production cost, easy preparation, wide industrial application prospect and the like.

This invention belongs to the field of cosmetic technology, specifically relating to a firming, brightening, and soothing composition and its application. The firming, brightening, and soothing composition provided by this invention is a compound of *Palmaria rubra* bark extract, *Capsella bursa-pastoris* fruit extract, *Saururus chinensis* extract, and bakuchiol. These four ingredients synergistically promote the transdermal absorption of active ingredients, achieving firming, brightening, and soothing effects. Furthermore, this composition is gentle and non-irritating, and its preparation method is simple, making it feasible for industrialization. The composition provided by this invention has a wide range of applicability in skincare products. Its preparation method for serums is simple and easy to operate, and the prepared serums have firming, brightening, and soothing effects. Moreover, patch tests show that the serums are gentle and non-irritating, indicating that the composition of this invention has broad market application value in skincare products.

The invention discloses cactus essence oil with an anti-wrinkle effect and a preparation method of the cactus essence oil, and belongs to the technical field of skin care. Comprising the following raw materials: prickly pear seed oil, simmondsia chinensis seed oil, squalane, caprylic / capric triglyceride, bakuchiol, rosa damascena flower oil, sandalwood oil, frankincense oil, carrot seed oil, pelargonium graveolens oil, bisabolol, crithmum maritimum oil and tocopherol. According to the cactus essence oil with the anti-wrinkle effect and the preparation method of the cactus essence oil, the cactus seed oil is used as a core substrate, core components such as bakuchiol and crithmum maritimum oil are scientifically matched with soothing components such as bisabolol, and special preparation processes such as low-temperature premixing, step-by-step mixing, vacuum homogenization and light-shielding standing are combined, so that the cactus essence oil with the anti-wrinkle effect is obtained. The prepared cactus essence oil realizes multiple improvements of wrinkle resistance, stability maintenance, skin feeling and production practicability.

The invention belongs to the field of plant active matter extraction equipment, relates to a continuous extraction device for improving the yield of bakuchiol and application of the continuous extraction device, and aims to solve the problems of long retention time, low solid-liquid mass transfer efficiency and low product yield of an extraction process in an existing bakuchiol extraction technology. The device comprises a jet flow slurry state kettle, a rising type stirring and sinking type jet flow system integrated in the kettle, a circulating pump integrated outside the kettle and a heater, continuous feeding is achieved, and continuous discharging is achieved through a solid-liquid separator. The rising type stirring and sinking type jet flow system of the device realizes the effects of countercurrent mixing and turbulence enhancement in the kettle, fresh fed materials and circulating materials are fully and uniformly mixed, the solid-liquid mass transfer efficiency is greatly improved, the extraction time is shortened, and the extraction yield of bakuchiol is improved; and meanwhile, continuous feeding and continuous discharging are achieved, and the production efficiency of the device is greatly improved. Compared with the prior art, the product yield is increased by 10% or above, the production efficiency is improved by 50% or above, and the method has the advantages of being adjustable in production scale, high in yield, easy to operate, continuous, low in cost and the like.

The invention discloses an isopentenyl transferase and an application of the isopentenyl transferase in synthesis of bakuchiol. Specifically, recombinant saccharomyces cerevisiae for expressing the isopentenyl transferase is constructed, the isopentenyl transferase CcPT6 from fructus psoraleae is obtained through screening, the isopentenyl transferase CcPT6 can catalyze an isopentenylation reaction of a coumaric acid substrate to generate bakuchiol, and the activity of the isopentenyl transferase CcPT6 is higher than that of a reported sequence BAK36. The CcPT7 is obtained by truncating 54 amino acids at the N end of the CcPT6, the catalytic activity is improved by 74%, and the activity of the CcPT7 is higher than that of a reported BAK36 mutant. In addition, the invention also provides a recombinant saccharomyces cerevisiae and yarrowia lipolytica engineering strain for integrally expressing the isopentenyl transferase CcPT6 or CcPT7, so that the artificial biosynthesis of the bakuchiol is realized. The discovery of the isopentenyl transferase and the construction of the recombinant engineering strain have important meanings for promoting the biosynthesis of the bakuchiol.

The application provides a recombinant yeast for producing bakuchiol, a construction method and application thereof. The recombinant yeast is Yarrowia lipolytica containing isopentenyl transferase, and a gene coding the isopentenyl transferase is derived from Psoralea corylifolia Linn. The recombinant yeast can be used to produce bakuchiol in a large yield through a biosynthetic method, and provides technical support for industrialization of the bakuchiol.

The invention discloses an application of psoralen B in preparation of a medicine for treating helminthosis, an in-vitro effectiveness experiment result of psoralen B on an echinococcosis multilocularis protoscolex shows that the killing effect of maduramicin ammonium on the protoscolex has concentration dependence, the death rate of the protoscolex can reach 90.63% + / -0.96% after the maduramicin ammonium is treated for 6 h at the concentration of 50 mu M, and the death rate of the protoscolex can reach 90.63% + / -0.96% at the concentration of 100 mu M, and the death rate of the maduramicin ammonium can reach 90.63% + / -0.96% at the concentration of 100 mu M. The death rate of the original head section can directly reach 100%; meanwhile, in-vitro effectiveness experiment results of psoralen B on trichina at different development stages show that after the trichina is treated for 72 hours under the concentration of 50 mu M, 80 mu M and 100 mu M, the death rates of muscular larvae reach 89.44% + / -0.81%, 96.29% + / -1.30% and 96.68% + / -1.06% respectively; after 48 hours of treatment, the death rate of the adults is 100%, and the killing effect is remarkable. More importantly, the applicant verifies that IBC can directly destroy the epidermal structure and cell integrity of the insect body for the first time through observation by an optical microscope and an electron microscope, and the discovery provides intuitive morphological evidence for the anti-parasitic effect of IBC.

The application belongs to the field of biosynthesis and particularly relates to a Saccharomyces cerevisiae engineering bacterium for producing bakuchiol, a construction method and application thereof. The Saccharomyces cerevisiae engineering bacterium comprises a DMAPP production pathway, a naringenin synthesis pathway, a daidzein synthesis pathway and a bakuchiol synthesis pathway. The DMAPP production pathway is expressed by mvaE, mvaS, ERG12, ERG8, ERG19 and IDI1 in the Saccharomyces cerevisiae strain WAT11. The naringenin synthesis pathway is expressed by PAL, 4CL, CHS and CHI in the Saccharomyces cerevisiae strain with increased dimethylallyl pyrophosphate. The daidzein synthesis pathway is expressed by CHR, IFS and HID in the Saccharomyces cerevisiae strain with naringenin synthesis. The bakuchiol synthesis pathway is expressed by PT and OC in the Saccharomyces cerevisiae strain with daidzein synthesis.

The invention discloses a firming and anti-wrinkle composition as well as a preparation method and application thereof, and the firming and anti-wrinkle composition comprises the following components in parts by mass: 0.2-0.5 part of a growth factor accelerant, 1 part of a lindera glauca fruit extract and 0.02-0.08 part of salmon essence source DNA (deoxyribonucleic acid) sodium. The growth factor accelerant is selected from at least one of hydroxyl pinacolone retinoate, bakuchiol, a spanishneedles herb extract, asiaticoside, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7. The growth factor accelerant, the lindera glauca fruit extract and the salmon essence source DNA sodium cooperate to improve the problems of skin laxity and slowdown epidermal layer cell renewal caused by deep protein loss and wrinkle formation caused by decline of the stratum corneum moisturizing capacity, and the effects of tightening and resisting wrinkles are synergistically exerted in multiple aspects.

The application discloses a brewing yeast engineering strain for producing bakuchiol as well as a construction method and application thereof. The engineering strain overexpresses a gene in a geranyl pyrophosphate synthesis pathway, a gene in a p-coumaric acid synthesis pathway and a mutant isopentenyl transferase PcPT07 coding gene; the engineering strain has the ability to synthesize bakuchiol from glucose as a single carbon source; the classification and naming of the engineering strain is Saccharomyces cerevisiae BAK07, and the preservation number is CGMCC NO.31081. The application realizes the de novo synthesis of bakuchiol in the brewing yeast through overexpression of the gene in the geranyl pyrophosphate synthesis pathway, the gene in the p-coumaric acid synthesis pathway and the mutant isopentenyl transferase PcPT07 and combining metabolic engineering and other strategies, and lays a foundation for producing bakuchiol through microbial fermentation.

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-aging, firming, soothing and repairing composition and a preparation method thereof. The anti-aging, firming, soothing and repairing composition provided by the invention comprises an anti-aging and firming component and a soothing and repairing component, the anti-aging and firming component is prepared from a fructus schisandrae chinensis extract, a flos chrysanthemi extract, a radix arnebiae extract and bakuchiol; the soothing and repairing component is prepared from squalane, flat nuclear wood oil and lavender oil. The composition has excellent anti-aging and soothing effects, is mild and non-irritating, and can effectively improve the elastinase inhibition rate and the hyaluronidase inhibition rate. The composition provided by the invention can be applied to skin care products such as essence and the like, and is wide in applicable object range, simple and easy to operate in preparation method and suitable for actual production; the essence can also remarkably improve the skin compactness value and the skin plasticity elasticity value, and it is indicated that the composition has good application value in skin care products.

The invention provides a preparation method of a composition for improving the activity of bakuchiol and application of the composition in products for delaying skin aging, and belongs to the field of cosmetic additives. According to the preparation method of the saponin composition, the adopted strain is lactobacillus tarda, and the saponin composition is prepared through the steps of inoculation, fermentation culture, induced enzyme production, conversion and post-treatment. In the step of inducing enzyme production, an inducer is added into the fermentation tank, and the adding amount of the inducer is 1-2% of the mass of the basic culture medium. And mixing the prepared saponin composition powder with bakuchiol to prepare the product for delaying skin aging. Capsaicin receptors and metal matrix protease in cells can be effectively inhibited, and the expression level of sirt1 is improved.