64 results about "Psoralen" patented technology

The invention discloses a pichia pastoris engineering bacterium for synthesizing bakuchiol from de novo by converting methanol as well as construction and application of the pichia pastoris engineering bacterium. A heterologous p-coumaric acid synthesis pathway is introduced to neutral sites of different chromosomes of pichia pastoris, a high-yield p-coumaric acid strain is obtained through gene knockout, overexpression or heterologous expression of tyrosine and phenylalanine synthesis pathway key genes of a shikimic acid pathway, a heterologous bakuchiol biosynthesis pathway is introduced on the basis, and a high-yield p-coumaric acid strain is obtained. After overexpression of bakuchiol synthetase, endogenous overexpression or heterologous expression of MVA pathway key genes and exogenous acetyl coenzyme A supply pathway genes, methanol concentration optimization and bacterial strain His4 gene supplementation, the yield of bakuchiol is effectively increased to 91.2 mg / L and is increased by 59.8 times compared with that of an initial bacterial strain, and the yield of bakuchiol in a 15L fermentation tank reaches 692.8 mg / L. The method has the characteristics of high conversion efficiency, low production cost, convenience in preparation, wide industrial application prospect and the like.

The application provides a method for determining components in Zhichun capsules, and simultaneously determines the contents of four components, i.e., isopsoralen, psoralen, icariin and cimicifugan, in Zhichun capsules by using high performance liquid chromatography-ultraviolet detection. By reasonably selecting chromatographic conditions and detection conditions, the method can realize rapid extraction and detection of the four components in Zhichun capsules, and has the advantages of simplicity, high sensitivity, fast analysis speed and the like, and can be used for quality control of Zhichun capsules.

The invention discloses a green method for preparing bakuchiol and application of bakuchiol in oxidation resistance. The invention provides a green method for preparing bakuchiol, aiming at the problems of low extraction rate, large loss of active ingredients, harm to health due to solvent residue, complicated extraction process and the like in the current bakuchiol extraction process, a deep eutectic solvent is used as an extraction medium, and a fructus psoraleae extract is extracted under the assistance of ultrasonic waves. The bakuchiol is further obtained through macroporous resin purification and separation, and the bakuchiol prepared by the method has remarkable antioxidant activity. According to the preparation method provided by the invention, the extraction content of the bakuchiol is remarkably increased, the problems existing in a traditional extraction process are solved, compared with a traditional organic solvent extract, the preparation method is simple and easy to operate, and the prepared bakuchiol is high in content, better in biological activity, green, safe, free of solvent residues and suitable for industrial production. The compound can be used for preparing more antioxidant drugs.

The present invention discloses a novel method of isolating pure bakuchiol, substantially free of furanocoumarins (psoralen and isopsoralen) from the seeds of Psoralea sp using a combination of processes that include supercritical fluid extraction, high vacuum distillation and column purification. The invention also disclose compositions comprising bakuchiol that is free of furanocoumarins.

The present application relates to a hybridoma cell strain secreting psoralen and isopsoralen monoclonal antibody. The hybridoma cell strain of the present application is preserved in China General Microbiological Culture Collection Center on April 18, 2024, the preservation address is No. 1, Xibaixili, Chaoyang District, Beijing, and the preservation number is CGMCC NO. 45909. The psoralen and isopsoralen monoclonal antibody secreted by the hybridoma cell strain of the present application has good sensitivity and specificity to psoralen and isopsoralen, so that the psoralen and isopsoralen can be efficiently and rapidly detected.

This invention provides a chalcone isopentenyltransferase gene. GiPT16 This invention relates to the GiPT16 protein, amplification primer set, and applications, belonging to the field of biogenetics technology. It is based on *Glycyrrhiza inflata* (GiPT16 protein, amplification primer set, and applications). Glycyrrhiza inflata Using whole-genome sequencing data and a reverse genetics strategy, the key aromatic isopentenyltransferase GiPT16, involved in the isopentenylation modification of chalcone active ingredients, was successfully identified and functionally characterized. This enzyme was confirmed to specifically catalyze the biosynthesis of psoralen and glycyrrhizin C using DMAPP as a donor. It is the first chalcone isopentenyltransferase characterized in *Glycyrrhiza inflata*, filling a gap in the study of key enzymes in the chalcone isopentenylation metabolic pathway of this species and providing important evidence for further elucidating the molecular mechanisms of quality formation in *Glycyrrhiza inflata* medicinal materials.

The application discloses a psoralen derivative or a pharmaceutical salt thereof, and a structure general formula of the psoralen derivative is shown in the following formula: The psoralen derivative is obtained by optimizing the structure of psoralen, and the antibacterial activity test is carried out on common gram-positive bacteria such as drug-resistant staphylococcus aureus and gram-negative bacteria such as escherichia coli and klebsiella pneumoniae, and it is found that the compound or the pharmaceutical salt thereof has excellent anti-drug-resistant bacteria, anti-inflammatory and anti-tumor activities, and has good development value in preparation of anti-infection treatment drugs, anti-inflammatory drugs and anti-tumor drugs.

This invention proposes the application of psoralen as an Nrf2 agonist in drug preparation. This invention is the first to discover that psoralen is a specific Nrf2 agonist. Psoralen can directly target and bind to the Nrf2 protein, improving its stability, promoting its phosphorylation and nuclear translocation, thereby activating the Nrf2 signaling pathway, upregulating the expression of downstream antioxidant, anti-inflammatory, and anti-ferroptosis-related proteins, and achieving cell protection and tissue repair functions.

The invention discloses a pharmaceutical composition for inhibiting fusarium graminearum and application of the pharmaceutical composition, and belongs to the technical field of pharmaceutical compositions. The invention provides a composition with fusarium graminearum inhibitory activity. The composition is prepared from two or three compound monomers of osthole, isopsoralen and xanthoxylin. The inhibition rate of each composition to fusarium graminearum is higher than that of each monomer, and the components in the composition have a synergistic effect, so that the composition has important application value in the field of prevention and treatment of wheat scab.

The present invention discloses a natural raw material synergistic blood pressure lowering health-care tea drink and a preparation method thereof, and relates to the technical field of health-care tea drinks, the raw materials comprise, by weight, 50 parts of radix glehniae, 20 parts of radix angelicae and 20 parts of fructus psoraleae, and the auxiliary materials comprise, by weight, 3 parts of sodium benzoate, 3 parts of sodium cyclamate, 2 parts of chitosan, 2 parts of xanthan gum, 0.1 part of lemon yellow and 0.1 part of carmine. According to the blood pressure-reducing health-care tea drink with natural raw materials synergistic interaction, the synergistic interaction among the raw materials is realized through the matching effect among the radix glehniae, the fructus psoraleae and the radix angelicae, the radix glehniae has the effects of reducing blood pressure and blood fat, improving digestion, improving sleep and the like, and the radix angelicae has the effects of reducing blood pressure, relieving exterior syndrome, dispelling wind, relieving pain, eliminating dampness, arresting leucorrhea, diminishing swelling, expelling pus and the like; the psoralen has pharmacological effects of strengthening heart, resisting osteoporosis, resisting tumors, resisting oxidation, protecting nerves and the like, and the active component methoxysarin is extracted and added into the tea drink, so that the tea drink can efficiently reduce blood pressure and improve blood circulation.

The embodiment of the invention provides PSO / PTX targeted composite nanoparticles as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a phospholipid composition in a first organic solvent, adding water and an emulsifier after dissolving, and uniformly mixing to obtain a water phase; the phospholipid composition comprises phospholipid and modified phospholipid, dissolving PLGA in a second organic solvent, adding PTX, PSO and SPIONs (at) OA after dissolving, and uniformly mixing to obtain an organic phase; and adding the organic phase into the water phase, carrying out ultrasonic emulsification, evaporating to remove the organic solvent, and filtering to obtain the PSO / PTX targeted composite nanoparticles. According to the PSO / PTX targeted composite nanoparticles prepared by the embodiment of the invention, the fact that psoralen and paclitaxel are co-loaded on the superparamagnetic polymer lipid nanoparticles is proposed for the first time, and the PSO / PTX targeted composite nanoparticles have the functions of magnetic targeting, long circulation and slow release, so that the PSO / PTX targeted composite nanoparticles have a good application prospect in preparation of the medicines for treating the breast cancer.

The application belongs to the technical field of cosmetics, and particularly relates to a whitening and anti-aging composition containing psoralen and vitamin C and a preparation method thereof. The whitening and anti-aging composition provided by the application is composed of scutellaria baicalensis georgi extract, white pool flower seed oil, psoralen and vitamin C, has a rapid and efficient whitening and anti-aging effect, is mild and non-irritating, and can be used for preparing skin care products with whitening and anti-aging effects. In the preparation of the scutellaria baicalensis georgi extract, a deep eutectic solvent combined with a pulsed direct current field treatment is used, which has the advantages of low cost, high extraction efficiency, simple operation, non-toxicity, good biocompatibility, biodegradability and the like. The preparation method of the whitening and anti-aging composition provided by the application is simple and easy to operate, is beneficial to industrialized production and application, and has good market application value.

The application discloses application of psoralen in preparation of a medicine for treating sepsis. As a natural product, the psoralen has high safety and good tolerance, and can intervene in a pathological process of the sepsis through various action mechanisms such as inhibition of an inflammatory reaction, regulation of an immune reaction and promotion of tissue repair. When the psoralen is applied to treatment of the sepsis, the inflammatory reaction of the sepsis can be effectively controlled, tissue damage can be reduced and the mortality rate can be lowered, so that the survival rate and the life quality of the sepsis patients can be improved.

The present invention discloses a psoralen mannosylated chitosan polymer nanoparticle and its preparation method and application. The psoralen is composed of psoralen, poly(lactic-co-glycolic acid) copolymer, mannosylated chitosan and polyvinyl alcohol, wherein the mass ratio of psoralen, poly(lactic-co-glycolic acid) copolymer, mannosylated chitosan and polyvinyl alcohol is 2-10:10-30:16.5-38.5:1-9. The psoralen mannosylated chitosan polymer nanoparticle is composed of free psoralen drug and mannosylated chitosan polymer carrier. Compared with traditional psoralen, the psoralen has a more stable structure and better physicochemical properties, and has higher bioavailability and tumor targeting. It can be used as a regulator of tumor-associated macrophages and cytokines, and can also inhibit the growth of MDA-MB-231 breast cancer cells and can be used to prepare a drug for treating triple-negative breast cancer.

The invention discloses a method for determining chemical components in a medicine for treating vitiligo, which relates to the technical field of medicine analysis, and is characterized in that ultra-high performance liquid chromatography-mass spectrometry is adopted to determine the content of chemical components in a Bailing tablet: psoralen, isopsoralen, astragaloside, quercetin, polysaccharide and amino acid. The determination method comprises the following steps: respectively injecting a reference solution and a test solution into a liquid chromatograph for determination to obtain a chromatogram, and calculating the content of paeoniflorin in a test sample by adopting an external standard method, chromatographic conditions of a chromatogram obtained by adopting a liquid chromatograph are as follows: octadecylsilane chemically bonded silica is taken as a filling agent. According to the method for determining the chemical components in the medicine for treating leucoderma, the established detection method is verified, and the result shows that the detection method is easy and convenient to operate, accurate in result, high in specificity and good in reproducibility.

The present invention belongs to the technical field of corn bacterial wilt prevention and control, and specifically relates to a fungicide composition for preventing and controlling corn bacterial wilt. The fungicide composition comprises a compound of psoralen and zoxamide or silthiopyrad as active ingredients. The fungicide composition, which combines psoralen with zoxamide or silthiopyrad, exhibits a co-toxicity coefficient greater than 120 against corn bacterial wilt pathogens, demonstrating a synergistic effect. Compared to single zoxamide or silthiopyrad, the control efficacy is significantly improved.

The present invention provides a method for constructing the HPLC characteristic chromatogram of Chuanminshen medicinal materials, cut pieces, standard decoctions and their formula granules, comprising: A) extracting the Chuanminshen raw materials with a solvent to obtain a test solution; B) determining the test solution by high performance liquid chromatography to obtain the HPLC characteristic chromatogram of the Chuanminshen raw materials; the chromatographic conditions of the high performance liquid chromatography are as follows: the chromatographic column is a C18 column; mobile phase A is acetonitrile, and mobile phase B is 0.1% phosphoric acid, with gradient elution. The present invention adopts high performance liquid chromatography, selects acetonitrile - 0.1% phosphoric acid as the mobile phase for gradient elution, uses psoralen as the reference substance, and establishes the HPLC characteristic chromatogram of Chuanminshen medicinal materials, cut pieces, standard decoctions and their formula granules, which has good repeatability and precision, and the method is stable and reliable, and can control the quality of Chuanminshen medicinal materials, cut pieces, standard decoctions and their formula granules.

The application relates to the field of medicines, and discloses a medicine combination for preventing and treating osteoporosis syndrome and a preparation method, wherein the medicine combination is made of tortoise shell, deer horn, psoralea, drynaria and rhizoma curculiginis. A stepwise liquid-phase assisted co-grinding technology is adopted, that is, psoralen extract, deer horn coarse powder and part of tortoise shell coarse powder are ground at low temperature of 0-5 DEG C, psoralen is locked in an amorphous solid dispersion state by using the keratin network of the deer horn; then the remaining tortoise shell coarse powder and drynaria-rhizoma curculiginis water extraction thick paste are added and ground at normal temperature, so that in-situ complexation reaction of plant organic acid and tortoise shell calcium carbonate is caused. The original components in the prescription are used as natural carriers and complexing agents, and the amorphous state of the difficult-to-dissolve components and the organic modification of inorganic calcium are realized without adding synthetic auxiliary materials. The medicine combination significantly improves the dissolution rate of psoralen and the calcium release amount in a neutral environment, and has excellent osteogenic activity.

This invention provides a fingerprint spectrum of Yishen Wufa Oral Liquid, its construction method, and its application, as well as a multi-index component detection method for Yishen Wufa Oral Liquid, relating to the field of pharmaceutical analysis and structural technology. The fingerprint spectrum of Yishen Wufa Oral Liquid constructed by this invention includes 20 characteristic peaks, including ferulic acid, 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, astragaloside A, psoralen, isopsoralen, and emodin. The chromatographic peaks are abundant and attributed to the effective components of psoralen (roasted with black sesame seeds), angelica, poria cocos, wolfberry, fleeceflower root (processed with black bean wine), achyranthes bidentata, and astragalus complanatus. This provides a more comprehensive reflection of the overall chemical composition and quality of Yishen Wufa Oral Liquid, facilitating more effective guidance for feed addition and strict standardization of production operations. It also helps improve the safety and efficacy of Yishen Wufa Oral Liquid in clinical use, possessing high application value.

The application provides application of psoralen in activating NK cells. The psoralen provided by the application is a coumarin component extracted from traditional Chinese medicine psoralea; through in-vitro cell experiment verification, the psoralen can activate NK-92 to secrete IFN-gamma, Granzyme B and Perforion, and activate surface protein NKG2D of NK-92; meanwhile, the psoralen also has the same effect on a cell group containing NK cells; through in-vivo experiment verification, the psoralen helps to improve the total amount of NK cells in the mouse spleen, inhibit tumor growth, and improve the expression amount of IFN-gamma, Granzyme B, Perforion, NKG2D, NKP46 and CD107a in a tumor infiltration environment. In summary, the small-molecule compound psoralen has good effect in activating NK cells.

The invention provides a preparation method of water-soluble psoralen traditional Chinese medicine derived carbon quantum dots, and belongs to the technical field of nano materials. According to the invention, psoralen traditional Chinese medicines are adopted as raw materials, nitrite with reducibility is introduced to improve the water solubility of the psoralen traditional Chinese medicine derived carbon quantum dots, organic solvents and surfactant molecules do not need to be adopted for modification, and nitrite is utilized to introduce nitrogen-related groups. The preparation method is simple, the operation process is simple, and the prepared psoralen traditional Chinese medicine derived carbon quantum dots also have good water solubility under high concentration, have blue-green wave band fluorescence emission, and have wide application prospects in the field of biomedicine.

This invention discloses a psoralen-loaded albumin-manganese dioxide nanoparticle formulation, its preparation method, and its applications. The nanoparticle formulation is composed of psoralen, manganese dioxide, polyacrylamide hydrochloride, and albumin, wherein the mass ratio of psoralen, manganese dioxide, polyacrylamide hydrochloride, and albumin is 5-25:2.5:2.5-12.5:0.8-6. This nanoparticle formulation significantly improves the solubility and bioavailability of psoralen, and exhibits acid-sensitive and delayed-release properties. It also demonstrates good tumor targeting and can be used as a regulator of hypoxia and tumor-associated macrophages, improving the microenvironment of triple-negative breast cancer, and thus for the preparation of drugs for treating triple-negative breast cancer.

The invention belongs to the technical field of nano materials and antibiosis, and particularly discloses a preparation method and application of bakuchiol carbon quantum dots. According to the method, bakuchiol is taken as a carbon source, ethyl acetate is taken as a solvent, the carbon quantum dots are synthesized by adopting a solvothermal method, and the optimized reaction conditions are 150 DEG C and 12 hours. The carbon quantum dot is spherical, has a particle size of 6-8 nm, is uniformly dispersed, is rich in hydroxyl, carboxyl and other groups on the surface, and has good hydrophilicity and fluorescence performance. An antibacterial experiment shows that when the concentration of the bakuchiol carbon quantum dots is gt; when the concentration is 250 [mu] g / mL, the obvious antibacterial effect on staphylococcus aureus is achieved. The method is simple in preparation process and can be applied to the fields of antibacterial materials, fluorescence detection and the like.

The invention discloses a two-step immobilization and step-by-step separation method of an RNA-protein compound, and belongs to the field of analytical chemistry. The protein is crosslinked by utilizing the characteristic that N-hydroxysuccinimide ester (NHS) structures at two ends of a DSP (Digital Signal Processor) structure react with amino groups of the protein, and the complete RPC is enriched and captured from living cells by utilizing the characteristic that 254nm crosslinked RNA and a Psoralen probe capture RNA and are compatible with M-280 magnetic beads. And respectively eluting the RNA indirect binding protein and the RNA direct binding protein which form the RNA-protein compound by utilizing the property that the TCEP breaks the disulfide bond, so as to carry out proteomics mass spectrometry analysis on the RNA binding protein in the RNA-protein compound.

This invention discloses the application of psoralen in the preparation of FFA4 receptor agonists and drugs for FFA4 receptor-related diseases. Cellular experiments show that this compound is a concentration-dependent FFA4 agonist; animal experiments show that this compound has significant efficacy in a DSS-induced colitis model, reducing DSS-induced colonic shortening, improving weight loss, and alleviating splenic swelling. This compound can be used in the preparation of FFA4 agonists and in the prevention and treatment of FFA4-related diseases such as inflammatory bowel disease.

The invention discloses hair loss preventing, hair blackening, hair growing and dandruff removing shampoo, belongs to the field of traditional Chinese medicines, and relates to the technical field of daily chemical products. The traditional Chinese medicine comprises the following raw materials: saponin, glossy privet fruit, eclipta, cacumen biotae, angelica sinensis, ligusticum wallichii, radix rehmanniae preparata, radix paeoniae alba, gastrodia elata, notopterygium root, pawpaw, semen cuscutae, divaricate saposhnikovia root, fructus psoraleae, radix bupleuri, radix polygoni multiflori preparata, folium artemisiae argyi, radix stemonae, ginger, soapberry, sasangua cake powder, elm bark, tremella fuciformis, edible salt and purified water. In the composition process, the inventor reasonably and flexibly applies the theoretical basis of traditional Chinese medicine, refers to multiple traditional Chinese medicine classifications such as Huangdi Neijing and Shennong herbal meridian, combines clinical traditional Chinese medicine diagnosis and treatment experience, and performs composition through syndrome differentiation treatment, so that the traditional Chinese medicine composition aims at preventing alopecia, blackening hair, growing hair and removing dandruff according to the traditional Chinese medicine treatment principles of tonifying liver and kidney, dispelling wind-cold-dampness, soothing liver and nourishing liver, and activating and nourishing blood. The main components of the formula are Chinese herbal medicines, damage of chemical components to the skin is reduced or even avoided as much as possible, and meanwhile the anti-alopecia, blackening, hair growing and dandruff removing effects are good.

The invention discloses a quality detection method of smoked plum granules for stopping and benefiting, and belongs to the technical field of quality detection of traditional Chinese medicines. The method comprises the following steps: identifying a characteristic spectrum; the method comprises the following specific steps: (1) respectively preparing a hyperoside reference substance solution, a coumarin reference substance solution, a lobetyolin reference substance solution, a cinnamic acid reference substance solution, a psoralen reference substance solution, an isopsoralen reference substance solution and a limonin reference substance solution; (2) preparing a test solution from the smoked plum granules; and (3) respectively carrying out liquid chromatography detection on the seven reference substance solutions and the test solution. The quality detection method disclosed by the invention is high in accuracy, strong in specificity and reliable in detection result, realizes effective control on the quality of the smoked plum granules, is suitable for modern production requirements, and has important significance on quality detection of traditional Chinese medicines and clinical medication.

The invention discloses a preparation method of a bakuchiol-loaded exosome and application of the bakuchiol-loaded exosome. The method comprises the following steps: S1, mixing supernate of a collected umbilical cord mesenchymal stem cell hUMSC culture supernate sample with Exosome Concentration Solution, oscillating and uniformly mixing through a vortex oscillator, and then standing at 4 DEG C to capture exosome particles; s2, centrifuging, discarding the supernatant, re-suspending the precipitate, centrifuging, reserving the supernatant, namely an exosome particle crude product, transferring to a purification column, and purifying to obtain purified exosome particles; and S3, mixing the exosome particles obtained in S2 with bakuchiol BAK, and carrying out drug loading treatment by using an ultrasonic cell crusher. The drug-loaded exosome can inhibit inflammation, promote collagen and angiogenesis and accelerate wound healing through subcutaneous injection near a wound, the treatment effect of the exosome is maximized, and the drug-loaded exosome has practical value in the field of skin wound healing.

The present invention discloses a quality detection method for Wumeizhili granules, belonging to the technical field of quality detection of traditional Chinese medicines. The method includes characteristic spectrum identification, and specifically comprises the following steps: (1) preparing a reference solution of hyperoside, a reference solution of coumarin, a reference solution of codonopsis pilosula, a reference solution of cinnamic acid, a reference solution of psoralen, a reference solution of isopsoralen, and a reference solution of limonin; (2) preparing a test solution using Wumeizhili granules; and (3) performing liquid chromatography detection on the seven reference solutions and the test solution. The quality detection method of the present invention has high accuracy, strong specificity, and reliable detection results, achieving effective quality control of Wumeizhili granules, meeting the needs of modern production, and having important significance for quality detection and clinical use of traditional Chinese medicines.

This invention belongs to the field of pharmaceutical technology, specifically relating to the application of psoralen in the preparation of drugs for the prevention and treatment of rheumatoid arthritis. This invention aims to address the problem of insufficient efficacy and inadequate clinical needs of existing rheumatoid arthritis prevention and treatment drugs, filling the technological gap in the pharmacological activity of psoralen against rheumatoid arthritis. Studies have confirmed that psoralen can effectively inhibit the secretion of IL-6 and IL-8 by RA-FLS cells, effectively inhibit RA-FLS cell proliferation, migration, and invasion, significantly improve joint swelling and weight loss symptoms in CIA mice, effectively regulate immune imbalance, and effectively repair joint pathological damage. It blocks the pathological processes of rheumatoid arthritis, including inflammation, immune disorders, synovial damage, and bone destruction, from multiple pathways and dimensions. Furthermore, psoralen is a natural plant-derived component. This invention provides a novel natural drug option for the treatment of rheumatoid arthritis, effectively improving the existing treatment system for the disease.