44 results about "Psoralen" patented technology

The invention discloses a pichia pastoris engineering bacterium for synthesizing bakuchiol from de novo by converting methanol as well as construction and application of the pichia pastoris engineering bacterium. A heterologous p-coumaric acid synthesis pathway is introduced to neutral sites of different chromosomes of pichia pastoris, a high-yield p-coumaric acid strain is obtained through gene knockout, overexpression or heterologous expression of tyrosine and phenylalanine synthesis pathway key genes of a shikimic acid pathway, a heterologous bakuchiol biosynthesis pathway is introduced on the basis, and a high-yield p-coumaric acid strain is obtained. After overexpression of bakuchiol synthetase, endogenous overexpression or heterologous expression of MVA pathway key genes and exogenous acetyl coenzyme A supply pathway genes, methanol concentration optimization and bacterial strain His4 gene supplementation, the yield of bakuchiol is effectively increased to 91.2 mg / L and is increased by 59.8 times compared with that of an initial bacterial strain, and the yield of bakuchiol in a 15L fermentation tank reaches 692.8 mg / L. The method has the characteristics of high conversion efficiency, low production cost, convenience in preparation, wide industrial application prospect and the like.

The application provides a method for determining components in Zhichun capsules, and simultaneously determines the contents of four components, i.e., isopsoralen, psoralen, icariin and cimicifugan, in Zhichun capsules by using high performance liquid chromatography-ultraviolet detection. By reasonably selecting chromatographic conditions and detection conditions, the method can realize rapid extraction and detection of the four components in Zhichun capsules, and has the advantages of simplicity, high sensitivity, fast analysis speed and the like, and can be used for quality control of Zhichun capsules.

The invention discloses a green method for preparing bakuchiol and application of bakuchiol in oxidation resistance. The invention provides a green method for preparing bakuchiol, aiming at the problems of low extraction rate, large loss of active ingredients, harm to health due to solvent residue, complicated extraction process and the like in the current bakuchiol extraction process, a deep eutectic solvent is used as an extraction medium, and a fructus psoraleae extract is extracted under the assistance of ultrasonic waves. The bakuchiol is further obtained through macroporous resin purification and separation, and the bakuchiol prepared by the method has remarkable antioxidant activity. According to the preparation method provided by the invention, the extraction content of the bakuchiol is remarkably increased, the problems existing in a traditional extraction process are solved, compared with a traditional organic solvent extract, the preparation method is simple and easy to operate, and the prepared bakuchiol is high in content, better in biological activity, green, safe, free of solvent residues and suitable for industrial production. The compound can be used for preparing more antioxidant drugs.

The present application relates to a hybridoma cell strain secreting psoralen and isopsoralen monoclonal antibody. The hybridoma cell strain of the present application is preserved in China General Microbiological Culture Collection Center on April 18, 2024, the preservation address is No. 1, Xibaixili, Chaoyang District, Beijing, and the preservation number is CGMCC NO. 45909. The psoralen and isopsoralen monoclonal antibody secreted by the hybridoma cell strain of the present application has good sensitivity and specificity to psoralen and isopsoralen, so that the psoralen and isopsoralen can be efficiently and rapidly detected.

This invention provides a chalcone isopentenyltransferase gene. GiPT16 This invention relates to the GiPT16 protein, amplification primer set, and applications, belonging to the field of biogenetics technology. It is based on *Glycyrrhiza inflata* (GiPT16 protein, amplification primer set, and applications). Glycyrrhiza inflata Using whole-genome sequencing data and a reverse genetics strategy, the key aromatic isopentenyltransferase GiPT16, involved in the isopentenylation modification of chalcone active ingredients, was successfully identified and functionally characterized. This enzyme was confirmed to specifically catalyze the biosynthesis of psoralen and glycyrrhizin C using DMAPP as a donor. It is the first chalcone isopentenyltransferase characterized in *Glycyrrhiza inflata*, filling a gap in the study of key enzymes in the chalcone isopentenylation metabolic pathway of this species and providing important evidence for further elucidating the molecular mechanisms of quality formation in *Glycyrrhiza inflata* medicinal materials.

The application discloses a psoralen derivative or a pharmaceutical salt thereof, and a structure general formula of the psoralen derivative is shown in the following formula: The psoralen derivative is obtained by optimizing the structure of psoralen, and the antibacterial activity test is carried out on common gram-positive bacteria such as drug-resistant staphylococcus aureus and gram-negative bacteria such as escherichia coli and klebsiella pneumoniae, and it is found that the compound or the pharmaceutical salt thereof has excellent anti-drug-resistant bacteria, anti-inflammatory and anti-tumor activities, and has good development value in preparation of anti-infection treatment drugs, anti-inflammatory drugs and anti-tumor drugs.

This invention proposes the application of psoralen as an Nrf2 agonist in drug preparation. This invention is the first to discover that psoralen is a specific Nrf2 agonist. Psoralen can directly target and bind to the Nrf2 protein, improving its stability, promoting its phosphorylation and nuclear translocation, thereby activating the Nrf2 signaling pathway, upregulating the expression of downstream antioxidant, anti-inflammatory, and anti-ferroptosis-related proteins, and achieving cell protection and tissue repair functions.

The application belongs to the technical field of cosmetics, and particularly relates to a whitening and anti-aging composition containing psoralen and vitamin C and a preparation method thereof. The whitening and anti-aging composition provided by the application is composed of scutellaria baicalensis georgi extract, white pool flower seed oil, psoralen and vitamin C, has a rapid and efficient whitening and anti-aging effect, is mild and non-irritating, and can be used for preparing skin care products with whitening and anti-aging effects. In the preparation of the scutellaria baicalensis georgi extract, a deep eutectic solvent combined with a pulsed direct current field treatment is used, which has the advantages of low cost, high extraction efficiency, simple operation, non-toxicity, good biocompatibility, biodegradability and the like. The preparation method of the whitening and anti-aging composition provided by the application is simple and easy to operate, is beneficial to industrialized production and application, and has good market application value.

The application discloses application of psoralen in preparation of a medicine for treating sepsis. As a natural product, the psoralen has high safety and good tolerance, and can intervene in a pathological process of the sepsis through various action mechanisms such as inhibition of an inflammatory reaction, regulation of an immune reaction and promotion of tissue repair. When the psoralen is applied to treatment of the sepsis, the inflammatory reaction of the sepsis can be effectively controlled, tissue damage can be reduced and the mortality rate can be lowered, so that the survival rate and the life quality of the sepsis patients can be improved.

The invention discloses a method for determining chemical components in a medicine for treating vitiligo, which relates to the technical field of medicine analysis, and is characterized in that ultra-high performance liquid chromatography-mass spectrometry is adopted to determine the content of chemical components in a Bailing tablet: psoralen, isopsoralen, astragaloside, quercetin, polysaccharide and amino acid. The determination method comprises the following steps: respectively injecting a reference solution and a test solution into a liquid chromatograph for determination to obtain a chromatogram, and calculating the content of paeoniflorin in a test sample by adopting an external standard method, chromatographic conditions of a chromatogram obtained by adopting a liquid chromatograph are as follows: octadecylsilane chemically bonded silica is taken as a filling agent. According to the method for determining the chemical components in the medicine for treating leucoderma, the established detection method is verified, and the result shows that the detection method is easy and convenient to operate, accurate in result, high in specificity and good in reproducibility.

The present invention belongs to the technical field of corn bacterial wilt prevention and control, and specifically relates to a fungicide composition for preventing and controlling corn bacterial wilt. The fungicide composition comprises a compound of psoralen and zoxamide or silthiopyrad as active ingredients. The fungicide composition, which combines psoralen with zoxamide or silthiopyrad, exhibits a co-toxicity coefficient greater than 120 against corn bacterial wilt pathogens, demonstrating a synergistic effect. Compared to single zoxamide or silthiopyrad, the control efficacy is significantly improved.

The application relates to the field of medicines, and discloses a medicine combination for preventing and treating osteoporosis syndrome and a preparation method, wherein the medicine combination is made of tortoise shell, deer horn, psoralea, drynaria and rhizoma curculiginis. A stepwise liquid-phase assisted co-grinding technology is adopted, that is, psoralen extract, deer horn coarse powder and part of tortoise shell coarse powder are ground at low temperature of 0-5 DEG C, psoralen is locked in an amorphous solid dispersion state by using the keratin network of the deer horn; then the remaining tortoise shell coarse powder and drynaria-rhizoma curculiginis water extraction thick paste are added and ground at normal temperature, so that in-situ complexation reaction of plant organic acid and tortoise shell calcium carbonate is caused. The original components in the prescription are used as natural carriers and complexing agents, and the amorphous state of the difficult-to-dissolve components and the organic modification of inorganic calcium are realized without adding synthetic auxiliary materials. The medicine combination significantly improves the dissolution rate of psoralen and the calcium release amount in a neutral environment, and has excellent osteogenic activity.

This invention provides a fingerprint spectrum of Yishen Wufa Oral Liquid, its construction method, and its application, as well as a multi-index component detection method for Yishen Wufa Oral Liquid, relating to the field of pharmaceutical analysis and structural technology. The fingerprint spectrum of Yishen Wufa Oral Liquid constructed by this invention includes 20 characteristic peaks, including ferulic acid, 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, astragaloside A, psoralen, isopsoralen, and emodin. The chromatographic peaks are abundant and attributed to the effective components of psoralen (roasted with black sesame seeds), angelica, poria cocos, wolfberry, fleeceflower root (processed with black bean wine), achyranthes bidentata, and astragalus complanatus. This provides a more comprehensive reflection of the overall chemical composition and quality of Yishen Wufa Oral Liquid, facilitating more effective guidance for feed addition and strict standardization of production operations. It also helps improve the safety and efficacy of Yishen Wufa Oral Liquid in clinical use, possessing high application value.

The application provides application of psoralen in activating NK cells. The psoralen provided by the application is a coumarin component extracted from traditional Chinese medicine psoralea; through in-vitro cell experiment verification, the psoralen can activate NK-92 to secrete IFN-gamma, Granzyme B and Perforion, and activate surface protein NKG2D of NK-92; meanwhile, the psoralen also has the same effect on a cell group containing NK cells; through in-vivo experiment verification, the psoralen helps to improve the total amount of NK cells in the mouse spleen, inhibit tumor growth, and improve the expression amount of IFN-gamma, Granzyme B, Perforion, NKG2D, NKP46 and CD107a in a tumor infiltration environment. In summary, the small-molecule compound psoralen has good effect in activating NK cells.

The invention provides a preparation method of water-soluble psoralen traditional Chinese medicine derived carbon quantum dots, and belongs to the technical field of nano materials. According to the invention, psoralen traditional Chinese medicines are adopted as raw materials, nitrite with reducibility is introduced to improve the water solubility of the psoralen traditional Chinese medicine derived carbon quantum dots, organic solvents and surfactant molecules do not need to be adopted for modification, and nitrite is utilized to introduce nitrogen-related groups. The preparation method is simple, the operation process is simple, and the prepared psoralen traditional Chinese medicine derived carbon quantum dots also have good water solubility under high concentration, have blue-green wave band fluorescence emission, and have wide application prospects in the field of biomedicine.

This invention discloses a psoralen-loaded albumin-manganese dioxide nanoparticle formulation, its preparation method, and its applications. The nanoparticle formulation is composed of psoralen, manganese dioxide, polyacrylamide hydrochloride, and albumin, wherein the mass ratio of psoralen, manganese dioxide, polyacrylamide hydrochloride, and albumin is 5-25:2.5:2.5-12.5:0.8-6. This nanoparticle formulation significantly improves the solubility and bioavailability of psoralen, and exhibits acid-sensitive and delayed-release properties. It also demonstrates good tumor targeting and can be used as a regulator of hypoxia and tumor-associated macrophages, improving the microenvironment of triple-negative breast cancer, and thus for the preparation of drugs for treating triple-negative breast cancer.

The invention belongs to the technical field of nano materials and antibiosis, and particularly discloses a preparation method and application of bakuchiol carbon quantum dots. According to the method, bakuchiol is taken as a carbon source, ethyl acetate is taken as a solvent, the carbon quantum dots are synthesized by adopting a solvothermal method, and the optimized reaction conditions are 150 DEG C and 12 hours. The carbon quantum dot is spherical, has a particle size of 6-8 nm, is uniformly dispersed, is rich in hydroxyl, carboxyl and other groups on the surface, and has good hydrophilicity and fluorescence performance. An antibacterial experiment shows that when the concentration of the bakuchiol carbon quantum dots is gt; when the concentration is 250 [mu] g / mL, the obvious antibacterial effect on staphylococcus aureus is achieved. The method is simple in preparation process and can be applied to the fields of antibacterial materials, fluorescence detection and the like.

The invention discloses a two-step immobilization and step-by-step separation method of an RNA-protein compound, and belongs to the field of analytical chemistry. The protein is crosslinked by utilizing the characteristic that N-hydroxysuccinimide ester (NHS) structures at two ends of a DSP (Digital Signal Processor) structure react with amino groups of the protein, and the complete RPC is enriched and captured from living cells by utilizing the characteristic that 254nm crosslinked RNA and a Psoralen probe capture RNA and are compatible with M-280 magnetic beads. And respectively eluting the RNA indirect binding protein and the RNA direct binding protein which form the RNA-protein compound by utilizing the property that the TCEP breaks the disulfide bond, so as to carry out proteomics mass spectrometry analysis on the RNA binding protein in the RNA-protein compound.

This invention discloses the application of psoralen in the preparation of FFA4 receptor agonists and drugs for FFA4 receptor-related diseases. Cellular experiments show that this compound is a concentration-dependent FFA4 agonist; animal experiments show that this compound has significant efficacy in a DSS-induced colitis model, reducing DSS-induced colonic shortening, improving weight loss, and alleviating splenic swelling. This compound can be used in the preparation of FFA4 agonists and in the prevention and treatment of FFA4-related diseases such as inflammatory bowel disease.

The present invention discloses a quality detection method for Wumeizhili granules, belonging to the technical field of quality detection of traditional Chinese medicines. The method includes characteristic spectrum identification, and specifically comprises the following steps: (1) preparing a reference solution of hyperoside, a reference solution of coumarin, a reference solution of codonopsis pilosula, a reference solution of cinnamic acid, a reference solution of psoralen, a reference solution of isopsoralen, and a reference solution of limonin; (2) preparing a test solution using Wumeizhili granules; and (3) performing liquid chromatography detection on the seven reference solutions and the test solution. The quality detection method of the present invention has high accuracy, strong specificity, and reliable detection results, achieving effective quality control of Wumeizhili granules, meeting the needs of modern production, and having important significance for quality detection and clinical use of traditional Chinese medicines.

This invention belongs to the field of pharmaceutical technology, specifically relating to the application of psoralen in the preparation of drugs for the prevention and treatment of rheumatoid arthritis. This invention aims to address the problem of insufficient efficacy and inadequate clinical needs of existing rheumatoid arthritis prevention and treatment drugs, filling the technological gap in the pharmacological activity of psoralen against rheumatoid arthritis. Studies have confirmed that psoralen can effectively inhibit the secretion of IL-6 and IL-8 by RA-FLS cells, effectively inhibit RA-FLS cell proliferation, migration, and invasion, significantly improve joint swelling and weight loss symptoms in CIA mice, effectively regulate immune imbalance, and effectively repair joint pathological damage. It blocks the pathological processes of rheumatoid arthritis, including inflammation, immune disorders, synovial damage, and bone destruction, from multiple pathways and dimensions. Furthermore, psoralen is a natural plant-derived component. This invention provides a novel natural drug option for the treatment of rheumatoid arthritis, effectively improving the existing treatment system for the disease.

The invention relates to the technical field of probiotics, in particular to pediococcus acidilactici NH3 for treating male prostate and improving sexual function as well as a culture method and application of the pediococcus acidilactici NH3. The strain is preserved in Guangdong Microbial Culture Collection Center on April 18, 2025, and the preservation number is GDMCC No: 66163. The strain is a probiotic strain approved by the National Health Commission. The strain has the effects of regulating intestinal flora balance and promoting digestion and absorption of a human body, and also has the effects of treating male prostate and improving the sexual function. 5 billion strains and 50 milligrams of metabolites are given every day, so that the endophytic essential quantity of astragaloside, spinosin and psoralen can be continuously provided in the intestinal reproduction and growth process, the male prostate can be treated, the sexual function can be improved, and the body health can be kept.

The present application relates to the field of traditional Chinese medicine, in particular to a composition with anti-melanin production activity and a preparation method thereof. The composition is composed of psoralen coumarin component and euryale seed extract. The psoralen is subjected to water vapor distillation, alkali dissolution and acid precipitation, and frozen centrifugation to obtain the psoralen coumarin component. The euryale seed extract is prepared by water extraction, alcohol precipitation and macroporous resin purification using euryale seed as raw material. The psoralen coumarin component is prepared into a solid dispersion by solvent evaporation method using the euryale seed extract as a dispersion medium to obtain the composition. The inhibitory effect of the composition on melanin production can reach 58-65% when the concentration is 100 μg / mL. The process for extracting the psoralen coumarin component has strong applicability, and the solid dispersion prepared with the euryale seed extract not only plays a synergistic effect, but also improves the solubility of the effective component. It provides a natural and safe product for the clinical treatment of related skin diseases and the development of whitening skin care products.

The present application belongs to the technical field of compound synthesis, and particularly relates to a non-allergenic bakuchiol derivative, a preparation method thereof and application. The present application obtains a bakuchiol derivative with a novel structure through side chain modification. The obtained bakuchiol derivative not only maintains the whitening, antioxidant, firming and anti-wrinkle activities of bakuchiol, but also significantly improves the oxidative stability, biological safety and reduces the risk of sensitization. Thanks to the significant improvement in safety, the derivative can be applied at a higher concentration, thereby producing more excellent activity effect. In addition, the synthesis process of the derivative is simple and efficient, and the derivative has a wide application prospect in the fields of whitening, antioxidant, anti-aging and sensitive skin care.

This invention discloses the application of psoralen chalcone in the preparation of FFA4 receptor agonists and drugs for FFA4 receptor-related diseases. Cellular experiments show that this compound is a concentration-dependent FFA4 agonist; animal experiments show that this compound has significant efficacy in a DSS-induced colitis model, reducing DSS-induced colonic shortening, improving weight loss, and alleviating splenic swelling. This compound can be used in the preparation of FFA4 agonists and in the prevention and treatment of FFA4-related diseases such as inflammatory bowel disease, non-alcoholic fatty liver disease, and osteoporosis.

The invention relates to the technical field of biological medicines, in particular to a psoralen derivative and application thereof in preparation of a medicine for treating rheumatoid arthritis. According to the derivative, through an ester prodrug strategy, psoralen C8 / C5 hydroxyl and a non-steroidal anti-inflammatory drug are coupled to obtain a double-pharmacophore molecule. According to the invention, the problems of poor lipid solubility and high hepatotoxicity of psoralen are solved through an ester prodrug design strategy, a high-efficiency and low-toxicity novel drug candidate is provided for RA treatment, and the prepared psoralen derivative can effectively treat rheumatoid arthritis and complications thereof, and can significantly improve arthropathy caused by RA.

The application relates to the technical field of bacteriostatic materials, and particularly discloses a kind of anti-penicillium film based on psoralea carbon quantum dots and a preparation method and application thereof.The anti-penicillium film comprises a biodegradable film-forming substrate and a photosensitizer, the photosensitizer comprises psoralea carbon quantum dots, the psoralea carbon quantum dots take psoralea as a carbon source, the particle size of the psoralea carbon quantum dots is 1-10 nm, and the anti-penicillium film is applied to fruit and vegetable preservation.The psoralea carbon quantum dots synthesized by taking traditional Chinese medicinal material psoralea as the only carbon source are used as the photosensitizer, and the anti-penicillium film prepared by the photosensitizer has the functions of active antibiosis and physical blocking, can efficiently generate a large number of active oxygen under blue light irradiation, can realize precise and physical killing of penicillium, effectively prolongs the shelf life of fruits and vegetables, and reduces postharvest loss of fruits and vegetables.

The application provides a compound composition of psoralen and matrine, a fungicide and application; the mass ratio of psoralen to matrine is (4-1):(1-4). The compound of psoralen and natural product matrine can not only prevent and treat rice blast, but also can be used for preventing and treating rice sheath blight. The binary compound fungicide has remarkable synergistic effect on preventing and treating rice blast and rice sheath blight. The effective components of the compound fungicide are all plant source natural products, and the compound fungicide has the characteristics of safety to non-target organisms and environmental friendliness, and therefore, the compound fungicide has wide application prospect as a new generation of plant source fungicide in green pest control.