27 results about "Psoralen" patented technology

This invention provides a chalcone isopentenyltransferase gene. GiPT16 This invention relates to the GiPT16 protein, amplification primer set, and applications, belonging to the field of biogenetics technology. It is based on *Glycyrrhiza inflata* (GiPT16 protein, amplification primer set, and applications). Glycyrrhiza inflata Using whole-genome sequencing data and a reverse genetics strategy, the key aromatic isopentenyltransferase GiPT16, involved in the isopentenylation modification of chalcone active ingredients, was successfully identified and functionally characterized. This enzyme was confirmed to specifically catalyze the biosynthesis of psoralen and glycyrrhizin C using DMAPP as a donor. It is the first chalcone isopentenyltransferase characterized in *Glycyrrhiza inflata*, filling a gap in the study of key enzymes in the chalcone isopentenylation metabolic pathway of this species and providing important evidence for further elucidating the molecular mechanisms of quality formation in *Glycyrrhiza inflata* medicinal materials.

This invention proposes the application of psoralen as an Nrf2 agonist in drug preparation. This invention is the first to discover that psoralen is a specific Nrf2 agonist. Psoralen can directly target and bind to the Nrf2 protein, improving its stability, promoting its phosphorylation and nuclear translocation, thereby activating the Nrf2 signaling pathway, upregulating the expression of downstream antioxidant, anti-inflammatory, and anti-ferroptosis-related proteins, and achieving cell protection and tissue repair functions.

The application belongs to the technical field of cosmetics, and particularly relates to a whitening and anti-aging composition containing psoralen and vitamin C and a preparation method thereof. The whitening and anti-aging composition provided by the application is composed of scutellaria baicalensis georgi extract, white pool flower seed oil, psoralen and vitamin C, has a rapid and efficient whitening and anti-aging effect, is mild and non-irritating, and can be used for preparing skin care products with whitening and anti-aging effects. In the preparation of the scutellaria baicalensis georgi extract, a deep eutectic solvent combined with a pulsed direct current field treatment is used, which has the advantages of low cost, high extraction efficiency, simple operation, non-toxicity, good biocompatibility, biodegradability and the like. The preparation method of the whitening and anti-aging composition provided by the application is simple and easy to operate, is beneficial to industrialized production and application, and has good market application value.

The application relates to the field of medicines, and discloses a medicine combination for preventing and treating osteoporosis syndrome and a preparation method, wherein the medicine combination is made of tortoise shell, deer horn, psoralea, drynaria and rhizoma curculiginis. A stepwise liquid-phase assisted co-grinding technology is adopted, that is, psoralen extract, deer horn coarse powder and part of tortoise shell coarse powder are ground at low temperature of 0-5 DEG C, psoralen is locked in an amorphous solid dispersion state by using the keratin network of the deer horn; then the remaining tortoise shell coarse powder and drynaria-rhizoma curculiginis water extraction thick paste are added and ground at normal temperature, so that in-situ complexation reaction of plant organic acid and tortoise shell calcium carbonate is caused. The original components in the prescription are used as natural carriers and complexing agents, and the amorphous state of the difficult-to-dissolve components and the organic modification of inorganic calcium are realized without adding synthetic auxiliary materials. The medicine combination significantly improves the dissolution rate of psoralen and the calcium release amount in a neutral environment, and has excellent osteogenic activity.

This invention provides a fingerprint spectrum of Yishen Wufa Oral Liquid, its construction method, and its application, as well as a multi-index component detection method for Yishen Wufa Oral Liquid, relating to the field of pharmaceutical analysis and structural technology. The fingerprint spectrum of Yishen Wufa Oral Liquid constructed by this invention includes 20 characteristic peaks, including ferulic acid, 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, astragaloside A, psoralen, isopsoralen, and emodin. The chromatographic peaks are abundant and attributed to the effective components of psoralen (roasted with black sesame seeds), angelica, poria cocos, wolfberry, fleeceflower root (processed with black bean wine), achyranthes bidentata, and astragalus complanatus. This provides a more comprehensive reflection of the overall chemical composition and quality of Yishen Wufa Oral Liquid, facilitating more effective guidance for feed addition and strict standardization of production operations. It also helps improve the safety and efficacy of Yishen Wufa Oral Liquid in clinical use, possessing high application value.

The application provides application of psoralen in activating NK cells. The psoralen provided by the application is a coumarin component extracted from traditional Chinese medicine psoralea; through in-vitro cell experiment verification, the psoralen can activate NK-92 to secrete IFN-gamma, Granzyme B and Perforion, and activate surface protein NKG2D of NK-92; meanwhile, the psoralen also has the same effect on a cell group containing NK cells; through in-vivo experiment verification, the psoralen helps to improve the total amount of NK cells in the mouse spleen, inhibit tumor growth, and improve the expression amount of IFN-gamma, Granzyme B, Perforion, NKG2D, NKP46 and CD107a in a tumor infiltration environment. In summary, the small-molecule compound psoralen has good effect in activating NK cells.

This invention discloses a psoralen-loaded albumin-manganese dioxide nanoparticle formulation, its preparation method, and its applications. The nanoparticle formulation is composed of psoralen, manganese dioxide, polyacrylamide hydrochloride, and albumin, wherein the mass ratio of psoralen, manganese dioxide, polyacrylamide hydrochloride, and albumin is 5-25:2.5:2.5-12.5:0.8-6. This nanoparticle formulation significantly improves the solubility and bioavailability of psoralen, and exhibits acid-sensitive and delayed-release properties. It also demonstrates good tumor targeting and can be used as a regulator of hypoxia and tumor-associated macrophages, improving the microenvironment of triple-negative breast cancer, and thus for the preparation of drugs for treating triple-negative breast cancer.

The invention belongs to the technical field of nano materials and antibiosis, and particularly discloses a preparation method and application of bakuchiol carbon quantum dots. According to the method, bakuchiol is taken as a carbon source, ethyl acetate is taken as a solvent, the carbon quantum dots are synthesized by adopting a solvothermal method, and the optimized reaction conditions are 150 DEG C and 12 hours. The carbon quantum dot is spherical, has a particle size of 6-8 nm, is uniformly dispersed, is rich in hydroxyl, carboxyl and other groups on the surface, and has good hydrophilicity and fluorescence performance. An antibacterial experiment shows that when the concentration of the bakuchiol carbon quantum dots is gt; when the concentration is 250 [mu] g / mL, the obvious antibacterial effect on staphylococcus aureus is achieved. The method is simple in preparation process and can be applied to the fields of antibacterial materials, fluorescence detection and the like.

The invention discloses a two-step immobilization and step-by-step separation method of an RNA-protein compound, and belongs to the field of analytical chemistry. The protein is crosslinked by utilizing the characteristic that N-hydroxysuccinimide ester (NHS) structures at two ends of a DSP (Digital Signal Processor) structure react with amino groups of the protein, and the complete RPC is enriched and captured from living cells by utilizing the characteristic that 254nm crosslinked RNA and a Psoralen probe capture RNA and are compatible with M-280 magnetic beads. And respectively eluting the RNA indirect binding protein and the RNA direct binding protein which form the RNA-protein compound by utilizing the property that the TCEP breaks the disulfide bond, so as to carry out proteomics mass spectrometry analysis on the RNA binding protein in the RNA-protein compound.

This invention discloses the application of psoralen in the preparation of FFA4 receptor agonists and drugs for FFA4 receptor-related diseases. Cellular experiments show that this compound is a concentration-dependent FFA4 agonist; animal experiments show that this compound has significant efficacy in a DSS-induced colitis model, reducing DSS-induced colonic shortening, improving weight loss, and alleviating splenic swelling. This compound can be used in the preparation of FFA4 agonists and in the prevention and treatment of FFA4-related diseases such as inflammatory bowel disease.

This invention belongs to the field of pharmaceutical technology, specifically relating to the application of psoralen in the preparation of drugs for the prevention and treatment of rheumatoid arthritis. This invention aims to address the problem of insufficient efficacy and inadequate clinical needs of existing rheumatoid arthritis prevention and treatment drugs, filling the technological gap in the pharmacological activity of psoralen against rheumatoid arthritis. Studies have confirmed that psoralen can effectively inhibit the secretion of IL-6 and IL-8 by RA-FLS cells, effectively inhibit RA-FLS cell proliferation, migration, and invasion, significantly improve joint swelling and weight loss symptoms in CIA mice, effectively regulate immune imbalance, and effectively repair joint pathological damage. It blocks the pathological processes of rheumatoid arthritis, including inflammation, immune disorders, synovial damage, and bone destruction, from multiple pathways and dimensions. Furthermore, psoralen is a natural plant-derived component. This invention provides a novel natural drug option for the treatment of rheumatoid arthritis, effectively improving the existing treatment system for the disease.

The invention relates to the technical field of probiotics, in particular to pediococcus acidilactici NH3 for treating male prostate and improving sexual function as well as a culture method and application of the pediococcus acidilactici NH3. The strain is preserved in Guangdong Microbial Culture Collection Center on April 18, 2025, and the preservation number is GDMCC No: 66163. The strain is a probiotic strain approved by the National Health Commission. The strain has the effects of regulating intestinal flora balance and promoting digestion and absorption of a human body, and also has the effects of treating male prostate and improving the sexual function. 5 billion strains and 50 milligrams of metabolites are given every day, so that the endophytic essential quantity of astragaloside, spinosin and psoralen can be continuously provided in the intestinal reproduction and growth process, the male prostate can be treated, the sexual function can be improved, and the body health can be kept.

The present application relates to the field of traditional Chinese medicine, in particular to a composition with anti-melanin production activity and a preparation method thereof. The composition is composed of psoralen coumarin component and euryale seed extract. The psoralen is subjected to water vapor distillation, alkali dissolution and acid precipitation, and frozen centrifugation to obtain the psoralen coumarin component. The euryale seed extract is prepared by water extraction, alcohol precipitation and macroporous resin purification using euryale seed as raw material. The psoralen coumarin component is prepared into a solid dispersion by solvent evaporation method using the euryale seed extract as a dispersion medium to obtain the composition. The inhibitory effect of the composition on melanin production can reach 58-65% when the concentration is 100 μg / mL. The process for extracting the psoralen coumarin component has strong applicability, and the solid dispersion prepared with the euryale seed extract not only plays a synergistic effect, but also improves the solubility of the effective component. It provides a natural and safe product for the clinical treatment of related skin diseases and the development of whitening skin care products.

This invention discloses the application of psoralen chalcone in the preparation of FFA4 receptor agonists and drugs for FFA4 receptor-related diseases. Cellular experiments show that this compound is a concentration-dependent FFA4 agonist; animal experiments show that this compound has significant efficacy in a DSS-induced colitis model, reducing DSS-induced colonic shortening, improving weight loss, and alleviating splenic swelling. This compound can be used in the preparation of FFA4 agonists and in the prevention and treatment of FFA4-related diseases such as inflammatory bowel disease, non-alcoholic fatty liver disease, and osteoporosis.

The application relates to the technical field of bacteriostatic materials, and particularly discloses a kind of anti-penicillium film based on psoralea carbon quantum dots and a preparation method and application thereof.The anti-penicillium film comprises a biodegradable film-forming substrate and a photosensitizer, the photosensitizer comprises psoralea carbon quantum dots, the psoralea carbon quantum dots take psoralea as a carbon source, the particle size of the psoralea carbon quantum dots is 1-10 nm, and the anti-penicillium film is applied to fruit and vegetable preservation.The psoralea carbon quantum dots synthesized by taking traditional Chinese medicinal material psoralea as the only carbon source are used as the photosensitizer, and the anti-penicillium film prepared by the photosensitizer has the functions of active antibiosis and physical blocking, can efficiently generate a large number of active oxygen under blue light irradiation, can realize precise and physical killing of penicillium, effectively prolongs the shelf life of fruits and vegetables, and reduces postharvest loss of fruits and vegetables.

The application provides a compound composition of psoralen and matrine, a fungicide and application; the mass ratio of psoralen to matrine is (4-1):(1-4). The compound of psoralen and natural product matrine can not only prevent and treat rice blast, but also can be used for preventing and treating rice sheath blight. The binary compound fungicide has remarkable synergistic effect on preventing and treating rice blast and rice sheath blight. The effective components of the compound fungicide are all plant source natural products, and the compound fungicide has the characteristics of safety to non-target organisms and environmental friendliness, and therefore, the compound fungicide has wide application prospect as a new generation of plant source fungicide in green pest control.

The invention discloses a construction method of a standard decoction fingerprint spectrum of radix fici simplicissimae decoction pieces, and relates to the technical field of traditional Chinese medicines. The construction method comprises the following steps: adding psoralen and bergapten into a methanol aqueous solution with the volume fraction of 50% to prepare a reference substance solution; the method comprises the following steps: respectively carrying out water extraction treatment on batches of radix fici simplicissimae to obtain different batches of standard decoction, respectively adding methanol into the different batches of standard decoction until the volume fraction of methanol is 50%, and then centrifuging to take supernatant to obtain different batches of test solution; and respectively performing high performance liquid chromatography detection on the different batches of test sample solutions to obtain fingerprint spectrums, and calibrating characteristic peaks by using the reference substance solution. By utilizing the construction method, the fingerprint spectrum of the radix fici simplicissimae decoction piece standard decoction can be accurately constructed, so that reference and basis are provided for overall quality control and evaluation of the radix fici simplicissimae decoction piece standard decoction and formula granules.

The invention belongs to the technical field of biological medicines, and particularly relates to application of psoralen in preparation of a medicine for treating skeletal muscle atrophy. The skeletal muscle atrophy is caused by chronic heart failure. Animal experiments prove that the psoralen can increase the skeletal muscle mass of mice with chronic heart failure, increase the exercise tolerance and limb tension of the mice, relieve the inflammatory response of skeletal muscle tissues and improve the pyroptosis level of the skeletal muscle tissues, and has a very good curative effect on skeletal muscle atrophy caused by chronic heart failure; moreover, the traditional Chinese medicine composition has no influence on the liver of a mouse, and a brand new solution is provided for clinically preventing and treating skeletal muscle atrophy caused by chronic heart failure.

A kind of overall quality control method of compound eucommia ulmacea waist capsule, including: psoralen and isopsoralen content determination: with hydrochloric acid-methanol solution as extraction solvent, heating reflux extraction, with methanol-phosphoric acid aqueous solution as mobile phase isocratic elution;Macon glycoside, paeoniflorin and paeonol glycoside content determination: with 50% ethanol ultrasonic extraction, with acetonitrile-0.1% phosphoric acid solution as mobile phase gradient elution;HPLC fingerprint is established: 21 common peaks are calibrated, 6 characteristic peaks are identified, and the similarity is not less than 0.992;Thin layer chromatography identification: establish white peony root and cornus officinalis synchronous identification, ephedra identification and radix rehmanniae praeparata identification method.The present application is based on the identification of 92 kinds of compounds by LC-MS technology, and a multi-dimensional quality control system combining multi-component quantitative, fingerprint overall evaluation and exclusive identification is constructed, which overcomes the shortcomings of single existing standard index and lack of overall evaluation, and can comprehensively and accurately control the quality of compound eucommia ulmacea waist capsule.

The invention discloses a preparation method of a detoxification and leucorrhea arresting mixture, the mixture prepared by the method, and a quality control method and application of the mixture. The mixture is characterized in that in every 1mL of the mixture, the content of psoralen (C11H6O3) is not less than 14.89 [mu] g, and the content of astilbin (C21H22O11) is not less than 0.54 mg. The preparation method comprises the integrated steps of extracting medicinal materials, concentrating an extracting solution until the relative density is 1.06-1.10 at the temperature of 60 DEG C, standing and purifying, and adding stevioside and sodium benzoate in a specific proportion to prepare liquid and form. The invention also establishes a comprehensive quality control system covering thin-layer chromatography identification of multiple medicines including ovateleaf holly bark, pericarpium citri reticulatae, liquorice and plantain herb and content determination of the two-index components. In-vitro and clinical evidences show that the mixture has antibacterial and anti-inflammatory effects and has a good application prospect in treating gynecological diseases related to human papilloma virus (HPV) infection.

The invention is applicable to the technical field of bakuchiol preparation, and provides a method for preparing high-purity bakuchiol by combining a deep eutectic solvent with high-speed counter-current chromatography, which comprises the following steps: mixing choline chloride with a hydrogen bond acceptor to obtain DES; mixing the fructus psoraleae powder with DES, extracting the mixture in a microwave extraction device, and carrying out suction filtration to collect filtrate; adding an extraction solvent into the filtrate, oscillating, extracting, layering, collecting an upper-layer organic phase, and concentrating under reduced pressure until the upper-layer organic phase is dry, so as to obtain a crude extract; preparing an n-hexane-ethyl acetate-methanol-water solvent system, dissolving the fructus psoraleae crude extract by using a mixed solvent of upper and lower phases, pumping into a mobile phase by adopting a high-speed counter-current chromatograph, and collecting fractions; and concentrating under reduced pressure and drying in vacuum. The DES extraction technology and the HSCCC purification technology are combined and applied to preparation of the bakuchiol, a brand-new and green technical path is formed, the problem that the bakuchiol is difficult to separate from structural analogues is solved, extremely high purity can be achieved in one step, DES and HSCCC solvents can be recycled, and the production cost and environmental pollution are reduced.

The application belongs to the field of traditional Chinese medicines, and provides a method for constructing a kidney-strengthening tablet fingerprint spectrum, which comprises: detecting test sample solution and reference material solution by HPLC, wherein the chromatographic conditions are as follows: using octadecylsilane bonded silica gel as a filler; using acetonitrile as mobile phase A and 0.05% formic acid water as mobile phase B, and identifying 13 chromatographic peaks, which are 5-hydroxymethyl furfural, morroniside, motherwort alkaloid hydrochloride, loganin, pinoresinol di-glucoside, paeoniflorin, salvianolic acid B, psoralen, isopsoralen, paeonol, cryptotanshinone, tanshinone I and tanshinone IIA, so that the quality of the kidney-strengthening tablet can be comprehensively and accurately characterized. Meanwhile, the detection method in the application is stable and feasible. The characteristic spectrum established by the application can comprehensively reflect the complex chemical components and relative proportions of the kidney-strengthening tablet, and can effectively characterize the quality of the medicine.

The invention provides a chalcone isopentenyl transferase gene GiPT16, a GiPT16 protein, an amplification primer group and application, and belongs to the technical field of biological genes. The key aromatic isopentenyl transferase GiPT16, which participates in isopentenylation modification of chalcone active ingredients, is successfully mined and functionally identified by adopting a reverse genetics strategy on the basis of whole genome sequencing data of Glycyrrhiza inflata (Glycyrrhiza inflata). The chalcone isopentenyl transferase is proved to be capable of specifically catalyzing biosynthesis of psoralen B and licochalcone C by taking DMAPP as a donor, is the first represented chalcone isopentenyl transferase in glycyrrhiza inflate, fills the blank of key enzyme research of the species in a chalcone isopentenyl metabolic pathway, and has a wide application prospect. An important basis is provided for deeply analyzing a molecular mechanism formed by the quality of the glycyrrhiza inflate medicinal material.

This invention discloses a shower gel with antibacterial function, specifically relating to the technical field of shower gels. This shower gel aims to solve the problems of existing shower gels being unsuitable for damaged skin and hindering wound healing with long-term use. The shower gel of this invention contains the following components by weight percentage: 0.05%-0.2% hinoki alcohol; 1.0%-5.0% psoralen extract; 1.0%-8.0% Tween 20; 3.0%-10.0% propylene glycol; 0.5%-3.0% phospholipids; 0.01%-0.15% benzalkonium chloride; appropriate amount of pH adjuster; and the balance being purified water. This invention achieves a gentle and non-irritating cleansing effect through the selection and proportioning of specific components, while also exhibiting a significant inhibitory effect on common pathogenic bacteria. It is particularly suitable for the cleansing and care of skin around postoperative wounds and sensitive skin. This shower gel does not contain alcohol, fragrance, pigments, or irritating surfactants, avoiding secondary damage to damaged skin caused by traditional products. It has good system stability and biocompatibility, providing a safe and effective whole-body cleansing solution for postoperative patients.

The present application belongs to the field of biotechnology. More particularly, it relates to a hair loss prevention serum and a preparation method thereof. The hair loss prevention serum comprises the following components in mass fraction: a hair loss prevention composition 1-3%, a humectant 2-5%, a solubilizer 1-5%, a thickening agent 0.1-1%, a hair conditioner 1-3%, a soothing agent 1-3%, a pH regulator 0.1-1%, and a solvent in the remaining amount; and the hair loss prevention composition is composed of psoralen and 6-pyrrolidinyl-2,4-diaminopyrimidine-3-oxide in a weight ratio of 1:0.1-1. The hair loss prevention serum is specially designed for patients with androgenetic alopecia, and is mainly effective in preventing hair loss and improving hair quality. The core components are psoralen and dianinopyrimidine oxide, supplemented by South American amaranth root extract, glycerol, and cocoyl glutamic acid, and the serum has the characteristics of strong hair loss prevention and no irritation.

The invention provides a screening method for active ingredients of a Gushukang preparation based on a cell membrane chromatography technology, and belongs to the technical field of traditional Chinese medicine substance screening. The method comprises the following steps: S1, preparing an osteoblast cell membrane chromatographic column, S2, treating a Gushukang extract, S3, performing HPLC-MS / MS analysis, and S4, verifying binding activity. The UMR-106 osteoblast cell membrane is used as a stationary phase, so that the screening process can be directly combined and recognized based on the native conformation of a cell membrane receptor, and cell membrane protein with higher activity is obtained through low-temperature PBS washing, ice-bath ultrasonic cracking and differential centrifugal extraction; four key active components, including icariin, psoralen, pinoresinol diglucoside and ferulic acid, which are specifically bound with osteogenic cell membranes in the Gushukang capsules / granules are successfully screened by adopting a cell membrane chromatography technology, and the screening efficiency and accuracy are remarkably improved.