70 results about "Periodontal ligament stem cells" patented technology

The invention generally relates to postnatal periodontal ligament stem cells and methods for their use. More specifically, the invention relates in one aspect to postnatal periodontal ligament multipotent stem cells, use of the cells to generate periodontium, differentiation of the cells and methods of tissue cryopreservation.

A method for reconstructing a functional tooth by using a carrier loaded with a plurality of mesenchymal stem cells including at least one of a stem cell derived from Apical Papilla (SCAP), Periodontal Ligament Stem Cells (PDLSC), and Dental Pulp Stem Cells (DPSC). The mesenchymal stem cell loaded carrier is planted into a site within a subject's oral cavity to form a bio-root upon which a crown may be affixed, The mesenchymal stem cells will develop new periodontal tissues to stabilize the bio-root. Methods disclosed herein are particularly beneficial for subjects lacking good bone structures for conventional crown treatments.

The invention relates to a preparation method of a specific extracellular matrix ECM of periodontium. The preparation method comprises the following steps: 1) separating and culturing a human periodontal ligament cell; and 2) preparing the specific extracellular matrix ECM of periodontium; applying the specific extracellular matrix ECM of periodontium and applying the specific extracellular matrix ECM of periodontium as a human periodontal ligament stem cell in in-vitro amplification; and promoting directional differentiation of the ECM. The preparation method is simple and can remarkably promote in-vitro amplification and directional differentiation of the periodontal ligament stem cell.

The invention discloses stem cells for specifically expressing a programmed death receptor 1 (PD-1), and particularly relates to the stem cells from an organic cavity. The stem cells comprise but notlimited to dental pulp stem cells, gingival mesenchymal steam cells (GMSCs), periodontal ligament stem cells (PDLSCs), stem cells of apical papilla (SCAPs) and dental follicle stem cells (DFSCs) or any combination thereof, and preferably comprise stem cells from human exfoliated deciduous teeth (SHED) and/or dental pulp mesenchymal stem cells for permanent teeth (DPSC); the stem cells also includemesenchymal stem cells from other tissues, which do not express the PD-1 at first and express the PD-1 after being modified by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), suchas PD-1 plus bone mesenchymal stem cells (BMMSC) modified by the CRISPR. The invention further discloses an identifying and separating method for the stem cells for specifically expressing the PD-1,a method for preparing the PD-1 plus mesenchymal stem cells modified by the CRISPR as well as application of the mesenchymal stem cells for expressing the PD-1, disclosed by the invention, in tissue regeneration, pain relief and treatment of chronic pain and a series of diseases.

The invention discloses a two-layer composite periodontal defect repair material based on photo-crosslinking hydrogel and a preparing method of the material. The periodontal defect repair material isprepared with biomacromolecules GelMa and synthetic macromolecules PEGDMA which both have photo-crosslinking features. The two-layer composite hydrogel material can integrate the advantages of a GelMAfiber hydrogel layer and the advantages of a PEGDMA hydrogel layer, so that the two-layer composite hydrogel material has the shapes of three-dimensional fibers and communicated pores, good biocompatibility, high hydrophilicity and excellent mechanical performance and stability. The GelMA fiber hydrogel layer can regulate and control differentiation of periodontal ligament stem cells towards osteoblasts through fiber size on the condition that no growth factor is added, and tissue regeneration of the alveolar bone is effectively promoted; the PEGDMA hydrogel layer can effectively stop soft tissue (gingival tissue) from invading a defect area, and an effective space is provided for the tissue regeneration of the alveolar bone; in the meanwhile, the transportation of nutrient substances isnot influenced while the stopping effect is achieved.

The invention relates to a method for extracting and purifying neutral pseudo-ginseng polysaccharide, a research and an application for pharmacological activity for promoting cell proliferation. The pseudo-ginseng waste residue of the notoginsenoside extracted in the production process is taken as a raw material, a water extract and alcohol precipitation method is adopted for acquiring crude pseudo-ginseng polysaccharide and the DEAE Sepharose Fast Flow anion exchange chromatography is adopted for purifying the crude pseudo-ginseng polysaccharide so as to acquire five components: neutral pseudo-ginseng polysaccharide PNPS I and acidic pseudo-ginseng polysaccharide PNPS II, PNPS III, PNPS IV and PNPS V five samples. The research on the influence of the pseudo-ginseng polysaccharide on human periodontal ligament stem cell, mice osteoblast and human skin epidermis cell in vitro proliferation proves that the neutral pseudo-ginseng polysaccharide PNPS I is effective in boosting the periodontal ligament stem cell, mice osteoblast and human skin epidermis cell in vitro proliferation and the neutral pseudo-ginseng polysaccharide PNPS I can be used as an important drug intermediate for developing a new drug and a natural raw material for a functional healthcare food and also can be used as an active raw material of the household chemicals.

The invention discloses an effect of LncRNA-TUG1 on regulating and controlling PDLSCs osteogenic differentiation and tissue regeneration. Based upon in vivo and in vitro experiments of researching the effect of the LncRNA-TUG1 on regulating and controlling PDLSCs osteogenic differentiation and tissue regeneration, the LncRNA-TUG1 plays an important role in regulating and controlling the osteogenic differentiation and tissue regeneration of periodontal ligament stem cells; and the LncRNA-TUG1, as a potential target in tissue regeneration therapy, can promote the development of novel LncRNA-TUG1 related medicines or factors, and the LncRNA-TUG1 can be finally applied to the field of regenerative medicine, so that real precision medicine in the clinical field can be achieved.

The invention belongs to the field of cell cryopreservation and particularly relates to a periodontal ligament stem cell cryoprotectant and a cryopreservation method thereof. The cryoprotectant disclosed by the invention is prepared from glycerinum, mesenchymal steam cell serum-free medium and human serum albumin. The cryoprotectant disclosed by the invention can effectively solve the technical defects that an existing cryoprotectant has low use effect and a survival rate of post-resuscitation periodontal ligament stem cells is low. The periodontal ligament stem cell cryoprotectant disclosed by the invention can keep activity of cryopreserved cells; furthermore, the survival rate of the post-resuscitation periodontal ligament stem cells is obviously improved, and expression of surface markers of the stem cells and differentiation potential of the stem cells are not affected.

The disclose belongs to the field of osteogenic differentiation of periodontal ligament stem cells, and particularly relates to an application of circRNA PRKD3 to osteogenic differentiation of periodontal ligament stem cells. In order to define the osteogenic differentiation mechanism of the periodontal ligament stem cells, the inventor firstly researches and inspires that specific lncRNA and circRNA are possibly used as ceRNA to adjust the osteogenic differentiation of the periodontal ligament stem cells and influence periodontal regeneration. In order to further confirm the relationship between the circRNA and the osteogenic differentiation of the periodontal ligament stem cells, circRNA pertinent to miRNA-21 is forecast by a bioinformatics method, the situation that circRNA PRKD3 has the adjustment effect during the osteogenic differentiation of the periodontal ligament stem cells is confirmed through screening, expression of osteogenesis key genes in hPDLSCs can be induced, the number of mineralization nodes in cells can be increased, and hPDLSCs osteogenesis induced disintegration is promoted.

The invention relates to the technical field of stem cell cryopreservation, in particular to a parodontium stem cell cryoprotectant and a cryopreservation method thereof. The parodontium stem cell cryoprotectant is prepared from, 500-2000 mg/L of trehalose, 5-20vt% of acetamide, 5-15vt% of DMSO and the balance a basal culture medium. Compared with a conventional cell cryoprotectant, the parodontium stem cell cryoprotectant and the cryopreservation method thereof have the advantages that the cryoprotectant has a less damage effect on cells during cell cryopreservation, the cell viability is higher, the cryopreservation effect of the cryoprotectant is obviously superior to that of the conventional cell cryoprotectant; on one hand, the cost of the cell cryopreservation can be reduced to a certain degree, on the other hand, the introduction of heterologous matter can be avoided, and the clinical safety is higher.

The invention relates to a periodontal ligament stem cells (PDLSCs) osteogenic differentiation inducing liquid and a method. The inducing liquid is prepared from the following components: basal culture medium, vitamin C, dexamethasone, beta-glycerophosphate sodium, FBS, IGF-1 and BMP-2. The periodontal ligament stem cell osteogenic differentiation inducing method uses the above osteogenic differentiation inducing liquid to induce. According to the multiple inducing factor osteogenic inducing liquid provided by the invention, multiple inducing factors are jointly applied in PDLSCs, so that the PDLSCs osteogenic differentiation can be effectively induced. According to the invention, the adopted raw materials are third molars required to be removed when orthodontic treatment is carried out on 12-18-year-old teenagers, and at present, the third molars are basically adopted as medical wastes to throw away; while according to the invention, the third molars are used for isolated culturing the PDLSCs to carry out osteogenic induction, so that the recycle of the medical wastes is effectively realized.

The invention provides a preparation method of a novel tissue repair-promoting material and an application thereof, particularly can be used for bone defect and repair treatment, which belong to the field of tissue engineering and regenerative medicine. A composite scaffold is prepared by mixing and freeze-drying TGF-beta3-chitosan-ossein protein microspheres and chitosan sponge containing bioactive human-like collagen protein. The composite scaffold has the characteristics of being good in biocompatibility, is capable of promoting proliferation and adhesion of cells around injured tissues andcollecting stem cells in vivo, and is suitable for cell growth and osteogenic differentiation and the like. Meanwhile, the periodontal ligament stem cells can be tightly combined with the scaffold material, so that the good growth of the cells in the scaffold material is facilitated, and the scaffold material has stronger osteogenic differentiation capacity and can be better applied to treatmentof bone defects. Wide application prospects are realized in the fields of bone tissue engineering and regenerative medicine, particularly in the aspects of bone defect treatment and repair and the like.

The invention discloses a biomembrane for guided tissue regeneration of periodontium, and a preparation method and application thereof. The method comprises the following steps: culturing periodontal ligament stem cells and preparing aseptic oxygen-rich perfluorotriethylamine emulsion; mixing the periodontal ligament stem cells and the perfluorotriethylamine emulsion to prepare cell-perfluorotriethylamine emulsion which includes 104 to 108 cells per ml of the perfluorotriethylamine emulsion; and inoculating the prepared cell-perfluorotriethylamine emulsion on a biomembrane for culture, wherein the biomembrane for guided tissue regeneration of periodontium is obtained after termination of the culture. The preparation method for the biomembrane inhibits propagation of local anacerobes while improving the early-stage survival rate of the periodontal ligament stem cells after implantation by improving a hypoxia environment in the biomembrane, so the failure rate of guided tissue regeneration is greatly reduced, the success rate of guided tissue regeneration is improved, and good economic and social benefits are obtained.

A method for regulating osteogenic differentiation of periodontal ligament stem cells based on extracellular matrix comprises the following steps: 1) isolation and identification of bone marrow cellsBMCs and periodontal ligament cells PDLCs; 2) preparation of acellular bone marrow-derived extracellular matrix B-dECM and acellular periodontal ligament derived extracellular matrix P-dECM; 3) for the acellular bone marrow-derived extracellular matrix B-dECM and the acellular periodontal ligament derived extracellular matrix P-dECM obtained in step 2), performing coating tissue culture plate TCPand the acellular periodontal ligament derived extracellular matrix P-dECM by using type IV collagen alpha-2 chain COL4A2; 4) respectively using small interfering RNA and lentivirus transfection to down-regulate and up-regulate the type IV collagen alpha-2 chain COL4A2, the characteristic of accurately regulating COL4A2 in ECM can be obtained.

The invention discloses a gene modified tissue-engineered bone as well as a preparation method and application thereof. The method for preparing the gene modified tissue-engineered bone comprises the following steps: (1) constructing a pLenti6.3-hOPG-IRES-EGFP recombinant lentiviral vector; (2) treating the recombinant vector obtained in the step (1) to obtain a recombinant lentivirus; (3) infecting a periodontal ligament stem cell by the recombinant lentivirus obtained in the step (2) to obtain the recombinant lentivirus transfected periodontal ligament stem cell; (4) in-vitro compounding the recombinant lentivirus transfected periodontal ligament stem cell obtained in the step (3) together with a beta-tricalcium phosphate scaffold material to obtain the gene modified tissue-engineered bone. The tissue-engineered bone can be used for treating periodontal defect diseases.

The invention relates to a preparation method of engineering periodontium, which is used for reconstruction of periodontium. The periodontium comprises periodontal ligament stem cells and an extracellular matrix secreted and synthesized by the periodontal ligament stem cells. The periodontal ligament stem cells are subjected to amplification culture to form multi-layered periodontal ligament stem cell assemblies, and then the periodontal ligament stem cell assemblies are arranged layer by layer and molded to form the engineering periodontium. The periodontium can form a new dentinal structure in the human body, so that periodontal defects can be repaired. The periodontium is simple in preparation, good in plasticity, moderate in cell density, abundant in extracellular matrix content, free of support material, and short in in-vitro culture time.

The invention relates to mouth wash and a preparation method thereof. The mouth wash comprises a Toll-like receptor 4 neutralizing antibody, an antibody stabilizer, a sweetening agent, scented water, a wetting agent, an anti-caries agent, deionized water, and other components. The preparation method of the mouth wash is simple. According to the mouth wash, medicine is locally used, and the effect of cutting off cell wall lipopolysaccharide is performed to inhibit inflammation and antagonize the weakening of osteogenic differentiation capacity of periodontal ligament stem cells, so as to reach the purpose of treating gingivitis and periodontitis; and meanwhile, the mouth wash has the effects of cleaning the mouth, preventing the dentes cariosus, and freshening the breath.

The invention discloses a primary separation and culture method for periodontal ligament stem cells, which comprises the following steps: separating periodontal ligaments, carrying out enzymolysis on periodontal ligament tissues by a mixed enzyme of collagenase V of which the mass-volume concentration is 0.05-0.25% and pancreatin of which the mass-volume concentration is 0.05-0.25%, carrying out primary culture on periodontal ligament stem cells, and separating the periodontal ligament stem cells by adopting a limiting dilution method. The primary separation and culture method disclosed by the invention has the following advantages: (1) the cost is reduced because the cost of the pancreatin is lower than that of dispase; (2) the digestion time is shortened, the periodontal ligaments are dense connective tissues, the affinity of the collagenase V to the connective tissues is superior to that of collagenase I, and the collagenase V and the pancreatin are combined, so that the enzymolysis effect is obviously improved.

The invention discloses application of LncRNA-TUG1 in the preparation of a drug for regulating and controlling the dryness maintenance capacity of periodontal ligament stem cells. The invention discovers that LncRNA-TUG1 play very important regulation and control roles in the dryness maintenance process of the periodontal ligament stem cells for the first time. When LncRNA-TUG1 is decreased, the proliferation, self-renewal, differentiation and transfer capacities of PDLSCs are inhibited, so that LncRNA-TUG1 is determined to be taken as a potential target for the dryness maintenance of multi-energy stem cells, the development of new drugs or factors relevant with is promoted, LncRNA-TUG1 is finally applied to field of regenerative medicines, and the true accurate medical treatment on clinic is achieved.

The invention relates to a method for immortalizing a human periodontal ligament stem cell line by using an hTERT (human telomerase reverse transcriptase) lentivirus recombinant. The method comprises the following steps: 1, culturing primary human periodontal ligament stem cells (hPDLSC); 2, establishing an immortalized genetic engineering cell line hTERT-PDLSC stably expressing hTERT genes, to be specific, infecting the immortalized genetic engineering cell line with primarily cultured PDLSC by using a lentivirus carrier expressing the hTERT genes under a certain condition, and carrying out resistance screening of puromycin to obtain the immortalized cell line stably expressing the hTERT genes, wherein the service life of the cells is prolonged to 35 generations from 3-8 generations, and thus an immortalized cell model having stable properties, favorable functions and normal phenotype is obtained. The method disclosed by the invention has a beneficial effect on research of periodontal ligament stem cells to periodontal tissue engineering as well as research and development of medicaments for effectively treating periodontitis.

The invention relates to the technical field of bone defect repair, in particular to a periodontal defect repair system containing Let-7a. The system comprises a third cell polymer and hydroxyapatite-beta tricalcium phosphate wrapped in the third cell polymer; the third cell polymer is transfected with let-7a mimic; the let-7a mimic is a double-stranded RNA, and the sequence of a sense strand of the let-7a mimic is as shown in SEQ ID NO. 2. According to the scheme, the technical problem that the effect of repairing bone defects by using periodontal ligament stem cells is poor can be solved. According to the scheme, the osteogenic differentiation capacity of the periodontal ligament stem cells is regulated and controlled by using Let-7a, and a feasible way is provided for rapid and effective repair of periodontal defects in combination with a periodontal ligament stem cell polymer form cost scheme.