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Thermosensitive polymer vesicle and preparation method and application thereof

A temperature-sensitive polymer, polymer technology, used in drug combination, drug delivery, pharmaceutical formulations, etc., can solve problems such as difficulty in controlling the concentration of gas molecules, inability to target tissues or cells, and improve orthodontic efficacy and safety. The effect of improving the ability of proliferation and differentiation, and good stability

Active Publication Date: 2019-04-16
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods of drug delivery are concentrated on direct injection of donor NaHS, sustained release of donor GYY4137, or combined application with anti-inflammatory drugs, all of which have problems such as difficulty in controlling the concentration of gas molecules, inability to target tissues or cells, etc., restricting its function.

Method used

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  • Thermosensitive polymer vesicle and preparation method and application thereof
  • Thermosensitive polymer vesicle and preparation method and application thereof
  • Thermosensitive polymer vesicle and preparation method and application thereof

Examples

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preparation example Construction

[0052] A method for preparing thermosensitive polymersomes, comprising the steps of:

[0053] (1) Synthetic steps of thermosensitive polymer:

[0054] a. Using PEO-DDMAT as a macroinitiator, add N-isopropylacrylamide (NIPAM), coumarin monomer (CMA), and free radical initiator (AIBN) into a round bottom flask, and then add an appropriate amount of The second type of solvent, mix and remove the oxygen in the reaction system, then react at 70 ° C for 24h, and terminate the reaction by exposing to air, the resulting polymer PEO-b-P (NIPAM-stat-CMA) and tert-butyl acrylate (tBA), a free radical initiator (AIBN) and a second type of solvent are mixed to remove oxygen in the reaction system, and then react at 70° C. for 24 hours, and then terminate the reaction by exposing to air to obtain a polymer solution;

[0055] b. The obtained polymer solution was rotary evaporated, dissolved, and precipitated in n-hexane three times, and dried in vacuum at 25°C to obtain a polymer powder;

...

Embodiment 1

[0069] This embodiment provides a method for preparing thermosensitive polymersomes, comprising the following steps:

[0070] (1) Synthesis steps of thermosensitive polymer PEO-b-P(NIPAM-stat-CMA)-b-PAA:

[0071] a. Using 0.68g (0.30mmol) PEO-DDMAT as a macroinitiator, 2.71g (24.00mmol) N-isopropylacrylamide (NIPAM), 1.00g (3.3mmol) coumarin monomer (CMA) , 7.38mg (0.045mmol) free radical initiator (AIBN) was added in the 25mL round bottom flask, then added 3mL of dioxane, until all the reactants were dissolved, blowing argon for 15 minutes by bubbling to remove the reaction Oxygen in the system (forming an oxygen-free environment), carry out reversible addition-fragmentation chain transfer polymerization (RAFT), the reaction temperature is 50 ° C, the reaction time is 48h, the reaction is terminated by exposure to air, and the obtained polymer PEO-b-P (NIPAM-stat-CMA) is mixed with 0.46g (3.6mmol) tert-butyl acrylate (tBA) 7.38mg (0.045mmol) free radical initiator (AIBN) and...

Embodiment 2

[0080] This embodiment provides a method for preparing thermosensitive polymersomes, comprising the following steps:

[0081] (1) Synthesis steps of thermosensitive polymer PEO-b-P(NIPAM-stat-CMA)-b-PAA:

[0082] a. With 0.68g (0.30mmol) PEO-DDMAT as a macroinitiator, 3.39g (30.00mmol) N-isopropylacrylamide (NIPAM), 1.21g (4.0mmol) coumarin monomer (CMA) , 7.38mg (0.045mmol) of free radical initiator (AIBN) was added to a 25mL round bottom flask, and then 5mL of dimethylformamide was added, and until all the reactants were dissolved, argon gas was blown for 15 minutes by bubbling to remove Oxygen in the reaction system (form an oxygen-free environment), carry out reversible addition-fragmentation chain transfer polymerization (RAFT), the reaction temperature is 60 ° C, the reaction time is 36h, the reaction is terminated by exposure to air, and the obtained polymer PEO- b-P (NIPAM-stat-CMA) is mixed with 0.51g (4.0mmol) tert-butyl acrylate (tBA), 7.38mg (0.045mmol) free radic...

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Abstract

The invention relates to a thermosensitive polymer vesicle and a preparation method and an application thereof. The thermosensitive polymer vesicle includes a vesicle body assembled by amphiphilic thermosensitive block-containing polymers, and a biotin-modified Biotin-CD146 monoclonal antibody and streptavidin (SA) targeting to periodontal ligament stem cells (PDLSCs) and combined on the vesicle body. The thermosensitive polymer vesicle can be used as a hydrogen sulfide vector for application. Compared with the prior art, the thermosensitive polymer vesicle has the PDLSCs targeting property, can improve the proliferation and differentiation ability of the PDLSCs, promotes periodontal tissue remodeling under an inflammation environment, improves the orthodontic curative effect and safety inan inflammation state, and provides technical support for clinical application of gas molecules.

Description

technical field [0001] The invention belongs to the field of polymer materials, and in particular relates to a temperature-sensitive polymer vesicle carrying a hydrogen sulfide donor and targeting periodontal ligament stem cells, a preparation method and application thereof. Background technique [0002] It is difficult for patients to maintain good oral hygiene during orthodontic treatment, which often causes periodontal tissue inflammation. If it cannot be controlled in time, it will easily lead to loss of periodontal attachment and rapid alveolar bone resorption. Even after the basic periodontal treatment is completed, the regeneration and reconstruction ability of periodontal tissue will decline, which restricts the safe and effective implementation of orthodontic treatment. During the process of orthodontic periodontal remodeling, periodontal ligament cells (PDLCs), especially the mesenchymal stem cells - periodontal ligament stem cells (PDLSCs), determine the regenerat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/68A61K47/69A61K33/04A61P1/02
CPCA61K33/04A61K47/6898A61K47/6915A61P1/02
Inventor 华咏梅杜建忠廖雨瑶王月倪可人赵逸
Owner TONGJI UNIV
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