Chitosan-extracellular matrix tissue repairing membrane and preparation method thereof

Abstract

The invention discloses a chitosan-extracellular matrix tissue repairing membrane and a preparation method thereof and belongs to the field of biomedical materials. Extracellular matrixes are uniformly distributed in a chitosan solution, and the membrane is obtained through drying. The prepared tissue repairing membrane has excellent biocompatibility and degradability, and can promote tissue regeneration and repairing and prevent tissue adhesion.

Description

technical field

[0001] The invention belongs to the field of biomedical materials, and in particular relates to a chitosan-extracellular matrix tissue repair membrane and a preparation method thereof. Background technique

[0002] Tissue and organ repair is a worldwide medical problem. Research and development of ideal materials for tissue repair has always been an important topic in the fields of medicine, bioscience and material science. At present, the tissue repair membrane products widely used in clinical practice are woven mesh, but such products have poor biocompatibility, strong immune rejection, and their structure and composition are far from those of human tissues, so they can only replace them in a short period of time. And support, can not promote tissue regeneration, to achieve tissue function.

[0003] Chitosan is the deacetylation product of natural polysaccharide chitin, which is an alkaline polysaccharide containing free amino groups. Studies at home and ...

Examples

Embodiment 1

[0020] (1) take by weighing 0.5g chitosan powder, be dissolved in 100ml concentration and be in the acetic acid solution of 1%, stir until dissolving completely;

[0021] (2) Get the acellular small intestine submucosa matrix of pigs, grind and pulverize, and filter through a 200-mesh sieve to obtain pig small intestine submucosa extracellular matrix powder;

[0022] (3) Take by weighing 0.05g porcine small intestine submucosa extracellular matrix powder, add in the chitosan solution, stir until the extracellular matrix powder is evenly mixed in the chitosan solution;

[0023] (4) Pour the chitosan-porcine small intestinal submucosa extracellular matrix solution into a mold, and dry it in an oven at low temperature to form a film to obtain the chitosan-porcine small intestinal submucosa extracellular matrix membrane. The prepared chitosan-porcine small intestinal submucosa extracellular matrix membrane is shown in the accompanying drawing.

Embodiment 2

[0025] (1) take by weighing 2g chitosan powder, be dissolved in 100ml concentration and be in the acetic acid solution of 5%, stir until dissolving completely;

[0026] (2) Get the bovine Achilles tendon decellularized matrix, grind and pulverize, and filter through a 400-mesh sieve to obtain bovine Achilles tendon extracellular matrix powder;

[0027] (3) Take by weighing 1g of bovine Achilles tendon extracellular matrix powder, add it to the chitosan solution, and stir until the extracellular matrix powder is evenly mixed in the chitosan solution;

[0028] (4) Pour the chitosan-bovine Achilles tendon extracellular matrix solution into the mold, and dry it in an oven at low temperature to form a film to obtain the chitosan-bovine Achilles tendon extracellular matrix membrane.

Embodiment 3

[0030] (1) take by weighing 1.5g chitosan powder, be dissolved in 100ml concentration and be in the acetic acid solution of 3%, stir until dissolving completely;

[0031] (2) get the porcine peripheral nerve acellular matrix, grind and pulverize, and filter with a 300-mesh sieve to obtain porcine peripheral nerve extracellular matrix powder;

[0032] (3) Take by weighing 0.5g porcine peripheral nerve extracellular matrix powder, add in the chitosan solution, stir until the extracellular matrix powder is evenly mixed in the chitosan solution;

[0033] (4) Pour the chitosan-porcine peripheral nerve extracellular matrix solution into a mold, and dry it in an oven at low temperature to form a film to obtain the chitosan-pig peripheral nerve extracellular matrix membrane.

technical field

[0001] The invention belongs to the field of biomedical materials, and in particular relates to a chitosan-extracellular matrix tissue repair membrane and a preparation method thereof. Background technique

[0002] Tissue and organ repair is a worldwide medical problem. Research and development of ideal materials for tissue repair has always been an important topic in the fields of medicine, bioscience and material science. At present, the tissue repair membrane products widely used in clinical practice are woven mesh, but such products have poor biocompatibility, strong immune rejection, and their structure and composition are far from those of human tissues, so they can only replace them in a short period of time. And support, can not promote tissue regeneration, to achieve tissue function.

[0003] Chitosan is the deacetylation product of natural polysaccharide chitin, which is an alkaline polysaccharide containing free amino groups. Studies at home and ...

Examples

Embodiment 1

[0020] (1) take by weighing 0.5g chitosan powder, be dissolved in 100ml concentration and be in the acetic acid solution of 1%, stir until dissolving completely;

[0021] (2) Get the acellular small intestine submucosa matrix of pigs, grind and pulverize, and filter through a 200-mesh sieve to obtain pig small intestine submucosa extracellular matrix powder;

[0022] (3) Take by weighing 0.05g porcine small intestine submucosa extracellular matrix powder, add in the chitosan solution, stir until the extracellular matrix powder is evenly mixed in the chitosan solution;

[0023] (4) Pour the chitosan-porcine small intestinal submucosa extracellular matrix solution into a mold, and dry it in an oven at low temperature to form a film to obtain the chitosan-porcine small intestinal submucosa extracellular matrix membrane. The prepared chitosan-porcine small intestinal submucosa extracellular matrix membrane is shown in the accompanying drawing.

Embodiment 2

[0025] (1) take by weighing 2g chitosan powder, be dissolved in 100ml concentration and be in the acetic acid solution of 5%, stir until dissolving completely;

[0026] (2) Get the bovine Achilles tendon decellularized matrix, grind and pulverize, and filter through a 400-mesh sieve to obtain bovine Achilles tendon extracellular matrix powder;

[0027] (3) Take by weighing 1g of bovine Achilles tendon extracellular matrix powder, add it to the chitosan solution, and stir until the extracellular matrix powder is evenly mixed in the chitosan solution;

[0028] (4) Pour the chitosan-bovine Achilles tendon extracellular matrix solution into the mold, and dry it in an oven at low temperature to form a film to obtain the chitosan-bovine Achilles tendon extracellular matrix membrane.

Embodiment 3

[0030] (1) take by weighing 1.5g chitosan powder, be dissolved in 100ml concentration and be in the acetic acid solution of 3%, stir until dissolving completely;

[0031] (2) get the porcine peripheral nerve acellular matrix, grind and pulverize, and filter with a 300-mesh sieve to obtain porcine peripheral nerve extracellular matrix powder;

[0032] (3) Take by weighing 0.5g porcine peripheral nerve extracellular matrix powder, add in the chitosan solution, stir until the extracellular matrix powder is evenly mixed in the chitosan solution;

[0033] (4) Pour the chitosan-porcine peripheral nerve extracellular matrix solution into a mold, and dry it in an oven at low temperature to form a film to obtain the chitosan-pig peripheral nerve extracellular matrix membrane.

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