303 results about "Free amino" patented technology

The invention discloses cured beef and a preparation method thereof, and belongs to the technical field of fermented food. Comprising the following steps: trimming and dicing beef, adding seasoning spices, and adding a microbial agent for fermentation, the microbial agent is prepared from lactobacillus plantarum NR1-7 and aroma-producing yeast. According to the invention, the cured beef fermented by adding the Chinese prickly ash extracting solution, the lactobacillus plantarum NR1-7 and the aroma-producing yeast is relatively high in viable count, good in color and luster, rich in free amino acid quantity, moderate in protease activity and appropriate in organic acid content, and the pH value is stabilized to be about 5.5. Compared with natural fermentation, the growth and reproduction of inoculated dominant bacteria can accelerate the degradation of beef components and generate rich flavor, the pepper extract can ensure the complete beef structure by weakening the activity of endogenous enzyme, and in addition, the sour taste can be alleviated, the excessive degradation of fat can be relieved, and the fermentation quality of the cured beef can be improved.

The application discloses an Aspergillus niger strain and a culture method and application thereof, the Aspergillus niger strain is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20241727. The fermented tea product obtained by fermenting tea leaves or tea leaf extracts by using the Aspergillus niger strain CCTCC NO: M 20241727 has prominent sweet and floral fragrance, and the astringency is obviously reduced; the contents of tea polyphenols, free amino acids and flavones are higher than those of a fermented tea product of an existing Aspergillus niger strain; the antioxidant activity is enhanced; the tea soup color is orange red and bright; and the color, fragrance and taste qualities are all superior to those of the fermented tea product of the existing Aspergillus niger strain.

The invention discloses Kluffa yeast and application of the Kluffa yeast in preparation of red camellia fermentation liquor with anti-aging and repairing functions, the Kluffa yeast is used for repairing PR-LQX002, the Latin name of the Kluffa yeast is Lachancea kluyveri, and the preservation number of the Kluffa yeast is CCTCC NO: M 20241723. The invention also provides a red camellia fermentation broth, a composition containing the fermentation broth and application of the red camellia fermentation broth in preparation of products with anti-oxidation, anti-saccharification, anti-wrinkle or repair effects. The fermentation broth is prepared from the following raw materials: red camellia flowers, red camellia leaves and Kluffa yeast PR-LQX002. The Kluffa yeast can greatly promote fermentation of flowers and leaves of the red camellia, and the obtained red camellia fermentation liquor not only contains active substances such as flavone, polyphenol and free amino acid, but also contains lipid substances such as ceramide, and has multiple effects such as saccharification resistance, repair, wrinkle resistance and oxidation resistance.

The invention relates to the technical field of agricultural fertilizer preparation, and discloses a preparation method of a water-soluble fertilizer rich in various active amino acids, which comprises the following steps: S1, preparing an amino acid solution: dissolving various free amino acids or polypeptides in a buffer solution to form an amino acid precursor liquid; s2, constructing a nutrition core layer: adding one or more types of soluble trace element salts and a gelation auxiliary agent into the amino acid precursor liquid to form gelatinous particles; s3, forming an intermediate response layer: coating the core particles with chitosan and phospholipid to form a bimolecular membrane structure. The stability of the particle structure is improved through low-temperature crosslinking and bi-component gelation, and quick response and continuous controlled release in a weak acid environment are realized by combining an intermediate response membrane and an outer controlled release shell layer; by adopting a structure-function integrated design, the suspension property and the dispersity are remarkably improved, and the multi-crop and multi-period precise fertilization requirements are met.

The invention belongs to the technical field of immunodetection, and discloses a kit for detecting the ratio of proBDNF to maure BDNF based on structural dynamics guidance and a preparation method of the kit, the kit comprises a solid phase carrier and a detection reagent, the three antibodies are determined on the basis of full-length conformational kinetics analysis of brain-derived neurotrophic factors; the first capture antibody targets an innate disorder region (SEQ ID NO: 1) of a proBDNF propeptide region; the second capture antibody targets an innate disorder region (SEQ ID NO: 2) of a proBDNF propeptide region; the second capture antibody is a neo-epitope specific antibody, is specifically combined with an N terminal (SEQ ID NO: 2) exposed by maure BDNF enzyme digestion, and is combined with a free alpha-amino group strictly dependent on the first histidine; and the universal detection antibody is combined to a Loop 4 region (SEQ ID NO: 3) of the Mature structural domain. The problems of steric hindrance and cross reaction in traditional immunodetection are solved through a structural biology strategy, and accurate distinguishing and ratio quantification of proBDNF and maure BDNF are achieved.

The invention provides a method for detecting free amino acids in camellia oleosa seed oil, and establishes a method for detecting the free amino acids in the camellia oleosa seed oil based on ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS), which comprises the following steps: pre-treating various amino acids in the camellia oleosa seed oil by using a non-derivation method; the problems of complicated operation, impurity interference and the like caused by derivatization are avoided, the extraction solvent is environment-friendly and economical, the extraction efficiency is high, and the pretreatment time is greatly shortened; then selecting a proper chromatographic column and a mobile phase, separating a plurality of free amino acids under the condition of not introducing excessive derivatization agents and ion pair reagents, and controlling the collection time to be 12 minutes. The method for detecting the free amino acids in the camellia oleosa seed oil has the characteristics of no need of derivatization treatment, simplicity and convenience in operation, high detection efficiency and good sensitivity and accuracy, and can effectively separate and detect 14 free amino acids such as alanine, phenylalanine and aspartic acid in the camellia oleosa seed oil.

The invention belongs to the technical field of food processing, and particularly relates to fermented beef jerky and a preparation method thereof. The preparation method of the beef jerky comprises the following steps: (1) removing fascia and fat from fresh cattle hind leg meat, washing with clear water, and cutting into meat slices; (2) adding table salt, glucose, white granulated sugar and sodium nitrite into the sliced meat, uniformly mixing, adding a fermentation inoculant which comprises staphylococcus calcaratus, pediococcus acidilactici, bifidobacterium bifidum and Witzia ciliaris, and fermenting; and (3) after fermentation, drying and cooling to obtain the fermented beef jerky. Specific strains are selected to form a fermentation inoculant for fermentation, and the prepared fermented beef jerky is high in free amino acid and soluble peptide content, low in nitrite residual quantity, uniform red brown in color and slightly bright in surface, and belongs to high-quality beef jerky.

The present invention belongs to the field of cell culture media. Provided are a method for preparing a stem cell culture medium by using a protein hydrolysate as a core raw material and a method of using the stem cell culture medium. The stem cell culture medium contains 20-80% of the protein hydrolysate on the basis of the total weight of the components in the stem cell culture medium. The protein hydrolysate comprises one or more of a high-free amino acid yeast hydrolysate, a high-nucleotide yeast hydrolysate, a high-glutathione yeast hydrolysate, a high-polypeptide yeast hydrolysate, a rice protein hydrolysate, a high-glutamine wheat protein hydrolysate, a pea protein hydrolysate and a soybean protein hydrolysate. The preparation of a natural animal stem cell culture medium by using a whole nutrient protein hydrolysate as a core raw material can meet the nutritional requirements for animal stem cell proliferation under the conditions of no additional addition or addition of a small amount of amino acids, vitamins, growth factors and serum, which effectively reduces the cost of the stem cell culture medium, thereby providing the stem cell culture medium with wide application prospects in the field of foods such as cultured meat.

The invention relates to the technical field of organic fertilizers, in particular to an alginic acid organic fertilizer and a preparation method thereof.The alginic acid organic fertilizer is prepared from 450-550 g / L of seaweed extract, 80-110 g / L of free amino acid, 80-110 g / L of nitrogen (N), 80-110 g / L of phosphorus (P2O5), 80-110 g / L of potassium (K2O), 40-60 g / L of microbial agent and microelements, and the alginic acid organic fertilizer can effectively improve the yield and quality of strawberries. According to the preparation method, complex instruments and equipment and special reaction conditions are not needed in the preparation process of the alginic acid organic fertilizer, large-scale production is easy, the production cost is reduced, the production efficiency is improved, and popularization and application of the product in the market are facilitated.

The invention discloses an amino acid water-soluble fertilizer and a production process thereof, and relates to the technical field of water-soluble fertilizers. Free amino acid is used as an organic nitrogen source, and the medium element calcium is combined, so that the crop quality is improved. The formula is low in cost, high in absorptivity, suitable for crop growth, simple and convenient to use and high in cost performance, and promotes the synergistic effect of nutrient elements. The method takes amino acid and calcium as raw materials, and has the characteristics of wide applicability, high output-input ratio and easiness in market promotion. The product is completely water-soluble, environment-friendly and safe, can regulate the physiological function of crops, increase the yield and amino acid content and improve the quality, and has remarkable economic benefits. In addition, activated ricinoleic acid carboxyl is combined with fucoidan enzymatic hydrolysis oligosaccharide to construct the microcapsule, so that efficient utilization of resources is realized.

The invention provides a method for preparing a compound amino acid chelated slow-release fertilizer from waste spirulina mud, and belongs to the technical field of agricultural fertilizers. According to the method disclosed by the invention, the waste spirulina mud from which phycocyanin and polysaccharide are extracted is fully utilized, the waste spirulina mud is decomposed into free amino acid, and the free amino acid is compounded with marine polysaccharide to prepare a green compound amino acid chelated fertilizer product with a slow-release effect; secondly, the nutrient release period of the fertilizer can be regulated and controlled by controlling the proportion and concentration of the marine polysaccharide, and the fertilizer has excellent water and fertilizer retention capacity.

Provided herein are methods for treating a pulmonary infection in a patient in need thereof, for example, a nontuberculous mycobacterial pulmonary infection for at least one treatment cycle. The method comprises administering to the lungs of the patient a pharmaceutical composition comprising a liposomal complexed aminoglycoside comprising a lipid component comprising electrically neutral lipids and an aminoglycoside. Administration comprises aerosolizing the pharmaceutical composition to provide an aerosolized pharmaceutical composition comprising a mixture of free aminoglycoside and liposomal complexed aminoglycoside, and administering the aerosolized pharmaceutical composition via a nebulizer to the lungs of the patient. The methods provided herein result in a change from baseline on the semi-quantitative scale for mycobacterial culture for a treated patient, and / or NTM culture conversion to negative during or after the administration period.

Methods for making wettable, dispersible potato protein powders are provided. The wettable dispersible potato protein powders comprise potato protein, free amino acids, non-protein nitrogen compounds, and minerals from potatoes; and are low in total α-glycoalkaloids. The inventive potato protein powders are suitable for use in formulating foods, feeds and beverages.

The invention discloses a crop quality index content prediction method, system and device, a medium and a product, and relates to the technical field of crop quality index detection.The method comprises the steps that near infrared spectrum data and conductivity data of a to-be-detected crop sample are obtained and preprocessed, and the conductivity of the to-be-detected crop sample is obtained; carrying out characteristic wave band screening on the preprocessed near infrared spectrum data to obtain screened near infrared spectrum data, and fusing the screened near infrared spectrum data and the preprocessed conductivity data to obtain a fused characteristic vector of the to-be-detected crop sample; respectively inputting the fusion feature vectors of the to-be-detected crop samples into the crop quality index content prediction models to obtain corresponding predicted values of the crop quality index content; the crop quality index content is free amino acid content, starch content, soluble sugar content or vitamin C content. The accuracy and the prediction efficiency of crop quality index content prediction are improved.

The present invention relates to: a Bacillus amyloliquefaciens Mu2 strain; and a chlorella protein extraction method using same. A chlorella protein extracted using the strain can be effectively used as an alternative protein material containing an abundance of constituent amino acids and free amino acids.

Provided herein are methods for treating a pulmonary infection in a patient in need thereof, for example, a nontuberculous mycobacterial pulmonary infection for at least one treatment cycle. The method comprises administering to the lungs of the patient a pharmaceutical composition comprising a liposomal complexed aminoglycoside comprising a lipid component comprising electrically neutral lipids and an aminoglycoside. Administration comprises aerosolizing the pharmaceutical composition to provide an aerosolized pharmaceutical composition comprising a mixture of free aminoglycoside and liposomal complexed aminoglycoside, and administering the aerosolized pharmaceutical composition via a nebulizer to the lungs of the patient. The methods provided herein result in a change from baseline on the semi-quantitative scale for mycobacterial culture for a treated patient, and / or NTM culture conversion to negative during or after the administration period.

The application discloses a composition, which comprises hydrolyzed protein; the hydrolyzed protein comprises hydrolyzed whey protein and hydrolyzed casein protein, the weight ratio of the hydrolyzed whey protein and the hydrolyzed casein protein is (70-95):(5-30), the amino acid nitrogen content in the hydrolyzed protein is (1.0-3.0) g / 100 g, the proportion of protein hydrolysate with a relative molecular mass distribution <1000 in the hydrolyzed protein is 34-70%, the peptide content (calculated on a dry basis) in the hydrolyzed protein is not less than 33 g / 100 g, and the total free amino acid content in the hydrolyzed protein is not less than 1 g / 100 g. The composition can effectively treat diarrhea and is suitable for people with gastrointestinal dysfunction and severe patients with diarrhea symptoms.

This invention discloses a polyacrylic acid / chitosan composite hydrogel loaded with resveratrol liposomes and laver fermentation broth, its preparation method, and its application, belonging to the field of gel cosmetics technology. This invention uses acrylic acid and chitosan as substrates, and employs Fe... 3+ This invention innovatively employs a physical cross-linking strategy to synergistically load resveratrol liposomes and seaweed fermentation broth, providing a composite hydrogel with excellent matrix properties, high active ingredient penetration rate, and multiple functions. Utilizing the abundant low-molecular-weight polysaccharides, polyphenols, and free amino acids in seaweed fermentation broth, this invention not only enhances the three-dimensional water-retaining network of the composite gel but also significantly promotes collagen synthesis and exerts anti-UV and anti-allergic soothing effects. The invention also provides a preparation process for the composite hydrogel. The prepared composite hydrogel effectively encapsulates resveratrol liposomes with the fermentation broth-containing hydrogel, exhibiting a certain degree of sustained-release properties. While protecting the stability of the active ingredients, it allows the active ingredients to gradually penetrate to the deep epidermis and even the dermis, achieving non-irritating and highly efficient utilization. The polyacrylic acid / chitosan composite hydrogel loaded with resveratrol liposomes and seaweed fermentation broth described in this invention possesses multiple functions such as antibacterial, moisturizing, whitening, antioxidant, and soothing repair, meeting people's daily skincare needs and showing promising market prospects.

The invention discloses a method for producing soy sauce by using termitomyces albuminosus, and belongs to the technical field of soy sauce production. Aspergillus oryzae and aspergillus niger are subjected to multi-generation domestication screening through a termitomyces albuminosus powder culture medium, the adaptability of the strain to a termitomyces albuminosus substrate is remarkably improved, termitomyces albuminosus has antioxidant activity, the domesticated strain can fully utilize nutritional ingredients in the termitomyces albuminosus in the fermentation process, generation of termitomyces albuminosus flavor substances is promoted, and the yield of the termitomyces albuminosus flavor substances is increased. A compound enzyme system of neutral protease, cellulase, flavourzyme and laccase is adopted, gradient enzyme deactivation and ultrasonic-assisted treatment are combined, cell walls and protein of termitomyces albuminosus are fully decomposed, free amino acid, polysaccharide and flavoring substances are released, the enzymolysis efficiency is improved, and the flavor of the termitomyces albuminosus is improved; through secondary saccharification of the red date polysaccharide extract and beta-glucanase, the content of functional polysaccharide and soluble sugar in the soy sauce is increased, the sour taste of the soy sauce is balanced, and the taste roundness is improved.

Antibody formulations are described comprising a mixture of an anti-α4β7 antibody, an antioxidant or chelator, and at least one free amino acid. The disclosed formulations may have improved stability, reduced aggregate formation, or both. The present invention further provides a safe dosing regimen of these antibody formulations that is easy to follow, and which results in a therapeutically effective amount of the anti-α4β7 antibody in vivo.

The invention relates to a preparation method of free amino acid. The preparation method comprises the following step: in methanol, in the presence of an alkaline lithium reagent, carrying out a neutralization reaction on amino acid hydrochloride to prepare the free amino acid. The preparation method is simple and feasible, high in yield and low in cost, and free amino acid can be prepared from amino acid hydrochloride in a small amount or in an industrialized mode.

Belongs to the field of cell culture media. The stem cell culture medium contains 20-80% of protein hydrolysate on the basis of the weight of the total components in the stem cell culture medium. The protein hydrolysate comprises one or more of a high free amino acid yeast hydrolysate, a high nucleotide yeast hydrolysate, a high glutathione yeast hydrolysate, a high polypeptide type yeast hydrolysate, a rice protein hydrolysate, a high glutamine wheat protein hydrolysate, a pea protein hydrolysate and a soybean protein hydrolysate. The animal stem cell natural culture medium is prepared by taking the full-nutrition protein hydrolysate as a core raw material, so that the nutritional requirements of animal stem cell proliferation can be met under the condition of not additionally adding or adding a small amount of amino acids, vitamins, growth factors and serum, and the cost of the stem cell culture medium is effectively reduced; therefore, the method has a wide application prospect in the field of food such as cell culture meat.

The invention belongs to the technical field of food processing, and particularly discloses a preparation process of natural preservative chicken soup based on lactobacillus fermentation. The process mainly comprises the following steps: decocting chicken and water according to a specific ratio to obtain basic chicken soup; the preparation method comprises the following steps: adding lactose, a yeast extract and the like as fermentation substrates, sterilizing, and inoculating a compound lactic acid bacteria starter consisting of lactobacillus plantarum and pediococcus pentosaceus to carry out anaerobic directional fermentation until the pH value is reduced to 4.0-4.6; and after fermentation is completed, adding natural preservative enhancers such as lactoferrin, and finally, homogenizing and filling to obtain the product. Natural preservation and flavor improvement of the chicken soup are synchronously achieved through a biological fermentation method, the obtained product is high in bacteriocin yield and remarkable in pathogenic bacterium inhibition effect, long-acting preservation can be achieved without adding chemical preservatives, meanwhile, the content of free amino acid and umami substances is greatly increased, and the fresh-keeping effect is good. The method is suitable for industrial production of high-quality concentrated chicken soup seasoning and liquid soup base.

The invention belongs to the technical field of composite membrane preparation, and particularly relates to a salmon skin gelatin composite membrane with high mechanical performance and a preparation method thereof. Salmon skin gelatin, a chitosan solution, absolute ethyl alcohol, glycerin, sorbitol, montmorillonite, silicon dioxide, anhydrous sodium sulfite and a genipin acetic acid aqueous solution are used as raw materials, salmon skin gelatin protein and chitosan have good compatibility, protein molecules are fully stretched in the heat treatment denaturation process, the water absorption hydraulic radius of the protein molecules is increased, and the water absorption rate of the protein molecules is increased. The molecules interact with each other to form a compact space gel net structure; glycerol and sorbitol enhance the strength and toughness of the composite film; montmorillonite and silicon dioxide are beneficial to improving the mechanical strength of the composite film; in addition, the genipin acetic acid aqueous solution is added to modify free amino groups on salmon skin gelatin protein molecules, so that the interaction force among molecules is improved. Therefore, after the raw materials are specifically combined, synergistic interaction is achieved, and the mechanical performance of the salmon skin gelatin composite film is improved.

The present invention relates to nutritional compositions comprising digestible carbohydrates, lipids, proteins, 3-fucosyl lactose (3-FL) and a butyrate source, which are suitable for providing nutrition to an infant or young child suffering from or at risk of developing a food allergy, preferably cow milk protein or egg allergy, wherein the protein is or consists essentially of hydrolysed protein and / or free amino acids, and wherein the butyrate is provided in the form of dietary butyrate and / or by non-digestible saccharides selected from non-digestible polysaccharides, non-digestible oligosaccharides or mixtures thereof capable of undergoing microbial fermentation in the gastrointestinal tract to produce butyrate, and uses thereof.

The application discloses a kind of umami extract, belong to food field.The preparation method of the umami extract with clam meat as raw material, after fermentation by specific strain, then the fermentation liquor is subjected to enzymolysis by specific composition of composite enzyme, filtration, irradiation treatment and freeze-drying is prepared.The step of fermentation before enzymolysis in the present application, through the synergistic effect of microorganism and composite enzyme can effectively avoid the generation of bitter taste, fishy smell and other undesirable flavors in extract, and the free amino acid content of the umami extract prepared is significantly increased, and the percentage of glutamic acid, aspartic acid and other umami amino acids in total free amino acid is also significantly improved.In addition, the present application also proves by experiment that adding amylose, sugar cane molasses and bonito powder in clam meat slurry during fermentation can further increase the content of free amino acid and the percentage of umami amino acid in umami extract, so that the flavor of the umami extract prepared is better.

The present invention provides a composition comprising the reaction product of a) itaconic anhydride; and b) an amino compound comprising at least one free amino group, wherein the amino compound is selected from hydrolysed proteins; wherein the composition comprises itaconic modified amino compound, wherein the itaconic modified amino compound is the reaction product of a) and b). In another embodiment, the amino compound is selected from the group consisting of amino acids, peptides and hydrolysed proteins. The invention further provides a home care formulation comprising the composition of the invention, a fabric conditioner or softener, a method of treating fibres and a use of the composition.

The method comprises the following steps: drying and crushing shrimp heads and shrimp shells, adding water according to a ratio of 1: 3, adding 0.5%-0.7% of compound protease, and carrying out enzymolysis at 50-55 DEG C for 2-3 hours; after enzyme deactivation, adding 7-10% of brown sugar to adjust the pH value to 6.0-6.5, and sterilizing; the method comprises the following steps: inoculating pineapple endophytic lactic acid bacteria (CGMCC 25352), hansenula polymorpha (CGMCC 25353) and aspergillus oryzae, mixing the three strains according to the viable count of 1: 1: 1 (the total inoculum size is 3-4.5%, and the CFU / g is 10-10), and fermenting for 46-48 hours at the temperature of 30-32 DEG C in a dark place; and filtering and storing at-80 DEG C. The product is free of salt addition, the fermentation period is less than or equal to 48 hours, the amino acid nitrogen content is greater than or equal to 0.51 g / 100g, the total content of lactic acid (6.88 mg / g), succinic acid (3.73 mg / g) and free amino acid is greater than or equal to 313mg / 100g, the volatile amino salt content is less than or equal to 38.72 mg / 100mL, and the product has salty and fresh flavor and orange-yellow clarified appearance. The problems of high salt, long period and heavy fishy smell of the traditional shrimp soy sauce are solved.

The invention provides an aroma-enhancing and thorn-reducing tobacco extract, a preparation method thereof and a cigarette product. The preparation method of the tobacco extract comprises the following steps: mixing tobacco dry distillation liquid, molasses caramelized feed liquid and free amino acid to prepare mixed feed liquid; the mixed material liquid is subjected to a heating reaction, and the tobacco extract is prepared; wherein the temperature of the heating reaction ranges from 100 DEG C to 140 DEG C, and the time of the heating reaction ranges from 20 min to 60 min. The tobacco dry distillation liquid, the molasses caramelized feed liquid and the free amino acid are mixed and subjected to the Maillard reaction under the specific heating condition, the content of irritant substances such as phenols in the finally prepared tobacco extract can be greatly reduced, and the tobacco extract is softer and more natural in smell; meanwhile, beneficial components in the tobacco can be reserved, so that the prepared tobacco extract is richer, richer and more lasting in aroma.

The invention belongs to the technical field of lubricating oil, and particularly relates to a lubricating oil composition as well as a preparation method and application thereof. The lubricating oil composition comprises the following raw materials in parts by weight: 0.1-5 parts of a hydrocarbon compound, 0.1-5 parts of an emulsifier and 90-99.8 parts of a water phase, the water phase comprises the following raw materials: a compound containing a free amino functional group, an alcohol compound and water. The lubricating oil composition has the beneficial effects that the lubricating oil composition has good water-phase cooling and cleaning performance, abrasive dust and heat generated in the machining process can be effectively removed, and heating deformation of the metal surface is prevented. Meanwhile, the lubricating property of an oil phase is also kept, a stable oil film is formed on the surface of bearing steel, direct contact of the metal surface is prevented, the friction coefficient is remarkably reduced, and abrasion is reduced.