1376 results about "Free amino" patented technology

An emulsifying system for elemental diet compositions is provided that comprises a protein source that may be a extensively hydrolyzed protein, free amino acids, short-chain peptides, or a mixture thereof; a lipid source; a carbohydrate source; and an emulsifying system comprising an ocentyl succinic anhydride modified starch and an acetylated monoglyceride emulsifier. The composition may be prepared as a powder or liquid nutritional composition for formulas prepared for infant, pediatric and adult populations in need of an elemental diet. The invention provides a commercially acceptable product in terms of desired stability and physical characteristics and is particularly useful for hypoallergenic infant formula preparations.

The present invention relates to methods for the prevention and treatment of chronic inflammatory diseases, cancer, and involuntary weight loss. In the practice of the present invention patients are enterally administered HMB alone or alternatively in combination with eicosapentaenoic (20:5 ω-3), FOS, carnitine and mixtures thereof. HMB may be added to food products comprising a source of amino-nitrogen enriched with large neutral amino acids such as leucine, isoleucine, valine, tyrosine, threonine and phenylalanine and subtantially lacking in free amino acids.

The methods and compositions disclosed herein concern the synthesis of a novel class of “two-headed” phospholipid-phosphoinositide hybrids possessing a carbon backbone, such as 2,3-diacylthreitol, erythritol or a synthetic module. The second phospholipid head group allows introduction of a biochemical or chemical moiety in a position orthogonal in space to those occupied by the phosphoinositide head group and the two acyl chains. The diacyl moieties allow for the incorporation of Pea-PIP2 into a lipid bilayer, while the Ptdlns(4,5)P2 moiety in the aqueous layer is specifically recognized by lipid binding proteins. In alternative embodiments of the invention, reporters, for example biotin, fluorophores and / or spin labels, are attached to the free amino group of the head groups of such molecules to specifically target the reporters to the lipid-water interface.

Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate dehydrogenase (GDH) gene is introduced into a plant together with a regulator sequence suitable for over expressing the sequence encoding GDH gene in plant cells.

The present invention describes protein hydrolysates: obtainable by the hydrolysis of a protein containing substrate; comprising free amino acids and peptides; and wherein the molar fraction of at least one free amino acid, present in the protein hydrolysate is at least a factor 2.5, preferably at least a factor 3, more preferably at least a factor 3.5 times higher than in a hydrolysate of the same protein containing substrate which has been completely hydrolysed to free amino acids, wherein the molar fraction of the at least one free amino acid in the protein hydrolysate is at least 25%; and wherein the Amino Acid Quotient (AAQ) in the protein hydrolysate is at least 10%. These protein hydrolysates can be used in the preparation of food compositions, wherein these protein hydrolysates provide for novel and unexpected flavours. Moreover these protein hydrolysates are applicable in personal care applications.

The invention relates to a method for preparing health-benefiting fermented soybean meal enriched in small peptides, gamma-aminobutyric acid (GABA), reducing sugar, lactobacillus plantarum and antimicrobial peptide. By utilizing strains of aspergillus oryzae, saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum, the small peptides, free amino acids, reducing sugar, GABA and high viable count content in the lactobacillus plantarum in the health-benefiting fermented soybean meal are obtained through the two-stage solid state fermentation method, the small peptides account for 8.01-18 percent, the free amino acids has the content of 80-300mg / g, the reducing sugar reaches 20-80mg / g, the GABA reaches 0.317-0.5mg / g, the viable count content in the lactobacillus plantarum is 2-6.5*1010cfu / g, the antibacterial titer of antimicrobial peptides is 2.0*103-7.0*104 IU / g, and the yield of the health-benefiting fermented soybean meal is 82.03-90 percent. According to the process, the nutrient effect of the soybean meal can be improved, the prepared fermented soybean meal has the antibacterial effect, partial antibiotics can be replaced, the fermented soybean meal is conveniently preserved and saved, the process is simple and feasible, and the fermented soybean meal is green and environment-friendly and has application significance and wide prospect in the feed industry and breeding industry.

Glycopeptide having at least one asparagine-linked oligosaccharide at a desired position of the peptide chain obtained by: (1) esterifying hydroxyl of a resin and carboxyl of an amino acid having amino group nitrogen protected with a fat-soluble protective group (AGFPG), (2) removing the protective group to form a free amino group, (3) amidating the free amino group and carboxyl of an amino acid having AGFPG, (4) removing the protective group, (5) repeating the steps (3) and (4), (6) amidating the free amino group and carboxyl of the asparagine portion of an asparagine-linked oligosaccharide having AGFPG, (7) removing the protective group, (8) amidating the free amino group and carboxyl of an amino acid having AGFPG, (9) repeating steps (7) and (8), (10) removing the protective group, and (11) cutting off the resin with an acid; glycopeptide obtained by transferring sialic acid or a derivative thereof to the above glycopeptide.

The invention discloses a manufacturing method of paraffin sections of zostera marina embryo, comprising the following steps: (1) material drawing and fixation: zostera marina seeds are taken and the fruit skin is stripped, and after endosperm is removed, an integral zostera marina embryo is soaked in FAA (free amino acids) fixing solution and is fixed for more than 48 hours; (2) dehydration: the zostera marina embryo is taken out from the fixing solution and is soaked in alcohol for dehydration; (3) waxing and embedding: paraffin is added gradually in a vessel containing the zostera marina embryo and dimethylbenzene, and conventional paraffin embedding is carried out to obtain the wax blocks containing the zostera marina embryo; (4) slicing, spreading and drying: the wax blocks coated with the zostera marina embryo is fixed on a wheel rotation type slicing machine for carrying out continuous slicing of the paraffin, so as to obtain wax bands and enable the wax bands to be attached to an object slide; (5) dewaxing, rehydration and dyeing of hematoxylin dye solution; (6) slicing, dehydration and dyeing of eosin and alcohol; and (7) transparence and mounting are carried out to manufacture a permanent section. In the manufacturing method disclosed by the invention, the paraffin sections of the zostera marina embryo, which have clear dyeing and an integral texture structure, can be obtained.

The invention belongs to a water-soluble fertilizer in the field of agriculture production, in particular relating to an amino acid complexing various nutrient elements water-soluble fertilizer and a preparation method thereof. The water-soluble fertilizer is prepared by compexing the following raw materials in parts by weight: 48-52 parts of composite amino acid solution, 4-24 parts of macroelement compound, 4-25 parts of medium element compound, 14-34 parts of microelement compound, 2.9 parts of beneficial element compound, 2 parts of rare earth compound (rare earth nitrate with more than or equal to 38% of rare earth) and 4.1-6.2 parts of auxiliary, wherein the composite amino acid solution is a solution containing more than or equal to 26% of free amino acid and more than or equal to 12% nitrogen as well as water. The preparation method comprises seven steps and can be used for further improving the absorption utilization of a plant; and the prepared fertilizer has the advantages of full nutrition and high efficiency.

The invention discloses a preparation method of biocompatible gamma-polyglutamic-acid hydrogel, which comprises the following steps of: by using polyglutamic acid as a main raw material and a biological-source material chitosan oligosaccharide as a cross-linking agent, carrying out preparation reaction in an aqueous solution, and activating a gamma-polyglutamic-acid carboxyl group by utilizing activating agents diamine carbide and N-hydroxysuccinimide ester to enable the polyglutamic acid and the free amino group of the chitosan oligosaccharide to form an amido bond to further form the hydrogel in a three-dimensional netted structure. After the prepared gel is swelled and balanced, drying is carried out by adopting a freeze-drying, vacuum-drying or microwave-drying mode. The biological-source material chitosan oligosaccharide is selected as the cross-linking agent, so that the biocompatibility of an obtained hydrogel system can be markedly improved; and a reaction system is an aqueous-solution system, reaction regulating and controlling properties are good, and the gel is stable. Because the hydrogel prepared by the method disclosed by the invention has good biocompatibility, the hydrogel can be widely applied to medicament carriers, auxiliary wound materials, medical and sanitary articles and the like.

The present invention relates to methods for the prevention and treatment of chronic inflammatory diseases, cancer, and involuntary weight loss. In the practice of the present invention patients are enterally administered HMB alone or alternatively in combination with eicosapentaenoic (20:5 ω-3), FOS, carnitine and mixtures thereof. HMB may be added to food products comprising a source of amino-nitrogen enriched with large neutral amino acids such as leucine, isoleucine, valine, tyrosine, threonine and phenylalanine and substantially lacking in free amino acids.

Anti-sclerostin antibodies are formulated as lyophilisates. The lyophilisates can be reconstituted to give a solution with a high concentration of the antibody active ingredient for delivery to a patient without high levels of antibody aggregation. The lyophilisate can be reconstituted with an aqueous reconstituent to provide an aqueous composition in which the antibody has a concentration of at least 25 mg / ml. The lyophilisate may include one or more of a sugar, a buffering agent, a surfactant, and / or a free amino acid.

The invention discloses a sea cucumber polypeptide functional food and a preparation method thereof which not only remarkably improves the content of polypeptide, but also has no chemical residues; the contents of salt and arsenic are low; the food is healthier and safer to eat; simultaneously the food is simply operated, easily controlled, is effective and saves energies. The key technical scheme includes: selecting a compound protease of Protamex and needing not to adjust the pH value of materials; carrying out processes of desalting and arsenic removing on an enzymolysis liquid; more than 80 percent of the molecular weight of the product is between 100 to 6000Dalt; wherein, the small polypeptide of 100 to 2100Dalt is more than 70 percent; the product components and the content weight percentages are as follows: 50 to 60 percent of polypeptide, 5 to 10 percent of free amino acids, 2.5 to 7.5 percent of mucoitin as well as containing the inherent nutrition components of a plurality of minerals and vitamins of the sea cucumber. The product has the effects of resisting knub, reducing blood pressure, preventing cardio-cerebrovascular diseases, resisting fatigue, delaying senescence, improving the immunity. The food can be used as a healthy food to eat and can also be used as a food and a medicine additive.

The invention relates to a preparation method of a compound amino acid cheated nano-selenium leaf fertilizer. The preparation method comprises the following steps: (1) performing enzymatic hydrolysis on blood cell protein powder by using compound enzyme; (2) adding 3wt%-5wt% of sugar alcohol into enzymatic hydrolysis liquid, and reacting at 50-70 DEG C for 1-2 hours to obtain reaction liquid; (3) adding nano-selenium, and reacting at 45-65 DEG C for 1-2 hours to obtain initial liquid; (4) adding 5wt%-6wt% of nano titanium dioxide into the initial liquid, reacting at 45-55 DEG C for 0.5-1 hours, heating to 90-95 DEG C, and keeping the temperature for 10-20 minutes to form a final product. The leaf fertilizer prepared by adopting the method is rich in high-activity oligopeptide and free amino acid, easy to absorb by crops and quick to transport in the crops, and selenium and sugar alcohol can be quickly and completely dissolved; the problem of difficult absorption of the leaf fertilizer is solved, the growth speed of the crops is greatly increased, the disease resistance of the crops is greatly improved, and the quality of the crops is improved.

The invention discloses a multifunctional composite adsorbent. Cross-linked chitosan is used as a matrix and is formed by cross-linking heavy metal ion imprinting chitosan by using a cross-linking agent; the cross-linked chitosan matrix is filled with nano Fe3O4 and nano TiO2; and the surface of the multifunctional composite adsorbent is modified by thiocarbamide so as to form a large amount of free amino groups. The invention correspondingly discloses a preparation method for the multifunctional composite adsorbent. The preparation method comprises the following steps of: performing photocatalysis loading, performing heavy metal ion imprinting to ensure the metal imprinting ions are fully reacted with chitosan in the heavy metal imprinting ions to form complexes, and adding the cross-linking agent and reacting; and adding epichlorohydrin and an acetone solution containing thiocarbamide, and modifying and grafting, finally oscillating and desorbing, and regenerating by using an alkaline solution to prepare the multifunctional composite adsorbent. By using the multifunctional composite adsorbent, heavy metal can be selectively separated and / or organic pollutants can be degraded.

The present invention provides a functional probiotic fermented fruit and vegetable powder preparation method, a functional probiotic fermented fruit and vegetable powder product is prepared by low temperature spray drying method, and the advantages are in that: (1) vitamin and dietary fiber and other nutritional components in raw fruits and vegetables can be effectively saved, after fermentation, free amino acid and organic acid contents are increased greatly, the product not only has good nutrition, color and flavor, and good water solubility, is free of any flavor and pigments, and belongs to pure natural green food; (2) the activity of probiotics in the product can be effectively saved (live bacteria number is 106cfu / g-1010cfu / g), a large number of active probiotics have the special beneficial functions of regulating the human body intestinal environment, preventing intestinal inflammation and cancer, improving constipation and other functions, and common fruit and vegetable powder does not have the special beneficial functions; (3) the preparation method has the advantages of low processing equipment investment, high efficiency and low energy consumption, the cost can be reduced by more than 80%, the production is easy to enlarge, and the preparation method is suitable for industrial continuous production, and can truly meet the needs of ordinary consumers.

The invention discloses a cosmetic containing oceanic shellfish active peptide and a preparation method and application thereof. The cosmetic contains 0.1 to 10 percent of oceanic shellfish active peptide, or 0.1 to 10 percent of oceanic shellfish active peptide and 1 to 10 percent of hyaluronic acid, or 0.1 to 10 percent of oceanic shellfish active peptide, 1 to 10 percent of hyaluronic acid and1 to 25 percent of vitamin C (VC), and the balance is cosmetic matrix components. The peptide is formed by boiling, ultrasonic, enzymolysis and ultra-filtration of oceanic shellfish meat; and besidesthe active peptide, the oceanic shellfish meat also contains free amino acid, glycosaminoglycan, polyunsaturated fatty acid, vitamins, trace elements and the like. The problems of application and thelike of the oceanic shellfish active peptide in the cosmetic are solved; and the prepared cream, emulsion, aqueous agent, gel, aerosol or mask has more complete and remarkable skin-care or hair-care effect. After the hyaluronic acid and the VC are added, the effects of preserving moisture, increasing the elasticity and the smoothness of the skin, removing flaws and improving the skin color are remarkably enhanced.

Antibody formulations are described comprising a mixture of an anti-α4β7 antibody, an antioxidant or chelator, and at least one free amino acid. The disclosed formulations may have improved stability, reduced aggregate formation, or both. The present invention further provides a safe dosing regimen of these antibody formulations that is easy to follow, and which results in a therapeutically effective amount of the anti-α4β7 antibody in vivo.

The invention relates to a Dendrobium officinale mask and its preparation method. Nowadays, there is no Dendrobium officinale mask, in which active ingredients in Dendrobium officinale can be retained, or a preparation method thereof. The Dendrobium officinale mask contains 5-8 parts by weight of a filtered Dendrobium officinale extract which contains polysaccharide, chlorophyll, vitamin and protein. The preparation method of the Dendrobium officinale mask comprises the following processes in order: A, pretreatment process; B, low-temperature ultramicro-grinding process: placing water of 2-10 DEG C and cut-off Dendrobium officinale into a homogenizer with the weight ratio of Dendrobium officinale to water being 1:6-1:8, starting the homogenizer and controlling discharging material fineness to below 200-300 microns so as to obtain a Dendrobium officinale extract; C, filtration process; and D, emulsification process. According to the invention, a Dendrobium officinale fresh product used as a raw material undergoes low-temperature extraction, and active ingredients in Dendrobium officinale can be retained as much as possible. The contents of polysaccharide, vitamin, chlorophyll, free amino acid and the like in the product obtained are high.

The invention provides a magnetic nano-particle immobilized basic protease, the magnetic nano-particle immobilized basic protease is obtained by utilizing a silylating reagent of 3-aminopropyl-triethoxysilane to modify Fe3O4 nano-particles so as to improve water solubility and stability of the Fe3O4 nano-particles, introducing a free amino terminal onto the surface of the Fe3O4 nano-particles, and then using a bifunctional reagent of p-phenylene diisothiocyanate to fix the basic protease into the Fe3O4 nano-particles modified by the silylating reagent, and the magnetic nano-particles with surface functionalization are taken as a vector for immobilizing the basic protease so as to be applied in green transformation of biomass. The preparation method is simple and easy to operate, and a biological catalyst can be rapidly and conveniently recycled through an externally applied magnetic field, thereby being conductive to realization of continuous large-scale production through biological catalytic reaction.

The present invention relates to a method for preparing soybean peptides through enzymolysis of soy protein, wherein the obtained soybean peptides have characteristics of low bitter taste and high yield, and the contents of free amino acids and the peptides having a molecular weight of less than 150 Da or greater than 2000 Da are low. The method comprises: adding water to soybean protein to prepare a 4-12 wt% soybean separation protein liquid, adjusting the temperature and the pH value, sequentially adding a proper amount of alkaline proteinase adopted as the endopeptidase, neutral proteinase and flavourzyme adopted as the exopeptidase, carrying out an enzymolysis reaction, adjusting the pH value of the enzymolysis liquid, carrying out enzyme inactivation, centrifugating, taking the supernatant, sterilizing, and carrying out spray drying to obtain the soybean peptide.

The invention relates to a composition comprising carbohydrate and peptide material as well as an amount of at least one additional free amino acid selected from the group consisting of leucine and phenylalanine. This composition will enhance the blood insulin response after oral intake by humans and is intended for an enhanced recovery air physical exercise or to delay exhaustion during physical exercise.

Fermented soybean foods rich in gamma-aminobutyric acid are produced by fermentation of soybean using Tempe molds. Fermented soybean foods rich in gamma-aminobutyric acid and free amino acids are produced by fermentation of soybean using Tempe molds with anaerobic treatment. Fermented cereal foods rich in gamma-aminobutyric acid and free amino acids are also produced by fermentation of cereals with anaerobic treatment. Rhizopus oligosporus and Rhizopus oryzae are favorably used as the Tempe mold and Koji molds.

The invention discloses a simple-to-operate production method of sea cucumber peptide extract which contains high content of sea cucumber peptides and free amino acid nitrogen, is light-colored and is free of fishy smell. The production method comprises the steps of sea cucumber pretreatment, crushing, homogenization, enzyme hydrolysis, enzyme inactivation, filtration, decoloration and fishy smell removal. The production method is characterized in that the enzyme hydrolysis comprises the steps of regulating the pH of sea cucumber homogenate to 6.0-7.5, adding 1600-3100U / g hay bacillus neutral proteases and 250-700U / g flavourzyme into the sea cucumber homogenate, conducting enzyme hydrolysis for 2-5h at 50-65 DEG C; the enzyme inactivation comprises the step of heating for 10 minutes at 100 DEG C; and the decoloration and fishy smell removal comprise the step of conducting decoloration and fishy smell removal to enzymatic solution by using anion resin.

The invention discloses a method for producing low-salt fish sauce, which comprises the following steps of: adding crushed prawn heads into a crushed low-value fish table salt solution; carrying out enzymolysis fermentation at low temperature; and after fermentation and maturation, removing protein and other impurities with incomplete fermentation by steps of filtering, heating for sterilizing, refiltering, and the like. Because the endogenic protease of the prawn heads is used for carrying out enzymolysis fermentation, the invention not only can improve the enzymolysis speed of the fish protein and shorten the fermenting period at low temperature but also can inhibit the breeding of rotten microorganisms in the fermenting process at the low temperature; in the invention, free amino acid generated by fish protein hydrolysis can prompt fermentation liquor pH to decrease in the low-temperature enzymolysis process so as to further inhibit the breeding of the rotten microorganisms; and when the pH is reduced to a certain degree, the fermenting temperature can be also improved to further shorten fermenting time, and the fermented and matured fish sauce has less fishy smell compared with the traditional fermented fish sauce.

The invention relates to a method for preparing a germinated brown rice ferment drink. Germinated brown rice is processed in an Aspergillus oryzae and Rhizopus oryzae mixed culture manner, is decomposed by using the enzyme system complementarity, and is inoculated with lactic acid bacteria to carry out fermentation. The prepared germinated brown rice ferment drink is rich in organic acids, variousfree amino acids, soluble dietary fibers, and active components which are gamma-aminobutyric acid and SOD, and has the advantages of sour-sweet mouthfeel, convenience in drinking, no additives, excellent nutrition and healthcare effects, great improvement of the raw material utilization rate in a two-stage fermentation process, and great solving of resource wastes of existing brown rice having bad mouthfeel.

Anti-CD40 antibodies are formulated as lyophilisate or liquid formulation. The lyophilisates can be reconstituted to give a solution with a high concentration of the antibody active ingredient for delivery to a patient without high levels of antibody aggregation. The lyophilisate can be reconstituted with an aqueous reconstituent to provide an aqueous composition in which the antibody has a concentration of at least 50 mg / ml. The lyophilisate or aqueous pharmaceutical composition may include one or more of a sugar, a buffering agent, a surfactant, and / or a free amino acid.