484 results about "Free amino" patented technology

Antibody formulations are described comprising a mixture of an anti-α4β7 antibody, an antioxidant or chelator, and at least one free amino acid. The disclosed formulations may have improved stability, reduced aggregate formation, or both. The present invention further provides a safe dosing regimen of these antibody formulations that is easy to follow, and which results in a therapeutically effective amount of the anti-α4β7 antibody in vivo.

The invention discloses cured beef and a preparation method thereof, and belongs to the technical field of fermented food. Comprising the following steps: trimming and dicing beef, adding seasoning spices, and adding a microbial agent for fermentation, the microbial agent is prepared from lactobacillus plantarum NR1-7 and aroma-producing yeast. According to the invention, the cured beef fermented by adding the Chinese prickly ash extracting solution, the lactobacillus plantarum NR1-7 and the aroma-producing yeast is relatively high in viable count, good in color and luster, rich in free amino acid quantity, moderate in protease activity and appropriate in organic acid content, and the pH value is stabilized to be about 5.5. Compared with natural fermentation, the growth and reproduction of inoculated dominant bacteria can accelerate the degradation of beef components and generate rich flavor, the pepper extract can ensure the complete beef structure by weakening the activity of endogenous enzyme, and in addition, the sour taste can be alleviated, the excessive degradation of fat can be relieved, and the fermentation quality of the cured beef can be improved.

Antibody formulations are described comprising a mixture of an anti-α4β7 antibody, an antioxidant or chelator, and at least one free amino acid. The disclosed formulations may have improved stability, reduced aggregate formation, or both. The present invention further provides a safe dosing regimen of these antibody formulations that is easy to follow, and which results in a therapeutically effective amount of the anti-α4β7 antibody in vivo.

The invention relates to the technical field of daily chemicals, in particular to a hair repairing agent and application thereof. The shampoo is prepared from the following raw materials: a suspending agent, a surfactant, a hair conditioner, a solvent, a cleaning agent, a functional repairing agent, a preservative, an aromatic, a thickening agent and a humectant. Fibroin is degummed through bifidus yeast by adopting a biological fermentation technology to obtain a protein active matter with lower molecular weight, a fibroin derivative is rich in a large amount of amino acid, glycine, alanine and serine account for about 85% of the total composition, the fibroin derivative has very strong moisturizing performance, the extract can rapidly permeate into hair cores, and the effect of moisturizing the hair cores is achieved. The water bonding capability of hair is enhanced, naturally existing free amino acids and lost polypeptides are supplemented, the fiber structure of the hair is recombined, the strength, toughness and glossiness of the hair are enhanced, and branching and embrittlement are prevented.

Provided herein are methods for treating a pulmonary infection in a patient in need thereof, for example, a nontuberculous mycobacterial pulmonary infection for at least one treatment cycle. The method comprises administering to the lungs of the patient a pharmaceutical composition comprising a liposomal complexed aminoglycoside comprising a lipid component comprising electrically neutral lipids and an aminoglycoside. Administration comprises aerosolizing the pharmaceutical composition to provide an aerosolized pharmaceutical composition comprising a mixture of free aminoglycoside and liposomal complexed aminoglycoside, and administering the aerosolized pharmaceutical composition via a nebulizer to the lungs of the patient. The methods provided herein result in a change from baseline on the semi-quantitative scale for mycobacterial culture for a treated patient, and / or NTM culture conversion to negative during or after the administration period.

Provided herein are methods for treating a pulmonary infection in a patient in need thereof, for example, a nontuberculous mycobacterial pulmonary infection for at least one treatment cycle. The method comprises administering to the lungs of the patient a pharmaceutical composition comprising a liposomal complexed aminoglycoside comprising a lipid component comprising electrically neutral lipids and an aminoglycoside. Administration comprises aerosolizing the pharmaceutical composition to provide an aerosolized pharmaceutical composition comprising a mixture of free aminoglycoside and liposomal complexed aminoglycoside, and administering the aerosolized pharmaceutical composition via a nebulizer to the lungs of the patient. The methods provided herein result in a change from baseline on the semi-quantitative scale for mycobacterial culture for a treated patient, and / or NTM culture conversion to negative during or after the administration period.

The application discloses an Aspergillus niger strain and a culture method and application thereof, the Aspergillus niger strain is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20241727. The fermented tea product obtained by fermenting tea leaves or tea leaf extracts by using the Aspergillus niger strain CCTCC NO: M 20241727 has prominent sweet and floral fragrance, and the astringency is obviously reduced; the contents of tea polyphenols, free amino acids and flavones are higher than those of a fermented tea product of an existing Aspergillus niger strain; the antioxidant activity is enhanced; the tea soup color is orange red and bright; and the color, fragrance and taste qualities are all superior to those of the fermented tea product of the existing Aspergillus niger strain.

The invention discloses Kluffa yeast and application of the Kluffa yeast in preparation of red camellia fermentation liquor with anti-aging and repairing functions, the Kluffa yeast is used for repairing PR-LQX002, the Latin name of the Kluffa yeast is Lachancea kluyveri, and the preservation number of the Kluffa yeast is CCTCC NO: M 20241723. The invention also provides a red camellia fermentation broth, a composition containing the fermentation broth and application of the red camellia fermentation broth in preparation of products with anti-oxidation, anti-saccharification, anti-wrinkle or repair effects. The fermentation broth is prepared from the following raw materials: red camellia flowers, red camellia leaves and Kluffa yeast PR-LQX002. The Kluffa yeast can greatly promote fermentation of flowers and leaves of the red camellia, and the obtained red camellia fermentation liquor not only contains active substances such as flavone, polyphenol and free amino acid, but also contains lipid substances such as ceramide, and has multiple effects such as saccharification resistance, repair, wrinkle resistance and oxidation resistance.

The invention relates to the technical field of protective films, in particular to a high-temperature-resistant low-viscosity adhesive-residue-free protective film and a preparation method thereof. In order to improve the adhesive residue-free performance of the protective film, 4-carboxyphenylboronic acid pinacol ester and bis (4-aminophenoxy) dimethylsilane are used as raw materials, and a boron-silicon intermediate containing free amino groups is prepared by controlling the change of reaction conditions and addition amount; wherein the boron element and the silicon element have good flame retardant property, the flame retardant property of the protective film can be effectively improved, the safety coefficient in the processing process is improved, and due to the introduction of the boron element and the silicon element, a-O-B-O-chain segment and a-O-Si-O-chain segment are introduced into the adhesive and have high bond energy, so that the flame retardant property of the protective film is improved. The high-temperature stability of the adhesive can be effectively improved, so that the adhesive still has excellent performance at high temperature, the cohesion of the adhesive can be effectively improved, and the adhesive residue phenomenon in the process of removing the protective film is avoided.

The invention relates to the technical field of agricultural fertilizer preparation, and discloses a preparation method of a water-soluble fertilizer rich in various active amino acids, which comprises the following steps: S1, preparing an amino acid solution: dissolving various free amino acids or polypeptides in a buffer solution to form an amino acid precursor liquid; s2, constructing a nutrition core layer: adding one or more types of soluble trace element salts and a gelation auxiliary agent into the amino acid precursor liquid to form gelatinous particles; s3, forming an intermediate response layer: coating the core particles with chitosan and phospholipid to form a bimolecular membrane structure. The stability of the particle structure is improved through low-temperature crosslinking and bi-component gelation, and quick response and continuous controlled release in a weak acid environment are realized by combining an intermediate response membrane and an outer controlled release shell layer; by adopting a structure-function integrated design, the suspension property and the dispersity are remarkably improved, and the multi-crop and multi-period precise fertilization requirements are met.

The invention belongs to the technical field of immunodetection, and discloses a kit for detecting the ratio of proBDNF to maure BDNF based on structural dynamics guidance and a preparation method of the kit, the kit comprises a solid phase carrier and a detection reagent, the three antibodies are determined on the basis of full-length conformational kinetics analysis of brain-derived neurotrophic factors; the first capture antibody targets an innate disorder region (SEQ ID NO: 1) of a proBDNF propeptide region; the second capture antibody targets an innate disorder region (SEQ ID NO: 2) of a proBDNF propeptide region; the second capture antibody is a neo-epitope specific antibody, is specifically combined with an N terminal (SEQ ID NO: 2) exposed by maure BDNF enzyme digestion, and is combined with a free alpha-amino group strictly dependent on the first histidine; and the universal detection antibody is combined to a Loop 4 region (SEQ ID NO: 3) of the Mature structural domain. The problems of steric hindrance and cross reaction in traditional immunodetection are solved through a structural biology strategy, and accurate distinguishing and ratio quantification of proBDNF and maure BDNF are achieved.

Provided herein are methods for treating a pulmonary infection in a patient in need thereof, for example, a nontuberculous mycobacterial pulmonary infection for at least one treatment cycle. The method comprises administering to the lungs of the patient a pharmaceutical composition comprising a liposomal complexed aminoglycoside comprising a lipid component comprising electrically neutral lipids and an aminoglycoside. Administration comprises aerosolizing the pharmaceutical composition to provide an aerosolized pharmaceutical composition comprising a mixture of free aminoglycoside and liposomal complexed aminoglycoside, and administering the aerosolized pharmaceutical composition via a nebulizer to the lungs of the patient. The methods provided herein result in a change from baseline on the semi-quantitative scale for mycobacterial culture for a treated patient, and / or NTM culture conversion to negative during or after the administration period.

The invention provides a method for detecting free amino acids in camellia oleosa seed oil, and establishes a method for detecting the free amino acids in the camellia oleosa seed oil based on ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS), which comprises the following steps: pre-treating various amino acids in the camellia oleosa seed oil by using a non-derivation method; the problems of complicated operation, impurity interference and the like caused by derivatization are avoided, the extraction solvent is environment-friendly and economical, the extraction efficiency is high, and the pretreatment time is greatly shortened; then selecting a proper chromatographic column and a mobile phase, separating a plurality of free amino acids under the condition of not introducing excessive derivatization agents and ion pair reagents, and controlling the collection time to be 12 minutes. The method for detecting the free amino acids in the camellia oleosa seed oil has the characteristics of no need of derivatization treatment, simplicity and convenience in operation, high detection efficiency and good sensitivity and accuracy, and can effectively separate and detect 14 free amino acids such as alanine, phenylalanine and aspartic acid in the camellia oleosa seed oil.

The invention discloses a high-yield protease salt denitration mycobacillus for improving the quality of low-salt shrimp paste, and belongs to the field of food biotechnology and application. The salt denitration mycobacillus XJ-229 provided by the invention has relatively good salt tolerance (the tolerance range is 1%-22%) and enzyme production (the protease production is 15.38 U / mL) capacity, and a proper fermentation agent is provided for fermentation of low-salt shrimp paste. The cladobacillus sp. XJ-229 is used for preparing shrimp paste, the content of volatile basic nitrogen, malondialdehyde and biogenic amine can be reduced, the content of free amino acid can be increased, the flavor of the shrimp paste can be improved, and the prepared shrimp paste can be accepted by people more easily. Therefore, the salt denitration mycobacillus XJ-229 provided by the invention is beneficial to improving the quality of the shrimp paste and improving the market acceptability of the shrimp paste.

The invention belongs to the technical field of food processing, and particularly relates to fermented beef jerky and a preparation method thereof. The preparation method of the beef jerky comprises the following steps: (1) removing fascia and fat from fresh cattle hind leg meat, washing with clear water, and cutting into meat slices; (2) adding table salt, glucose, white granulated sugar and sodium nitrite into the sliced meat, uniformly mixing, adding a fermentation inoculant which comprises staphylococcus calcaratus, pediococcus acidilactici, bifidobacterium bifidum and Witzia ciliaris, and fermenting; and (3) after fermentation, drying and cooling to obtain the fermented beef jerky. Specific strains are selected to form a fermentation inoculant for fermentation, and the prepared fermented beef jerky is high in free amino acid and soluble peptide content, low in nitrite residual quantity, uniform red brown in color and slightly bright in surface, and belongs to high-quality beef jerky.

The invention discloses an anti-aging composition derived from space breeding desert algae as well as a preparation method and application of the anti-aging composition, and relates to the technical field of application of desert algae. The anti-aging composition is an extract of Hassalliasp.P06 after space breeding, and the preservation number of the Hassalliasp.P06 is CCTCC (China Center for Type Culture Collection) M20242054, and the preservation number of the Hassalliasp.P06 is CCTCC M20242054. The anti-aging composition derived from the space breeding desert algae comprises the following components: polypeptide, free amino acid, polysaccharide and cyclic adenosine monophosphate. The skin care product has very strong moisturizing capacity, can promote the proliferation of skin fibroblasts and remarkably improve the skin compactness, has a soothing effect and oxidation resistance, and can be applied to skin care products or cosmetics to be used as a compactness agent or an anti-aging agent so as to improve the skin aging symptom.

The invention relates to the technical field of agricultural fertilizers, and discloses a preparation method of an amino acid foliar fertilizer extracted based on animal hair, and the preparation method comprises the following steps: raw material pretreatment: carrying out plasma modification on animal hair to improve the keratin hydrolysis performance; a double hydrolysis process: firstly decomposing a keratin structure in the hair through sulfuric acid hydrolysis, and then generating free amino acid through enzyme hydrolysis; recycling by-products: preparing biochar or protein feed from undissolved residues generated in the hydrolysis process, and separating and recycling amino acids and salts in waste liquid; and preparing a foliar fertilizer formula: chelating the extracted amino acid concentrated solution with trace elements. The technical effects of zero emission and high-valued conversion of wastes are achieved through a resource utilization scheme that biochar is prepared from undissolved residues through a pyrolysis process or protein feed is prepared from the undissolved residues through secondary enzymolysis, and free amino acids and salts in waste liquid are separated and recycled through a membrane separation and crystallization extraction technology.

The present invention belongs to the field of cell culture media. Provided are a method for preparing a stem cell culture medium by using a protein hydrolysate as a core raw material and a method of using the stem cell culture medium. The stem cell culture medium contains 20-80% of the protein hydrolysate on the basis of the total weight of the components in the stem cell culture medium. The protein hydrolysate comprises one or more of a high-free amino acid yeast hydrolysate, a high-nucleotide yeast hydrolysate, a high-glutathione yeast hydrolysate, a high-polypeptide yeast hydrolysate, a rice protein hydrolysate, a high-glutamine wheat protein hydrolysate, a pea protein hydrolysate and a soybean protein hydrolysate. The preparation of a natural animal stem cell culture medium by using a whole nutrient protein hydrolysate as a core raw material can meet the nutritional requirements for animal stem cell proliferation under the conditions of no additional addition or addition of a small amount of amino acids, vitamins, growth factors and serum, which effectively reduces the cost of the stem cell culture medium, thereby providing the stem cell culture medium with wide application prospects in the field of foods such as cultured meat.

The invention relates to the technical field of organic fertilizers, in particular to an alginic acid organic fertilizer and a preparation method thereof.The alginic acid organic fertilizer is prepared from 450-550 g / L of seaweed extract, 80-110 g / L of free amino acid, 80-110 g / L of nitrogen (N), 80-110 g / L of phosphorus (P2O5), 80-110 g / L of potassium (K2O), 40-60 g / L of microbial agent and microelements, and the alginic acid organic fertilizer can effectively improve the yield and quality of strawberries. According to the preparation method, complex instruments and equipment and special reaction conditions are not needed in the preparation process of the alginic acid organic fertilizer, large-scale production is easy, the production cost is reduced, the production efficiency is improved, and popularization and application of the product in the market are facilitated.

The invention belongs to the technical field of corn peptide production, and particularly relates to a corn peptide product with a flavoring function as well as a preparation method and application thereof. The content of peptides with the molecular weight of 189-10000 Da in the corn peptide product is 40-60%. The total nitrogen content of the corn peptide product is 8.0-14.0%, and / or the free amino acid content is 10-20%, and / or the free glutamic acid content is 2.0-7.0 wt%. The corn peptide product is prepared by the following preparation method: carrying out enzymolysis on a mixed solution containing crude corn protein powder and / or corn flour through amylase, phospholipase, lipase, cellulase and protease to obtain the corn peptide product with a flavoring function. The corn peptide product has the characteristics of low bitterness value, delicate flavor, good thick flavor and full taste, and can be widely applied to the field of condiments.

The invention discloses an amino acid water-soluble fertilizer and a production process thereof, and relates to the technical field of water-soluble fertilizers. Free amino acid is used as an organic nitrogen source, and the medium element calcium is combined, so that the crop quality is improved. The formula is low in cost, high in absorptivity, suitable for crop growth, simple and convenient to use and high in cost performance, and promotes the synergistic effect of nutrient elements. The method takes amino acid and calcium as raw materials, and has the characteristics of wide applicability, high output-input ratio and easiness in market promotion. The product is completely water-soluble, environment-friendly and safe, can regulate the physiological function of crops, increase the yield and amino acid content and improve the quality, and has remarkable economic benefits. In addition, activated ricinoleic acid carboxyl is combined with fucoidan enzymatic hydrolysis oligosaccharide to construct the microcapsule, so that efficient utilization of resources is realized.

The invention provides a method for preparing a compound amino acid chelated slow-release fertilizer from waste spirulina mud, and belongs to the technical field of agricultural fertilizers. According to the method disclosed by the invention, the waste spirulina mud from which phycocyanin and polysaccharide are extracted is fully utilized, the waste spirulina mud is decomposed into free amino acid, and the free amino acid is compounded with marine polysaccharide to prepare a green compound amino acid chelated fertilizer product with a slow-release effect; secondly, the nutrient release period of the fertilizer can be regulated and controlled by controlling the proportion and concentration of the marine polysaccharide, and the fertilizer has excellent water and fertilizer retention capacity.

The invention belongs to the technical field of microorganisms, and particularly discloses a strain of Paenibacillus amyloliquefaciens (Paenibacillus amyloliquefaciens) capable of increasing the yield of tea leaves and improving the quality of the tea leaves and an application of the Paenibacillus amyloliquefaciens (Paenibacillus amyloliquefaciens) capable of increasing the yield of the tea leaves and improving the quality of the tea leaves. The preservation number of the chitin-dissolving paenibacillus is CCTCC NO: M 20241893, and the strain has efficient potassium-dissolving activity, strong organophosphorus-dissolving activity and IAA (Indoleacetic Acid) production capacity, and can degrade chitin by secreting chitinase, so that the strain has certain inhibition capacity on fungal pathogenic bacteria, and can be applied to the field of agricultural production. The bacterial strain can increase the content of rapidly available phosphorus and rapidly available potassium in tea garden soil, increase the total potassium content of tea leaves, increase the density of tea buds and the yield of the tea leaves, increase the content of free amino acids in the tea leaves and reduce the content of tea polyphenols and the phenol-ammonia ratio of the tea leaves, so that the quality of the tea leaves is improved.

The invention relates to the technical field of chemical analysis and detection, in particular to a fluorescence determination method for free amino acid in tobacco leaves, based on the fluorescence characteristic of epinephrine-based zinc-doped carbon dots Zn / NA-CDs, free amino acid reacts with phthalic dicarboxaldehyde to generate a Schiff base product, and Zn / NA-CDs and Schiff base have hydrogen bond interaction and charge transfer effect, so that the content of free amino acid in tobacco leaves is determined. Therefore, the Schiff base has a fluorescence sensitization effect on the Zn / NA-CDs, and a free amino acid fluorescence detection method is established. According to the method, the detection limit can reach 0.01 mg / L, and the recovery rate is between 90.52% and 105.33%, so that the method also has high sensitivity to low-concentration free amino acid, can meet the requirement for accurately measuring trace amino acid in tobacco leaves, and has excellent accuracy and reliability. Meanwhile, the method further has the advantages of being easy and convenient to operate, low in cost, environmentally friendly, wide in applicability and the like, a new technical means is provided for tobacco leaf quality detection, and beneficial reference is provided for amino acid determination in other fields.

Provided herein are methods for treating a pulmonary infection in a patient in need thereof, for example, a nontuberculous mycobacterial pulmonary infection for at least one treatment cycle. The method comprises administering to the lungs of the patient a pharmaceutical composition comprising a liposomal complexed aminoglycoside comprising a lipid component comprising electrically neutral lipids and an aminoglycoside. Administration comprises aerosolizing the pharmaceutical composition to provide an aerosolized pharmaceutical composition comprising a mixture of free aminoglycoside and liposomal complexed aminoglycoside, and administering the aerosolized pharmaceutical composition via a nebulizer to the lungs of the patient. The methods provided herein result in a change from baseline on the semi-quantitative scale for mycobacterial culture for a treated patient, and / or NTM culture conversion to negative during or after the administration period.

The invention relates to aspergillus oryzae sourced protease and application thereof in traditional bean fermentation products, and belongs to the technical field of enzyme engineering and microbial engineering. The amino acid sequence provided by the invention comprises an acid protease, two neutral proteases and an alkaline protease, wherein the proteases have higher degradation degree on globulin and albumin; the enzyme activity is the highest at 45-50 DEG C, and the salt tolerance is good. When the soybean sauce is prepared, the physicochemical indexes, free amino acids, organic acids and flavor substances of the soybean sauce are remarkably improved; wherein the quantity and the content of flavor substances are remarkably increased, and the content of free amino acid is increased by 20-25%; and the flavor sensory evaluation is more pleasant. In the early stage to the middle stage of fermentation, the content of amino acid nitrogen in the soy sauce is remarkably increased, the production cycle of the soy sauce can be shortened, and the method has wide application prospects in the food industry.

Methods for making wettable, dispersible potato protein powders are provided. The wettable dispersible potato protein powders comprise potato protein, free amino acids, non-protein nitrogen compounds, and minerals from potatoes; and are low in total α-glycoalkaloids. The inventive potato protein powders are suitable for use in formulating foods, feeds and beverages.

The invention discloses a production and processing method of an asparagus fermented beverage, and belongs to the technical field of food processing. The production and processing of the asparagus fermented beverage specifically comprises the following steps: cleaning fresh asparagus, cutting into sections, blanching, draining, cooling and pulping to obtain asparagus juice; adding citric acid, cellulase and pectinase, performing enzymolysis at 50-70 DEG C, and then performing enzyme deactivation to obtain enzymatic hydrolysate; and adding glucose, cane sugar and skimmed milk powder into the enzymatic hydrolysate, sterilizing, cooling, adding rhamnose lactobacillus and kluyveromyces marxianus, fermenting, centrifuging, and sterilizing, thereby obtaining the product. The asparagus fermented beverage is pleasant in flavor and high in free amino acid content, is particularly rich in gamma-aminobutyric acid, and has the effects of helping sleep, relaxing the bowels and the like.

The invention relates to the field of biology, in particular to a sheep waste compound bacterium fermented organic fertilizer which is obtained by fermenting compound bacteria, and the compound bacteria comprise any one of bacillus subtilis, lactic acid bacteria, saccharomyces cerevisiae and clostridium butyricum. The organic fertilizer has the following beneficial effects: (1) the organic fertilizer is fermented by using four microorganisms at the same time, so that the fermentation is more sufficient, and the content of free amino acid is relatively high; (2) the organic fertilizer contains four kinds of probiotics, can obviously inhibit diseases and insect pests, and actual planting of tomatoes and Chinese cabbages also proves this; and (3) the problems that the sheep slaughtering wastes pollute the environment and are discharged are solved, a high-quality organic fertilizer source can be provided for agricultural production, and the wastes are comprehensively recycled.

The invention discloses a method for controlling generation of acrylamide in a food processing process. The method is characterized by comprising the following steps: S1, reducing the content of free amino acid and reducing sugar in raw materials; s2, changing the processing conditions and the processing technology; and S3, using an inhibitor. By adopting the control method disclosed by the invention, the generation of acrylamide in the food processing process can be effectively inhibited, so that the food safety is greatly improved.