50 results about "Free amino" patented technology

The application discloses a composition, which comprises hydrolyzed protein; the hydrolyzed protein comprises hydrolyzed whey protein and hydrolyzed casein protein, the weight ratio of the hydrolyzed whey protein and the hydrolyzed casein protein is (70-95):(5-30), the amino acid nitrogen content in the hydrolyzed protein is (1.0-3.0) g / 100 g, the proportion of protein hydrolysate with a relative molecular mass distribution <1000 in the hydrolyzed protein is 34-70%, the peptide content (calculated on a dry basis) in the hydrolyzed protein is not less than 33 g / 100 g, and the total free amino acid content in the hydrolyzed protein is not less than 1 g / 100 g. The composition can effectively treat diarrhea and is suitable for people with gastrointestinal dysfunction and severe patients with diarrhea symptoms.

Antibody formulations are described comprising a mixture of an anti-α4β7 antibody, an antioxidant or chelator, and at least one free amino acid. The disclosed formulations may have improved stability, reduced aggregate formation, or both. The present invention further provides a safe dosing regimen of these antibody formulations that is easy to follow, and which results in a therapeutically effective amount of the anti-α4β7 antibody in vivo.

The present invention relates to nutritional compositions comprising digestible carbohydrates, lipids, proteins, 3-fucosyl lactose (3-FL) and a butyrate source, which are suitable for providing nutrition to an infant or young child suffering from or at risk of developing a food allergy, preferably cow milk protein or egg allergy, wherein the protein is or consists essentially of hydrolysed protein and / or free amino acids, and wherein the butyrate is provided in the form of dietary butyrate and / or by non-digestible saccharides selected from non-digestible polysaccharides, non-digestible oligosaccharides or mixtures thereof capable of undergoing microbial fermentation in the gastrointestinal tract to produce butyrate, and uses thereof.

The application belongs to the technical field of cottonseed meal processing and animal feed, and particularly relates to application of enzymatic cottonseed meal in preparation of low-protein daily ration, promotion of animal intestinal development, improvement of animal production performance and improvement of carcass quality, wherein the small peptide content, free amino acid content and antioxidant activity of the enzymatic cottonseed meal are all significantly improved compared with cottonseed meal, the change of protein secondary structure and the significant reduction of relative molecular weight are more conducive to promotion of animal absorption of small molecules, are beneficial to animal intestinal health development, and can effectively improve animal growth performance. The feed prepared by replacing part of the conventional feed with the enzymatic cottonseed meal can still achieve the effects of promoting animal intestinal development and improving animal growth performance under the condition that the crude protein content is reduced by 1% to 2% compared with the basic daily ration or the conventional feed, and the feeding cost is significantly reduced compared with the use of the conventional feed.

The present application belongs to the technical field of fermentation engineering, and particularly relates to a sheep liver peptide composition and a preparation method thereof, which are helpful for tonifying qi and blood and relieving dysmenorrhea. The sheep liver peptide composition comprises the following components in mass fractions: sheep liver peptide powder 60-70 parts, red date powder 5-15 parts, ginger powder 3-8 parts, licorice powder 3-8 parts, rose powder 2-6 parts, inulin 5-10 parts, astragalus peptide 5-10 parts, polygonatum kingianum peptide 5-10 parts, pepper powder 1-3 parts, cassia powder 1-3 parts, turmeric powder 2-5 parts, vitamin C 0.5-2 parts, and vitamin E 0.5-2 parts. The sheep liver peptide powder prepared by the present application has high contents of protein, total amino acids, free amino acids and vitamin A. The sheep liver peptide composition prepared by compounding the sheep liver peptide powder with the other components has the effects of tonifying qi and blood and relieving dysmenorrhea.

A method for the precise cellular destruction of an oncogenic non-coding RNA with a RNA-binding small molecule conjugated with bleomycin A5 is described. The method affords reversal of phenotype. Bleomycin A5 was coupled to an RNA-binding molecule that selectively binds the microRNA-96 hairpin precursor (pri-miR-96). By coupling of bleomycin A5's free amine to the RNA-binding molecule, its affinity for binding to pri-miR-96 is >100-fold stronger than to DNA. The conjugate compound selectively cleaves pri-miR-96 in triple negative breast cancer (TNBC) cells. Selective cleavage of pri-miR-96 enhances expression of FOXO1 protein, a pro-apoptotic transcription factor that miR-96 silences, and triggers apoptosis in TNBC cells. No effects were observed in healthy breast epithelial cells. This method provides programmable control for targeting RNA through the selection of an RNA-binding molecule / bleomycin A5 conjugate and provides a facile method of mapping the cellular binding sites of an RNA-binding molecule.

This invention discloses a preparation process for antibacterial long-staple cotton towels, comprising: pre-treating long-staple cotton fibers with low-alkali scouring and oxygen bleaching; selectively oxidizing chitosan fibers to introduce aldehyde functional groups onto their surface, resulting in partially aldehyde-modified chitosan fibers; blending the partially aldehyde-modified chitosan fibers with the pre-treated long-staple cotton fibers and weaving them into towel fabric; after dyeing, finishing with a reactive antibacterial finishing agent containing free amino groups, whereby, under the action of a polycarboxylic acid crosslinking agent, the aldehyde groups on the surface of the oxidized chitosan fibers react with the amino groups in the finishing agent to form a Schiff base reaction, while the finishing agent and long-staple cotton fibers form a stable crosslinked structure; and then undergoing a reduction stabilization treatment to convert the Schiff base bonds into stable carbon-nitrogen single bonds. This invention achieves firm fixation of antibacterial components through a chemical anchoring mechanism, and the resulting towels maintain an antibacterial rate of no less than 95% after 50 standard washes, while also retaining good softness and absorbency.

The application relates to the technical field of food brewing processing, and discloses a method for preparing walnut soy sauce by using segmented wet heat pretreatment combined with multi-strain fermentation, which comprises the following steps: subjecting walnut meal to dry heat treatment, adjusting humidity, transferring into a high-temperature steam explosion equipment for steam explosion treatment and air cooling; inoculating the air-cooled walnut meal with mixed strains composed of aspergillus oryzae, saccharomyces rouxii and lactobacillus plantarum to prepare koji; adding brine to the koji and carrying out segmented fermentation including three stages to obtain soy sauce mash; adding oil to the soy sauce mash, precipitating the original solution, filtering the clear liquid, cooling the sterilized product to obtain a finished product. The present application combines wet heat pretreatment with multi-strain segmented fermentation, destroys the dense structure of the walnut meal, improves the substrate hydrolysis rate, meets the metabolic conditions suitable for different strains, reduces the substrate competition, shortens the fermentation period, and improves the content of free amino acids and ester substances.

The application discloses a method for reducing lincomycin B component in a lincomycin fermentation process, which comprises adding soybean protein hydrolysate A in the lincomycin fermentation process, so that the lincomycin B component can be effectively reduced, and the A component titer can be improved. The soybean protein hydrolysate A is a complex amino acid mixture obtained by enzymolysis of soybean raw materials, and the components include peptide segment A, free amino acid solution A, water or others, wherein the mass percentage of the peptide segment A is 30-35%, the mass percentage of the free amino acid solution A is 55-65%, and the water and the others account for 5-15%. After optimization, the production level of the B component in a 50 L fermenter is at least 166 mu g / mL, which is reduced by more than 75% compared with the yield of the B component in the original fermentation culture method, the product quality and the production efficiency are improved, and the method is suitable for industrialized scale production.

This invention discloses a method for preparing almond leaves fermented with *Lactobacillus plantarum*, the fermentation product, and its applications, belonging to the field of microbial fermentation and natural product processing technology. The preparation method includes: almond leaf pretreatment, pulping, substrate preparation, sterilization, inoculation with *Lactobacillus plantarum*, fermentation, inactivation, and separation and purification steps. This invention utilizes *Lactobacillus plantarum* for targeted biotransformation of almond leaves, resulting in a significant increase in the total polyphenol and total flavonoid content of the fermentation product, and the generation of a large number of new small-molecule active peptides, free amino acids, and organic acids. Compared with unfermented almond leaf water extract, the antioxidant activity, soothing and repairing activity, and antibacterial activity of the fermentation product of this invention are significantly enhanced. The process of this invention is green and environmentally friendly, and the obtained fermentation product can be widely used as an active ingredient in cosmetics, skin care products, antibacterial care products, and functional foods.

The application belongs to the technical field of plant molecular breeding, and provides a corn ZmAAP37 Gene in regulating corn agronomic traits and grain nitrogen accumulation, the application includes at least one of the following: (1) increase the nitrogen content of corn kernel; (2) increase the free amino acid content of corn kernel; (3) improve the aboveground biomass of corn plant; (4) increase the plant height of corn plant; (5) delay the leaf senescence of corn plant; (6) improve the nitrogen utilization rate of corn. The application shows that, ZmAAP37 By increasing the transport of amino acids in leaves to phloem, the nitrogen remobilization is promoted, and finally the grain nitrogen concentration is significantly increased without affecting the grain yield. The application provides ZmAAP37 The gene can provide new gene resources for nitrogen utilization of crops, especially corn, and has great application value in regulating nitrogen utilization of crops.

The present application relates to the technical field of livestock feed, and discloses a low-protein feed preparation method for laying hens based on net energy and mineral regulation, which comprises a basic daily ration, a natural intestinal health promoting mineral compound and a functional additive, the basic daily ration is set to a low net energy level, and the sodium-potassium-chlorine electrolyte balance value is strictly controlled, the compound lipid emulsified energy source microcapsule powder in the functional additive releases medium-chain fatty acids in the early stage of the intestinal tract, and the medium-chain fatty acids are absorbed synchronously with free amino acids to improve the utilization rate of crude protein and reduce the corruption of the posterior intestinal tract, activated montmorillonite and natural clinoptilolite are used to cooperatively regulate the osmotic pressure of the intestinal tract, reduce the incidence of watery feces and ammonia emission, L-cystine is crosslinked in the oviduct to form a disulfide bond network skeleton, the expansion effect of selenium-rich tourmaline powder on microvessels is combined, the compression strength of the eggshell and the selenium content of the yolk are effectively improved, and the present application significantly reduces the feed-to-egg ratio, improves the intestinal health and the quality of the end product.

The application discloses a dendrobium amino acid soy sauce and a preparation method and application thereof, and relates to the field of soy sauce brewing technology. The dendrobium amino acid soy sauce is prepared from dendrobium protocorm and / or dendrobium callus as raw materials through physical stress strengthening, step-by-step enzymolysis, starter preparation by mixing raw materials, low-salt solid-state segmented fermentation and aging. The physical stress strengthening includes light quality stress and / or low-temperature stress. The dendrobium protocorm and / or dendrobium callus are used as starting raw materials, and active ingredients such as free amino acids, gamma-aminobutyric acid, aspartic acid, dendrobium polysaccharide and alkaloids are directionally enriched through light quality stress, low-temperature stress or combined stress. The total amount of free amino acids is increased by 35% to 50% compared to ordinary natural dendrobium raw materials, the endogenous enrichment of functional ingredients is realized from the source, and there is no need to add artificial amino acid additives from the outside, so that the product is high in natural degree and good in safety.

The application discloses an Aspergillus oryzae strain A08 and application thereof, the Aspergillus oryzae A08 is preserved in the China General Microbiological Culture Collection Center on January 20, 2026, the suggested classification name is Aspergillus oryzae, and the preservation number is CGMCC No.42436.The koji-making process of the strain can simultaneously promote saccharifying enzyme and acid protease, the saccharifying enzyme activity is 1479 U / g, is increased by 31% than the original strain 3.042, the acid protease activity is also increased by 18.92% than the original strain 3.042, and raw material starch and protein are simultaneously degraded; the strain is used for high-salt dilute-state soy sauce fermentation, the D-anhydrous glucose yield is 2.51 times of the original strain, and the contents of free amino acid, sweet amino acid, glutamic acid and amino acid nitrogen are increased by 4.52%, 15.56%, 12.46% and 3.75% respectively.The application solves the problems of insufficient starch saccharification and insufficient protein degradation of the Aspergillus oryzae strain from the source, and has industrial application value.

This invention relates to the field of glucose preparation technology and relates to an unsaturated glucosamine with retained amino groups, its preparation method, and its applications. It solves the problems of low polymer drug loading, severe drug burst release, and poor biocompatibility caused by the blocking of amino groups in existing polymerizable monomers of glucosamine, as well as the cumbersome, low-yield, and poor-purity problems of existing methods for retaining amino groups. The preparation method includes: adding acetyl chloride to 3-buten-1-ol, stirring evenly at 0-5°C, then adding D-glucosamine hydrochloride in batches, heating to 60-80°C, and reacting for 2-4 hours. After the reaction is complete, post-treatment is performed to obtain an intermediate compound; dissolving the intermediate compound in deionized water, adding barium hydroxide hydrate, heating to 90-110°C, and stirring for 12-20 hours, after which post-treatment is performed to obtain unsaturated glucosamine with retained free amino groups. This invention can be applied to the preparation of drug carriers and has broad application prospects in the biomedical field.

The present application relates to the technical field of fermentation engineering and microbial culture medium matrix regulation, and discloses a compound culture medium for fermenting fish-source specific bacteria and a preparation method thereof, which comprises the following steps: adding sodium citrate to reduce the dynamic viscosity of fish-source initial materials, then hydrolyzing by using acid endoprotease, cross-flow filtering by using an ultrafiltration membrane assembly, and determining the transmembrane pressure difference to exclude the interference of polarization layer and determine the hydrolysis end point; hydrolyzing the ultrafiltration permeate under closed low-oxygen conditions by using neutral exopeptidase; and adding potassium dihydrogen phosphate and magnesium sulfate quantitatively to construct a steady-state coordination solvation structure. The present application blocks the excessive cleavage of polypeptides into free amino acids, limits the accumulation amount of free amino acids below the inhibition threshold, and eliminates the metabolic inhibition and fermentation amplification lag of specific bacteria in the downstream fermentation system of microorganisms.

This invention relates to the field of microbial technology, specifically to a strain of Aspergillus oryzae (… Aspergillus oryzae ZA305 and its applications. This invention uses Aspergillus oryzae strain Hu Niang 3.042 as the starting strain for ARTP mutagenesis. Through a multi-level directional screening method guided by flavor compounds, a strain of Aspergillus oryzae, ZA305, was obtained. This strain, when used in the fermentation of fermented black beans and broad bean paste, can significantly enhance the activity of key enzymes in the fermentation starter, increase the content of free amino acids for sweetness, soy sauce aroma, and fruit aroma key flavor substances in the product, and significantly reduce the content of undesirable flavor substances. It has a dual effect of enhancing aroma and suppressing defects, and can significantly improve the flavor, taste, and aroma harmony of fermented black beans and broad bean paste, significantly enhancing their soy sauce aroma, ester aroma, and umami flavor, and effectively reducing off-flavors, thereby comprehensively improving the quality of the product.

This invention discloses a brewer's yeast fermentation product solution and its application in cosmetics. The brewer's yeast fermentation product solution is prepared using modern fermentation technology, employing soybean milk, buckwheat sprouts, rice bran, algae, etc., as a composite fermentation substrate. Utilizing the metabolic activity of ordinary yeast, combined with subsequent yeast autolysis / enzymatic hydrolysis, a brewer's yeast fermentation product solution rich in α-ketoglutarate, polysaccharides, oligosaccharides, free amino acids, polypeptides, and B vitamins is prepared. This brewer's yeast fermentation product solution possesses moisturizing, repairing, and anti-aging properties and can be applied to creams, lotions, and other cosmetics, thereby endowing these cosmetics with hydrating, moisturizing, sensitive skin repair, and anti-aging effects.

The application discloses an oat extract and a preparation method and application thereof, and belongs to the technical field of functional peptide preparation.The oat extract provided by the application contains rich active peptides and free amino acids, and also contains vitamin E, linoleic acid and melatonin; on one hand, the melatonin, the active peptides and the tryptophan in the components directly improve sleep quality; on the other hand, the components reduce the oxidation level of the body, reduce the oxidation damage of nerve cells, provide sufficient raw materials to promote the repair and regeneration of the nerve cells, and strengthen the effect of improving sleep quality; in addition, the oat extract has the advantages of high safety and good flavor.

This invention belongs to the fields of medicinal chemistry and organic synthesis, specifically relating to a stable 3-aminocyclobutane-1,1-dicarboxylic acid diethyl oxalate and its application in the preparation of 3-(cholate-valerate)cyclobutane-1,1-dicarboxylic acid. This invention effectively solves the technical bottlenecks of poor stability, difficulty in purification, and storage by converting unstable free 3-aminocyclobutane-1,1-dicarboxylic acid diethyl oxalate into a specific organic acid salt form. More importantly, this invention discovers that this organic acid salt can be directly used as a reaction intermediate, and can be used efficiently in subsequent amide condensation reactions with cholic acid or its active derivatives without the separation of free amines. This method significantly improves the purity and yield of the key intermediate and its downstream product 3-(cholate-valerate)cyclobutane-1,1-dicarboxylic acid. The process is stable and simple to operate, providing a stable, controllable, and economical preparation route for the industrial production of 3-(cholate-valerate)cyclobutane-1,1-dicarboxylic acid, a key active pharmaceutical ingredient in the liver cancer prodrug LLC-202.

This invention belongs to the field of antibacterial materials, specifically relating to a composite antibacterial material modified with vancomycin through magnetically responsive bond grafting. The material comprises a magnetocaloric material, a polymer coated with the magnetocaloric material having free amino and / or hydroxyl groups, and vancomycin grafted onto the polymer via magnetron sputtering linkage residues. The magnetocaloric material is a functional material capable of generating magnetocaloricity under an alternating magnetic field. The magnetron sputtering linkage residues contain magnetically responsive bonds that can break under an alternating magnetic field, and these magnetically responsive bonds are azo bonds. This invention also provides a method for preparing the material and its applications. This invention has found that the composite material, based on the combined control of the components and modification methods, can unexpectedly achieve synergistic effects, thus effectively improving antibacterial activity and antibacterial spectrum. Furthermore, it can achieve magnetron sputtering of vancomycin, thereby achieving a magnetron sputtering attenuation effect.

The present application relates to a kind of animal-source-free complex protein hydrolysate and its preparation method, the weight percentage of the peptide segment with molecular weight less than or equal to 1000Da in the animal-source-free complex protein hydrolysate is greater than or equal to 90% in the total peptide segment of the animal-source-free complex protein hydrolysate, the weight percentage of protein is greater than or equal to 65%, the animal-source-free complex protein hydrolysate is prepared by the method comprising the raw material containing plant protein and yeast is enzymolysis.The animal-source-free complex protein hydrolysate of the present application has the natural attribute of animal-source-free, is rich in small molecule polypeptide and contains a certain amount of free amino acid, nucleotide and other components, while having the characteristics of not strictly dependent on exogenous protease, processing flexibility, product plasticity, wide application field, etc., can be used in cell culture, microbial fermentation, animal nutrition, plant nutrition and other fields.

The present application relates to the technical fields of biological medicine manufacturing, pharmaceutical excipients and preparation manufacturing, and discloses a preparation method of immune-enhanced bovine colostrum powder biological activity protection microcapsule, which is constructed with multi-layer microcapsule structure by multi-component active core, Fc segment protection stable layer, complex coagulation physical barrier layer, double response enteric layer and optional medication time period self-adaptive delay layer, effectively protects heat-sensitive active ingredients such as IgG, lactoferrin and lysozyme in the processes of low-temperature powdering, freeze-drying core making, electrostatic complex coagulation and fluidized bed coating, inhibits Maillard glycosylation reaction in storage period by non-reducing sugar vitrification matrix, free amino acid competitive space agent and activated molecular sieve, maintains FcRn receptor binding activity, reduces shelf life hidden failure, realizes low release in stomach, concentrated release under intestinal conditions, improves stability, targeting, bioavailability and application consistency under different medication time periods.

The application provides a bacillus natto and application thereof in preparation of functional feed for Chinese mitten crab breeding, and belongs to the technical field of aquaculture. Bacillus natto HQ-1 has been preserved in China Center for Type Culture Collection on August 11, 2025, and the preservation number is CCTCC No. M 20251811. Research shows that the bacillus natto has a water product pathogenic bacteria inhibition effect, a toxin degradation capacity and a water body environment purification function which are significantly improved compared with the same strain. The functional feed prepared by fermentation of the strain contains bioactive ingredients such as probiotic live bacteria, free amino acids, antibacterial lipopeptides and polyglutamic acid, can improve the nutritional form of the feed, improve the body weight, disease resistance and feeding rate of the Chinese mitten crab, and help to reduce the content of harmful substances in the aquaculture water body and improve the water body environmental quality. The application provides a new technical approach for green, antibiotic-free and sustainable breeding of the Chinese mitten crab.

This invention discloses a stability-improved complete thyroid hormone antigen, its preparation, and its application. The free amino group of the thyroid hormone or its deiodinated derivative moiety of this complete antigen is protected by a tert-butoxycarbonyl (Boc) group, and its carboxyl group is coupled to a carrier protein via an amide bond. The preparation method includes: protecting the amino group of a thyroid hormone molecule (such as T4, T3, etc.) with Boc2O, followed by activating its carboxyl group using the EDC / NHS method and coupling it to a carrier protein. This invention effectively overcomes the chemical instability problems of traditional complete antigens, such as easy photo-oxidative deiodination, easy racemization, and easy deamination reactions. In vitro detection reagents coated with the complete antigen of this invention exhibit longer shelf life, lower batch-to-batch variability, and extremely stable detection performance.

This invention discloses a liquid fertilizer containing small-molecule organic matter and its preparation method, comprising the following components by weight: 30-50 parts humic acid, 15-25 parts fulvic acid, 20-40 parts free amino acids, 20-40 parts urea, 15-30 parts ammonium dihydrogen phosphate, 15-35 parts potassium sulfate, 5-15 parts EDTA-chelated trace elements, 2-8 parts composite stabilizer, and 150-250 parts water; the mass ratio of fulvic acid to humic acid is 1:1.5-2.5; the free amino acids... The mass ratio of amino acids to fulvic acid is 1:0.5-1.2; the proportion of organic matter with a molecular weight less than 1000 Da in the liquid fertilizer is ≥65%; macromolecular organic matter is efficiently converted into small molecular form through microbial enzymatic decoupling process; humic acid, fulvic acid and free amino acids are used to give full play to the synergistic effect of multiple organic components; through multi-stage synergistic chelation process, the chelation rate of trace elements can reach more than 90%; the product does not separate after being stored at room temperature for more than 12 months.

Enzymatic Preparation and Immunological Application of Small Molecule Turtle Peptides. The method for preparing turtle peptides includes: providing turtle protein raw materials; performing a primary enzymatic hydrolysis of the turtle protein raw materials using an alkaline protease; performing a secondary enzymatic hydrolysis of the primary hydrolysis product using a complex enzyme containing flavor protease and trypsin; and inactivating, separating, and purifying the secondary hydrolysis product to obtain turtle peptides. This invention efficiently degrades large molecule turtle protein into small peptides and oligopeptides with an average molecular weight of less than 1000 Da, especially 180-800 Da, through stepwise synergistic enzymatic hydrolysis. These small peptides can be directly and completely absorbed through the intestinal peptide transport system, with an absorption rate and efficiency far exceeding that of free amino acids and whole proteins, significantly improving bioavailability. The small molecule turtle peptides prepared by this invention can significantly enhance the phagocytic capacity of macrophages and promote the proliferation and differentiation of lymphocytes, thereby enhancing the body's immune function at both the innate and adaptive immune levels.

The application discloses a preparation method of bovine placenta peptide and application thereof, and belongs to the technical field of biological products. The bovine placenta peptide is prepared from the placenta of a Galla Sahiwal cow, and the specific preparation steps comprise raw material pretreatment, complex enzymolysis, enzyme inactivation and separation, desalting and decoloration, concentration and drying. The complex enzymolysis adopts trypsin and neutral protease, and the adding ratio of the trypsin to the neutral protease is 3:2. More than 90% of the peptide segments in the obtained bovine placenta peptide have molecular weights distributed in the range of 500-1500 Da, the protein content is greater than 90%, and the free amino acid content is less than 3%. The bovine placenta peptide has a narrow peptide molecular weight distribution range, strong antioxidant and immunoregulatory activities, and health care functions of preventing and assisting in treating lung nodules and premature ovarian failure.

This invention discloses a bio-organic fertilizer, its preparation method, and its application, belonging to the field of bio-organic fertilizer technology. The invention separates dead silkworms and / or silkworm moths into two parts. The first part undergoes preservation, sterilization, and modification treatments, causing partial hydrolysis of proteins into small peptides and free amino acids, and degradation of chitin into chitosan oligosaccharides, yielding modified silkworm body material. The second part undergoes carbonization treatment to obtain silkworm biochar. The modified silkworm body material, silkworm biochar, and silkworm excrement are compounded in a weight ratio of (10-40):(10-40):(50-80), inoculated with a compound microbial agent, and subjected to aerobic fermentation to obtain the bio-organic fertilizer. This invention achieves the synergistic utilization of dead silkworms, silkworm moths, and silkworm excrement for the first time, overcoming the technical prejudice that dead silkworms "can only be discarded." The product has a total nitrogen content of 5%-7%, possessing both a readily available nitrogen source and the plant immune-inducing function of chitosan oligosaccharides, realizing the complete closed-loop utilization of silkworm breeding waste. This invention can be widely applied to the planting of mulberry trees, rice, rapeseed, corn, wheat, sugarcane, beans, and other cash crops.

This invention discloses a method for fermenting fresh almond flowers with *Lactobacillus plantarum*, the fermentation products, derivatives, and their applications. The method uses fresh almond flowers as raw material. After pretreatment, pulping, substrate preparation, and sterilization, *Lactobacillus plantarum* is inoculated for anaerobic or microaerobic fermentation. After fermentation, enzymes are inactivated and separated to obtain the fermentation expression product. This fermentation expression product is rich in free amino acids, small peptides, aromatic compounds, organic acid derivatives, and polyphenol derivatives, exhibiting a mild and natural aroma and excellent antioxidant and soothing activities. This invention also provides derivatives obtained from further processing of this fermentation expression product, and their applications in cosmetics, skincare products, natural fragrances, and functional foods. This invention features a mild process, is solvent-free, and highly safe, achieving high-value utilization of fresh almond flowers.