A green making technology of animal tissue paraffin section disclosed in the invention aims at providing a technique of making a paraffin section of animal tissue applied to the agricultural university medicine and other majors, characterized by that the type of chemical reagents that are used is limited, the chemical reagents have no toxicity, and dehydrate the tissue thoroughly, accelerate making sections but not lead to excessive contraction and increasement of hardness of the tissue, and substitute dimethyl benzene used in the traditional making technology of the paraffin section which isharmful to human body, so as to eliminate the occupational diseases and environmental pollution caused by dimethyl benzene. The making technology comprises the following steps: carrying out fixation with a fixative solution (100ml of the fixative solution comprises 91 ml 85% alcohol, 4 ml methanol and 5 ml glacial acetic acid ), dehydrating the tissue with graded ethanol, then putting the fixative and dehydrated tissue in a mixed transparent reagent (comprising 14wt% of analytically pour n-butanol, 29wt% of acetone and 57wt% of absolute alcohol ) for standing for 3 hours, then carrying out normal waxing and embedding, using analytically pour turpentine (60 DEG C) for section staining, carrying out dewaxing twice, carrying out hydration under normal temperature, using haematoxylin for staining nuclei, decoloration and bluing, and using eosin for staining, dehydrating until get absolute alcohol, then carrying out air drying, and directly using the gum diluted by turpentine to sealing the section.