32 results about "High throughput proteomics" patented technology

Methods to obtain expression systems and proteins in a high-throughput protocol by utilizing mixtures of cells cultured from those transformed with a desired nucleotide sequence permit rapid production of protein for use in arrays to assess activity. In one embodiment, the proteins (or peptides) in the array are assessed for their immunological activity with regard to an infectious agent.

This invention related to methods useful in the labeling of multiple polypeptide samples and subsequent analysis of these samples by mass spectrometry, particularly in the high throughput proteomic setting.

The invention relates to the technique of protein negative staining detection, in particular to an application of an eosin and a derivative of the eosin in protein negative staining detection. A method using the eosin to perform protein negative staining detection is further provided, which comprises the steps of fixing a gel of a protein sample after electrophoresis in a stationary liquid, washing with deionized water, performing equilibrium in a methanol solution, staining and developing. The application of the eosin and the derivative of the eosin in protein detection have the advantages of being high in sensitivity, simple and quick in operation, good in reproducibility, linear relation and mass spectrum compatibility, safe in usage, low in cost, applicable to the research of high throughput proteomics and the like.

The invention relates to the field of specific fluorescence detection technology of glycoprotein in the research orientation and in particular relates to synthesis of 1-pyrenyl-carbohydrazide (UGF202) and application of derivatives of 1-pyrenyl-carbohydrazide in specific detection of glycoprotein. The invention provides a method for performing specific fluorescence detection on glycoprotein by virtue of 1-pyrenyl-carbohydrazide. The method comprises the following four steps: fixing gel containing a protein sample subjected to electrophoretic separation in a fixing solution; oxidizing with a periodic acid solution; washing with vitamin C aqueous solution; and staining. The method has extremely high sensitivity, can be used for detecting glycoprotein lowering to 0.5ng, has the advantages of high specificity, simple and convenient operation steps, time conservation, high reproducibility, good linear relation, high mass spectrum compatibility, safety in use and low cost and can be well applied to research of high-flux proteomics.

The invention discloses an unlabeled proteomics method for trace cell online separation tandem mass spectrometry detection, and relates to the field of single cell proteomics detection. The method realizes cell separation, sample preparation and tandem mass spectrometry detection by a microfluidic system. According to the invention, accurate counting of cells and pretreatment of trace biological samples are realized by establishing the microfluidic system, so that accurate quantification of protein information of a single cell is realized; and high-throughput proteomics detection of the CTCs is further carried out, and the drug resistance reaction of individuals to chemotherapy is monitored by utilizing proteomics information of the CTCs, so that the method has great significance in further guiding individualized medication.

Methods to obtain expression systems and proteins in a high-throughput protocol by utilizing mixtures of cells cultured from those transformed with a desired nucleotide sequence permit rapid production of protein for use in arrays to assess activity. In one embodiment, the proteins (or peptides) in the array are assessed for their immunological activity with regard to an infectious agent.

The invention relates to a detection technology adopting a specific fluorescence pre-staining detection method of glycoprotein, and in particular relates to synthesis of 1-pyrenyl-carbohydrazide (UGF202) and an application of a derivative of UGF202 in specific fluorescence pre-staining detection of glycoprotein. The invention also provides a method for performing specific fluorescence pre-staining detection of the glycoprotein by applying UGF202. The method comprises the following steps: adding a periodic acid solution into a protein sample to perform oxidation; adding an ascorbic acid solution to neutralize the excess periodic acid solution; adding a dye for reaction; adding a sampling buffer solution; performing electrophoresis; and observing. The pre-staining detection method has the advantages of high sensitivity, good selectivity, simple and quick operation, good reproducibility, good linear relation, good mass spectrometry compatibility, safety in use, low cost and the like. Moreover, compared with a glycoprotein staining method after gel electrophoresis, the pre-staining detection method can realize observation after electrophoresis, so that a staining step after the gel electrophoresis is saved, and the pre-staining detection method can be well applied to researches of high-flux proteomics.

The invention relates to glycoprotein specific fluorescence detection technology, and particularly relates to application of 4 H-[1]-benzo pyran [4, 3-b] thiophene-2-formic acid hydrazide and a derivative thereof in glycoprotein specific fluorescence detection. The invention also provides a method for the application of the 4 H-[1]-benzo pyran [4, 3-b] thiophene-2-formic acid hydrazide for glycoprotein specific fluorescence detection, and the method comprises the steps of: fixation of after-electrophoresis gel containing a protein sample in a fixing solution; oxidation with periodic acid solution; washing with acetic acid aqueous solution; dyeing; and elution. The invention has the advantages of high sensitivity, good selectivity, fast and simple operation, good reproducibility, good linear relationship, good mass spectrum compatibility, safe use, low cost, and the like, and can be better suitable for the study of high-throughput proteomics.

The invention relates to a specific fluorescence detection technology of glycoprotein and particularly relates to an application of dansylhydrazine and derivatives thereof in specific detection of the glycoprotein. The invention also provides a method of performing specific fluorescence detection of the glycoprotein with the dansylhydrazine, wherein the method includes the steps of fixing a gel containing a protein sample after electrophoresis in a fixing liquid, oxidizing the gel with a periodic acid solution, washing the gel with acetic acid water solution; dyeing the gel, and eluting the gel. The technology is high in sensitivity and selectivity, is simple and rapid in operation, is good in repeatability, linear relationship and mass spectrum compatibility, is safe in use and is low in cost. The technology can be used for researching of high-flux proteomics well.

The invention relates to a protein negative staining detection technology, and particularly relates to 4', 5'-dibromo fluorescein and application of its derivative in protein detection. The invention further provides a method of performing protein negative staining detection by applying the 4', 5'-dibromo fluorescein. The method includes steps of directly dyeing after finishing the protein gel electrophoresis, and then developing. The 4', 5'-dibromo fluorescein has advantages of high sensitivity, simple operation, good reproducibility, good mass spectrum compatibility, low cost, and others, and can be well applicable to the study of high-flux proteomics.

The invention relates to a protein negative staining detection technology, in particular to 2',7'-dichlorofluorescein and application of a derivative thereof in protein negative staining detection. The invention further provides a method for performing protein negative staining detection by applying the 2',7'-dichlorofluorescein. The method comprises the steps that direct dyeing is performed after protein gel electrophoresis is finished; then, developing is performed. The 2',7'-dichlorofluorescein has the advantages of being high in sensitivity, easy and rapid to operate, good in reproducibility, good in linear relation, good in mass spectrum compatibility, safe to use, low in cost and the like, an can be well applicable to research on high-throughput proteomics.

The present invention relates to a specific fluorescent pre-dyeing detection method detection technology of glycoproteins, particularly to applications of 4H-[1]-benzopyran[4,3-b]thiophene-2-carboxylic acid hydrazide and a derivative thereof in a specific fluorescent pre-dyeing detection method of glycoproteins. The invention further provides a method for using 4H-[1]-benzopyran[4,3-b]thiophene-2-carboxylic acid hydrazide to carry out specific fluorescent pre-dyeing detection of glycoproteins, wherein the method comprises: adding a periodic acid solution to a protein sample to oxidize, adding an ascorbic acid solution to neutralize the excess periodic acid solution, adding a dye to carry out a reaction, adding a loading buffer, carrying out electrophoresis, and observing. According to the present invention, the pre-dyeing has advantages of high sensitivity, good selectivity, simple and rapid operation, good reproducibility, good linearity, good MS compatibility, use safety, low cost, and the like; and compared with the after-gel electrophoresis glycoprotein dyeing method, the pre-dyeing detection method has the following characteristics that the observation can be performed after completing the electrophoresis so as to eliminate the dyeing step after the gel electrophoresis, and the method can be well suitable for the high-throughput proteomics research.

The invention discloses application of a medium-chain fatty acid monoglyceride composition in preparation of a product for promoting bone growth, and relates to the technical field of medicines and health care products. The invention researches the influence of medium-chain fatty acid on bone growth and bone metabolism by utilizing a high-throughput proteomics technology, and finds that the composition of lauric acid monoglyceride, capric acid monoglyceride and caprylic acid monoglyceride can promote bone growth, increase bone density and improve bone metabolism. A safe and healthy intervention scheme is provided for patients with osteoporosis, chronic obstructive pulmonary diseases and metabolic disorders related to reduction of bone mineral density, and the method has excellent practicability.

The present invention relates to a specific fluorescent pre-dyeing detection method, specifically to applications of dansylhydrazine and a derivative thereof in a specific fluorescent pre-dyeing detection method of glycoproteins. The invention further provides a method for using dansylhydrazine to carry out specific fluorescent pre-dyeing detection of glycoproteins, wherein the method comprises: adding a periodic acid solution to a protein sample to oxidize, adding an ascorbic acid solution to neutralize the excess periodic acid solution, adding a dye to carry out a reaction, adding a loading buffer, carrying out electrophoresis, and observing. According to the present invention, the pre-dyeing has advantages of high sensitivity, good selectivity, simple and rapid operation, good reproducibility, good linearity, good MS compatibility, use safety, low cost, and the like; and compared with the after-gel electrophoresis glycoprotein dyeing method, the pre-dyeing detection method has the following characteristics that the observation can be performed after completing the electrophoresis so as to eliminate the dyeing step after the gel electrophoresis, and the method can be well suitable for the high-throughput proteomics research.

Compositions and methods for sample preparation and mass spectrometric analysis of peptide samples obtained from biological samples are provided. The compositions and methods include a tandem column system in which a trap column is in fluid contact with an analytical column such as, for example, a HPLC column. As analytes are eluted from the analytical column, they can be passed to a detector (e.g., a mass spectrometer) for peptide analysis.