32 results about "High throughput proteomics" patented technology

A combinatorial method that uses statistics and DNA sequence analysis rapidly assesses the functional and structural importance of individual protein side chains to binding interactions. This general method, termed “shotgun scanning”, enables the rapid mapping of functional protein and peptide epitopes and is suitable for high throughput proteomics.

This invention related to methods useful in the labeling of multiple polypeptide samples and subsequent analysis of these samples by mass spectrometry, particularly in the high throughput proteomic setting.

The invention discloses a stable isotope-based high-throughput proteomic quantitative reagent, which can also be called an isobaric Labeling (IBT) reagent, and the structure of the stable isotope-based high-throughput proteomic quantitative reagent comprises the formula of reporter group-balance group-activation group. Such reagent can be accomplished by different types of chemical structures. Theinvention discloses eight groups of different chemical structures, and each group of structures can comprise up to 10 label reagents (represented by IBT-10PLEX) with completely identical chemical structures. However, 13C or 15N isotopes (which can be represented by T-114, T-115N, T-115C, T-116N, T-116C, T-117N, T-118N, T-117C, T-118C and T-119 respectively) are contained at different positions ineach label reagent. The invention discloses a preparation method of three groups of isobaric tag reagents, and further discloses a quantitative analysis method of polypeptide by adopting the IBT-10PLEX reagent. The IBT-10PLEX disclosed by the invention adopts a novel chemical structure, the preparation difficulty is reduced, and the yield is improved, so that the product cost can be reduced, andthe application range of an isobaric labeling method is expanded.

The present application provides compositions and methods for sample preparation and mass spectrometry analysis of peptide samples obtained from biological samples. The compositions and methods include in-line column systems in which a trapping column is in fluid contact with an analytical column such as, for example, an HPLC column. As the analytes are eluted from the analytical column, they can be passed to a detector (eg, a mass spectrometer) for peptide analysis.

The invention relates to the field of specific fluorescence detection technology of glycoprotein in the research orientation and in particular relates to synthesis of 1-pyrenyl-carbohydrazide (UGF202) and application of derivatives of 1-pyrenyl-carbohydrazide in specific detection of glycoprotein. The invention provides a method for performing specific fluorescence detection on glycoprotein by virtue of 1-pyrenyl-carbohydrazide. The method comprises the following four steps: fixing gel containing a protein sample subjected to electrophoretic separation in a fixing solution; oxidizing with a periodic acid solution; washing with vitamin C aqueous solution; and staining. The method has extremely high sensitivity, can be used for detecting glycoprotein lowering to 0.5ng, has the advantages of high specificity, simple and convenient operation steps, time conservation, high reproducibility, good linear relation, high mass spectrum compatibility, safety in use and low cost and can be well applied to research of high-flux proteomics.

A method of analyzing a sample comprising multiple protein species is provided. The proteins are separated by species such that the multiple protein species emerge in a sequential order and are then digested in the sequential order in which they emerge from the separation process. The digested proteins are introduced into a mass spectrometer in the same sequential order so that, within a given time window, the digested proteins introduced into the mass spectrometer are covariant.

Methods to obtain expression systems and proteins in a high-throughput protocol by utilizing mixtures of cells cultured from those transformed with a desired nucleotide sequence permit rapide production of protein for use in arrays to assess activity. In one embodiment, the proteins (or peptides) in the array are assessed for their immunological activity with regard to an infectious agent.

The invention relates to glycoprotein specific fluorescence detection technology, and particularly relates to application of 4 H-[1]-benzo pyran [4, 3-b] thiophene-2-formic acid hydrazide and a derivative thereof in glycoprotein specific fluorescence detection. The invention also provides a method for the application of the 4 H-[1]-benzo pyran [4, 3-b] thiophene-2-formic acid hydrazide for glycoprotein specific fluorescence detection, and the method comprises the steps of: fixation of after-electrophoresis gel containing a protein sample in a fixing solution; oxidation with periodic acid solution; washing with acetic acid aqueous solution; dyeing; and elution. The invention has the advantages of high sensitivity, good selectivity, fast and simple operation, good reproducibility, good linear relationship, good mass spectrum compatibility, safe use, low cost, and the like, and can be better suitable for the study of high-throughput proteomics.

The present invention relates to a specific fluorescent pre-dyeing detection method detection technology of glycoproteins, particularly to applications of 4H-[1]-benzopyran[4,3-b]thiophene-2-carboxylic acid hydrazide and a derivative thereof in a specific fluorescent pre-dyeing detection method of glycoproteins. The invention further provides a method for using 4H-[1]-benzopyran[4,3-b]thiophene-2-carboxylic acid hydrazide to carry out specific fluorescent pre-dyeing detection of glycoproteins, wherein the method comprises: adding a periodic acid solution to a protein sample to oxidize, adding an ascorbic acid solution to neutralize the excess periodic acid solution, adding a dye to carry out a reaction, adding a loading buffer, carrying out electrophoresis, and observing. According to the present invention, the pre-dyeing has advantages of high sensitivity, good selectivity, simple and rapid operation, good reproducibility, good linearity, good MS compatibility, use safety, low cost, and the like; and compared with the after-gel electrophoresis glycoprotein dyeing method, the pre-dyeing detection method has the following characteristics that the observation can be performed after completing the electrophoresis so as to eliminate the dyeing step after the gel electrophoresis, and the method can be well suitable for the high-throughput proteomics research.

The invention relates to protein negative staining detection technology, in particular to the application of eosin and its derivatives in protein negative staining detection. The invention also provides a method for protein negative staining detection using eosin, comprising the steps of: fixing the gel after protein sample electrophoresis in a fixative solution, washing with deionized water; equilibrating with methanol solution, staining; and developing. The invention has the advantages of high sensitivity, simple and rapid operation, good reproducibility, good linear relationship, good mass spectrometry compatibility, safe use, low cost, etc., and can be better applied to the research of high-throughput proteomics.

The invention relates to a protein negative staining detection technology, in particular to 2',7'-dichlorofluorescein and application of a derivative thereof in protein negative staining detection. The invention further provides a method for performing protein negative staining detection by applying the 2',7'-dichlorofluorescein. The method comprises the steps that direct dyeing is performed after protein gel electrophoresis is finished; then, developing is performed. The 2',7'-dichlorofluorescein has the advantages of being high in sensitivity, easy and rapid to operate, good in reproducibility, good in linear relation, good in mass spectrum compatibility, safe to use, low in cost and the like, an can be well applicable to research on high-throughput proteomics.