142 results about "Endosperm" patented technology

The invention discloses a corn germplasm resource viability cross-germplasm discrimination method, system, equipment and medium, and relates to the technical field of corn viability discrimination, in the method, hyperspectral data of an embryo area and hyperspectral data of an endosperm area are obtained; obtaining hyperspectral data of each sample corn seed; each piece of hyperspectral data is preprocessed; feature selection is carried out on each piece of preprocessed hyperspectral data, and a constructed double-branch spectrum Transform corn germplasm cross-germplasm viability detection model is trained in combination with viability label data of each sample corn seed; the trained double-branch spectrum Transform corn germplasm cross-germplasm viability detection model is utilized to discriminate the cross-germplasm corn to be discriminated, the viability prediction result of the cross-germplasm corn to be discriminated is obtained, and the accuracy and reliability of cross-germplasm corn germplasm resource viability discrimination can be remarkably improved.

The present invention relates to the field of plant genetic engineering technology, specifically providing a rice endosperm tissue-specific promoter, pPROLM26, and its applications. The present invention isolates a tissue-specific promoter with the nucleotide sequence shown in SEQ ID NO. 1 from a rice alcohol-soluble protein gene. This tissue-specific promoter is truncated four times and then molecularly identified with a RUBY visualization marker to obtain truncated promoter fragments shown in SEQ ID NOs. 2 to 5, each with endosperm-specific promoter function. The expression product has been measured to contain up to 2.5% betaine of the seed dry weight, demonstrating its high application value in the field of plant synthetic biology. The present invention further discloses applications of the promoter or truncated promoter fragments in improving crop seed quality, improving crop traits, cultivating new transgenic plant varieties using key elements of the promoter, or expressing high-value-added proteins using seed bioreactors.

This invention discloses a rice starch synthesis-related gene, OsFLO17, its encoded protein, and its applications. Through phenotypic analysis of the rice endosperm mealy mutant flo17 and preliminary localization of the target gene, the starch synthesis-related protein OsFLO17 was ultimately cloned. This protein consists of the amino acid sequence shown in SEQ ID NO.3. The starch granule development-related protein of this invention affects the rice endosperm starch synthesis process. Introducing the encoding gene of this protein into plants with abnormal starch granules can yield transgenic plants with normal starch filling. Therefore, the protein and its encoded gene of this invention can be applied to plant genetic improvement.

The application provides a rice heart-white gene WCG1 and application thereof, and particularly relates to positioning, cloning, function verification and application of a WCG1 gene for controlling a rice kernel heart-white phenotype. A C / A natural variation exists at a ninth exon 986 site of the WCG1 gene, the variation site makes the rice kernel present a heart-white phenotype, that is, a kernel middle heart-white and edge transparent phenotype, and the phenotype is an excellent phenotype of "sake brewing good suitable rice" specially selected in sake brewing.

The present application relates to rice breeding technical field, and relates to a low glycemic index purple rice breeding method. The method comprises the following steps: taking RG4 as the father and taking Zheda Zi 1S as the mother to obtain F1 generation seeds by hybridization; planting the F1 generation seeds, taking the F1 generation as the father and taking Zheda Zi 1S as the mother to obtain BC1F1 generation seeds by backcrossing; planting the backcrossed BC1F1 generation seeds, and obtaining BC1F2 generation seeds by selfing; planting the BC1F2 generation seeds, harvesting normal seed setting fertile excellent single plants, shelling and selecting purple rice, processing into whole white non-waxy rice with endosperm, and then detecting and selecting high resistance starch content strains; planting the BC1F3 generation seeds, selecting and reserving the strains with stable purple rice traits, and determining and selecting strains with whole white endosperm and high resistance starch content; planting the BC1F4 generation seeds, determining and selecting low GI strains; planting the BC1F5 generation seeds, selecting and reserving strains with high alkali solubility value and easy cooking by taking good cooking as the screening index, and breeding low GI purple rice. The rice obtained by the method particularly meets the individual nutritional needs of diabetic patients.

The invention discloses a breeding method and application of fragrant soft rice two-line hybrid rice with an off-white anti-counterfeiting mark, and belongs to the technical field of rice breeding. According to the method, soft rice genes with recessive grey white endosperm markers, genic male sterility character genes and the like are subjected to synchronous phenotype aggregation directional selection for the first time, the problem that the genic male sterility character and excellent taste quality character are difficult to synchronously select in the earliest segregation generation is solved for the first time, and in addition, the technical link of taste quality detection and identification can be saved; the selection efficiency is improved; meanwhile, the problem that the taste quality and control genes thereof need to be detected and identified for multiple times during mating and parent selection of high-quality hybrid rice is solved for the first time; besides, the soft rice character gene with the recessive grey white endosperm marker is synchronously introduced into a two-line hybrid rice parent, through main target character aggregation response identification, the fragrant soft rice two-line hybrid rice with the grey white marker and the aromatic flavor is successfully created for the first time, the yield is increased, and infringement and loss of independent brand fragrant rice products can be prevented.

The utility model provides a corn degerming post-treatment winnowing device. The corn degerming post-treatment winnowing device comprises an air bellow, an air-closed feeder and an air-closed discharger, the closed air feeder is arranged on the top wall of one end of the air bellow and is used for continuously feeding a mixed material of corn endosperm and corn bud skin into the air bellow; the closed-air discharger is arranged on the bottom wall of the other end of the air bellow; a blanking box is arranged on the bottom wall of the air bellow and right below the closed air feeder; an air inlet channel is arranged on the side wall of one end, close to the blanking box, of the air bellow and is used for blowing air into the air bellow; an air outlet pipe is arranged on the top wall of the air bellow and right above the closed air discharger; wherein a flow guide assembly is arranged in the air bellow, and the flow guide assembly is used for guiding air blown into the air bellow to the air outlet pipe and further used for guiding corn endosperm and corn bud husks which are separated according to the gravity difference under the action of wind power to the blanking box and the closed air discharger respectively. According to the corn degerming post-treatment winnowing device, the endosperm winnowing extraction efficiency and purity after corn degerming can be improved.

The invention relates to low-damage endosperm rice and a preparation process thereof. Specifically, the invention provides a brown rice milling method, the method comprises the steps of unhusked rice opening and fine milling, and the method comprises the step of controlling the protein retention rate, the damaged starch content and the protein-to-ash ratio of milled brown rice to be in the following ranges: the protein retention rate is 88.0% or above, the damaged starch content is 0.480% or below, and the ash-to-protein ratio is 0.055 or above.

The invention belongs to the field of plant engineering technology and crop germplasm resource innovation, and particularly relates to a rice BEIIb gene mutant, application of the rice BEIIb gene mutant in improving rice resistant starch and an improving method. A mutant material with high resistant starch content is created by knocking out the rice BEIIb gene, and compared with a wild type, mutant seeds show an obvious chalky phenotype, composite starch particles are abnormal in a spherical or ellipsoidal shape, the amylose content is remarkably increased, the protein content is remarkably reduced, the resistant starch content is remarkably increased, and the resistant starch content is remarkably reduced. The hardness of cooked rice is remarkably increased, and the elasticity is remarkably reduced. Through transcriptomics analysis, the protein mainly participates in biological pathways such as carbohydrate metabolism, chloroplast photosynthesis, calmodulin combination and secondary metabolite synthesis, and the expression level of endosperm starch synthesis related genes is obviously changed. The method can provide theoretical basis and method reference for breeding of high-quality rice varieties with high resistant starch.

The invention discloses an efficient corn germ extracting machine which comprises a bottom plate, two mounting rods are fixedly mounted at the top of the bottom plate, a same fixing plate is fixedly mounted at the tops of the two mounting rods, a hydraulic cylinder is fixedly mounted at the bottom of the fixing plate, and a follower plate is arranged below the hydraulic cylinder; and a filter basin is arranged below the follower plate. The hydraulic cylinder drives the driving plate and the follow-up plate to synchronously move downwards, so that the follow-up plate is tightly attached to the top end of the filter basin to achieve sealing of the top end, dust overflowing and material splashing are effectively avoided, then the hydraulic cylinder continues to push the driving plate to move downwards to start the servo motor, the rolling block rotates at a high speed while swinging and rolling, and the dual treatment effect is achieved; the separation efficiency and purity of corn germs and endosperm are greatly improved, in the treatment process, dust and chippings in the filter basin can be sucked and collected, dust harm is reduced, finally, a hydraulic cylinder reversely drives a driving plate to move upwards, back flushing cleaning is conducted on the inner wall of the filter basin, and residual material accumulation is avoided.

The invention belongs to the field of gene engineering, and relates to a chalkiness-related protein OsGAPDH of rice endosperm as well as a coding gene and application of the chalkiness-related protein OsGAPDH. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO: 3 in a sequence table; and (b) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence of SEQ ID NO: 3, is related to plant starch synthesis and is derived from SEQ ID NO: 3. The protein provided by the invention affects normal development of rice endosperm. The coding gene of the protein is introduced into Ningjing No.7 wild type, so that a transgenic plant of which the chalkiness of endosperm is obviously reduced can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

[Problem] To provide a composition for regulating blood pressure for a healthy person. [Solution] A blood-pressure-regulating composition for a healthy person, said composition being characterized by containing a guar gum degradation product, and being characterized in that: 70 mass% or more of the guar gum degradation product has an average molecular weight within the average molecular weight range of 1.0 × 103 to 1.0 × 105; the composition has a viscosity of 10 mPa·s or less when a 2 mass% aqueous solution at 37°C is measured at 10 rpm using an E-type viscometer, or has a viscosity of 5 mPa·s or less when the aqueous solution is measured at 20 rpm, or has a viscosity of 3 mPa·s or less when the aqueous solution is measured at 30 rpm; the composition is obtained by hydrolyzing a galactomannan polysaccharide which contains guar-derived endosperm and in which the content ratio (galactose:mannose) of galactose and mannose is within the range of 1:1.3 to 1:2, by using a microorganism-derived β-mannanase to reduce the molecular weight of the polysaccharide; and the composition contains at least 70 mass% of dietary fiber in terms of the dietary fiber content stipulated in the enzyme-HPLC method.

The invention discloses a novel coronavirus variant RBD protein gene and application thereof. The method comprises the following steps: firstly, respectively constructing recombinant plant expression vectors pGt1-RBD and pGt1-RBDopt containing RBD and RBDopt genes, carrying out agrobacterium-mediated transformation to obtain a T0-generation transgenic rice plant, and carrying out T0-T3-generation screening and identification to obtain a transgenic rice plant capable of stably expressing RBD protein. The RBD protein is successfully expressed in rice endosperm and is subjected to N-glycosylation modification, and the content of the RBD protein in pGt1: RBDopt is obviously higher than that of pGt1: RBD. The pGt1:: RBDopt transgenic rice flour suspension is orally taken into an intragastric mouse, and experimental results show that the transgenic rice pGt1:: RBDopt can be used for developing oral vaccines of new crown vaccines and has a good application prospect.

Methods for producing a purified wheat embryo product are disclosed. In one embodiment, producing a purified wheat embryo product includes the steps of: accelerating a plurality of wheat berries toward an impact surface, impacting each of the plurality of wheat berries against the impact surface, dislodging at least some of the wheat embryos from the wheat berries in response to the impacting step such that the dislodged embryos are intact, and separating the dislodged wheat embryos from the bran and the endosperm to produce an intermediate purified wheat embryo product.

The invention discloses application of a rice OsBZR4 gene in cultivation of rice embryo-free seeds, relates to the technical field of gene engineering, and aims to provide application of the rice OsBZR4 gene in cultivation of rice embryo-free seeds, which has important practical significance and agricultural utilization value. A CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated 9) technology is utilized to perform targeted knockout on the rice gene OsBZR4, so as to obtain the rice gene OsBZR4 knockout According to the gene knockout mutant of the rice OsBZR4 gene under the background of different rice varieties, 60-90% of non-embryo seeds and 10-40% of small embryo and normal embryo seeds can be generated. On the premise that the yield and the quality character are not affected, the polished rice rate of the rice can be increased, and the storage resistance of the rice can be improved. Precious germplasm resources are provided for rice seed embryo and endosperm development research, and the method has very important scientific value for research of a rice seed embryo and endosperm development regulation mechanism.

The invention provides preboiled rice and a preparation method thereof, and relates to the field of rice processing. The preparation method comprises the following steps: removing impurities from rice and washing; the method comprises the following steps: putting rice into a container, adding chrysanthemum and warm water with the temperature of 43-48 DEG C, and soaking for 24-48 hours until the water content of the rice reaches 40-60%; air-drying rice surface moisture; steaming the rice for the first time, namely putting the rice treated in the step S3 into a steaming box, steaming for 15-20 minutes at 100-105 DEG C, and then cooling to 40-50 DEG C; performing secondary steaming, namely putting the rice treated in the step S4 into the steaming box, and steaming for 25-30 minutes at 110-115 DEG C; performing third-time steaming, namely putting the rice treated in the step S5 into the steaming box, and steaming for 10-15 minutes at the temperature of 90-95 DEG C; the rice treated in the step S6 is air-dried or air-dried until the water content is 20-25%; and S7, adding chrysanthemum powder into the rice treated in the step S7, then putting the rice into a sealed container, sealing for 24-48 hours, airing until the water content is less than 12%, and shelling to obtain the preboiled rice. Through three times of cooking at different temperatures, starch can be modified, and water-soluble nutrients in the resistant layer are gradually migrated to endosperm.

The invention belongs to the technical field of plant genetic engineering and molecular breeding, and particularly relates to a new function and application of a transcription factor gene OsMYB87 derived from rice, an OsMYB87 function deletion mutant created through a gene editing technology and application of the OsMYB87 function deletion mutant in increasing the rice grain length and grain weight. The OsMYB87 gene found in the invention shows a key differentiated characteristic: preliminary phenotypic observation shows that the phenomenon that chalkiness of endosperm is remarkably aggravated inevitably due to OsMYB73 knockout is not observed while the function deficiency of the OsMYB87 gene brings a remarkable yield increasing effect (grain length and grain weight increase). The OsMYB87 is strongly prompted to be possibly located on a different regulatory pathway, and as a breeding target, the OsMYB87 provides greater possibility and a better potential choice for maintaining the appearance quality of rice commodities while realizing yield increase, so that the OsMYB87 has higher application value.

The invention relates to a method for creating a rice material with low absorbable protein content and remarkably thickened endosperm cell walls and application of the rice material. Specifically, the invention provides a method for improving rice, and improved rice with unique characters is obtained by down-regulating the expression of glutelin genes and down-regulating the expression of globulin genes. In the improved rice, the content of absorbable protein is low, and endosperm cell walls are remarkably thickened. Besides, the improved rice is low in in-vitro digestion rate, has the potential postprandial blood sugar control effect and is suitable for preparing auxiliary food for treating chronic kidney disease patients, diabetic patients and the like.

The application discloses a kind of high altitude permafrost engineering disturbance area's vegetation recovery method, comprising the following steps: to meet sowing time period, turn over, supplement grass seed, sowing time period is: deep 0 cm place consecutive 10 days daily average ground temperature value is not less than first preset temperature value and deep 50~60 cm place consecutive 12 daily average ground temperature value is not less than second preset temperature value after the deadline 3~10 days;Cover multilayer covering net, until deep 0 cm place consecutive 8 days average ground temperature value is not less than third preset temperature value, remove part of covering net, only remain a layer of covering net;When grass seedling grows to 5~8 cm, remove last layer of covering net.The application is limited by the daily average ground temperature value of deep 0 cm in frozen soil disturbance area and deep 50~60 cm in frozen soil disturbance area, which helps to keep the endosperm cells inside active after seed sowing, increases the germination rate of grass seeds, and has good vegetation recovery effect.

The present application belongs to the technical field of coffee making, and particularly relates to a low-temperature extraction method of coffee liquid. The low-temperature extraction method of coffee liquid comprises the following steps: (1) peeling coffee fresh fruits to retain endocarp, endosperm and embryo, and obtaining peeled coffee beans; (2) adding water to the peeled coffee beans, sterilizing, then adding fermentation inoculum to obtain fermentation material, and carrying out anaerobic fermentation to obtain fermentation liquid; (3) after the fermentation is completed, sterilizing, drying, crushing to obtain coffee powder, mixing the coffee powder with water, then carrying out pressure treatment, filtering to obtain coffee extraction liquid and coffee residue; (4) drying the coffee residue, adding the residue into an extraction kettle to carry out supercritical carbon dioxide extraction, obtaining extraction, filtering to obtain extraction product; (5) mixing the coffee extraction liquid and the extraction product to obtain coffee liquid. The coffee liquid of the present application has suitable content of gallic acid, high antioxidant property, excellent taste and aroma.

The invention discloses application of a ZmEREB93 gene knockout biological material in cultivation of high-yield and high-quality corn, and relates to the technical field of biology. The ZmEREB93 gene knockout strain is constructed, homozygous seeds of the ZmEREB93 gene knockout strain are subjected to seed test analysis, and it is found that corn kernels of the knockout strain are remarkably superior to a wild type in the aspects of the grain length, the grain width, the hundred-grain weight and the single plant yield. In the early, middle and late periods of grain filling, the corn grains of the knockout strain are obviously superior to a wild type control in shape and endosperm structure. Further determination of starch content and protein content shows that the starch content of seeds of the knockout strain is significantly higher than that of a wild type control group, the alcohol-soluble protein content is also significantly increased, and the total protein content is significantly higher than that of a control group. Therefore, the ZmEREB93 gene has an important application value in cultivation of a high-yield and high-quality new corn variety.

The invention discloses an extraction and separation process of barley endosperm, and belongs to the field of grain extraction. The method specifically comprises the following steps that barley grains are cleaned and smashed in spring, then classified screening, hulling and sorting are conducted, pretreated endosperm powder is obtained through grinding, endosperm separation and drying are conducted, complete barley grains and broken or defective barley grains can be separated through classified screening, and reduction of loss of raw materials in the subsequent hulling and sorting process is facilitated. A separation accelerant is added in the endosperm separation process, calcium ions can react with hydroxyl contained in starch molecules to form a complex structure, the stability of starch particles is enhanced, the sugar alcohol compound can inhibit gelling of the starch molecules, especially amylose, through the competitive effect, the recovery rate of starch in barley endosperm is increased, and the yield of the barley endosperm is increased. And the centrifugal liquid obtained by separation in the separation process enhances the recovery of the by-product beta-glucan under the action of the selective adsorbent, reduces the influence of impurity molecules, and improves the recovery rate and purity of the beta-glucan.

The application discloses a method for identifying endochalazal interacting proteins in rice based on a TurboID proximity labeling technology, and belongs to the technical field of biology. The method comprises the following steps: firstly, forming a fusion protein by connecting a target protein with a TurboID biotin ligase, and constructing a transgenic rice plant for stably expressing the fusion protein; secondly, mixing a developing seed of the transgenic plant 7-9 days after flowering with a biotin solution with a concentration of 100-400 muM, performing vacuum treatment for 10-30 min, and then placing the seed for 8-36 h; and thirdly, taking a rice endochalazal sample treated by the biotin to perform protein extraction, enrichment and mass spectrometry identification on the biotin-labeled interacting proteins in the rice endochalazal. The application provides a method for performing proximity space labeling on a target protein in the rice endochalazal based on a TurboID system, and the method can be used for analyzing possible protein-protein interactions.

Methods for producing purified wheat germ products are disclosed. In one embodiment, producing a purified wheat germ product includes the steps of: accelerating a plurality of wheat kernels toward an impact surface; impacting each of the plurality of wheat kernels against the impact surface; in response to the impacting step, dislodging at least some wheat germ from the wheat kernel such that the dislodged germ is intact; and separating the dislodged wheat germ from bran and endosperm to produce an intermediate purified wheat germ product.

The present application relates to the technical field of microorganism or culture thereof processing, and particularly relates to a method for breeding pleurotus ostreatus mycelium by using highland barley as a substrate. The method is as follows: highland barley is crushed and sieved, then water is added to prepare highland barley slurry, and a complex enzyme system composed of amylase and cellulase is added to perform liquid enzymolysis to obtain an enzymolysis substrate; lactic acid bacteria strains are inoculated into the enzymolysis substrate to perform solid-state fermentation to obtain a fermentation substrate; the fermentation substrate is subjected to moist heat sterilization treatment to obtain a sterilized substrate; the sterilized substrate is inoculated with pleurotus ostreatus original seeds, and is placed in a culture environment for culture to obtain pleurotus ostreatus mycelium. The scheme breaks the physical barrier of the fiber network of the highland barley epidermis and the starch crystal structure of the endosperm by using the complex enzyme system and lactic acid bacteria fermentation, and the substrate is pre-degraded before the mycelium is inoculated, so that the mycelium can directly absorb free carbon sources, the germination time is shortened, the daily growth rate is improved, the residual sugar rate of the substrate is reduced, and the dry weight biomass of the mycelium is increased.

The invention belongs to the technical field of agricultural machinery, and discloses an ecological grassland vegetation restoration and reseeding machine which comprises a rack as an integral frame of the reseeding machine; the storage box is connected to the upper portion of the rack and used for containing seeds and feed; the supporting wheels are connected to the two sides of the rack, support the rack and assist the rack in moving along the ground; the annular air injection structure horizontally injects air along the inner wall of the pipeline, and a uniform horizontal air cushion layer is formed between the pipe wall and a seed falling path, so that the falling resistance of the seeds can be increased, and the falling speed of the seeds is reduced; the annular rotational flow air injection structure spirally and downwards injects air along the inner wall of the pipeline to form a rotating air curtain for wrapping seeds, the seeds are restrained near the central axis of the pipeline through centrifugal force of air flow, the transverse swing amplitude is reduced, the physical contact probability of the seeds and the pipe wall is reduced, and the problems of seed coat damage, endosperm loss and the like are effectively solved. Therefore, the seed activity is obviously protected.

The invention discloses a breeding method and application of fragrant soft rice three-line hybrid rice with an off-white anti-counterfeiting mark, and belongs to the technical field of rice breeding. According to the method, the problem that the taste quality and control genes thereof need to be detected and identified for multiple times during parent selection of high-quality hybrid rice is solved for the first time, consistency of the taste quality and the control genes thereof of the hybridized and matched parents is ensured, and the breeding cost is reduced; synchronous phenotype polymerization selection is carried out on an off-white endosperm marker gene representing excellent taste quality and a high combining ability, aromatic flavor and maintenance gene, so that the problem that taste quality selection is difficult to carry out on a high-quality hybridization maintenance single plant in a separation early generation is solved for the first time; in addition, soft rice marker genes with recessive grey-white endosperm are synchronously introduced into three-line hybrid rice parents, through main target character aggregation response identification, the fragrant soft rice three-line hybrid rice with grey-white markers in rice grains is successfully created for the first time, the yield is increased, and consumers can conveniently identify the authenticity of products and make brands.

The invention discloses a Saccharomyces mirabilis fermentation broth and a preparation method and application thereof, and the preparation method comprises the following steps: crushing or homogenizing coconut solid endosperm to prepare a fermentation medium; inoculating saccharomyces cerevisiae to the fermentation culture medium for fermentation; and after the fermentation is finished, carrying out separation treatment to obtain the saccharomyces cerevisiae fermentation liquor. Compared with the prior art, the saccharomyces mirabilis is selected as a fermentation strain, the coconut solid endosperm is taken as a main fermentation substrate, the metabolic characteristics of the strain are fully exerted under a mild fermentation condition, and the mannan in the coconut solid endosperm is efficiently converted into mannan oligosaccharide; the obtained saccharomyces cerevisiae fermentation liquor has excellent moisturizing and repairing effects and the like, and has a good application prospect in cosmetics.

The invention relates to a genetically modified rice plant or plant cell, which comprises a heterologous heavy metal ATP enzyme gene operably linked to an OsActin1 promoter, a heterologous ATP binding cassette (ABC) transporter gene operably linked to the OsActin1 promoter, and a heterologous plant chelating peptide synthase gene operably linked to the OsActin1 promoter, wherein the OsActin1 promoter has a lower activity in the seed endosperm of the modified rice plant compared to the activity in other nutritional tissues of the modified rice plant; wherein arsenic (As) and cadmium (Cd) in rice grains of the genetically modified rice plant are reduced as compared to a control rice plant in which the genetic modification is not performed. The invention also relates to a method for constructing such genetically modified rice plants or plant cells, and a kit for carrying out the method.

A method for obtaining highly sulfated nanoparticles of cellulose or sulfated nanoparticles of mannan, or a combination thereof, from endosperms, comprising acquiring a ground flour of an endosperm of a seed, nut, or pit, wherein the ground flour contains polysaccharides, and wherein the polysaccharides contain either cellulose or mannan, or both cellulose and mannan; pretreating the ground flour to condition the polysaccharides for hydrolysis and functionalization thereof; preparing a reagent for hydrolysis and functionalization of the polysaccharides, wherein the reagent comprises a solution of glycerol, water, and sulfuric acid at predetermined concentrations; adding the reagent to the pretreated polysaccharides to form a suspension, wherein adding the reagent to the suspension converts the cellulose or mannan or both into nanoparticles that are sulfated by the reagent; separating the sulfated nanoparticles from the suspension; washing the nanoparticles; and creating a stable colloidal suspension of the sulfated nanoparticles. The sulfated nanoparticles are used in pharmaceutical, cosmetic, wound healing, and skin treatment formulations.