901 results about "Endosperm" patented technology

This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and / or spatially-regulated expression of genes, particularly in plant seeds and related female reproductive tissue. The present invention also provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions comprise novel nucleotide sequences for a seed-preferred promoter known as eep1. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.

A stabilized bran component and a stabilized whole grain wheat flour containing the component are produced by subjecting a bran-enriched coarse fraction which contains bran, germ and starch to grinding or milling to reduce grittiness of the coarse fraction without substantially damaging the starch due to abrasion. The coarse fraction may be stabilized by heating to substantially reduce the lipase and lipoxygenase activity of the coarse fraction without substantial gelatinization of the starch. High levels of natural antioxidants and vitamins are retained while avoiding substantial acrylamide formation during the stabilization. The stabilized coarse fraction or stabilized bran component may be combined with a fine fraction which contains predominantly endosperm to obtain a stabilized whole grain wheat flour. The stabilized whole grain wheat flours and the stabilized bran component exhibit extended shelf life and may be used for making baked goods, such as cookies, with desirable oven spread, and a non-gritty mouthfeel.

The present invention relates to the production of a highly digestible, high protein product (high protein distillers dried grains or high protein DDG) from corn endosperm, and more particularly to a method for the recovery of high protein DDG by using: (i) dehulling and degermination to isolate a low fat, low fiber corn endosperm fraction, (ii) enzymatic hydrolysis to solubilize and alcoholic fermentation to assimilate the starch and non-starch carbohydrates present in the corn endosperm, and (iii) filtration and / or centrifugation to recover the dealcoholized insoluble solids that remain after fermentation of the corn endosperm. The present invention provides an alternative to the traditional dry mill method of processing corn to produce ethanol, and results in the production and recovery of a distillers' by-product (high protein DDG) with increased value and range of use as an ingredient in feeds for farm-raised ruminants and non-ruminants and pet foods. The product of the present invention contains less than about 2.0 weight percent starch, from about 55.0 to about 65.0 weight percent protein, from about 4.5 to about 7.5 weight percent fat, from about 3.0 to about 5.0 weight percent crude fiber, and from about 78.0 to about 90.0 percent total digestible nutrients, and improves the palatability and digestibility of animal feeds and / or pet foods into which it is incorporated, and aids in the management of the health and weight gain of the animal.

The present invention relates to the production of a highly digestible, mid-level protein DDGS from corn endosperm, and more particularly to a method for the recovery of mid-level protein DDGS by using: (i) dehulling and degermination to isolate a low fat, low fiber corn endosperm fraction, (ii) enzymatic hydrolysis to solubilize and alcoholic fermentation to assimilate the starch and non-starch carbohydrates present in the corn endosperm, and (iii) filtration, centrifugation and / or evaporation to recover the dealcoholized insoluble and soluble solids that remain after fermentation of the corn endosperm. The product of the present invention contains less than about 5.0 weight percent starch, from about 40.0 to about 52.5 weight percent protein, from about 4.5 to about 8.5 weight percent fat, from about 3.0 to about 6.0 weight percent crude fiber, and from about 78.0 to about 90.0 percent total digestible nutrients.

The present invention relates generally to an apparatus for and a method of measuring and selecting grain for use in milling, or seed for use in plant breeding. Said method is adapted to optically analyze seeds / grains to qualitatively and quantitatively characterize the seed / grain, and more particularly, to analyze the gradation of color, whiteness, and hard endosperm of the seed / grain. This method and apparatus perform color image analysis of seed / grain sample(s) to characterize multiple quality traits.

Production of recombinant human serum albumin with rice albuminous cell as biological reactor is carried out by taking rice albuminous cell protein as storage site for recombinant protein, taking rice albuminous specific expression starter and signal peptide, entering inductive recombinant human serum albumin into inner-film system of rice serum albumin cell and storing it in rice albuminous protein. It is cheap, has more yield and higher expression.

A process for the production of fresh masa, nixtamalized flour and derived produts is disclosed. Water-lime cooking of pericarp fractions of the corn, and appropriate hydration of the germ and endosperm fractions of the corn is achieved to prepare fresh masa, nixtamalized corn flour and derived products. The pericarp fractions are cooked with lime and water at a temperature between about 50° C. to about 300° C. The germ and endosperm fractions are hydrated with water. The pericarp fractions and the germ-endosperm fractions are milled separately, and the milled pericarp, germ and endosperm fractions are then mixed for producing fresh corn masa. The fresh corn masa can be dehydrated and milled for producing nixtamalizaed corn flour. Also, the pericarp, germ and endosperm fractions can be dried in order to produce nixtamalized corn flour.

The invention discloses a manufacturing method of paraffin sections of zostera marina embryo, comprising the following steps: (1) material drawing and fixation: zostera marina seeds are taken and the fruit skin is stripped, and after endosperm is removed, an integral zostera marina embryo is soaked in FAA (free amino acids) fixing solution and is fixed for more than 48 hours; (2) dehydration: the zostera marina embryo is taken out from the fixing solution and is soaked in alcohol for dehydration; (3) waxing and embedding: paraffin is added gradually in a vessel containing the zostera marina embryo and dimethylbenzene, and conventional paraffin embedding is carried out to obtain the wax blocks containing the zostera marina embryo; (4) slicing, spreading and drying: the wax blocks coated with the zostera marina embryo is fixed on a wheel rotation type slicing machine for carrying out continuous slicing of the paraffin, so as to obtain wax bands and enable the wax bands to be attached to an object slide; (5) dewaxing, rehydration and dyeing of hematoxylin dye solution; (6) slicing, dehydration and dyeing of eosin and alcohol; and (7) transparence and mounting are carried out to manufacture a permanent section. In the manufacturing method disclosed by the invention, the paraffin sections of the zostera marina embryo, which have clear dyeing and an integral texture structure, can be obtained.

Dry milling methods for preparing oat products enriched in the content of β-glucan and methods for preparing foodstuffs incorporating such an enriched oat product especially ready-to-eat cereals are provided. Heat conditioned dehulled oats are dry milled to form a coarse whole non defatted oat flour and then, without a preceding removal of fat, dry fractionated into coarser bran and finer oat flour fractions at multiple stages. The coarse oat flour is first dry classified to separate or form a coarser fraction oat bran containing more concentrated β-glucan and a finer oat flour or starch containing or endosperm containing fraction. The oat bran is subjected to second and third rounds of milling and classification to form a high β-glucan content (>7-9%) oat bran and a low β-glucan content (3%) oat flour. The methods are low cost and commercially practical. Preferably, the high β-glucan content oat bran and oat flour fractions can have a syringic acid to ferulic acid ratio of at least 2.5:1 indicating improved flavor. Preferably, the oat bran and oat flour have a Farinograph value of 5 to 20 minutes indicated partial gelatinization. The oat bran and oat flours can be used to prepare foodstuffs such as ready-to-eat cereals.

Disclosed are skin care compositions exerting a novel complementary dual mode of action in protecting the skin from day-to-day insults. The skin care compositions of the present invention comprise the extract from the liquid endosperm of Cocos nucifera as the principle ingredient along with one or more actives including the fruit rind extracts of Garcinia cambogia, Garcinia indica and Garcinia mangostana, seed coat extract of Tamarindus indicus, Chlorogenic acid extract from the beans of Coffea arabica, seed extracts of Nelumbo species, the leaf extracts of Murraya koenigii and the triterpene pentapeptides of oleanolic acid and ursolic acid. While the extract of Cocos nucifera has been included as a nutritional supplement (a natural cell culture medium) to ensure the maintenance of the viability and constant renewal of the cells of the skin, the other actives have been carefully chosen based on one or more of their effective skin care properties. The compositions of the present invention offer a safe prophylactic / therapeutic management solution to the wide range of pathological states associated with skin insults and the associated psycho-social issues.

The invention discloses a method for preparing stabilized whole wheat flour, which comprises the following steps of: (1) in the process of processing flour, respectively collecting albumen, embryo and bran; (2) performing stabilization treatment on the bran and the embryo by adopting an extrusion method to obtain an extruded material; (3) drying the stabilized material, crushing (sieving by using an 80-mesh sieve) or performing super micron milling (to make the grain size less than or equal to 74 mu m); and (4) uniformly mixing the albumen obtained in the step (1) with the embryo powder and bran powder obtained in the step (3) in a ratio of the albumen to the embryo to the bran collected in the step (1), and packaging to obtain the stabilized whole wheat flour. By the method, lipase activity and fatty acid content in the bran and the embryo can be effectively reduced, the whole wheat flour product keeps the nutrient components of the natural whole wheat, the storage stability is improved, the flour property and processing and edible quality are improved, and the utilization rate and value-added benefit of wheat are improved.

Rice having reduced levels of starch branching enzymes produce grain having a high relative amylose content in the endosperm. The rice grain of this invention can be of a non-shrunken phenotype despite a lesion in the amylopectin synthesis pathway and may be transgenic or nontransgenic.

Rice having reduced levels of starch branching enzymes produce grain having a high relative amylose content in the endosperm. The rice grain of this invention can be of a non-shrunken phenotype despite a lesion in the amylopectin synthesis pathway and may be transgenic or nontransgenic.   1 GCCACCGACA TCCGCCGCAA TGCTGTGTCT CACCTCCTCTTCCTCCTCCG CGCCCGCTCC  61 GCTCCTTCCC TCTCTCGCTG ATCGACCGAG CCCGGGAATCGCGGGCGGGG GTGGCAATGT 121 TCGCCTGAGC GTGGTTTCTT CGCCGCGCCG GTCGTGGCCTGGAAAGGTCA AGACCAATTT 181 CTCAGTTCCT GCGACTGCGC GAAAAAACAA AACCATGGTGACTGTTGTGG AGGAGGTCGA 241 CCACCTTCCT ATATATGATC TGGACCCTAA GTTGGAGGAATTCAAGGATC ACTTCAACTA 301 TAGGATAAAA AGATACCTCG ACCAGAAATG CCTGATTGAAAAACATGAGG GGGGCCTTGA 361 AGAATTTTCT AAAGGCTATT TGAAGTTTGG GATTAATACAGTTGATGGTG CCACAATATA 421 TCGTGAATGG GCGCCTGCTG CACAAGAAGC ACAGCTCATTGGTGAGTTCA ATAACTGGAA 481 TGGTGCAAAA CACAAGATGG AGAAGGATAA ATTTGGCATTTGGTCAATCA AGATTTCACA 541 TGTCAATGGG AAGCCTGCCA TCCCTCACAA TTCCAAGGTTAAATTTCGCT TTAGGCATGG 601 GGGTGGAGCA TGGGTTGATC GTATTCCCGC ATGGATTCGTTATGCAACTT TTGATGCCTC 661 TAAATTTGGA GCTCCATATG ATGGTGTACA CTGGGATCCTCCAGCCTGTG AAAGGTACGT 721 GTTTAAGCAT CCTCGACCTC CAAAACCTGA TGCTCCACGCATCTATGAGG CTCATGTGGG 781 GATGAGTGGT GAAGAGCCAG AAGTAAGCAC ATACAGAGAATTTGCAGACA ATGTGTTACC 841 ACGCATACGG GCAAATAACT ACAACACAGT TCAGTTAATGGCAATCATGG AACATTCCTA 901 CTATGCTTCT TTTGGGTATC ACGTGACAAA TTTTTTCGCAGTCAGCAGCA GATCAGGAAC 961 ACCAGAGGAT CTGAAATATC TTGTTGACAA GGCACATAGTTTAGGATTAC GAGTTCTGAT1021 GGATGTTGTC CATAGCCATG CGAGTAATAA TGTGACCGATGGTCTAAATG GCTATGACGT1081 TGGACAAAAC ACTCATGAGT CTTATTTTCA TACAGGAGATAGGGGCTACC ATAAACTCTG1141 GGATAGTCGT CTGTTCAACT ATGCCAATTG GGAGGTCTTAAGATTTCTTC TTTCTAATTT1201 GAGATATTGG ATGGACGAAT TCATGTTTGA TGGCTTCCGATTTGATGGGG TTACATCAAT1261 GCTATACCAT CACCATGGTA TCAATAAGGG ATTTACTGGAAACTACAAGG AGTATTTCAG1321 TTTGGATACC GATGTGGATG CAATTGTTTA CATGATGCTCGCAAACCATT TAATGCATAA1381 ACTCTTGCCG GAAGCAACTA TTGTTGCTGA AGATGTTTCGGGCATGCCAG TGCTTTGTCG1441 GCCAGTTGAT GAAGGTGGAG TAGGGTTTGA CTTCCGCCTGGCAATGGCCA TTCCTGATAG1501 ATGGATTGAC TACCTGAAGA ACAAAGAGGA CCGCAAATGGTCAATGAGTG AAATAGTGCA1561 AACTTTGACT AACAGGAGAT ATACAGAAAA ATGCATTGCCTATGCCGAGA GCCATGATCA1621 GTCCATTGTT GGTGACAAGA CTATAGCATT TCTCTTGATGGACAAGGAAA TGTACACTGG1681 CATGTCAGAC TTGCAGCCTG CTTCACCTAC CATCAACCGTGGCATTGCAC TCCAAAAGAT1741 GATTCACTTC ATTACGATGG CCCTTGGAGG TGATGGCTACTTAAATTTTA TGGGCAATGA1801 GTTTGGCCAT CCAGAATGGA TTGACTTTCC AAGAGAAGGCAACAACTGGA GCTATGATAA1861 ATGCAGACGT CAGTGGAGCC TTGTCGACAC TGATCACCTTCGATACAAGT ATATGAATGC1921 ATTTGATCAA GCAATGAATG CACTCGAGGA GGAATTTTCCTTCCTGTCAT CATCAAAGCA1981 GATTGTTAGC GACATGAACG AGAAAGATAA GGTTATTGTCTTTGAACGTG GAGATTTGGT2041 TTTTGTTTTC AATTTTCATC CCAACAAAAC TTATAAGGGTTACAAAGTCG GATGTGACTT2101 GCCCGGGAAG TACAGAGTAG CTCTGGACTG TGATGCTTTGGTCTTTGGTG GCCATGGAAG2161 AGTTGGCCAT GATGTGGATC ACTTCACGTC TCCCGAGGGAATGCCAGGAG TACCAGAAAC2221 AAATTTCAAC AACCGCCCTA ACTCATTCAA AGTCCTTTCCCCGCCCCGTA CCTGTGTGGC2281 TTACTATCGC GTTGATGAAG ATCGTGAAGA GCTCAGGAGGGGTGGAGCAG TTGCTTCTGG2341 AAAGATTGTT ACAGAGTATA TCGATGTTGA AGCAACAAGTGGGGAGACTA TCTCTGGTGG2401 CTGGAAGGGC TCCGAGAAGG ACGATTGTGG CAAGAAAGGGATGAAGTTTG TGTTTCGGTC2461 TTCTGACGAA GACTGCAAAT GAAGCATCAG ATTTCTTGATCAGGAGCAAC TGTTGGTGCC2521 CTTGTAATCT GGAGATCCTG GCTTGCCTTG GACTTGGTTGTGGTTCTTTA GCAGTTGCTA2581 TGTACCTATC TATGATATGA ACTTTATGTA TAGTTCGCCTTAAAGAAAGA ATAAGCAGTG2641 ATGATGTGGC CTTAAACCTG AGCTGCACAA GCCTAATGTAAAAATAAAGT TTCAGGCTTT2701 CATCCAGAAT AAAACAGCTG TTCATTTACC ATCTCAAAA

The invention provides a practical technique for breeding dendrobium officinale by using artificial seeds. The invention aims to provide a technique for breeding dendrobium officinale with high efficiency and low cost. The technique comprises the following steps of: inducing dendrobium officinale seeds on a germination culture medium to obtain protocorms, then performing propagation culture on the protocorms, transferring the protocorms to MS culture solution containing 0.5mg / L of ABA to perform shaking culture (100r / min), and replacing the culture solution once every 10 days; selecting the protocorms with length * width of about 0.5-1.5mm * 2.0-3.4mm as embedding propagation bodies by using a 6-mesh nylon mesh screen after 30 days, manufacturing the artificial seeds by adopting an instillation method, preparing basic artificial endosperm from MS, 0.5 mg / L of BA, 0.5mg / L of NAA, 0.3 percent of AC, 3 percent of sodium alginate and 0.3 percent of chlorothalonil, adding 1.5 to 2.0 percent of starch, 1.0 to 2.0 percent of water-retaining agent and 1.0 to 3.0 percent of nano TiO2 into the basic artificial endosperm, preparing artificial seed coat matrix by adding 2 percent of chitosan and 5.0 to 10 percent of nano TiO2 into sodium alginate, and performing germination and storage research of the artificial seeds.

The invention discloses a production method for a paraffin section of a paeonia lactiflora mature embryo. The production method comprises the following steps: drawing materials and fixing: stripping a seed coat, cutting a seed, immersing the seed top part containing the embryo or part endosperm into a FAA stationary liquid, and fixing for more than 24 hours; dehydrating: washing in ethyl alcohol with the concentration of 70% for 3 to 5 times, and performing gradient dehydration from the ethyl alcohol with the concentration of 70%; performing hyalinization: carrying out five level hyalinization by using dimethyl benzene as a transparent reagent; waxing and embedding: pouring out one half volume of dimethyl benzene, adding wax crumbs in a bottle until saturation, staying overnight at the temperature of 40 DEG C; then changing a wax liquid every two hours for three times per day, repeating for three days, and carrying out conventional paraffin embedding; slicing; flattening and bonding: flattening a linoleum tape on an iron plate of a water bath kettle at the constant temperature of 50 DEG C, dewaxing, rehydrating, dyeing through safranine and fast green and performing hyalinization; mounting. According to the invention, the paeonia lactiflora mature embryo paraffin section of which the tissue structure is integrated and clear can be obtained, so that accurate technical support can be provided for researching paeonia lactiflora seed dormancy and germination.

A direct seeding propagation method for Bletilla Striata in greenhouses includes the steps of sorting a seedbed, treating seeds, sowing, raising seedlings, and enhancing the seedlings. The method is simple and easy, low in cost, high in emergence rate, low in season and regional dependency, convenient to popularize, and fully satisfactory to large-area scaled seedling raising. The method has the advantages that problems of seeds are small, with no endosperm, and hardly emerge in the natural conditions are solved, and a new way of fast propagation for plants of Bletilla Striata is developed.

A dry, whole grain hot cereal product which readily hydrates into a creamy, non-gritty, non-pasty, whole grain hot cereal is produced by milling whole cereal grains or combining separate streams or sources of endosperm, bran and germ to obtain a milled whole grain mixture having a particle size distribution of 100% by weight through a No. 16 (1,190 micron), preferably a No. 18 (1,000 micron), most preferably a No. 20 (841 micron) U.S. Standard Sieve, and less than or equal to about 7% by weight through a No. 100 (149 micron) U.S. Standard Sieve. The endosperm, the bran and the germ may be stabilized before or after milling of the whole grains or the endosperm, the bran and the germ, to avoid insect infestation and to at least substantially inactivate lipase and lipoxygenase. The dry, whole grain hot cereal product may be a cook-on-stove or instant hot cereal product.

A stabilized bran component and a stabilized whole grain flour containing the component are produced by subjecting a bran-enriched coarse fraction which contains bran, germ and starch to grinding or milling to reduce grittiness of the coarse fraction without substantially damaging the starch due to abrasion. The coarse fraction may be stabilized by heating to substantially reduce the lipase and lipoxygenase activity of the coarse fraction without substantial gelatinization of the starch. High levels of natural antioxidants and vitamins are retained while avoiding substantial acrylamide formation during the stabilization. The stabilized coarse fraction or stabilized bran component may be combined with a fine fraction which contains predominantly endosperm to obtain a stabilized whole grain flour. The stabilized whole grain flours and the stabilized bran component exhibit extended shelf life and may be used for making baked goods, such as cookies, with desirable oven spread, and a non-gritty mouthfeel.

The invention particularly relates to a breeding method for producing paddy rice with a colorful rice producing function and belongs to the field of special rice breeding. The breeding method comprises the following steps of: (1) selecting a three-line maintenance line B as a female parent to be hybridized with a black rice male parent, carrying out multi-generation backcrossing on the first filial generation and the maintenance line B, and breeding a maintenance line black B with black seed coats and with other properties the same as the maintenance line B; (2) using a sterile line A corresponding to the three-line maintenance line B to be hybridized with the maintenance line black B to produce a sterile line black A containing heterozygous black seed coat genes; (3) using the sterile line black A to be hybridized with a restore line endosperm R to produce first filial F1 generation seeds filial F1; and (4) using the first filial F1 generation seeds filial F1 as production seeds. Thebreeding method has the advantages that hybridized paddy rice seeds capable of being directly used for producing colorful rice can be produced through the breeding method, and the functional colorfulrice can be produced through planting the seeds. The production method is simple, the efficiency is high, the benefits are good, and the produced rice is rich in various nutrition elements.

A method for fractionating whole kernel corn into bran, germ and endosperm fractions. Corn (tempered or non-tempered) is processed through an impact mill and through a screener to divide the initial feed stream into two or more flows or substreams. The substreams or portions thereof may be further processed through a plurality of aspirators, roller mills and screeners to provide bran, germ and endosperm fractions of desired purity levels.

The invention relates to the deeply processing of the agricultural and sideline products especially the dipping in producing method of the corn flour enzyme method. It overcomes the long marinating time, the great water and energy consumption, the SO2 befouling environment existing in the conventional damp producing method of the corn flour. The steps of the producing: firstly, the seed selection corn soak lactobacillus is fermented and cultured and is appended into the corn soak to shorten the time of reaching the effective lactic acid concentration; the corn seed albumen sops to inflate and intenerate, the cell wall is destroyed; secondly, the early dipping corn is cracked into the 4-6 petals to separate the embryo; so the better condition of permeating the lactic acid and the protease is created; thirdly, the vegetable protease is acceded to the cracked corn soak to degrading fully the protein ground substance to releasing the amylum and to shorten the dipping time.

A feed delivery system for a dairy farm wherein a third-party supplier of the system can provide the dairy farm with lists of approved hybrids for the feed components, necessary information inputs for practicing the feed method, and all or a portion of its component needs for the feed ration corresponding to a feed ration that contains: at least one primary forge source selected from the group consisting of brown midrib corn silage, dual-purpose corn silage, leafy corn silage, and grass silage; a secondary forage source selected from the group consisting of dual-purpose corn silage, alfalfa haylage, alfalfa dry hay, grass silage, and alfalfa / grass mix; a corn grain of floury and / or vitreous endosperm starch grain into which normal dent corn or mutt corn may be blended in order to achieve a predetermined level of in vitro starch digestibility; such grain component being further processed to produce a specific particle size of the blended starch. The feed delivery system can also provide the dairy farm with real-time characterizations of at least some of its feed ingredients, and re-penning strategies for maximizing the milk productivity of the cows.

A technology for processing the highland barley includes such steps as washing highland barley grains, removing impurities, wetting, peeling, screening to obtain the peel and grains, preparing the instant peel powder which contains protein, unsaturated fatty acid, edible fibers, vitamins, minerals and beta-glucosan, and refining the grains, which can be used to cook staple food.

The invention aims at the characteristics of complicated process, high energy consumption and high pollution in wet degerming of the existing method of corn deep processing and provides a steam explosion technology for degerming corn and a poly-generation technology for deeply processing corn endosperm.Corn kernals are processed by the steam explosion technology, so that the separation among the endosperm, embryo and testa can be realized. After steam explosion, the corn endosperm is extracted by ethanol and then is distilled, precipitated and the like, so that alcohol-soluble protein and uranidin product can be respectively obtained. After the alcohol-soluble protein and the uranidin of the endosperm are extracted, the endosperm can be used for fermenting the ethanol, and the fermentation effect of the endosperm is slightly better that that of corn endosperm prepared by the wet degerming; furthermore, the step of gelatinization before saccharification and liquidation can be omitted, so that the energy consumption is reduced.

The present invention provides a nixtamalization process wherein corn endosperm fractions, subfractions, or combinations thereof are individually nixtamalized to produce food products. The invention also comprises food products, including masa, instant masa, tortillas, and tortilla-related food products produced by the present invention's methods, including food products that have controllable fat and / or fiber levels.

Production of malt is carried out by immersing for wheat grain, germinating and drying. It can supply sufficient hydrolase for saccharification to decompose protein into soluble nitrogen; it can supply alpha-amino-nitrogen and dissolve albuminous cell wall to degrade starch grain. It's controllable, has better physical-bio-chemical reaction and quality.

The invention discloses a transgenic breeding method producing astaxanthin in crop seed endosperm. The transgenic breeding method specifically includes the steps of S1, optimizing the sequence of a PSY gene, a CrtI gene, a BHY gene and a BKT gene according to the codon preference of a breeding crop, and respectively fusing the four genes with the optimized sequences with breeding crop endosperm expression promoters and transit peptides to build gene expression boxes; S2, building the four gene expression boxes obtained in the S1 into plant transformation vectors to obtain the plant transformation vectors of the PSY, the CrtI, the BHY and the BKT; S3, using the plant transformation vectors obtained in the S2 to transform the breeding crop to obtain a transgenic crop with the salmon-colored endosperm and synthesized astaxanthin. The transgenic breeding method has the advantages that the astaxanthin can be synthesized in the seed astaxanthin of crops (especially paddy rice), the crops can be directly used for eating and also be used as the raw materials for producing the astaxanthin, and the method has important guiding significance and production application value to the novel functional crop breeding.

Production of recombinant human serum albumin with rice albuminous cell as biological reactor is carried out by taking rice albuminous cell protein as storage site for recombinant protein, taking rice albuminous specific expression starter and signal peptide, entering inductive recombinant human serum albumin into inner-film system of rice serum albumin cell and storing it in rice albuminous protein. It is cheap, has more yield and higher expression.