87 results about "Endosperm" patented technology

The application provides a rice heart-white gene WCG1 and application thereof, and particularly relates to positioning, cloning, function verification and application of a WCG1 gene for controlling a rice kernel heart-white phenotype. A C / A natural variation exists at a ninth exon 986 site of the WCG1 gene, the variation site makes the rice kernel present a heart-white phenotype, that is, a kernel middle heart-white and edge transparent phenotype, and the phenotype is an excellent phenotype of "sake brewing good suitable rice" specially selected in sake brewing.

The invention discloses a breeding method and application of fragrant soft rice two-line hybrid rice with an off-white anti-counterfeiting mark, and belongs to the technical field of rice breeding. According to the method, soft rice genes with recessive grey white endosperm markers, genic male sterility character genes and the like are subjected to synchronous phenotype aggregation directional selection for the first time, the problem that the genic male sterility character and excellent taste quality character are difficult to synchronously select in the earliest segregation generation is solved for the first time, and in addition, the technical link of taste quality detection and identification can be saved; the selection efficiency is improved; meanwhile, the problem that the taste quality and control genes thereof need to be detected and identified for multiple times during mating and parent selection of high-quality hybrid rice is solved for the first time; besides, the soft rice character gene with the recessive grey white endosperm marker is synchronously introduced into a two-line hybrid rice parent, through main target character aggregation response identification, the fragrant soft rice two-line hybrid rice with the grey white marker and the aromatic flavor is successfully created for the first time, the yield is increased, and infringement and loss of independent brand fragrant rice products can be prevented.

The invention relates to low-damage endosperm rice and a preparation process thereof. Specifically, the invention provides a brown rice milling method, the method comprises the steps of unhusked rice opening and fine milling, and the method comprises the step of controlling the protein retention rate, the damaged starch content and the protein-to-ash ratio of milled brown rice to be in the following ranges: the protein retention rate is 88.0% or above, the damaged starch content is 0.480% or below, and the ash-to-protein ratio is 0.055 or above.

The invention belongs to the field of plant engineering technology and crop germplasm resource innovation, and particularly relates to a rice BEIIb gene mutant, application of the rice BEIIb gene mutant in improving rice resistant starch and an improving method. A mutant material with high resistant starch content is created by knocking out the rice BEIIb gene, and compared with a wild type, mutant seeds show an obvious chalky phenotype, composite starch particles are abnormal in a spherical or ellipsoidal shape, the amylose content is remarkably increased, the protein content is remarkably reduced, the resistant starch content is remarkably increased, and the resistant starch content is remarkably reduced. The hardness of cooked rice is remarkably increased, and the elasticity is remarkably reduced. Through transcriptomics analysis, the protein mainly participates in biological pathways such as carbohydrate metabolism, chloroplast photosynthesis, calmodulin combination and secondary metabolite synthesis, and the expression level of endosperm starch synthesis related genes is obviously changed. The method can provide theoretical basis and method reference for breeding of high-quality rice varieties with high resistant starch.

The invention discloses an efficient corn germ extracting machine which comprises a bottom plate, two mounting rods are fixedly mounted at the top of the bottom plate, a same fixing plate is fixedly mounted at the tops of the two mounting rods, a hydraulic cylinder is fixedly mounted at the bottom of the fixing plate, and a follower plate is arranged below the hydraulic cylinder; and a filter basin is arranged below the follower plate. The hydraulic cylinder drives the driving plate and the follow-up plate to synchronously move downwards, so that the follow-up plate is tightly attached to the top end of the filter basin to achieve sealing of the top end, dust overflowing and material splashing are effectively avoided, then the hydraulic cylinder continues to push the driving plate to move downwards to start the servo motor, the rolling block rotates at a high speed while swinging and rolling, and the dual treatment effect is achieved; the separation efficiency and purity of corn germs and endosperm are greatly improved, in the treatment process, dust and chippings in the filter basin can be sucked and collected, dust harm is reduced, finally, a hydraulic cylinder reversely drives a driving plate to move upwards, back flushing cleaning is conducted on the inner wall of the filter basin, and residual material accumulation is avoided.

[Problem] To provide a composition for regulating blood pressure for a healthy person. [Solution] A blood-pressure-regulating composition for a healthy person, said composition being characterized by containing a guar gum degradation product, and being characterized in that: 70 mass% or more of the guar gum degradation product has an average molecular weight within the average molecular weight range of 1.0 × 103 to 1.0 × 105; the composition has a viscosity of 10 mPa·s or less when a 2 mass% aqueous solution at 37°C is measured at 10 rpm using an E-type viscometer, or has a viscosity of 5 mPa·s or less when the aqueous solution is measured at 20 rpm, or has a viscosity of 3 mPa·s or less when the aqueous solution is measured at 30 rpm; the composition is obtained by hydrolyzing a galactomannan polysaccharide which contains guar-derived endosperm and in which the content ratio (galactose:mannose) of galactose and mannose is within the range of 1:1.3 to 1:2, by using a microorganism-derived β-mannanase to reduce the molecular weight of the polysaccharide; and the composition contains at least 70 mass% of dietary fiber in terms of the dietary fiber content stipulated in the enzyme-HPLC method.

The invention belongs to the technical field of plant genetic engineering and molecular breeding, and particularly relates to a new function and application of a transcription factor gene OsMYB87 derived from rice, an OsMYB87 function deletion mutant created through a gene editing technology and application of the OsMYB87 function deletion mutant in increasing the rice grain length and grain weight. The OsMYB87 gene found in the invention shows a key differentiated characteristic: preliminary phenotypic observation shows that the phenomenon that chalkiness of endosperm is remarkably aggravated inevitably due to OsMYB73 knockout is not observed while the function deficiency of the OsMYB87 gene brings a remarkable yield increasing effect (grain length and grain weight increase). The OsMYB87 is strongly prompted to be possibly located on a different regulatory pathway, and as a breeding target, the OsMYB87 provides greater possibility and a better potential choice for maintaining the appearance quality of rice commodities while realizing yield increase, so that the OsMYB87 has higher application value.

The invention relates to a method for creating a rice material with low absorbable protein content and remarkably thickened endosperm cell walls and application of the rice material. Specifically, the invention provides a method for improving rice, and improved rice with unique characters is obtained by down-regulating the expression of glutelin genes and down-regulating the expression of globulin genes. In the improved rice, the content of absorbable protein is low, and endosperm cell walls are remarkably thickened. Besides, the improved rice is low in in-vitro digestion rate, has the potential postprandial blood sugar control effect and is suitable for preparing auxiliary food for treating chronic kidney disease patients, diabetic patients and the like.

The application discloses a kind of high altitude permafrost engineering disturbance area's vegetation recovery method, comprising the following steps: to meet sowing time period, turn over, supplement grass seed, sowing time period is: deep 0 cm place consecutive 10 days daily average ground temperature value is not less than first preset temperature value and deep 50~60 cm place consecutive 12 daily average ground temperature value is not less than second preset temperature value after the deadline 3~10 days;Cover multilayer covering net, until deep 0 cm place consecutive 8 days average ground temperature value is not less than third preset temperature value, remove part of covering net, only remain a layer of covering net;When grass seedling grows to 5~8 cm, remove last layer of covering net.The application is limited by the daily average ground temperature value of deep 0 cm in frozen soil disturbance area and deep 50~60 cm in frozen soil disturbance area, which helps to keep the endosperm cells inside active after seed sowing, increases the germination rate of grass seeds, and has good vegetation recovery effect.

The present application belongs to the technical field of coffee making, and particularly relates to a low-temperature extraction method of coffee liquid. The low-temperature extraction method of coffee liquid comprises the following steps: (1) peeling coffee fresh fruits to retain endocarp, endosperm and embryo, and obtaining peeled coffee beans; (2) adding water to the peeled coffee beans, sterilizing, then adding fermentation inoculum to obtain fermentation material, and carrying out anaerobic fermentation to obtain fermentation liquid; (3) after the fermentation is completed, sterilizing, drying, crushing to obtain coffee powder, mixing the coffee powder with water, then carrying out pressure treatment, filtering to obtain coffee extraction liquid and coffee residue; (4) drying the coffee residue, adding the residue into an extraction kettle to carry out supercritical carbon dioxide extraction, obtaining extraction, filtering to obtain extraction product; (5) mixing the coffee extraction liquid and the extraction product to obtain coffee liquid. The coffee liquid of the present application has suitable content of gallic acid, high antioxidant property, excellent taste and aroma.

The invention discloses an extraction and separation process of barley endosperm, and belongs to the field of grain extraction. The method specifically comprises the following steps that barley grains are cleaned and smashed in spring, then classified screening, hulling and sorting are conducted, pretreated endosperm powder is obtained through grinding, endosperm separation and drying are conducted, complete barley grains and broken or defective barley grains can be separated through classified screening, and reduction of loss of raw materials in the subsequent hulling and sorting process is facilitated. A separation accelerant is added in the endosperm separation process, calcium ions can react with hydroxyl contained in starch molecules to form a complex structure, the stability of starch particles is enhanced, the sugar alcohol compound can inhibit gelling of the starch molecules, especially amylose, through the competitive effect, the recovery rate of starch in barley endosperm is increased, and the yield of the barley endosperm is increased. And the centrifugal liquid obtained by separation in the separation process enhances the recovery of the by-product beta-glucan under the action of the selective adsorbent, reduces the influence of impurity molecules, and improves the recovery rate and purity of the beta-glucan.

The present application relates to the technical field of microorganism or culture thereof processing, and particularly relates to a method for breeding pleurotus ostreatus mycelium by using highland barley as a substrate. The method is as follows: highland barley is crushed and sieved, then water is added to prepare highland barley slurry, and a complex enzyme system composed of amylase and cellulase is added to perform liquid enzymolysis to obtain an enzymolysis substrate; lactic acid bacteria strains are inoculated into the enzymolysis substrate to perform solid-state fermentation to obtain a fermentation substrate; the fermentation substrate is subjected to moist heat sterilization treatment to obtain a sterilized substrate; the sterilized substrate is inoculated with pleurotus ostreatus original seeds, and is placed in a culture environment for culture to obtain pleurotus ostreatus mycelium. The scheme breaks the physical barrier of the fiber network of the highland barley epidermis and the starch crystal structure of the endosperm by using the complex enzyme system and lactic acid bacteria fermentation, and the substrate is pre-degraded before the mycelium is inoculated, so that the mycelium can directly absorb free carbon sources, the germination time is shortened, the daily growth rate is improved, the residual sugar rate of the substrate is reduced, and the dry weight biomass of the mycelium is increased.

The invention discloses a breeding method and application of fragrant soft rice three-line hybrid rice with an off-white anti-counterfeiting mark, and belongs to the technical field of rice breeding. According to the method, the problem that the taste quality and control genes thereof need to be detected and identified for multiple times during parent selection of high-quality hybrid rice is solved for the first time, consistency of the taste quality and the control genes thereof of the hybridized and matched parents is ensured, and the breeding cost is reduced; synchronous phenotype polymerization selection is carried out on an off-white endosperm marker gene representing excellent taste quality and a high combining ability, aromatic flavor and maintenance gene, so that the problem that taste quality selection is difficult to carry out on a high-quality hybridization maintenance single plant in a separation early generation is solved for the first time; in addition, soft rice marker genes with recessive grey-white endosperm are synchronously introduced into three-line hybrid rice parents, through main target character aggregation response identification, the fragrant soft rice three-line hybrid rice with grey-white markers in rice grains is successfully created for the first time, the yield is increased, and consumers can conveniently identify the authenticity of products and make brands.

The invention discloses a Saccharomyces mirabilis fermentation broth and a preparation method and application thereof, and the preparation method comprises the following steps: crushing or homogenizing coconut solid endosperm to prepare a fermentation medium; inoculating saccharomyces cerevisiae to the fermentation culture medium for fermentation; and after the fermentation is finished, carrying out separation treatment to obtain the saccharomyces cerevisiae fermentation liquor. Compared with the prior art, the saccharomyces mirabilis is selected as a fermentation strain, the coconut solid endosperm is taken as a main fermentation substrate, the metabolic characteristics of the strain are fully exerted under a mild fermentation condition, and the mannan in the coconut solid endosperm is efficiently converted into mannan oligosaccharide; the obtained saccharomyces cerevisiae fermentation liquor has excellent moisturizing and repairing effects and the like, and has a good application prospect in cosmetics.

The application relates to the technical field of eye drops, in particular to an eye nursing liquid containing stem cells and a preparation method thereof, which comprises physiological saline, stem cells, punicalin, porous adsorption material and traditional Chinese medicine extract according to mass parts, the traditional Chinese medicine extract comprises psyllium, atractylodes, radix falcaliae, cistanche, stone lotus, radix paeoniae and poria cocos, and the porous adsorption material is obtained by swelling and drying treatment of solid endosperm of a coconut. The application repairs eye injuries through stem cells, reduces cell apoptosis and inflammatory reactions, promotes blood vessel regeneration and improves the repair ability of eye tissues; the natural porous adsorption material is used as a carrier to adsorb the effective components such as traditional Chinese medicine extract and stem cells on the eyes, prolongs the drug residence time, has a moisturizing effect at the same time, and relieves the dry eye symptoms; the eye nursing liquid does not contain preservatives and has a longer sustained effect after use.

[Problem] To provide a composition for controlling blood amino acid or gastrointestinal hormone concentration after ingesting food and drink. [Solution] This problem is solved by a composition for controlling blood amino acid or gastrointestinal hormone concentration, said composition being ingested in order to control blood amino acid or gastrointestinal hormone concentration after ingesting food and drink, and being characterized by containing a guar gum decomposition product and by containing at least 70 mass% of the dietary fiber content specified according to an enzyme-HPLC method. 70 mass% or more of the guar gum decomposition product contained in the composition has a number average molecular weight of 1.0×103 to 1.0×105. By using an E-type viscometer, the viscosity of the guar gum decomposition product at 37°C is found to be 10 mPa∙s or less when a 2 mass% aqueous solution is measured at 10 rpm, to be 5 mPa∙s or less when the same is measured at 20 rpm, or to be 3 mPa∙s or less when the same is measured at 30 rpm. The guar gum decomposition product contains guar-derived endosperm. Additionally, the guar gum decomposition product was obtained by using microorganism-derived β-mannanase to hydrolyze a galactomannan polysaccharide in which the content ratio of galactose and mannose (galactose:massnose) is 1:1.3 to 1:2, and then low-molecularizing the resultant.

The utility model relates to rice processing technical field, concretely is energy -saving type rice milling device of double -deck structure, including primary rice milling device body, the secondary rice milling mechanism is welded to primary rice milling device body downside, the blow mechanism is set to the rear side of secondary rice milling mechanism lower end discharge gate, the collection box is set to the secondary rice milling mechanism discharge gate downside, the impurity tank is set to the front side of collection box, the blow mechanism includes the blow box, the fan of being installed to a plurality of blow box interiors, the limiting plate of being welded to the left and right ends of blow box front side surface and the air flue of being welded to the left side surface rear end of blow box, the first air inlet pipe is set to the air flue upper surface middle position. The utility model sets up a secondary rice milling mechanism in primary rice milling mechanism body downside in the prior art, after the primary rice milling mechanism carries out the preliminary shelling to rice, the shelled rice enters the secondary rice milling mechanism and removes the skin layer, and the endosperm is directly produced in primary, so as to improve the edible quality of rice.

The application discloses a breeding method for synthesizing resveratrol and pterostilbene in rice seed endosperm, and relates to the technical field of biology.The transgenic breeding method comprises the steps of transforming a recombinant carrier into rice callus and then cultivating the rice callus into a transgenic rice plant; the recombinant carrier comprises a gene expression cassette for expressing VvSTS protein, a gene expression cassette for expressing VvROMT protein, a gene expression cassette for expressing At4CL protein and a gene expression cassette for expressing transcription factor OsMYBP1 protein.The application realizes the synthesis of resveratrol and pterostilbene in rice endosperm, and the product can be used as raw material for producing resveratrol and pterostilbene, and has important guiding significance for new functional breeding.

The present invention relates to a recombinant vector for genome editing in rice. The vector comprises of GluB1 promoter of rice along with Cas 12 a gene and a guide RNA. Editing the targeted gene within localized tissue effectively mitigates the pleiotropic effect observed in non- targeted tissues, thus reducing the potential for unintended consequences. Tissue-specific genome editing (TSGE) plays a pivotal role in alleviating the metabolic burden on plants by restricting modifications to specific areas. The designed CRISPR-ESGE binary construct was introduced into Agrobacterium, which was then used to infect rice callus derived from seeds. The regenerated transformed plants were confirmed to contain the Cas12a gene, and the endosperm-specific expression of Cas12a was validated through RT-PCR analysis. Genome editing (GE) utilizing Cas12a offers distinct advantages over Cas9, which is characterized by heightened target specificity and decreased propensity for off-target effect, thereby potentially enhancing the grain starch content.

The application belongs to the technical field of corn genetic breeding, and specifically discloses a corn kernel development gene ZmCHR24 and application thereof. ZmCHR24 The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The application obtains a mutant material with loss of gene function through EMS mutagenesis and Mu transposon insertion technology. ZmCHR24 The phenotype analysis result shows that, compared with the wild type, ZmCHR24 the kernel of the mutant with loss of gene function presents severe development defects, and specifically, the kernel is only pericarp with no embryo and endosperm. ZmCHR24 The above result shows that the gene is a key gene for regulating corn kernel development. The application provides an important theoretical basis and gene resource for genetic improvement and molecular design breeding of the yield trait of corn kernels.

A sugar replacing composition has no traditional sweeteners that contain novel ingredients with added health benefits and provides sweetness characteristics of sugar without the aftertaste of sweeteners and without the glycemic load of sugar. Specifically, the sugar replacer composition includes digestion resistant soluble fiber comprising an oligosaccharide matrix of glucose and / or fructose and / or xylose oligomers with digestive resistant properties, along with a freeze dried extract from the endosperm of the coconut fruit and / or a freeze-dried extract from the pulp of the banana fruit, where the ingredients contribute to the added health benefits and the sweetening capacity of the composition.

The invention discloses a breeding method for synthesizing resveratrol and pterostilbene in rice seed endosperm, and relates to the technical field of biology. The transgenic breeding method comprises the following steps: transforming a recombinant vector into a rice callus, and culturing into a transgenic rice plant, the recombinant vector comprises a gene expression cassette for expressing VvSTS protein, a gene expression cassette for expressing VvROMT protein, a gene expression cassette for expressing At4CL protein and a gene expression cassette for expressing transcription factor OsMYBP1 protein. The synthesis of the resveratrol and the pterostilbene in the rice endosperm is realized, and the product can be used as a raw material for producing the resveratrol and the pterostilbene, and has important guiding significance for novel functional breeding.

This invention discloses a method for detecting lipids in the embryo and endosperm of plant seeds, comprising the following steps: (1) separating the embryo and endosperm from the plant seeds; (2) freeze-drying the embryo and / or endosperm to remove free water; (3) grinding the embryo and endosperm into powder, extracting the embryo or endosperm powder with a first organic solution containing 2,6-di-tert-butyl-p-cresol (BHT), drying the extract to obtain a lipid extract, suspending the lipid extract in a second organic solution to obtain an LC-MS sample; (4) analyzing the sample using a liquid chromatography-mass spectrometry (LC-MS) instrument, processing the data, and determining the content of each lipid compound. The lipid composition of the embryo and endosperm obtained by this invention is accurate and reliable, and can be used for the evaluation of high-oil new germplasm.

The invention discloses a method for preparing a pure plant-based coconut milk beverage from mixed bean pulp, and belongs to the technical field of plant-based food processing. The method comprises the following steps: performing high-temperature drying on mixed beans, performing slight crushing, and removing bean hulls and germs to obtain bean endosperm; mixing the bean endosperm with boiling water, pulping and filtering to obtain raw soybean milk; sterilizing the raw soybean milk to obtain a soybean milk base material; hydrolyzing the soybean milk with protease under specific conditions to further improve flavor and protein functional characteristics; and then mixing the enzymolysis soybean milk with ingredients such as freshly squeezed coconut meat juice and cane sugar, and performing high-speed shearing, homogenization and UHT sterilization to prepare the pure plant-based coconut milk beverage.

The application discloses a corn germplasm resource viability cross-germplasm discrimination method, system, device and medium, relates to the corn viability discrimination technical field, and the method obtains the hyperspectral data of the embryo region and the hyperspectral data of the endosperm region; and obtains the hyperspectral data of each sample corn seed; each hyperspectral data is pretreated; each pretreated hyperspectral data is subjected to feature selection, and the constructed double-branch spectral Transform corn germplasm cross-germplasm viability detection model is trained in combination with the viability label data of each sample corn seed; the trained double-branch spectral Transform corn germplasm cross-germplasm viability detection model is used to discriminate the cross-germplasm corn to be discriminated, and the viability prediction result of the cross-germplasm corn to be discriminated is obtained, and the application can significantly improve the accuracy and reliability of cross-germplasm corn germplasm resource viability discrimination.

The invention relates to the technical field of flour processing, in particular to low-temperature multi-stage grinding equipment for flour processing, which comprises a discharging bin and a conveyor, a flour multi-stage grinding mechanism capable of performing low-temperature multi-stage grinding on wheat is mounted on the conveyor, bran is conveyed into a second peel mill, coarse powder is conveyed into a core mill, flour is conveyed into a collecting bin, and the collecting bin is used for collecting wheat bran. The core grinding machine grinds coarse powder into flour and transports the flour into the collecting bin, the second peel grinding machine scrapes off residual endosperm from bran one by one to produce the flour, so that the bran is cleaner and cleaner, and finally the flour is transported into the collecting bin, and wheat multi-stage grinding can maximally separate wheat peels and germs through layer-by-layer endosperm stripping and scraping; and the temperature controller can control the internal temperature of the flour mill, so that the flour mill, the flour mill and the flour mill are kept in a low-temperature environment, the situation that the quality of the flour is affected due to too high temperature is avoided, and low-temperature multi-stage grinding of the flour is achieved.

The invention discloses a gene related to synthesis of amygdalin from crop seed endosperm and application of the gene, and belongs to the technical field of gene engineering. According to the invention, gene sequences of CYP79D16, CYP71AN24, UGT94AF3 and UGT94AF2 are optimized, CYP79D16 and CYP71AN24 are connected by using a linker peptide I to obtain a fusion gene I, and the fusion gene I is fused with a breeding crop endosperm strong expression promoter to construct a gene expression cassette I; connecting UGT94AF3 and UGT94AF2 by using a connecting peptide II to obtain a fusion gene II, and fusing the fusion gene II with a breeding crop endosperm strong expression promoter to construct a gene expression cassette II; transferring the gene expression cassette into an expression vector to construct a recombinant vector, and introducing the recombinant vector into a plant to obtain a transgenic crop. According to the invention, synthesis of amygdalin in crop seed endosperm is realized, the storability of crop seeds is improved, and the product can be used as a raw material for producing amygdalin, and has guiding significance and production application value for crop breeding.

The invention is suitable for the technical field of agriculture, and provides a fruit quality grading method and system based on a multi-modal model, which improves the grading precision and universality by constructing a multi-modal and multi-scale feature fusion system, directly obtains accurate physicochemical index parameters such as sweetness, pH, vitamin C concentration and the like, avoids spectrum inversion errors, and improves the quality of fruits. And an appearance and internal quality combined judgment basis is formed in combination with a double-view-angle image. Meanwhile, the cross-modal interaction relation is dynamically modeled, the modal weight is adaptively distributed, and the redundancy problem of an existing fusion means is solved. In addition, global attributes and local details are synchronously captured through a pyramid structure, fruits with large size differences are compatible, and the grading performance and compatibility of the system are remarkably improved. A hierarchical model is deployed in built-in edge computing hardware of fast detection equipment, and a full-link localization closed loop of data acquisition, feature extraction, hierarchical discrimination and result display is completed. Network dependence is eliminated, off-line real-time detection is supported, and the operation and maintenance cost is greatly reduced.

The invention discloses a creation method of 2-phenethyl alcohol biosynthetic rice with a rose fragrance type characteristic. The creation method comprises the following steps: taking an mDHS1 gene, a PAAS gene and a PAR gene to construct a pRHVc-mDHS1-PAAS-PAR recombinant plasmid, transforming the pRHVc-mDHS1-PAAS-PAR recombinant plasmid into a callus through an agrobacterium tumefaciens-mediated method, and carrying out screening and plant regeneration of resistant calluses to obtain rice with overexpressed mDHS1, PAAS and PAR genes. According to the rice, 2-PE can be effectively synthesized in rice endosperm. Based on a synthetic biological technology and metabolic engineering, the pRHVc vector is used for overexpressing mDHS1, PAAS and PAR genes in rice endosperm through a transgenic means, 2-PE is efficiently and directionally synthesized and stably enriched in rice grains, and the economic added value of rice crops is remarkably increased.

The invention discloses a gene OsGluTRBP related to rice endosperm powder as well as an encoded protein and application thereof, and the gene OsGluTRBP provided by the invention is a DNA (Deoxyribose Nucleic Acid) molecule as shown in 1), 2), 3) or 4): 1) a DNA molecule as shown in SEQ ID NO.1; 2) a DNA molecule as shown in SEQ ID NO. 2; 3) a DNA molecule which is hybridized with the DNA sequence limited by 1) or 2) under strict conditions and is used for coding the protein; and 4) a DNA molecule which has more than 90% of homology with the DNA sequence limited by 1) or 2) or 3) and is used for coding the plant endosperm development related protein. The invention also provides a protein coded by the gene, the protein affects the development of plant endosperm, and a transgenic plant with normal endosperm development can be cultivated by introducing the coding gene of the protein into a plant with abnormal endosperm development. The protein and the coding gene thereof can be applied to plant genetic improvement. The coding gene of the protein is knocked out in a wild type to obtain an endosperm powder plant with endosperm development defects.