200 results about "Endosperm" patented technology

The invention discloses a wheat grain protein spatial distribution difference identification method. The method comprises the following steps: S1, constructing a semantic segmentation data set; an improved U-Net network is adopted as a model architecture, and preliminary background removal is completed; s2, binarization processing is carried out, and preliminary segmentation is completed; obtaining an endosperm edge contour by using a Sobel edge detection algorithm; determining the center position of an endosperm area; carrying out layer-by-layer corrosion to obtain a radial layered structure image; s3, acquiring a sampling matrix; a K-means + + algorithm is adopted to initialize the clustering center for iteration; and identifying the protein and calculating the space distribution proportion of the protein. According to the method, semantic segmentation of the U-Net model, dynamic layering of radial corrosion and component clustering of the K-means + + algorithm are improved, full automation from image preprocessing to protein quantitative analysis is achieved, tedious operation of manual description is reduced, image processing is more accurate, layering processing can be conducted on the image, and the protein distribution gradient can be accurately reflected.

System for and method of producing a pure starch slurry and alcohol by using combined corn wet and dry milling processes. A simple and lower cost process to obtain starch inside floury endosperm is provided. The starch can be highly purified to meet green technology process needs. The remaining starch inside the corn kernels can be used as feedstock for alcohol production and to produce valuable co-products such as oil and various animal feeds.

The invention discloses a corn germplasm resource viability cross-germplasm discrimination method, system, equipment and medium, and relates to the technical field of corn viability discrimination, in the method, hyperspectral data of an embryo area and hyperspectral data of an endosperm area are obtained; obtaining hyperspectral data of each sample corn seed; each piece of hyperspectral data is preprocessed; feature selection is carried out on each piece of preprocessed hyperspectral data, and a constructed double-branch spectrum Transform corn germplasm cross-germplasm viability detection model is trained in combination with viability label data of each sample corn seed; the trained double-branch spectrum Transform corn germplasm cross-germplasm viability detection model is utilized to discriminate the cross-germplasm corn to be discriminated, the viability prediction result of the cross-germplasm corn to be discriminated is obtained, and the accuracy and reliability of cross-germplasm corn germplasm resource viability discrimination can be remarkably improved.

The invention relates to the technical field of plant genetic engineering, and particularly provides a rice endosperm tissue specific promoter pPROLM26 and application thereof. According to the invention, a tissue-specific promoter with a nucleotide sequence as shown in SEQ ID NO.1 is separated from a rice alcohol-soluble protein gene, the tissue-specific promoter is cut off for four times, and then the cut-off promoter fragment with an endosperm-specific promoter function as shown in SEQ ID NO.2-5 is obtained after molecular identification is carried out on the cut-off promoter fragment and an RUBY visual marker; the betaine content of the expression product can reach 2.5% of the dry weight of the seeds through determination, and the method has very high application value in the field of plant synthetic biology. The invention further discloses application of the promoter or the truncated promoter fragment in the aspects of improving crop seed quality, improving crop traits, cultivating new varieties of transgenic plants by using key elements of the promoter or expressing high value-added proteins by using a seed bioreactor and the like.

The invention provides a wheat gene sample automatic extraction device, which comprises an extraction processing box, supporting foot bases, a sealing door, an automatic control panel group, a vibration crushing box, a cooling sealing cover, an insulating handle, a cooling liquid nitrogen tank and an analysis detection box, the supporting foot bases are respectively in bolted connection with four corners of the lower part of the extraction processing box; the sealing door hinge is connected to the lower side of the front part of the extraction processing box; the automatic control panel set is connected to the upper side of the front portion of the extraction processing box through screws. Liquid nitrogen grinding is combined with ceramic grinding beads, and a vibration motor, a supporting tray, a locking screw, a test tube clamping seat and a constraint clamping hole are utilized, so that high-throughput treatment of multiple wheat samples can be realized at the same time, the DNA extraction cost of a single sample is reduced by 60%, and cells are uniformly broken; and the analysis detection box, the detection probe group, the cracking bin, the communicating pipe and the processor are arranged, so that different tissues such as wheat endosperm (high starch) and leaves (high fiber) can be processed.

The present invention relates to the field of plant genetic engineering technology, specifically providing a rice endosperm tissue-specific promoter, pPROLM26, and its applications. The present invention isolates a tissue-specific promoter with the nucleotide sequence shown in SEQ ID NO. 1 from a rice alcohol-soluble protein gene. This tissue-specific promoter is truncated four times and then molecularly identified with a RUBY visualization marker to obtain truncated promoter fragments shown in SEQ ID NOs. 2 to 5, each with endosperm-specific promoter function. The expression product has been measured to contain up to 2.5% betaine of the seed dry weight, demonstrating its high application value in the field of plant synthetic biology. The present invention further discloses applications of the promoter or truncated promoter fragments in improving crop seed quality, improving crop traits, cultivating new transgenic plant varieties using key elements of the promoter, or expressing high-value-added proteins using seed bioreactors.

Provided is a gene ZmFIE2 for regulating maize kernel development, an encoded protein thereof, an InDel molecular marker, and a use thereof, aiming to solve the current technical problems of low maize haploid induction rate, long breeding cycle, and low maize yield. An InDel molecular marker for detecting ZmFIE2 mutants was screened out, and a primer for InDel molecular marker was designed, the InDel molecular marker and the primer thereof can be applied to the breeding of maize kernel size varieties and maize haploid or diploid lines. From the epigenetic level, it was found that ZmFIE2 regulates the development of maize embryo and endosperm, affects the accumulation of kernel storage materials, and affects the cell cycle process of endosperm cells, which has great value in improving maize yield. By using multiple created ZmFIE2 allele mutants, research has found that the loss of ZmFIE2 function can directly determine the division of endosperm cells and apomixis, which will provide new gene resources and new ways to achieve high-frequency haploid induction and heterosis fixation in maize.

The invention relates to a corn ZmmtRF2a gene mutation marker, an identification primer and application of the corn ZmmtRF2a gene mutation marker in regulation and control of grain development. A natural grain mutant crk5 found in a corn field breeding line selection process is utilized, a target gene ZmmtRF2a causing crk5 grain mutation is obtained through phenotypic analysis, linkage analysis and map-based cloning technologies, the gene encodes a peptide chain release factor 2a for mitochondrial localization, the peptide chain release factor 2a affects the mitochondrial function, and the gene can be used for inducing the mutation of the crk5 grain. The development of corn embryos and endosperm and the accumulation of grain storage substances are regulated and controlled. In crk5, ZmmtRF2a lacks 5th to 7th exons, the encoded truncated protein does not have GGQ and SPF motifs, the motifs are crucial to release activity of RF, mitochondrial binding and termination codon recognition, technical support is provided for utilization of the gene, and a new way is opened up.

This invention discloses a rice starch synthesis-related gene, OsFLO17, its encoded protein, and its applications. Through phenotypic analysis of the rice endosperm mealy mutant flo17 and preliminary localization of the target gene, the starch synthesis-related protein OsFLO17 was ultimately cloned. This protein consists of the amino acid sequence shown in SEQ ID NO.3. The starch granule development-related protein of this invention affects the rice endosperm starch synthesis process. Introducing the encoding gene of this protein into plants with abnormal starch granules can yield transgenic plants with normal starch filling. Therefore, the protein and its encoded gene of this invention can be applied to plant genetic improvement.

The invention relates to a method for deep processing of corn by using a pressurized soaking method, in particular to a method for preparing dextrin, syrup or sugar, in which the pressurized soaking method is used for replacing a soaking process of a traditional wet corn processing method, so that the corn quickly absorbs water and swells on the premise of not damaging the quality of germs and endosperm and not discharging waste water and waste gas, and the dextrin, the syrup or the sugar is prepared. The process requirements of a wet corn processing method are met, germs and corn husks are further removed, and the obtained germs and corn husks are refined, so that the germs and the corn husks respectively reach commercial selling standards; directly mixing and liquefying the corn slurry without germs and corn husks; the resulting liquefied liquid may be dried to produce dextrins, or further saccharified, refined, concentrated to produce syrup, or optionally further produce sugars.

The invention discloses a method for accurately identifying sweet-waxy corn germplasm, and particularly relates to a method for identifying sweet-waxy corn germplasm, which comprises a first tracking process: a process from F3-generation seed budding to F4-generation seed maturation, including sweet-waxy gene DNA tracking marking after seed budding; sampling once 28 days after pollination, and measuring the thickness of soluble sugar, amylose, amylopectin and pericarp; extracting DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) of F3-generation plants in a After the F4-generation seeds are mature and picked, determining sweet-waxy gene DNA, determining amylose and amylopectin, and determining single-plant grain weight, hundred-grain weight and endosperm / embryo dry weight; a second tracking process: a process from germination of the F4-generation seeds to bearing of fresh sweet-waxy corn, including observation and recording of a field corn plant growth process; measuring the thickness of soluble sugar, amylopectin, amylose and pericarp 15 days after pollination; determining and analyzing the traits of the indoor test species; data analysis and germplasm scoring: comparing and analyzing the data obtained in the second tracking process, scoring the F4-generation seeds, and determining the germplasm quality of the F4-generation seeds.

The application provides a rice heart-white gene WCG1 and application thereof, and particularly relates to positioning, cloning, function verification and application of a WCG1 gene for controlling a rice kernel heart-white phenotype. A C / A natural variation exists at a ninth exon 986 site of the WCG1 gene, the variation site makes the rice kernel present a heart-white phenotype, that is, a kernel middle heart-white and edge transparent phenotype, and the phenotype is an excellent phenotype of "sake brewing good suitable rice" specially selected in sake brewing.

The present application relates to rice breeding technical field, and relates to a low glycemic index purple rice breeding method. The method comprises the following steps: taking RG4 as the father and taking Zheda Zi 1S as the mother to obtain F1 generation seeds by hybridization; planting the F1 generation seeds, taking the F1 generation as the father and taking Zheda Zi 1S as the mother to obtain BC1F1 generation seeds by backcrossing; planting the backcrossed BC1F1 generation seeds, and obtaining BC1F2 generation seeds by selfing; planting the BC1F2 generation seeds, harvesting normal seed setting fertile excellent single plants, shelling and selecting purple rice, processing into whole white non-waxy rice with endosperm, and then detecting and selecting high resistance starch content strains; planting the BC1F3 generation seeds, selecting and reserving the strains with stable purple rice traits, and determining and selecting strains with whole white endosperm and high resistance starch content; planting the BC1F4 generation seeds, determining and selecting low GI strains; planting the BC1F5 generation seeds, selecting and reserving strains with high alkali solubility value and easy cooking by taking good cooking as the screening index, and breeding low GI purple rice. The rice obtained by the method particularly meets the individual nutritional needs of diabetic patients.

The invention discloses a breeding method and application of fragrant soft rice two-line hybrid rice with an off-white anti-counterfeiting mark, and belongs to the technical field of rice breeding. According to the method, soft rice genes with recessive grey white endosperm markers, genic male sterility character genes and the like are subjected to synchronous phenotype aggregation directional selection for the first time, the problem that the genic male sterility character and excellent taste quality character are difficult to synchronously select in the earliest segregation generation is solved for the first time, and in addition, the technical link of taste quality detection and identification can be saved; the selection efficiency is improved; meanwhile, the problem that the taste quality and control genes thereof need to be detected and identified for multiple times during mating and parent selection of high-quality hybrid rice is solved for the first time; besides, the soft rice character gene with the recessive grey white endosperm marker is synchronously introduced into a two-line hybrid rice parent, through main target character aggregation response identification, the fragrant soft rice two-line hybrid rice with the grey white marker and the aromatic flavor is successfully created for the first time, the yield is increased, and infringement and loss of independent brand fragrant rice products can be prevented.

The invention provides application of a rice endosperm protein content gene, and belongs to the technical field of biology. According to the application, the OsAFB3 gene is used for regulating and controlling the content of the rice endosperm protein. According to the application of the rice endosperm protein content gene provided by the embodiment of the invention, the OsAFB3 gene is used for regulating and controlling the content of the rice endosperm protein, and the effect is remarkable.

The utility model provides a corn degerming post-treatment winnowing device. The corn degerming post-treatment winnowing device comprises an air bellow, an air-closed feeder and an air-closed discharger, the closed air feeder is arranged on the top wall of one end of the air bellow and is used for continuously feeding a mixed material of corn endosperm and corn bud skin into the air bellow; the closed-air discharger is arranged on the bottom wall of the other end of the air bellow; a blanking box is arranged on the bottom wall of the air bellow and right below the closed air feeder; an air inlet channel is arranged on the side wall of one end, close to the blanking box, of the air bellow and is used for blowing air into the air bellow; an air outlet pipe is arranged on the top wall of the air bellow and right above the closed air discharger; wherein a flow guide assembly is arranged in the air bellow, and the flow guide assembly is used for guiding air blown into the air bellow to the air outlet pipe and further used for guiding corn endosperm and corn bud husks which are separated according to the gravity difference under the action of wind power to the blanking box and the closed air discharger respectively. According to the corn degerming post-treatment winnowing device, the endosperm winnowing extraction efficiency and purity after corn degerming can be improved.

A method for obtaining highly sulfated nanoparticles of cellulose or sulfated nanoparticles of mannan, or a combination thereof, from endosperms, comprising acquiring a ground flour of an endosperm of a seed, nut, or pit, wherein the ground flour contains polysaccharides, and wherein the polysaccharides contain either cellulose or mannan, or both cellulose and mannan; pretreating the ground flour to condition the polysaccharides for hydrolysis and functionalization thereof; preparing a reagent for hydrolysis and functionalization of the polysaccharides, wherein the reagent comprises a solution of glycerol, water, and sulfuric acid at predetermined concentrations; adding the reagent to the pretreated polysaccharides to form a suspension, wherein adding the reagent to the suspension converts the cellulose or mannan or both into nanoparticles that are sulfated by the reagent; separating the sulfated nanoparticles from the suspension; washing the nanoparticles; and creating a stable colloidal suspension of the sulfated nanoparticles. The sulfated nanoparticles are used in pharmaceutical, cosmetic, wound healing, and skin treatment formulations.

The invention relates to low-damage endosperm rice and a preparation process thereof. Specifically, the invention provides a brown rice milling method, the method comprises the steps of unhusked rice opening and fine milling, and the method comprises the step of controlling the protein retention rate, the damaged starch content and the protein-to-ash ratio of milled brown rice to be in the following ranges: the protein retention rate is 88.0% or above, the damaged starch content is 0.480% or below, and the ash-to-protein ratio is 0.055 or above.

The present invention discloses a plant gluten sorting-related protein OsGPA15, its encoding gene and applications. Through phenotypic analysis of rice gluten sorting mutants gpa15 and preliminary mapping of target genes, the gluten sorting-related protein OsGPA15 was finally cloned. The related protein is composed of the amino acid sequence shown in SEQ ID NO.1, and the nucleotide sequence of the gene is as shown in SEQ ID NO.2 or SEQ ID NO.3. The gluten sorting-related protein of the present invention affects the sorting process of gluten in rice endosperm. By introducing the encoding gene of the protein into plants with reduced mature gluten content, transgenic plants with normal mature gluten content can be obtained. Therefore, the protein and its encoding gene of the present invention can be applied to plant genetic improvement.

The invention belongs to the field of plant engineering technology and crop germplasm resource innovation, and particularly relates to a rice BEIIb gene mutant, application of the rice BEIIb gene mutant in improving rice resistant starch and an improving method. A mutant material with high resistant starch content is created by knocking out the rice BEIIb gene, and compared with a wild type, mutant seeds show an obvious chalky phenotype, composite starch particles are abnormal in a spherical or ellipsoidal shape, the amylose content is remarkably increased, the protein content is remarkably reduced, the resistant starch content is remarkably increased, and the resistant starch content is remarkably reduced. The hardness of cooked rice is remarkably increased, and the elasticity is remarkably reduced. Through transcriptomics analysis, the protein mainly participates in biological pathways such as carbohydrate metabolism, chloroplast photosynthesis, calmodulin combination and secondary metabolite synthesis, and the expression level of endosperm starch synthesis related genes is obviously changed. The method can provide theoretical basis and method reference for breeding of high-quality rice varieties with high resistant starch.

The invention relates to application of a rice endosperm specific expression gene NF-YA8 in rice quality heat resistance at natural high temperature in a field. The NF-YA8 gene is separated from rice, and the function of the NF-YA8 gene in negative regulation and control of rice quality heat resistance is successfully identified through a transgenic material, namely negative regulation and control of rice quality (chalky appearance quality, storage substance content and the like) heat resistance at natural high temperature of a field; the invention provides a new gene resource for rice quality breeding under the global warming background, has very important significance for cultivating high-temperature-resistant rice varieties, provides a new gene resource for high-quality breeding of rice, and also provides a technical reference for cloning related genes in other crops.

The invention discloses an efficient corn germ extracting machine which comprises a bottom plate, two mounting rods are fixedly mounted at the top of the bottom plate, a same fixing plate is fixedly mounted at the tops of the two mounting rods, a hydraulic cylinder is fixedly mounted at the bottom of the fixing plate, and a follower plate is arranged below the hydraulic cylinder; and a filter basin is arranged below the follower plate. The hydraulic cylinder drives the driving plate and the follow-up plate to synchronously move downwards, so that the follow-up plate is tightly attached to the top end of the filter basin to achieve sealing of the top end, dust overflowing and material splashing are effectively avoided, then the hydraulic cylinder continues to push the driving plate to move downwards to start the servo motor, the rolling block rotates at a high speed while swinging and rolling, and the dual treatment effect is achieved; the separation efficiency and purity of corn germs and endosperm are greatly improved, in the treatment process, dust and chippings in the filter basin can be sucked and collected, dust harm is reduced, finally, a hydraulic cylinder reversely drives a driving plate to move upwards, back flushing cleaning is conducted on the inner wall of the filter basin, and residual material accumulation is avoided.

The invention belongs to the field of gene engineering, and relates to a chalkiness-related protein OsGAPDH of rice endosperm as well as a coding gene and application of the chalkiness-related protein OsGAPDH. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO: 3 in a sequence table; and (b) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence of SEQ ID NO: 3, is related to plant starch synthesis and is derived from SEQ ID NO: 3. The protein provided by the invention affects normal development of rice endosperm. The coding gene of the protein is introduced into Ningjing No.7 wild type, so that a transgenic plant of which the chalkiness of endosperm is obviously reduced can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The present invention discloses a method for sterile germination and seedling raising of the rhizome of polygonatum sibiricum redoute, a medicinal plant from the Maoshan area. The specific steps are as follows: collect immature fruits of polygonatum sibiricum redoute, soak the fruits with a medicament first to make the pulp of the berries rot, rub and wash out the seeds of polygonatum sibiricum redoute, then put them into a mixed solution of kinetin and gibberellin and mix them with river sand for low-temperature sand storage. The seeds obtained after the sand storage treatment are sterilized and disinfected. After cutting off part of the pericarp and endosperm to expose the embryo, they are put into a cluster bud induction medium for liquid oscillation culture to induce cluster buds. Finally, the cluster buds are transferred to a strong seedling and rooting medium for strong seedling and rooting culture to form tissue culture seedlings of polygonatum sibiricum redoute. The method provided by the present invention uses immature embryos of polygonatum sibiricum redoute for medicament treatment, avoiding the dormancy stage after the seeds of polygonatum sibiricum redoute mature, and increasing the seed germination rate to more than 90%. And through the two-step method of liquid oscillation culture and rooting culture of the embryos of polygonatum sibiricum redoute, the cycle of rapid propagation and seedling raising of the seeds of polygonatum sibiricum redoute is greatly shortened.

The invention discloses an HRM primer and application of the HRM primer to identification of plant-derived components of tara and gleditsia. Aiming at the defect of lack of a product and a method for identifying high-value gleditsia sinensis lam pretended by low-cost tara seed endosperm at present, nuclear gene DNA of tara, gleditsia sinensis lam for producing gleditsia sinensis lam and common congeneric related species thereof are analyzed; an HRM primer capable of distinguishing plant-derived components of tara and gleditsia is obtained based on ITS sequence design. On the basis of the HRM primer, the invention also provides a kit and a method for identifying the tara and the gleditsia plant-derived components, and the tara can be rapidly and specifically detected, so that the identification of the gleditsia sinensis seeds adulterated with the tara seed endosperm is realized. The method is beneficial to inspection of adulterated gleditsia sinensis fruits, and economic benefits of consumers and gleditsia sinensis fruit commercial tenants and enterprises are protected.

[Problem] To provide a composition for regulating blood pressure for a healthy person. [Solution] A blood-pressure-regulating composition for a healthy person, said composition being characterized by containing a guar gum degradation product, and being characterized in that: 70 mass% or more of the guar gum degradation product has an average molecular weight within the average molecular weight range of 1.0 × 103 to 1.0 × 105; the composition has a viscosity of 10 mPa·s or less when a 2 mass% aqueous solution at 37°C is measured at 10 rpm using an E-type viscometer, or has a viscosity of 5 mPa·s or less when the aqueous solution is measured at 20 rpm, or has a viscosity of 3 mPa·s or less when the aqueous solution is measured at 30 rpm; the composition is obtained by hydrolyzing a galactomannan polysaccharide which contains guar-derived endosperm and in which the content ratio (galactose:mannose) of galactose and mannose is within the range of 1:1.3 to 1:2, by using a microorganism-derived β-mannanase to reduce the molecular weight of the polysaccharide; and the composition contains at least 70 mass% of dietary fiber in terms of the dietary fiber content stipulated in the enzyme-HPLC method.

The invention discloses a novel coronavirus variant RBD protein gene and application thereof. The method comprises the following steps: firstly, respectively constructing recombinant plant expression vectors pGt1-RBD and pGt1-RBDopt containing RBD and RBDopt genes, carrying out agrobacterium-mediated transformation to obtain a T0-generation transgenic rice plant, and carrying out T0-T3-generation screening and identification to obtain a transgenic rice plant capable of stably expressing RBD protein. The RBD protein is successfully expressed in rice endosperm and is subjected to N-glycosylation modification, and the content of the RBD protein in pGt1: RBDopt is obviously higher than that of pGt1: RBD. The pGt1:: RBDopt transgenic rice flour suspension is orally taken into an intragastric mouse, and experimental results show that the transgenic rice pGt1:: RBDopt can be used for developing oral vaccines of new crown vaccines and has a good application prospect.

The invention discloses an application of an oil body protein gene OsOle4 in cultivation of rice strains with different fatty acid contents, the gene specifically regulates and controls the lipid content and cooking taste quality of rice, and does not influence the activity of rice seeds and the growth and development of rice. The OsOle4 is subjected to gene editing and endosperm specific overexpression by adopting a conventional method, so that new rice germplasm with different OsOle4 gene expression levels is obtained. Compared with parent control, the rice germplasm created by the invention has the advantages that the germination rate of seeds, growth and development of plants and basic agronomic traits are not obviously changed, but the rice lipid content of OsOle4 mutant plants is obviously reduced, and the rice lipid content of overexpression plants is obviously increased. Besides, OsOle4 mutation and overexpression plants both show the tendency of cooking taste quality increase, useful gene resources are provided for genetic improvement of rice quality traits, and important breeding utilization value is achieved.

The wheat flour impacting and loosening machine comprises a first impacting bin and a second impacting bin, the first impacting bin is arranged at the corresponding position above the second impacting bin in the vertical direction, the first impacting bin and the second impacting bin are fixedly connected through an inverted-cone-shaped conical pipe, and a pipe opening in the lower end of the conical pipe communicates with a feeding pipe in the center of the top face of the second impacting bin; the conical pipe is sleeved with a spiral pipe, a discharging opening in the side end of the first impact bin is communicated with a pipe opening in the upper end of the spiral pipe, and a pipe opening in the lower end of the spiral pipe is tangentially inserted into the side wall of the conical pipe. According to the impact flour loosening machine for the wheat flour, the wheat flour materials are subjected to impact flour loosening twice through the first impact bin and the second impact bin, flour pieces of the wheat flour can be further crushed, the separation effect of endosperm and bran is enhanced, and the flour yield is increased.

Methods for producing a purified wheat embryo product are disclosed. In one embodiment, producing a purified wheat embryo product includes the steps of: accelerating a plurality of wheat berries toward an impact surface, impacting each of the plurality of wheat berries against the impact surface, dislodging at least some of the wheat embryos from the wheat berries in response to the impacting step such that the dislodged embryos are intact, and separating the dislodged wheat embryos from the bran and the endosperm to produce an intermediate purified wheat embryo product.