77 results about "Paraffin embedding" patented technology

The invention discloses a manufacturing method of paraffin sections of zostera marina embryo, comprising the following steps: (1) material drawing and fixation: zostera marina seeds are taken and the fruit skin is stripped, and after endosperm is removed, an integral zostera marina embryo is soaked in FAA (free amino acids) fixing solution and is fixed for more than 48 hours; (2) dehydration: the zostera marina embryo is taken out from the fixing solution and is soaked in alcohol for dehydration; (3) waxing and embedding: paraffin is added gradually in a vessel containing the zostera marina embryo and dimethylbenzene, and conventional paraffin embedding is carried out to obtain the wax blocks containing the zostera marina embryo; (4) slicing, spreading and drying: the wax blocks coated with the zostera marina embryo is fixed on a wheel rotation type slicing machine for carrying out continuous slicing of the paraffin, so as to obtain wax bands and enable the wax bands to be attached to an object slide; (5) dewaxing, rehydration and dyeing of hematoxylin dye solution; (6) slicing, dehydration and dyeing of eosin and alcohol; and (7) transparence and mounting are carried out to manufacture a permanent section. In the manufacturing method disclosed by the invention, the paraffin sections of the zostera marina embryo, which have clear dyeing and an integral texture structure, can be obtained.

The invention discloses a production method for a paraffin section of a paeonia lactiflora mature embryo. The production method comprises the following steps: drawing materials and fixing: stripping a seed coat, cutting a seed, immersing the seed top part containing the embryo or part endosperm into a FAA stationary liquid, and fixing for more than 24 hours; dehydrating: washing in ethyl alcohol with the concentration of 70% for 3 to 5 times, and performing gradient dehydration from the ethyl alcohol with the concentration of 70%; performing hyalinization: carrying out five level hyalinization by using dimethyl benzene as a transparent reagent; waxing and embedding: pouring out one half volume of dimethyl benzene, adding wax crumbs in a bottle until saturation, staying overnight at the temperature of 40 DEG C; then changing a wax liquid every two hours for three times per day, repeating for three days, and carrying out conventional paraffin embedding; slicing; flattening and bonding: flattening a linoleum tape on an iron plate of a water bath kettle at the constant temperature of 50 DEG C, dewaxing, rehydrating, dyeing through safranine and fast green and performing hyalinization; mounting. According to the invention, the paeonia lactiflora mature embryo paraffin section of which the tissue structure is integrated and clear can be obtained, so that accurate technical support can be provided for researching paeonia lactiflora seed dormancy and germination.

A process for the production of paraffin sections of biological tissue, especially for molecular pathology studies is disclosed. In the process, the tissue sample is simultaneously fixed, dehydrated and cleared in a first step, subsequently dehydrated and cleared in a second step and infiltrated with an inert specimen matrix in a third step. The specimen can then be further embedded in a casting supporting matrix according to the standard procedures followed by any local pathology or research laboratory. A kit and a processing station for automating paraffin embedding of a tissue sample suitable for histopathological and molecular analysis is also described. A bio-indicator system is described for measuring the degree of crosslinking. A tissue sample holding means or a vial which includes a tissue sample holding means provided with a data logging device capable of registering and transmitting data regarding the sample and conditions where the sample was processed is also disclosed.

Disclosed is an environmental protective automatic continuous paraffin-embedding device which includes a shell body and a workbench, wherein the shell body and the workbench are fixedly connected to each other. The workbench is provided with three guide rails, wherein two guide rails are fixed on the workbench in parallel through two supports and two ends of the third guide rail are respectively arranged on the two guide rails. A first installation groove is formed in the workbench. A paraffin-accommodating box and a cooling box are arranged in the first installation groove. A heating apparatus is disposed on the exterior of the bottom of the paraffin- accommodating box and a filter screen is arranged in the paraffin-accommodating box. The cooling box refrigerates through a refrigerating apparatus. The first installation groove is provided with an embedding plate which is provided with an embedding mould and an embedding box. A paraffin box is arranged in the shell body. A stop valve and a paraffin pipe are disposed on the bottom of the paraffin box. An outlet of the paraffin pipe is arranged on a slide block of the third guide rail, is adjusted through a telescoping apparatus and sucks residual paraffin up through a paraffin-refluxing pipe. An unfolding bench and an organization box are arranged on the filter screen in the paraffin-containing box. The paraffin-embedding device can be used for manufacturing a plurality of paraffin embedding block, has complete functions, is safe and environment-friendly and is safe to use.

The invention relates to the field of plants, and especially relates to a paraffin sectioning method of a plant tissue. The method is characterized in that buckwheat husk is immersed in warm water to make the water content of the buckwheat husk being 20-40%, a water-containing tissue paraffin embedding technology is adopted to substitute dehydration, transparentization and paraffin infiltration steps of routine paraffin sectioning, and a dissecting needle is used to strip paraffin instead of paraffin removal in the section flatting process, so the time and the operation of the section process are greatly reduced. Buckwheat husk tissue sections with the thickness of 10-16[mu]m can be obtained through the method, and the method makes the tissues complete and display of all positions clear, and obviously enhances the sectioning and observation effects.

The invention provides a TEM sample preparation method. Based on the paraffin embedding technique, a sample is effectively prevented from being cracked or chipped during the mechanical milling and thinning process, and then the sample is directly and quickly ground to be 20-30 mum in size. In this way, the condition that a sample easily cracks or breaks down when being continuously ground to be smaller than 50 mum in size after being bonded to a copper ring according to the conventional method can be avoided. The sample preparation success rate is significantly increased, and the sample preparation period is significantly shortened. Based on the paraffin dissolving and diluting technique, the adhesive force of paraffin for the sample is lowered. Therefore, the sample can be successfully and completely transferred. According to the technical scheme of the method, the sample preparation success rate is increased from 60-70% to 90-100%. Meanwhile, the sample preparation period is shortened to be 100-180 minutes.

The invention discloses a paraffin texture chip preparation method which is a rapid, simple, economic and applicable preparation method, particularly aiming at the preparation of a texture microchip of which the diameter is less than 0.5mm; and the preparation method is precise and reliable. The preparation method specifically comprises the steps of punching holes and positioning, using a needle wire attached gel arranging isolating method, carrying out paraffin embedding, cooling, taking down the needle by using a release agent, and performing refusion so as to obtain the texture microchip. The paraffin texture chip prepared by using the preparation method has the advantages of being tidy in arrangement, uniform in interval, and high in chip preparation rate after being sliced, economic, practical, and low in cost, and cells are clear and visible without damage after being dyed, so that the texture microchip technology can be popularized and used more widely.

The invention relates to a glioma typing system based on IDH1-R132H and ATRX expression. The system comprises: (1) glioma patients' tumor paraffin embedding kit and optional kit application instruction; (2) a kit for immunohistochemical detection of ATRX protein expression level of tumor of glioma patients and optional kit application instruction; and (3) a kit for immunohistochemical detection of IDH1-R132H protein expression in tumor of glioma patients and optional kit application instruction. When IDH1-R132H and ATRX losses are combined as diagnostic markers for distinguishing primary glioblastoma, WHO grading II/III oligodendroglioma, WHO grading II/III astrocytoma and secondary glioblastoma, diagnostic specificity is obviously higher than diagnostic specificity of the two single markers of IDH1-R132H or ATRX losses.

The invention discloses a kit for detecting expression condition of a paraffin embedding operative specimen of an esophagus cancer patient and further predicting prognosis of the patient by combining 5-lipoxygenase metabolic pathway protein antibodies. The kit comprises three target protein antibodies: 5-LO, FLAP and LTA4H antibodies, and also comprises goat serum, 0.01M citrate repair liquid, 3% H2O2, a Polymer enhancer, a Polymer polymer, a DAB chromogenic reagent and PBS solution. Compared with single index detection, the kit for predicting the prognosis of the patient by combining 5-lipoxygenase metabolic pathway proteins has higher prediction effect, ranking only second to prediction ability of TNM staging. Moreover, the immunohistochemical ultrasensitive two-step method in the invention is a mature and reliable method which can be widely used in primary hospitals.

The invention relates to automatic thermostatic electric heating tweezers, which consist of a thermostatic tweezers body, an operating tweezers handle, a PTC heating device, a heating power supply, a display circuit, a box, a quick joint, a quick joint seat and an insulating lead. The automatic thermostatic electric heating tweezers are characterized in that: the thermostatic tweezers body and the operating tweezers handle are connected to form a whole, the PTC heating device is fixed on the middle part of the tweezers, and the PTC heating device is connected with the heating power supply through a cable and the quick joint. The heating power supply, the display circuit and the quick joint seat are assembled in the box together. The electric heating tweezers synthesize the advantages of the novel PTC heating device, the electronic technology and the structure design of the thermostatic tweezers body and the operating tweezers handle. A constant temperature of about 60 DEG C is provided at the tweezers body part. Therefore, pathologic tissue components are not adhered on the tweezers, and the clinical diagnosis error is reduced. The operating tweezers handle has no hand burning feeling. The tweezers eliminate cross infection among the pathologic tissues, provide obvious reliable effect for qualification of a specimen, are simple and convenient to operate, have the advantages of reducing the working steps and improving the working efficiency, and are particularly suitable to be used in pathologic tissue paraffin embedding.

The invention provides a bone marrow specimen processing method, a decalcification solution, and applications. The bone marrow specimen processing method comprises following processing steps of bone marrow tissues: fixing and primary decalcification, dehydration, paraffin embedding, rough trimming, secondary decalcification, and slicing; the decalcification solution at least comprises formic acid,formaldehyde, and distilled water at a volume ratio of 198-202:48-52:190-210, wherein the mass concentration of formic acid ranges from 97 to 99%. The bone marrow specimen processing method is used for processing bone marrow specimen, so that slices with relatively complete tissue structures and clear cell structures can be obtained, and it is beneficial for observation of slices under microscopes.

The invention belongs to the field of biotechnology and particularly relates to a micron-sized biological material paraffin sectioning method. The method comprises the following steps of fixing a micron-sized biological material, i.e. suspending the micron-sized biological material in serum to obtain a mixture of the micron-sized biological material and serum; slowly filling the mixture of the micron-sized biological material and serum into Bouin fixing liquid to obtain a precipitate wrapping the micron-sized biological material; dehydrating the obtained precipitate to obtain a pre-processed material; performing conventional paraffin embedding and sectioning on the obtained pre-processed material. According to the micron-sized biological material paraffin sectioning method provided by the invention, the micron-sized biological material can be simply and effectively fixed, the defects that deformation is easily caused, sectioning is not continuous, or existence of cavities causes incomplete sectioning and the phenomenon that sectioning cannot be performed when the micron-sized biological material is sectioned are overcome, the sectioning thickness can be up to 5 microns. The method is simple in operation, special equipment is not required, a reagent is easy to obtain, and the method is convenient to popularize and use.

The invention relates to an IDH1-R132H and ATRX expression based glioma prognostic system which comprises (1), a tumor paraffin-embedding kit for glioma patients and an operating instruction thereof if necessary; (2), a kit for immunohistochemically testing ATRX protein expression level of tumor of glioma patients and an operating instruction thereof if necessary; and (3), a kit for immunohistochemically testing IDH1-R132H protein expression of tumor of glioma patients and an operating instruction thereof if necessary. Progression free survival (PFS) of WHO II-level and III-level of glioma is typed according to loss states of IDH1-R132H and ATRX; patients with both loss of IDH1-R132H and ATRX has longer PFS than patients with both expression of IDH1-R132H and ATRX, and PFS of patients with IDH-WT is close to PFS of patients with glioblastoma.

The invention discloses a method for observing a microtubule structure of a cytoskeleton in a liver tissue of animals. The method comprises the following steps: (1) cleaning the liver tissue of animals with PBS, fixing for 8-12h with 4v/v% of paraformaldehyde water solution, and carrying out paraffin embedding and serial sectioning; (2) dewaxing the obtained paraffin sections, and carrying out antigen retrieval, closing and fluorescently labeled antibody incubation; (3) for specimens sealed by glycerol, observing through a laser scanning confocal microscope within 24h, respectively exciting 488 and 4' 6-diamidino-2-phenylindole under 488nm and 405nm, and observing and shooting at once at room temperature in a dark room environment. The method is fast, simple, intuitive and accurate, is strong in practicality and extensibility, and can research the dose-effect changing relationship of the cytoskeleton under stress of pollutants.

The invention belongs to the technical field of plant sectioning and discloses a beet leaf paraffin-embedded sectioning method, comprising: taking and immobilizing a material, to be specific, cuttingthe material, adding in a wide-mouth bottle, and immobilizing for 24 h; dewatering and transparentizing, to be specific, dewatering for 2 h, and transparentizing for 1.5 h; staining with safranin andfast green; refining with paraffin, to be specific, performing paraffin immersion with a 75 DEG C thermostatic box; using a semiautomatic embedding machine to perform embedding, placing the material in an embedding box, setting the material in position, and placing the embedding box on ice cubes made with an ice breaker for quick cooling; trimming, sectioning, sticking sections, and removing paraffin; sealing the sections, to be specific, sealing the sections in wet form, and drying by natural air. The inner tissue structure of beet leaf is made clear herein; the key steps, such as material taking and immobilizing, dewatering and transparentizing, paraffin melting, paraffin immersion, paraffin embedding, sectioning, section sticking, staining, and section making, are optimized; time is saved, slice making efficiency is improved, and success rate is increased.

The invention relates to a paraffin embedding device for tissue positioning and embedding. The paraffin embedding device comprises an embedding mold and a glue sealing base, wherein the glue sealing base comprises a bottom plate and side plates arranged on the two sides of the bottom plate; a groove penetrating through the bottom plate in the width direction is formed in the part, at one end of the sealing glue base, of the bottom plate; blades with cutting edges exposed out of the bottom plate are fixedly mounted on the inner walls of the two ends of the groove; the side plates at the two ends of the groove are provided with rotating shafts which are transversely arranged on the glue sealing base; elastic pressing sheets are further arranged on the side plate on one side of the upper parts of the rotating shafts; fixing seats are arranged on two side plates at the other end of the sealing glue base; the fixing seats on the two side plates are provided with through holes, supporting shafts transversely arranged on the glue sealing base penetrate through the through holes, the supporting shafts are provided with PET transparent adhesive tapes, the embedding mold is a box body with the top and the bottom hollowed out, the length of the embedding mold is smaller than that of the groove by 5-20 mm, and the width of the embedding mold is equal to that of the bottom plate. The problem that a knee joint cannot be embedded correctly due to the fact that paraffin is solidified too fast in the embedding process of a traditional mold is solved.

The invention discloses a method for measuring space structure parameters through a scanning image of a forest litter and belongs to the technical field of forest ecology. The method comprises the following steps: performing paraffin embedding, slicing and scanning to obtain a forest litter longitudinal section image; calculating the width and height sizes of the image according to the dots per inch (DPI) resolution of the scanning image; constructing a measurement coordinate system in millimeters in Arcgis9.3 by taking four corners of the image as coordinate points, and eliminating invalid parts of image data by determining a valid image data area; extracting litter and pore information by using Erdas Imagine9.2 texture analysis and ISODATA classification tools, and converting the litter and porosity information into a vector graph layer; constructing a layered grid by using an Arcgis9.3 Create Fishnet tool to partition a pore graph layer, performing layered statistics to obtain porosity, and calculating the distribution direction of each layer of porosity graph spots by using a Polar Plots and Circular Statistics module. A technical support is provided for further research on decomposition of the litter, matter circulation, soil microorganisms, water conservation, preservation of water and soil characteristics, seed germination and the like.

The invention discloses a method for observing a same sample by using a paraffin section and a scanning electron microscope. The method comprises the following steps: subjecting the sample to paraffin embedding and then carrying out tissue sectioning until the symmetric axis of the sample is reached, or according to observation of tissue sectioning results; then subjecting the rest embedded paraffin block of the sample to deparaffinization; and then carrying out scanning via the electron microscope so as to obtain electron microscope scanning results of a sample section. According to the invention, one same sample is made full use; the morphologies of a variety of cells in the sample can be observed, and the outer contour of tissue and high-magnification high-resolution three-dimensional images of tiny structures in the tissue can be observed in the obtained electron microscope scanning results of the sample at the same time, which are favorable for research on the morphological development of plants.

The invention relates to the biological field, particularly relates to a staining method of swimming crab muscle spore worms and specifically relates to a staining method aiming at parasitic swimming crab muscle spore worms in muscles of portunus trituberculatus. The staining method comprises the following steps: fixing a swimming crab muscle tissue by virtue of a Davidson's AFA fixation liquid; producing a tissue paraffin embedding block; carrying out xylene dewaxing; carrying out ethanol gradient hydration; staining by virtue of a Weigert iron hematoxylin stain solution; staining by virtue of a ponceaux acid product red solution; carrying out restaining by virtue of an aniline blue solution; and carrying out ethanol dehydration, carrying out transparentization by virtue of xylene, and airing, so as to obtain a neutral vegetable glue seal piece. By staining pathological muscle tissues by virtue of the staining method provided by the invention, muscle fibers are of a blue strip shape, and spores of the swimming crab muscle spore worms are of a red oval shape, have clear morphological structures; and compared with background tissues, the spores of the swimming crab muscle spore worms are clear and easy to distinguish.