14 results about "Paraffin embedding" patented technology

This invention provides a method for preparing pathological sections of plague-affected experimental animals. The method includes the following steps: S1: sample pretreatment and fixation; S2: pathogen inactivation verification; S3: tissue dehydration, clearing, and paraffin embedding; S4: paraffin embedding; S5: sectioning and spreading; S6: staining; S7: mounting. The method for preparing pathological sections of plague-affected experimental animals provided by this invention has the advantages of ensuring absolute safety in the preparation process of high-risk pathogen sections through strict biosafety zoning and double inactivation verification, and obtaining high-quality pathological sections.

This invention relates to the field of paraffin sectioning technology and discloses a paraffin section pretreatment method suitable for plant spatial transcriptomics, comprising: (1) sample collection and fixation; (2) sample dehydration; (3) clearing; (4) paraffin embedding; (5) paraffin section preparation; and (6) pretreatment of sections before RNA FISH. This invention can be widely applied to various plant tissues, and its detection sensitivity can reach the visualization of low-expression genes (FPKM≤20), contributing to the popularization and development of plant spatial transcriptomics.

The application discloses pathological tissue paraffin embedding device, relates to the technical field of medical devices, and relates to embedding forceps, a pressing block and a temperature controller, wherein the embedding forceps comprise a forceps handle and two forceps arms; the two forceps arms are in heat conduction connection with the forceps handle; the pressing block comprises a flattening end face, the flattening end face is used for flattening pathological tissue, one side of the pressing block, which is away from the flattening end face, is detachably connected with an end of the forceps handle, which is away from the forceps arms, and the pressing block and the forceps handle are in heat conduction contact in the connected state; and the temperature controller is in heat conduction connection with the forceps handle through a heat conduction wire. Wherein the flattening end face is provided with a first anti-sticking layer with micro-texture, and the pressing block is provided with exhaust grooves on the peripheral side. In the application, the pressing block is detachably connected with the embedding forceps, the embedding forceps can be rotated by 180 degrees to be operated by using the pressing block, the operation is simple and convenient, and the working efficiency can be improved; and the embedding forceps and the temperature controller are designed to be separated, are connected through the heat conduction wire, the embedding forceps are simple in structure, long in service life and low in maintenance cost.

The invention discloses a paraffin embedding demolding device for an umbilical cord tissue sample, and relates to the technical field of sample embedding, and the paraffin embedding demolding device comprises a base, a mounting piece, a bottom mold arranged on the mounting piece and an embedding box arranged on the bottom mold; a locking structure corresponding to the bottom die is arranged on the mounting piece; a cavity is formed in the bottom die, external rectangular columns are symmetrically arranged on the two long outer walls of the bottom die, circular channels communicated with the cavity are formed in the external rectangular columns, pistons are arranged in the circular channels, the upper ends of piston rods of the pistons extend out of the external rectangular columns, and supporting rods are fixedly arranged at the extending ends; external connecting plates are fixedly arranged on the outer edges of the upper parts of the two long outer walls of the embedding box, and when the embedding box is placed on the bottom die, the external connecting plates are lapped on the supporting rods; a hard air pipe capable of being communicated with the cavity is fixedly arranged on the base, and a sealing air channel communicated with the hard air pipe is arranged on the base. The paraffin embedding and demolding device for the umbilical cord tissue sample has the beneficial effect that the paraffin embedding and demolding device for the umbilical cord tissue sample is provided.

The invention relates to the field of paraffin embedding, and discloses a paraffin embedding device for pathological sections and a control method thereof, and the paraffin embedding device comprises a workbench, a paraffin filling device, a transportation component, a freezing table, temperature control equipment and an embedding mold. The workbench is provided with a wax cavity, a moving groove and a heat dissipation cavity. The wax filling device comprises a first wax dripping machine and a second wax dripping machine; the freezing table comprises a first freezing plate and a second freezing plate; the conveying component comprises a roller and a roadblock assembly which are arranged; the temperature control equipment adopts a refrigerating system composed of a compressor, a condensing coil, a throttling mechanism and an evaporating coil, part of the condensing coil is arranged in the wax cavity to provide heat, part of the condensing coil is arranged in the heat dissipation cavity to dissipate heat, and the evaporating coil refrigerates the freezing table. Through staged embedding and freezing processes and in combination with automatic transportation, the embedding quality and efficiency are effectively improved; meanwhile, the paraffin is heated by waste heat of a refrigerating system, so that energy recycling is realized, and the method has the advantages of energy conservation, environmental protection, simplicity and convenience in operation, stable embedding quality and the like.

The invention discloses a cell preservation method and application, and the cell preservation method comprises the following steps: obtaining a target cell, and fixing the target cell; wrapping the fixed target cells with gel; the gel contains carbomer, a protease inhibitor, a cell permeation agent, a preservative and a buffer component, and directly preserving the target cells wrapped by the gel or embedding the target cells in paraffin to obtain a cell paraffin block for preservation. The cells fixed by the method can be directly preserved for a long time at 4 DEG C without dehydration, transparency and embedding, the gel concentration is adjusted to be liquefied before use, and the fixed gel can be removed by centrifugation; or paraffin embedding can be carried out according to a general process after gel wrapping, and paraffin is sliced before use. Therefore, the cell protein can be stored for a long time without complete denaturation, and meanwhile, an antigen repair process is not needed during use, so that the time is saved, and the efficiency is improved.

The invention provides a FISH (fluorescence in situ hybridization) probe for detecting a new coronavirus Armike strain as well as preparation and application of the FISH probe. The FISH probe comprises three steps of determining a CDC-O target area, designing and synthesizing an oligonucleotide library, amplifying a probe template library and preparing the fluorescent probe. The prepared FISH probe is uniform and controllable in size, the size ranges from 20 bp to 100 bp, and the FISH probe can easily penetrate through a cell nuclear membrane, so that the hybridization efficiency is improved; meanwhile, the preparation method is not limited by BAC cloning, and only one-time chemical synthesis is needed, so that the synthesis cost is reduced, and the batch difference caused by repeated synthesis can be reduced. The probe can be used for in-vitro qualitative detection of the Obemitra virulent strain in a new coronavirus patient tissue slice soaked by formalin or embedded by fixed paraffin, so as to achieve the purpose of accurate diagnosis.

The utility model discloses a paraffin embedding device for an experimental sample, and relates to the technical field of medical instruments, in particular to a base, a main body is fixedly mounted at the upper end of the base, a peristaltic cavity is formed in the inner side of the main body, a clamping ring is fixedly mounted on the inner side of the peristaltic cavity, a peristaltic tube is arranged on the inner side of the clamping ring, and the peristaltic tube is arranged on the inner side of the main body. One side of the bottom end of the base is fixedly connected with a liquid discharge pipe, and one end of the peristaltic pipe penetrates through the main body and is fixedly connected with the liquid discharge pipe. The heater is matched with the metal ring so that the metal ring can be heated, the material box can be heated through the metal ring so that paraffin on the inner side of the material box can be melted, the rotary disc is used for driving the multiple extrusion wheels to rotate and be matched with the peristaltic pipe so that suction can be achieved, and paraffin solution can be discharged outwards in cooperation with the liquid discharging pipe; a part of heat of the metal rings can be transferred through the heat conduction strips, so that blockage caused by the fact that paraffin is easily solidified on the inner side of the peristaltic pipe can be avoided.

The invention provides a cell quality control product and a preparation method thereof, and belongs to the technical field of immunodetection quality control products. According to the application, the cell quality control product is obtained by fixing the cell aggregate by using the homologous bifunctional cross-linking agent and carrying out permeation treatment. When the cell quality control product is prepared by using the preparation method disclosed by the invention, the steps of paraffin embedding, slicing and the like are not needed, so that time and reagents can be saved, the integrity of cells can be maintained, the loss of the cells is reduced, and the yield is improved. Moreover, no matter the target spot is membrane protein or plasma protein or nucleoprotein, the target spot can be well fixed, and the dyeing performance of the target spot is good.

The utility model relates to the technical field of paraffin embedding, in particular to tweezers which comprise a first tweezers piece, a second tweezers piece is welded to one side of the first tweezers piece, through holes are formed in the two sides of one end of the first tweezers piece, rotating rods are rotationally connected into the through holes, and limiting rings are installed at the two ends of the surface of each rotating rod. The two ends, close to the middle, of the surface of the rotating rod are sleeved with return springs, the two ends, close to the limiting ring, of the surface of the rotating rod are rotationally connected with forceps heads, and a clamping groove is formed in the middle of one side of each forceps head. The improved tweezers can provide more stable and uniform pressure when detritus tissues are treated, are effectively suitable for tissue samples which are small in size, thin and large in number, rapidly and accurately compact all the tissues, reduce the risks of tissue loss and mutual pollution, effectively compact the tissues and improve the working efficiency. And moreover, the device can adapt to embedding boxes of different models, so that the flexibility and the efficiency of operation are improved.

This invention discloses a method and application for detecting goblet cell-related antigen channels in the piglet intestine. It relates to the field of antigen detection technology. The method includes the following steps: taking a portion of the mid-jejunum from a piglet; processing it; cutting the processed mid-jejunum into small pieces; incubating the pieces in a culture medium containing a marker; fixing; dehydrating and clearing the fixed pieces; embedding them in paraffin; preparing tissue sections; staining the tissue sections with a rabbit antibody against the goblet cell marker protein mucin 2 and a goat anti-rabbit fluorescent secondary antibody; mounting with the nuclear dye DAPI; and observing and analyzing under a fluorescence microscope. The method provided by this invention is simple to operate and can successfully detect goblet cell-related antigen channels in weaned piglets, laying the foundation for subsequent pig nutrition formulations.

The invention discloses an organoid rapid paraffin embedding method, and relates to the technical field of organoid embedding. The method comprises the following steps: inoculating an organoid into matrigel, uniformly mixing, and adding a culture solution for culturing; then absorbing and removing the culture solution, cleaning, and fixing the organoid by using a fixing solution; removing the stationary liquid, cleaning, adding gel, and cooling at room temperature until the gel is solidified into gel blocks; and finally, dehydrating the rubber block, and embedding the rubber block in molten paraffin. According to the method, a smaller number (less than 200) of organoid can be fixed, the damage to the form / structure of the organoid during centrifugation or dissociation is avoided, and the dehydration time is greatly shortened, so that the number and structural integrity of the organoid in the paraffin block are ensured.

The utility model relates to the technical field of experimental appliances, and discloses a paraffin embedding device which comprises a base and a limiting piece sleeved on the base, the base is detachably connected with the limiting piece, an accommodating space is defined when the base is connected with the limiting piece, and the height of the accommodating space is higher than the thickness of a pathological tissue section wrapped by paraffin. After the paraffin embedded with the pathological tissue is formed, the limiting piece exposes the paraffin away from the base along the outer edge of the base; the limiting piece comprises a limiting seat and a limiting piece, the limiting seat is arranged on the base in a sleeving mode, the inner side face of the limiting seat is attached to the outer side face of the base, a limiting groove is formed in the upper surface of the limiting seat, the limiting piece is elastic and can be connected with the limiting groove in a clamped mode, and a connecting piece used for connecting and limiting the two ends of the limiting piece when the limiting piece is enclosed is arranged on the limiting piece; when the limiting piece and the limiting groove are clamped, the limiting piece is cylindrical, and the upper surface of the base, the limiting base and the limiting piece define a containing space. According to the utility model, the frequency of cleaning the operating platform can be reduced, and the wax block can be conveniently taken down.

The utility model relates to the technical field of paraffin embedding devices for organoid samples, and particularly discloses a paraffin embedding and fixing device for organoid tissues, which comprises an embedding box, a frame is arranged on the periphery of the embedding box, a plurality of cavities with the same shape and size are arranged in the frame, and paraffin pouring grooves are arranged among the cavities. An embedding groove is formed in the symmetric center of the embedding box, and a depth groove is formed in the embedding groove; the organ tissue paraffin embedding and fixing device is easy to operate, reasonable in design and convenient to use and maintain, the embedding box can play an effective supporting and fixing role, and damage or deformation caused by misoperation or external force is avoided; due to the design of the wax pouring groove and the embedding groove, the accurate positioning of the position of the tissue block is facilitated, the embedding medium is guided to flow into the embedding groove below in the wax pouring groove, the organ-like tissue block is ensured to be uniformly and tightly wrapped in the embedding medium, and good positioning and guiding effects are achieved.