3 results about "Paraffin embedding" patented technology

This invention provides a method for preparing pathological sections of plague-affected experimental animals. The method includes the following steps: S1: sample pretreatment and fixation; S2: pathogen inactivation verification; S3: tissue dehydration, clearing, and paraffin embedding; S4: paraffin embedding; S5: sectioning and spreading; S6: staining; S7: mounting. The method for preparing pathological sections of plague-affected experimental animals provided by this invention has the advantages of ensuring absolute safety in the preparation process of high-risk pathogen sections through strict biosafety zoning and double inactivation verification, and obtaining high-quality pathological sections.

This invention relates to the field of paraffin sectioning technology and discloses a paraffin section pretreatment method suitable for plant spatial transcriptomics, comprising: (1) sample collection and fixation; (2) sample dehydration; (3) clearing; (4) paraffin embedding; (5) paraffin section preparation; and (6) pretreatment of sections before RNA FISH. This invention can be widely applied to various plant tissues, and its detection sensitivity can reach the visualization of low-expression genes (FPKM≤20), contributing to the popularization and development of plant spatial transcriptomics.

The application discloses pathological tissue paraffin embedding device, relates to the technical field of medical devices, and relates to embedding forceps, a pressing block and a temperature controller, wherein the embedding forceps comprise a forceps handle and two forceps arms; the two forceps arms are in heat conduction connection with the forceps handle; the pressing block comprises a flattening end face, the flattening end face is used for flattening pathological tissue, one side of the pressing block, which is away from the flattening end face, is detachably connected with an end of the forceps handle, which is away from the forceps arms, and the pressing block and the forceps handle are in heat conduction contact in the connected state; and the temperature controller is in heat conduction connection with the forceps handle through a heat conduction wire. Wherein the flattening end face is provided with a first anti-sticking layer with micro-texture, and the pressing block is provided with exhaust grooves on the peripheral side. In the application, the pressing block is detachably connected with the embedding forceps, the embedding forceps can be rotated by 180 degrees to be operated by using the pressing block, the operation is simple and convenient, and the working efficiency can be improved; and the embedding forceps and the temperature controller are designed to be separated, are connected through the heat conduction wire, the embedding forceps are simple in structure, long in service life and low in maintenance cost.