882 results about "Experimental animal" patented technology

The invention discloses a self-replicating RNA (Ribonucleic Acid) molecule, a porcine rotavirus RNA vaccine and application thereof, and relates to the technical field of vaccines. According to the present invention, the coding sequence of the porcine rotavirus G9P23 type VP4 protein is optimized, such that the fact that the saRNA prepared by using the nucleotide sequence represented by the SEQ ID NO: 10 can secrete the high porcine rotavirus G9P23 type VP4 protein antigen after the cell transfection is found; after the optimized porcine rotavirus G9P23 type VP4 protein is used for immunizing experimental animals, the level of neutralizing antibodies generated by the animals can be improved, and the challenge protection effect is enhanced. Therefore, the nucleic acid molecule provided by the invention can be used for developing drugs and vaccines for causing protective immune response in experimental animal bodies. Compared with a traditional vaccine, the vaccine provided by the invention has good immunogenicity and safety, and has a good application prospect.

The invention belongs to the technical field of blood detection, and particularly relates to an experimental animal blood detection device which comprises a detector body and a sample injection needle, and further comprises a sample mixing part, and the sample mixing part comprises a disc rotationally installed on the detector body; the fixing bases are installed on the disc, and clamping assemblies are rotationally installed on the fixing bases; the driving assembly is installed on the fixing base and comprises a main shaft rotationally installed on the fixing base and an auxiliary shaft used for driving the clamping jaw to rotate, a plurality of sets of auxiliary gears and main gears are installed on the auxiliary shaft and the main shaft respectively, the tooth ratios of the auxiliary gears and the main gears in each set are different, and the auxiliary shaft is of a telescopic structure; by arranging the driving assembly, different shaking speeds are adopted for blood collection tubes of different specifications, it is ensured that blood and an anticoagulant are evenly mixed, the situation of hemolysis or uneven mixing is avoided, and the detection accuracy and efficiency are improved.

The invention discloses a technology for targeted stimulation of a specific brain region and application of the technology in regulation and control of brain functions, and belongs to the field of animal cognitive behavior research. Techniques for targeted stimulation of specific brain regions include insulated stimulation electrodes that can generate AC voltage stimulation and a stimulation device for adjusting the AC voltage intensity and frequency. By applying the technology (ntTMS) for targeted stimulation of the specific brain region, an obvious magnetic stimulation effect can be achieved in the center of the brain region, cerebral neurons of a target region are activated, social and cognitive behaviors of animals are effectively regulated and controlled, and no obvious side effect is brought to experimental animals.

The invention relates to the technical field of extracranial strike testing, in particular to digitally-controlled extracranial strike construction equipment of a traumatic brain injury model. Comprising a test platform, a sliding rail installed on the test platform and arranged in the length direction of the test platform, an animal fixing platform slidably connected with the test platform through the sliding rail, an impact structure arranged on one side of the test platform and a driving structure used for driving the animal fixing platform to move towards the impact structure. The impact structure is fixedly connected to the test platform, the output end of the impact structure faces the animal fixing platform, an experimental animal is installed on the animal fixing platform, the driving structure drives the animal fixing platform to enable the experimental animal to actively impact, and the impact structure enables the experimental animal to impact a fixed acting point at a certain initial speed. The system is more in line with the injury mechanism of a patient with clinical actual craniocerebral trauma, and a tester can accurately control and monitor parameters according to the moving speed and the moving distance of the animal fixing platform.

The invention provides construction and application of a human pluripotent stem cell-derived heart organoid traditional Chinese medicine extract toxicity test chip. The chip comprises a plurality of micro-cell culture chambers and a micro-fluidic pore channel, heart organoid culture is carried out in the micro-cell culture room, culture media are added in the micro-fluidic channel to maintain the activity of the heart organoid, and traditional Chinese medicine extracts are added to evaluate the toxicity of traditional Chinese medicine. By simulating the reaction of the heart organ, the toxicity of the traditional Chinese medicine can be evaluated, the safety and effectiveness information about the traditional Chinese medicine can be provided, finally, the use of experimental animals can be reduced, and the animal welfare can be improved.

The invention provides a device and equipment for evaluating the dose-effect relationship of craniocerebral injury caused by human body explosive shock waves and a medium, and relates to the technical field of craniocerebral injury dose-effect relationship evaluation.The method comprises the steps that firstly, the scale law relationship of explosive shock wave attenuation coefficients of different species is established, and the incidence pressure peak values of the forehead of the human body corresponding to different incidence overpressure duration time are calculated; and then establishing a human body craniocerebral explosion injury 50% survival curve, and evaluating the craniocerebral injury dose-effect relationship of the person to be evaluated based on the curve. According to the evaluation device, by combining experimental animals with different weights and skull thicknesses, high-precision physical head models and other outfield actual explosion experiments, the dose-effect relationship of the craniocerebral injury of the experimental animals is transited to people, the dose-effect relationship of the craniocerebral injury of the human body is established, a craniocerebral injury evaluation system of the human body explosive shock waves is formed, and the complex structure of the craniocerebral of the human body is considered; and the dose-effect relationship of the animal is filtered into the human body, so that the dose-effect relationship of craniocerebral injury when the human body is subjected to explosion impact can be evaluated more accurately.

The invention discloses a humanized osteoporosis mouse model and a construction method thereof, and belongs to the technical field of biotechnology and animal models. The invention aims at the core defects of large species difference, insufficient humanization degree, low pathological simulation degree, unstable phenotype and the like of the existing osteoporosis model. The humanized osteoporosis mouse model is constructed through five core steps of experimental animal pretreatment, collagen-nano hydroxyapatite bionic scaffold preparation and cell pre-implantation, immune-bone metabolism double-derived system construction, progressive osteoporosis induction and model identification and verification. According to the method, double-person origination of immune and bone metabolism systems is realized for the first time, the colonization rate and survival time of human cells are greatly improved, the pathological process of clinical postmenopausal osteoporosis is accurately simulated, the phenotype of the model is stable and uniform, the reactivity matching degree with clinical drugs is high, and the method can be widely applied to research and development of anti-osteoporosis drugs and research of pathological mechanisms.

The invention relates to a second antibody for resisting mouse IgG and rabbit IgG as well as a preparation method and application of the second antibody. The second antibody of the anti-mouse IgG and the rabbit IgG sequentially comprises an anti-mouse IgG nano antibody, a biotin fixed-point binding peptide fragment and an anti-rabbit IgG nano antibody from an amino terminal to a carboxyl terminal. According to the design of the second antibody of the anti-mouse IgG and rabbit IgG with a brand-new structure and the preparation method, high-efficiency expression is realized, the second antibody of the anti-mouse IgG and rabbit IgG capable of realizing fixed-point biotinylation is obtained, the second antibody can be further subjected to various modification reformation based on a biotin-streptavidin bridge, the preparation method of the second antibody of the polymerase labeled anti-mouse IgG and rabbit IgG is further developed, and the application of the second antibody of the polymerase labeled anti-mouse IgG and rabbit IgG is expanded. According to the method, the polymerase-labeled mouse-rabbit universal secondary antibody with similar affinity to mouse IgG and rabbit IgG is obtained, the preparation period of the secondary antibody can be shortened, the preparation process of the secondary antibody is simplified, the stability between batches is improved, and meanwhile, the dependence on experimental animals is reduced.

The invention relates to a heptafluoropropane fire extinguishing system for an experimental animal barrier facility and a use method thereof, the heptafluoropropane fire extinguishing system comprises a plurality of smoke detectors arranged on a ceiling of the experimental animal barrier facility, and the smoke detectors are equidistantly arranged into a two-dimensional array formed by a plurality of square units; the plurality of heptafluoropropane nozzles are arranged in the centers of the square units, and an HEPA filter is mounted in each heptafluoropropane nozzle; each smoke detector is internally provided with at least one smoke collecting port facing the adjacent heptafluoropropane nozzle and a smoke sensor; the at least one heptafluoropropane gas cylinder is communicated with the heptafluoropropane spray head through conveying pipes, and each conveying pipe is provided with an electromagnetic valve; and the control unit is connected to the smoke sensor and the electromagnetic valves, and is configured to position a fire position according to a signal of the smoke sensor and control the corresponding electromagnetic valves to be opened, so that heptafluoropropane is sprayed through the adjacent heptafluoropropane spray heads to carry out local fire extinguishment.

The invention relates to the technical field of animal experiments, and particularly discloses an experimental animal PET-CT examination auxiliary fixing device which comprises a fixing base, the upper surface of the fixing base is fixedly connected with a first supporting column, the upper surface of the first supporting column is fixedly connected with a first limiting plate, and the side surface of the first limiting plate is fixedly connected with two supporting limiting blocks. The experimental animal PET-CT examination auxiliary fixing device is provided with a supporting limiting block and a positioning sliding rail, when the experimental animal PET-CT examination auxiliary fixing device works, by sliding a second supporting column, a transmission rolling wheel at the lower end of the second supporting column is used for supporting, friction force is dropped through rotation of the transmission rolling wheel, and therefore practicability is improved; and a second supporting column is conveniently guided, so that the movement stability of the second supporting column is improved, the distance between a first limiting plate and a second limiting plate is conveniently changed, and the convenience of fixing different rabbits is improved.

The invention discloses a drug screening method based on endotoxin attack, and relates to the technical field of drug screening, in the process of drug screening by using an experimental animal, firstly, an experimental animal model with diseases is established, a certain dose of endotoxin is given to the experimental animal for a period of time after the model is successfully built, and then the experimental animal is subjected to drug screening. And establishing evaluation indexes by utilizing a mathematical formula, and comprehensively evaluating the effectiveness and the safety of the medicine. The evaluation indexes comprise an endotoxin attack survival rate, a survival disease control rate and a survival disease control index. The method can be applied to screening of drugs for treating different diseases, and a more scientific and reliable screening method is provided for research of preclinical drugs or treatment methods.

The utility model discloses an experimental animal feed double-layer vacuum package automatic pushing device which comprises a first conveyor, a second conveyor, a lifting platform, a pushing assembly, a third conveyor, a grabbing assembly, a fourth conveying mechanism, a sealing mechanism and a fifth conveying mechanism which are sequentially arranged. Wherein two groups of the first conveyor, the second conveyor, the third conveyor, the fourth conveying mechanism, the sealing mechanism and the fifth conveying mechanism are arranged side by side. According to the double-layer vacuum shaping packaging machine, double-layer vacuum shaping packaging is met, automatic bag feeding, bag pushing and bagging and vacuumizing sealing of a second-layer packaging bag are achieved, the steps can be completed in one device, the occupied space is saved, and the packaging efficiency is improved; meanwhile, the double-layer packaging bag achieves independent vacuum packaging, double protection is achieved, contamination of microorganisms in product contents is avoided, a flat packaging form is formed, the risk probability of bumping and bag breaking is reduced, and the bag breaking protection effect is improved.

The invention discloses an establishment method and application of a damp-heat intestine accumulation type ulcerative colitis animal model, and belongs to the field of animal models.The establishment method comprises the steps that S1, high-fat feed treatment is conducted, specifically, experimental animals freely eat high-fat feed for 6-10 weeks from the first day; s2, DSS treatment: feeding 1.5% (w / v) DSS solution to the experimental animal for 2 weeks; and S3, observing the damp-heat syndrome type and ulcerative colitis related indications of the experimental animals, and selecting the experimental animals meeting the scoring standard, thereby establishing the ulcerative colitis mouse model. Aiming at the situation that most patients induce ulcerative colitis due to excess energy due to the change of dietary structures and lifestyles at present, the invention constructs and evaluates the damp-heat accumulation type ulcerative colitis mouse model by inducing internal dampness by a direct pathogenesis (high fat diet), which is closer to the actual situation of clinical patients. Therefore, a model and reference are provided for research and treatment of pathology of the damp-heat accumulation type ulcerative colitis and pharmacological mechanisms of traditional Chinese medicines.

The invention belongs to the technical field of experimental zoology, and particularly relates to a gastric cancer precancerous lesion animal model and a construction method and application thereof. The construction method of the gastric precancerous lesion animal model comprises the following steps that a modeling animal freely drinks a modeling agent solution every day for modeling, and the concentration of the modeling agent solution is increased in a gradient mode within 200 mg / L-800 mg / L along with the modeling time. The gastric cancer precancerous lesion animal model is constructed by the method. The gastric precancerous lesion animal model is applied to screening, research and development of traditional Chinese medicines for treating gastric precancerous lesions including chronic atrophic gastritis, intestinal metaplasia and dysplasia. The method can solve the technical problem that the existing gastric precancerous lesion animal model construction method cannot accurately simulate the disease stage evolution, can simply, conveniently and efficiently construct the gastric precancerous lesion model, and has important meanings for scientific research verification, drug research and development, quality control, clinical application and the like of traditional Chinese medicines or formulae for treating gastric precancerous lesions.

The invention relates to the technical field of animal experiment devices, and discloses a spatial cognition enhancement device and method for three-dimensional rotational stimulation of a vestibular-hippocampus pathway, and the device comprises a rotational stimulation unit which is used for generating multi-axial angular acceleration stimulation; the animal adaptation unit is connected with the rotary stimulation unit and is used for fixing an experimental animal and blocking visual input; the control unit is in signal connection with the rotary stimulation unit and is used for adjusting stimulation parameters and collecting dynamic data; and the signal acquisition unit comprises a piezoresistive sensor and an analog-to-digital converter and is used for acquiring kinetic parameters of angular velocity and angular acceleration in real time. According to the space cognition enhancement device and method for three-dimensional rotation stimulation of the vestibular-hippocampus pathway, real three-dimensional space motion is simulated through multi-axial rotation, and targeted stimulation of the vestibular-hippocampus pathway is achieved in combination with adjustable rotating speed and visual shielding maintenance. The device is integrated with an acoustic intervention module and supports multi-mode intervention.

The invention discloses a construction method and application of a depression model. A drug capable of activating neurons in the lateral habenular nucleus brain area to discharge is given to the lateral habenular nucleus area of the brain of the experimental animal, and the depression model is obtained. The invention develops a new method for establishing the depression animal model by utilizing drug induction, local drug administration is performed on the brain of an experimental animal, abnormal discharge activity of neurons of lateral habenula nucleus (LHB) is activated, depression-like behaviors are generated, and the depression-like behaviors are induced to be more stable. Therefore, a new and reliable depression animal model is provided for researching the brain mechanism of depression and researching and developing novel drugs and non-drug intervention methods.

The invention belongs to the field of biological medicine research and development, discloses a method and a system for collecting and analyzing auditory electrophysiological data of experimental animals, and is particularly suitable for non-human primates. The method comprises the following steps: delivering a drug to a middle ear cavity through an external auditory canal, and synchronously recording auditory electrophysiological data before and after drug administration; aBR and DPOAE multi-mode detection is adopted, and the frequency range of 4 kHz to 32 kHz is covered; setting anesthesia depth and three-electrode positioning to reduce interference; optimizing the signal-to-noise ratio through noise suppression and dynamic signal superposition; an auditory threshold database is constructed, and a machine learning model is combined to judge an injured part. The system integrates a multi-modal stimulation module, a minimally invasive drug delivery device and a data analysis platform, and supports cross-species data compatibility. The accuracy of otology medicine research is remarkably improved, the surgical operation risk is avoided, and the method is suitable for gene therapy, ototoxicity screening and other scenes.

The invention discloses an adjustable spinal cord impact vibration injury device and an injury method thereof, and belongs to the technical field of spinal cord injury experiment research. Two lifting sliding rods are fixedly installed at the rear end of the base, and lifting sliding blocks capable of vertically sliding are installed on the lifting sliding rods. An angle adjusting mechanism is installed on the lifting sliding block, the movable end of the angle adjusting mechanism is detachably connected with a supporting tool used for fixing the experimental animal, and the angle adjusting mechanism is used for adjusting the angle of the supporting tool so that different parts of the experimental animal can face the base; the base is further provided with an impact triggering mechanism. Through the mode, the experimental animal spinal cord impact oscillation injury model prepared by adopting the technical scheme can well simulate the injury process of a clinical spinal cord injury patient and the pathophysiological change after injury; and the spinal cord impact vibration injury models with different injury modes and different injury degrees are prepared by changing the free falling body height of the experimental animal, the vertical impact rods made of different materials and the impact point location of the experimental animal.

The invention provides a Wuzhishan pig organ multi-omics data space integration and analysis method, and belongs to the technical field of data statistics and analysis, the method comprises the following steps: S1, experiment design and Wuzhishan pig organ sample standardization preparation; s2, independently collecting and preprocessing multi-omics data of Wuzhishan pig organs; s3, multi-omics data standardization and batch effect correction; s4, labeling and calibrating spatial dimension information; s5, spatial specificity correlation modeling of the multi-omics data is carried out; s6, carrying out integrated clustering analysis on the spatial multi-omics data; s7, performing function annotation and path enrichment analysis on the clustering feature clusters; and S8, analyzing the spatial specific molecular mechanism and constructing a multi-omics data spatial integration database. According to the method, spatialization and systematization analysis of the Wuzhishan pig organ multi-omics data is achieved through collaborative optimization of the HHO-IK-means-BI three algorithms, and technical support is provided for experimental zoology research and conversion of medical application.

The invention relates to the field of medical biology, and discloses a construction method and device of a bipolar disorder experimental animal model and application, the construction method comprises the following steps: selecting a healthy experimental animal to carry out environmental adaptation feeding before modeling, then carrying out sleep deprivation stimulation for 3-5 days continuously, and carrying out sleep deprivation stimulation for 15-18 hours continuously and 6-9 hours for rest every day so as to freely eat and feed water; and after the sleep deprivation stimulation is finished, evaluating the surface validity of the disease model for the experimental animal by adopting behavioral test. The construction method provided by the invention is simple to operate and easy to repeat, can better simulate the disease of a clinical bipolar disorder patient, and provides a new model normal form for pathogenesis research and clinical drug screening of the bipolar disorder, and the disease model can be maintained for a long time.

The invention relates to the technical field of animal disease models, and discloses a construction method of an intraocular hypertension animal model, comprising the following steps: step A, selecting an experimental animal; step B, preparing experimental instruments and reagents; c, in an anesthesia mode, deep anesthesia is carried out on the animal, and subsequent deep anesthesia is carried out; d, intraocular pressure measurement, wherein the basic intraocular pressure value is measured through anterior chamber injection. Step E, performing anterior chamber injection, and using ofloxacin antibiotic oculentum to prevent infection after an operation; and F, intraocular pressure measurement: measuring the change of the intraocular pressure value after the anterior chamber injection. According to the method, the natural pathological process of open-angle glaucoma is simulated by injecting IFN-beta into the anterior chamber, the situation that acute intraocular pressure rise is caused mainly by blocking an aqueous humor flow outlet channel like other models is avoided, and the method can better reflect the real situation of glaucoma diseases; in addition, the model avoids intraocular pressure change caused by anatomical structure change, so that the accuracy and reliability of the model are improved.

The utility model discloses a rearing type double-layer experimental animal isolator, and aims to solve the problems that a traditional single-layer rearing isolator is small in animal breeding quantity, large in occupied area and high in labor intensity due to manual excrement removal. The isolator adopts a double-layer box body structure and comprises an upper-layer box body and a lower-layer box body which are communicated with each other, and excrement receiving devices and air conditioning systems are arranged at the bottoms of the isolator. The excrement receiving device is composed of an excrement receiving disc and an excrement scraping device, and excrement is automatically cleaned to the excrement collecting box through an excrement scraping plate. A feces discharge device is arranged in the feces collection box, and a stirring paddle and an electric valve are matched with a concentric-square-shaped bent pipe, so that feces can be discharged in time, and manual intervention is reduced. The air conditioning system adopts an independent air supply system and an air exhaust system, an air supply outlet and an air exhaust outlet are optimally designed to ensure uniform airflow, the air supply outlet and the air exhaust outlet are diagonally arranged, an upper-supply and lower-return air supply mode is adopted, the air supply outlet adopts a static pressure pore plate to supply air, and the air conditioning system has the characteristics of uniform air supply, low noise and small blowing feeling to animals, and provides a good raising environment for the animals.

The invention discloses an animal blood pressure prediction model establishing method based on smell information. The animal blood pressure prediction model establishing method comprises the following steps that response signals are obtained through response of an electronic nose gas sensor array to feces smell of a hypertensive rat; detecting the blood pressure of the hypertensive rat of which the excrement smell is detected; extracting characteristic response signals from the excrement smell information, wherein the characteristic response signals comprise a response mean value, a steady-state value, an integral and a differential; principal component analysis and discriminant analysis are carried out on the extracted characteristic values, and qualitative discrimination is carried out in combination with the blood pressure states (hypertension and normal blood pressure) of the rats; the extracted characteristic values serve as independent variables, multivariate linear analysis, a multi-layer perceptron neural network and partial least squares regression analysis are carried out, and quantitative prediction is carried out on the blood pressure of the female rats and the male rats. By means of the method, rat blood pressure state judgment and blood pressure detection based on excrement smell are achieved, and the problems that invasive operation difficulty is large, conscious animal individual compliance is poor, external interference factors are large, and a large amount of manpower, material resources and financial resources are consumed are solved. And a basis is provided for rapid and non-invasive evaluation of experimental animals.

A method for animal identification and tracking includes, during a dark cycle: illuminating an interior of an animal cage with light outside of a visible light range; and capturing a first image of the interior of the animal cage. The method also includes: detecting a first identification tag in the first image; extracting a first set of tag features from the first image; identifying a first animal identifier of a first animal, tagged with the first identification tag, based on the first set of tag features; interpreting a first animal position of the first animal occupying the animal cage based on the first image; appending a cage log with the first animal identifier, a first timestamp corresponding to a time of capture of the first image, and the first animal position.

The invention provides a Garlu virus attenuated strain and application thereof, and relates to the field of virology and biological medicine. According to the invention, the screened Garlu virus attenuated strain is a natural attenuated strain derived from a tick sample, can grow in Vero cells, has the titer of more than or equal to 107 TCID50 / mL, does not have clinical pathogenicity to an immunodeficient host, and is safe; after the attenuated strain is used as an immunogen for pre-intervention, infection of a lethal virus strain can be avoided, the survival rate is increased to 100%, adverse effects on growth and life of experimental animals are avoided, particularly, a host can be stimulated to generate humoral immunity and cellular immunity at the same time, and the survival rate of the experimental animals is improved. And moreover, broad-spectrum cross-protection efficacy can be generated on Bandavirus lethal virus strains, and a foundation is laid for improvement of research on Bandavirus live vaccines such as the Colu virus and the like.

Provided are a lipid nanoparticle composition for delivering a nucleic acid drug, a preparation method therefor, and use thereof. The lipid nanoparticle composition comprises three lipid components: a steroid-cationic lipid compound, a neutral phospholipid, and a polyethylene glycol lipid. The composition prepared by mixing the described components has relatively good stability and transfection efficiency. The lipid nanoparticle is used for delivering nucleic acid, such as mRNA, can efficiently and stably deliver the nucleic acid drug to a target cell or organ, and can induce a relatively high specific antibody response in an experimental animal, and the antibody has a better safety profile.

The invention discloses a construction method of a zebra fish reproductive toxicity model, a method for evaluating reproductive toxicity of chemical substances and application, and relates to the technical field of reproductive development toxicity of chemical substances. According to the method for evaluating the reproductive toxicity of the chemical substance provided by the invention, the change of a diatrix behavior can be concerned by shooting behavioral pictures or videos without sacrifice of an experimental animal or professional fluorescence imaging instrument equipment and tissue slicing equipment; the method is more in line with animal ethics, cost is saved, and continuous observation can be realized by using the same batch of organisms. The test courtship behavior can quickly utilize software to analyze and obtain zebra fish behavior changes. By adopting the evaluation method provided by the invention, both female ovarian malformation and male reproductive toxicity can be detected, and the evaluation method has the advantage of more evaluation dimensions. Based on the method provided by the invention, a zebrafish reproductive toxicity model can be constructed, and the model can be used as a drug screening platform for reproductive function damage of zebrafish.

The invention discloses a multi-level access control and privacy protection-oriented experimental animal ethical auditing management system, and particularly relates to the field of experimental animal ethical auditing, which comprises an authority access module, a semantic modeling module, an expert matching module, an auditing closed loop module, a compliance filing module and a strategy evolution module. A bidirectional semantic path alignment mechanism between a high-order index tensor driven by semantic modeling and an expert portrait semantic graph is constructed, and authority hierarchical control, an auditing feedback closed loop and a strategy evolution scheduling model are combined; automatic and structured management of information extraction, expert distribution and feedback processing in the whole process of experimental animal ethical application is realized, so that the problems of manual processing lag, insufficient matching accuracy, data non-traceability and the like in the existing examination process are effectively solved.

The invention relates to the technical field of pathogenic microorganism detection, and particularly discloses a primer group, a detection kit and a detection method for detecting pathogenic microorganisms of rodent experimental animals. The primer group disclosed by the invention is used for simultaneously detecting pasteurella pneumophila, klebsiella pneumoniae and staphylococcus aureus; the primer group comprises a primer pair aiming at pasteurella pneumophila, and the sequences of the primer pair are SEQ ID NO: 1 and SEQ ID NO: 2; the sequences of the primer pair aiming at the klebsiella pneumoniae are SEQ ID NO: 7 and SEQ ID NO: 8; the sequences of the primer pair aiming at the staphylococcus aureus are SEQ ID NO: 9 and SEQ ID NO: 10. The primer group and the detection method disclosed by the invention can realize rapid detection of three bacteria on a molecular level, have the advantages of simplicity and convenience in operation, high detection efficiency and accurate result, and are suitable for rapid screening and monitoring of health conditions of experimental animals.

The invention relates to the technical field of biological medicine, in particular to a construction method and application of a smog disease animal model. The construction method comprises the following steps: (1) carrying out bilateral carotid artery ligation on an experimental animal; and (2) feeding the ligated animals in a low-oxygen environment to obtain the smog disease animal model. According to the construction method provided by the invention, the stable smog disease animal model can be constructed, and the construction method is simple, reliable and easy to implement.