672 results about "Experimental animal" patented technology

The invention discloses a self-replicating RNA (Ribonucleic Acid) molecule, a porcine rotavirus RNA vaccine and application thereof, and relates to the technical field of vaccines. According to the present invention, the coding sequence of the porcine rotavirus G9P23 type VP4 protein is optimized, such that the fact that the saRNA prepared by using the nucleotide sequence represented by the SEQ ID NO: 10 can secrete the high porcine rotavirus G9P23 type VP4 protein antigen after the cell transfection is found; after the optimized porcine rotavirus G9P23 type VP4 protein is used for immunizing experimental animals, the level of neutralizing antibodies generated by the animals can be improved, and the challenge protection effect is enhanced. Therefore, the nucleic acid molecule provided by the invention can be used for developing drugs and vaccines for causing protective immune response in experimental animal bodies. Compared with a traditional vaccine, the vaccine provided by the invention has good immunogenicity and safety, and has a good application prospect.

The invention discloses a technology for targeted stimulation of a specific brain region and application of the technology in regulation and control of brain functions, and belongs to the field of animal cognitive behavior research. Techniques for targeted stimulation of specific brain regions include insulated stimulation electrodes that can generate AC voltage stimulation and a stimulation device for adjusting the AC voltage intensity and frequency. By applying the technology (ntTMS) for targeted stimulation of the specific brain region, an obvious magnetic stimulation effect can be achieved in the center of the brain region, cerebral neurons of a target region are activated, social and cognitive behaviors of animals are effectively regulated and controlled, and no obvious side effect is brought to experimental animals.

The invention discloses a humanized osteoporosis mouse model and a construction method thereof, and belongs to the technical field of biotechnology and animal models. The invention aims at the core defects of large species difference, insufficient humanization degree, low pathological simulation degree, unstable phenotype and the like of the existing osteoporosis model. The humanized osteoporosis mouse model is constructed through five core steps of experimental animal pretreatment, collagen-nano hydroxyapatite bionic scaffold preparation and cell pre-implantation, immune-bone metabolism double-derived system construction, progressive osteoporosis induction and model identification and verification. According to the method, double-person origination of immune and bone metabolism systems is realized for the first time, the colonization rate and survival time of human cells are greatly improved, the pathological process of clinical postmenopausal osteoporosis is accurately simulated, the phenotype of the model is stable and uniform, the reactivity matching degree with clinical drugs is high, and the method can be widely applied to research and development of anti-osteoporosis drugs and research of pathological mechanisms.

The invention discloses a drug screening method based on endotoxin attack, and relates to the technical field of drug screening, in the process of drug screening by using an experimental animal, firstly, an experimental animal model with diseases is established, a certain dose of endotoxin is given to the experimental animal for a period of time after the model is successfully built, and then the experimental animal is subjected to drug screening. And establishing evaluation indexes by utilizing a mathematical formula, and comprehensively evaluating the effectiveness and the safety of the medicine. The evaluation indexes comprise an endotoxin attack survival rate, a survival disease control rate and a survival disease control index. The method can be applied to screening of drugs for treating different diseases, and a more scientific and reliable screening method is provided for research of preclinical drugs or treatment methods.

The utility model discloses an experimental animal feed double-layer vacuum package automatic pushing device which comprises a first conveyor, a second conveyor, a lifting platform, a pushing assembly, a third conveyor, a grabbing assembly, a fourth conveying mechanism, a sealing mechanism and a fifth conveying mechanism which are sequentially arranged. Wherein two groups of the first conveyor, the second conveyor, the third conveyor, the fourth conveying mechanism, the sealing mechanism and the fifth conveying mechanism are arranged side by side. According to the double-layer vacuum shaping packaging machine, double-layer vacuum shaping packaging is met, automatic bag feeding, bag pushing and bagging and vacuumizing sealing of a second-layer packaging bag are achieved, the steps can be completed in one device, the occupied space is saved, and the packaging efficiency is improved; meanwhile, the double-layer packaging bag achieves independent vacuum packaging, double protection is achieved, contamination of microorganisms in product contents is avoided, a flat packaging form is formed, the risk probability of bumping and bag breaking is reduced, and the bag breaking protection effect is improved.

The invention belongs to the technical field of experimental zoology, and particularly relates to a gastric cancer precancerous lesion animal model and a construction method and application thereof. The construction method of the gastric precancerous lesion animal model comprises the following steps that a modeling animal freely drinks a modeling agent solution every day for modeling, and the concentration of the modeling agent solution is increased in a gradient mode within 200 mg / L-800 mg / L along with the modeling time. The gastric cancer precancerous lesion animal model is constructed by the method. The gastric precancerous lesion animal model is applied to screening, research and development of traditional Chinese medicines for treating gastric precancerous lesions including chronic atrophic gastritis, intestinal metaplasia and dysplasia. The method can solve the technical problem that the existing gastric precancerous lesion animal model construction method cannot accurately simulate the disease stage evolution, can simply, conveniently and efficiently construct the gastric precancerous lesion model, and has important meanings for scientific research verification, drug research and development, quality control, clinical application and the like of traditional Chinese medicines or formulae for treating gastric precancerous lesions.

The invention relates to the technical field of animal experiment devices, and discloses a spatial cognition enhancement device and method for three-dimensional rotational stimulation of a vestibular-hippocampus pathway, and the device comprises a rotational stimulation unit which is used for generating multi-axial angular acceleration stimulation; the animal adaptation unit is connected with the rotary stimulation unit and is used for fixing an experimental animal and blocking visual input; the control unit is in signal connection with the rotary stimulation unit and is used for adjusting stimulation parameters and collecting dynamic data; and the signal acquisition unit comprises a piezoresistive sensor and an analog-to-digital converter and is used for acquiring kinetic parameters of angular velocity and angular acceleration in real time. According to the space cognition enhancement device and method for three-dimensional rotation stimulation of the vestibular-hippocampus pathway, real three-dimensional space motion is simulated through multi-axial rotation, and targeted stimulation of the vestibular-hippocampus pathway is achieved in combination with adjustable rotating speed and visual shielding maintenance. The device is integrated with an acoustic intervention module and supports multi-mode intervention.

The invention belongs to the field of biological medicine research and development, discloses a method and a system for collecting and analyzing auditory electrophysiological data of experimental animals, and is particularly suitable for non-human primates. The method comprises the following steps: delivering a drug to a middle ear cavity through an external auditory canal, and synchronously recording auditory electrophysiological data before and after drug administration; aBR and DPOAE multi-mode detection is adopted, and the frequency range of 4 kHz to 32 kHz is covered; setting anesthesia depth and three-electrode positioning to reduce interference; optimizing the signal-to-noise ratio through noise suppression and dynamic signal superposition; an auditory threshold database is constructed, and a machine learning model is combined to judge an injured part. The system integrates a multi-modal stimulation module, a minimally invasive drug delivery device and a data analysis platform, and supports cross-species data compatibility. The accuracy of otology medicine research is remarkably improved, the surgical operation risk is avoided, and the method is suitable for gene therapy, ototoxicity screening and other scenes.

The invention provides a Wuzhishan pig organ multi-omics data space integration and analysis method, and belongs to the technical field of data statistics and analysis, the method comprises the following steps: S1, experiment design and Wuzhishan pig organ sample standardization preparation; s2, independently collecting and preprocessing multi-omics data of Wuzhishan pig organs; s3, multi-omics data standardization and batch effect correction; s4, labeling and calibrating spatial dimension information; s5, spatial specificity correlation modeling of the multi-omics data is carried out; s6, carrying out integrated clustering analysis on the spatial multi-omics data; s7, performing function annotation and path enrichment analysis on the clustering feature clusters; and S8, analyzing the spatial specific molecular mechanism and constructing a multi-omics data spatial integration database. According to the method, spatialization and systematization analysis of the Wuzhishan pig organ multi-omics data is achieved through collaborative optimization of the HHO-IK-means-BI three algorithms, and technical support is provided for experimental zoology research and conversion of medical application.

The invention relates to the field of medical biology, and discloses a construction method and device of a bipolar disorder experimental animal model and application, the construction method comprises the following steps: selecting a healthy experimental animal to carry out environmental adaptation feeding before modeling, then carrying out sleep deprivation stimulation for 3-5 days continuously, and carrying out sleep deprivation stimulation for 15-18 hours continuously and 6-9 hours for rest every day so as to freely eat and feed water; and after the sleep deprivation stimulation is finished, evaluating the surface validity of the disease model for the experimental animal by adopting behavioral test. The construction method provided by the invention is simple to operate and easy to repeat, can better simulate the disease of a clinical bipolar disorder patient, and provides a new model normal form for pathogenesis research and clinical drug screening of the bipolar disorder, and the disease model can be maintained for a long time.

The utility model discloses a rearing type double-layer experimental animal isolator, and aims to solve the problems that a traditional single-layer rearing isolator is small in animal breeding quantity, large in occupied area and high in labor intensity due to manual excrement removal. The isolator adopts a double-layer box body structure and comprises an upper-layer box body and a lower-layer box body which are communicated with each other, and excrement receiving devices and air conditioning systems are arranged at the bottoms of the isolator. The excrement receiving device is composed of an excrement receiving disc and an excrement scraping device, and excrement is automatically cleaned to the excrement collecting box through an excrement scraping plate. A feces discharge device is arranged in the feces collection box, and a stirring paddle and an electric valve are matched with a concentric-square-shaped bent pipe, so that feces can be discharged in time, and manual intervention is reduced. The air conditioning system adopts an independent air supply system and an air exhaust system, an air supply outlet and an air exhaust outlet are optimally designed to ensure uniform airflow, the air supply outlet and the air exhaust outlet are diagonally arranged, an upper-supply and lower-return air supply mode is adopted, the air supply outlet adopts a static pressure pore plate to supply air, and the air conditioning system has the characteristics of uniform air supply, low noise and small blowing feeling to animals, and provides a good raising environment for the animals.

The invention provides a Garlu virus attenuated strain and application thereof, and relates to the field of virology and biological medicine. According to the invention, the screened Garlu virus attenuated strain is a natural attenuated strain derived from a tick sample, can grow in Vero cells, has the titer of more than or equal to 107 TCID50 / mL, does not have clinical pathogenicity to an immunodeficient host, and is safe; after the attenuated strain is used as an immunogen for pre-intervention, infection of a lethal virus strain can be avoided, the survival rate is increased to 100%, adverse effects on growth and life of experimental animals are avoided, particularly, a host can be stimulated to generate humoral immunity and cellular immunity at the same time, and the survival rate of the experimental animals is improved. And moreover, broad-spectrum cross-protection efficacy can be generated on Bandavirus lethal virus strains, and a foundation is laid for improvement of research on Bandavirus live vaccines such as the Colu virus and the like.

The invention discloses a multi-level access control and privacy protection-oriented experimental animal ethical auditing management system, and particularly relates to the field of experimental animal ethical auditing, which comprises an authority access module, a semantic modeling module, an expert matching module, an auditing closed loop module, a compliance filing module and a strategy evolution module. A bidirectional semantic path alignment mechanism between a high-order index tensor driven by semantic modeling and an expert portrait semantic graph is constructed, and authority hierarchical control, an auditing feedback closed loop and a strategy evolution scheduling model are combined; automatic and structured management of information extraction, expert distribution and feedback processing in the whole process of experimental animal ethical application is realized, so that the problems of manual processing lag, insufficient matching accuracy, data non-traceability and the like in the existing examination process are effectively solved.

The invention relates to the technical field of pathogenic microorganism detection, and particularly discloses a primer group, a detection kit and a detection method for detecting pathogenic microorganisms of rodent experimental animals. The primer group disclosed by the invention is used for simultaneously detecting pasteurella pneumophila, klebsiella pneumoniae and staphylococcus aureus; the primer group comprises a primer pair aiming at pasteurella pneumophila, and the sequences of the primer pair are SEQ ID NO: 1 and SEQ ID NO: 2; the sequences of the primer pair aiming at the klebsiella pneumoniae are SEQ ID NO: 7 and SEQ ID NO: 8; the sequences of the primer pair aiming at the staphylococcus aureus are SEQ ID NO: 9 and SEQ ID NO: 10. The primer group and the detection method disclosed by the invention can realize rapid detection of three bacteria on a molecular level, have the advantages of simplicity and convenience in operation, high detection efficiency and accurate result, and are suitable for rapid screening and monitoring of health conditions of experimental animals.

The application discloses an experimental animal behavior monitoring system of a feeding room, and relates to the technical field of animal behavior research.The system comprises a behavior information collection module, which uses a monitoring device to collect original data of experimental animal behavior information of the feeding room in real time, and transmits the original data to a data processing and classification module after collection; the data processing and classification module receives the original data transmitted by the behavior information collection module, calculates, classifies, counts and stores the original data by using a processing and classification algorithm, and forms animal behavior characteristic data after processing the original data, and respectively transmits the animal behavior characteristic data to a correlation analysis module and a comparative analysis module; the data processing and classification module, the correlation analysis module and the comparative analysis module are adopted, original data of experimental animal behavior information collected by the behavior information collection module in real time and comprehensively is accurately processed, and the correlation law and the behavior mode between experimental animal behaviors in a large amount of animal behavior data are found.

The invention discloses a dynamically adjustable animal surgery experiment operation table, belongs to the technical field of animal experiment instruments, and aims at solving the problems that the position adjusting range of a fixing shaft of an existing operation table is limited, and tension control of a traction line depends on artificial experience. According to the technical scheme, fixing shaft sliding grooves are formed in the left side and the right side of a platen, and sliding holes are formed in the bottoms of the grooves for fixing shafts to slide; a translation fixing button is arranged at the bottom of the fixing shaft and is locked and fixed after sliding in place; the top of the fixed shaft is coaxially provided with a traction line length adjusting shaft, and the traction line length adjusting shaft is driven by a take-up button to rotate so as to take up and pay off the wound traction line; the tail end of the traction line binds and locks an animal body through a line locking device and a guide wheel; the side wall of the fixed shaft is provided with a height adjusting button to drive the fixed shaft to stretch. The operating platform realizes multi-dimensional accurate adjustment of animal body positions and stable control of traction tension, and is mainly used for safely and efficiently fixing the body positions of experimental animals in animal surgery experiments.

The invention discloses application of dikafen phenolic acid and ester derivatives thereof in preparation of medicines for treating pulmonary fibrosis. On an MRC-5 cell model induced by TGF-beta1, the bikafen phenolic acid and the multiple ester derivatives thereof show the activity of inhibiting alpha-SMA and COL1A1 protein expression; on a bleomycin-induced pulmonary fibrosis mouse model, the dimethyl ester derivative of the dikafen phenolic acid shows a good protective effect on experimental animals. Therefore, the dicafen phenolic acid and the ester derivative thereof have good application in the field of preparation of medicines for treating pulmonary fibrosis diseases.

The application discloses a method for creating a new strain of Carassius auratus gibelii with resistance to Cyprinid herpesvirus 2 (CyHV2) infection, and the method is characterized in that a gene editing technology is used to target knockout gsdf-a a gene and gsdf-b a gene, and an experimental animal infection model of a homozygous knockout strain of the gene obtained by the technology is established. gsdf Results of the experimental animal infection model show that gsdf the CyHV2 infection resistance of the CyHV2 gene knockout Carassius auratus gibelii is significantly enhanced, the histopathological damage of the Carassius auratus gibelii after being infected with the virus is reduced, the expression of a virus protein ORF47 in liver tissue of the Carassius auratus gibelii is reduced, and the transcription level of a virus gene orf46r is significantly reduced. The new strain of Carassius auratus gibelii created by the method can avoid exogenous gene pollution, and has the advantages that the CyHV2 infection resistance of the Carassius auratus gibelii is significantly enhanced.

This invention relates to the application of β-sitosterol glycoside in the preparation of products for treating lung cancer. This invention creatively provides a new use for the active monomer β-sitosterol glycoside, demonstrating that β-sitosterol glycoside can significantly inhibit the migration ability of lung cancer cells at doses without cell proliferation inhibitors, clarifying the specificity of its anti-metastatic effect, and laying the foundation for further new drug development of this monomer component. Through an in vivo mouse lung metastasis model, this invention demonstrates that β-sitosterol glycoside effectively inhibits the formation of lung metastases while exhibiting no significant toxic side effects in experimental animals, demonstrating high safety, and providing a new candidate molecule for the development of low-toxicity, highly effective anti-lung cancer metastasis drugs.

The utility model provides an experimental animal carrying frame, and belongs to the technical field of animal carrying. Comprising a cage body, a spraying assembly is arranged at the top of the cage body, cage openings are symmetrically formed in the two side walls of the cage body, one side wall of each cage opening is rotationally connected with a cage door, a pair of restraining assemblies are arranged in the cage body, each restraining assembly comprises a screw rotationally connected with the side wall of the cage body, and one end of each screw is rotationally connected with a restraining plate; the spraying assembly comprises a water tank, a water pump is arranged at the bottom in the water tank, and a spraying head communicated with the water pump is arranged at the top in the cage body. Through the spraying assembly, the water pump is started so that an inducer in the water tank can be sprayed into the cage body from the spraying head, experimental animals such as pigs and monkeys with relatively large body types can be induced to enter the cage body, and the distance between the restraining plates can be adjusted through the pair of restraining assemblies according to the body types of the experimental animals; the walking space of the experimental animal in the cage body is effectively reduced, so that the experimental animal is prevented from running back and forth in the cage body or impacting the cage body under assistance, and carrying of the experimental animal is more facilitated.

An apparatus, a system and a method for euthanizing laboratory animals, in particular rodents and lagomorphs, housed in a laboratory cage, the cage having a tub shaped body and an upper rim, wherein the apparatus comprises two sidewalls being arranged parallel to each other, a top panel, a leverage mechanism, and a gas unit for administering a euthanizing gas, wherein the top panel is positioned in between the two sidewalls, and connects the two sidewalls with each other, the top panel comprises at least one gas inlet and one gas outlet, the gas inlet and the gas outlet being connected to the gas unit.

The utility model discloses an animal fixing device for experiments, relates to the technical field of medical experiments, and solves the problem that an animal cannot be turned over quickly and conveniently while being fixed by the conventional animal fixing device. The operation panel and the telescopic frame are arranged in the box body, one end of the operation panel is hinged to the box body through a pin shaft, the operation panel is provided with a protrusion, the box body is provided with a groove, the protrusion is matched with the groove, the operation panel is provided with a through groove, the telescopic frame is connected with the through groove in a sliding mode, and the telescopic frame is connected with the through groove in a sliding mode. According to the utility model, the experimental part of the experimental animal can be simply and quickly changed, the operation of personnel is convenient, and the efficiency can be improved.

The utility model discloses an experimental animal breeding protective fence convenient to install, which comprises side fences, the two side fences are oppositely arranged, door fences are arranged at the ends of the two sides of each side fence through connecting clamps, a spacing fence or a door fence is arranged between the door fences on the two sides through connecting clamps, the side fences are divided into at least two areas, and the side fences are connected with the door fences through connecting clamps. The device is used for feeding experimental animals. According to the detachable fence, every two adjacent fences are connected through the corresponding connecting clamp, and the detachable fence can be detached by rotating the corresponding clamping screw rods, so that the detachable fence is very convenient; during installation, connecting clamps can be installed on the upper side and the lower side at the same time, and the fences are tightly clamped, cannot rotate and are very stable.

A laboratory animal transfer box is provided with a box body, universal wheels are installed at the bottom of the box body, an opening is formed in the upper portion of the box body, a box cover is hinged to the opening, a cleaning opening and a first ventilation window are formed in the front portion of the box body, the first ventilation window is located above the cleaning opening, a second ventilation window is formed in the rear portion of the box body, and limiting plates are horizontally arranged on the inner walls of the left side and the right side in an inner cavity of the box body respectively. The inner cavity is respectively communicated with the first ventilation window and the second ventilation window; a lifting frame is installed in the inner cavity. Good ventilation in the transfer box is guaranteed through the ventilation windows, the fresh air environment needed by the experimental animals can be maintained, and it is avoided that the health of the animals is affected by dirty air in the box. The mesh plate is arranged in the transfer box, so that the excrement and the urine of the experimental animal can fall into the recycling hopper below through the mesh plate, and then are discharged into the collecting box through the blow-off pipe. In the process, effective separation and collection of excrement and urine are realized, pollution to the environment in the box is reduced, and cleanliness of an animal living area is guaranteed.

The present invention discloses an antibody-drug conjugate and a preparation method and application thereof, in particular, a conjugate of an anti-PD-L1 antibody and a TLR7 and / or TLR8 agonist and a pharmaceutical composition, preparation method and application thereof. The present invention obtains a modified anti-PD-L1 antibody with a mutant cysteine ​​by gene editing, which basically retains the structure of the original antibody and can be used for the construction of an antibody-drug conjugate. Through anti-tumor experiments, it was found that the obtained antibody-drug conjugate has better activity, such as strong anti-tumor activity, can significantly improve the survival rate of tumor-bearing animals, and has significantly reduced toxicity, less burden on the body of experimental animals, greatly reduces the minimum effective dose of small molecule drugs when used alone, expands its therapeutic window, and is expected to be used in the development of therapeutic drugs for various diseases (such as tumors, viral diseases such as hepatitis B, etc.), with good application prospects and value.

This invention belongs to the field of animal model technology, specifically disclosing a method for constructing a primary ciliary motor disorder hydrocephalus model, comprising the following steps: screening benzimidazole as a modeling agent; administering benzimidazole solution to experimental animals and setting up a blank control group; conducting experimental verification through pathological morphology and behavioral methods; if the experimental animals show significant ventricular enlargement and significant decrease in motor ability, the primary ciliary motor disorder hydrocephalus model is successfully constructed. This invention employs the above-mentioned novel method for constructing a primary ciliary motor disorder hydrocephalus model. The constructed hydrocephalus model exhibits good stability and repeatability, is objective and simple, and is highly operable, reducing other possible interfering factors. It provides a necessary experimental foundation for studying the mechanism of primary ciliary motor disorder hydrocephalus and developing treatment methods.

This invention discloses a method for preparing HVC NPs for the diagnosis and treatment of Parkinson's disease and their applications. The probe HV in the HVC NPs responds to H2O2 and viscosity, utilizing near-infrared fluorescence imaging capabilities to achieve real-time monitoring of Parkinson's disease biomarkers, helping us better understand the occurrence and development of Parkinson's disease. With the assistance of RVG29, the HVC NPs effectively cross the blood-brain barrier. The curcumin within them can reduce cell apoptosis, lower oxidative stress levels, scavenge excess reactive oxygen species, combat inflammation, and reduce α-synuclein aggregation, demonstrating effective anti-Parkinson's disease effects at the cellular and experimental animal levels. This nanomaterial provides a new strategy for integrated research on the diagnosis and treatment of Parkinson's disease.

1. Name of the product in this design: Multi-channel driveable array electrode. 2. Purpose of this design: For long-term implantation and collection of electroencephalogram (EEG) signals from experimental animals. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The utility model relates to a water feeding structure for experimental animal feeding, which comprises a water inlet pipe, a water outlet pipe and a water outlet pipe, the drainage assembly is installed at the bottom of the water inlet pipe, and the drainage assembly is used for guiding water of the water inlet pipe into the cage. According to the water feeding structure for experimental animal feeding, the drainage assembly is arranged, water of the water inlet pipe can be guided into the drainage groove of the drainage block, opening and closing of the drainage groove are achieved through the locking mechanism, the hidden drinking design can avoid the problem that a water source is exposed in the air for a long time and is polluted and deteriorated, and the service life of the water feeding structure is prolonged. The problem that bacteria breed in the cage due to damp when the water basin is overturned can be avoided, and the water drinking requirements of a plurality of laboratory mice can be met at the same time.

This invention discloses a mobile IVC-type rodent euthanasia device, belonging to the field of laboratory animal euthanasia technology. This device aims to solve the problems of existing euthanasia devices being inconvenient to move, difficult to process multiple cages of animals simultaneously, and wasteful of gas. It includes a chassis with a frame fixed to the top. Multiple cages are bolted to one side of the frame. An air inlet pipe and an air outlet pipe are fixed to the other side of the frame. The air inlet pipe connects to the cages via air inlet branch pipes and an air inlet pipe, and the air outlet pipe connects to the cages via air outlet branch pipes and an air outlet pipe. Both the air inlet and outlet pipes are equipped with a second solenoid valve. An air supply unit is located on one side of the chassis. Rollers are located at the bottom of the chassis, and a handle is located on one side. The device achieves flexible movement through the rollers and handle, and selective ventilation of one or more cages is achieved through the cooperation of a controller and solenoid valves, for the euthanasia of rodent laboratory animals.