388 results about "Experimental animal" patented technology

The invention discloses a humanized osteoporosis mouse model and a construction method thereof, and belongs to the technical field of biotechnology and animal models. The invention aims at the core defects of large species difference, insufficient humanization degree, low pathological simulation degree, unstable phenotype and the like of the existing osteoporosis model. The humanized osteoporosis mouse model is constructed through five core steps of experimental animal pretreatment, collagen-nano hydroxyapatite bionic scaffold preparation and cell pre-implantation, immune-bone metabolism double-derived system construction, progressive osteoporosis induction and model identification and verification. According to the method, double-person origination of immune and bone metabolism systems is realized for the first time, the colonization rate and survival time of human cells are greatly improved, the pathological process of clinical postmenopausal osteoporosis is accurately simulated, the phenotype of the model is stable and uniform, the reactivity matching degree with clinical drugs is high, and the method can be widely applied to research and development of anti-osteoporosis drugs and research of pathological mechanisms.

The utility model discloses a rearing type double-layer experimental animal isolator, and aims to solve the problems that a traditional single-layer rearing isolator is small in animal breeding quantity, large in occupied area and high in labor intensity due to manual excrement removal. The isolator adopts a double-layer box body structure and comprises an upper-layer box body and a lower-layer box body which are communicated with each other, and excrement receiving devices and air conditioning systems are arranged at the bottoms of the isolator. The excrement receiving device is composed of an excrement receiving disc and an excrement scraping device, and excrement is automatically cleaned to the excrement collecting box through an excrement scraping plate. A feces discharge device is arranged in the feces collection box, and a stirring paddle and an electric valve are matched with a concentric-square-shaped bent pipe, so that feces can be discharged in time, and manual intervention is reduced. The air conditioning system adopts an independent air supply system and an air exhaust system, an air supply outlet and an air exhaust outlet are optimally designed to ensure uniform airflow, the air supply outlet and the air exhaust outlet are diagonally arranged, an upper-supply and lower-return air supply mode is adopted, the air supply outlet adopts a static pressure pore plate to supply air, and the air conditioning system has the characteristics of uniform air supply, low noise and small blowing feeling to animals, and provides a good raising environment for the animals.

The invention provides a Garlu virus attenuated strain and application thereof, and relates to the field of virology and biological medicine. According to the invention, the screened Garlu virus attenuated strain is a natural attenuated strain derived from a tick sample, can grow in Vero cells, has the titer of more than or equal to 107 TCID50 / mL, does not have clinical pathogenicity to an immunodeficient host, and is safe; after the attenuated strain is used as an immunogen for pre-intervention, infection of a lethal virus strain can be avoided, the survival rate is increased to 100%, adverse effects on growth and life of experimental animals are avoided, particularly, a host can be stimulated to generate humoral immunity and cellular immunity at the same time, and the survival rate of the experimental animals is improved. And moreover, broad-spectrum cross-protection efficacy can be generated on Bandavirus lethal virus strains, and a foundation is laid for improvement of research on Bandavirus live vaccines such as the Colu virus and the like.

The application discloses an experimental animal behavior monitoring system of a feeding room, and relates to the technical field of animal behavior research.The system comprises a behavior information collection module, which uses a monitoring device to collect original data of experimental animal behavior information of the feeding room in real time, and transmits the original data to a data processing and classification module after collection; the data processing and classification module receives the original data transmitted by the behavior information collection module, calculates, classifies, counts and stores the original data by using a processing and classification algorithm, and forms animal behavior characteristic data after processing the original data, and respectively transmits the animal behavior characteristic data to a correlation analysis module and a comparative analysis module; the data processing and classification module, the correlation analysis module and the comparative analysis module are adopted, original data of experimental animal behavior information collected by the behavior information collection module in real time and comprehensively is accurately processed, and the correlation law and the behavior mode between experimental animal behaviors in a large amount of animal behavior data are found.

The invention discloses application of dikafen phenolic acid and ester derivatives thereof in preparation of medicines for treating pulmonary fibrosis. On an MRC-5 cell model induced by TGF-beta1, the bikafen phenolic acid and the multiple ester derivatives thereof show the activity of inhibiting alpha-SMA and COL1A1 protein expression; on a bleomycin-induced pulmonary fibrosis mouse model, the dimethyl ester derivative of the dikafen phenolic acid shows a good protective effect on experimental animals. Therefore, the dicafen phenolic acid and the ester derivative thereof have good application in the field of preparation of medicines for treating pulmonary fibrosis diseases.

The application discloses a method for creating a new strain of Carassius auratus gibelii with resistance to Cyprinid herpesvirus 2 (CyHV2) infection, and the method is characterized in that a gene editing technology is used to target knockout gsdf-a a gene and gsdf-b a gene, and an experimental animal infection model of a homozygous knockout strain of the gene obtained by the technology is established. gsdf Results of the experimental animal infection model show that gsdf the CyHV2 infection resistance of the CyHV2 gene knockout Carassius auratus gibelii is significantly enhanced, the histopathological damage of the Carassius auratus gibelii after being infected with the virus is reduced, the expression of a virus protein ORF47 in liver tissue of the Carassius auratus gibelii is reduced, and the transcription level of a virus gene orf46r is significantly reduced. The new strain of Carassius auratus gibelii created by the method can avoid exogenous gene pollution, and has the advantages that the CyHV2 infection resistance of the Carassius auratus gibelii is significantly enhanced.

This invention relates to the application of β-sitosterol glycoside in the preparation of products for treating lung cancer. This invention creatively provides a new use for the active monomer β-sitosterol glycoside, demonstrating that β-sitosterol glycoside can significantly inhibit the migration ability of lung cancer cells at doses without cell proliferation inhibitors, clarifying the specificity of its anti-metastatic effect, and laying the foundation for further new drug development of this monomer component. Through an in vivo mouse lung metastasis model, this invention demonstrates that β-sitosterol glycoside effectively inhibits the formation of lung metastases while exhibiting no significant toxic side effects in experimental animals, demonstrating high safety, and providing a new candidate molecule for the development of low-toxicity, highly effective anti-lung cancer metastasis drugs.

The utility model provides an experimental animal carrying frame, and belongs to the technical field of animal carrying. Comprising a cage body, a spraying assembly is arranged at the top of the cage body, cage openings are symmetrically formed in the two side walls of the cage body, one side wall of each cage opening is rotationally connected with a cage door, a pair of restraining assemblies are arranged in the cage body, each restraining assembly comprises a screw rotationally connected with the side wall of the cage body, and one end of each screw is rotationally connected with a restraining plate; the spraying assembly comprises a water tank, a water pump is arranged at the bottom in the water tank, and a spraying head communicated with the water pump is arranged at the top in the cage body. Through the spraying assembly, the water pump is started so that an inducer in the water tank can be sprayed into the cage body from the spraying head, experimental animals such as pigs and monkeys with relatively large body types can be induced to enter the cage body, and the distance between the restraining plates can be adjusted through the pair of restraining assemblies according to the body types of the experimental animals; the walking space of the experimental animal in the cage body is effectively reduced, so that the experimental animal is prevented from running back and forth in the cage body or impacting the cage body under assistance, and carrying of the experimental animal is more facilitated.

The utility model discloses an animal fixing device for experiments, relates to the technical field of medical experiments, and solves the problem that an animal cannot be turned over quickly and conveniently while being fixed by the conventional animal fixing device. The operation panel and the telescopic frame are arranged in the box body, one end of the operation panel is hinged to the box body through a pin shaft, the operation panel is provided with a protrusion, the box body is provided with a groove, the protrusion is matched with the groove, the operation panel is provided with a through groove, the telescopic frame is connected with the through groove in a sliding mode, and the telescopic frame is connected with the through groove in a sliding mode. According to the utility model, the experimental part of the experimental animal can be simply and quickly changed, the operation of personnel is convenient, and the efficiency can be improved.

The utility model discloses an experimental animal breeding protective fence convenient to install, which comprises side fences, the two side fences are oppositely arranged, door fences are arranged at the ends of the two sides of each side fence through connecting clamps, a spacing fence or a door fence is arranged between the door fences on the two sides through connecting clamps, the side fences are divided into at least two areas, and the side fences are connected with the door fences through connecting clamps. The device is used for feeding experimental animals. According to the detachable fence, every two adjacent fences are connected through the corresponding connecting clamp, and the detachable fence can be detached by rotating the corresponding clamping screw rods, so that the detachable fence is very convenient; during installation, connecting clamps can be installed on the upper side and the lower side at the same time, and the fences are tightly clamped, cannot rotate and are very stable.

This invention belongs to the field of animal model technology, specifically disclosing a method for constructing a primary ciliary motor disorder hydrocephalus model, comprising the following steps: screening benzimidazole as a modeling agent; administering benzimidazole solution to experimental animals and setting up a blank control group; conducting experimental verification through pathological morphology and behavioral methods; if the experimental animals show significant ventricular enlargement and significant decrease in motor ability, the primary ciliary motor disorder hydrocephalus model is successfully constructed. This invention employs the above-mentioned novel method for constructing a primary ciliary motor disorder hydrocephalus model. The constructed hydrocephalus model exhibits good stability and repeatability, is objective and simple, and is highly operable, reducing other possible interfering factors. It provides a necessary experimental foundation for studying the mechanism of primary ciliary motor disorder hydrocephalus and developing treatment methods.

This invention discloses a method for preparing HVC NPs for the diagnosis and treatment of Parkinson's disease and their applications. The probe HV in the HVC NPs responds to H2O2 and viscosity, utilizing near-infrared fluorescence imaging capabilities to achieve real-time monitoring of Parkinson's disease biomarkers, helping us better understand the occurrence and development of Parkinson's disease. With the assistance of RVG29, the HVC NPs effectively cross the blood-brain barrier. The curcumin within them can reduce cell apoptosis, lower oxidative stress levels, scavenge excess reactive oxygen species, combat inflammation, and reduce α-synuclein aggregation, demonstrating effective anti-Parkinson's disease effects at the cellular and experimental animal levels. This nanomaterial provides a new strategy for integrated research on the diagnosis and treatment of Parkinson's disease.

1. Name of the product in this design: Multi-channel driveable array electrode. 2. Purpose of this design: For long-term implantation and collection of electroencephalogram (EEG) signals from experimental animals. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The utility model relates to a water feeding structure for experimental animal feeding, which comprises a water inlet pipe, a water outlet pipe and a water outlet pipe, the drainage assembly is installed at the bottom of the water inlet pipe, and the drainage assembly is used for guiding water of the water inlet pipe into the cage. According to the water feeding structure for experimental animal feeding, the drainage assembly is arranged, water of the water inlet pipe can be guided into the drainage groove of the drainage block, opening and closing of the drainage groove are achieved through the locking mechanism, the hidden drinking design can avoid the problem that a water source is exposed in the air for a long time and is polluted and deteriorated, and the service life of the water feeding structure is prolonged. The problem that bacteria breed in the cage due to damp when the water basin is overturned can be avoided, and the water drinking requirements of a plurality of laboratory mice can be met at the same time.

This invention discloses a mobile IVC-type rodent euthanasia device, belonging to the field of laboratory animal euthanasia technology. This device aims to solve the problems of existing euthanasia devices being inconvenient to move, difficult to process multiple cages of animals simultaneously, and wasteful of gas. It includes a chassis with a frame fixed to the top. Multiple cages are bolted to one side of the frame. An air inlet pipe and an air outlet pipe are fixed to the other side of the frame. The air inlet pipe connects to the cages via air inlet branch pipes and an air inlet pipe, and the air outlet pipe connects to the cages via air outlet branch pipes and an air outlet pipe. Both the air inlet and outlet pipes are equipped with a second solenoid valve. An air supply unit is located on one side of the chassis. Rollers are located at the bottom of the chassis, and a handle is located on one side. The device achieves flexible movement through the rollers and handle, and selective ventilation of one or more cages is achieved through the cooperation of a controller and solenoid valves, for the euthanasia of rodent laboratory animals.

The utility model particularly relates to the technical field of experimental animal feeding, in particular to an animal isolation high-oxygen box. The oxygen production box comprises a bottom plate, two first side plates, two second side plates and an oxygen production box body are arranged above the bottom plate, and the bottom plate, the two first side plates, the two second side plates and the oxygen production box body form a closed isolated feeding space; a partition plate is arranged in the isolated feeding space, one side of the partition plate is a high-oxygen suckling mouse area, and the other side of the partition plate is a common-oxygen female mouse area; an operation window is arranged on the first side plate, and a rotating door is arranged at the operation window; filter plates used for filtering excrement are arranged in the areas on the two sides through the transverse inserting grooves, and collecting basins are arranged below the filter plates; according to the device, suckling rats and female rats are bred in different oxygen concentration areas in an isolated mode, while it is guaranteed that the suckling rats and the female rats are correctly paired, damage to the female rats is effectively reduced, the filter plate and the collecting basin at the bottom can improve the sanitary cleanliness of the breeding environment, and the disease risk of bred animals is reduced.

This utility model belongs to the field of laboratory animal technology and discloses a highly flexible self-propelled electric lifting vehicle suitable for SPF barrier environments. It includes a frame with a sealed cavity inside, a modular integrated electrical box within the cavity, a quick-release structure at the opening of the cavity, a lifting assembly on the frame, a liftable worktable on the lifting assembly, a waterproof structure on the worktable, and a walking drive assembly at the bottom of the frame. The quick-release structure includes a quick-release movable plate and multiple sets of bolts. The walking drive assembly includes a first drive wheel, a second drive wheel, and two sets of driven omnidirectional wheels. This utility model has a compact and reasonable structural design, enabling lifting and driving functions. It is suitable for assisting in the rearing of laboratory animals in high-rise buildings. It is small in size, highly mobile, and easy to move between rearing cages. The main circuit components are integrated, making it less susceptible to contamination. It has good waterproof and dustproof performance, is easy to maintain, and convenient to use.

The invention discloses a method for measuring the pressure in the heart cavity of a mouse, and relates to the technical field of experimental animal medical detection, and the method comprises the following steps: cutting an incision of about 1cm in the neck of the mouse; placing two sutures below the common carotid artery, and ligating the common carotid artery by using the suture at the distal end; the other section of suture line is placed in the middle of the right common carotid artery, the carotid artery is lifted up, the blood filling condition is observed, an opening is formed in the blood vessel, a pressure volume catheter is inserted, and meanwhile the suture line at the telecentric end is slightly pulled backwards to prevent bleeding; the pressure volume catheter is inserted into the left ventricle through the right common carotid artery, and the left ventricle pressure maximum rising rate and the left ventricle pressure maximum falling rate are collected. By means of the carotid artery approach, serious trauma caused by thoracotomy can be prevented, and the cardiac physiology can be possibly affected by the operation. Therefore, the carotid artery approach is more suitable for a long-time pressure collection experiment.

The embodiment of the invention provides a signal acquisition wire harness control system and a signal acquisition wire harness control method for an experimental animal. The signal acquisition wire harness control system comprises a visual imaging processing device which is used for acquiring dynamic visual record data of an experimental animal in real time and determining position information of the experimental animal in a world coordinate system and angle information corresponding to the orientation; the control wire harness device comprises a first driving unit used for controlling a signal acquisition wire harness to slide along a first direction; the second driving unit is used for controlling the signal acquisition wire harness to slide along a second direction; the third driving unit is used for controlling the signal acquisition wire harness to rotate; the first driving unit and the second driving unit control the signal acquisition wire harness to move along with the experimental animal in the horizontal plane limited by the first direction and the second direction according to the respectively received position information, and the third driving unit adjusts the signal acquisition wire harness to rotate according to the received angle information; and the signal acquisition wire harness rotates along with the direction of the experimental animal.

The invention relates to a capsule type oxygen cabin for animal experiments, which adopts a modular structure, comprehensively considers the influence of multiple factors such as temperature, humidity, pressure and oxygen concentration, and also considers the influence of breathing oxygen consumption of experimental animals and the influence of a first / second accommodating part, and on the basis, the first / second accommodating part is centrally controlled through a touch screen; according to the method, the combination of multiple modes can be realized, so that the climate conditions of different regions can be accurately, efficiently and stably simulated, and accurate and scientific guidance can be provided for the research on'high reflection ', the work and life of personnel in high and cold climate regions and the pathology research of hospitals. Besides, the electrical parts are classified and placed in the first accommodating part, the second accommodating part, the touch screen and the bearing part, so that the cabin body can be transparent and visual, and the electrical parts in the first accommodating part and the second accommodating part are convenient to overhaul through detachable connection between the first partition plate and the second partition plate and the first accommodating part and between the first partition plate and the second partition plate and the second accommodating part.

The invention discloses a pair of electric handheld homogenate scissors for shearing experimental animal tissues, which is characterized by comprising a shell, a scissor head, a scissor handle, a shearing moving assembly and a driving assembly, the shearing end of the shell is provided with a shear opening used for allowing the shear head to penetrate through. The scissor heads penetrate through the scissor openings; the scissor heads are detachably connected with the scissor handles, and the two scissor handles are arranged in a crossed mode. The shearing moving assembly comprises a moving rod, a moving block, a spring and a guide rod; the guide rod is arranged in the shell, and the movable block is arranged outside the guide rod in a sleeving mode and is in sliding connection with the guide rod. A moving rod is hinged to one end of the moving block, and the other end of the moving rod is connected with the scissor handle; the other end of the moving block is connected with the spring; the other end of the spring is connected with the shell; the driving assembly comprises a motor and a half-tooth gear; teeth are arranged on the side face of the movable block. The scissors are convenient to operate, the scissor heads are convenient to replace, and cross contamination is avoided.

The application discloses an animal tracking and posture estimation method and device, electronic equipment and a storage medium, the method comprising: acquiring an animal ethology video and preprocessing to obtain a plurality of original sampling images; the images are sequentially input into a pre-trained instance segmentation model and a posture estimation model respectively to obtain instance segmentation masks and posture data of each frame of image, and then the original trajectory is created based on the correlation of the instance segmentation masks of adjacent frames; the instance segmentation masks of each frame of image are superimposed into the corresponding original image, and cutting and rotating are performed to obtain instance cutting images; the original trajectory is updated based on the instance cutting images, a preset first trajectory mutual exclusion condition and a preset trajectory matching condition to obtain an animal trajectory; the animal trajectory is combined with the corresponding posture data to generate a visual animal behavior space-time graph. The application solves the problem that current multi-experimental animal tracking and posture estimation cannot be better performed.

The application discloses an animal model and a detection method for evaluating the effects of a substance on hair growth and / or coloring. The evaluation steps are as follows: the experimental animal is plucked, and then the substance to be evaluated is injected into the tentacle pad or the mustache pad or the hair follicle cavity on one side of the experimental animal, and a control solution is injected into the tentacle pad or the mustache pad or the opposite symmetrical hair follicle cavity on the other side of the experimental animal; after a certain number of injections, the hair on the side injected with the control solution grows, while the hair on the side injected with the solution containing the substance to be evaluated grows or does not grow; the length and / or coloring of the grown hair are quantitatively detected and compared, so that the effects of the substance on hair growth and / or coloring can be accurately evaluated. The application utilizes the symmetry of the hair of the experimental animal, and a simple, direct and high-sensitivity method for quantitatively evaluating the effects of a substance on hair growth in vivo is obtained, which can be used for the research on skin diseases and the development of related drugs, cosmetics and the like, and has important scientific significance and application value.

The utility model relates to the technical field of experimental animal storage, and discloses a physiology experimental animal storage device which comprises a storage box. The lifting handle is fixedly arranged on the top surface of the storage box; a door plate and an observation mechanism are sequentially arranged on the front face of the storage box from top to bottom. A transparent glass plate is arranged through an observation mechanism, the state of the experimental animal in the storage box can be observed, the health degree of the experimental animal can be better guaranteed, the self weight of the experimental animal and the elastic potential energy of a spring are utilized to extrude a feeding plate, and then a C-shaped guide plate and a bottom plate are stably limited; the experimental animal storage device is simple in structure and convenient to use, the whole device is relatively stable during movement, meanwhile, after experimental animals are taken out, a mounting frame and a bottom plate can be further rapidly opened by lifting a base plate and releasing clamping of a feeding plate in the vertical direction, rapid cleaning and re-feeding of the storage device are facilitated, a door plate is arranged on the upper portion of the storage box, and the storage device is convenient to use. Experimental animals can be prevented from easily running out of the storage box.

The invention discloses an experimental animal intracerebral target accurate injection system and method based on artificial intelligence and ultrasonic guidance, and relates to the technical field of target injection, the system comprises an ultrasonic probe for emitting ultrasonic waves, receiving experimental animal craniocerebral echo signals and outputting radio frequency signal data; the image processing module is used for executing time gain compensation and beam forming on the radio frequency signal data and outputting gray scale image data and blood flow image data; the artificial intelligence reasoning module is used for inputting the gray-scale image data and the blood flow image data into a convolutional neural network model and outputting corresponding coordinates; the path planning unit is used for generating an entry point coordinate, an angle parameter and a depth parameter through an optimization algorithm; the puncture guide frame adjusts the spatial position of the puncture needle according to the angle parameter and the depth parameter; and the needle point tracking unit is used for calculating a deviation value between the needle point coordinate and a preset track and generating an early warning signal. The problem of inaccurate positioning caused by individual difference in a traditional method is fundamentally solved.

This invention discloses a novel intelligent monitoring device for an experimental island used for object recognition, belonging to the field of experimental animal behavioral monitoring technology. It includes an environmental control module housed within the experimental island enclosure to regulate temperature, humidity, light intensity, and odor levels, maintaining environmental stability. A behavior acquisition module, also housed within the island, collects data on the experimental animals' exploratory behavior, physiological correlations, and object interactions during the experiment. A data processing module, communicatively connected to the behavior acquisition module, receives these data, performs fusion analysis based on a preset algorithm, calculates a recognition index, and generates a multi-dimensional experimental report. This invention, from environmental control and stimulus presentation to data acquisition and analysis, minimizes human error and subjective bias, ensuring the reproducibility and cross-laboratory comparability of experimental results.

The invention provides an experimental animal sign information acquisition device capable of preventing data counterfeiting. The experimental animal sign information acquisition device comprises an information acquisition module, an experimental target placement module and an information processing module, the information acquisition module comprises an image acquisition part and a rotating part; the rotating part drives the image acquisition part to rotate by taking an experimental target as a center, so as to realize 360-degree acquisition of image data of the experimental target; the experimental target placement module comprises a tray and a pressure sensor; the pressure sensor is used for weighing an experimental target; and the information processing module collects information of the image acquisition part and the pressure sensor, feeds back the information and displays the information through a display screen of the information processing module. According to the technical scheme, various kinds of sign information of the experimental animal can be collected at the same time.

The application discloses a mouse and Mongolian gerbil universal immunization sampling fixing device, and relates to the field of experimental animal fixing devices.The device comprises a supporting base, a bearing support is arranged on the front end surface of the supporting base, a sampling fixing plate is rotatably arranged on the outer surface of the bearing support, guide sliding grooves are symmetrically arranged on the upper surface of the sampling fixing plate, movable blocks are movably arranged in the guide sliding grooves, and a limiting sleeve is fixedly arranged on the front surface of the sampling fixing plate and extends from one end of the movable blocks.The synchronous transmission structure of the supporting disc, the first connecting plate and the second connecting plate drives the movable blocks on the two sides to synchronously move towards each other or in the opposite direction along the guide sliding grooves, and the movable blocks are matched with the positioning support plates which can slide in the limiting sleeve, so that the horizontal spacing and the longitudinal extension length of the limb fixing points can be simultaneously adjusted, the adjustment range completely covers the size difference of adult mice and adult Mongolian gerbils, and two special fixing devices are not needed, so that the universality of the device is improved compared with the prior art.

The utility model relates to the technical field of storage boxes, in particular to an experimental animal tissue storage box. The storage box comprises a storage box body and a reagent shelf, a box cover is installed on the upper surface of the storage box body, adjusting structures are arranged on the two sides of the inner wall of the storage box body, each adjusting structure comprises two fixing plates, the two fixing plates are fixedly connected with the storage box body, sliding grooves are formed in the two sides of each fixing plate, and the fixing plates are fixedly connected with the storage box body. A sliding groove is formed in one side of the fixing plate, a sliding block is slidably connected to the inner wall of the sliding groove, a connecting groove is formed in one side of the fixing plate, a clamping plate is slidably connected to the inner wall of the connecting groove, the clamping plate is fixedly connected with the sliding block, a spring is fixedly connected to the inner wall of the sliding groove, the spring is fixedly connected with the sliding block, and positioning frames are fixedly connected to the two sides of the reagent shelf. And the positioning frame is slidably connected with the clamping plate, the inner wall of the sliding groove is fixedly connected with a limiting rod, and the limiting rod is slidably connected with the sliding block. The problem that reagent bottles of different models cannot be placed on the reagent shelf is solved.