1593 results about "Experimental animal" patented technology

Disclosed is a cage for breeding laboratory animals. The laboratory animal breeding cage can detachably couple a lid to a body of the cage in a simple manner. Further, by virtue of a double filter mounted in the lid, the laboratory animals can be bred under both positive and negative pressure. Furthermore, by virtue of a double safety valve, the inside and the outside of the cage can be doubly air-blocked. The cage can be conveniently washed an managed, the cause of failure is eliminated and a life span of the cage is lengthened, and the efficiency in breeding and controlling the laboratory animals are enhanced.

Provided are methods for determining concurrently with a simple, minimally invasive test, the adequacy of pancreatic beta-cell compensation and / or the presence of tissue insulin resistance in a subject human or an experimental animal. The methods allow for the determination of a subject's or experimental animal's susceptibility to developing type 2 diabetes mellitus (DM2) or to progression to more advanced forms of DM2. Among other uses, the methods allow for diagnostic classification of subjects for decisions regarding therapeutic interventions, clinical differentiation between type 1 DM and DM2, clinical monitoring of treatments intended to reduce risk of developing DM2 in non-diabetic subjects, clinical monitoring of agents intended to improve existing DM2 and to prevent progression of DM2, clinical development and testing of new compounds, candidate agents, or candidate therapies for preventing progression to DM2 or disease progression in existing DM2, and preclinical screening of candidate agents or candidate therapies in experimental animals to identify and characterize agents having insulin-sensitizing properties, pancreatic stimulatory or regenerative properties or other desirable actions.

The invention relates to an animal model for obese rats and an establishing method, and belongs to the field of animal genetic engineering and genetic modification. The animal model for obese rats and the establishing method are characterized in that rats with LEP and LEPR genes knocked out are obtained through the CRISPR / Cas9 technology. The rats with LEP and LEPR genes knocked out have a typical obesity characteristic, the reliable animal model can be provided for studying the obesity disease of people, and the rats with LEP and LEPR genes knocked out are expected to become a standard experiment animal of obese rats.

The invention discloses a visual cognitive behavioral learning automatic training system of big and small mice. The visual cognitive behavioral learning automatic training system of the big and small mice comprises a training box, a main control unit, a video monitor, a microcontroller, a reward unit, a punishment unit, and a visual clue display unit. The microcontroller is connected with the main control unit through a universal serial bus (USB) interface in a communication mode. The training box is a structure that an opening is formed in the upper portion of the training box. The video monitor is arranged on the upper portion of the training box. The reward unit is composed of a food plate which is arranged on the lower portion of the training box, an infrared induction switch which is arranged on one side of the training box, and a water supply pipe which is arranged above the food plate and controlled by an electric control valve. The punishment unit is composed of a sweep frequency ultrasonic speaker which is arranged on the upper portion of the training box. The visual clue display unit comprises a capacitance-type touch screen and a display screen. The capacitance-type touch screen is arranged inside a frame. The lower frame of the frame is hinged with the side wall of the training box. The upper frame of the frame is connected with the side wall of the training box through a tension spring. The display screen is arranged at a rear position of the capacitance-type touch screen. The visual cognitive behavioral learning automatic training system of the big and small mice has the advantages that a visual cognitive behavioral learning automatic training to experimental animals is achieved.

A method for measuring hemodynamics of an experimental animal enabling repeated measurements, the method comprising the following steps: (i) ligating at least two parts of an artery, between which a part to be incised is located, of the experimental animal, the parts comprising at least one proximal ligated part and at least one distal ligated part, and then incising a part between the ligated parts; (ii) inserting through the incised part a blood pressure-volume simultaneously measuring catheter provided with multiple conductance electrodes and at least one pressure sensor; (iii) loosening the distal ligated part so as to allow an insertion of the catheter and not to cause bleeding in the opened part; (iv) further inserting the catheter so as to introduce the multiple conductance electrodes and the pressure sensor(s) into the heart ventricle; and (v) simultaneously measuring pressure and volume of the heart.

The invention relates to the technical field of medical experimental facilities, and discloses a small animal brain three-dimensional positioning system for magnetic resonance imaging scanning equipment. The system comprises a magnetic compatibility small animal brain three-dimensional positioning device used for conducting brain three-dimensional positioning on the head of a small animal and a magnetic resonance small animal radio frequency coil device which is used for receiving a magnetic resonance radio frequency signal in the X axis direction and transmitting the signal to a magnetic resonance imaging system. According to the system, in the piercing implementation process, magnetic resonance scanning can be carried out on an experimental animal, the magnetic resonance radio frequency signal is received through the small animal radio frequency coil, the signal is transmitted to the magnetic resonance imaging system, a magnetic resonance scanning image can be utilized for guiding and monitoring the piercing process, the magnetic resonance imaging scanning equipment can be utilized for guiding a small animal three-dimensional positioning operation or conducting positioning experiments such as piercing and stimulation exertion.

The invention provides an application of a type of berbamine derivatives and salts thereof in the preparation of drugs for the treatment of tumors, which is mainly applied in the preparation of the drugs for the prevention and treatment of nuclear transcription factor NF-kBp65 activity-related diseases and BCR / ABL transcription activity-related diseases. The drugs are combined and prepared by the compounds of the invention and one or more pharmaceutically acceptable excipients. The preparation forms comprise solid preparations, semi-solid preparations or liquid preparations. The type of berbamine derivatives and the salts thereof provided by the invention have broader and stronger anti-leukemia and anti-solid-tumor activity, the tumors proved to be sensitive are leukemia, multiple myeloma, liver cancer, osteosarcoma and breast cancer; the toxicity and the side effects are lighter. An in vitro cell culture system and animal experiments confirm that the berbamine derivatives and the salts thereof have no significant toxicity or side effects to the growth of normal human hematopoietic cells and experimental animals under the anti-tumor dosage, which are superior to the commonly used chemotherapy drugs.

The invention provides a device for causing damage to an experimental animal, which comprises a platform and an altitude controller arranged on the platform, and further comprises a striking component, a horizontal bracket and a vertical bracket; the striking component comprises a striking bar, a striking head, a guiding pipe, an electromagnet, a power and a switch; the guiding pipe, which is an open-ended through-tube, is fixed on the horizontal bracket; a cover of the striking bar is arranged in clearance fit with the guiding pipe; one end of the striking bar is connected with the striking head, and the other end of the striking bar is made of ferromagnetic materials and arranged in the magnetic field produced by the electromagnet; the electromagnet and the power are electrically connected with the switch; the altitude controller and the vertical bracket are vertically connected with the horizontal bracket; and the electromagnet is fixed on the vertical bracket. The device causes fixed-point and fixed-amount damage to animal tissue such as spinal cord, reduces artificial errors through mechanized operation, and enhances the repeatability of an experiment.

The invention discloses a multi-function animal weight-losing running table and a control system thereof. The running table comprises a running machine, a weight-losing support device and a recovery robot. Running training or combination training of running training and electric stimulus is performed on the experimental animal by arranging organic glass and a stimulating electrode on the running machine; and the weight-losing running table training combining running table training and weight-losing support is performed on the experimental animal by arranging the weight-losing support device on the running machine. In the process of weight-losing running table training, the recovery robot can assist hind legs of the experimental animal in doing exercise training, so that a correct or preset training task is provided for a passive training model, the movement locus of the hind legs in the positive training model is recorded and the record is used for quantitatively evaluating the exercising function. The invention also provides a medicinal animal experiment device which is mainly used for walking function recovery of the experimental animal with hurt spinal cord and providing the animal running table training needed by sports medicine.

The invention provides a preparation method of a freeze-dried powder standard sample of an animal-sourced substrate drug and a metabolite residue thereof. The method includes following steps: feeding experimental animals with drugs, wherein a drug feeding method is feeding, injecting or medicated bath with one or more drugs; controlling a feeding time; obtain a sample material containing raw drugs and / or metabolites through metabolism in animal bodies; adding a vitamin C solution; performing a mashing and homogenizing process to obtain meat paste; performing a vacuum freeze-drying process, a crushing and sieving process and a nitrogen-filling package process and a vacuum packaging process. In addition, a degreasing, repeated homogenizing and sieving process is included or not included according to fat content in an animal substrate. The standard sample can be transported at normal temperature and is long in preserving time. A situation of combination of a target object and the substrate is coincided with that of an actual detection sample. The standard sample can be used of quality controlling of relative detection and analysis, is homogeneous and stable, is convenient to transport, is reliable in use and has significant economic value and market competitiveness.

An animal memory training system with a closed rotary maze comprises a maze device and a stimulating device. A rotary wheel (6) driven by a motor (4) is provided on the bottom of the case (1) of the maze device. The motor (4) is set in a central column, and its output shaft is tightly connected with the rotary wheel (6) along the central column shaft direction, thus the rotary wheel (6) is driven by the motor (4) to rotate around said central column. A number of pulleys (12) are set on the lower periphery of the frame of the case (1) to support said rotary wheel (6), and a sieve-like supporting net (5) is arranged above the rotary wheel (6). The stimulating device includes an automatic manipulator stimulating device and a remote control stimulating device. Compared with the existing maze model, the rotary drive action of the training system's rotary wheel is moderate, and is more coincident with the movement actual of animals' normal physiological behavior. Further, the stimulating device can respectively realize the rewards and punishments for the experimental animals, and has the characteristics of accurate positioning, timely stimulation, complete stimulation variety, no ravage and convenient operation. The training system has overtaken the existing shortcomings of unilateral dominance in the detection of the present maze training systems, such as Y-maze and T-maze detection.

The invention discloses an automatic device for evaluating the memory function of a primate experimental animal and a method for evaluating the memory function of the primate experimental animal. The device comprises an experimental operation cage (1), a touch display screen (15), a horizontal exhibition stand (12), an infrared ray sensing automatic award food distributor (16) and a main control computer (19). Through interactive operation of the touch display screen and the animal, direct interactive operation between experiment personnel and the animal is avoided, and the evaluation of the cognitive ability is more objective. Through the device, the brain function can be evaluated subjectively according to a judgment made by a tester. The device and the method are particularly suitable for evaluating the cognitive memory of primate animals, particularly old non-human primate experimental animals and disease model non-human primate experimental animals.

The invention relates to a reconstructed ovum of an albinism model pig and a construction method thereof, and a construction method of the model pig. According to the construction method of the reconstructed ovum, tyrosinase is knocked out to obtain the reconstructed ovum of the albinism model pig. The combination of a CRISPP / Cas9 gene knock-out technique and a somatic nucleus transplantation technique proves that the method for constructing the gene modified pig has high feasibility. The obtained TYR gene knock-out pig has the typical characteristics of albinism, can provide a reliable animal model for researching human albinism, can be applied to toxicity and anaphylaxis detection and many other aspects, and is hopeful to become a standardized experimental animal for albinism-like mice.

The invention discloses a preparation method of anti-fluoroquinolone rabbit monoclonal antibodies and application thereof. Six fluoroquinolone medicaments are chosen in the invention and coupled with Bovine serum albumin (BSA) to synthesize immunoantigens respectively. The above six immunoantigens are injected subcutaneously in the backs of immune rabbits after being mixed together. The anti-fluoroquinolone rabbit monoclonal antibodies are obtained by hybridoma technique. Rabbit monoclonal antibodies, which are superior to rat monoclonal antibodies, are prepared in the invention. More antigenic determinants are identified when rabbits are chosen as the experimental animal instead of rats. In addition, more integrated experiments can be carried out in rabbit spleens, since the spleens of rabbits are larger than that of rats. The invention provides the possibility of high throughput screening for fusion cells. The anti-fluoroquinolone antibodies of wide-adaptability in the invention has good detection effect on fluoroquinolones, such as enrofloxacin, ofloxacin, Difloxacin, Pefloxacin, Fleroxacin, Danofloxacin, etc., thus making it applicable to fluoroquinolone residue detection on food, forage and environmental samples.

The invention discloses a method and systems for monitoring daily activities of an animal, and belongs to the technical field of image data processing. The method comprises the following steps of: acquiring a background image of an animal breeding box, and putting the animal in the box; acquiring an animal image, subtracting the animal image from the background image, and marking pixel points higher than a threshold during subtracting; calculating average coordinates of the marked pixel points, and calculating movement displacement and movement distance; marking position coordinates which correspond to the movement distance and are less than or equal to a set threshold as stationary points, and marking sleeping once when the number of the continuous stationary points is more than or equal to the threshold of the number of the stationary points; and acquiring sleep rhythms, sleep time, sleep frequency and motion trail of the experimental animal according to the marked result. The invention also provides two kinds of systems for monitoring the daily activities of the animal. The method and the systems can perform non-interfering monitoring on a plurality of experimental animals for a lone time, and automatically acquire and analyze experimental data such as sleep cycle rhythms, time, frequency and motion trail of the experimental animals.

The invention belongs to a construction method of an animal model, and in particular relates to a construction method of a cynomolgus macaque model for alcoholic liver disease. The construction method of the cynomolgus macaque model for alcoholic liver disease comprises the following steps: screening experimental animals, feeding the screened experimental animals with high-glucose and high-fat feed, and conducting liver biopsy and acquisition of the determined model. The construction method of the cynomolgus macaque model for alcoholic liver disease provided by the invention is short in duration and high in success rate; the construction method is suitable for researching the occurrence mechanism of the alcoholic liver disease and is convenient for screening drugs for treating the alcoholic liver disease and for evaluating the efficacy of the drugs on the alcoholic liver disease; and the construction method has a good application prospect.

The invention discloses a water-in-oil-in-water adjuvant vaccine and a preparation method thereof. The adjuvant vaccine is composed of an internal water phase, a middle oil phase used for cladding the internal water phase, and an external water phase for cladding the middle oil phase. The external water phase comprises: a composite surfactant, a hydrophilic surfactant and mormal saline. The middle oil phase consists of: a lipophilic surfactant, a stabilizer and mineral oil. The internal water phase comprises: an immunopotentiator solution, the hydrophilic surfactant, and an inactivated antigen solution. The W / O / W (water-in-oil-in-water) type adjuvant vaccine provided in the invention can induce an immunoreaction earlier than traditional W / O (water-in-oil) type adjuvant vaccines, and can make experimental animals obtain earlier protective antibodies. The vaccine has good absorption, and causes a small inflammatory response at an inoculation site. The adjuvant vaccine disclosed in the invention has better stability than existing W / O / W type vaccines, and the antibody generation speed is fast.

The present invention relates to a ventilated shelving storage system for housing laboratory animals. The ventilated shelving storage system according to the present invention is of the type suitable for being connected to the centralised air circulation system of a building, or of the room where the shelving storage system is located, while at the same time each shelving storage unit is designed to have its own filter unit comprising at least one antiparticulate filter. Another object of the present invention is a system of shelving storage units in series suitable for being connected to a centralised air circulation system and comprising an antiparticulate filter unit.

The invention aims to make it unnecessary to reposition an objective lens when repeatedly carrying out examinations. It is possible to carry out in vivo examination of tissue such as cells and muscle tissue or various internal organs, such as the heart, liver, and so forth, of mammals, especially small experimental animals, over a comparatively long period of time. A microscope system having an objective optical system unit and a structure or member for fixing the objective optical system unit to a living body is provided.

The provides expanded feeds for an experimental dog, which comprise expanded corn flours, corn flours, flours, wheat flours, soybean meal, corn protein powders, bran powders, fish meal, chicken meal, egg yolk powders, trace elements, calcium hydrophosphate, yeast, table salt, fish oil, multivitamin and soybean oil which are mixed together; the expanded feeds for the experimental dog adopts the formula manner of combining vegetable protein with animal protein and carbohydrate, the trace elements necessary for growing are also added in the expanded feeds according to the physiological characteristics of the experimental dog, and the adequate demand of the experimental dog on the nutrition can be satisfied; in addition, the growth rate of the experimental dog is higher compared with conventional feeds and various physiological indices can completely satisfy the experimental animal national standards by adopting the expanded feeds for the experimental dog.

A mobile platform arrangement includes a first (mobile) platform and a second (support) platform. Both are flat-bottomed members, wherein the support platform is preferably, but not exclusively, provided with sidewalls and wherein a diameter of the support platform exceeds an outer diameter of the mobile platform at least twice. The mobile platform is advantageously positioned onto and / or within the support platform. The top surface of the support platform is provided with a friction reducing surface structure. The mobile platform is therefore adjusted to perform gliding motion along and / or over the friction reducing surface, wherein acceleration necessary for performing gliding motion is imposed to the mobile platform by an external force, provided by movement of an experimental animal within the mobile platform. The mobile platform arrangement in addition may include a mechanism for securing a conscious wake and able to move experimental animal, including a head adapter and corresponding mount.

The invention discloses a closed rotary maze device for an animal memory training system. The maze device comprises a round box body and a circuit control system. Key technical points of the maze device are that: the bottom of the box body of the maze device is provided with a turntable driven by a motor; the motor is arranged on a central cylinder, and an output shaft of the motor is in fastenedwith the turntable along the axial direction of the central cylinder; the turntable rotates around the central cylinder under the driving of the motor; the periphery of the lower part of a frame of the box body is provided with a plurality of pulleys for supporting the turntable; and a sieve-shaped supporting net is arranged above the turntable. The maze device overcomes the defect of the lateralization in the detection of the conventional maze devices such as a Y-maze and a T-maze. In addition, a device driven by gradient voltage is removed; the maze device effectively prevents animals from finishing experiments by means of smell, and respective disadvantages of a Morris water maze, the Y-maze and a drought maze are weighted; the starlight detection can be performed through program control according to the nocturnal habits of the experimental animals; and the maze device can ensure that the experimental animals are subjected to sensory stimulation in real environment, which is more favorable for acquisition of experimental data.

The present invention is directed to non-pathogenic, oncolytic, recombinant polioviruses for the treatment of various forms of malignant tumors. The recombinant polioviruses of the invention are those in which the internal ribosomal entry site (IRES) of the wild type poliovirus was exchanged with the IRES of other picornaviruses, and optionally P1, P3 or the 3'NTR thereof was exchanged with that of poliovirus Sabin type. More particularly, the present invention is directed to the administration of the non-pathogenic, oncolytic, recombinant poliovirus to the tumor directly, intrathecally or intravenously to cause tumor necrosis. The method of the present invention is particularly useful for the treatment of malignant tumors in various organs, such as: breast, colon, bronchial passage, epithelial lining of the gastrointestinal, upper respiratory and genito-urinary tracts, liver, prostate and the brain. Astounding remissions in experimental animals have been demonstrated for the treatment of malignant glioblastoma multiforme, an almost universally fatal neoplasm of the central nervous system.

The invention discloses a method for detecting diarrhea shellfish toxin, which comprises the following steps: A) establishing a standard curve of depolymerization of okadaic acid (OA) induced HL-7702 hepatocyte F-actin; B) carrying out the calculation on the concentration of the OA according to the standard curve of the F-actin depolymerization of the HL-7702 hepatocyte, establishing a detection method of the hepatocyte F-actin of the diarrhea shellfish toxin to determine the possible detection limit range; C) selecting one or more of components (STX, DA and YTX) which are possibly coexist with the diarrhea shellfish to act on the HL-7702 hepatocyte, determining the specificity of the method for detecting the OA content by detecting the degree of damaging the polymerizing power of the HL-7702 hepatocyte F-actin by the above components. The detection method of the invention has the advantages: (1) taking cells as experimental subjects to avoid the using of experimental animals and conforming to the rule of 3R; (2) having high sensitivity; (3) having good specificity; (4) having good repeatability; and (5) having simple and convenient sample extraction and low matrix effects.

The invention discloses pediococcus pentosaceus and application thereof. The Latin name of the pediococcus pentosaceus is Pediococcus pentosaceus LI05, and the pediococcus pentosaceus is preserved in the China General Microbiological Culture Collection Center and has a preservation number of CGMCC No.7049. The pediococcus pentosaceus disclosed by the invention has the morphological characteristics that the thallus is rod-shaped, dose not generate spores, does not have motility, and has masculine Gram staining. The whole-cell fatty acid of the pediococcus pentosaceus disclosed by the invention comprises the following main components in percentage by weight: about 1.45% of 14:0, about 3.26% of 16:1w7c / 16:1w6c, about 31.25% of 16:0, about 6.31% of 18:1w9c, about 38.59% of 18:1w7c, about 1.37% of 18:0 and about 14.59% of un18.846 / 19:1w6c. The invention also discloses a complete sequence of 16S ribosome DNA (16SrDNA) of the pediococcus pentosaceus. The pediococcus pentosaceus and a preparation thereof disclosed by the invention can be used for adjusting the micro-ecological balance of intestinal tracts of human or animals, promoting digestive absorption and slowing down the happening and development of liver failure of experimental animals.

The invention provides a controllable microenvironment flowing gas animal experiment cabin which comprises a closed experiment cabin body, wherein an experimental animal and a temperature, humidity, pressure and oxygen concentration sensor group are placed and arranged on an air-permeable platform positioned in the closed experiment cabin body; the upper part of the cabin body is connected with a nitrogen gas source, an oxygen gas source and a compressed air source and controls gas proportion through a control valve; the compressed air source is provided with a temperature-reducing dehumidifier and an air supply filter; the experiment cabin body is provided with a humidifier and a heater; a gas inside the experiment cabin body is discharged through a flow regulating valve and a negative-pressure exhaust fan; a computer simulates different environments, such as a high-temperature environment, a low-temperature environment, a high-pressure environment, a low-pressure environment, a oxygen-rich environment, a low-oxygen environment, and the like by controlling inlet gas flows, gas concentration proportion and the parameters, such as temperature, humidity, oxygen concentration and the like, of an environment inside the experiment cabin body through a data line according to a preprogram to carry out a flowing gas animal experiment; and the parameters, such as an experiment process, a detection result, and the like, are stored or printed into a laboratory report. The controllable microenvironment flowing gas animal experiment cabin provided by the invention is widely applied to the scientific research fields of environment, medicine, military, avigation, and the like.

A system for providing an interface for interacting with a laboratory animal and the method of using the system are disclosed.

The invention discloses an animal operation and experiment table. The animal operation and experiment table comprises a table top of an experiment table, rails and an operation table, wherein the table top of the experiment table is provided with a first imaging hole which passes through the table top of the experiment table; the rails are arranged on the table top of the experiment table so as to fix an imaging device; the operation table is movably arranged on the table top of the experiment table and is provided with a second imaging hole which passes through the operation table; and the first image hole and the second imaging hole are kept coaxial. According to the animal operation and experiment table, the operation table is combined with the imaging device into a whole, the blood flow can be continuously detected in real time before, in or after the process of operation on animals, and the requirements of imaging blood vessels and the blood flow of the experimental animals at different body positions (such as lying on the back or lying on stomach) can be met.

The present invention discloses an experimental animal low-pressure cabin for plateau medicine research, and a pressure control method based on the experimental animal low-pressure cabin, and belongs to the technical field of medical scientific research instrument equipment. The experimental animal low-pressure cabin comprises a work cabinet and an electrical cabinet arranged on the bottom portion of the work cabinet, wherein the work cabinet adopts the full transparent design so as to easily meet the illumination requirements of the experimental animals and make the laboratory personnel easily perform real time observation on the experimental animals, a gas inlet pipe extending into the work cabinet and a gas discharge pipe extending into the work cabinet are arranged inside the electrical cabin, the gas inlet pipe and the gas discharge pipe are respectively provided with an electrically controlled proportional valve and a diaphragm vacuum pump, an electrical program control system is further arranged inside the electrical cabinet, and a PLC controller adjusts the dynamic balance between the gas complementing amount and the gas exhaust amount by controlling the opening degree of the electrically controlled proportional valve and the opening of the diaphragm vacuum pump so as to make the altitude fluctuation be maintained at a certain level. According to the present invention, the air pressure control way used by the control method of the present invention is unique, the fluctuation range is small, the accuracy is high, and the number of the gas exchange can be ensured.

This invention provides a method to monitor a microorganism that causes infectious disease of a laboratory animal by using a micro flow channel chip immobilized with a molecular to be tested such as an antigen or an antibody of the microorganism that causes infectious disease, the method comprises flowing serum or body fluid taken from the laboratory animal through the minute flow channel of the micro flow channel chip and detecting the antigen antibody reaction on the chip. The method of this invention enabled medical inspection of an infectious disease of a laboratory animal and microorganism monitoring of a laboratory animal, by using minute amount of animal serum or body fluid in a closed system rapidly and sensitively.