Preparation method of freeze-dried powder standard sample of animal-sourced substrate drug and metabolite residue thereof

Abstract

The invention provides a preparation method of a freeze-dried powder standard sample of an animal-sourced substrate drug and a metabolite residue thereof. The method includes following steps: feeding experimental animals with drugs, wherein a drug feeding method is feeding, injecting or medicated bath with one or more drugs; controlling a feeding time; obtain a sample material containing raw drugs and/or metabolites through metabolism in animal bodies; adding a vitamin C solution; performing a mashing and homogenizing process to obtain meat paste; performing a vacuum freeze-drying process, a crushing and sieving process and a nitrogen-filling package process and a vacuum packaging process. In addition, a degreasing, repeated homogenizing and sieving process is included or not included according to fat content in an animal substrate. The standard sample can be transported at normal temperature and is long in preserving time. A situation of combination of a target object and the substrate is coincided with that of an actual detection sample. The standard sample can be used of quality controlling of relative detection and analysis, is homogeneous and stable, is convenient to transport, is reliable in use and has significant economic value and market competitiveness.

Description

technical field [0001] The invention belongs to the technical field of animal source matrix standard samples for detection of drug residues, specifically a method for raising animals with common veterinary drugs to obtain animal matrix with a considerable drug concentration, and vacuum freeze-drying to form freeze-dried powder standard samples. Background technique [0002] Typical veterinary drugs refer to drugs used to prevent and treat livestock and poultry diseases. However, with the development of intensive breeding production, some chemical and biological medicinal ingredients have been developed into animal health products or feed additives with certain functions, which also belong to the category of veterinary drugs. The main uses of veterinary drugs are to prevent and treat diseases, promote growth, improve production performance, and improve the quality of animal food. Veterinary drug residues refer to the accumulation and storage of the original compounds of vete...

AI Technical Summary

Problems solved by technology

At present, domestic residue analysis laboratories often cannot obtain actual contaminated samples, so they often add veterinary drug standard solutions to the analysis samples for quality control, which cannot truly reproduce the extraction efficiency of drugs in animals after natural metabolism, and cannot reflect the method. Accuracy, the test results obtained are not convincing enough

The European Union’s regulations on veterinary drug residue analysis methods and result expression 2002 / 657 / EC clearly pointed out the need to use standard samples for process control. Foreign residue analysis laboratories have generally used standard sample samples for laboratory quality control and result evaluation, mainly by Provided by the Institute of Standard Materials and Measurement (IRMM) of the European Community and FAPAS of the United Kingdom, relevant samples have also been industrialized, but due to the high price of foreign standard samples or quality control samples, and the need for transportation Problem, domestic residue analysis laboratories cannot regularly use it for quality control

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