Patents
Literature
Patsnap Copilot is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Patsnap Copilot

648 results about "Veterinary drug" patented technology

Veterinary prescription drugs are those drugs restricted by federal law to use by or on the order of a licensed veterinarian [Section 503 (f) Food, Drug, and Cosmetic Act]. The law requires that the drug sponsor label such drugs with the statement: "Caution: Federal law restricts this drug to use by or on the order of a licensed veterinarian."

Fluorescent micro-ball immune chromatography test paper strip for detecting residual animal medicine and preparing method thereof

The invention discloses a test paper strip for the fluorescent bead immunochromatography of veterinary medicine remained in a detected sample and a preparation method thereof. A filter paper, a sample pad, a fiberglass membrane, a pyroxylin membrane and water sucking paper are sequentially stuck on a base plate in related joint; fluorescent beads are sprayed on the pyroxylin membrane for marking a veterinary medicine resistant molecule monoantibody; the pyroxylin membrane coated with a veterinary medicine molecule holoantigen is used as a detection region; the pyroxylin membrane coated with an anti-mouse antibody is used as a quality control region; and the test paper strip is prepared through the following steps: (1) the preparation of the pyroxylin membrane; (2) the preparation of a fluorescent bead pad; and (3) the assembling of the test paper strip. In the detecting process, emitted spectrums pass through a proper optical filter device; and all the emitted spectrums are collected through CCD scanning technology, are congregated, are multiplied and are analyzed through a fluorescence analyzer and relevant software to obtain a quantized fluorescence signal, thereby realizing quantitative detection. The test paper strip is mainly used for the qualitative detection and the quantitative detection of all the veterinary medicine classes in food safety detection.
Owner:WUXI ZODOLABS BIOTECH

Method for simultaneously detecting multi-kind pesticide residues in bee products

InactiveCN101358953ASolve the problem of matrix effectFast wayComponent separationRetention timePhosphate
The present invention relates to a method of simultaneously detecting a plurality of agro-veterinary drug residues in bee products. The extracted liquid trichloroacetic acid or perchloric acid and the extracted liquid acetate, phosphate or borate solution are added into a sample; the pH value is controlled between 4.5 and 9.0; the mixed solution is centrifuged, the filtrate is added into a solid phase extraction column to be extracted, the extraction column is eluted and dried, the column is washed by oxalic acid-methanol solution, the volume of the eluent is defined by the aqueous solution of methanol, the eluent is added into liquid chromatography-tandem mass spectrometry to be analyzed and tested, the acquired chromatographic peak is contrasted with the known standard chromatographic peak of the drug, and according to the retention time and the abundance of the mass spectrum ions, the specific name of the detected drug is determined. The method only requires one pre-treatment of the sample, and thus can simultaneously extract 11 classes and more than 60 kinds of veterinary drug residues, such as sulfonamides, quinolones, macrolides, lincomycins, nitroimidazoles, beta-lactams, tetracyclines, chloromycetins, trinethoprims, chlordimeform, triadimenol and the like, the efficiency of analysis is high, and the detection cost is greatly reduced.
Owner:中华人民共和国江苏出入境检验检疫局

Method of detecting residue of small-molecule substance harmful to human body and a special kit

The invention discloses a method of detecting the residue of small-molecule substances harmful to the human body and a special kit. The special kit comprises a non-transparent micro-porous plate and a light-emitting compound, wherein each hole of the non-transparent micro-porous plate is filled with a coat antigen which is simultaneously coated with three kinds of small-molecule substances. The invention makes full use of the multi-color marking function of QDs, establishes a novel kit for simple and rapid detection of the residue and a method thereof, and realizes the multi-color marking through indirect marking of polyclonal antibodies and monoclonal antibodies in the veterinary drug by coupling the QDs with different particle sizes and targets with functional groups (such as an amino) with specific surfaces. The method comprises: obtaining quantum dots with different fluorescent characteristics through separation and purification, namely, multi-color antibody markers, using the multi-color antibody markers as fluorescent probes, and establishing a reaction system for synchronous analysis of various antigen components of different kinds, thereby realizing the synchronous detection of multiple kinds of residues of the veterinary drug in animal food. Moreover, the method has the advantages of simple operation, high fluorescence intensity and long stabilization time.
Owner:CHINA AGRI UNIV

Preparation method and application of molecular imprinting electroluminescent sensor for detecting trace veterinary drug residue by taking battery as power

The invention discloses a molecular imprinting electroluminescent sensor for detecting veterinary drug residue by taking a battery as power and a method for detecting veterinary drug residue. A preparation method of an electrode (a schematic diagram is shown in the figure) comprises the following steps of: preparing MIP (Molecularly Imprinted Polymer)s sol of the veterinary drug residue; preparing a carbon dot and preparing a graphene nano material according to documents; and modifying graphene, the carbon dot and the MIPs sol onto the surface of the electrode of the sensor. The method for detecting the trace veterinary drug residue comprises the following steps of: connecting the modified electrode to an electrogenerated chemiluminescence instrument, and detecting the veterinary drug residue in a sample extract by taking the battery as the power. The molecular imprinting electroluminescent sensor disclosed by the invention has the advantages that the specificity of the electrode is strong and the sensitivity is high and can be up to a ng grade; only 3-5 minutes are spent on a basic detection process; and the cost is low. The method for detecting the veterinary drug residue by adopting the electrode is quick and easy to operate, the reaction is automatically completed by instruments and results are automatically recorded by the instruments.
Owner:UNIV OF JINAN

UPLC-MS/MS simultaneous flux detection method for multiclass veterinary drug residue in raw fresh milk

The invention discloses a UPLC-MS / MS simultaneous flux detection method for multiclass veterinary drug residue in raw fresh milk. The method includes the steps of: (1) extracting a veterinary drug compound in a to-be-detected sample to obtain an extracted solution; (2) concentrating the extracted solution, and then regulating the pH to 7.5-10.0 to obtain a column passing solution; (3) activating a solid phase extraction column; and (4) loading the column passing solution on the activated solid phase extraction column, leaching the solid phase extraction column with leacheate, then conducting elution with eluent, collecting the eluent, carrying out concentration, redissolving and filtration by a filter membrane, then performing qualitative and quantitative determination by UPLC-MS / MS. The method provided by the invention can simultaneously detect 38 veterinary drug residue in raw fresh milk, and has the characteristics of high sensitivity and low detection limit. The matrix standard curve correlation degree, the standard addition recovery rate of the method and the intra-day and inter-day precision are all accord with the China, European Union and international veterinary drug residue analysis method requirements. The method provided by the invention provides efficient and accurate detection means for raw fresh milk veterinary drug residue risk analysis and warning research.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI +1

Detection method for simultaneously measuring residue of tetracyclines (TCs) drugs in royal jelly

The invention relates to a detection method for simultaneously measuring the residue of tetracyclines (TCs) drugs in royal jelly, in particular to a detection method for simultaneously measuring the residue of 10 veterinary drugs including oxytetracycline, tetracycline, demethylchlortetracycline, chlorotetracycline, doxycycline, 4- epioxytetracycline, 4-epitetracycline, 4-epichlorotetracycline, minocycline and methacycline. The method comprises the following steps: precipitating protein by methanol; then, adjusting the pH value; further carrying out multi-stage purification; and simply and simultaneously measuring the residue of the TCs drugs by the liquid chromatography-mass spectrometry/mass spectrometer (LC-MS/MS). By carrying out the pre-treatment for one time, the invention can measure the residue of up to 10 TCs drugs in the royal jelly, wherein, the lower limits of detection (LLD) of the five TC antibiotics including oxytetracycline, tetracycline, demethylchlortetracycline, chlorotetracycline and doxycycline are 2.0 mug/kg; while the LLDs of the other veterinary drugs including 4- epioxytetracycline, 4-epitetracycline, 4-epichlorotetracycline, minocycline and methacycline are 10.0 mug/kg.
Owner:THE INSPECTION & QUARANTINE TECH CENT ZHEJIANG ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Preparation method of enramycin premix

The invention relates to a preparation method of an enramycin premix, comprising the following steps of: carrying out high-pressure filtration/squeeze on enramycin fermentation liquor prepared by using a conventional method; separating the fermentation liquor; then carrying flash drying on an obtained enramycin filter cake and then crushing; adding a powdery carrier to fine mycelium powder according to the purity and the end-product requirement content of a dried product so as to regulate the concentration of the fine mycelium powder; evenly mixing to obtain a powdery enramycin premix; and pelleting the powdery enramycin premix into a granulated enramycin premix according to demands. The preparation process of the preparation method does not need to refine and extract enramycin, does not consume organic solvents and acid and base, and has less pollution on the environment, and the production cost is largely reduced; the content and the uniformity of the obtained product meet the quality standard requirements of imported veterinary drugs, and the stability of the product meets the specified requirements; moreover, dangerous solid wastes, such as antibiotic bacterium residues and the like, are not generated in the production and preparation process, thereby greatly reducing the pollution to the environment and the later-period management cost.
Owner:濮阳泓天威药业有限公司

Preparation method for liquid chromatography mass spectrometry (LC-MS)-grade high-purity acetonitrile reagents

The invention relates to a preparation method for liquid chromatography mass spectrometry (LC-MS)-grade high-purity acetonitrile reagents. The method includes steps: subjecting raw acetonitrile to adsorption by the aid of adsorbents such as activated carbon and activated alumina, oxidizing for purification, rectifying, dehydrating and drying by the aid of a molecular sieve, filtering by the aid of a microfiltration membrane, filling nitrogen for packaging, and obtaining the LC-MS-grade high-purity acetonitrile reagents. By the method, the recovery rate is more than 98%, the yield is more than 95%, the purity reaches to more than 99.99%, application requirements of clients on scientific researches and subject studies of the LC-MS-grade high-purity acetonitrile reagents can be met, domestic vacancies of production of the LC-MS-grade high-purity acetonitrile reagents can be filled, dependency on foreign reagents is lowered, high-quality reagents are provided for growing wide application of LC-MS quantitative analysis in high-tech fields such as biomedical research and development, clinic, food safety detection, pesticide residue analysis, veterinary drug residues, legal examiners, criminal investigations, doping detection, drug testing, agriculture and environment protection.
Owner:天津康科德医药化工有限公司

Simultaneous screening and detection method of plurality of types of veterinary drug residues in solid animal-derived foods

The invention relates to a simultaneous screening and detection method of a plurality of types of veterinary drug residues in solid animal-derived foods. The simultaneous screening and detection method comprises the following steps: after taking and crushing a sample to be detected, adding an acetonitrile acetate solution and a Na2EDTA buffering solution into the sample to be detected; meanwhile, adding anhydrous sodium sulfate and sodium chloride, and homogenizing and extracting; adding an adsorbent containing C18 and NH2 into extracting-obtained liquid supernatant to carry out purification; then determining by using high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. The screening method provided by the invention can be used for simultaneously screening and detecting the plurality of types of veterinary drug residues in the animal-derived foods, has the characteristics of rapidness, simplicity and convenience, flexibility, accuracy and the like, can be used for carrying out an accurate qualitative analysis on target veterinary drugs in the sample, and also can be used for carrying out quantitative detection. The method is applicable to rapid detection of the plurality of types of veterinary drug residues in batch samples. Aiming at the solid animal-derived foods, the impact on the target veterinary drugs by sample substrates is reduced, and the extraction effect is good and the recycling rate is high.
Owner:中华人民共和国临沂出入境检验检疫局

Detection method of residual quantities of various veterinary drugs in culturing or slaughtering environment

The invention provides a detection method of residual quantities of various veterinary drugs in a culturing or a slaughtering environment. With the method, rapid screening and quantitative detection can be carried out upon 61 drugs of 9 categories. The drugs include chlormycetin, beta lactams (penicillins), quinolones, sulfonamides, trimethoprims, macrolides, tetracyclines, and nitroimidazoles. According to the invention, a soil sample or an environmental water sample is added into a phosphate buffering solution; a filtrate obtained through centrifugation is extracted in a solid phase extraction column, and is eluted; the extraction column is dried by blowing, and is washed by using a methanol solution; an obtained eluent is titrated by using a methanol solution; a chromatogram peak of the sample is detected by using liquid chromatography-tandem mass spectrometry; the chromatogram peak is compared with a standard chromatogram peak, such that a specific variety of the detected drug is accurately determined. According to the invention, the sample solution is subject to liquid chromatography-tandem mass spectrometry multi-reaction monitoring selected ion analysis. Through internal standard correction, the recovering rate is 70-120%, and a relative standard deviation RSD is no larger than 18%. Compared with existing technologies, the analysis efficiency is improved by at least 5 times, and the detection cost is 30% of that of existing technologies.
Owner:ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU

Animal source food sulfonamide residual medium solid phase dispersion-highly effective liquid phase chromatography determination method

The present invention relates to 'method for examining solid-phase dispersion -high performance liquid chromatogram of residues substrate of sulfonamides in animal derived food' which belongs to the field of food safety-veterinary drug residues. The method comprises of chromatogram examining steps of substrate solid-phase dispersion and high performance liquid, wherein, the mobile phase of sodium dihydrogen phosphate-acetonitrile is 50mmol/L in high performance liquid chromatogram examining method, the volume ratio of sodium dihydrogen phosphate and acetonitrile is 70:30, because the sodium dihydrogen phosphate solution is buffer solution which has a certain PH buffer capability and makes the PH value stable, the stability of pH value of HPLC mobile phase is ensured, and repeatability and reproducibility of analysis result are ensured also, at the same time, the classical process of extraction, separation, concentration and purification are simplified, the operation is simple, the milk combination phenomenon is eliminated. The method of present invention has the merits of ideal precision and sensitivity, and environment protecting by using the less toxicity organic solvent eluting analyte employed.
Owner:BEIJING JINXIU DADI AGRI CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products