Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A high-performance liquid chromatography, sulfonamide drug technology, applied in the field of food safety-veterinary drug residues, can solve the problems of retention time change, consumption of a large amount of solvent, poor heat resistance of sulfonamide drugs, etc., and achieves easy control and stability of pH value. Results that guarantee repeatability and reproducibility, ideal accuracy and sensitivity
Inactive Publication Date: 2008-08-13
BEIJING JINXIU DADI AGRI CO LTD
View PDF0 Cites 16 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0007] 1) In HPLC analysis, acid (formic acid, acetic acid, phosphoric acid, etc.)-organic solvents are often used as mobile phases. Phosphoric acid, formic acid, acetic acid, etc. are volatile, and their concentration or content will change if they are left for a long time, so they are very unstable at different times. It is difficult to prepare the same precise pH value, which affects the repeatability of the analysis results in different analysis time, leads to the change of retention time, and even makes the analytes unable to separate from each other
[0008] 2) MSPD elution uses toxic organic solvent dichloromethane, which is
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0024] Example 1: Detection of fish meat samples
[0025] 1. Main equipment and medicines
[0026] instrument:
[0027] 1) Glass mortar;
[0028] 2) Glass syringe 5mL
[0029] 3) SPE solid phase extraction device
[0030] 4) Nitrogen blower with constant temperature water bath
[0031] 5) Pipette 1mL, 0.5mL
[0032] 6) Volumetric flask 10mL
[0033] 7) Electronic balance (division value 0.001g)
[0034] 8) Timer
[0035] 9) Vortex mixer
[0036] 10) Liquid Chromatograph
[0037] drug:
[0038] 1) Standard material (purity ≥98.5%): sulfadiazine, sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfamonomethoxine ( sulfadoxine), sulfamethoxazole (sulfamethoxazole), sulfaquinoxzline (sulfaquinoxzline), sulfadimethoxine (sulfadimethoxine) standard solution.
[0079] The operation steps are the same as above, and the HPLC test results of pork samples are shown in image 3 .
[0080] Under the analytical conditions established by the present invention, the chromatograms ( image 3 , figure 2 and figure 1 ), it can be seen from the separation effect of these chromatograms that there is no matrix interference to affect the determination of the drug, and the separation effect is very good.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The present invention relates to 'method for examining solid-phase dispersion -high performance liquid chromatogram of residues substrate of sulfonamides in animal derived food' which belongs to the field of food safety-veterinary drug residues. The method comprises of chromatogram examining steps of substrate solid-phase dispersion and high performance liquid, wherein, the mobile phase of sodium dihydrogen phosphate-acetonitrile is 50mmol/L in high performance liquid chromatogram examining method, the volume ratio of sodium dihydrogen phosphate and acetonitrile is 70:30, because the sodium dihydrogen phosphate solution is buffer solution which has a certain PH buffer capability and makes the PH value stable, the stability of pH value of HPLC mobile phase is ensured, and repeatability and reproducibility of analysis result are ensured also, at the same time, the classical process of extraction, separation, concentration and purification are simplified, the operation is simple, the milk combination phenomenon is eliminated. The method of present invention has the merits of ideal precision and sensitivity, and environment protecting by using the less toxicity organic solvent eluting analyte employed.
Description
technical field [0001] The invention belongs to the field of food safety-veterinary drug residues, in particular to a method for detecting sulfonamide drug residues in animal-derived food. Background technique [0002] Sulfonamides (SAs) are a general term for a class of drugs with the structure of p-aminobenzenesulfonamide, which are a class of traditional synthetic antibacterial drugs. It is similar to p-aminobenzoic acid (PABA) in chemical structure. The two compete with dihydrofolate synthase, inhibit the synthesis of bacterial dihydrofolate, and affect the production of bacterial nucleic acid, thereby preventing the growth and reproduction of bacteria. Sulfonamides have a broad antibacterial spectrum and have inhibitory effects on most gram-positive bacteria and some gram-negative bacteria. In animal feeding, sulfonamides are commonly used to treat livestock metritis, respiratory and digestive tract infections, swine atrophic rhinitis, fowl cholera, typhoid, paratyphoi...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more