335results about How to "Guaranteed repeatability" patented technology

The invention aims to provide a method and a device for determining the peak area of chloride ions based on ion chromatography. The method for determining the peak area of chloride ions based on ion chromatography comprises the following steps: step 1, identifying a chromatographic peak: peak separation, peak qualitation, baseline correction, peak trailing treatment and peak overlapping treatment; and step 2, calculating the chromatographic peak area: peak definition, integration algorithm, calibration curve application, repeatability verification, impurity peak deduction and main peak area calculation. According to the method, a chromatographic peak identification and chromatographic peak area calculation mode is designed, so that the technical problem that other impurity ions may be introduced to influence chloride ion chromatographic peak identification and accurate area calculation in an existing mode of determining organic chlorine through ion chromatography is solved.

The invention relates to the technical field of battery testing, and discloses a truck lithium battery testing method and device based on Bluetooth communication. The method comprises the following steps: performing intelligent remote matching processing on Bluetooth identification information of a truck lithium battery management system to obtain a remote Bluetooth control key, and performing lithium battery data acquisition protocol test processing to obtain a dual-channel acquisition instruction set; performing dynamic voltage, current and temperature cooperative acquisition processing on the dual-channel acquisition instruction set to obtain a multi-dimensional battery working condition characteristic spectrum; remote parameter linkage calibration processing is carried out on the multi-dimensional battery working condition characteristic spectrum, an intelligent calibration control sequence is obtained, remote charging and discharging management test processing is carried out, and a battery running state monitoring record is obtained; and performing remote health assessment processing on the battery running state monitoring record to obtain a lithium battery performance test report. According to the lithium battery testing scheme, physical connection is not needed, operation is easy and convenient, and the real-time monitoring capability is achieved, so that the testing efficiency and flexibility are improved.

The invention discloses a serum-free culture system and culture method of NK cells. The invention firstly discloses a serum-free culture system of the NK cells, wherein the serum-free culture system comprises a coating solution, an amplification culture medium, an activation culture medium and autologous plasma; the invention further discloses a culture method for NK cells by using the serum-free culture system. The method for culturing the NK cells by using the serum-free culture system disclosed by the invention reduces the culture cost of the NK cells, can simply, quickly, efficiently and safely obtain a large number of NK cells with high activity, high purity and strong killing ability in vitro, provides a better cell source for clinical application of the NK cells, and promotes further development of an NK cell treatment technology.

The invention discloses a full-automatic intelligent multifunctional high-order nonlinear fluorescence dynamics testing method and device, and relates to the field of nonlinear optics and nano photonics. An optical testing device composed of an exciting light generation module, an automatic power regulation module, an exciting light modulation module, a sample scanning module, a data acquisition and analysis module and an intelligent control module is established, and under the excitation of incident laser, based on the overall coordination of the intelligent control module, the optical testing device is established. Other modules are driven to complete the dynamic response test of a nonlinear fluorescence sample, and full-automatic intelligent, multifunctional, high-precision, simple, efficient, low-cost and universal fluorescence dynamic test analysis is realized. The problems that traditional manual operation wastes time and labor and the testing precision is low are solved. In addition, the multifunctional integration of the fluorescence response curve, the fluorescence rise time, the fluorescence lifetime and the fluorescence imaging effectively improves the test efficiency and the application scene, and greatly reduces the system cost.

The invention provides a double-ring micro-motion monitoring method and system based on a monocular camera, and relates to the technical field of machine vision, and the method comprises the steps: obtaining a picture based on the monocular camera, building a double-ring center point, determining the relation between projection points of the double-ring center point on an image plane and a normalization plane, and obtaining a single-ring micro-motion monitoring result; converting a plane coordinate corresponding to the central point of the double circular rings into a pixel coordinate and determining a conversion relation; and determining the pixel offset of the central point of the double rings under the pixel coordinates, solving the real offset under the real coordinates based on the pixel offset, and determining the action of the double rings. According to the invention, highly automatic object positioning and tracking can be realized, manual intervention is reduced, production efficiency and operation precision are improved, and an accurate object positioning automation application which is high in cost benefit, high in precision and easy to implement is provided.

The invention relates to the technical field of analysis and detection, and particularly provides a method for predicting the transfection efficiency of fluorinated lipid nanoparticles by using multi-nuclear magnetic resonance, and the method comprises the following steps: (1) carrying out nuclear magnetic resonance fluorine spectrum detection on fluorinated nanoparticles to obtain a nuclear magnetic fluorine spectrum of a control group; (2) transfecting the fluorinated nanoparticles into a cell or a mouse living body, and performing nuclear magnetic resonance fluorine spectrum detection on the cell or the living body to obtain a nuclear magnetic fluorine spectrum of an experimental group; and (3) calculating the difference value of the target peak areas of the fluorinated lipid in the experimental group and the control group to predict the transfection efficiency of the fluorinated lipid nanoparticles. The method has good performance in the aspects of specificity, repeatability, stability, linear relation and the like, and can provide effective technical support for large-scale screening of nano medicaments of fluorinated lipid nanoparticles.

The invention discloses a high-brightness piezoelectric mechanical luminescent material with the chemical formula of LiNbO3: 0.01 Pr, xZn and a preparation method and application thereof, x is larger than 0 and smaller than or equal to 0.02, the preparation process is simple, and the prepared material has the mechanical luminescent characteristics of high brightness, long-term storage and repeatability, can be used as a mechanical luminescent material as a stress distribution visualization material, and can be applied to the field of light emitting devices. Or the optical anti-counterfeiting function can be realized as an anti-tamper label.

The invention provides a gas permeability testing device and method, and the device comprises a gas control device which provides gas for a sample and controls the supply of the gas; the heating device is used for heating the sample so as to test the air permeability of the sample at high temperature; the control device is electrically connected with the gas control device and the heating device and is used for controlling gas supply and heating temperature in the gas permeability testing process; wherein a temperature sensor is arranged in the heating device and is used for monitoring the temperature in the heating device in real time and transmitting the temperature in the heating device to the control device so as to correct and calculate the air permeability test result. According to the air permeability testing device, accurate temperature monitoring and adjustment are achieved through the built-in temperature sensor, and the accuracy of air permeability testing results of the sample at different temperatures is ensured.

The invention discloses a newly-hatched silkworm counting method. The method comprises the steps of S1, acquiring a newly-hatched silkworm image; s2, binarizing the newly-hatched silkworm image to obtain a binarized image; s3, counting connected regions in the binarized image, establishing a histogram by taking the area and the corresponding number of the connected regions as coordinates, and determining a standard area according to the area distribution of the connected regions; and S4, determining the number of newly-hatched silkworms contained in all the connected regions according to the standard area to obtain the total number of newly-hatched silkworms. The invention further provides a newly-hatched silkworm counting device. According to the invention, the number of newly-hatched silkworms can be accurately counted, and the statistical efficiency is improved.

The utility model relates to the technical field of in-vitro intestinal tract simulation, in particular to an animal in-vitro intestinal tract analogue simulation device which comprises a main body container, a plurality of intestinal tract simulation units are arranged in the main body container, and a feeding system, a circulating system and a control system are further arranged in the main body container. The feeding system, the circulating system and the control system are electrically connected with the intestinal tract simulation unit. The digestion process in the animal intestinal tract can be simulated more truly through the arrangement of the multiple intestinal tract simulation units, and each unit can independently simulate different digestion stages. Due to the integrated design of the feeding system, the circulating system and the control system, the flowing of food in the intestinal tract, the action of digestive enzymes and the intestinal tract movement can be effectively simulated. By arranging the wriggling control piece and the contraction and relaxation piece, wriggling movement in the intestinal tract can be simulated, and the authenticity of simulation is enhanced.

The invention relates to the field of medicines, and provides a tevirimat composition as well as a preparation method and application thereof. The composition contains tevirimat serving as an active component, cyclodextrin serving as an inclusion agent, meglumine serving as a solubilizer and an alkaline additive in a weight ratio of 1: (15-30): (0.05-0.2): (0.1-0.2). Wherein the solubility of the active ingredient tevirimat can be greatly improved through the matching and combined use of the three auxiliary materials including the inclusion agent, the solubilizer and the alkaline additive. Compared with the prior art, the dosage of each auxiliary material in the composition can meet the specified limit in related laws and regulations, the allergy risk, the hemolysis risk and the stimulation risk are low, and the preparation safety is better. The composition provided by the invention is simple and feasible in preparation method, more stable in preparation finished product quality and more convenient to apply. The composition can be used for treating or preventing virus infection and related diseases caused by orthopoxvirus.

The invention discloses an operation state control method applied to a centrifugal machine. A rotating shaft is driven to rotate according to a rotating speed median value so that mixed liquid can be evenly distributed into each set of rotating drums; after separation of n parts of mixed liquid is completed, a set of rotary drums with the best separation effect in centrifugal separation of each set of rotary drums is confirmed, and the optimal centrifugal force of mixed liquid separation is obtained through calculation; calculating the radius of the energy-saving rotary drum to obtain a corresponding energy-saving rotary drum sleeve, and configuring the optimal rotating speed of the rotating shaft according to the position of the rotary drum sleeve and the optimal centrifugal force; and the mixed liquid is guided to the fixed drum sleeve. And driving the rotating shaft to rotate according to the optimal rotating speed, and separating the mixed liquid. Mixed liquid can be separated at the same time through multiple centrifugal forces. Through one-time separation of a plurality of rotary drum sleeves, the most suitable separation rotary drum can be determined at the fastest speed, and the separation efficiency is improved. And only one set of drum sleeve is used for separation again, so that the efficiency is high, and meanwhile, energy is saved.

The utility model relates to a device for measuring the displacement of an optical compression mold with high precision, which comprises a position detection device, a movable mold seat, a fixed mold seat, a movable mold and a fixed mold, a plurality of support columns are arranged above the movable mold seat, support plates are fixed in front of and behind the movable mold seat, a base plate is arranged above the support plates, and the movable mold seat and the fixed mold are arranged on the base plate. A first groove is formed in the upper surface of the base plate, a belleville spring capable of applying extrusion force to the movable mold is arranged in the first groove, the movable mold is located above the base plate, the fixed mold is located above the movable mold, the fixed mold is fixed to the fixed mold base, and an ejection structure for ejecting a product is arranged on the movable mold base. The position detection device is fixed to the outer side of the movable mold of the mold and used for detecting the compression position of the mold, a reset mechanism for resetting the ejection structure is arranged on the ejection structure, and an ejection rod via hole is formed in the bottom of the movable mold base. According to the utility model, high-precision monitoring of the compression position of the optical compression reflector can be realized.

The invention discloses a plant cell nucleus extraction method and application, and belongs to the technical field of plant single cell sequencing. The method comprises the following steps: carrying out tissue immobilization on plant tissues by using precooled methanol; preparing a cell nucleus lysis solution, namely an NIB solution; cleaning and chopping the fixed tissue sample, and incubating the tissue sample in an NIB solution; filtering with a screen; centrifuging, discarding supernatant, and resuspending and precipitating the NIB solution to obtain a suspension; slowly dripping the suspension into a Percoll solution, and carrying out density gradient centrifugation to purify cell nucleuses. The invention provides a high-applicability and high-quality cell nucleus extraction method which is suitable for cryopreserved or fresh tissue samples, higher cell nucleus yield and mRNA capture rate are obtained, and the data quality of single cell sequencing is improved.

The utility model relates to the technical field of resistance detection, and relates to a heat dissipation type detection jig. According to the utility model, the resistor product is positioned through the positioning groove on the positioning jig, and the moving assembly drives the detection assembly to move towards the resistor product, so that the plurality of probe parts on the detection frame are connected with the resistor product, and the resistance value of the resistor product can be obtained through an external test instrument. The same repeatability of resistance test of the resistor product is ensured, and the test accuracy is ensured; a heat dissipation through hole is formed in the positioning groove, so that when the resistor product is detected by a probe, a space at the bottom of the resistor product is in contact with air, and a heat dissipation effect is achieved; the moving assembly drives the plurality of probe parts to detect the resistor element in a manner of pressing down from the top, so that the probe parts can be exposed in the air, and a heat dissipation effect is achieved.

The invention provides a question and answer system evaluation method and device, electronic equipment and a storage medium, and the method comprises the steps: inputting an evaluation question into a question and answer system, and obtaining an associated text obtained by the question and answer system through retrieval based on the evaluation question, and an output answer generated by the question and answer system based on the evaluation question and the associated text; based on the evaluation question and the associated text, determining the retrieval precision of the question and answer system; and determining an evaluation result of the question and answer system based on the retrieval precision and the output answer. According to the method and device, the electronic equipment and the storage medium provided by the invention, comprehensive evaluation is carried out on the question-answering system from two aspects of retrieval performance and answer generation performance, manual participation is not needed, subjectivity and inconsistency possibly introduced by manual evaluation are eliminated, objectivity, comprehensiveness and repeatability of evaluation of the question-answering system are ensured, and the evaluation efficiency of the question-answering system is improved. Therefore, an accurate and reliable evaluation result is obtained.

The utility model relates to the technical field of adhesion performance test platforms, and discloses a viscose product adhesion performance test platform which comprises an operation table and a connecting block, a lifting box is installed on the rear portion of the upper end of the operation table, a sliding groove is formed in the middle of the front end wall of the lifting box, a driving motor is installed at the inner bottom end of the lifting box, and a test plate is installed at the lower end of the connecting block. The lower end of the tension sensor penetrates through the connecting block and is connected with the test plate, a detection box is installed in the middle of the upper end of the operation table, and a baffle is installed on the right side wall of the operation table; the two motors are started, the motors drive the stirring blades to rotate through the motor shafts, the viscose products are stirred evenly, it is guaranteed that the concentration of the viscose products is even, the heater is started, the viscose products are heated to different temperatures for adhesion performance testing, experimental data are richer and more comprehensive, and the collected data are high in accuracy and small in error.

The invention discloses a core optical image equalization processing method based on a domain adaptive residual error full connection network, comprising the following steps: S1, estimating illumination components based on a Retinex theory and dynamic Gaussian filtering, realizing brightness domain adaptive division according to the difference of the illumination components, and recording boundary position coordinates of each partition; s2, cutting the original color digital rock core image according to the boundary coordinates of each partition recorded in the step S1, and storing the cut original color digital rock core image as an independent image file; s3, constructing a residual error full-connection network combining a transfer learning strategy and a domain adaptation algorithm, wherein a training target is to minimize a domain adaptation loss function to obtain an optimal prediction model; s4, using the optimal prediction model to correct an image edge region and outputting a brightness-equalized core image; according to the method, the brightness equalization effect can be remarkably improved while image details are kept, and the accuracy and generalization ability of the lithology recognition model can be greatly improved.

The invention discloses a primer group and a kit for detecting spinal muscular atrophy related genes and a kit using method, the primer group comprises an amplification primer group and an extension primer group, primer nucleotide sequences in the amplification primer group are as shown in SEQ ID NO: 1-14, and primer nucleotide sequences in the extension primer group are as shown in SEQ ID NO: 15-26. The kit provided by the invention comprises the primer group, a PCR reaction reagent, an SAP reaction reagent and an extension reaction reagent, and can simultaneously detect SMN1 and SMN2 gene copy numbers, 7 hotspot mutations in people and c.* 3 + 80Tgt closely related to silent carriers in one reaction; the qualitative detection of the G site has a wider detection range for spinal muscular atrophy. Meanwhile, the amount of sample templates required by nucleic acid detection of the kit is small, the detection limit of the nucleic acid templates can be as low as 1 ng / mu L, and the kit has good sensitivity, specificity, stability and accuracy and can meet the current needs of screening patients with spinal muscular atrophy and silent carriers and prenatal diagnosis of infants.

The utility model discloses a culture system for a poison attacking experiment of aquaculture, which comprises a plurality of culture ponds for receiving a water source and aquatic products and completing the poison attacking experiment of the aquatic products in the culture ponds; the culture pond comprises an adding group and a control group, pathogenic factors are added into the adding group, and the control group is configured to provide blank control for the adding group; the water treatment device is connected with the culture ponds through a pipeline assembly, and the water treatment device is configured to treat water after the challenge experiment is completed in the culture system so that the water can be recycled; and the control cabinet is configured to control the water treatment device to treat water quality. The device disclosed by the utility model can be well matched with a control experiment and a pathological experiment required by a challenge experiment, is convenient to observe and can be repeatedly used.

This utility model discloses an automotive duct cutting device with a rapid positioning mechanism, relating to the field of duct cutting technology. It includes a worktable and an upper clamp. A groove is formed on the upper side of the worktable. A first motor is fixedly connected to one side of the worktable, and a lead screw is fixedly connected to one side of the output shaft of the first motor. The lead screw is rotatably connected to the inner side of the groove. A slider is threaded onto the outer side of the lead screw, and a cutting mechanism is mounted on the upper side of the slider. A lower clamp is fixedly connected to the upper side of the worktable, and a spring-loaded mechanism is installed between the upper and lower clamps. A cavity is formed inside the worktable, located below the groove, and a positioning mechanism is installed inside the cavity. This device uses a motor-driven connecting rope to quickly pull down the upper clamp to press the duct, combined with precise control of the cutting position by the lead screw, and automatic release using a tension spring. The entire process is coordinated by a processor, achieving fully automatic, efficient, and precise operation of the duct from positioning and cutting to release.

The invention provides a non-contact on-line measuring device and method for excavator structural members, a double-scanning system cooperation mode is adopted, a tracker and a ball cage scanner are respectively used on two sides of a detection device, the ball cage scanners are installed on a cooperative robot to execute automatic scanning actions, and meanwhile, the robot is driven by the robot to move. The tracker and the robot are both installed on the linear guide rail, displacement operation can be carried out, the scanning and collecting range of the system is expanded, and therefore full scanning work of the large structural part is completed. The two scanning systems scan and collect the structural member from the left side and the right side at the same time, then data are spliced together, a three-dimensional point cloud model of the structural member is obtained, size information is analyzed and extracted, a detection report is generated, communication with a workshop data management system is carried out, and measurement data are integrated and applied.

The invention provides an epoxy modified cement-based material, the epoxy modified cement-based material comprises an organic phase structure and inorganic crystals, and the inorganic crystals are radially distributed on the surface of the organic phase structure; the organic phase structure is a spherical or sphere-like structure with the diameter of 3-15 microns; the inorganic crystal is of a needle-shaped, rod-shaped or sheet-shaped structure with the length being 0.3-3 microns and the diameter being 0.05-0.5 microns. The epoxy modified cement-based material disclosed by the invention shows good adhesion and dispersion resistance under water or in a humid environment, can realize rapid underwater construction, and keeps stable performance for a long time under harsh conditions such as a marine environment and a humid environment; the engineering requirements of multiple fields such as building structure repairing, underwater repairing, rapid first-aid repair, ocean protection and road and bridge engineering are met, and the integration of high strength, high toughness, strong interface bonding and long-acting durability is realized.

The invention discloses a stator measuring device, which belongs to the technical field of automobile parts size detection. The invention comprises a stator turntable assembly, a stator rotating assembly, and a displacement sensor measuring assembly. The stator turntable assembly comprises a turntable pad, a stepper motor, a synchronous belt, and a cam divider; the stator rotating assembly comprises a stepper motor, a synchronous belt, and a rubber roller; the displacement sensor measuring assembly comprises a fixed support, a screw rod, a guide rail, a synchronous belt, a servo motor, and a high-precision displacement sensor. The invention provides a stator measuring device which is easy to operate, has high measurement efficiency, is suitable for production line production of stators and online measurement.

The invention belongs to the technical field of hair tail essential oil, and discloses persistently-shaped double-layer hair tail essential oil and a preparation method thereof.The preparation method comprises the following steps that a water-phase component with a shaping function is prepared, and the water-phase component comprises a water-soluble film-forming polymer; preparing an oil phase component with a repairing function, wherein the oil phase component comprises vegetable oil and an oil-soluble coloring agent; and filling: filling the water-phase component and the oil-phase component, and layering the water-phase component and the oil-phase component. The shaping and repairing agent has double synergistic effects of shaping and repairing, the oil-phase component provides deep nourishing, the water-phase component provides lasting shaping force and is used after being shaken up, perfect combination of shaping and repairing is achieved, and the problem that a traditional product is single in function is solved; lasting shaping; the unique visual experience is realized.

The invention provides a parameterization calculation method for a top cover modal and a pitch diameter of a water turbine, and belongs to the technical field of hydraulic mechanical structure dynamics and simulation automation. The method comprises the steps of parameter input, parametric modeling, automatic simulation control, pitch diameter identification and analysis and result output: after a parameter set is input, constructing a parametric modeling module based on Python and ANSYS Spaceclaim, and automatically generating a three-dimensional geometric model containing a basic panel, a rib plate and a connecting hole; an automatic simulation control module is constructed based on Python and ANSYS Workbench, and full-process automatic simulation is completed to obtain a modal analysis result; pitch diameter characteristic parameters are automatically recognized through a least square discrete Fourier transform algorithm of non-equiangular sampling and confidence degree judgment; and outputting results to form a standardized database. According to the method, automation and quantification of the whole process are achieved, modeling and simulation efficiency is improved, manual misjudgment is avoided, the method is suitable for different types of water turbine top covers, and reliable data support is provided for structural optimization and resonance risk assessment.

The utility model discloses a multi-channel filtering device for a needle type filter, which is characterized by comprising a porous upper supporting plate and a porous lower supporting plate positioned below the porous upper supporting plate, and a plurality of uniformly distributed stepped through holes for placing the filter are formed in the porous upper supporting plate; a plurality of sample introduction bottle positioning grooves corresponding to the stepped through holes are formed in the porous lower supporting plate; the two sides of the bottom of the porous upper supporting plate are symmetrically provided with at least one supporting column, and the two side edges of the porous lower supporting plate are each provided with a supporting column positioning groove which is used for being matched with the supporting column in an inserted mode and provided with a semi-opening. Guide sliding grooves allowing the porous upper supporting plate to horizontally move out of the porous lower supporting plate from the supporting column positioning grooves are formed in the porous lower supporting plate and located between the adjacent stepped through holes, one side of each guide sliding groove is communicated with the corresponding supporting column positioning groove with an inward opening, and the filter has the advantages that the number of filtered samples at a time is large, the filtering efficiency is high, and cross contamination of the filtered samples is avoided.

The invention provides a method and system for testing the electrostatic force of an electrostatic chuck, and the method is applied to a data processing module in the system for testing the electrostatic force of the electrostatic chuck. The system also comprises a to-be-tested electrostatic chuck, a wafer, a pressure sensor, a laser transmitting module and a laser receiving module. The method comprises the following steps: controlling the gas pressure in a gas pipeline to be gradually increased so as to gradually increase the jacking force applied to an adsorbed wafer; the laser reflection condition is monitored through a laser emission module, and when the laser emission module monitors that a laser reflection path deviates, the pressure value of the gas pressure in the gas pipeline detected by a pressure sensor at the moment when the laser reflection path deviates is determined; calculating effective electrostatic force of the electrostatic chuck according to the pressure value; the effective electrostatic force is used for representing the maximum adsorption force of the electrostatic chuck to the wafer. By means of the method, high-precision, high-sensitivity, stable and reliable testing of the electrostatic force of the electrostatic chuck is achieved.

The invention belongs to the technical field of metal explosive cladding manufacturing, and discloses an explosive welding initiating explosive forming mold, an initiating explosive column and an explosive welding method. The mold comprises a pipe body and a cone assembled in the pipe body. The top end of the cone is upward, and a gap is reserved between the bottom face of the cone and the lower end face of the tube body, so that an upper columnar charging cavity and a lower conical energy gathering cavity are formed in the tube body. By means of the simple split design, traditional manual explosive pit digging operation is converted into a standardized process, the energy-gathered initiating explosive column with the highly-consistent shape, size and explosive loading amount is prepared, personal errors are thoroughly eliminated, and repeatability of initiation energy is guaranteed. And meanwhile, the bottom energy gathering cavity structure is beneficial to improving the energy utilization rate, and is particularly suitable for explosive welding of the multi-layer metal plate with the medium thickness. The device has the remarkable technical advantages of simple structure, low cost, time-saving operation, safety, high efficiency, batch production and the like.

The invention relates to the technical field of biological detection, in particular to a kit and method for detecting NfL, the kit comprises a magnetic bead coated with a capture antibody, a non-functional auxiliary magnetic bead, an NfL detection antibody coupled with a first marker, beta-galactosidase coupled with a second marker, an enzyme reaction luminescent substrate and an NfL standard substance; the capture antibody in the magnetic bead coated with the capture antibody is used for being specifically combined with an NfL antigen; the first marker in the NfL detection antibody coupled with the first marker is connected with the second marker in the beta-galactosidase coupled with the second marker, and the NfL detection antibody is suitable for being specifically combined with an NfL antigen. The kit provided by the invention has the advantages of higher sensitivity, less background signal interference, wider linear range, convenience for full-automatic detection instruments and the like.