342results about How to "Improve enrichment capacity" patented technology

The invention provides an enrichment and analysis method for circulating tumor cells. The method mainly comprises the following steps: subjecting a biological fluid sample to red blood cell lysis treatment; adding immune beads specifically bonding with white cells, red cells and/or platelets for incubation; and filtering cell suspension by using a filter membrane and enriching circulating tumor cells. According to the invention, through integration and optimization of CTCs enrichment methods such as red blood cell lysis treatment, an immune bead method and membrane filtration, the enrichment effect of the circulating tumor cells is greatly improved; with the method, the removal rate of leukocytes is greater than 99.9%, and the enrichment rate of tumor cells is greater than 80%. Meanwhile, the invention also provides a kit using the enrichment method for the circulating tumor cells and discloses a device for enrichment of the circulating tumor cells. The invention also provides an analysis method for the enriched circulating tumor cells. With the analysis method, analysis of dynamic changes like the amount of tumor cells, gene mutation and gene expression profiles can be realized.

The invention discloses an analytical detection method of a water bactericide based on a MOFs/TiO2 magnetic composite material. the method comprises the following steps: dispersing magnetic Fe3O4 (at) SiO2 nanospheres into an N,N-dimethylformamide solution of 3-aminopropyltriethoxysilane and succinic anhydride so as to make the surface to carry carboxyl; dispersing the carboxyl-modified Fe3O4 (at) SiO2 nanospheres into a methanol solution of zinc nitrate and 2-methylimidazole to coat the surface with a metal-organic framework material ZIP-8 so as to obtain Fe3O4 (at) SiO2(at) ZIF-8 nanoparticles; and dispersing the Fe3O4 (at) SiO2(at) ZIF-8 nanoparticles into an ethanol solution of tetrabutyl titanate so as to prepare a magnetic nanocomposite material. By the use of the material, the enrichment and separation process becomes simple. By combining advantages of MOFs and TiO2, enrichment efficiency of a bactericide can be raised. The material of the invention can be used in enrichment and separation of a bactericide and liquid chromatography tandem mass spectrometry detection.

The invention belongs to the technical field of inorganic material and analysis, and specifically relates to sea-urchin-shaped core-shell type Fe3O4@TiO2 magnetic microspheres, preparation method and application thereof in the respect of phosphorylated polypeptide selective enrichment. According to the magnetic microspheres, a layer of a SiO2 material is coated on the surface of Fe3O4 magnetic microspheres with a sol-gel method; a layer of anatase nuclei is deposited; and through hydrothermal growth, rutile nano-rods are grown on the surface of the nano-grade nuclei, such that the magnetic microsphere particles with the sea urchin shapes are formed. The magnetic material has large specific surface area, and rapid external magnetic field response. During selective enrichment of trace amount of phosphorylated polypeptide in a biological sample, the treatment is simple, and specificity is high. The material has good practical value and application prospect in the fields such as proteomics.

The invention discloses a tumor cell membrane coated nanometer material, a method for preparing the same and application of the tumor cell membrane coated nanometer material, and belongs to the fieldof nanometer materials. The tumor cell membrane coated nanometer material is particularly a tumor site targeting bionic nanometer material. Cancer cell membranes are coated on the surfaces of mesoporous silicon dioxide nanometer particles (MSN), and glucose oxidase (GOx) is loaded on the MSN, so that hunger treatment can be carried out. The tumor cell membrane coated nanometer material, the methodand the application have the advantages that the surfaces of bionic materials are functionalized, CMSN-GOx has immune escape and homologous targeting capability, and accordingly enrichment of nanometer particles on tumor sites can be obviously improved; tumor can be partially ablated by the prepared CMSN-GOx nanometer particles, and certain antitumor immune response can be generated by means of tumor membrane induction; the tumor can be effectively ablated by the aid of hunger therapy by the CMSN-GOx combined with PD-1 immunotherapy as compared with injection of only PD-1 immunosupressant orthe CMSN-GOx, and adaptive immune response can be induced; the tumor cell membrane coated nanometer material is excellent in biocompatibility and has great clinical application potential.

The present invention discloses a method for repairing soil contaminated by heavy metal by utilizing plant. Said method is characterized by that in the soil contaminated by heavy metal the maize can be planted, before harvest at least two additives selected from editic acid or its sodium salt or its potassium salt (EDTA), nitrilotriacetic acid or its sodium salt or its potassium salt (NTA) and citric aid or its sodium salt or its potassium salt (CA) can be applied, and then the aerial part of maize can be harvested so as to attain the goal of repairing soil contaminated by heavy metal.

The invention discloses a soil heavy metal restoration agent and a preparation method thereof. Modified biomass charcoal impregnated by calcium-rich element and iron and manganese oxides is adopted as the carrier, and a nanoscale magnetic material is employed to coat the carrier. The product has a particle size of 0.25-5mm, and has a Fe content of 2-5%, a Mn content of 5-10%, and a Ca content of 10-15%. The restoration agent has certain restoration effect on single and compound pollution of cadmium, chromium, arsenic, lead and mercury under different pH backgrounds, thus having certain contribution effect on contaminated soil restoration and food safety. The restoration agent prepared by the method provided by the invention can fully and effectively utilize restoration materials with special passivation effect on heavy metals, and achieves the purpose of spectral restoration of heavy metal contaminated soil.

The invention discloses a covalent organic framework material, a solid-phase microextraction probe and a preparation method and application thereof. The covalent organic framework material comprises 2, 3, 5, 6-tetrafluoro-p-dibenzaldehyde and a 2, 4, 6-tri(4-aminophenyl)1, 3, 5-triazine repetitive structure unit which take an imine bond as a connection mode. The solid-phase micro-extraction probeprepared from the material has high selectivity and high enrichment capacity on perfluoro and polyfluoroalkyl compounds, can be directly combined with a nanoliter electrospray ion source for mass spectrometry after sampling extraction, and has extremely high sensitivity, the method can be used for rapid, accurate and sensitive analysis of trace perfluoroalkyl and polyfluoroalkyl compounds in complex environments and biological samples.

The invention relates to a method for rehabilitating uranium contaminated soil by use of a functional plant community. According to the method, broussonetia papyrifera and Ilex cornuta, and reed, Pteris multifida Poir and difformed galingale herb (herbaceous plants), which can enrich uranium in the soil are selected to construct a stable plant community with a function of in-situ repairing for uranium contaminated soil, wherein all plants in the plant community can enrich uranium in the uranium contaminated soil and can transport the uranium into an overground part of the plants, thereby achieving the purpose of rehabilitating the uranium contaminated soil. The method comprises the following steps of firstly, performing seed treatment and sowing; secondly, trimming and fertilizing; thirdly, harvesting; and fourthly, treating in a concentrating manner. The method does not need other auxiliary means and is capable of overcoming the disadvantages that the plant species structure is simple, the plant community is instable and easy to degrade, the maintenance cost is high, the plant diseases and insect pests are more and the ecological functions are at a low level when the single plant species is used for repairing the uranium contaminated soil in an existing method, the five plants have synergistic effect, and the repairing efficiency is improved remarkably compared with that of the method adopting the single plant species for rehabilitating the uranium contaminated soil.

The invention relates to a hypoxia response polymer nanoparticle and application thereof. The method comprises the following steps: firstly crosslinking 4,4-diaminoazobenzene and terephthalaldehyde taken as monomers, so as to form a conjugated polymer link, loading a photosensitizer and chemotherapy drugs through noncovalent interaction, and then forming the hypoxia response polymer nanoparticle through a nanoprecipitation method. The hypoxia response polymer nanoparticle can successfully carry the photosensitizer and the chemotherapy drugs to tumor cells, and produce active oxygen during illumination, so as to realize tumor killing. Azo bonds contained in the nanoparticle can be degraded under the action of reductase in cells, and chemotherapy drug release is realized. In addition, cell hypoxia caused during a photodynamic therapy process can further improve drug release, and the hypoxia response polymer nanoparticle can properly realize excellent tumor therapy effect through combination therapy effect of photodynamic therapy and chemical therapy.

The invention relates to an ore separation process for treating fine-grained slime-containing niobium ore. The process comprises the steps of removing fine slime of the -0.01 mm grain grade under the compound force field effect of a superfine purification grader, performing the combined procedure treatment of strong magnetic rough separation, floating separation and strong magnetic fine separation on settled sand to obtain niobium ore concentrate, and performing regrinding and superfine purification grading on tailings obtained after strong magnetic rough separation and floating separation. By means of the process, the fine slime of the -0.01 mm grain grade can be remarkably removed, the ore pulp environment of magnetic separation and floating separation can be greatly improved, the enrichment effect can be improved, the recovery rate of niobium can be substantially increased, the slime removal process can be simplified, the slime removal process can be improved, and good economic benefits can be brought to enterprises.

The invention discloses red mud-assisted soil remediation fertilizer. The red mud-assisted soil remediation fertilizer is prepared from, by weight, 24-26 parts of wheat gluten flour, 8-10 parts of humus soil, 28-32 parts of cottonseed cakes, 68-74 parts of red mud, 2-3 parts of potassium selenite, 8-10 parts of humic acidic ammonium, 40-45 parts of kapok hulls, 13-15 parts of siderite slag, 13-15 parts of limonite slag, 40-45 parts of orange peel, 4-5 parts of an EM compound microorganism bacterium agent and an appropriate amount of water. According to the soil remediation fertilizer, the red mud materials are utilized to be combined with the humic raw materials, the content of soil nutrients, especially silicon, can be increased, the content of biological effective-state heavy metal is simultaneously decreased, the stabilization effect is improved, accumulation of the heavy metal in organisms is reduced, and the purpose of soil improvement is achieved to enable the soil to be beneficial for crop growth.

The invention discloses a large area MEMS membrane type gas enricher, comprising a silicon substrate, a top cap and an enriching region arranged between the silicon substrate and the top cap; wherein the top cap is provided with an air inlet and an air outlet, the enriching region is composed of a plurality of enriching units, each enriching unit is provided with a hanging membrane which are provided with a film heater and an adsorption film, and a silicon frame which are arranged all around the hanging membrane. The invention not only has the advantages of small heat capacity and fast heating rate but also effectively increases absorption area and absorption gross and improves enriching rate by combining a plurality of enriching units.

The invention relates to an environmental analysis detection method, and specifically, relates to a high performance liquid chromatography determination method of tetracycline antibiotic in soil. The high performance liquid chromatography determination method of tetracycline antibiotic in soil comprises the following steps of 1, a sample collecting and preparation process comprising collecting soil in a plough layer having thickness of 0 to 20 centimeters, wherein soil in a composting place and field edges is not adopted, uniformly mixing multiple single-point samples, carrying out quarteringreduction to obtain a mixed soil sample having weight of about 1kg, putting the mixed soil sample into a sealing bag, bringing the sealing bag with the mixed soil sample back with low-temperature preservation in the dark, putting the mixed soil sample into a room, drying in air, crushing, screening by a nylon sieve of 60 meshes, and weighing 2g of the mixed soil sample for next use, 2, an extractpreparation process, 3, an extraction, purification and enrichment process, and 4, an elution, volume metering and detection process. The high performance liquid chromatography determination method of tetracycline antibiotic in soil has normalized processes, reduces a vacuum drying process, produces low jamming, shortens operation time and improves operation accuracy. Through connection between astrong anion solid phase extraction column and a C18 inverted solid phase extraction column in series, purification and enrichment effects are greatly improved.

The invention discloses a rolling circle amplification DNA enzyme and covalent organic framework-based electrochemical-ELISA immunosensor, and belongs to the technical field of electrochemical detection. An electrochemical method and enzyme-linked immunosorbent assay are combined, a nucleic acid signal is amplified on an ELISA plate, an electrode is modified with a functional nanometer material, linear dependence between an electrical signal of a reduction peak and aflatoxin M1 within a range being 0.5-80ng/mL is generated, an association coefficient is 0.993, and the detection limit is 0.15ng/mL (S/N=3). By the rolling circle amplification DNA enzyme and covalent organic framework-based electrochemical-ELISA immunosensor, the electrode passivation in traditional electrochemical analysis can be prevented, an ELISA signal also can be effectively amplified, amplification of a cascading signal is achieved, the detection limit is reduced, trace detection is achieved, and a high-sensitivity, high-selectivity and high-throughput electrochemical ELISA method is built.

The invention discloses organic polluted soil remediation fertilizer which is prepared from, by weight, 10-12 parts of shell powder, 23-25 parts of bone meal, 5-6 parts of polyaspartic acid, 14-16 parts of powdered straw sugar, 1-3 parts of potassium chlorate, 2-3 parts of compound sodium nitrophenolate, 34-36 parts of eucalyptus saw powder, 13-14 parts of siderite slag, 13-14 parts of limonite slag, 55-60 parts of orange peel, 5-6 parts of EM compound microorganism bacterium agents, and an appropriate amount of water. The soil remediation fertilizer is applicable to deodorization and soil regeneration for soil polluted by waste in a brewery, a slaughter factory, a livestock farm and the like, water and oil in the pollutant are adsorbed and gathered through organic strong adsorption materials such as the shell powder and the bone meal, and microbial decomposition is carried out in an auxiliary mode, so that the ecological environment of the polluted soil is greatly improved, pathogenic bacteria breeding is prevented, the improved soil can be used as a seedling culture substrate, and the utilization value of the improved soil is improved.

The invention relates to a headspace gas chromatography detection technology, particularly discloses a vapor headspace enrichment detection method for a trace amount of volatile organic pollutants in seawater, and belongs to the technical field of water environment detection. The method sequentially comprises the four steps of water sample collection and storage, primary enrichment condensation, secondary enrichment condensation and headspace gas chromatography detection. According to the method, water vapor is used as sweeping gas, water is used as an absorbent, a water sample is secondarily distilled and condensed, and the condensed sample is enriched for headspace gas chromatography detection; a vapor headspace enrichment method is adopted, so that the using amount of an organic solvent in a process of enriching the volatile organic pollutants in the seawater is effectively reduced, and the interference of other components to a target object is prevented; an extraction process of the enrichment method is simple and convenient, the introduction of other solvents is avoided, the method is environment-friendly, low in limit of detection and high in recovery rate and reproducibility, and the requirements of detection of a trace amount of volatile organic pollutants in the seawater can be met.

The invention discloses a metallic-organic gel matrix solid-phase microextraction head with multiple levels of hole channels. The solid-phase microextraction head is characterized by being composed of a steel wire and a solid-phase microextraction coating layer wrapped on the surface of the steel wire, wherein the solid-phase microextraction coating layer comprises the metallic-organic gel with the multiple levels of hole channels. The metallic-organic gel with the multiple levels of hole channels is used as the coating material of the solid-phase microextraction head, so that the enriching capability of the solid-phase microextraction head for gathering polar materials is enhanced by means of the multi-hole structure, the adjustable hole diameter and the modifiable hole surfaces of the metallic-organic gel.

The invention discloses a daily feed for laying hens producing eggs enriched with omega-3 polyunsaturated fatty acid. The daily feed for the laying hens producing eggs enriched with omega-3 polyunsaturated fatty acid is mainly prepared from the following raw materials in parts by weight: 10-20 parts of barley, 5-10 parts of fermented soybean meal, 2-4 parts of beer yeast, 5-10 parts of puffed soybeans, 2-5 parts of puffed linseeds, 3-6 parts of oat bran, 3-6 parts of alfalfa, 2-5 parts of ultrafine wall-broken grape seeds, 2-8 parts of seaweed powder, 0.5 part of a compound premix, 0.003-0.005 part of selenium yeast, 0.05-0.1 part of a compound enzyme preparation, 0.03-0.08 part of betaine, 0.05-0.1 part of probiotics, 0.3 part of Boshan X-1, 0.01-0.03 part of phytase, 0.03-0.1 part of lipase, vitamins, amino acids, and so on. Being used for breeding laying hens, the daily feed is capable of improving immunity and disease resistance of the laying hens, as well as increasing content of omega-3 polyunsaturated fatty acid, especially content of DHA, in the eggs.

The invention discloses a modified biochar based composite Fenton catalyst and a pollution treatment method. The composite Fenton catalyst is characterized in the following steps: placed the biochar in a copper sulfate solution having a concentration of 10-50 mmol/L, stirring or oscillating the biochar and the copper sulfate solution for absorption for 180 min, carrying out centrifugation, and placing the mixture into a tubular furnace or muffle furnace, and carrying out heat treatment at 550-850 DEG C under nitrogen protection for 0.5-2 h to obtain the modified biochar; mixing the modified biochar and pyrite at a mass ratio of 1-5:1, and grinding the modified biochar and pyrite until the particle size is less than 0.15 mm to obtain the composite Fenton catalyst; and adding the composite Fenton catalyst of 10-60 g/L into wastewater containing organic pollutants in order, evenly stirring and mixing, adding persulfate of 3-15 mmol/L, and performing a Fenton oxidation reaction for 20-120min. The modified biochar based composite Fenton catalyst and the pollution treatment method can effectively improve the degradation efficiency of the organic pollutants.

The invention relates to a macromolecular biochar sphere-immobilized microalgal composite adsorbent and preparation and application thereof; the macromolecular biochar spheres are a spherical support formed under specific conditions by adding a macromolecular polymer to biochar that serves as a main material; microalgae and biochar spheres are mixed in a certain ratio, microalga-secreted extracellular polymer is used as a binder under the cooperation of lighting, nutrition and the other conditions, the binder reacts with developed pores in the surfaces of the biochar spheres to adsorb micrometer-level microalgal cells, and accordingly, the advantage of the biochar spheres to quickly adsorb heavy metals in wastewater is made full use, it is possible to further enrich the heavy metals in mixed wastewater via growth and metabolism of algal cells so as to consume nitrogen, phosphorus and other eutrophic nutrients in the wastewater. The method allows the nitrogen and phosphorus in wastewater to be effectively removed and heavy metals to be also removed, and has the advantages of zero secondary pollution, good production process simplicity, good availability and the like.

The invention discloses a soil restoration fertilizer with the passivated heavy metal mobility. The soil restoration fertilizer with the passivated heavy metal mobility is prepared from, by weight, 20-24 parts of paulownia sawdust, 34-36 parts of Chinese medicine residues, 4-5 parts of gelatin, 16-18 parts of kaolin, 1-2 parts of sodium polyphosphate, 1-2 parts of polymeric aluminum chloride, 1-3 parts of zinc chloride, 48-50 parts of eucalyptus sawdust, 12-14 parts of siderite slag, 12-14 parts of limonite slag, 45-55 parts of orange peel, 3-4 parts of an EM composite microbial agent and a proper amount of water. The soil restoration fertilizer is modified through mixed calcination of kaolin and sodium polyphosphate, the buffering capacity of soil in use is improved, the adsorption capacity of soil for heavy metal is enhanced, migration of heavy metal in soil to plants is inhibited, eating safety of crop products is guaranteed, meanwhile, the conditions of leaching, infiltration and the like of heavy metal can be reduced, underground water resources are prevented from being polluted, and health of a soil ecological environment is guaranteed.