Microorganism passivator for repairing heavy metal pollution of soil and preparation and application thereof

A technology for remediating soil and microorganisms is applied in the field of microbial passivators for remediating heavy metal pollution in soil, which can solve the problems of ecological environment damage and high cost, and achieve the effects of reducing pollution, increasing the number of viable bacteria and shortening production time.

Inactive Publication Date: 2017-06-20
SHANGHAI LVLE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Peat is a short-term non-renewable natural mineral resource, long-term mining will cause great damage to the ecological environment, and the cost is high; diatomaceous earth and vermiculite are natural, inorganic, non-toxic minerals that do not contain Nutrients needed by living bacteria

Method used

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  • Microorganism passivator for repairing heavy metal pollution of soil and preparation and application thereof
  • Microorganism passivator for repairing heavy metal pollution of soil and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The present embodiment relates to the preparation method of the microbial inactivator of repairing soil heavy metal pollution, comprises the steps:

[0052] 1. Strain fermentation

[0053] Bacillus subtilis (Bacillus subtilis) and Bacillus megaterium var. phosphaticum (Bacillus megaterium var. phosphaticum) were respectively subjected to slant culture, shaker expansion culture, seed tank fermentation and fermenter fermentation, and mixed to obtain fermentation liquid, the activity of the fermentation liquid The number of bacteria reached 4.6 billion / ml;

[0054] 2. Carrier processing

[0055] The hydrolyzed slag was obtained from Henan Lianhua Monosodium Glutamate Co., Ltd.

[0056] Crush the large pieces of hydrolyzed slag into powder or small particles, mix with powdered lime according to the ratio of hydrolyzed slag:lime=2:1, crush, pile up the powdered material for 10 hours, and then sieve and bag it to make it The pH reaches 7.0-7.8. ; Mix the pretreated biocha...

Embodiment 2

[0062] The present embodiment relates to the preparation method of the microbial inactivator of repairing soil heavy metal pollution, comprises the steps:

[0063] Bacillus subtilis (Bacillus subtilis) and Bacillus megaterium var. phosphaticum (Bacillus megaterium var. phosphaticum) were respectively subjected to slant culture, shaker expansion culture, seed tank fermentation and fermenter fermentation, and mixed to obtain fermentation liquid, the activity of the fermentation liquid The number of bacteria reached 4.6 billion / ml;

[0064] Crush the large piece of hydrolyzed slag into powder or small particles, mix it with powdered lime according to the ratio of hydrolyzed slag:lime=3:1, and pulverize it. After the powdered material is piled up for 15 hours, carry out the second pulverization, and then pass Sieve bag, its pH reaches 7.0-7.8. Mix the pretreated biochar and dilute nitric acid with a mass fraction of 10% at a ratio of 1 (Kg): 10 (L), stir in a blender for 8 hours,...

Embodiment 3

[0068] The present embodiment relates to the preparation method of the microbial inactivator of repairing soil heavy metal pollution, comprises the steps:

[0069] Bacillus subtilis (Bacillus subtilis) and Bacillus megaterium var.phosphaticum (Bacillusmegateriumvar. 4.6 billion / ml;

[0070]Crush the large pieces of hydrolyzed slag into powder or small particles, mix them with powdered lime according to the ratio of hydrolyzed slag:lime=2:1, and pulverize them. After the fine powder is piled up for 10 hours, carry out the second pulverization, and then pass Sieve bag, its pH reaches 7.0-7.8. Mix the pretreated biochar and dilute nitric acid with a mass fraction of 10% at a ratio of 1 (Kg): 10 (L), stir in a stirrer for 12 hours, filter and wash the filter residue repeatedly with distilled water until the pH is neutral. The treated hydrolyzed slag and biochar are mixed and pulverized at a mass ratio of 3:7 to prepare the carrier.

[0071] The mixed Bacillus subtilis (Bacillus...

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Abstract

The invention discloses a microorganism passivator for repairing heavy metal pollution of soil and preparation and application thereof. A preparing method comprises the steps of fermentation and adsorption of strains. Specifically, bacteria solutions obtained after fermentation of bacillus megateriumvar.phosphaticum and bacillus subtilis LVLE-14 are mixed in proportion, then adsorption is conducted by means of a special adsorption carrier, and then mixing and smashing are conducted to obtain the microorganism passivator. The adsorption carrier comprises hydrolysis residues and biochar which are mixed and smashed according to a certain mass ratio. By the adoption of the microorganism passivator for repairing heavy metal pollution of soil, the content of available heavy metals in soil can be effectively reduced, and the number of microbial populations and plant yield are increased greatly. The method for repairing heavy metal pollution of soil by means of the microorganism passivator is in-situ repair, operation is easy, the cost is low, and industrialized implementation can be easily achieved; furthermore, the repairing effect is good, soil fertility can not be damaged, and the utilization rate of resources can be increased.

Description

technical field [0001] The invention relates to the technical restoration field of soil microorganisms, in particular to a microbial passivator for repairing heavy metal pollution in soil and its preparation and application. Background technique [0002] Due to the extensive use of industrial "three wastes", sewage irrigation, agricultural sludge and agricultural inputs containing heavy metals, soil heavy metal pollution has become increasingly serious and has become one of the major environmental problems in my country. Among the eight heavy metal elements such as cadmium (Cd), arsenic (As), lead (Pb), mercury (Hg), chromium (Cr), copper (Cu), zinc (Zn), nickel (Ni)), Cd, As It is highly toxic and has been classified as a Class I carcinogen by the International Agency for Research on Cancer (IARC). According to the "National Soil Pollution Survey Bulletin" jointly issued by the Ministry of Environmental Protection and the Ministry of Land and Resources in 2014, my country'...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/11C12R1/125
CPCC12N1/20B09C1/10B09C2101/00
Inventor 王小蒙陈露闫龙翔
Owner SHANGHAI LVLE BIO TECH
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