1189 results about "Bacillus subtilis" patented technology

The invention discloses a multifunctional low-temperature corn straw degradation complex microbial inoculant, a preparation method and application, the complex microbial inoculant is compounded by a plurality of microorganisms with straw degradation, potassium dissolving and growth promoting functions, and the complex microbial inoculant comprises the following strains: bacillus subtilis K15, curveolate Prickia L11 and bacillus mycoides L31. The complex microbial inoculant disclosed by the invention realizes synergistic interaction and functional complementation, and through scientific compounding of strains, the straw decomposition rate is remarkably increased, and the decomposition period is obviously shortened; the adaptive capacity and colonization competitiveness of microorganisms in a low-temperature and complex soil environment in winter are enhanced. The complex microbial inoculant disclosed by the invention also has multiple functions of degrading straws, dissolving potassium, producing IAA (Indoleacetic Acid) and the like. The soil potassium which is difficult to utilize can be converted into quick-acting potassium while the straws are quickly decomposed to release nutrients.

The invention belongs to the technical field of agriculture, and particularly provides a compound microbial agent and application thereof. The compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02. The compound microbial agent can effectively improve the pH and EC of saline alkali soil, increase the content of alkali-hydrolyzable nitrogen, available phosphorus and quick-acting potassium in the soil, and play a significant role in increasing the plant height, stem diameter, chlorophyll content and aboveground biomass of corn plants in coastal saline-alkali soil.

The invention discloses a fermentation method of recombinant bacillus subtilis for expressing vibrio parahaemolyticus outer membrane protein, which comprises the following steps: inoculating a recombinant bacillus subtilis strain into a liquid culture medium for activation and amplification to obtain a seed solution; transferring the seed solution into a fermentation tank according to the inoculum size of 5%-10%, controlling the temperature to be 35-37 DEG C and the pH to be 6.8-7.2, introducing sterile air and stirring, and monitoring the thallus concentration OD600 value in real time; when the OD600 value reaches 35-38, 10% of an inducer is added for pre-induction; when the OD600 value reaches 40-45, the remaining 90% of the inducer is added for main induction, and the real-time dissolved oxygen value is collected; when the real-time dissolved oxygen value is continuously higher than a first preset threshold value, the flow acceleration rate of the inducer is increased, the fermentation temperature is increased at the same time or step by step, and the increase amplitude of the dissolved oxygen value is in positive correlation with the flow acceleration rate and the temperature increase amplitude; when the OD600 value is stabilized in a preset interval, it is judged that fermentation is completed, and the recombinant bacillus subtilis thalli are obtained. The yield and quality of the vibrio parahaemolyticus outer membrane protein can be effectively improved.

The invention belongs to the technical field of hair care products, and particularly relates to composite acid gel for scalp care and a preparation method thereof. Salicylic acid, glycolic acid, piroctone azelate olamine salt, echinacea purpurea-lactic acid bacteria yeast, an emulsifier and water are combined, wherein the echinacea purpurea-lactic acid bacteria yeast is prepared by performing enzymolysis on echinacea purpurea through bacillus subtilis protease and then performing aerobic fermentation with lactic acid bacteria. Salicylic acid, glycolic acid and azelaic acid in the composite acid gel are compounded, so that stimulation of salicylic acid is weakened, and the effect of dissolving hair follicle grease is reserved; glycolic acid and azelaic acid are added to accelerate keratin metabolism, regulate sebum secretion and create safe and healthy shielding for the scalp environment, and polysaccharide and flavonoid compounds in the echinacea purpurea-lactic acid bacteria leavening are beneficial to exerting the anti-oxidation and immune regulation effects of the echinacea purpurea-lactic acid bacteria leavening. Therefore, the composite acid gel provided by the invention is green, safe and non-irritant, the preparation method is simple and convenient, and the composite acid gel can be used for inhibiting bacteria and reducing scalp inflammation.

The invention discloses an endoglucanase truncated mutant and an application of the endoglucanase truncated mutant. According to the invention, based on endoglucanase BsEGL from bacillus subtilis DLG, a truncated mutant is constructed by deleting a carbohydrate binding domain (CBM) of the endoglucanase BsEGL, and the truncated mutant is named as BsEGL [delta] CBM. Compared with BsEGL, the truncated mutant BsEGL delta CBM has the advantages that the thermal stability, the pH stability, the organic solvent tolerance and the like are remarkably improved, and the activity of degrading phosphoric acid expanded cellulose and corncob xylan is obtained. In addition, the enzymatic activity of the truncated mutant BsEGL delta CBM is remarkably improved by 6.41%, and the catalytic efficiency is remarkably improved. Due to the characteristics, the mutant can be suitable for industrial processes such as feed processing, papermaking, biological energy sources, spinning and food brewing, and is particularly suitable for enzyme catalytic reaction under extreme environments such as high temperature and low pH. The invention provides an enzyme preparation tool with excellent performance for efficient degradation of cellulose biomass, and has important application value.

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The invention discloses keratinase Phyto-Keratinase and application of the keratinase Phyto-Keratinase. The keratinase Phyto-Keratinase comprises the following components: (1) protease which is coded by a nucleotide sequence as shown in SEQ ID NO.1; and / or (2) protease which is derived from bacillus subtilis, is coded by a nucleic acid sequence with more than 98% of identity with the SEQ ID NO.1 nucleotide sequence and has the characteristic of hydrolyzing keratin. According to the plant keratinase Phyto-Keratinase disclosed by the invention, the Phyto-Keratinase (plant keratinase) produced by utilizing microbial fermentation is a serine proteolytic enzyme, is also a biocompatible cutinase, and is obtained by fermenting saccharomyces cerevisiae. Phyto-Keratinase provides a nitrogen source for the growth of plants (beans), and contains various amino acids (functional peptides) and special proteolytic enzymes derived from the plants at the same time. The Phyto-keratinase can provide the effects of resisting inflammation, removing cutin and providing nutrition for skin regeneration at the same time, can relieve various inflammations by inhibiting PAR-2 receptors, and has potential value in the fields of cosmetics, beauty medicine and environment.

The invention discloses a bacillus subtilis strain and application thereof in prevention and treatment of tomato brown wrinkled fruit virus diseases. According to the bacillus subtilis strain, the bacillus subtilis strain is NA25, the preservation name is bacillus subtilis, the bacillus subtilis strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 27, 2025; the preservation number of the strain is CGMCC No. 34380. The bacillus subtilis provided by the invention is separated from healthy tomato endophyte, and the strain fermentation liquor and cell suspension both have a good inhibition effect on the brown wrinkled fruit virus, and can be used for preventing and treating the tomato brown wrinkled fruit virus.

The invention belongs to the technical field of synthetic biology, and particularly relates to recombinant escherichia coli for efficiently producing O-acetyl-L-homoserine as well as a construction method and application of the recombinant escherichia coli. According to the invention, CRISPR-Cas9 gene editing is combined with metabolic modification, so as to precisely integrate OAH biosynthesis key nodes: introducing and overexpressing a mutant metX gene at a genome pseudogene site to enhance the expression of homoserine acetyltransferase, knocking out byproducts such as poxB and mgsA to synthesize genes, and carrying out high-yield synthesis of OAH. Corynebacterium glutamicum thrA (anti-feedback inhibition), aspB and bacillus subtilis acsA-acuA genes (construction of an acetic acid switch) are introduced, gene expression of gltA, tpiA and the like is optimized to increase supply of precursors such as acetyl CoA and the like, and finally a stable strain without plasmids, antibiotics and inducers is obtained. The production cost is effectively reduced, and the method has the advantage of high genetic stability and shows a good application prospect in OAH industrial production.

The invention discloses bacillus orientalis ZL3 for regulating rhizosphere soil microecosystems of haloxylon ammodendron soil in deserts and promoting the growth of the haloxylon ammodendron soil, particularly relates to a fungicide ZL3 for promoting the growth of haloxylon ammodendron and regulating rhizosphere microbial communities and application of the fungicide ZL3, and belongs to the technical field of microorganisms. According to the bacillus toonensis ZL3 provided by the invention, the conversion of soil nutrients to available nutrients is improved, and the rhizosphere microbial community structure is improved, so that the effect of promoting the growth of haloxylon ammodendron is achieved. When the ZL3 is applied to a haloxylon ammodendron field, the content of soil organic matters, the content of soil quick-acting potassium, the content of available phosphorus and the activity of sucrase are remarkably improved by 37.78%, 11.01%, 49.66% and 67.71% respectively. The plant height is increased by 17.59%, and the root length is increased by 109.45%. Meanwhile, the positive change of microbial communities is regulated and controlled, especially the enrichment of beneficial bacteria, the relative abundance of the prevotella and the vibrio is remarkably increased, and the relative abundance of the burkholderia is remarkably reduced. In general, inoculation of the ZL3 fungicide can promote the growth and development of haloxylon ammodendron by improving the soil fertility and improving the rhizosphere microflora structure.

A method for improving degradation efficiency of corn straw at low temperature comprises the steps that cell-free culture fluid PfCFs prepared from Pseudomonas fragi D12 participates in fermentation and degradation of the corn straw, the classification name of the strain D12 is Pseudomonas fragi D12, the strain D12 is preserved in China Center for Type Culture Collection, the preservation address is No. 299, 8th road, Wuchang District, Wuhan City, Hubei Province, China, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) NO: M2025126, and the preservation date is January 14, 2025. The acellular culture solution of the pseudomonas rubrassicae D12 can promote the growth of various normal-temperature bacteria and fungi functional bacteria at low temperature and improve the cold resistance of the functional bacteria, and CFS of the pseudomonas rubrassicae D12 also contains related enzymes capable of degrading lignin; at a low temperature, the PfCFs synchronously promotes the co-growth of bacteria bacillus subtilis and fungi Irpex lacteus, so that the degradation efficiency of the functional bacteria on lignocellulose in the corn straws is improved.

The invention belongs to the technical field of biological fermentation, and particularly relates to ganoderma lucidum polypeptide and a preparation method and application thereof. The preparation method comprises the following steps: (1) taking ganoderma lucidum powder, adding water, and cooking to obtain a mixture 1; (2) adding bacillus subtilis liquid into the mixture 1, and fermenting to obtain ganoderma lucidum polypeptide fermentation liquid bacteria liquid; (3) adding neutral protease into the ganoderma lucidum polypeptide fermentation liquid for primary enzymolysis, and then adding aminopeptidase for secondary enzymolysis to obtain ganoderma lucidum polypeptide enzymatic hydrolysate; and (4) filtering, concentrating and spray-drying the lucid ganoderma polypeptide enzymatic hydrolysate to obtain the lucid ganoderma polypeptide. The molecular weight of the ganoderma lucidum polypeptide ranges from 200 Da to 5000 Da, molecular weight distribution is uniform, the polypeptide content can reach 31.11% at most, the ganoderma lucidum polypeptide can be applied to functional cosmetics, and the application range of the ganoderma lucidum polypeptide is widened.

The invention relates to a sodium hyaluronate fermentation filtrate as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. Through a two-stage fermentation mode, the fermentation filtrate containing sodium hyaluronate is prepared by recombining bacillus subtilis, and then residual nutrient substances in a culture medium are consumed by inoculating bacillus velezensis, so that the nutrient substances are metabolized into active substances with repairing and strengthening effects on hair; the prepared sodium hyaluronate fermentation filtrate has the effects of repairing scalp and strengthening hair. The sodium hyaluronate filtrate disclosed by the invention can be used for well reducing the content of NO in macrophages (the NO content of cells is increased due to malassezia lysate), can be used for removing active oxygen ROS of HaCaT cells and increasing the total tensile strength of hair, and has the potential effects of relieving scalp, resisting oxidative stress and enhancing the toughness of the hair.

The invention belongs to the technical field of food processing, and relates to a method for preparing low-salt shrimp juice through rapid microbial fermentation. The method comprises the following steps: removing heads of shrimps, preparing meat paste, drying and crushing shrimp shells, and mixing the shrimp shells with the meat paste; carrying out enzymolysis treatment on the meat paste by using alkaline protease, and then carrying out high-temperature enzyme deactivation; mixing the shrimp meat and shrimp shell enzymatic hydrolysate mixture with soybean meal and bran, and sterilizing at high temperature; inoculating a complex microbial inoculant containing lactobacillus plantarum, saccharomyces rouxii, aspergillus oryzae, deep-sea bacillus subtilis and bacillus subtilis to prepare a mold starter, fermenting at 28 DEG C for 30 days, and finally filtering and sterilizing. According to the method disclosed by the invention, the availability of the raw materials is effectively improved, the fermentation period is shortened, efficient fermentation under a low-salt condition is realized, various basic indexes of the obtained shrimp juice product meet national standards, and the shrimp juice product is outstanding in flavor quality and has rich sauce flavor and sweet taste. In addition, the shrimp juice shows good antioxidant stability before and after digestion.

The invention provides a bacillus subtilis strain and application thereof, the bacillus subtilis strain is named as bacillus subtilis Ya3.1, is preserved in China General Microbiological Culture Collection Center on July 7, 2025, and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35019; the bacillus subtilis Ya3.1 is separated from intestinal tracts of healthy hybrid sturgeons, and can be used as a probiotic additive for aquaculture, especially for sturgeon culture. The bacillus subtilis Ya3.1 provided by the invention has high safety, strong stress resistance and efficient intestinal colonization ability, and can significantly improve the activity of sturgeon intestinal digestive enzymes, improve the growth performance of sturgeons, enhance the non-specific immunity of sturgeons, improve the intestinal flora structure and significantly improve the ability to resist aeromonas bacterial infection; and a special probiotic resource can be provided for sturgeon culture.

The invention discloses bacillus subtilis, a method for preparing yellow corn silage feed under a strong alkali condition and a product thereof, and belongs to the technical field of microorganisms. The technical problem to be solved is that the survival rate of the existing bacillus subtilis is lower than 30% under the strong alkali condition of pH 9-11, and the existing bacillus subtilis cannot normally grow and propagate. The preparation of the yellow corn silage feed under a strong alkali condition needs additional pH adjustment, the process is complex and the cost is high; the crude protein content of the existing yellow corn silage feed is only 3-5%, and the requirements of livestock cannot be met. According to the technical scheme, the strong-alkali-resistant bacillus subtilis is provided, the preservation number is CGMCC No.36677, the survival rate of the strain is larger than or equal to 80% under the condition that the pH is 9-11, the straw degradation rate is larger than or equal to 25%, no waste water is generated when the strain is used for preparing the yellow silage feed, the cost is reduced compared with an existing technology, the protein content is increased by 50%-80%, palatability is good, and the method is suitable for large-scale straw resource utilization and feed protein production.

The invention provides a preparation method of aquatic organism bottom modification particles, which comprises the following steps: adding bacillus subtilis liquid, an oxygen producer and an adhesive into a base material, stirring and granulating to obtain oxygen release core particles; stirring the oxygen release core particles in a roller coating machine, and intermittently spraying a chelating agent in the stirring process to obtain transition particles; and spraying the heavy metal adsorption sol on the transition particles, spraying the bonding solution after cross-linking and curing, then roll-coating the counterweight particles, and drying to obtain the aquatic organism bottom-changing particles. Reducing harmful gas and nitrogen and oxygen indexes are reduced, heavy metals and fine suspended solids are captured synchronously, and the oxidation-reduction buffer capacity of the bottom mud and the stability of a water body are recovered.

The invention relates to the technical field of fruit and vegetable preservation, in particular to a calcium ion reinforced bacillus subtilis fermentation preservative and application thereof in fresh-cut vegetables. The preparation method of the calcium ion reinforced bacillus subtilis fermentation liquor comprises the following steps: collecting the bacillus subtilis fermentation liquor cultured for 120 hours, weighing a certain mass of calcium salt according to the volume of the fermentation liquor, adding the calcium salt into the fermentation liquor, quickly stirring until the calcium salt is dissolved, sealing, reacting at constant temperature for a certain time, centrifuging, collecting supernate, and drying to obtain the calcium ion reinforced bacillus subtilis fermentation liquor. And freezing and drying the supernate to obtain the antibacterial preservative. The calcium ion reinforced bacillus subtilis fermentation liquor prepared by the invention has broad-spectrum and efficient antibacterial performance, can effectively maintain the fresh sensory quality of fresh-cut radishes and fresh-cut cabbages, effectively delays the quality aging and deterioration of the fresh-cut radishes and fresh-cut cabbages, and achieves the purpose of prolonging the shelf life of fresh-cut vegetables. The preservative is suitable for preservation of fruits, vegetables and fresh-cut vegetables, solves the problems of narrow antibacterial spectrum and short action time of fermentation liquor, and has a good application effect.

The invention discloses a preparation method of chitosan oligosaccharide. The method comprises the following steps: adding chitin into concentrated hydrochloric acid, grinding until the chitin is dissolved into paste, washing with distilled water, and collecting to obtain a white colloidal substance, namely colloidal chitin; dissolving colloidal chitin in a phosphoric acid-citric acid buffer solution to obtain a mixed dissolved solution; adding a crude enzyme solution of composite chitinase into the mixed dissolving solution, placing the mixed dissolving solution in a shaking table for reaction, terminating the reaction in a boiling water bath, centrifuging, and carrying out activated carbon separation, low-temperature rotary evaporation concentration and freeze drying on supernate to obtain a chitosan oligosaccharide finished product. According to the method, chitinase crude enzyme liquid prepared from bacillus subtilis SH21 and chitinase crude enzyme liquid prepared from penicillium oxalicum K10 are used as compound enzymes, chitin is subjected to enzymolysis to prepare the chitosan oligosaccharide, the polymerization degree of the prepared chitosan oligosaccharide is 3-10, trisaccharide, tetrasaccharide, pentasaccharide and hexasaccharide are mainly used, and compared with single enzyme use, the compound enzymes are high in product yield and high in chitin degradation rate.

The invention relates to a method for improving fermentation production efficiency of bacillus subtilis lipopeptide sodium by controlling threonine flow acceleration rate based on fermentation process oxygen uptake rate curve feedback. Bacillus subtilis is used as a production strain, and lipopeptide sodium is produced through deep liquid state fermentation. In the fermentation process, the oxygen uptake rate of the process is detected, a threonine and ammonium sulfate continuous feeding control process based on oxygen uptake rate curve parameters is implemented, the oxygen uptake rate in the fermentation process is stably maintained to be 60-65 mmo1 / L / h, the ammonium ion concentration is maintained to be 15-20 mmol / L, and rapid growth of hyphae and rapid increase of the lipopeptide sodium synthesis rate can be achieved. The threonine and ammonium sulfate fed-batch control process based on oxygen uptake rate feedback control is established, the production period is shortened, and the fermentation production level is stably improved.

The invention discloses a mycobacteriophage and application thereof, and relates to the technical field of bacteriophages. The mycobacteriophage is named as Mycobacter phage BZNK001, is preserved in Guangdong Microbial Culture Collection Center, has a preservation number of GDMCC No: 67907-B1, does not contain antibiotic drug-resistant genes and integrase genes, has excellent biological safety and good environmental tolerance, can maintain high biological activity in a temperature range of 37-50 DEG C under an acid-base condition of pH 5-9, and can be used for preparing the antibiotic drug-resistant gene and integrase gene-containing mycobacteriophage for preventing and treating the antibiotic drug-resistant gene and integrase gene-containing mycobacteriophage for preventing and treating the antibiotic drug-resistant gene and integrase gene-containing mycobacteriophage. The bacillus subtilis has broad-spectrum and high-efficiency non-tuberculous mycobacterium cracking activity, and can effectively crack four clinical key mycobacteria such as mycobacterium abscessus, mycobacterium avium, mycobacterium intracellularis and mycobacterium smegmatis. According to the invention, a mycobacteriophage resource library is enriched, a novel and stable biological prevention and control means is provided for efficient prevention and control of mycobacteria, and the mycobacteriophage has good clinical application and industrialization values.

The invention discloses a trans-boundary synthetic flora (SMC) for preventing and treating wheat stem rot as well as preparation and application of the trans-boundary synthetic flora. The synthetic bacterial flora is prepared by compounding Trichoderma harzianum T19 with the preservation number of CCTCC (China Center for Type Culture Collection) No: M 20251896, bacillus subtilis BS-Z15 with the preservation number of CCTCC No: M 20251892, two strains of growth promoting bacillus PM9 and PM16, and two strains of auxiliary bacillus S8 and S32 according to a specific proportion. Compared with an existing single antagonistic bacterial agent, the synthetic flora disclosed by the invention has the advantages that the bacteriostasis rate of the synthetic flora on Fusarium pseudograminearum under a non-sterile soil condition can reach 70% or above, and the disease index is reduced by 70.01%; meanwhile, the plant height of wheat is increased by 28.63%, the root length is increased by 24.53%, the fresh weight is increased by 181.21%, the dry weight is increased by 184.64%, the thousand seed weight is increased by 10.39%, and the rhizosphere microbial community structure and the soil enzyme activity are remarkably improved. The method can be used for green prevention and control and yield increase of basal stem rot of wheat and other gramineous crops.

The invention provides a preservation method of fresh-cut purple sweet potatoes, which comprises the following steps: peeling and slicing the purple sweet potatoes, carrying out heat treatment at 50 DEG C for 5 minutes, soaking in a fermentation extracting solution containing 50% of bacillus subtilis, 50% of garlic extracting solution, 0.4% of calcium chloride, 0.04% of melatonin and 0.3% of citric acid, treating for 15 minutes, airing, carrying out vacuum packaging, and finally refrigerating and storing at 4 DEG C. The method effectively inhibits browning of the fresh-cut purple sweet potatoes, maintains the color, hardness and nutritional ingredients of the fresh-cut purple sweet potatoes, and prolongs the shelf life. The method has the advantages of greenness, harmlessness, simplicity in operation, remarkable preservation effect and the like, and a new technical scheme can be provided for preservation of the fresh-cut purple sweet potatoes.

The invention discloses a compound rumen bypass guanidinoacetic acid additive as well as a preparation method and application thereof, and belongs to the technical field of animal feed additives. The compound rumen bypass guanidinoacetic acid additive provided by the invention is prepared from the following components in parts by mass: 40 to 60 parts of guanidinoacetic acid, 0.1 to 0.5 part of selenium yeast, 5 to 10 parts of nicotinamide, 5 to 12 parts of DL-methionine, 0.01 to 0.05 part of vitamin B12, 1 to 3 parts of compound probiotics, 3 to 5 parts of hydroxypropyl methyl cellulose, 1 to 2 parts of ethyl cellulose, 15 to 25 parts of hydrogenated palm oil and 3 to 5 parts of glyceryl monostearate. The compound probiotics comprise bacillus subtilis and saccharomyces cerevisiae. The compound additive provided by the invention can significantly improve the daily gain of ruminants and reduce the feed-gain ratio, and meanwhile, due to the improvement of the energy utilization efficiency and the enhancement of the antioxidant ability, the immunity of animals is improved, the morbidity is reduced, and the meat quality is improved.

The application discloses a kind of multifunctional low-temperature degradation corn stalk composite microbial inoculant, preparation method and application, the composite microbial inoculant is compounded by multiple microorganisms with straw degradation, potassium solution and promoting function, comprising the following strains: bacillus subtilis K15, curvularia geniculata L11 and bacillus mycoides L31.The composite microbial inoculant constructed in the application realizes synergistic effect and functional complementation, through strain scientific compounding, straw decomposition rate is significantly improved, and decomposition period is obviously shortened;Enhance the adaptability and colonization competitiveness of microorganisms in winter low temperature and complex soil environment.The composite microbial inoculant of the application also has multiple functions such as straw degradation, potassium solution and IAA production, etc.At the same time of releasing nutrients by rapid decomposition of straw, it can convert difficult-to-use soil potassium into available potassium.

The invention discloses bacillus subtilis S1703 and application thereof, and belongs to the technical field of plant disease and pest control. The name of the bacterial strain is bacillus subtilis S1703, and the preservation number of the bacterial strain is GDMCC No: 66890. The bacterial strain S1703 has a wider antibacterial spectrum, and can effectively inhibit the growth of pathogenic bacteria such as fusarium oxysporum, colletotrichum gloeosporioides, alternaria alternata, aspergillus, phyllosticta, citrus reticulata, fusarium and the like; fermentation liquor of the S1703 has a good antibacterial effect on the psidium guajava wilt pathogen and the psidium guajava branch blight pathogen; the bacteriostasis rate on sigatoka sigatoka and guava leaf spot germs reaches 95% or above, and an excellent bacteriostasis effect is achieved. The strain S1703 can avoid potential adverse effects of chemical pesticides on the environment and crop safety, and has potential commercial development and application values in biological prevention and control practice of diseases.

The invention discloses a biochar-based microbial soil conditioner and a preparation method thereof, and relates to the technical field of soil improvement. The preparation method comprises the following steps: firstly, carrying out alkali-acid treatment and segmented pyrolysis on raw materials such as wood chips to obtain primary biochar, and then carrying out chemical activation, nano material modification and plasma treatment to obtain the composite modified biochar. Meanwhile, probiotics such as bacillus subtilis and the like are subjected to activation, expanding culture and saline-alkali domestication to obtain directional domestication bacterial liquid, and the directional domestication bacterial liquid is solidified into microspheres by adopting a composite embedding carrier. And finally, mixing the composite modified biochar, the composite embedded microspheres, potassium humate and other auxiliary materials, drying, and sieving to obtain the soil conditioner. The soil conditioner disclosed by the invention can realize an improvement effect on saline-alkali soil and reduce the total salt content; meanwhile, directionally domesticated microorganisms secrete organic acid to neutralize alkaline components, the pH of the soil is adjusted to the range suitable for crop growth, and salt and alkali stress is relieved.

The invention provides a pet hair decomposer with synergistic effect of bacillus subtilis and keratinase, the pet hair decomposer comprises bacillus subtilis ZYpet-015 and keratinase, the preservation number of the bacillus subtilis ZYpet-015 is CGMCC (China General Microbiological Culture Collection Center) No.34790, and the preservation date is June 6, 2025; the protease activity of the bacillus subtilis is greater than or equal to 129.92 U, the survival rate of the bacillus subtilis in simulated gastric juice within 3 hours is greater than 50%, and the tolerance rate of the bacillus subtilis in intestinal juice within 4 hours is greater than 90%. According to the pet hair decomposing agent, hair keratin is synergistically degraded through protease secreted by the bacillus subtilis ZYpet-015 and keratinase, and the hair decomposing efficiency reaches 28.07% + / -2.79% and is remarkably higher than that of a single treatment group. Meanwhile, experiments prove that the pet hair decomposer can effectively destroy the hair cuticle structure, is suitable for a pet hair-melting nutritional supplement and can relieve the hair ball symptom.

The application discloses an Escherichia coli probiotic with high beta-alanine yield and application thereof, and the recombinant genetically engineered bacterium takes a wild-type Escherichia coli probiotic as a starting strain and is subjected to one or more gene edits, including Trc promoter strengthening of a panD gene, an aspC gene, an sdhC gene and a ppc gene, overexpression of a panD gene from Bacillus subtilis, an aspB gene from Corynebacterium glutamicum, an aspA gene and a pyc gene from Corynebacterium glutamicum on a plasmid pTrc99a, and knockout of a cycA gene. 600 Compared with an existing reported Escherichia coli probiotic beta-alanine production strain, the OD of the recombinant genetically engineered bacterium is 4.4 times that of the existing reported strain, and the beta-alanine yield is 6 times that of the existing reported strain, so that the recombinant genetically engineered bacterium has great industrial application potential.

The invention provides bacillus subtilis and application thereof, the bacillus subtilis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.29256. The bacterial strain has a good broad-spectrum antibacterial effect, and can be used for preparing the bacillus subtilis. The microbial agent has good inhibition effects on various plant fungal pathogens and bacterial pathogens causing plant diseases, especially has good prevention and treatment effects on highland barley smut, the prevention and treatment effects on the highland barley smut can reach 95% or above by using 10,000,000 cfu / mL of a microbial agent diluent, and the microbial agent has excellent effects.