The invention relates to a
protein glutaminase production strain, a
screening method and a characteristic
analysis method. The invention discloses a
protein glutaminase high-yield strain, which is named as
Chryseobacterium proteolyticumA4142, and is preserved in the China General
Microbiological Culture Collection Center on July 10, 2020, the preservation address is No.3,
Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC (China General
Microbiological Culture Collection Center) NO.20337. The invention further discloses a preparation method of the
protein glutaminase high-yield strain. The
bacterial strain is rod-shaped, does not produce spores, is
gram negative, has a round
bacterial colony, and is full in state, smooth in edge, free of notch and sticky. According to the present invention, the
Chryseobacterium prion A4142 strain is subjected to
fermentation culture in the culture medium, the
enzyme yield is up to 2.15 U / mL, and the
Chryseobacterium prion A4142 is the strain subjected to
food safety certification; the invention further discloses a
screening method of the
novel protein glutaminase high-yield strain, and characteristic analysis is carried out on the
novel protein glutaminase high-yield strain to determine that the strain is Chryseobacterium prion. The method disclosed by the invention has a good implementation prospect in the aspect of industrial production of the protein glutaminase.