15 results about "Chryseobacterium" patented technology

The invention relates to a high-yield protein glutaminase Chryseobacterium prion strain and a preparation method thereof. The strain is obtained by carrying out ARTP and NTG compound mutagenesis treatment on an original strain of Chryseobacterium prion and carrying out high-throughput screening on the strain subjected to the compound mutagenesis treatment. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 20251792. The invention also relates to a method for preparing protein glutaminase by using the strain of the invention. The enzyme activity of the protein glutaminase of the strain is 6 times that of an original strain, and the production efficiency of the protein glutaminase can be remarkably improved.

The invention provides a strain of Chryseobacterium ZJU645, a biological preparation, a culture method, a fermentation method and application, and particularly belongs to the technical field of microbial control. The Chryseobacterium sp. ZJU645 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.35186, and the preservation date is July 10, 2025. The Chryseobacterium ZJU645 disclosed by the invention has a broad-spectrum antifungal effect, and has a relatively high antibacterial effect on alternaria alternata, sclerotinia sclerotiorum, fusarium oxysporum, paniculate corn, colletotrichum gloeosporioides and botrytis cinerea, and a fermentation product of the Chryseobacterium ZJU645 is stable. Through further optimization of fermentation conditions of the Chryseobacterium sp. ZJU645, the disease resistance of the Chryseobacterium sp. ZJU645 is improved, and a new biological control means is provided for more efficient prevention and treatment of agricultural diseases, especially mulberry diseases.

The invention relates to a Chryseobacterium-derived protein glutaminase PG3, a coding gene and an application thereof, and belongs to the technical field of biological genetic engineering. The amino acid sequence of the protein glutaminase PG3 is as shown in SEQ ID No. 1, and the cDNA sequence of the coding gene of the protein glutaminase PG3 is as shown in SEQ ID No. 2. A large amount of protein glutaminase PG3 from Chryseobacterium is prepared through an Escherichia coli expression system, and the protein glutaminase is used for carrying out enzymatic deamidation modification on the spotted silver carp myofibrillar protein PG3, so that the solubility of the spotted silver carp myofibrillar protein under a salt-free system condition is remarkably improved. The invention provides an important tool enzyme for protein deamidation modification, and lays a theoretical foundation for development of low-salt fish protein processed products.

The invention discloses a strain of Chryseobacterium wanjuense C14, and a preparation method of the Chryseobacterium wanjuense C14. The invention further discloses application of the bacillus subtilis in inhibition of ralstonia solanacearum and application of the bacillus subtilis in degradation of straw. The Chryseobacterium sp. C14 can obviously inhibit the growth of pathogenic bacteria of tomato bacterial wilt, especially under the assistance of tomato rhizosphere metabolites such as O-phosphoethanolamine, mangiferin and L-tryptophan, the antibacterial effect of the Chryseobacterium sp. C14 is further enhanced, and the Chryseobacterium sp. C14 has higher environmental adaptability and prevention and treatment potential. The Chryseobacterium sp. C14 can also degrade protein, cellulose and hemicellulose at the same time, and can significantly promote the degradation of rice straw and corn straw. The Chryseobacterium sp. C14 has the functions of straw degradation and tomato ralstonia solanacearum inhibition, integrates a soil-borne disease biological control function and efficient straw degradation, and provides a green and efficient microbial new strategy for solving the problems of difficult straw treatment and difficult tomato bacterial wilt control in agricultural production.

The invention provides application of protein glutaminase to preparation of salt-reduced, fresh-increased and fishy-smell-removed fish protein gel products, and belongs to the field of food processing. A large amount of protein glutaminase PG3 derived from Chryseobacterium is prepared through a heterologous expression technology, the protein glutaminase PG3 is used for carrying out enzymatic deamidation modification on spotted silver carp MP, and finally, a spotted silver carp MP solution obtained after deamidation is heated and refrigerated to prepare the salt-free thermally-induced MP gel. In addition, the contents of substances with fishy smell, such as hexanal, nonanal and 1-octylene-3-alcohol, in the salt-free heat-induced MP gel are remarkably reduced, and the delicate flavor perception is improved. The method not only plays an important role in fishy smell removal and freshness enhancement of the fish protein gel, but also provides scientific basis and technical support for healthy and sustainable development of high-quality salt-reduced MP gel products.

The invention discloses a bacteriophage MA9V-2 and application thereof, the bacteriophage MA9V-2 is classified and named as Chryseobacteria inogenes phage MA9V-2, the preservation number is CCTCC NO: M2024593, and the bacteriophage MA9V-2 is preserved in China Center for Type Culture Collection on April 1, 2024. Wuhan. Wuhan University), the bacteriophage can be used for cracking to produce the chrysobacter indole-3 MA9. Particularly, the bacteriophage MA9V-2 is widely applied to preparation of biological agents and can be used for preparing the biological agents for panax notoginseng bacterial root rot, preparations for producing chrysobacter indoleformans MA9 and preparations for preventing and controlling panax notoginseng root rot, and the biological agents contain the bacteriophage MA9V-2 and pharmaceutically acceptable carriers, so that the bacteriophage MA9V-2 can be applied to preparation of the biological agents for panax notoginseng bacterial root rot. The optimal infection complex number MOI of the bacteriophage MA9V-2 is 0.01, a new efficient biological prevention and treatment means is provided for prevention and treatment of panax notoginseng root rot, the use of chemical agents is expected to be reduced, and healthy development of the panax notoginseng planting industry is guaranteed.

This invention relates to the field of microbial technology, and provides a composite microbial suspension comprising a *Streptococcus pyogenes* R3 suspension and a *Cyclocarya pallida* N4 suspension in a volume ratio of 1-2:1-2; the *Streptococcus pyogenes* R3 classification name is... Herbaspirillum sp. R3 was deposited on September 20, 2023, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M 20231757; the taxonomic name of Chlorella N4 is... Chryseobacterium endophyticum N4 was deposited on March 31, 2025, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M 2025633. The aforementioned composite microbial suspension promotes nitrogen fixation and nitrogen conversion.

This invention belongs to the field of agricultural microbial technology, and discloses a strain of *Chlorella vulgaris* and its application in improving the stability of mushroom residue substrate. The *Chlorella vulgaris* strain is *Chlorella vulgaris* (…). Chryseobacterium The strain JYT19 (sp.) was deposited on February 2, 2026, with accession number CGMCC No. 37640. It is a new strain isolated from coconut coir material. Inoculating this strain into mushroom substrate significantly improves the physical structural stability of the substrate, delays collapse and compaction, and extends its service life. Simultaneously, it has beneficial effects on plant growth and quality improvement. This invention provides an environmentally friendly and efficient microbial technology solution to address the problems of rapid degradation and poor stability of mushroom substrate, and has significant application value in the fields of soilless cultivation in facility agriculture and resource utilization of organic waste.

The invention relates to the technical field of microbial medicines, and particularly discloses application of intestinal golden thread bacteria in preparation of a medicine for preventing or treating peripheral nerve injury. A mouse model proves that the intestinal golden thread bacteria can regulate the injury microenvironment in the peripheral nerve injury repair process, guide nerve axons to extend in the expected direction and reduce uncontrolled axon growth and wrong dominance, so that the structural accuracy of nerve regeneration is improved.

The invention provides a microbial agent for preventing and treating soybean soil-borne diseases and application of the microbial agent, and belongs to the technical field of agricultural biology. The microbial agent is prepared by compounding a bacterial strain RHEr1 (Enterobacter rogengenkampii), a bacterial strain RHCc2 (Chryseobacterium cucumerii), a bacterial strain RHPc03 (Pseudomonas chlororaphis) and a bacterial strain RHBs9 (Bacillus subtilis), and the ratio of viable counts of the bacterial strains is (20 to 28): (22 to 30): (18 to 24): (5 to 9). The microbial agent effectively prevents and treats soybean root rot caused by fusarium, rhizoctonia and the like through multiple antagonism, nutrition competition and an induction system resistance mechanism, has the functions of nitrogen fixation, phosphate solubilization and auxin secretion, and remarkably promotes the growth of soybean plants. The preparation is environment-friendly, has no chemical residue, is not easy to cause drug resistance, and is suitable for green and sustainable soybean production.

This application relates to the field of genetic engineering technology, specifically to a ketone reductase mutant and its uses. The ketone reductase mutant is formed by one or more amino acid mutations in the amino acid sequence of the wild-type ketone reductase shown in SEQ ID NO. 2, which is derived from *Chryseobacterium* sp. When the ketone reductase and its mutant of this invention are used to resolve milobalin intermediates, the chiral purity of the target product is greater than 99.9%. Compared with currently reported methods for synthesizing target products, this invention avoids the use of metal catalysts when using ketone reductase and its mutant for target product synthesis, making the process greener, more environmentally friendly, and reducing costs. Furthermore, this ketone reductase mutant simplifies the resolution process while improving the chiral purity of the target product, resulting in a simple synthesis method.

This invention relates to the field of microbiology, specifically to *Citrus cucumeroides* G1 and its applications; *Citrus cucumeroides* G1 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 32659, and the deposit date is November 15, 2024; the proposed classification name is *Citrus cucumeroides*. Chryseobacterium cucumber The addition of calcium ions enhances the cadmium resistance of *Chlorella vulgaris* G1, increasing the tolerance concentration from 20 mg / L to 120 mg / L, and promotes the co-precipitation of cadmium carbonate and calcium carbonate, thus improving stability. Chicken manure provides nitrogen and organic matter, while the ammoniation effect of *Chlorella vulgaris* G1 blocks the activation effect of chicken manure on cadmium.

The invention discloses Chryseobacterium nepalensis and application thereof, belongs to the technical field of microorganisms, and solves the problem that in the prior art, the carbon monoxide absorption efficiency in a limited space is not ideal enough. The Chryseobacterium nepalense provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Chryseobacterium nepalense is CGMCC (China General Microbiological Culture Collection Center) NO: 33212. According to the invention, the modern biotechnology is fully utilized, the evolutionary bacterial colony with targeting, environment-friendly and green release of CO gas is extracted from the coal mine environment, and the problem of long-term over-limit of CO at the upper corner is solved.

The invention relates to the technical field of microorganisms, and provides a compound microorganism bacterial suspension which comprises a herbaspirillum R3 bacterial suspension and a Chryseobacterium N4 bacterial suspension in a volume ratio of (1-2): (1-2), the classification name of herbapiril R3 is Herbapiril sp. R3, the herbapiril R3 is preserved in the China Center for Type Culture Collection on September 20, 2023, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20231757; the classification name of the Chryseobacterium endophytum N4 is Chryseobacterium endophytum N4, the Chryseobacterium endophytum N4 is preserved in the China Center for Type Culture Collection on March 31, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2025633. The composite microbial suspension has the effect of promoting solid storage of nitrogen and conversion of nitrogen.

The application belongs to the field of agricultural microbial technology, and discloses a golden yellow bacillus strain and application thereof in mushroom residue substrate stability improvement. Chryseobacterium The golden yellow bacillus strain is a golden yellow bacillus (sp.) strain JYT19. The strain is isolated from coconut dregs material, and is preserved on February 2, 2026, with a preservation number of CGMCC No. 37640. Inoculation of the strain into mushroom residue substrate can significantly improve the physical structure stability of the substrate, delay collapse and hardening, and prolong the service life. Meanwhile, the strain has beneficial effects on plant growth and quality improvement. The application provides an environment-friendly and efficient microbial technical solution for solving the problems of fast degradation and poor stability of mushroom residue substrate, and has important application value in the fields of facility agriculture soilless culture and organic waste resource utilization.