26 results about "Chryseobacterium" patented technology

The invention provides a genetically engineered bacterium and a construction method of the genetically engineered bacterium, and the genetically engineered bacterium is characterized in that a green fluorescent protein sfGFP is used for mediating protein glutaminase, PG (protein glutaminase), PGF (protein glutaminase), PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF and PGF; eC 3.5. 1.44) is efficiently secreted and expressed in engineering bacteria, and belongs to the technical field of biological engineering. The method comprises the following steps: an obtained sfGFP gene is derived from an NCBI database (GenBank numbering: CP035486.1), an obtained PG zymogen gene PP (Propeptide-Protein glutaminase) is derived from a Chryseobacterium prion prgA gene (GenBank numbering: AB046594.1) in the NCBI database, a recombinant plasmid pHT01 / sfGFP-PP is introduced into bacillus subtilis WB800N to obtain an engineering bacterium, extracellular secretion expression of PP is realized, the sfGFP-PP is activated by trypsin to obtain PG, and the PG is subjected to enzyme activation to obtain the recombinant bacillus subtilis. The expression quantity and the secretion efficiency of the PG in the bacillus subtilis are greatly improved. The recombinant PG produced by the method can generate 23.5 U / mL enzymatic activity under activation of trypsin, can be used for improving protein functional characteristics, and has relatively high industrial production and application values.

The invention relates to the technical field of environmental microorganisms, in particular to Chryseobacterium Chry.R1 and application of the Chryseobacterium Chry.R1, and the preservation number of the Chryseobacterium Chry.R1 is GDMCC (China General Microbiological Culture Collection Center) No.63568. The Chryseobacterium Chry.R1 has the effects of fixing nitrogen, inhibiting fusarium graminearum and promoting plant growth. The Chryseobacterium Chry.R1 disclosed by the invention can be used for promoting the absorption and utilization of nitrogen elements in a plant growth process, inhibiting the growth of fusarium graminearum and promoting the growth of plants, and a new microbial strain resource is provided for realizing reduction and synergism of chemical fertilizers in agricultural production.

The invention discloses application of cobalamin and beneficial bacteria in prevention and control of tomato soil-borne bacterial wilt. The beneficial bacterium is Chryseobacterium CHR6, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) The invention discloses a synbiotics for preventing and controlling tomato soil-borne bacterial wilt. The synbiotics consist of beneficial bacteria and cobalamin, and the beneficial bacterium is Chryseobacterium CHR6. The invention discloses a method for preventing and controlling tomato soil-borne bacterial wilt by matching cobalamin and beneficial bacteria, which comprises the following steps: 7-10 days after transplanting tomato seedlings, adding synbiotics into soil by a root irrigation method, adding 10-20 mL of cobalamin solution with the concentration of 5-10 nM into each tomato seedling, and inoculating 10-20 mL of beneficial bacteria suspension with the concentration of 1 * 10 < 7 >-1 * 10 < 9 > CFU / mL. By adding cobalamin, inhibition of growth of ralstonia solanacearum through siderophore mediation of Chryseobacterium CHR6 can be remarkably enhanced, and occurrence of tomato soil-borne bacterial wilt is reduced.

The invention relates to the field of microorganisms, in particular to cucumber Chryseobacterium G1 and application thereof. The cucumber Chryseobacterium sp. G1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32659, and the preservation date is November 15, 2024; according to the present invention, the suggested classification name is Chryseobacterium cucumerii, and the preservation number is CGMCC (China General Microbiological Culture Collection Center); by adding calcium ions, the cadmium resistance of the cucumber Chryseobacterium G1 is enhanced, the tolerance concentration is increased from 20 mg / L to 120 mg / L, co-precipitation of cadmium carbonate and calcium carbonate is promoted, and the stability is improved; chicken manure is used for providing a nitrogen source and organic matters, and meanwhile, the activation effect of the chicken manure on cadmium is blocked through the ammoniation effect of cucumber Chryseobacterium G1.

The invention relates to the technical field of phytopathogen prevention and control, and discloses a pogonatum insignis strain Chryseobacteria insignis KL01 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34549. The invention further discloses a preparation method of the pogonatum insignis strain Chryseobacteria insignis strain and application of the pogonatum insignis strain Chryseobacteria insignis strain KL01, and the pogonatum insignis strain Chryseobacteria insignis strain KL01, the pogonatum insignis strain KL01 and the preservation number is CGMCC No.34549. According to the present invention, the mycosphaeria farinoides C.polytrichi KL01 obtained through separation and purification shows the good antagonistic effect on a variety of plant pathogens, such that the mycosphaeria farinoides C.polytrichi KL01 obtained through the present invention can be used for the prevention and control of plant pathogens, and has excellent prevention and control effects; and the mycosphaeria farinoides C.polytrichi KL01 can be used for the prevention and control of the plant pathogens, such that the mycosphaeria farinoides C.polytrichi KL01 can be used for the prevention and control of the plant pathogens.

The invention provides Chryseobacterium P6 for degrading unsymmetrical dimethylhydrazine and a degradation method of the Chryseobacterium P6, the strain is obtained by separating from activated sludge which is domesticated by the unsymmetrical dimethylhydrazine for a long time, and the strain is identified as Bacteroidetes door and Chryseobacterium by 16SrDNA (Deoxyribose Nucleic Acid) gene sequence analysis. After being amplified and cultured for 12 hours by an LB culture medium, the strain can show remarkable degradation activity on unsymmetrical dimethylhydrazine under the conditions that the pH is 7.0 and the temperature is 32 DEG C, and the degradation rate within 48 hours can reach 95% or above. The method is suitable for in-situ remediation of industrial wastewater, polluted soil and waste without additionally adjusting the pH value or adding an oxidizing agent; rapid propagation of the strain can be realized by using a conventional culture medium (such as an LB culture medium), complex equipment or energy input is not needed in the degradation process, and the unit treatment cost is reduced by more than 60% compared with that of a chemical oxidation method; no heavy metal catalyst, high-concentration oxidant or adsorbent is used in the whole process, and secondary pollution risks of bromate, regenerated waste liquid and the like are completely eradicated.

The invention relates to a high-yield protein glutaminase Chryseobacterium prion strain and a preparation method thereof. The strain is obtained by carrying out ARTP and NTG compound mutagenesis treatment on an original strain of Chryseobacterium prion and carrying out high-throughput screening on the strain subjected to the compound mutagenesis treatment. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 20251792. The invention also relates to a method for preparing protein glutaminase by using the strain of the invention. The enzyme activity of the protein glutaminase of the strain is 6 times that of an original strain, and the production efficiency of the protein glutaminase can be remarkably improved.

The present invention relates to the use of a bacterial strain or a combination of bacterial strains in the preparation of a probiotic for fish, said bacterial strains being selected from the following: a Chryseobacterium massiliae strain identified by accession number No. I-5479 deposited at the CNCM on January 24, 2020 and a Flavobacterium sp. strain identified by accession number No. I-5481 deposited at the CNCM on January 24, 2020. The probiotic use includes preventing or minimizing Flavobacterium columnare infection in fish. The present invention also relates to the deposited bacterial strains, or a probiotic composition or kit comprising the same, and a method for identifying a probiotic bacterial strain resistant to pathogen infection.

The invention provides a strain of Chryseobacterium ZJU645, a biological preparation, a culture method, a fermentation method and application, and particularly belongs to the technical field of microbial control. The Chryseobacterium sp. ZJU645 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.35186, and the preservation date is July 10, 2025. The Chryseobacterium ZJU645 disclosed by the invention has a broad-spectrum antifungal effect, and has a relatively high antibacterial effect on alternaria alternata, sclerotinia sclerotiorum, fusarium oxysporum, paniculate corn, colletotrichum gloeosporioides and botrytis cinerea, and a fermentation product of the Chryseobacterium ZJU645 is stable. Through further optimization of fermentation conditions of the Chryseobacterium sp. ZJU645, the disease resistance of the Chryseobacterium sp. ZJU645 is improved, and a new biological control means is provided for more efficient prevention and treatment of agricultural diseases, especially mulberry diseases.

The invention relates to a Chryseobacterium-derived protein glutaminase PG3, a coding gene and an application thereof, and belongs to the technical field of biological genetic engineering. The amino acid sequence of the protein glutaminase PG3 is as shown in SEQ ID No. 1, and the cDNA sequence of the coding gene of the protein glutaminase PG3 is as shown in SEQ ID No. 2. A large amount of protein glutaminase PG3 from Chryseobacterium is prepared through an Escherichia coli expression system, and the protein glutaminase is used for carrying out enzymatic deamidation modification on the spotted silver carp myofibrillar protein PG3, so that the solubility of the spotted silver carp myofibrillar protein under a salt-free system condition is remarkably improved. The invention provides an important tool enzyme for protein deamidation modification, and lays a theoretical foundation for development of low-salt fish protein processed products.

The invention discloses a strain of Chryseobacterium wanjuense C14, and a preparation method of the Chryseobacterium wanjuense C14. The invention further discloses application of the bacillus subtilis in inhibition of ralstonia solanacearum and application of the bacillus subtilis in degradation of straw. The Chryseobacterium sp. C14 can obviously inhibit the growth of pathogenic bacteria of tomato bacterial wilt, especially under the assistance of tomato rhizosphere metabolites such as O-phosphoethanolamine, mangiferin and L-tryptophan, the antibacterial effect of the Chryseobacterium sp. C14 is further enhanced, and the Chryseobacterium sp. C14 has higher environmental adaptability and prevention and treatment potential. The Chryseobacterium sp. C14 can also degrade protein, cellulose and hemicellulose at the same time, and can significantly promote the degradation of rice straw and corn straw. The Chryseobacterium sp. C14 has the functions of straw degradation and tomato ralstonia solanacearum inhibition, integrates a soil-borne disease biological control function and efficient straw degradation, and provides a green and efficient microbial new strategy for solving the problems of difficult straw treatment and difficult tomato bacterial wilt control in agricultural production.

The invention provides application of protein glutaminase to preparation of salt-reduced, fresh-increased and fishy-smell-removed fish protein gel products, and belongs to the field of food processing. A large amount of protein glutaminase PG3 derived from Chryseobacterium is prepared through a heterologous expression technology, the protein glutaminase PG3 is used for carrying out enzymatic deamidation modification on spotted silver carp MP, and finally, a spotted silver carp MP solution obtained after deamidation is heated and refrigerated to prepare the salt-free thermally-induced MP gel. In addition, the contents of substances with fishy smell, such as hexanal, nonanal and 1-octylene-3-alcohol, in the salt-free heat-induced MP gel are remarkably reduced, and the delicate flavor perception is improved. The method not only plays an important role in fishy smell removal and freshness enhancement of the fish protein gel, but also provides scientific basis and technical support for healthy and sustainable development of high-quality salt-reduced MP gel products.

The present invention discloses a novel biological preparation for preventing and treating Panax notoginseng root rot and its application. The main active ingredient of the preparation is Panax notoginseng total saponins. Ginsenoside Rb1 in the Panax notoginseng total saponins inhibits the growth of the Chryseobacterium MA9 strain, which is responsible for Panax notoginseng root rot. This further prevents and treats Panax notoginseng root rot, improves the survival rate of Panax notoginseng seedlings, increases yield, and significantly improves economic benefits. Furthermore, a new approach to preventing and treating Panax notoginseng root rot is provided.

The invention relates to the technical field of experimental animals, in particular to a hatching method of bacteria-limited chickens and application of the bacteria-limited chickens, and the hatching method comprises the following steps: disinfecting the surfaces of SPF hatching eggs by using alcohol with the volume fraction of 60-80% and ultraviolet rays every day during hatching, and disinfecting by using a formaldehyde fumigation method every other week. According to the method provided by the invention, a sterile environment in the hatching process is created through disinfection at a specific time, and the SPF hatching eggs in the hatching process are disinfected, so that the breeding of microorganisms on the surfaces of eggshells is inhibited, the microbial pollution in the exchange process of chick embryos and external environment substances is reduced to a great extent, and the survival rate of the chick embryos is increased. The bacterium-limited chicken with beneficial bacteria such as rumen bacillus, enterococcus, bacillus terminus and proteophilic bacteria is obtained, and no Chryseobacterium exists in the bacterium-limited chicken.

The invention discloses a bacteriophage MA9V-2 and application thereof, the bacteriophage MA9V-2 is classified and named as Chryseobacteria inogenes phage MA9V-2, the preservation number is CCTCC NO: M2024593, and the bacteriophage MA9V-2 is preserved in China Center for Type Culture Collection on April 1, 2024. Wuhan. Wuhan University), the bacteriophage can be used for cracking to produce the chrysobacter indole-3 MA9. Particularly, the bacteriophage MA9V-2 is widely applied to preparation of biological agents and can be used for preparing the biological agents for panax notoginseng bacterial root rot, preparations for producing chrysobacter indoleformans MA9 and preparations for preventing and controlling panax notoginseng root rot, and the biological agents contain the bacteriophage MA9V-2 and pharmaceutically acceptable carriers, so that the bacteriophage MA9V-2 can be applied to preparation of the biological agents for panax notoginseng bacterial root rot. The optimal infection complex number MOI of the bacteriophage MA9V-2 is 0.01, a new efficient biological prevention and treatment means is provided for prevention and treatment of panax notoginseng root rot, the use of chemical agents is expected to be reduced, and healthy development of the panax notoginseng planting industry is guaranteed.

The invention discloses Chryseobacterium sp. HNK6, the preservation number of the Chryseobacterium sp. HNK6 in Guangdong Microbial Culture Collection Center is GDMCC No: 66436, when the Chryseobacterium sp. HNK6 is applied to degradation of chicken feather, the chicken feather can be degraded at the pH of 7-10 and the temperature of 30-40 DEG C, and the degradation rate is 80% or above; the degradation liquid of the Chryseobacterium HNK6 for degrading chicken feathers is used for culturing rhodotorula glutinis, so that the rhodotorula glutinis can efficiently produce the rhodotorula rubra, the production cost of the rhodotorula rubra is reduced, a new way is provided for industrial production of the rhodotorula rubra, and the rhodotorula rubra HNK6 is suitable for industrial production and market popularization and application.

The present invention relates to a bacterial preparation for the treatment of hatchery environment, which contains as an active ingredient a mixture of bacteria Chryseobacterium indologenes and Sphingobacterium multivorum. The invention further relates to the use of said preparation for the preventive improvement of the physiological condition of poultry, in particular for the prevention of infections of poultry by opportunistic pathogens selected from the group comprising E. coli, Clostridium sensu stricto. Enterococcus, Klebsiella, Enterobacter and Pseudomonas.

This invention relates to the field of microbial technology, and provides a composite microbial suspension comprising a *Streptococcus pyogenes* R3 suspension and a *Cyclocarya pallida* N4 suspension in a volume ratio of 1-2:1-2; the *Streptococcus pyogenes* R3 classification name is... Herbaspirillum sp. R3 was deposited on September 20, 2023, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M 20231757; the taxonomic name of Chlorella N4 is... Chryseobacterium endophyticum N4 was deposited on March 31, 2025, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M 2025633. The aforementioned composite microbial suspension promotes nitrogen fixation and nitrogen conversion.

This invention belongs to the field of agricultural microbial technology, and discloses a strain of *Chlorella vulgaris* and its application in improving the stability of mushroom residue substrate. The *Chlorella vulgaris* strain is *Chlorella vulgaris* (…). Chryseobacterium The strain JYT19 (sp.) was deposited on February 2, 2026, with accession number CGMCC No. 37640. It is a new strain isolated from coconut coir material. Inoculating this strain into mushroom substrate significantly improves the physical structural stability of the substrate, delays collapse and compaction, and extends its service life. Simultaneously, it has beneficial effects on plant growth and quality improvement. This invention provides an environmentally friendly and efficient microbial technology solution to address the problems of rapid degradation and poor stability of mushroom substrate, and has significant application value in the fields of soilless cultivation in facility agriculture and resource utilization of organic waste.

The invention relates to the technical field of microbial medicines, and particularly discloses application of intestinal golden thread bacteria in preparation of a medicine for preventing or treating peripheral nerve injury. A mouse model proves that the intestinal golden thread bacteria can regulate the injury microenvironment in the peripheral nerve injury repair process, guide nerve axons to extend in the expected direction and reduce uncontrolled axon growth and wrong dominance, so that the structural accuracy of nerve regeneration is improved.

The invention provides a microbial agent for preventing and treating soybean soil-borne diseases and application of the microbial agent, and belongs to the technical field of agricultural biology. The microbial agent is prepared by compounding a bacterial strain RHEr1 (Enterobacter rogengenkampii), a bacterial strain RHCc2 (Chryseobacterium cucumerii), a bacterial strain RHPc03 (Pseudomonas chlororaphis) and a bacterial strain RHBs9 (Bacillus subtilis), and the ratio of viable counts of the bacterial strains is (20 to 28): (22 to 30): (18 to 24): (5 to 9). The microbial agent effectively prevents and treats soybean root rot caused by fusarium, rhizoctonia and the like through multiple antagonism, nutrition competition and an induction system resistance mechanism, has the functions of nitrogen fixation, phosphate solubilization and auxin secretion, and remarkably promotes the growth of soybean plants. The preparation is environment-friendly, has no chemical residue, is not easy to cause drug resistance, and is suitable for green and sustainable soybean production.

This application relates to the field of genetic engineering technology, specifically to a ketone reductase mutant and its uses. The ketone reductase mutant is formed by one or more amino acid mutations in the amino acid sequence of the wild-type ketone reductase shown in SEQ ID NO. 2, which is derived from *Chryseobacterium* sp. When the ketone reductase and its mutant of this invention are used to resolve milobalin intermediates, the chiral purity of the target product is greater than 99.9%. Compared with currently reported methods for synthesizing target products, this invention avoids the use of metal catalysts when using ketone reductase and its mutant for target product synthesis, making the process greener, more environmentally friendly, and reducing costs. Furthermore, this ketone reductase mutant simplifies the resolution process while improving the chiral purity of the target product, resulting in a simple synthesis method.

This invention relates to the field of microbiology, specifically to *Citrus cucumeroides* G1 and its applications; *Citrus cucumeroides* G1 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 32659, and the deposit date is November 15, 2024; the proposed classification name is *Citrus cucumeroides*. Chryseobacterium cucumber The addition of calcium ions enhances the cadmium resistance of *Chlorella vulgaris* G1, increasing the tolerance concentration from 20 mg / L to 120 mg / L, and promotes the co-precipitation of cadmium carbonate and calcium carbonate, thus improving stability. Chicken manure provides nitrogen and organic matter, while the ammoniation effect of *Chlorella vulgaris* G1 blocks the activation effect of chicken manure on cadmium.

The invention discloses Chryseobacterium nepalensis and application thereof, belongs to the technical field of microorganisms, and solves the problem that in the prior art, the carbon monoxide absorption efficiency in a limited space is not ideal enough. The Chryseobacterium nepalense provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Chryseobacterium nepalense is CGMCC (China General Microbiological Culture Collection Center) NO: 33212. According to the invention, the modern biotechnology is fully utilized, the evolutionary bacterial colony with targeting, environment-friendly and green release of CO gas is extracted from the coal mine environment, and the problem of long-term over-limit of CO at the upper corner is solved.

The invention relates to the technical field of microorganisms, and provides a compound microorganism bacterial suspension which comprises a herbaspirillum R3 bacterial suspension and a Chryseobacterium N4 bacterial suspension in a volume ratio of (1-2): (1-2), the classification name of herbapiril R3 is Herbapiril sp. R3, the herbapiril R3 is preserved in the China Center for Type Culture Collection on September 20, 2023, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20231757; the classification name of the Chryseobacterium endophytum N4 is Chryseobacterium endophytum N4, the Chryseobacterium endophytum N4 is preserved in the China Center for Type Culture Collection on March 31, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2025633. The composite microbial suspension has the effect of promoting solid storage of nitrogen and conversion of nitrogen.

The application belongs to the field of agricultural microbial technology, and discloses a golden yellow bacillus strain and application thereof in mushroom residue substrate stability improvement. Chryseobacterium The golden yellow bacillus strain is a golden yellow bacillus (sp.) strain JYT19. The strain is isolated from coconut dregs material, and is preserved on February 2, 2026, with a preservation number of CGMCC No. 37640. Inoculation of the strain into mushroom residue substrate can significantly improve the physical structure stability of the substrate, delay collapse and hardening, and prolong the service life. Meanwhile, the strain has beneficial effects on plant growth and quality improvement. The application provides an environment-friendly and efficient microbial technical solution for solving the problems of fast degradation and poor stability of mushroom residue substrate, and has important application value in the fields of facility agriculture soilless culture and organic waste resource utilization.