Acquisition method of halohydrocarbon degrading flora suitable for bioelectrochemical repair system

A bioelectrochemical and remediation system technology, applied in the field of groundwater pollution control, can solve the problems of lack of microorganisms that degrade halogenated hydrocarbons, restrict the application of halogenated hydrocarbons, and the application of bioremediation technology for contaminated groundwater, and achieve the purpose of strengthening electrochemical in-situ microorganisms Restoration effect, strong environmental protection, implementation cost and strong environmental protection effect

Inactive Publication Date: 2020-12-08
CHINA UNIV OF GEOSCIENCES (WUHAN)
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Problems solved by technology

At the same time, there may be a lack of microorganisms that degrade halogenated hydrocarbons in the soil, which

Method used

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  • Acquisition method of halohydrocarbon degrading flora suitable for bioelectrochemical repair system
  • Acquisition method of halohydrocarbon degrading flora suitable for bioelectrochemical repair system
  • Acquisition method of halohydrocarbon degrading flora suitable for bioelectrochemical repair system

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Embodiment 1

[0043] A method for obtaining halogenated hydrocarbon-degrading bacterial flora suitable for bioelectrochemical repair systems, which mainly includes the following steps:

[0044] S1. Configure 500ml of inorganic salt culture medium, add 50g of sediment, 0.9g of co-metabolism substrate and 3.4 μ L of trichlorethylene TCE, to obtain the first mixed solution, the cathode sheet and the anode sheet are connected to electricity, and the output current of the DC power supply is set to 20mA, and the magnetic stirrer 20 is turned on to fully carry out the first mixed solution. After stirring and cultivating for 7 days, the first generation of domesticated bacteria liquid is obtained; wherein, the composition of the inorganic salt medium is: KH 2 PO 4 0.53g / L, Na 2 HPO 4 12H 2 O 0.43g / L, MgSO 4 ·7H 2 O 0.037g / L and NaNO 3 0.85g / L, the sediment is contaminated by halogenated hydrocarbons, and the co-metabolism substrate is glucose;

[0045] S2. Adopt the method of TCE gradient...

Embodiment 2

[0050] S1. Configure 750ml of inorganic salt culture medium, add 50g of sediment, 1.44g of co-metabolism substrate and 3.4 μ L of trichlorethylene TCE, to obtain the first mixed solution, the cathode sheet and the anode sheet are connected to electricity, and the output current of the DC power supply is set to 30mA, and the magnetic stirrer 20 is turned on to fully carry out the first mixed solution. After stirring and cultivating for 7 days, the first generation of domesticated bacteria liquid is obtained; wherein, the composition of the inorganic salt medium is: KH 2 PO 4 0.53g / L, Na 2 HPO 4 12H 2 O 0.43g / L, MgSO 4 ·7H 2 O 0.037g / L and NaNO 3 0.85g / L, the sediment is contaminated by halogenated hydrocarbons, and the co-metabolism substrate is glucose;

[0051] S2. Adopt the method of TCE gradient acclimatization to subculture, select a clean incubator 10, and add 500 ml of sterilized inorganic salt medium and 50 ml of the first-generation domesticated bacterium liqu...

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Abstract

The invention relates to the field of groundwater pollution treatment, in particular to an acquisition method of halohydrocarbon degrading flora suitable for a bioelectrochemical repair system. The acquisition method mainly comprises the following steps: constructing an electrochemical bacteria liquid culture device by adopting a culture device, providing O2 and H2 for microorganisms by electrolyzing water, adding an inorganic salt culture medium, a co-metabolism matrix and sediments polluted by halogenated hydrocarbon into to the culture device, and performing subculture by adopting a TCE gradient domestication method with the TCE concentration of 76-608[mu] mol/L in the culture process, so as to finally obtain the halohydrocarbon degrading flora. The main dominant bacteria of the flora comprise Chryseobacterium, Sphingomonas and Microbacterium. The acquisition method of halohydrocarbon degrading flora provides an acquisition method for the flora which can survive in an electrochemical repair system and has certain degradation capacity on halohydrocarbon, and the flora can be used for strengthening the electrochemical in-situ microbial repair effect and has wide engineering application prospects.

Description

technical field [0001] The invention relates to the field of groundwater pollution control, in particular to a method for obtaining a halogenated hydrocarbon degrading bacterial group suitable for a bioelectrochemical repair system. Background technique [0002] Halogenated hydrocarbons are an important class of organic synthesis intermediates, raw materials for many organic synthesis, widely used in various industrial processes, such as the production of plastics, rubber, fibers, silicone resins, adhesives, heat transfer fluids, metal cleaning agents etc., some of which are classified as known or suspected carcinogens. Due to its widespread use and improper treatment methods, it often causes serious pollution to soil and groundwater, and is extremely harmful to the ecological environment and human health. Trichlorethylene (TCE), as a typical representative of halogenated hydrocarbons, has attracted great attention in groundwater remediation. [0003] In this case, groundw...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N13/00C12M1/42C12M1/02C12M1/00C12R1/01
CPCC12N1/36C12N13/00C12M23/38C12M27/02C12M29/20C12M35/02
Inventor 李翠刘慧余锦涛刘珊袁松虎
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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