83 results about "Rhodotorula mucilaginosa" patented technology

The invention discloses a Rhodotorula mucilaginosa strain and application in fermentation production of carotenoid and oils. The main point of the technical scheme involved in the invention lies in that: the Rhodotorula mucilaginosa strain is preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC No.8926. The Rhodotorula mucilaginosa provided by the invention can be used for fermentation production of carotenoid and oils. According to the invention, Rhodotorula mucilaginosa is utilized to produce carotenoid and oils at the same time, multi-round ARTP (atmospheric and room temperature plasma) mutagenesis is carried out on the production strain so as to obtain a high yield mutant strain, thus providing good application prospects and economic benefits for industrial production of carotenoid and oils.

The invention relates to Rhodotorula mucilaginosa capable of degrading urethane and its application in liquor products and food, which belongs to the biology engineering technical field. The Ethylcarbamate is called EC for short and Urethane. The invention provides a yeast strain capable of degrading urethane, the classification name is Rhodotorula mucilaginosa which is preserved at the common microorganisms center of the CCCCM with the preservation number of 5081. The strain is from Daqu brewed by Chinese liquor, Chinese rice wine wheat Qu, healthy mice manure and gavage EC mice manure, the strain grows by taking EC as sole carbon source, and the EC is finally decomposed to NH4<+>, C2H5OH and CO2 which are no toxic. The Rhodotorula mucilaginosa can be used for brewing the wine, liquor, prepared wine and other food, and is capable of reducing the EC content and food security.

The invention discloses rhodotorula mucilaginosa and a fermentation culture substance and application thereof. The rhodotorula mucilaginosa is rhodotorula mucilaginosa TZR<2014>, and the preservation number is CCTCC NO:M2015574. Liquid state fermentation is conducted on the rhodotorula mucilaginosa by means of the fermentation culture medium, the biomass of fermentation liquor is 18 g/L, and the yield of carotenoid is 36 mg/L. The rhodotorula mucilaginosa has the effects of resisting staphylococcus aureus, gastric acid and cholate, and a solid fermentation substance of the rhodotorula mucilaginosa further contains pyruvic acid which has the functions of accelerating fat consumption, increasing the muscle yield, phagocytosing free radicals in bodies and inhibiting generation of the free radicals. Therefore, the rhodotorula mucilaginosa can be used for feed additives of livestock, poultry and aquatic animals and has a wide application prospect.

The present invention relates to marine microbe applying technology and sea cucumber cultivating technology, and is especially process of producing marine rhodotorula mucilaginosa for sea cucumber. The conventional marine rhodotorula mucilaginosa is cultured in aged sea water with glucose in 1.0-3.8 wt% and EDTA-Fe in 1-3 ml/l at 15-25 deg.c, and the production process includes two stages of fermentation, centrifugal concentration and other steps. The production is simple, short in production period and low in cost, and the marine rhodotorula mucilaginosa product has high quality. The produced marine rhodotorula mucilaginosa is applied in raising sea cucumber larva to raise the metamorphosis rate and survival rate of sea cucumber larva, promote the growth of the larva and improve the ecological environment in the culture pond.

The invention discloses a preparation method of a nano-silver composite antibacterial agent. The invention is characterized in that the nano-silver composite antibacterial agent is formed by combining a nano-silver solution and an organic antibacterial agent which have synergistic effect and adduction effect according to a certain ratio. The structure and performance of the antibacterial agent after combination are stable, and the antibacterial agent has more rapid, efficient and broad-spectrum antibacterial performance. The minimal inhibitory concentration (MIC) of the antibacterial agent for inhibiting escherichia coli, Enterobacter hormaechei, staphylococcus aureus, bacillus subtilis, Rhodotorula mucilaginosa, penicillium and Aspergillus niger is only 1-10 microgram/ml. The preparation method of the nano-silver composite antibacterial agent is characterized in that the nano-silver in the nano-silver composite antibacterial agent has monodispersity and there is no agglomeration phenomenon. The preparation method has advantages of ingenious conception, simple experimental operation, high operationality and short period, and is suitable for large-scale production.

The invention discloses a method for improving the fermentation productivity of carotenoid, which is realized by the following steps: rhodotorula mucilaginosa is used as fermentation strains of the carotenoid, and the rhodotorula mucilaginosa is inoculated into a culture medium according to the inoculum size of 2%-10%; a ferrous ion solvent is added into the culture medium, wherein the volume of addition of the ferrous ion solvent is that the molarity relative to the culture medium is between 0.05-0.5mmol/L, and the fermentation temperature is kept at 27-33 DEG C; after the fermentation lasts for 32-40 hours, the temperature is lowered to 20-25 DEG C for continuous fermentation; the fermentation lasts for 24-46 hours. By adding ferrous ions, the capability of beta-carotene for converting into the high antioxygen carotenoid is greatly improved, the insufficience of quantity of beta-carotene dioxygen dismutase and beta-carotene hydrogenase is made up; the beta-carotene is converted into the high antioxygen carotenoid; by the method, the fermentation yield is improved by 30-34%.

The invention discloses Rhodotorula mucilaginosa which has efficacies of prevention and cure for postharvest disease of fruit, and application and use method thereof in storage and refreshment of fruits. The strain of the invention is CGMCC No.3617. When in use, Rhodotorula mucilaginosa is activated, cultured through fermentation and centrifuged to obtain thalluses, the thalluses are diluted by sterile water to prepare into bacterial suspension of 1.0*107-numbered thalluses/ml to 1.0*109-numbered thalluses/ml. Fruits are put into the bacterial suspension to soak for about 30s to 2min, then taken out immediately to perform air drying, put in a plastic crate, sealed by a preservative film, and then stored under room temperature or in cold storage. In the invention, antagonistic yeast is used to prevent and cure postharvest disease of fruit, the invention has simple use, convenient operation, good effect and low cost, thereby avoiding dangers to people or pollution to environment from using chemical bactericide, thus the invention has significant economic benefits and social benefits.

The invention relates to the field of microorganisms, in particular relates to a heavy metal-resistant glyphosate degrading bacterium (Rhodotorula mucilaginosa), OP11, preservation unit: China General Microbiological Culture Collection Center (CGMCC), preservation date: January 6, 2017, preservation number: CGMCC No. 13540. The heavy metal-resistant glyphosate degrading bacterium has the ability to use glyphosate as carbon and nitrogen source, has the ability to resist heavy metals, can rapidly absorb heavy metals, decompose and assimilate the glyphosate at the same time, and achieve the purpose of reducing the glyphosate in the environment.

The invention discloses a rhodotorula mucilaginosa with metabolism property and an application thereof in biodegradation, belonging to the bio-engineering technical field. The invention separates and filters a rhodotorula mucilaginosa with the metabolism property. The invention is characterized in that: the rhodotorula mucilaginosa degrades nitrobenzene through partial reduction, and mineralizes a subsidiary product, namely pyridine carboxylic acid produced in degradation process, thereby realizing complete degradation of the nitrobenzene, and solving the bottleneck of partial degrading reduction of the nitrobenzene; the rhodotorula mucilaginosa can effectively degrade the nitrobenzene under the condition of high salinity and coexistence of a plurality of organic substances; the rhodotorula mucilaginosa can grow by taking the pyridine carboxylic acid as the only carbon, nitrogen and energy, and the pyridine carboxylic acid is finally mineralized into harmless carbon dioxide and water; and the rhodotorula mucilaginosa can act as a biological strengthening agent to be applied to the biological treatment of nitrobenzene waste water and pyridine carboxylic acid waste water with high salinity and complex compositions.

The invention discloses a Rhodotorula mucilaginosa and the application thereof in the degradation and decoloring of dyes and the production of carotenoid. The Preservation No. of the Rhodotorula mucilaginosa JM401 is CCTCC NO: M 2013088; the saccharomycete can be used for carrying out degradation and decoloring on a triphenlmethane dye: and centrifugally collecting activated seed solution bacteria and vaccinating the bacteria to a decoloring medium containing the dye, and carrying out shake cultivation on the obtained product for 2-12 h at a temperature of 25-37 DEG C and a pH value of 4.0-7.5 so as to obtain a decoloring culture solution; and the saccharomycete can be used for producing carotenoids by fermentation: taking an activated seed solution and vaccinating the activated seed solution to a fermentation medium, and carrying out fermentation cultivation on the obtained product for 36-48 h at a temperature of 25-32 DEG C and a pH value of 5.0-7.0 so as to obtain fermentation liquor containing the carotenoids. The Rhodotorula mucilaginosa disclosed by the invention simultaneously has the abilities of carrying out efficient decoloring on triphenlmethane dyes and carrying out high-yield production on carotenoids, therefore, the Rhodotorula mucilaginosa has an extremely broad application prospect in a field to which the Rhodotorula mucilaginosa belongs.

The invention discloses a compound feed for promoting digestion and growth of trachinotus ovatus. The compound feed for promoting the digestion and growth of the trachinotus ovatus comprises the following components in percentage by weight: 27-32% of white fish meal, 14-22% of soybean protein concentrate, 7-9% of soybean meal, 4-7% of peanut meal, 23-27% of flour, 5.5-7% of soybean oil, 3-8% of fish oil, 0.85-1.2% of calcium dihydrogen phoshate, 0.85-1.0% of vitamin premix, 0.8-0.9% of mineral substance premix, 0.35-0.55% of choline chloride, 0.3-0.7% of lecithin and 0.03-0.04% of rhodotorula mucilaginosa. The compound feed for promoting the digestion and growth of the trachinotus ovatus has the advantages that food calling property is good, the nutrition is balanced, and the growth of the trachinotus ovatus can be obviously promoted; after feeding, the protein content of a trachinotus ovatus body is increased, the activeness of digestive ferments in liver and intestinal tracts is obviously enhanced, the digestion of protein and starch and the absorption and the utilization of the feed are promoted, and the economic benefit of breeding and ecological benefit of the environment can be increased.

The invention relates to the technical field of microorganisms and particularly relates to a bacillus amyloliquefaciens. The bacillus amyloliquefaciens is separated and bred from soil of Jiangxi Fuzhou domestic refuse landfill site; the strain number is SQ-001; the Latin name is bacillus amyloliquefaciens; the strain SQ-001 is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 30, 2016; the preservation address is Building 3, Yard 1, West Beichen Street, Chaoyang District, Beijing; the postcode is 100101; the strain preservation number is CGMCC No.12917. The bacillus amyloliquefaciens strain SQ-001, rhodotorula mucilaginosa CGMCC No. 2.4008, enterococcus faecalis CGMCC No. 1.2024 and acetobacter pasteurianus CGMCC No. 1.2830 are prepared into a composite microbial deodorant through processes of purebred amplification culture and mixed fermentation; the composite microbial deodorant is excellent in deodorization effect and wide in application range.

The invention discloses a rhodotorula mucilaginosa mutant strain and application thereof. The rhodotorula mucilaginosa mutant strain disclosed by the invention is Rhodotorula mucilaginosa ZTHY2 and ispreserved in China Center for Type Culture Collection on May 13th, 2015 with the preservation number of CCTCC NO:M 2015296. The mutant strain is obtained by adopting a compound mutation method, yieldof carotenoid can reach 39mg/L within 72 hours and is 3.6 times of an original strain, the yield can reach 52mg/L after fermentation is carried out for 96 hours, and the rhodotorula mucilaginosa mutant strain has relatively high application value in preparation of an animal growth promoter or an animal feed additive, especially in aspects of improving production performance of sanhuang chicken and weaning piglets, improving quality of eggs and lowering content of cholesterol in egg yolk. Besides, the mutant strain disclosed by the invention is stable in performance, easy to culture, high in breeding speed and high in mycelium biomass and is obviously better than a Rhodotorula strain directly screened from nature.

The invention provides a composite passivator for reducing the heavy metal content of a pig manure organic fertilizer and application of the composite passivator, and belongs to the field of organic fertilizers. The composite passivator for reducing the heavy metal content of the pig manure organic fertilizer comprises the following components by weight: 50-70 parts of thiourea modified peanut shell charcoal, 30-50 parts of a heavy metal passivating fungicide, 20-30 parts of crab shell powder, 10-20 parts of fulvic acid, and 3-5 parts of nanometer carbon black; every 100 mg of an auxiliary material of the heavy metal passivating fungicide contains 2.25*10<7> to 2.5*10<8> CFU of rhodotorula mucilaginosa strain OP11; and the preservation number of the rhodotorula mucilaginosa strain OP11 isCGMCC No.13540. The invention also discloses the application of the composite passivator to reducing the heavy metal content of the pig manure organic fertilizer.

The invention discloses a composite microecological preparation and an application thereof. The composite microecological preparation comprises saccharomycetes, acid-producing bacteria and bacillus, wherein the viable count ratio of the saccharomycetes to the acid-producing bacteria to the bacillus is (1-3): (1-4): (1-2), the effective viable count of each gram of the composite microecological preparation is greater than or equal to 1010 CFU/g, the saccharomycetes are selected from at least one of saccharomyces cerevisiae, phaffia rhodozyma, rhodotorula mucilaginosa and candida utilis, the acid-producing bacteria are selected from at least one of lactobacillus plantarum, enterococcus faecalis, enterococcus faecium, clostridium butyricum and lactobacillus rhamnosus, and the bacillus is selected from at least one of bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus amyloliquefaciens and bacillus megatherium. According to the composite microecological preparation and the application thereof, the intestinal health level of animals can be improved, the growth of the animals can be promoted, the anti-stress capability of the animals can be improved, the immune system of the animals can be activated, and diseases can be reduced.

The invention discloses a method for enhancing mussel astaxanthin fermentation production yield, which comprises the following steps: by using rhodotorula mucilaginosa as a fermentation strain, inoculating 2-10% of the rhodotorula mucilaginosa into a culture medium, and adding calcium carbonate and magnesium sulfate into the culture medium, wherein the addition amount of the calcium carbonate is 0.1-0.5 g/L, and the magnesium sulfate is magnesium sulfate heptahydrate with the addition amount of 0.1-0.7 g/L; and fermenting in a 5L fermentation tank for 3 days while regulating the ventilation quantity and rotation speed of the fermentation tank and keeping the fermentation temperature at 25-33 DEG C and the pH value at 5.5-7.5. On the premise of ensuring the product yield, the method can lower the energy consumption and reduce the fermentation operating cost by controlling the ventilation quantity and rotation speed in different fermentation stages, and thus, has important meanings for reducing industrialized production cost.

A Rhodotorula mucilaginosa strain ZZR-1# is named as Rhodotorula mucilaginosa, is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No.8890. The method using Rhodotorula mucilaginosa ZZR-1# to produce cutinase comprises the following steps: carrying out slant culturing on the Rhodotorula mucilaginosa by a slant culture medium, carrying out shaking seed culture, enlarged culture, enlarged culture and fermentation tank culture, filtering the obtained fermentation liquid by a gauze, and centrifuging the obtained filtrate at 4DEG C under a rotating speed of 5500rpm for 30min to obtain a supernatant which is a crude cutinase liquid; and concentrating the crude cutinase liquid, and purifying to obtain cutinase. The method using the Rhodotorula mucilaginosa ZZR-1# to produce cutinase is designed against the characteristics of the strain, and has the advantages of reasonability, simplicity, low cost, high enzyme activity in the crude cutinase liquid, high cutinase output, and good application prospect.

The present invention refers to a process for obtaining copper nanoparticles from Rhodotorula mucilaginosa.

The present invention refers to the use of dead biomass of Rhodotorula mucilaginosa to perform bioremediation of wastewater and for industrial scale production of copper nanoparticles.

In the present invention, it is developed a synthetic strategy for the biosynthesis and removal of copper nanoparticles which is fast, low cost, environment friendly and easily scalable, using as a reduction agent the yeast Rhodotorula mucilaginosa.

The invention discloses a probiotics microecological preparation and a preparation method and an application thereof. The probiotics microecological preparation comprises lactococcus lactis, bacillusamyloliquefaciens and rhodotorula mucilaginosa. The preparation method comprises the following steps: S1, preparing liquid preparations of strains; mixing the liquid preparations of the strains with bran in a weight ratio of 1: (4-11), airing the mixture at 40-60 DEG C, and crushing the mixture to obtain the solid preparations of the strains; and S2, preparation of the probiotics microecological preparation: uniformly mixing the liquid preparations of the strains to obtain probiotics microecological liquid preparations; and uniformly mixing the probiotics microecological liquid preparations toobtain the probiotics microecological preparation. The microecological preparation prepared by the invention is remarkable in synergic effect. The probiotics in the preparation are high in activity and rich in metabolite, the production performance of livestock in different stages can be improved, and the probiotics microecological preparation can be used as a feed additive and a substituent of antibiotics.

The invention discloses Tibet rhodotorula mucilaginosa YC1 and an application of the Tibet rhodotorula mucilaginosa YC1 to low-temperature storage of crystal pears, and belongs to the field of rhodotorula mucilaginosa. The preserving number of the Tibet rhodotorula mucilaginosa YC1 and the application is CGMCC No.10223. The Tibet rhodotorula mucilaginosa YC1 can be used for low-temperature disease control and low-temperature storage and freshness keeping of the crystal pears. An application method includes the specific steps that the rhodotorula mucilaginosa with the preserving number CGMCC No.10223 is activated and cultured in a fermentation mode, and obtained thalli are diluted through sterile water to prepare thallus suspension with the concentration of 1.0*10<7> CFU/ml to 1.0*10<9> CFU/ml; the crystal pears are soaked into the thallus suspension for 1 minute to 2 minutes, taken out, aired, placed into a sealed container and stored under the refrigeration condition of 3 DEG C to 5 DEG C, wherein the storage time can be 30 days.