83 results about "Rhodotorula mucilaginosa" patented technology

The invention provides a composite repair agent for soil heavy metal pollution as well as a preparation method and application thereof, and belongs to the technical field of soil improvement and environmental protection. A composite repair agent for soil cadmium pollution is prepared from the following components in parts by weight: 10 to 30 parts of sodium bentonite, 5 to 15 parts of bone powder,5 to 25 parts of chitosan, 5 to 30 parts of oyster shell powder, 10 to 40 parts of graphene-modified crop straw biochar, 10 to 30 parts of bio-organic fertilizer, 15 to 45 parts of sodium silicate, 1to 15 parts of nano-FeO, 1 to 8 parts of carbon nanotubes, and 1 to 10 parts of microbial inoculants. The number of each 10 mg colonies of rhodotorula mucilaginosa OP11 in the microbial inoculum is 2.25*10 <8> to 2.5*10<9> CFU; the preservation number of the rhodotorula mucilaginosa OP11 is CGMCC No. 13540. The application of the composite repair agent is in remediation of heavy metal contaminated soil.

The invention discloses a Rhodotorula mucilaginosa strain and application in fermentation production of carotenoid and oils. The main point of the technical scheme involved in the invention lies in that: the Rhodotorula mucilaginosa strain is preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC No.8926. The Rhodotorula mucilaginosa provided by the invention can be used for fermentation production of carotenoid and oils. According to the invention, Rhodotorula mucilaginosa is utilized to produce carotenoid and oils at the same time, multi-round ARTP (atmospheric and room temperature plasma) mutagenesis is carried out on the production strain so as to obtain a high yield mutant strain, thus providing good application prospects and economic benefits for industrial production of carotenoid and oils.

The invention relates to Rhodotorula mucilaginosa capable of degrading urethane and its application in liquor products and food, which belongs to the biology engineering technical field. The Ethylcarbamate is called EC for short and Urethane. The invention provides a yeast strain capable of degrading urethane, the classification name is Rhodotorula mucilaginosa which is preserved at the common microorganisms center of the CCCCM with the preservation number of 5081. The strain is from Daqu brewed by Chinese liquor, Chinese rice wine wheat Qu, healthy mice manure and gavage EC mice manure, the strain grows by taking EC as sole carbon source, and the EC is finally decomposed to NH4<+>, C2H5OH and CO2 which are no toxic. The Rhodotorula mucilaginosa can be used for brewing the wine, liquor, prepared wine and other food, and is capable of reducing the EC content and food security.

The invention discloses rhodotorula mucilaginosa and a fermentation culture substance and application thereof. The rhodotorula mucilaginosa is rhodotorula mucilaginosa TZR<2014>, and the preservation number is CCTCC NO:M2015574. Liquid state fermentation is conducted on the rhodotorula mucilaginosa by means of the fermentation culture medium, the biomass of fermentation liquor is 18 g / L, and the yield of carotenoid is 36 mg / L. The rhodotorula mucilaginosa has the effects of resisting staphylococcus aureus, gastric acid and cholate, and a solid fermentation substance of the rhodotorula mucilaginosa further contains pyruvic acid which has the functions of accelerating fat consumption, increasing the muscle yield, phagocytosing free radicals in bodies and inhibiting generation of the free radicals. Therefore, the rhodotorula mucilaginosa can be used for feed additives of livestock, poultry and aquatic animals and has a wide application prospect.

The present invention relates to marine microbe applying technology and sea cucumber cultivating technology, and is especially process of producing marine rhodotorula mucilaginosa for sea cucumber. The conventional marine rhodotorula mucilaginosa is cultured in aged sea water with glucose in 1.0-3.8 wt% and EDTA-Fe in 1-3 ml / l at 15-25 deg.c, and the production process includes two stages of fermentation, centrifugal concentration and other steps. The production is simple, short in production period and low in cost, and the marine rhodotorula mucilaginosa product has high quality. The produced marine rhodotorula mucilaginosa is applied in raising sea cucumber larva to raise the metamorphosis rate and survival rate of sea cucumber larva, promote the growth of the larva and improve the ecological environment in the culture pond.

The invention discloses a preparation method of a nano-silver composite antibacterial agent. The invention is characterized in that the nano-silver composite antibacterial agent is formed by combining a nano-silver solution and an organic antibacterial agent which have synergistic effect and adduction effect according to a certain ratio. The structure and performance of the antibacterial agent after combination are stable, and the antibacterial agent has more rapid, efficient and broad-spectrum antibacterial performance. The minimal inhibitory concentration (MIC) of the antibacterial agent for inhibiting escherichia coli, Enterobacter hormaechei, staphylococcus aureus, bacillus subtilis, Rhodotorula mucilaginosa, penicillium and Aspergillus niger is only 1-10 microgram / ml. The preparation method of the nano-silver composite antibacterial agent is characterized in that the nano-silver in the nano-silver composite antibacterial agent has monodispersity and there is no agglomeration phenomenon. The preparation method has advantages of ingenious conception, simple experimental operation, high operationality and short period, and is suitable for large-scale production.

The invention discloses a method for improving the fermentation productivity of carotenoid, which is realized by the following steps: rhodotorula mucilaginosa is used as fermentation strains of the carotenoid, and the rhodotorula mucilaginosa is inoculated into a culture medium according to the inoculum size of 2%-10%; a ferrous ion solvent is added into the culture medium, wherein the volume of addition of the ferrous ion solvent is that the molarity relative to the culture medium is between 0.05-0.5mmol / L, and the fermentation temperature is kept at 27-33 DEG C; after the fermentation lasts for 32-40 hours, the temperature is lowered to 20-25 DEG C for continuous fermentation; the fermentation lasts for 24-46 hours. By adding ferrous ions, the capability of beta-carotene for converting into the high antioxygen carotenoid is greatly improved, the insufficience of quantity of beta-carotene dioxygen dismutase and beta-carotene hydrogenase is made up; the beta-carotene is converted into the high antioxygen carotenoid; by the method, the fermentation yield is improved by 30-34%.

The invention discloses Rhodotorula mucilaginosa which has efficacies of prevention and cure for postharvest disease of fruit, and application and use method thereof in storage and refreshment of fruits. The strain of the invention is CGMCC No.3617. When in use, Rhodotorula mucilaginosa is activated, cultured through fermentation and centrifuged to obtain thalluses, the thalluses are diluted by sterile water to prepare into bacterial suspension of 1.0*107-numbered thalluses / ml to 1.0*109-numbered thalluses / ml. Fruits are put into the bacterial suspension to soak for about 30s to 2min, then taken out immediately to perform air drying, put in a plastic crate, sealed by a preservative film, and then stored under room temperature or in cold storage. In the invention, antagonistic yeast is used to prevent and cure postharvest disease of fruit, the invention has simple use, convenient operation, good effect and low cost, thereby avoiding dangers to people or pollution to environment from using chemical bactericide, thus the invention has significant economic benefits and social benefits.

The invention relates to the field of microorganisms, in particular relates to a heavy metal-resistant glyphosate degrading bacterium (Rhodotorula mucilaginosa), OP11, preservation unit: China General Microbiological Culture Collection Center (CGMCC), preservation date: January 6, 2017, preservation number: CGMCC No. 13540. The heavy metal-resistant glyphosate degrading bacterium has the ability to use glyphosate as carbon and nitrogen source, has the ability to resist heavy metals, can rapidly absorb heavy metals, decompose and assimilate the glyphosate at the same time, and achieve the purpose of reducing the glyphosate in the environment.

The invention discloses a rhodotorula mucilaginosa with metabolism property and an application thereof in biodegradation, belonging to the bio-engineering technical field. The invention separates and filters a rhodotorula mucilaginosa with the metabolism property. The invention is characterized in that: the rhodotorula mucilaginosa degrades nitrobenzene through partial reduction, and mineralizes a subsidiary product, namely pyridine carboxylic acid produced in degradation process, thereby realizing complete degradation of the nitrobenzene, and solving the bottleneck of partial degrading reduction of the nitrobenzene; the rhodotorula mucilaginosa can effectively degrade the nitrobenzene under the condition of high salinity and coexistence of a plurality of organic substances; the rhodotorula mucilaginosa can grow by taking the pyridine carboxylic acid as the only carbon, nitrogen and energy, and the pyridine carboxylic acid is finally mineralized into harmless carbon dioxide and water; and the rhodotorula mucilaginosa can act as a biological strengthening agent to be applied to the biological treatment of nitrobenzene waste water and pyridine carboxylic acid waste water with high salinity and complex compositions.

The invention relates to rhodotorula mucilaginosa DJHN070401 with high antimony resistance as well as a separation screening method and application thereof, and belongs to the technical field of microorganisms. A strain of the rhodotorula mucilaginosa is classified and named as rhodotorula mucilaginosa DJHN070401, and is preserved in China Center For Type Culture Collection (CCTCC), wherein the preservation number is CCTCC NO: M2018212, and the preservation date is April, 18th, 2018. The strain of the rhodotorula mucilaginosa disclosed by the invention is obtained through performing separationscreening agricultural soil near to northern ore smelting plants of a tin mine in Hunan province. The rhodotorula mucilaginosa DJHN070401 disclosed by the invention has higher adsorption volume and high adsorption speed on antimony ions in a water body, is environmentally friendly, has good application prospect, and has significance on development of a technology for performing adsorption removalon antimony based on microorganisms.

The invention discloses a Rhodotorula mucilaginosa and the application thereof in the degradation and decoloring of dyes and the production of carotenoid. The Preservation No. of the Rhodotorula mucilaginosa JM401 is CCTCC NO: M 2013088; the saccharomycete can be used for carrying out degradation and decoloring on a triphenlmethane dye: and centrifugally collecting activated seed solution bacteria and vaccinating the bacteria to a decoloring medium containing the dye, and carrying out shake cultivation on the obtained product for 2-12 h at a temperature of 25-37 DEG C and a pH value of 4.0-7.5 so as to obtain a decoloring culture solution; and the saccharomycete can be used for producing carotenoids by fermentation: taking an activated seed solution and vaccinating the activated seed solution to a fermentation medium, and carrying out fermentation cultivation on the obtained product for 36-48 h at a temperature of 25-32 DEG C and a pH value of 5.0-7.0 so as to obtain fermentation liquor containing the carotenoids. The Rhodotorula mucilaginosa disclosed by the invention simultaneously has the abilities of carrying out efficient decoloring on triphenlmethane dyes and carrying out high-yield production on carotenoids, therefore, the Rhodotorula mucilaginosa has an extremely broad application prospect in a field to which the Rhodotorula mucilaginosa belongs.

The invention provides a method for preparing a compound microorganism fermented forage feed. The method is characterized by firstly preparing a compound microorganism fermentation agent lyophilized powder of trichoderma koningii CICC 13037, rhodotorula mucilaginosa CICC 31192, bacillus subtilis CICC 10063 and bacillus subtilis CICC 10089, activating the lyophilized powder with 2% of glucose solution and then inoculating the activated lyophilized powder into the fresh forage section, then carrying out insulation fermentation and aeration-drying on the forage and then packaging the forage, thus obtaining the finished product.

The invention discloses a method for controlling apple patulin, belonging to a method for controlling plant postharvest diseases. The method provided by the invention comprises the following steps of: activating rhodotorula mucilaginosa, inoculating the rhodotorula mucilaginosa into an NYDB (Nutrient Yeast Dextrose Broth) culture medium to be cultured and centrifuging to obtain thalli; diluting the thalli by utilizing a 0.2% phytic acid solution to prepare a thalli suspension solution with the concentration of 1*10 to the eighth power per milliliter; adding 30 milliliters of microzyme suspension solution into the wounds of the fruit and naturally drying; and then putting the fruit into a plastic basket and sealing the plastic basket by a preservative film. In the method disclosed by the invention, the phytic acid and the rhodotorula mucilaginosa are combined to control the apple patulin; and the method is environmental-friendly, safe, economical, efficient and practical and has important social values and economic values.

The invention provides a salt-tolerant rhodotorula mucilaginosa strain with high yield of phenylethanol and application thereof. The strain was deposited in the China General Microbiological Culture Collection Center on November 26, 2019 under a deposit number of CGMCC No. 19008. The strain has characteristics of salt tolerance, better growth stability, and producing strong sauce and mellow flavor, significantly increases content of active ingredients in volatile substances such as phenylethyl alcohol, furanone, maltol, 2,6-dimethylpyrazine, etc., and can be used for fermentation of high-saltdilute soy sauce to improve the flavor and quality of soy sauce products.

The invention discloses a compound feed for promoting digestion and growth of trachinotus ovatus. The compound feed for promoting the digestion and growth of the trachinotus ovatus comprises the following components in percentage by weight: 27-32% of white fish meal, 14-22% of soybean protein concentrate, 7-9% of soybean meal, 4-7% of peanut meal, 23-27% of flour, 5.5-7% of soybean oil, 3-8% of fish oil, 0.85-1.2% of calcium dihydrogen phoshate, 0.85-1.0% of vitamin premix, 0.8-0.9% of mineral substance premix, 0.35-0.55% of choline chloride, 0.3-0.7% of lecithin and 0.03-0.04% of rhodotorula mucilaginosa. The compound feed for promoting the digestion and growth of the trachinotus ovatus has the advantages that food calling property is good, the nutrition is balanced, and the growth of the trachinotus ovatus can be obviously promoted; after feeding, the protein content of a trachinotus ovatus body is increased, the activeness of digestive ferments in liver and intestinal tracts is obviously enhanced, the digestion of protein and starch and the absorption and the utilization of the feed are promoted, and the economic benefit of breeding and ecological benefit of the environment can be increased.

The invention relates to the technical field of microorganisms and particularly relates to a bacillus amyloliquefaciens. The bacillus amyloliquefaciens is separated and bred from soil of Jiangxi Fuzhou domestic refuse landfill site; the strain number is SQ-001; the Latin name is bacillus amyloliquefaciens; the strain SQ-001 is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 30, 2016; the preservation address is Building 3, Yard 1, West Beichen Street, Chaoyang District, Beijing; the postcode is 100101; the strain preservation number is CGMCC No.12917. The bacillus amyloliquefaciens strain SQ-001, rhodotorula mucilaginosa CGMCC No. 2.4008, enterococcus faecalis CGMCC No. 1.2024 and acetobacter pasteurianus CGMCC No. 1.2830 are prepared into a composite microbial deodorant through processes of purebred amplification culture and mixed fermentation; the composite microbial deodorant is excellent in deodorization effect and wide in application range.

The invention discloses a rhodotorula mucilaginosa mutant strain and application thereof. The rhodotorula mucilaginosa mutant strain disclosed by the invention is Rhodotorula mucilaginosa ZTHY2 and ispreserved in China Center for Type Culture Collection on May 13th, 2015 with the preservation number of CCTCC NO:M 2015296. The mutant strain is obtained by adopting a compound mutation method, yieldof carotenoid can reach 39mg / L within 72 hours and is 3.6 times of an original strain, the yield can reach 52mg / L after fermentation is carried out for 96 hours, and the rhodotorula mucilaginosa mutant strain has relatively high application value in preparation of an animal growth promoter or an animal feed additive, especially in aspects of improving production performance of sanhuang chicken and weaning piglets, improving quality of eggs and lowering content of cholesterol in egg yolk. Besides, the mutant strain disclosed by the invention is stable in performance, easy to culture, high in breeding speed and high in mycelium biomass and is obviously better than a Rhodotorula strain directly screened from nature.

The invention discloses a composite microecological preparation and an application thereof. The composite microecological preparation comprises saccharomycetes, acid-producing bacteria and bacillus, wherein the viable count ratio of the saccharomycetes to the acid-producing bacteria to the bacillus is (1-3): (1-4): (1-2), the effective viable count of each gram of the composite microecological preparation is greater than or equal to 1010 CFU / g, the saccharomycetes are selected from at least one of saccharomyces cerevisiae, phaffia rhodozyma, rhodotorula mucilaginosa and candida utilis, the acid-producing bacteria are selected from at least one of lactobacillus plantarum, enterococcus faecalis, enterococcus faecium, clostridium butyricum and lactobacillus rhamnosus, and the bacillus is selected from at least one of bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus amyloliquefaciens and bacillus megatherium. According to the composite microecological preparation and the application thereof, the intestinal health level of animals can be improved, the growth of the animals can be promoted, the anti-stress capability of the animals can be improved, the immune system of the animals can be activated, and diseases can be reduced.

The invention discloses rhodotorula mucilaginosa for removing total nitrogen in sewage and application of the rhodotorula mucilaginosa. The rhodotorula mucilaginosa NBUY-9 is is preserved in China Center for Type Culture Collection on December 5, 2019, the preservation number is CCTCC NO:M 20191015, the rhodotorula mucilaginosa grows on a YPD flat plate without seawater salinity, the bacterial colony morphology characteristic is orange red, the surface is round, smooth, wet and opaque, the strain can grow in a relatively wide pH range of 2.5 to 9.0, and the optimal growth temperature is 20-30DEG C. The rhodotorula mucilaginosa can be applied to preparation of a total nitrogen removal agent, an ammonia nitrogen removal agent and a COD removal agent in domestic sewage and preparation of anammonia gas degradation agent in industrial waste gas, and has the advantages that ammonia nitrogen, total nitrogen and COD in the sewage can be efficiently removed at the same time, and ammonia gas components in the industrial waste gas can be efficiently degraded.

Provided are a microbial agent capable of making a side slope and bulkhead vegetation community free of artificial maintenance and a construction method thereof. The microbial agent is prepared through the steps of preparation of fermentation raw materials, smashing of the fermentation raw materials, liquefaction, sterilization, fermentation, desiccation and packaging, and desiccation and packaging are conducted on the raw materials which are subjected to fermentation. The microbial agent comprises various fungi such as paenibacillus sp., bacillus amyloliquefaciens, arthrobacter humicola, pseudomonas graminis, phanerochaete chrysosporium, trichoderma longibrachiatum, aspergillus niger, rhodotorula mucilaginosa, sporobolomyces and clitocybe geotropa. According to the microbial agent free of artificial maintenance for the side slope and bulkhead vegetation community, by means of a nutrient substance circulation theory of dry branches and fallen leaves, the microbial agent is added to a target vegetation community in one time, in situ degradation is conducted on the dry branches and fallen leaves to activate microorganisms, in an ecological system, a new system which has the functions of matter circulation, energy flow, soil fertility increase and vegetation community update and succession is formed among the dry branches and fallen leaves, soil and vegetation, therefore, artificial cultivation of the side slope and bulkhead vegetation community is not needed, and plants grow vigorously.

The invention discloses a method for enhancing mussel astaxanthin fermentation production yield, which comprises the following steps: by using rhodotorula mucilaginosa as a fermentation strain, inoculating 2-10% of the rhodotorula mucilaginosa into a culture medium, and adding calcium carbonate and magnesium sulfate into the culture medium, wherein the addition amount of the calcium carbonate is 0.1-0.5 g / L, and the magnesium sulfate is magnesium sulfate heptahydrate with the addition amount of 0.1-0.7 g / L; and fermenting in a 5L fermentation tank for 3 days while regulating the ventilation quantity and rotation speed of the fermentation tank and keeping the fermentation temperature at 25-33 DEG C and the pH value at 5.5-7.5. On the premise of ensuring the product yield, the method can lower the energy consumption and reduce the fermentation operating cost by controlling the ventilation quantity and rotation speed in different fermentation stages, and thus, has important meanings for reducing industrialized production cost.

A Rhodotorula mucilaginosa strain ZZR-1# is named as Rhodotorula mucilaginosa, is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No.8890. The method using Rhodotorula mucilaginosa ZZR-1# to produce cutinase comprises the following steps: carrying out slant culturing on the Rhodotorula mucilaginosa by a slant culture medium, carrying out shaking seed culture, enlarged culture, enlarged culture and fermentation tank culture, filtering the obtained fermentation liquid by a gauze, and centrifuging the obtained filtrate at 4DEG C under a rotating speed of 5500rpm for 30min to obtain a supernatant which is a crude cutinase liquid; and concentrating the crude cutinase liquid, and purifying to obtain cutinase. The method using the Rhodotorula mucilaginosa ZZR-1# to produce cutinase is designed against the characteristics of the strain, and has the advantages of reasonability, simplicity, low cost, high enzyme activity in the crude cutinase liquid, high cutinase output, and good application prospect.

The invention discloses a method for improving biological prevention and control effect of rhodotorula mucilaginosa on fruit postharvest diseases. The method comprises the following steps of: activating the rhodotorula mucilaginosa, inoculating the activated rhodotorula mucilaginosa to an NYDB culture medium, culturing, and performing centrifugation to obtain bacteria; diluting the bacteria by using phytic acid solution to prepare 1*10<8> bacteria per milliliter bacteria suspension; putting fruits into the bacteria suspension, soaking the fruits for 30 seconds, immediately taking out the fruits, and naturally airing the fruits; and putting the fruits into a plastic basket, sealing the fruits by using a preservative film, and storing the fruits at room temperature or under a refrigerating condition. The method enhances the prevention and control effect of the rhodotorula mucilaginosa on the fruit postharvest diseases by using the phytic acid, and is simple in use, convenient for operation, good in effect and low in cost. The rhodotorula mucilaginosa with enhanced prevention and control effect can substitute a chemical bactericide to prevent and control the fruit postharvest diseases, so that harm of the chemical bactericide to the human body is avoided; and the method has remarkable economic benefit and social benefit.

The present invention refers to a process for obtaining copper nanoparticles from Rhodotorula mucilaginosa. The present invention refers to the use of dead biomass of Rhodotorula mucilaginosa to perform bioremediation of wastewater and for industrial scale production of copper nanoparticles.In the present invention, it is developed a synthetic strategy for the biosynthesis and removal of copper nanoparticles which is fast, low cost, environment friendly and easily scalable, using as a reduction agent the yeast Rhodotorula mucilaginosa.

The invention provides a rhodotorula mucilaginosa strain, a peanut cadmium-reducing agent and application thereof, which belong to the technical field of crop safety production. According to the peanut cadmium-reducing agent comprising a rhodotorula mucilaginosa fermentation liquor or a rhodotorula mucilaginosa solid preparation provided by the invention, the content of cadmium in peanut seeds canbe reduced. Meanwhile, the P content in a leaf, the yield of the peanut seeds and the biomass of a peanut root system can be also improved. The cadmium-reducing agent provided by the invention also comprises a sulfosalicylic acid solution with the mass concentration being 1 to 2g / L, so that the removal rate of the cadmium in the peanut seeds can be further improved. Compared with peanuts withoutbeing applied with the cadmium-reducing agent, according to the peanuts cultivated by adopting the cadmium-reducing agent provided by the invention, the cadmium content in the peanut seeds can be reduced by 40.63 percent to 44.78 percent, meanwhile, the phosphorus content is improved by 18.18 percent to 21.43 percent, the dry weight of the peanut root system is improved by 31.13 percent to 37.9 percent, and the yield of the peanut seeds is improved by 20.85 percent to 22.2 percent.

The invention discloses a probiotics microecological preparation and a preparation method and an application thereof. The probiotics microecological preparation comprises lactococcus lactis, bacillusamyloliquefaciens and rhodotorula mucilaginosa. The preparation method comprises the following steps: S1, preparing liquid preparations of strains; mixing the liquid preparations of the strains with bran in a weight ratio of 1: (4-11), airing the mixture at 40-60 DEG C, and crushing the mixture to obtain the solid preparations of the strains; and S2, preparation of the probiotics microecological preparation: uniformly mixing the liquid preparations of the strains to obtain probiotics microecological liquid preparations; and uniformly mixing the probiotics microecological liquid preparations toobtain the probiotics microecological preparation. The microecological preparation prepared by the invention is remarkable in synergic effect. The probiotics in the preparation are high in activity and rich in metabolite, the production performance of livestock in different stages can be improved, and the probiotics microecological preparation can be used as a feed additive and a substituent of antibiotics.

The invention discloses Tibet rhodotorula mucilaginosa YC1 and an application of the Tibet rhodotorula mucilaginosa YC1 to low-temperature storage of crystal pears, and belongs to the field of rhodotorula mucilaginosa. The preserving number of the Tibet rhodotorula mucilaginosa YC1 and the application is CGMCC No.10223. The Tibet rhodotorula mucilaginosa YC1 can be used for low-temperature disease control and low-temperature storage and freshness keeping of the crystal pears. An application method includes the specific steps that the rhodotorula mucilaginosa with the preserving number CGMCC No.10223 is activated and cultured in a fermentation mode, and obtained thalli are diluted through sterile water to prepare thallus suspension with the concentration of 1.0*10<7> CFU / ml to 1.0*10<9> CFU / ml; the crystal pears are soaked into the thallus suspension for 1 minute to 2 minutes, taken out, aired, placed into a sealed container and stored under the refrigeration condition of 3 DEG C to 5 DEG C, wherein the storage time can be 30 days.

The invention relates to the technical field of biological deodorization, and specifically discloses a compound microorganism deodorant and a preparation method thereof. The compound microorganism deodorant is microorganism fermentation liquor, and the method comprises the following steps: taking bacillus amyloliquefaciens CGMCC No.12917, Rhodotorula mucilaginosa CGMSS No.2.4008, enterococcus faecalis CGMCC No.1.2024 and acetobacter pasteurianus CGMCC No.1.2830 as the strain, performing pure breed expanding cultivation and mixed bacteria liquid fermentation culture, and finally obtaining the microorganism deodorant of viable bacteria at dormant state, wherein the total bacteria count of the microorganism strain is not less than 3.0*1010cfu / mL. The compound microorganism deodorant disclosed by the invention is good in deodorization effect and wide in application range, the provided mixed bacteria fermentation process is simple in preparation method, the production period is shortened, the utilization ratio of the production equipment is improved, the production cost is lowered, and the large-scale popularization and application is realized.