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Method for enhancing mussel astaxanthin fermentation production yield

A technology of red shrimp and green pigment, applied in the field of microorganisms, can solve problems such as high prices and failure to meet market demand, and achieve the effects of reducing production costs, reducing fermentation operating costs, and reducing energy consumption

Inactive Publication Date: 2015-08-12
WEIHAI LIDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many biotechnology companies in the United States, Canada, the European Union and other countries are working on the development and production of such products, but they are far from meeting market demand, so the price is relatively high

Method used

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  • Method for enhancing mussel astaxanthin fermentation production yield
  • Method for enhancing mussel astaxanthin fermentation production yield
  • Method for enhancing mussel astaxanthin fermentation production yield

Examples

Experimental program
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Effect test

Embodiment 1

[0027] This example provides a method for improving the yield of sea red astaxanthin produced by fermentation, which is realized by the following steps: using Rhodotorula gelatinum as the fermentation strain, according to the inoculum size of 2%, inoculating the rhodotorula gelatinous yeast into the culture medium , the medium includes: glucose 60g / L, yeast powder 3g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L, urea 1g / L and NaCl0.3g / L, the pH of this culture medium is 6.5, in described culture medium, add calcium carbonate and magnesium sulfate, adopt the fermentor of 5L to carry out fermentation, adjust in fermentation process The ventilation rate and the rotation speed of the fermenter, the fermentation temperature was maintained at 25°C, and the fermentation pH value was 5.5 for 3 days of fermentation.

[0028] The calcium carbonate is added in an amount of 0.1 g / L, and the magnesium sulfate is magnesium sulfate heptahydrate, and the added amount is 0.1-0.7 g / L.

[0029] T...

Embodiment 2

[0032] This example provides a method for improving the yield of sea red astaxanthin produced by fermentation, which is realized by the following steps: using Rhodotorula gelatin as the fermentation strain, and inoculating the rhodotorula gelatin into the culture medium according to the inoculum size of 10% In, the medium includes: glucose 130g / L, yeast powder 10g / L, KH 2 PO 4 1.5g / L, K 2 HPO 4 1.5g / L, urea 5g / L and NaCl9g / L, the pH of this culture medium is 7.5, adds calcium carbonate and magnesium sulfate in described culture medium, adopts the fermentor of 5L to carry out fermentation, regulates ventilation in fermentation process and the rotation speed of the fermenter, the fermentation temperature was maintained at 25°C, and the pH value was 7.5 for 3 days of fermentation.

[0033] The addition amount of described calcium carbonate is 0.5g / L, and described magnesium sulfate is magnesium sulfate heptahydrate, and addition amount is 0.7g / L.

[0034] The ventilation ra...

Embodiment 3

[0037] This example provides a method for improving the yield of sea red astaxanthin produced by fermentation, which is realized by the following steps: using Rhodotorula gelatinum as the fermentation strain, according to the inoculum size of 6%, inoculating the rhodotorula gelatinous yeast into the culture medium In, the medium includes: glucose 95g / L, yeast powder 7g / L, KH 2PO 4 1g / L, K 2 HPO 4 1g / L, urea 3g / L and NaCl4.65g / L, the pH of this culture medium is 6.5, add calcium carbonate and magnesium sulfate in described culture medium, adopt the fermentation tank of 5L to carry out fermentation, regulate ventilation during fermentation The fermentation temperature was maintained at 31°C, and the fermentation pH value was 6.5 for 3 days.

[0038] The addition of described calcium carbonate is 0.3g / L, and described magnesium sulfate is magnesium sulfate heptahydrate, and addition is 0.4g / L.

[0039] The ventilation rate is 1.65L / min in 1-12h during the fermentation proce...

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Abstract

The invention discloses a method for enhancing mussel astaxanthin fermentation production yield, which comprises the following steps: by using rhodotorula mucilaginosa as a fermentation strain, inoculating 2-10% of the rhodotorula mucilaginosa into a culture medium, and adding calcium carbonate and magnesium sulfate into the culture medium, wherein the addition amount of the calcium carbonate is 0.1-0.5 g / L, and the magnesium sulfate is magnesium sulfate heptahydrate with the addition amount of 0.1-0.7 g / L; and fermenting in a 5L fermentation tank for 3 days while regulating the ventilation quantity and rotation speed of the fermentation tank and keeping the fermentation temperature at 25-33 DEG C and the pH value at 5.5-7.5. On the premise of ensuring the product yield, the method can lower the energy consumption and reduce the fermentation operating cost by controlling the ventilation quantity and rotation speed in different fermentation stages, and thus, has important meanings for reducing industrialized production cost.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for increasing the yield of sea red astaxanthin produced by fermentation. Background technique [0002] Sea red astaxanthin is a kind of carotenoid with strong anti-oxidation ability and biological coloring ability. It has been widely used in food, medicine, cosmetics, feed and many other fields. It has high application value in preventing cardiovascular and cerebrovascular diseases, protecting eyes and central nervous system, preventing ultraviolet radiation, inhibiting tumors, anti-inflammation, and relieving exercise fatigue. Sea red astaxanthin, a carotenoid with high antioxidant capacity, its mobility is close to natural astaxanthin ester in thin layer chromatography, and its antioxidant capacity is also close to natural L-astaxanthin, so it is named, DPPH The method detects that its antioxidant capacity is about 40% stronger than that of natural L-astaxanth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/645
Inventor 魏升宁张文丽张美丽李纪丰孙常明程振伟
Owner WEIHAI LIDA BIOTECH
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