1180 results about "Mussel" patented technology

The invention relates to a method for separating and purifying mussel mucin by using a mixed adsorption chromatography. Mussel mucin contains a group of L-3,4- -Dihydroxyphenylalanine (L-DOPA), a phenohydroxyl group thereof can act as the supplier for hydrogen bond, the benzene ring thereof can generate a hydrophobic effect, and the lysine thereof with strong positive charges is capable of forming a static bond. On the basis of the properties of mussel mucin, a mixed adsorption chromatography (i.e. the adsorption chromatography based on three principles of adsorption with hydrogen bond, adsorption with hydrophobic effect, and static adsorption) is adopted to overcome the problem of low yielding rate of mussel mucin in the prior art for separating and purifying mussel mucin. An strong acid extraction is adopted to eliminate small-molecular compounds from a desalting column, an argar medium with high concentration and high cross-linking degree to separate and purify mussel mucin, and an acetic acid-urea- polyacrylamide gel electrophoresis is used to differentiate mussel mucin through specific chromogenesis with nitro blue tetrazolium. Three principles adopted with one separation medium to separate mussel mucin achieve high selectivity, simplify the purification technology, and decrease production cost.

The invention relates to a method for separating and purifying the sea-mussel mucin by using salting out and dialyzing, the salting out and the dialyzing utilizes the proteinaceous physicochemical character to purify by a non- chromatograph method, and solves the problem of high apparatus and material cost in the chromatograph method for purifying the sea-mussel mucin, a quick, low-cost and high-concentration method for separating and purifying the sea-mussel mucin is provided. The strong acid is used for extracting, the salting out and the dialyzing are used for purifying, the sea-mussel mucin is determined by adopting the acetic acid-carbamide- polyacrylamide gel electrophoresis via the blue tetrazolium specificity colour development, and the purity is determined by the inversed phase chromatography quantitatively.

The invention relates to a method for the eutrophication treatment of deepwater of reservoirs. The method is characterized in that an ecologic idea is used as a core guiding idea of treatment to build or restore a complex ecological water system structure in order to restore the self functions of the ecologic water system. Proper physical measures are combined on the basis of knowing the reservoir characteristics to realize the development toward a healthy direction. The method mainly comprises the steps of (1) control of the pollution source in a river basin; (2) reasonable arrangement of the flow direction of water; (3) plantation of aquatic higher plants or herbaceous plants on a floating bed; (4) plantation of submerged plants on a platform in water; (5) change of the fish structure; (6) stocking and hanging breeding of benthonic animals comprising mussels and snails; (7) hanging of a biological membrane in order to provide an adherence for microorganisms and the like; (8) construction of a constructed wetland or plantation of emerged plants in the shallow position of the tail of water reservoirs; and (9) plantation of drought and flooding tolerant herbaceous plants in the falling zone. The method does not bring secondary pollution to the water in the treatment process, and is especially suitable for the eutrophication treatment of water in a water source.

The invention relates to an ecological trepang culture method in silt sandy ponds in coastlands, which belongs to the aquaculture field. The method comprises the steps of setting a pond, setting trepang reefs, disinfect the pond, controlling feed water, cultivating feedstuff, stocking young trepang and managing culture. The feedstuff culture which is performed in April to May comprises the following steps: firstly, transplanting benthic diatom in a height of 0.8 to 1 m feed water depth in the pond in the prior period, with 5 to 10 L of benthic diatom liquid per Chinese acre; secondly, transplanting ruppia maritime and amphilhoe japonice, especially, integrally transplanting ruppia maritime through pulling up by the roots according to the quantity of 5 to 15m per square meter, and transplanting amphilhoe japonica according to the quantity of 1.5 to 3 kg per Chinese acre after the transplanting of ruppia maritime; thirdly, poly-culturing mussel organism; fourthly, feeding water to the pond to the depth of 3 to 4 m, and stocking prawns according to the quantity of 500 to 1500 per Chinese acre. In the method of the invention, ecological feedstuff is adopted for transplanting and polyculture, therefore, not only the cost of artificial feedstuff is saved, but also the natural ecological environment is created for trepang culture.

The invention relates to a mussel meat protein antioxidative peptide and a preparation method thereof. The invention is characterized in that the antioxidative peptide is a pentapeptide compound with an amino acid sequence of YPPAK (Tyr-Pro-Pro-Ala-Lys), and according to ESI-MS detection, a molecular ion peak of m / z [M+H]<+> 575.26 is given out. The preparation method comprises the steps consisting of slurry homogenizing, degreasing, mixing, enzymatic hydrolysis, desalination, ultrafiltration, chromatography and the like. The prepared high-activity antioxidative peptide YPPAK (Tyr-Pro-Pro-Ala-Lys) has a good removal effect on DPPH free radicals, hydroxyl free radicals and superoxide anion free radicals; meanwhile, the YPPAK (Tyr-Pro-Pro-Ala-Lys) shows a good inhibitory effect on lipid peroxidation. The YPPAK (Tyr-Pro-Pro-Ala-Lys) has the advantages of safety, no toxic and side effects, good antioxidant activity, easy digestion and absorption, etc., and can be used as a medicine, a health food or foodstuff additive and the like.

Disclosed is an artificial micro-particle feedstuff for pseudosciaenacrocea larvae, which comprises white fish meal, shrimp powder, mussel powder, calamari powder, yeast powder, hydrolyzed fish meat protein, fish oil, alpha-starch, sodium alginate, mineral salts mixture, vitamins mixture, feeding promoting agent, tomatine, ethoxy quinoline, beta-gluglucosan and soya bean lecithin by the weight ratio of 35-45, 10-20, 10-15, 8-12, 3-6, 5-10, 3-6, 6-12, 1-3, 1-3, 1-3, 0.1-0.2, 0.01-0.05, 0.05-0.08, 0.1-0.3 and 5-8.

The invention relates to microbial fermentation bait, in particular to a method for producing microbial fermentation bait for sea cucumbers. Soya bean meal, bran, rice bran, soy sauce residue, mussels, algae and sea floating sludge taken as fermentation raw materials, and Saccharomyces cerevisiae, Candida, Bacillus subtillis, lactobacillus and photosynthetic bacteria taken as beneficial microflora are subjected to inoculation at a temperature of between 25 and 30 DEG C for solid fermentation to produce the microbial fermentation bait for sea cucumbers. The method has the characteristics of simple production process, easy operation, wide sources of production raw materials, low cost, easy promotion, short production period, more fermentation products, good product quality, easy application and the like. When applied in sea cucumber culture, the microbial fermentation bait can improve the survival rate and immunity of the sea cucumbers, quicken the growth and development of the sea cucumbers, improve the ecological environment of culture pond water and purify water quality, and is ideal good-quality microbial fermentation bait for the sea cucumbers.

The invention discloses a biogas slurry ecological purification device, which comprises an adjusting tank (1), a facultative tank (2), an aerobic tank (3), a fish culturing tank (4) and a clarifying tank (5), wherein all tanks have a liquid outlet pipe and a liquid inlet pipe; the liquid outlet pipe of a tank is communicated with the liquid inlet pipe of a next tank and a control valve (8) is arranged between the liquid outlet pipe of the tank and the liquid inlet pipe of the next tank; effluent of the clarifying tank (5) passes through a three-way pipe (6) and a control valve of the three-way pipe (6) to be connected with a discharge pipe and a return pipe (7) communicated with the adjusting tank (1); the liquid outlet pipe of the adjusting tank is provided with a flow meter (14); the return pipe (7) is provided with a circulating water pump (11); and the aerobic tank (3) is provided with an aeration device (12) and a filler. Biogas slurry purification by the device is realized by culturing water hyacinth in the facultative tank (2), algal-bacteria, plankton and mussel in the aerobic tank (3) and fish in the fish culturing tank (4), using the algal-bacteria and the plankton as fish baits, and clarifying the effluent till the effluent meets national standards for integrated wastewater discharge. The device has the advantages of high speed, high efficiency, circular purification and diversified operations.

The invention relates to a seasoning material product prepared by applying cooked juice of mussels and belongs to the technical field of processing and utilization of side products of shellfishes, and aims at providing a seasoning material prepared by utilizing concentrated liquid of cooked juice of the mussels and a preparation method of the seasoning material. The seasoning material product is prepared by the following specific steps: firstly properly diluting the concentrated liquid of the cooked juice of the mussels, and adjusting the pH to 6.0-7.0; adding papain with the weight being 0.5-0.8% of that of the cooked juice, stirring uniformly, and hydrolyzing for 2h-5h at the temperature of 40-60 DEG C; then carrying out enzyme deactivation by water bath; carrying out centrifugation and deslagging on enzyme deactivating liquid, adding 2.5-4.5% of yeast and 2.0-4.0% of active carbon to carry out debitterization and deodorization treatment in sequence, and filtering and centrifuging to obtain deodorized liquid; adding 1.0-3.0% of reducing sugar, 1.0-3.5% of amino acid and 0.2-0.8% of a flavoring agent according to the weight of the deodorized liquid, heating for 30-70min on a heating device with the temperature of 90-130 DEG C and then carrying out Maillard reaction; and finally sterilizing a reaction product to prepare the product. The seasoning material product and the preparation method have the advantages that a method for combining a bio-enzyme control enzymolysis technology and the Maillard reaction is adopted for preparing the seasoning material of seafood. By measurement, the content of the amino acid in the product is rich, and the product has certain health action; in the aspect of flavor, not only is the original seafood flavor of the mussels maintained, but also the characteristic that flavor is formed by the Maillard reaction is utilized to enable the product to enhance the flavor and cover the odor.

The present invention relates to an aquatic organism sampling device, and specifically is a sampling shovel for easy acquisition of freshwater mussels attached in water pipes and benthic organisms in shallow water sediments. The sampling shovel is composed by a sampling container, an open and close device for a sampling container cover, and an angle adjustment device. By a self-closing device and a pulling open device, the open and the close of the sampling container can be controlled, and the angle between a holding rod and the sampling container can be adjusted. The sampling shovel of the invention has the advantages of simple structure, convenient operation, high controllability, reliable and flexible operation, and low cost. The sampling shovel can be applied for the operation processes of acquiring the freshwater mussels attached in the water pipes and such unavailable samples at high places, and sampling the benthic organisms in the shallow water sediments.

The invention relates to a culture raft frame for scallop and mussel culture, which is suitable for artificial culture of marine products including shallow sea scallops and mussels. The culture raft frame comprises a large culture stalk, inflation and exhaust type buoys, a culture cage, pulleys, an inflation and exhaust hose and an air valve core sealing valve, wherein the large culture stalk is connected with the buoys by regulating cables, one buoy is corresponding to a regulating cable, a universal lock catch is arranged below each buoy, a water depth graduation mark is arranged on each regulating cable, the inflation and exhaust type buoys are uniformly arranged on the large culture stalk and connected with the large culture stalk by cables and cable padlocks, water charging and drainage holes are uniformly arranged below the inflation and exhaust type buoys along the circumferential direction, and double pulleys are respectively arranged at both ends of the large culture stalk. The raft frame can realize upward floating and sinking of the large culture stalk within a depth range of 2-12m under the sea level, thereby satisfying various requirements of the cultured marine products on water depth and water temperature in different sea conditions and different seasons.

A support apparatus adapted to support mussels in an aquatic mussel harvesting operation. The apparatus has a support extension that is adapted to be inserted into a harvesting support that is adapted to facilitate growth of mussels. An upper surface is provided to give the aquatic mussels support.

The invention discloses a mytilus edulis enzymolysis polypeptide. The mytilus edulis enzymolysis polypeptide is characterized by containing the following amino acid sequence: Asp Leu Tyr. The mytilus edulis enzymolysis polypeptide is prepared by adopting the following steps of: (1) preparing homogenate from mytilus edulis meat, adding alkaline protease, deactivating the protease, centrifuging, and taking clear solution of the upper layer; (2) performing ultra-filtration on the clear solution, collecting hydrolysate with the molecular weight of below 3K, concentrating, and performing freeze drying; (3) performing chromatographic separation by adopting a DEAE-SepharoseFF ion exchange column; (4) performing chromatographic separation by adopting a Sephadex G-25 gel column; and (5) performing high performance liquid chromatography purification. The invention also discloses application of the mytilus edulis enzymolysis polypeptide prepared by the steps in prostatic cancer resistance. Compared with the prior art, the invention has the advantages that: the mytilus edulis is subjected to enzymolysis and purification by adopting an optimal protease and an optimal technology, a strong cell proliferation inhibiting effect is achieved when the obtained target peptide is applied to prostatic cancer resistant cells, and a feasible research path is provided for resisting prostatic cancer.

The invention discloses a mussel shell removing machine which belongs to shell removing machines. The mussel shell removing machine comprises an opening extension box, a shell removing box and an oscillating screen, wherein the lower part of the opening extension box is provided with a transmission belt; the lower part of the transmission belt is provided with the shell removing box; the lower part of the shell removing box is provided with the oscillating screen; the top of the opening extension box is provided with a feed opening, and the inner part is provided with a flexible impeller; theflexible impeller is connected with a first motor; four symmetrical brushes are arranged in the shell removing box; convex groove plates are respectively arranged between the two brushes at the upperpart and both sides of the two brushes at the lower part; uneven objects are arranged on the convex groove plates; the oscillating screen is connected with a screen frame by a cranked lever; the upperpart of the oscillating screen is connected with the screen frame by a support tension spring; a plurality of screen holes are arranged on the oscillating screen; and the brushes, the oscillating screen and the transmission belt are respectively connected with a second motor. The mussel shell removing machine has the characteristics of realizing automated production, solving the problems of mussel extrusion, and the like due to labor force shortage in the producing process of mussels, and the like.

The invention relates to an eel feed. The eel feed consists of the following components in percentage by weight: 30 to 55 percent of mussel powder, 8 to 15 percent of corn protein powder, 2 to 4 percent of milk powder, 7 to 10 percent of chicken blood powder, 1 to 4 percent of duck blood powder, 4 to 7 percent of sorghum protein powder, 10 to 20 percent of cabbage worm powder, 1 to 2 percent of mineral additive, 2 to 5 percent of vinasse, 3 to 7 percent of cod liver oil and 1 to 2 percent of vitamin blending agent. The method for preparing the eel feed comprises the following steps of: pouring the components into a blender in a certain ratio to mix and stir uniformly; adding quantitative grease and water into the mixture; stirring the mixture into a sticky dough shape; and feeding the eel after sieving the mixture by using a 60-mesh sieve.

The invention belongs to the technical field of cultivation, and relates to a method for cultivating large granular nuclear pearls. In order to solve the problem that in a traditional process for cultivating nuclear pearls, after pearl cores are inserted, pearl cultivation creature (e.g. freshwater mussel and the like) generally has high pearl production rate, the invention provides a method for cultivating large granular nuclear pearls. The method comprises the following steps that: a. outer mantle epithelial cells are obtained from mussel body; b. the pearl cores are incubated from the obtained outer mantle epithelial cells, and incubated pearl cores are obtained; c. the incubated pearl cores are implanted into pearl cultivating mussel to be cultivated, wherein, step c is divided into the following sub-steps that: (1) a sac for containing the incubated pearl cores is arranged in the pearl cultivating mussel; (2) the incubated pearl cores are stuffed into the sac, and the opening of the sac is sutured by a biological suture; and (3) the pearl cultivating mussel treated in step (2) is cultivated. The method of the invention can smoothly solve the technical problem, so that the diameter of the cultivated pearls is generally above 10mm.

The invention provides a marine ranch platform, and belongs to the technical field of aquaculture equipment. The marine ranch platform comprises four upright columns and mussel floating bodies. The four upright columns are vertically arranged, connection lines of the centers of the four upright columns are rectangular, a second guide rod is vertically arranged on each upright column, four third guide sleeves are arranged on each rectangular mussel floating body, the mussel floating bodies are arranged on the outer sides of the four upright columns, the third guide sleeves are in one-to-one correspondence to the second guide rods, third guide holes are formed in the third guide sleeves, the third guide sleeves are slidably arranged on the corresponding second guide rods via the third guide holes, a plurality of eye plates are arranged on the lower side surface of each edge of each mussel floating body, a mussel aquaculture rope is connected onto each eye plate by a shackle, and balancing weights are arranged at the lower ends of the mussel aquaculture ropes. The marine ranch platform has the advantages that the marine ranch platform can bring convenience for farming mussels in a free-range manner and harvesting the mussels, the mussels can be prevented from being accumulated at falling tide, mixed aquaculture can be carried out on the mussels and fish, and the like.

The invention discloses a jewfish fish meal-free high efficiency safety mixed feed, which comprises the following components: 5-10 parts of chicken powder, 2-6 parts of pork powder, 5-15 parts of soy protein concentrate, 2-8 parts of fermented soybean meal, 2-7 parts of corn gluten meal, 12-25 parts of krill meal, 5-10 parts of cuttlefish powder, 1-5 parts of silkworm chrysalis powder, 3-6 parts of mussel housefly larvae enzymatic hydtolysis peptide powder , 3-5 parts of probiotics fermentation small peptide mixture, 1-3 parts of molasses extract yeast, 1-3 parts of seaweed meal, 12-18 parts of high gluten wheat flour, 1-4 parts of refine fish oil, 1-3 parts of refine soya-bean oil, 2-5 parts of soybean phosphatide oil, 0.1-0.4 parts of choline, 0.2-0.5 parts of composite vitamin, 1-3 parts of composite mineral matter, 1-3 parts of calcium dihydrogen phosphate, 1-2 parts of calcium lactate, 2 parts of traditional Chinese medicine compound preparation, 2 parts of premix and 2 parts of additive. The feed has the characteristics of no fish meal, fast growth, low bait coefficient, good stability in water, fast ingestion, low feeding cost, high efficiency and safety.

The invention relates to an underwater revetment based on ecological bags and a water body ecological management method. The method comprises a first step of dredging and solidifying, a second step of establishing a water bottom foundation, a third step of clearing up the revetment surface, a fourth step of paving the ecological bags in a step-shaped lamination dislocation mode from the top of the water bottom foundation, a fifth step of planting submerged plants on the ecological bags, a sixth step of stopping river closure or beginning to coffer after the water quality conforms to or superior to the fourth scale of the national surface water environment criterion GB / 3838-2002, wherein when the water quality does not conform to or inferior to the fifth scale of the GB / 3838-2002, microbial preparation is added into the water according to the water quality measuring result, and a seventh step adding filter feeder fishes or / and herbivorous fishes, shrimps and demersal mussel animals. The method guarantees that floodwater can be drawn off based on the fact that the underwater revetment structure of rivers and lakes is safe, and the cooperative management capacity for the water body is improved through ecological restoration of the underwater revetment.

The invention relates to a small water turtle breeding feed and a preparation method thereof. The small water turtle breeding feed is prepared from the following raw materials in parts by weight: earthworm powder, Chinese yam powder, extruded soybeans, beer yeast, cod-liver oil, pork tripe meal, mussel meal, amino acid, monocalcium phosphate, mineral substances and traditional Chinese medicine powder, wherein the traditional Chinese medicine powder comprises bighead atractylodes rhizome, medicated leaven, malt, rice sprout, embelia laeta and ajuga nipponensis. Compared with the prior art, the small water turtle breeding feed is prepared according to the growth requirements of small water turtles, is rich in nutrition and free of hormone and chemical residue and is capable of promoting the appetite and digestion of the small water turtles.

The invention discloses a preparation method of multifunctional group bionic mussel adhesive protein polymer. The preparation method comprises four processes: the synthesis and preparation of polysuccinimide prepolymer powder, the synthesis and preparation of water soluble polysuccinimide derivative, the synthesis and preparation of polyaspartic acid derivative, and the synthesis and preparation of bionic mussel adhesive protein polymer. The preparation method utilizes the characteristics of environment protection and nontoxicity of polysuccinimide and the characteristics of adhesive protein and organic coating, sufficient catechol groups introduced to a structure can perform complexation with metal ion on the surface of metal base materials to form a passive film, so that good adhesion is formed on the surface of the metal base materials, active epoxy groups and hydroxyl easily cured and crossly bonded with the organic coating are introduced into the molecular structure, the interface chemical bonding probability between the polymer and the organic coating is improved, the adhesive strength between the polymer and the organic coating is realized, and the protective performance of the whole protective coating is improved.

The invention relates to a treatment method of middle-late stage attaching bases in shellfish seedling breeding, in particular to a method for treating the attaching bases (mesh sheets, red palm ropesand the like) of shellfish (scallops, mussels, arca inflata reeves and the like) by using the benthic diatom film method, thereby greatly improving the larval attaching rate and the metamorphosis rate. The method has convenient operation and can treat a large number of attaching bases. The treatment method and the attaching bases after treatment have no pollution and are safe and environment-friendly; more importantly, the seedling attaching effect is significantly better than the attaching bases treated by using the traditional method.

The present invention relates to a bioadhesive derived from mussel. In particular, it relates to a novel Mytilus galloprovincialis foot protein type 5 (MGFP-5) and a recombinant protein that is a hybrid of MGFP-5 and foot protein type 1 (FP-1), where an adhesive protein with adhesive activity can be economically mass-produced to be used in place of chemical adhesives through the present invention.

The invention discloses a pearl cultivation method which relates to cultivation, adopting the steps as follows: A. a pearl nucleus is inserted into the abdominal foot of a freshwater mussel; B. the freshwater mussel inserted with a pearl nucleus is put back into an aquaculture water area for culture; and C. a pearl is obtained after the pearl nucleus cultured in the freshwater mussel is matured; wherein, the step A comprises the steps: A1. cell scraps are attached on the pearl nucleus; A2. the freshwater mussel mouth is opened and a nick is cut inside the abdominal foot of the freshwater mussel by using a recessing tool; and A3. the pearl nucleus and cell scraps are inserted into the nick simultaneously; wherein, the step A1 comprises the steps: A11. a through hole is opened on the pearl nucleus; A12. an inserted link provided with a pinhead is adopted and the pinhead passes through the through hole; and A13. the end of the pinhead passes through the cell scraps and leads the cell scraps to be attached on the surface of the pearl nucleus; the step A3 comprises the steps: A31. the inserted link sends the pearl nucleus and cell scraps on the pinhead into the inner end of the nick; and A32. the inserted link is pulled back to cause the pearl nucleus and cell scraps to be separated from the pinhead and left in the nick. The method has low cost and is characterized by high value, thus having higher cost performance.

The invention relates to a method for realizing ecological larval culture on rapana venosa. The method is characterized by comprising the following steps of: firstly selecting a pond which is convenient in water exchange, takes lithoherm and stone bottom as a substrate and is in the size of 2-5Mu; then placing mature rapana venosa with the length of 7-10cm into the pond in the quantity of 100kg per Mu, mating and spawning after the rapana venosa eats ruditapes and mussel for 2-3 days, when enough oocysts are obtained, removing the rapana venosa away, incubating the oocysts for 20-22 days at the water temperature of 20-25 DEG C to obtain planktonic larva, growing the planktonic larva by eating the planktonic larva in the pond, starting to throwing polyethylene corrugated plate adherence ortile under the condition that the water temperature is 22-25 DEG C after 15-16 days, sticking benthic diatom and the planktonic larva onto the polyethylene corrugated plate or tile, transfigurating the planktonic larva of the rapana venosa into young spiral shell after 5-6 days, growing the transfigurated young spiral shell by eating the planktonic larva and benthic diatom on the corrugated plate, wherein the survival rate of the transfigurated young spiral shell on the corrugated plate can reach more than 50%, and feeding small low-value bivalve to the young spiral shell with the length morethan or equal to 3mm. By applying the method provided by the invention, the defects that the survival rate is low and the larval yield is low as bait conversion is difficult and water is polluted when carnivorous fish mice are used as feed in indoor artificial larval culture can be overcome, and the larval yield is improved by more than 50% compared with the indoor artificial larval culture. Meanwhile, the production cost is saved as no bait is thrown into the pond, thus the method is environment-friendly, and the produced larval is strong.

The invention provides a method for breeding juvenile mussels of hyriopsis schlegeli. The method is characterized by comprising the following steps of cultivation of breeding mussels, larva collection, cultivation of juvenile mussels at a parasitic stage, cultivation of juvenile mussels after juvenile mussel escape and cultivation of small mussels. Preferably, the breeding mussels are 4 to 5 yearsold mussels; the breeding mussels are selected in late autumn, and the selected breeding mussels are cultivated in a special pond; the host fish is pelteobagrus fulvidraco; the host fish is fed withthe mussel meat for nutritional fortification cultivation; the density of glochidia attached to the host fish is 30,000 to 40,000 per kilogram of the host fish; and in the steps of the cultivation ofjuvenile mussels at the parasitic stage, the cultivation of juvenile mussels and cultivation of small mussels, the stocking densities are 1 to 1.5kg / m<2>, 20,000 to 30,000 / m<2> and 150 to 250 / m<2>respectively, and the flow rates are controlled to be 1 to 1.5T / h, 0.5 to 2T / h and 2 to 3T / h respectively. Each day, the nutritional mud is quantitatively added once, and the pond is at least cleanedtwice. By the method, the rate of emergence of the juvenile mussels of the hyriopsis schlegeli is 30,000 to 40,000 mussels / m<2> and improved by 30 percent; and the juvenile mussels bred by the methodhave strong physique and order specifications.

The invention discloses a polymer represented by a formula I as well as a preparation method and application of the polymer. In the formula I, the molecular weight is 50000-120000; the ratio of x to y is 1:(0.25-0.75); n is 10-50; n1=2 or 3; R1 is any one selected from the group of C1-C4 alkyl and phenyl; and R2 is anyone selected from the group of groups represented by a formula II and a formula III. The preparation method comprises the step of reacting N-(3-amino alkyl) methacrylamide hydrochloride containing amino, dyhydroxyl substituted ortho aromatic aldehyde, phosphite ester and methylacrylic acid polyethylene glycol single methyl ether in an organic solvent in the presence of triethylamine, an initiator and a chain transfer agent. The macromolecular adhesive prepared by the invention is a water-based adhesive, which is mussel-mimetic and environmentally friendly; the macromolecular adhesive is applied to adhering among a plurality of substrates; adhesion can be realized under a variety of conditions; the macromolecular adhesive has good adhesive power; furthermore, the macromolecular adhesive is simple in preparation method, abundant in raw material source and low in cost; and therefore, the macromolecular adhesive is a potential excellent adhesive material.

The invention relates to a method for preparing a pollution-proof surface material suitably applied in the marine environment. The method comprises that: vinyl end capped dimethyl silicone polymer, hydrogen contained silicone oil, a platinum catalyst and polyacrylamide microcapsules are evenly mixed and stirred and sulfated at normal temperature to prepare the pollution-proof surface material of which hydrophilic surfaces and hydrophobic surfaces are distributed at intervals; the hydrophilic polyacrylamide microcapsules are evenly distributed on the material surfaces, and after the surface material is soaked in water, a local hydrophilic area with even distribution is formed, and the local hydrophilic area and the hydrophobic organic silicon surfaces are regularly distributed at intervals; polluted marine lives are not easy or not tight to be attached to the hydrophilic-hydrophobic surface, thereby preventing pollution; the pollution-proof surface material can strongly inhibit the attachment of marine lives such as diatom, mussels, and the like, and the attached quantity of mussel byssus is reduced by more than 50 percent; moreover, the surface material is environment friendly, nontoxic and unharmful.

The invention discloses a reduced graphene oxide emulsion separating film, and a preparation method and application thereof. The preparation method comprises the following steps: 1) mixing graphene oxide, a reducing agent and a buffer solution and carrying out a reaction so as to obtain a reacted solution; and 2) subjecting the reacted solution to vacuum pumping filtration and drying so as to obtain the reduced graphene oxide emulsion separating film. The film can be used for separation of a variety of nanometer / submicron oil-in-water type emulsions, has the advantages of high separation efficiency, reusability, self-supporting performance, etc. and the special infiltration property of super-hydrophilicity / underwater super-oleophobicity, is capable of resisting strong acid, weak base and high salt environments, shows good stability and can be cyclically used many times. The film is synthesized by using a mussel bionic process; raw materials are mild, nontoxic and easily available; preparation process is simple, labor-saving and time-saving; preparation of the emulsion separating film on a plurality of filter membrane substrates is realized; and large-scale production and application can be implemented.

The invention discloses an anti-bacterial agent and a surface remover which backed with a plurality of mixed shells. The preparation method of the invention is that at least two kinds of shells are chosen to be mixed from oysters, clams, small abalones, thick shell mussels, north pole shells and scallops, the mixed shells are heated in an electric furnace to 600 DEG C to 1200 DEG C at last, the mixed shells simultaneously undergo the burning and crushing processes to become the anti-bacterial agent and the surface remover of which sizes of particles are between 0.1 um to 150 um and sizes of average particles are between 2 um to 60 um. The anti-bacterial agent and the surface remover made according to the technical proposal of the invention has a better bacteriostastic effect compared with prior art, removing pesticides and polishes left on the agricultural product surfaces and applying to such technical field as human body microelements supply and water treatment etc..