A mandarin fish feed rich in zinc and organic selenium and a preparation method thereof are disclosed. The preparation method comprises the following steps: crushing all raw materials including soybean meal, peanut powder, rapeseed meal, fish meal, soybean meal, river snail powder, shell powder, cuttlefish paste, cuttlefish viscera powder, oyster powder, radish powder, selenium-rich corn flour, selenium-rich malt meal, selenium-rich california burclover, bone meal, duck intestine powder, vitamin C, an immunopotentiator and fish auxin and mixing, hardening and tempering the mixed semi-finished product through a conditioner by introducing water vapor for 3-15 min, and granulating by a pressure granulator at the granulation temperature of 90-95 DEG C so as to obtain feed particles. The feed can remarkably raise survival rate and yield of mandarin fish. By eating the mandarin fish fed with the feed, human body capability and anticancer ability also can be raised, human immunity is enhanced, growth and development of teenagers are promoted, and cardiovascular and cerebrovascular diseases are prevented and treated. As cheap raw materials such as soybean meal, peanut powder, rapeseed meal, etc. are used as main raw materials, price is low. Thus, cost of the product is reduced.