30 results about "Palaemon carinicauda" patented technology

The invention relates to an ecological healthy culture method of Palaemon carinicauda. The ecological healthy culture method of Palaemon carinicauda includes firstly, selecting a pond; secondly, pretreating the pond; thirdly, feeding water; fourthly, medially inspecting and putting in parent shrimps; fifthly, controlling water quality; sixthly, performing polyculture of fishes; seventhly, putting in feed; and eighthly, performing culture management. The ecological healthy culture method of Palaemon carinicauda is simple in process and low cost. Water pollution caused by excess feed residue or excrement in the culture process of Palaemon carinicauda is reduced. Medicine residue in aquatics and waters due to massively using fishery medicines to prevent and control fish diseases is reduced. The ecological healthy culture method of Palaemon carinicauda is widely applicable.

The invention relates to a method for mixed culturing of swimming crabs and palaemon carinicauda. The method belongs to the technical field of fishery industry and is characterized by comprising the following steps of (1) clearing up a pond; (3) putting swimming crab seedlings in mid-to-end of May, selecting III-period baby crabs cultivated in a soil pool, putting 1-1.5kg per mu, in the end of June, putting 1-2kg of lively-swimming egg-carried shrimps per mu, and planting submerged plant water caltrop in the bottom part of the pond for improving water quality; (4) replacing 70-80 percent of water at every 5-7 days, sterilizing in time after water replacement, and putting water quality modifying agent for one time at the second day; (5) feeding crab and shrimp mixed feed, and then feeding according to the conventional mixed culturing method of the swimming crabs and palaemon carinicauda. According to the method for the mixed culturing of the swimming crabs and the palaemon carinicauda, the nutrition is balanced, the daily demands of the swimming crabs and palaemon carinicauda can be met, the feed efficiency is high, the survival rate of the swimming crabs and palaemon carinicauda is high, the ponderal growth is quick, the immunity of crab bodies can be remarkably enhanced, and the capabilities of antibiosis and antiviral of the crab bodies are improved.

The invention provides a method for completely and artificially breeding palaemon carinicauda parent shrimps indoors and belongs to the technical field of palaemon carinicauda artificial breeding. The method comprises the steps of water quality control, larval shrimp selection, density setting, water temperature control, feeding and water change, gonad development adjustment and gonad mature judgment. The water quality control is that water temperature is controlled to be 24-28 DEG C in the whole breeding process. The feeding and water change is that a matched feed is fed before the palaemon carinicauda grows to 2cm, clam meal and fresh alive clam worms are fed when the palaemon carinicauda grows to 2cm, and the daily water changing amount is 20%-30%. The gonad development adjustment is that when female shrimp ovaries develop to an II-III period, female shrimps identical in gonad development degree are bred in the same aquarium, and breeding water temperature of a group of shrimps with low gonad development speed is improved by 2-4DEG C. The gonad synchronous development and mature of palaemon carinicauda are achieved through feed selection, density setting and water temperature control in the breeding process of the palaemon carinicauda parent shrimps, and large-scale indoor artificial breeding of the palaemon carinicauda is facilitateed.

The invention belongs to the field of aquiculture, and in particular relates to an ecological cultivation method of the arca subcrenata lischke. The method comprises the steps of: treating the arca subcrenata lischke, stocking the arca subcrenata lischke, cultivating the arca subcrenata lischke in a covering way, adjusting the water quality, measuring in a sampling way, and controlling the disease. The arca subcrenata lischke is stocked according to six specifications such as the 4.2mm, 5.2mm, 6.4mm, 8.2mm, 11.6mm and 21.4mm, the stocking density is 450-550 granules / m<2>, and the growing condition of the six groups of the arca subcrenata lischke is compared with the surviving condition of the six groups of the arca subcrenata lischke. The method has the benefic effects that the stocking specification is reasonable by adopting the ecological cultivation method, the water quality is purified, the sewage discharge is reduced, the ecological environment is protected, the quality of the aquatic product is guaranteed by the three-dimensional ecological cultivation of the arca subcrenata lischke with the penaeus japonicus bate, the palaemon carinicauda and the barracuda, and the economic benefit is obviously improved.

The invention provides a method for manually hatching palaemon carinicauda indoors and preferentially selecting larvas. The method includes: A, preparing a hatching barrel; B, preparing water; C, selecting ovigerous parent shrimps; D, culturing parent shrimps during hatching period; and E, preferentially selecting the larvas. Every ovigerous shrimp is cultured separately indoors, so that after the lavas is hatched, genetic relationship is clear, and the method can be used for establishing families and selecting breeding. In addition, development rate and hatching time of a fertilized ovum can be adjusted by appropriately adjusting water temperature. The hatching barrel is white and semitransparent, the barrel bottom contacts with a black base and is light-proof, so that larvas with strong vigour can be collected at spotlight positions on the barrel wall.

The invention discloses a microsatellite marker primer and method for authenticating an inbred family of palaemon carinicauda, and belongs to the field of molecular biology. Microsatellite marker names are ES034 and ES096. The invention further provides a method for authenticating the inbred family of the palaemon carinicauda by using the primer. According to the microsatellite marker primer and the method, the genetic relationship among individuals in a palaemon carinicauda group is authenticated by using two pairs of the microsatellite primers to judge the inbreeding level among the individuals in the group; when all the individuals have monomorphism segments at 146bp and 263bp, the authenticated group meets the requirement of inbred cultivation. The method supplies a basis for judgment of an inbred line.

The invention provides a north small shed breeding method of sepia pharaonis. The method includes the steps of earth pond selecting and transforming, delivering of opossum shrimp and palaemon carinicauda berried shrimps, feeding of bait for opossum shrimp and palaemon carinicauda polyculture, delivering of sepia pharaonis fertilized eggs, breeding of young sepia pharaonis and feeding of bait for sepia pharaonis and shrimp earth pond polyculture and sepia pharaonis and shrimp polyculture. According to the method, opossum shrimp, palaemon carinicauda, the sepia pharaonis and the like are introduced into the same breeding pond, by means of pond transforming, small shed building, communicating pond breeding and developing of the bait for polyculture, the stability of an ecological system is improved, the occurrence rate of palaemon carinicauda diseases is effectively controlled, meanwhile, a water body is fully utilized, the productivity of a water area is improved, economic benefits are increased, the method is suitable for being popularized to outdoor shrimp ponds in the north of China, and north sepia pharaonis breeding industrialization is promoted.

The invention provides a method for completely and artificially breeding palaemon carinicauda parent shrimps indoors and belongs to the technical field of palaemon carinicauda artificial breeding. The method comprises the steps of water quality control, larval shrimp selection, density setting, water temperature control, feeding and water change, gonad development adjustment and gonad mature judgment. The water quality control is that water temperature is controlled to be 24-28 DEG C in the whole breeding process. The feeding and water change is that a matched feed is fed before the palaemon carinicauda grows to 2cm, clam meal and fresh alive clam worms are fed when the palaemon carinicauda grows to 2cm, and the daily water changing amount is 20%-30%. The gonad development adjustment is that when female shrimp ovaries develop to an II-III period, female shrimps identical in gonad development degree are bred in the same aquarium, and breeding water temperature of a group of shrimps with low gonad development speed is improved by 2-4DEG C. The gonad synchronous development and mature of palaemon carinicauda are achieved through feed selection, density setting and water temperature control in the breeding process of the palaemon carinicauda parent shrimps, and large-scale indoor artificial breeding of the palaemon carinicauda is facilitateed.

The invention relates to an ecological healthy culture method of Palaemon carinicauda. The ecological healthy culture method of Palaemon carinicauda includes firstly, selecting a pond; secondly, pretreating the pond; thirdly, feeding water; fourthly, medially inspecting and putting in parent shrimps; fifthly, controlling water quality; sixthly, performing polyculture of fishes; seventhly, putting in feed; and eighthly, performing culture management. The ecological healthy culture method of Palaemon carinicauda is simple in process and low cost. Water pollution caused by excess feed residue or excrement in the culture process of Palaemon carinicauda is reduced. Medicine residue in aquatics and waters due to massively using fishery medicines to prevent and control fish diseases is reduced. The ecological healthy culture method of Palaemon carinicauda is widely applicable.

The invention discloses a microsatellite marker combination for identifying the family of palaemon carinicauda and an application method, and belongs to the field of molecular-assisted breeding in genetic breeding. The method comprises the following steps: (1) screening microsatellite loci from a genome, designing primers, and selecting eight microsatellites with high number of alleles and high polymorphism by amplification; (2) obtaining microsatellite data information by microsatellite typing, thereby realizing the identification of candidate maternal and progeny. In the invention, eight microsatellite loci with high polymorphism of the palaemon carinicauda are utilized to carry out family identification, the efficiency is high, the results are accurate and reliable, the problem of difficulty in pedigree recording in the current palaemon carinicauda breeding is solved, and the breeding effect of improved varieties can be effectively improved.

The invention provides a method for harvesting and temporarily culturing sepia esculenta in a cuttlefish-shrimp polyculture pond. The method comprises steps: selecting a common ground cage for capturing prawns and a grading catching ground cage used for a prawn-crab polyculture pond; using the grading catching ground cage to catch prawns, and then using the common ground cage to capture cuttlefishes; after the cuttlefishes are caught, oxygenating and transporting the cuttlefishes with water; preparing a buffer pond and a temporary culturing pond; putting the cuttlefishes transported with water in the buffer pond to adapt for 2-3 hours, and transferring the cuttlefishes with water to the temporary culturing pond to temporarily culture; in temporary culturing, covering a blackout cloth on the temporary culturing pond, in the first week of temporary culturing, feeding fresh and alive crablats, in the second week, feeding fresh and alive palaemon carinicauda, after two weeks, feeding iced fresh small trashes; after 20-30 days of temporary culturing, using the sepia esculenta for live selling. The survival rate of the sepia esculenta after temporary culturing can reach higher than 90%. The method completely realizes live selling of the sepia esculenta, and improves economic benefits of the sepia esculenta cultivation.

The invention provides primers for detecting a SNP marker at the EC16 locus of palaemon carinicauda and a method for detecting the SNP marker at the EC16 locus of palaemon carinicauda. The method comprises extracting genomic DNA of the individual within different geographical groups or palaemon carinicauda groups, designing specific primers at both ends of the sequence through the core sequence containing the EC16 SNP marker in a palaemon carinicauda transcriptome library, carrying out PCR amplification on the genomic DNA within different geographical groups or palaemon carinicauda groups, detecting the PCR product, carrying out restriction endonuclease digestion on the PCR product, analyzing the bands, determining the genotype of each individual and acquiring the genetic polymorphism mapof palaemon carinicauda. The primers are mainly used for genetic marking, genealogical identification and genetic map construction of the palaemon carinicauda group.

The invention provides an indoor overwintering method for a palaemon carinicauda parent. The indoor overwintering method for the palaemon carinicauda parent comprises the following steps of selecting and processing a parent overwintering pond, controlling the parent ratio, density and water temperature, and performing the overwintering period feeding management technology. The indoor overwintering method is an overwintering method suitable for the palaemon carinicauda biology, behavioristics and ecology. According to the method, the overwintering survival rate of the palaemon carinicauda can be improved to a larger extent, the overwintering survival rate can be above 80%, and the expenditure can be saved by 30%. According to the indoor overwintering method disclosed by the invention, diseases can be prevented from spreading, the coal use amount is reduced, energy saving and emission reduction can be realized, the overwintering survival rate and the economic benefit are improved, and the technical guarantee is provided for the breed conservation of the superior clones of the palaemon carinicauda. The indoor overwintering method is favorable for accelerating the healthy and sustainable development of the palaemon carinicauda breeding industry in China.

The invention discloses an annular continuous multi-batch artificial culture method for palaemon carinicauda, belonging to the technical field of aquaculture. The method comprises the steps of selection and indoor wintering of parent shrimps, wintering incubation and parent shrimp rearing, pond stocking of incubation parent shrimps, first-batch culture harvest, parent selection and remaining, second-batch culture harvest or simultaneous parent selection and remaining and third-batch culture. Through indoor culture of parent palaemon carinicauda, incubation parent shrimps are obtained in advance. By reasonably arranging the parent shrimp stocking and commodity shrimp harvest time, annular multi-batch culture is realized, and the utilization ratio and economic benefit of a culture pond are increased. Two-batch culture is realized annually in the provinces of Shandong, Jiangsu and Zhejiang in China, and three-batch culture can be realized in regions south of Guangdong province in China.

The invention relates to an ecological cultivating method for initial feed of sebastiscus marmoratus larva. The method is characterized in that a cement or film side slope is arranged in a feed cultivating pool; the non-leaking muddy or sandy sediment is arranged as the bottom sediment; the water depth is 1-2m; independent water feeding / draining facility and aerator are arranged. The method comprises the following steps: sterilizing the feed cultivating pool with water containing 20ppm bleaching powder in the period from each December to the January in next year; after 2 days, dryly disinfecting the pool with 150kg / mu quick lime; exposing for one week and then feeding water; covering a 60-mesh silk gauze at a water inlet, wherein the water resource is natural river water and the salinity is 1.2-2.0; collecting copepods from the other pond and inoculating into the feed cultivating pool, after water is fed into the feed cultivating pool for one week and water in the pool becomes yellow green; after inoculating the copepods, stocking palaemon carinicauda and 2-year old coilia ectenes and feeding prawn mixed feed for one time per day; collecting the small zooplankters including copepods nauplius, small anhistozoa, rotifer, and the like, as the initial feed of sebastiscus marmoratus larva, when the water temperature is 15 DEG C or above.

The invention discloses a microsatellite marker primer and method for authenticating an inbred family of palaemon carinicauda, and belongs to the field of molecular biology. Microsatellite marker names are ES034 and ES096. The invention further provides a method for authenticating the inbred family of the palaemon carinicauda by using the primer. According to the microsatellite marker primer and the method, the genetic relationship among individuals in a palaemon carinicauda group is authenticated by using two pairs of the microsatellite primers to judge the inbreeding level among the individuals in the group; when all the individuals have monomorphism segments at 146bp and 263bp, the authenticated group meets the requirement of inbred cultivation. The method supplies a basis for judgment of an inbred line.

The present invention provides a kind of earthen pond ecological seedling cultivation and cultivation method of white prawn, comprising the following steps: (1) selection of parent shrimp, (2) overwintering indoors, (3) intensive cultivation, (4) pond arrangement and cultivation of parent shrimp, (5) Seed cultivation, (6) Shrimp seed out of pond, (7) Breeding management, (8) Breeding harvest. The present invention selects and breeds broodstock artificially, and adopts breeding pond to simulate the marine natural ecological environment condition, can improve seed production 20-30%, culture survival rate also improves 10-15%, solves the ridge tail existing in the prior art The problem of low yield and poor quality of white shrimp wild seedling cultivation.

The invention provides a method for improving the survival rate of coilia ectenes parents during an artificial breeding period. The method is characterized by comprising the following steps of adopting artificially-bred offspring coilia ectenes adult fish which is more than two years old as reserve parents of artificial breeding; in the cultivating process of the reserve parents, pulling a net and turning over a tank for 2-3 times; before the artificial breeding, pulling the net of the reserve parents and training for 2-3 times; selecting the coilia ectenes adult fish with a completely-developed gonad as parents; conveying the selected parents by using a light-shielding dark bucket; slightly operating with water and naturally fertilizing, wherein the dosage of an oxytocic hormone is not more than 30 micrograms per kilogram and the normal saline amount per fish is not more than 0.5ml, a hasten parturition tank is circular or square; shielding and regulating; continuously micro-inflating; after the parents lay eggs, carrying out postpartum intensified cultivation; gradually adding seawater to adjust the salinity to 5-10, wherein the released density is 20-40 per tank; continuously inflating and controlling the water temperature to 22-25 DEG C; feeding opossum shrimps and palaemon carinicauda for two times per day; determining the feeding amount of the next time according to the food ration; and sucking the bottom for one time per day to remove dead fish and shrimps in time.