Microsatellite marker combination for identifying family of palaemon carinicauda and application

A technology of microsatellite marking and white shrimp, which is applied in the field of microsatellite marking combination and application to achieve the effects of short cycle, high efficiency, accurate and reliable results

Active Publication Date: 2019-02-12
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the application of microsatellite markers in the family identification of the white shrimp

Method used

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  • Microsatellite marker combination for identifying family of palaemon carinicauda and application
  • Microsatellite marker combination for identifying family of palaemon carinicauda and application
  • Microsatellite marker combination for identifying family of palaemon carinicauda and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The parentage analysis of 180 offspring and 20 candidate parents of 6 different families (F1, F2, F3, F4, H1, H2) was carried out using the above-mentioned fast and efficient method for identification of white shrimp families. These 6 families included Half-sib families with closer kinship and families with more distant kinship, H1 and H2 are maternal half-sib families, and the real mother is found from 20 candidate parents. families and their identification accuracy in the presence of parental interference.

[0046] Specific steps are as follows:

[0047] 1. Extraction of DNA from candidate parents and offspring:

[0048] Use Tiangen Marine Plant Genome Extraction Kit (Tiangen) to extract candidate female parent and offspring

[0049] Generation of genomic DNA, diluted to 50ng / μL for use.

[0050] 2. PCR amplification and genotyping:

[0051] PCR amplification is carried out using the DNA of the candidate mother and offspring as templates. PCR amplification progra...

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Abstract

The invention discloses a microsatellite marker combination for identifying the family of palaemon carinicauda and an application method, and belongs to the field of molecular-assisted breeding in genetic breeding. The method comprises the following steps: (1) screening microsatellite loci from a genome, designing primers, and selecting eight microsatellites with high number of alleles and high polymorphism by amplification; (2) obtaining microsatellite data information by microsatellite typing, thereby realizing the identification of candidate maternal and progeny. In the invention, eight microsatellite loci with high polymorphism of the palaemon carinicauda are utilized to carry out family identification, the efficiency is high, the results are accurate and reliable, the problem of difficulty in pedigree recording in the current palaemon carinicauda breeding is solved, and the breeding effect of improved varieties can be effectively improved.

Description

technical field [0001] The invention belongs to the molecular assisted breeding technology of genetic breeding, in particular to the combination and application of microsatellite markers for rapid and efficient family identification of white shrimp. Background technique [0002] White ridgetail shrimp, also known as small white shrimp, velvet shrimp, and winter jasmine, belongs to Arthropoda, Crustacea, Decapod, and Longarm Shrimp. It is widely distributed in the shallow waters along the coast of the entire Chinese mainland and the west coast of the Korean Peninsula. Highest. It has the advantages of strong reproductive ability, fast growth rate and wide environmental adaptability. It is an important small economic shrimp in my country. In addition, the white prawn is tender and delicious. It can be eaten fresh or processed into dried shrimp. Because the dried shrimp is golden yellow, it is also called "golden hook dried shrimp". In recent years, with the development of co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 于洋张倩李富花张成松
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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