316 results about "Prawn" patented technology

The invention relates to the technical field of data processing, in particular to a shrimp group health condition assessment method based on deep clustering analysis, and the method comprises the steps: monitoring health parameters; determining a shrimp group dispersion grid; determining a temporary grid; identifying a non-shrimp group abnormal grid; calculating an actual health index; and adjusting the grid step length and evaluating health. According to the method, the health condition of the shrimp group can be accurately monitored and evaluated by combining physiological and behavior data such as real-time density, saturation and activeness, abnormal grids are eliminated step by step, more representative grids are screened out, evaluation deviation caused by environmental fluctuation or measurement errors is avoided, and the evaluation accuracy is improved. The dynamic monitoring of the health condition of the prawn group is realized, and the grid step length is automatically adjusted by comparing the ideal health index with the actual health index, so that the health assessment precision and reliability are improved, and the problem of inaccurate health assessment result caused by unstable environmental parameters is effectively solved.

The invention discloses a shrimp health state early warning method and system based on water quality monitoring, and aims to improve the disease prevention and control efficiency in a shrimp culture process. The method comprises the following steps: collecting historical breeding disease data and water quality change data of a target shrimp breeding water area, and constructing a water quality pathopoiesis knowledge graph; then water quality change data in a preset monitoring time period is obtained, space-time tracing is carried out by using the water quality change prediction model, and a water quality anomaly propagation path diagram is generated; formulating a water quality monitoring strategy based on the propagation path diagram, and optimizing the layout and frequency of monitoring points; and finally, monitoring the water quality in real time according to the established monitoring strategy, and realizing dynamic early warning of the health state of the prawns by combining real-time monitoring data and a water quality pathopoiesis knowledge graph. Water quality related diseases can be effectively prevented and controlled, and the stability and yield of shrimp culture are improved.

The utility model relates to the technical field of shrimp processing, in particular to a shrimp meat clamping device. The shrimp meat clamping device comprises a spiral drill bit, a rotating mechanism and a sliding mechanism; the rotating mechanism is connected with the sliding mechanism, and the sliding mechanism is configured to be capable of driving the rotating mechanism to move in the first linear direction to get close to or away from shrimp bodies; a rotating shaft of the rotating mechanism is arranged in the first linear direction. The spiral drill bit is arranged on a rotating shaft of the rotating mechanism, and the central axis of the spiral drill bit is located in the extending direction of the rotating shaft. In this way, the prawn body clamping operation can be efficiently and rapidly completed.

The invention relates to a shrimp feeding behavior prediction and automatic feeding method based on machine vision, which comprises the following steps: S1, identifying and analyzing the behaviors of shrimps: identifying the dynamic and overall behaviors of the steps of the shrimps through feature labeling and model training of the steps of the shrimps, and judging the resting, swimming and foraging behavior states of the shrimps; s2, identifying and analyzing the behavior state of the shrimps through the body labeling features, and combining with identification of residual feed in water to adjust a feeding strategy, reduce waste and reduce water treatment load; s3, the computing power center obtains real-time image frames of the underwater high-definition camera, after computing power analysis is applied, signals are sent to the feeder control center, feedback information is received, and signal linkage is achieved; and S4, video real-time monitoring and breeding data real-time display. According to the method, the training model for accurately identifying the prawn foraging behavior can be realized, the method can adapt to the complex and changeable underwater environment of the circulating water culture pond, the technical robustness is high, accurate identification can be realized, and the intelligent degree is high.

The invention relates to the technical field of prawn culture, in particular to a high-density culture optimization method for penaeus monodon based on multi-source culture environment analysis. A scale evaluation system of different preset cultivation environment condition combinations is built based on high-density penaeus monodon cultivation to evaluate the trend radiation diagram, whether the current cultivation density scale of the penaeus monodon is reasonable or not is analyzed through thermal polymerization after evaluation, and a multi-source cultivation environment analysis result is obtained; if one or more high-density abnormal breeding areas exist in a multi-source breeding environment analysis result, a simplex table model is constructed with the maximum bearing force as a constraint and the expected breeding density coefficient as a target function, and the optimal solution of the simplex table model is solved to determine the optimal breeding density scale of the high-density abnormal breeding areas. According to the method, the multi-source culture environment data can be fused to reasonably and scientifically analyze the high-density culture of the penaeus monodon and make an accurate and optimized culture strategy, so that the yield of the penaeus monodon per unit area is increased, and the low survival rate of the high-density culture is improved.

The invention discloses a new prawn culture optimization method and system based on water quality characteristic analysis. The method comprises the following steps: firstly, acquiring water environment data and new prawn growth condition data of each sub-region of a target new prawn culture water area, and performing difference analysis to obtain new prawn culture difference data of each sub-region; then, key factors influencing the growth of the new prawns are determined, and the optimal culture parameter range of the factors is analyzed to serve as culture reference parameters; secondly, acquiring actual water environment data of a water area to be cultured, comparing the actual water environment data with the reference parameters, and calculating deviation degree data; and according to the deviation degree data, evaluating the cultivation suitability, if the suitability is lower than a preset value, performing water environment regulation and control on the to-be-cultivated water area by optimizing the ecological conditions of the protists, and formulating a cultivation optimization scheme. According to the method, by accurately analyzing and adjusting the water quality and the growth conditions, the new prawn culture environment is optimized, and the culture efficiency and the prawn growth quality are improved.

The invention relates to a seawater multi-nutrient mixed culture device for shrimps, shells and gallbladders and a using method thereof, the mixed culture device comprises a culture box and net cages, floating plates are fixedly connected to the net cages, and symmetrically arranged adsorption plates are slidably connected to the interior of the culture box. Sea urchins are cultured in the net cage of the mixed culture device, and prawns and mussels are cultured in the culture box. By adopting the mixed culture device for mixed culture of shrimps, shells and gallbladders, on one hand, deep cleaning of the inner wall of the culture box is facilitated, the time consumed for cleaning the inner wall of the culture box can be shortened, and the convenience of cleaning the culture box is improved; on the other hand, the total survival rate of species bred in a mixed mode is 100%, the total net yield value of unit water is larger than or equal to 6.4 g / L, the yield of unit area is increased, and the environmental risk possibly caused by single breeding is reduced.

The invention discloses a net cage device for enhancement and releasing marine temporary rearing of Chinese prawns, the structure of the net cage device comprises a net cage, a dismounting plate, a rope and a floating ball, the dismounting plate is arranged at the left end of the net cage, the outer surface of the net cage is connected with one end of the rope, and the floating ball is fixed at the other end of the rope. Placing frames are installed on the two sides of the interior of a clamping frame in a sliding mode, then the clamping frame extends into the net cage through a dismounting and mounting plate, prawns are located in the net cage, flowing of water can be ensured through a breathable net and a filter screen plate, meanwhile, sundries are effectively blocked, and the prawns in the placing frames are prevented from being affected by the sundries; through cooperation of the water inlet and the slow flow pipe, seawater forms a slow flow state when flowing into the net cage from the outer side of the water inlet, normal flowing of the seawater in the net cage is ensured, and meanwhile the situation that prawns in the containing frame are injured or dead and flushed out due to impact of seawater with too large flow and too high flow speed is avoided.

The invention relates to application of a fermented prawn shell feed composite material in sipunculus nudus culture, and belongs to the field of feed. Prawn shells are processed by utilizing local rich prawns in Zhanjiang, and a grain-saving animal protein source, namely, shrimp shell protein, is developed through an upgrading fermentation technology; and then forming the special feed for sipunculus nudus culture by matching with algae and the like. And a special and serialized high-efficiency precise fermented feed product system for aquaculture animals is constructed. Recycling of agricultural wastes in the same area is realized; the indoor high-density breeding of the Zhanjiang sandworm is realized.

The present invention discloses a method and application of preparing a freshness indicator label by electrostatic spinning, which is obtained by the following steps: S1: adding bayberry anthocyanin to a matrix solution to obtain a spinning solution; S2: after ultrasonic filtration of the matrix solution, taking part of the solution and putting it into a 10ml syringe, fixing it on a propulsion pump, spinning using a high-voltage electrostatic spinning device under a specific voltage, receiving distance, flow rate, temperature and humidity to prepare a nanofiber membrane; S3: balancing the prepared nanofiber membrane for 48 hours, and cutting it into a specific size to prepare a freshness indicator label. The freshness indicator label prepared by the method of the present invention has a large specific surface area and porosity, and is sensitive to pH response. The bayberry anthocyanin freshness indicator label based on pullulan / polyvinyl alcohol has great application potential in the freshness monitoring and intelligent packaging of white shrimp.

The invention discloses a group of shark nano antibodies targeting infectious subcutaneous and hematopoietic necrosis viruses of prawns and a preparation method of the shark nano antibodies. The amino acid sequence of the nano antibodies is any one amino acid sequence selected from SEQ ID NO.8-14, and the nano antibodies can be used for detecting cp, nsp1 and nsp2 antigen proteins of the infectious subcutaneous and hematopoietic necrosis viruses. The preparation or screening method of the nano antibody comprises the following steps: A, expression and purification of cp, nsp1 and nsp2 antigen proteins; b, constructing a shark natural library and / or a shark immune library; c, assisting in phage rescue, and purifying to obtain a phage library; d, panning a phage library of anti-cp, anti-nsp1 and anti-nsp2 antigen proteins; and E, carrying out phage-ELISA identification and sequencing, and carrying out sequence comparison on the cp nano antibody, the nsp1 nano antibody and the nsp2 nano antibody with different CDR3 region sequences. The nano antibody provided by the invention can specifically recognize infectious subcutaneous and hematopoietic necrosis viruses, can detect whether aquaculture animals and aquaculture feeds in aquaculture water are polluted by viruses or not, and realizes rapid detection.

The present application belongs to the field of aquaculture technology, and mainly relates to a traditional Chinese medicine composition for preventing and treating acute hepatopancreas necrosis disease of prawn, a preparation method and application. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 28-32 parts of Radix Sanguisorbae, 28-32 parts of Radix Scutellariae, 18-22 parts of Phellodendri Cortex and 8-12 parts of Lonicerae Japonicae Flos. The traditional Chinese medicine composition can significantly inhibit the activity of the main pathogen of the disease, i.e. Vibrio parahaemolyticus, improve the immune function and disease resistance of prawn, and further repair the hepatopancreas and intestinal tissues of the diseased prawn, thereby achieving the effect of effectively preventing and treating the acute hepatopancreas necrosis disease of prawn.

The invention discloses a method for culturing seawater rotifers by using prawn shell leavening, which comprises the following steps: S1, cleaning prawn shells to obtain a fermentation substrate; s2, dynamically optimizing fermentation conditions by adopting a multi-agent collaborative reinforcement learning algorithm; s3, predicting the optimal completion time of fermentation by adopting a mixed graph convolutional neural network and an adaptive genetic algorithm, and providing dilution suggestions through a spectral analysis technology; s4, dynamically calculating the dilution ratio, and mixing the fermentation liquor with seawater; s5, pouring the diluent into a rotifer culture pond, predicting the health state of the rotifer by adopting a Vision Transform model, and automatically adjusting a nutrition formula or environmental conditions; s6, monitoring the density of the rotifers in real time through a YOLOv5 target detection model and a camera, and keeping the rotifers within a preset density range; and S7, performing intelligent management on waste treatment based on a multi-objective evolutionary algorithm of non-dominated sorting. According to the method, a multi-agent collaborative algorithm and a deep learning technology are adopted, and intelligent culture of seawater rotifers and optimized treatment of wastes are realized.

The invention relates to the technical field of aquatic product freezing processing, and discloses a prawn freezing process dynamic regulation and control method and system, and the method comprises the steps: extracting a damage feature vector through a lightweight instance segmentation network; generating a lightweight knowledge graph based on knowledge distillation; the damage features are converted into symbolic representation, and neural symbolic reasoning is carried out; calculating a quality degradation risk index and generating a differential control parameter; and collecting feedback data and updating the weight of the knowledge graph. According to the method, the technical problems of low response speed and poor interpretability in the freezing process of the damaged prawns are solved, and millisecond-level real-time decision and accurate control are realized.

The invention discloses a multiplex fluorescent quantitative PCR (polymerase chain reaction) detection kit for prawn culture pathogenic bacteria, which is characterized in that the kit contains primers and probes for detecting the prawn culture pathogenic bacteria, wherein the primers and the probes are respectively used for detecting TDH-related hemolsin genes of vibrio parahaemolyticus, ORF107 genes of white spot syndrome viruses, small subunit ribosomal genes of shrimp enterocytozoon hepatopenaei, 37 kDa coat protein genes of infectious subcutaneous and hematopoietic necrosis viruses, DNA (deoxyribonucleic acid) primer genes of full-eye iridovirus 1 and PirA [pi] genes of pathogens of bacterial prawns acute hepatopancreas necrosis; the method has the advantage that the detection of eight prawn culture pathogens can be realized at the same time. The method comprises the following steps of: obtaining a tcdB gene of a high-pathogenicity vibrio and a hemolsin gene of a vibrio harveyi;

The invention discloses a primer probe composition for detecting prawn disease related pathogens, a kit and application of the primer probe composition. The primer probe composition comprises a first primer probe group used for detecting vibrio parahaemolyticus, a second primer probe group used for detecting a tetrapod iridovirus 1, a third primer probe group used for detecting vibrio vitriocultatus and a fourth primer probe group used for detecting enterocytozoon hepatopenaei. The primer probe composition has the characteristics of high sensitivity, good repeatability and strong specificity, and can be used for specifically detecting vibrio parahaemolyticus, tetrapod iridovirus 1, vibrio vitrioculi and enterocytozoon hepatopenaei.

The method comprises two steps of large-scale breeding of temperature-resistant groups and temperature tolerance comparison of individuals, the large-scale breeding of the temperature-resistant groups comprises group temporary rearing, temperature setting, temperature tolerance testing and vigor testing, and the temperature tolerance comparison of the individuals comprises group temporary rearing, temperature setting, vigor testing, temperature stress and vigor testing. The method efficiently solves the problem of batch breeding of temperature-resistant groups in the production process of the procambarus clarkia and the problem of rapid evaluation of individual temperature-resistant character differences in a living body at low cost in the experiment process, reduces irreversible damage of traditional heat-resistant index death rate evaluation on prawn bodies, and improves the evaluation accuracy of the procambarus clarkia temperature-resistant character differences of the procambarus clarkia temperature-resistant character differences of the procambarus clarkia. The method greatly shortens the complexity and time consumption of temperature-resistant heart rate inflection point measurement, is low in cost, large in scale and high in speed, combines ecological adaptation knowledge of physiology, behavioristics and the like of crustacean, comprehensively applies multiple indexes to temperature-resistant performance evaluation, makes up for the deficiency of single-index breeding, and better conforms to natural conditions and production practice.

The invention discloses a prawn larva counting method based on improved YOLOv5. The prawn larva counting method comprises the following steps: S1, obtaining a three-dimensional depth field; s2, inputting image frames of the enhanced continuous video stream image into the improved YOLOv5 detection model, and outputting an initial candidate bounding box set; s3, performing spatial correlation on the initial candidate bounding box set and the three-dimensional depth field to generate a target three-dimensional bounding box set; s4, constructing a fitness function based on the target three-dimensional bounding box set, and outputting a current environment optimal hyper-parameter set; s5, outputting a prawn larva target detection result set subjected to three-dimensional space fusion optimization; and S6, performing intra-frame counting operation based on the prawn larva target detection result set, and calculating a prawn larva density value per unit volume to obtain a prawn larva real-time quantity statistical result. According to the invention, automatic monitoring and fine management of prawn culture links are effectively supported.

The invention relates to a new prawn breeding data analysis and recommendation method, a new prawn breeding data analysis and recommendation system and a medium, and belongs to the technical field of new prawn breeding. According to the method, survival rate data of a plurality of new prawn types in a current culture area are obtained, secondary selection is carried out on the survival rate data of the plurality of new prawn types in the current culture area, a final new prawn type is obtained, and finally, seedling culture arrangement is carried out on the current culture area based on the final new prawn type. And monitoring the breeding data of the new prawns in the current breeding area. According to the method, the optimal new prawn variety is recommended according to the survival rate data of each new prawn variety under each environment characteristic data, so that the recommended new prawn variety is more suitable for culture, the culture success rate is improved, and the economic benefits of farmers are further improved.

A head-removed shrimp posture detection method based on machine vision comprises the following steps: carrying out three-channel separation on a head-removed shrimp image in an HSV space, and selecting an optimal channel according to a foreground and background difference to obtain a head-removed shrimp binary image; obtaining three distance transformation heat maps of the binarized image of the head-removed shrimp, and selecting an optimal heat map according to the extraction matching degree of the central area of the shrimp head to obtain a central area image of the shrimp head; performing corner detection on the prawn head center area image, and fitting corner coordinates through a straight line to obtain a prawn head and prawn back orientation datum line; comparing the distances between the two wide sides of the minimum enclosing rectangle of the binarized image of the head-removed shrimp and the two end points of the shrimp head orientation datum line to obtain a shrimp head orientation vector; comparing the distance between the long side, tightly attached to the shrimp back, in the minimum bounding rectangle of the binarized image of the head-removed shrimp and the two end points of the shrimp back orientation datum line to obtain a shrimp back orientation vector; and the orientation vectors of the shrimp head and the shrimp back represent the attitude information of the head-removed shrimp. The method can accurately and stably detect the postures of the head-removed shrimps at low cost, and belongs to the shrimp posture image processing technology.

The invention provides heat-resistant bacillus pumilus P2-7 and application thereof in disease prevention and control of prawn culture, and belongs to the technical field of bioengineering. The heat-resistant bacillus pumilus P2-7 provided by the invention is preserved in Guangdong Microbial Culture Collection Center on November 4, 2024, and the preservation number of the heat-resistant bacillus pumilus P2-7 is GDMCC NO. 65408. The strain is different from the traditional antibacterial bacillus in antagonism mode, cannot secrete metabolites with antibacterial activity, plays a role in a phagocytosis mode, can remarkably remove / inhibit pathogenic bacteria in water, can effectively prevent and control shewanella disease of prawns, and has a good application prospect. The composition also has a good effect of simultaneously preventing septicemia, acute hepatopancreas necrosis, white spot syndrome and infectious subcutaneous and hematopoietic necrosis in prawn culture production.

The invention provides acinetobacter for culturing prawns in saline-alkali water and application of the acinetobacter. The preservation number of the provided acinetobacter is CGMCC (China General Microbiological Culture Collection Center) No.36066. The acinetobacter soilis strain provided by the invention has remarkable comprehensive application advantages, can still keep higher growth activity and metabolic capability in a low-temperature environment, and continuously plays a water body purification function. Ammonia nitrogen and nitrite nitrogen in a water body can be efficiently degraded, the environment of the aquatic water body is remarkably improved by promoting the nitrogen circulation process, and harm caused by nitrogen pollution is reduced. In addition, the strain can effectively inhibit and prevent reproduction of pathogenic vibrios and reduce the occurrence risk of bacterial diseases of aquatic animals. The strain is high in safety, does not cause poison or stress reaction to bred animals even under the condition of high-concentration use, and has good ecological safety and environmental friendliness.

The invention belongs to the technical field of pathogenic microorganism screening, and particularly relates to vibrio parahaemolyticus capable of causing prawn vibrio vitreovibriosis and application of the vibrio parahaemolyticus, and the pathogenic bacteria can be used for preparing inactivated vaccines or used as the pathogenic bacteria to screen medicine components with the effect of preventing and treating the prawn vibrio vitreovibriosis or a culture method. The preservation number of the vibrio parahaemolyticus provided by the invention is CGMCC (China General Microbiological Culture Collection Center) The pathogenicity of the vibrio parahaemolyticus with high pathogenicity and strong lethality is about 1000 times stronger than that of the vibrio parahaemolyticus which is collected by the world animal hygiene organization and causes acute hepatopancreatic necrosis, and the strain is popular in main prawn culture areas and causes serious harm to the prawn fry culture industry in China. The vibrio parahaemolyticus provided by the invention can be used as a positive control for preparing a diagnosis product for the prawn vibrio vitreovirus disease; or preparing an immune vaccine after inactivation.

The sorting systems and methods described herein provide for sorting of prawns. The sorting system may be combined with a delivery system. The sorting system employs rotating slide pairs and optional guide chutes to deliver shrimps to selected delivery locations.

The utility model relates to an auxiliary monitoring device, and belongs to the field of shrimp culture. The auxiliary monitoring device comprises a floating base, a lifting mechanism and a shrimp fishnet, the lifting mechanism is arranged on the floating base, the fishnet is connected with the lifting mechanism, and the lifting mechanism drives the fishnet to reciprocate in the vertical direction; a controller and a camera module are arranged on the floating base, the lifting mechanism and the camera module are electrically connected with the controller, and a lens of the camera module faces the fishnet. According to the scheme provided by the utility model, long-term monitoring of prawn growth, feeding conditions and health states is realized in a mode of replacing manual work with machinery, and the prawn monitoring device has the characteristics of easiness in operation and convenience in use.

The invention provides a reagent for detecting the proportion of vibrio in prawn seeds or prawn culture water in total bacteria. The reagent comprises a vibrio detection primer and probe, a bacteria detection primer and probe, Taq DNA polymerase, a dNTP mixture, UNG enzyme, a freeze-drying protective additive and a PCR buffer solution. According to the method, the specific DNA of the vibrio and the bacteria in the sample is quantitatively detected by utilizing the fluorescent PCR technology, the proportion of the vibrio in the total bacteria is obtained through the obtained quantitative detection data, the proportion of the vibrio in the total bacteria in shrimp seeds and shrimp culture water can be obtained in a short time, and guidance is provided for shrimp breeding and culture.

The invention belongs to the technical field of disease control and aquaculture, relates to specific therapeutic activity of metformin hydrochloride, and particularly relates to application of metformin hydrochloride in preparation of a medicine for resisting white spot syndrome. Experiments prove that the metformin hydrochloride can endow prawns with certain white spot syndrome virus resistance by inducing expression up-regulation of key members Ago2 and Dicer2 genes in a prawn RNAi (Ribonucleic Acid Interference) antiviral mechanism and antiviral effect factors AlfB1 and Dapper genes.

The invention discloses a prawn health status assessment method based on multi-source information fusion, and relates to the technical field of data processing. Comprising the following steps: obtaining prawn images in different growth stages and preprocessing the images, generating training images by using artificial marks, and extracting characteristics such as the contour length, the area and the shell contrast of prawns; based on the training image, establishing a target identification and feature extraction model, after training, identifying prawns in the to-be-monitored culture environment and extracting behavior feature parameters of the prawns; calculating a behavior evaluation influence coefficient and a growth evaluation influence coefficient, and obtaining a water source characteristic parameter to calculate a disease incidence influence coefficient; and finally, comprehensively generating a health state evaluation index, comparing the health state evaluation index with a health judgment threshold value, and outputting a health state judgment result, thereby realizing effective evaluation, real-time monitoring and early warning of the prawn health state to help a farmer to take measures in time and prevent diseases, and improving the survival rate and economic benefit of prawn culture.

The utility model discloses a blowdown device for prawn culture. The blowdown device is arranged on a prawn culture pond. The sewage discharging device comprises a sewage suction pump, a filter screen arranged on the sewage suction pump in a covering mode, a supporting frame, two sets of rotating discs horizontally arranged on the two corresponding sides of the sewage suction pump, three sets of sweeping arms fixedly installed on the corresponding rotating discs, a rotation driving structure acting on the rotating discs and a water quality sensor fixedly installed on the sewage suction pump. And the controller is arranged on the support frame. The rotating driving structure comprises a rotating motor, a driving shaft and a driven rod which are vertically and rotatably mounted on the supporting frame in a penetrating manner, two groups of gears, and a crawler belt which is mounted between the two groups of gears in a meshing manner. The device is directly placed in the prawn culture pond for use, can quickly and efficiently finish the pollution discharge of the culture pond under the condition of reducing the transformation of the culture pond as much as possible, can prevent the pollution discharge equipment from being blocked, does not need to clean the blockage by manpower, and reduces the labor investment.

The invention discloses a shrimp and crab feeding monitoring method and system based on a passive acoustic technology, and belongs to the technical field of aquaculture. According to the method, breeding acoustic signals are collected, shrimp and crab feeding acoustic signals are extracted through preprocessing, and then a feeding time-frequency distribution diagram is obtained through time-frequency analysis. A standard time-frequency distribution map library containing different feeding grades is constructed, an image hash technology is used for processing actual feeding signals and signals in the standard map library, fingerprints are obtained and matched, and therefore the feeding intensity of shrimps and crabs is accurately determined. According to the invention, the feeding activity of prawns and crabs can be accurately and effectively monitored, and the feeding strategy can be automatically adjusted according to the feeding intensity.