22results about How to "Avoid death" patented technology

The invention discloses a construction method of a conditional knock-down dynactin animal model of astrocytes and an application of the animal model of the conditional knock-down dynactin animal model of the astrocytes. According to the method, a Dctn1LoxP gene knock-in mouse is hybridized with an Aldh1l1-Cre / ERT2 transgenic mouse, a target genotype mouse is obtained through three rounds of breeding, 100mg / kg Tamoxifen is continuously injected into the intraperitoneal cavity of the 2-month-old mouse for 5 days, and specific knock-down of dynactin in brain and spinal astrocytes is realized, including knockout of p150Glue and reduction of DCTN4, p50 and Arp1alpha protein levels. The model has the advantages of being high in specificity, permanent in intervention aging and capable of covering multiple life stages, the defects of a traditional model are overcome, the model can be used for researching the influence of dynactin on astrocyte neurobiological functions, a reliable tool is provided for screening related targets of nervous system diseases, and the model has important application value.

The utility model discloses an ecological restoration device for sand vegetation. The ecological restoration device comprises a frame; the supporting rods are arranged on the frame in a circumferential array and are in running fit with the frame; ground nails are fixed to the bottom end of the frame in a circumferential array mode, and drainage holes are formed in the bottom ends of the ground nails; the flow guide pipes are circumferentially arrayed on the side wall of the frame and are communicated with the drainage holes; and the water collecting assembly at least comprises conical blocks which are circumferentially arrayed on the side wall of the frame. According to the sand vegetation ecological restoration device, through the arrangement of the frame, the supporting rods, the ground nails, the drainage holes, the flow guide pipes and the conical blocks, water vapor in air can be condensed into water drops by means of the characteristic that the temperature difference between day and night in desert is large after saplings are fixed in sandy soil, and the water drops are collected and supplied to the roots of the saplings, so that the saplings are protected from being damaged. The saplings can absorb more water sources, large-area death of the saplings which are just planted due to water shortage is avoided, the survival rate of the saplings in the desert is increased, and the restoration speed of an ecological system is higher.

This invention relates to animal models and their applications, specifically to a method for constructing a spontaneous mouse cerebral edema model and its application. This invention utilizes PEN2... fl / + Mice and PEN2 fl / + Mice were mated to obtain PEN2. fl / fl Homozygous mice; then the obtained PEN2 fl / fl Mice and hGFAP Cre / + Mice were mated to obtain PEN2. fl / + hGFAP Cre / + Mice; the final PEN2 obtained fl / + hGFAP Cre / + Mice and PEN2 fl / fl Mice were mated to obtain PEN2. fl / fl hGFAP Cre / + The mouse model is a spontaneous mouse cerebral edema model. In this model, PEN2 protein levels are significantly downregulated, and the mouse exhibits a severe cerebral edema phenotype. This model can be used to establish a cerebral edema drug screening platform. It can also be used for research on cerebral edema-related diseases, such as the pathogenesis of cerebral edema caused by infection, glioma, and traumatic brain injury, as well as for the development of technologies and products for the diagnosis and treatment of related diseases.

The application discloses a water-saving irrigation device capable of coping with crops with different densities and a method thereof, and belongs to the technical field of irrigation devices. The water-saving irrigation device comprises a U-shaped pipe, the upper end of the U-shaped pipe is provided with a continuous water supply assembly for ensuring continuous water supply to plants, the continuous water supply assembly comprises a water spraying irrigation device and a sealing blockage alarm device, the U-shaped pipe is connected with the water spraying irrigation device, and the water spraying irrigation irrigation device is connected with the sealing blockage alarm device. An irrigation adjusting device for adjusting plant irrigation is arranged on the U-shaped pipe. A fertilizer stirring and conveying device for providing the device with fertilizer is arranged on the irrigation adjusting device. A feeding device for conveying fertilizer into the device is arranged on the fertilizer stirring and conveying device. In the above manner, when the device is blocked, the standby device is started to supply water to the plants, so that the death of the plants caused by uneven irrigation is avoided, and when the standby device is started, an alarm is sent in a timely manner to remind the staff to replace the device.

This utility model relates to the field of rat smoking technology and discloses a rat smoking device, including a smoking chamber, a three-way valve, a smoking chamber, and an extraction and delivery component. The three ends of the three-way valve are detachably connected to a first hose, a second hose, and a third hose, respectively. The first hose is detachably connected to the smoking chamber. The smoking chamber is detachably connected to the third hose, and the smoking chamber can be used for burning cigarettes. The extraction and delivery component is detachably connected to the second hose and is used to draw the smoke from the burning cigarette in the smoking chamber through the third hose, the three-way valve, and the second hose. This utility model proposes a rat smoking device that generates smoke through the smoking chamber, which is then delivered to the smoking chamber via hoses and a three-way valve to smoke the rats, simulating the smoking process and avoiding experimental errors. Simultaneously, fresh air is introduced and smoke is expelled, simulating a natural smoking environment, diluting the smoke concentration, ensuring the survival safety of the rats, extending the smoking time, and obtaining more data on the chronic development of COPD.

The invention discloses a finned radiator of a bidirectional protection type transformer, and belongs to the technical field of oil-immersed transformer components. Which comprises an oil inlet pipe connected with an oil outlet of the transformer, an oil return pipe connected with an oil inlet of the transformer and a group of radiating fins, and is characterized in that a protective cover rear fixing mechanism is arranged on the rear side of the oil inlet pipe, and a protective cover front fixing mechanism is arranged on the front side of the oil return pipe; a protective cover is fixed between the protective cover rear fixing mechanism and the protective cover front fixing mechanism, the left side face of the protective cover forms an opening, the upper side face, the lower side face, the front side face, the rear side face and the right side face of the protective cover form a hollow net-shaped structure, and the protective cover forms a protective cover cavity used for containing the finned radiator. The finned radiator has the advantages that birds resting on a foot can be prevented from being scalded by high-temperature radiating fins and even dying, and the surfaces of the radiating fins of the finned radiator can be prevented from being contaminated by sundries such as deadwood, residual leaves and flying catkins of external trees and bird droppings; ideal staticizing requirements are embodied, and energy is saved; and manpower resources are saved.

The utility model relates to the technical field of alfalfa planting, in particular to an alfalfa cuttage sand culture device which comprises a sand bed body, a cuttage mechanism and a lifting mechanism. The sand bed body is provided with vent holes and a filter screen to guarantee ventilation of the bottom and prevent sand from leaking out; a cutting support of the cutting mechanism is in sliding connection with a sliding rail on the inner wall of the sand bed through a sliding block, the position and distance of a cutting assembly on the cutting support can be adjusted through an adjusting mechanism, and the adjusting mechanism controls the cutting operation process through a limiting bolt, a rotating cylinder and other components; a lifting frame of the lifting mechanism can horizontally slide on the sand bed body, and a lifting screw in threaded connection drives the cuttage support to ascend and descend, so that cuttage depth adjustment is achieved. The device improves the air permeability of the sand bed by accurately adjusting the cuttage position, spacing and depth of the alfalfa seedlings, provides a good growth environment for the alfalfa seedlings, effectively improves the survival rate and yield of the alfalfa seedlings, and is suitable for efficient cultivation of the alfalfa.

The utility model discloses a device for quickly removing Brucella in small piercing-sucking mouthpart or filing-sucking mouthpart insects, which belongs to the field of biotechnology, and comprises a glass bottle, a degerming liner and a bottle cap, the color of the glass bottle is blue or yellow, the degerming liner is arranged in the glass bottle, the degerming liner is made of a transparent material, and the bottle cap is arranged in the glass bottle. The bottle cap is in threaded connection with the top end of the glass bottle, and a gauze element is arranged on the bottle cap. The insect killing device is simple in structure and convenient to use, insects are attracted through the blue or yellow glass bottle, the insects are attached to the bacteria removing film of the bacteria removing inner container, bacteria removing is conducted on the insects through the bacteria removing liquid on the bacteria removing film, the activity of the insects is reduced, and the survival rate during bacteria removing is improved.

The utility model discloses a seedling pool drainage seedling protection device, include: flow guide cylinder, the bottom fixedly connected with the blocking cylinder of flow guide cylinder, the inside of blocking cylinder is provided with blocking subassembly, and blocking subassembly is used for blocking the seedling in seedling pool, a plurality of extension seat, a plurality of extension seat even fixedly connected in the outside of blocking cylinder, the middle part of each extension seat is slidably connected with the support rod, and the top of each support rod is fixedly connected with the linkage plate, and the linkage plate between fixedly connected with linkage ring, the lock plate of cooperation, the lock plate fixedly connected in the outside of flow guide cylinder, and the outside of linkage ring is equipped with locking assembly, and locking assembly is used for the positioning of support rod adjustment with the lock plate cooperation and forms, the utility model relates to aquaculture technical field, the utility model discloses through the structural cooperation of support rod, lock plate and locking assembly, can when discharging, the liquid balance of seedling pool is controlled conveniently, so that the pool bottom temperature keeps stable, avoids the death due to temperature change.

The invention belongs to the field of genetic engineering animal models, and particularly relates to a specific Foxp4 gene knockout mouse model and a construction method and application thereof. The method comprises the following steps: by utilizing a Cre-loxP system, obtaining Foxp4-fl / fl from a Foxp4-fl / fl from a Foxp4-fl / + heterozygote mouse and an Alb-Cre mouse through a mating strategy; and the liver-specific Foxp4 knockout mouse model is constructed by using the Foxp4 knockout mouse and an Albcre / + mouse. The invention finds for the first time that the Foxp4 plays an important role in maintaining the steady state function of the liver, the deficiency of the Foxp4 leads to phenotypes such as lipid accumulation in the liver, inflammatory cell infiltration, liver cell function damage, mild fibrosis and abnormal glucose and lipid metabolism, and the phenotypes are highly consistent with early symptoms and molecular characteristics of NAFLD; the Foxp4 gene has important application value in the aspects of researching the effect of the Foxp4 gene in the occurrence and development mechanism of the NAFLD and the maintenance of liver homeostasis, developing NAFLD-related therapeutic drugs and the like.

The utility model discloses an insect collecting tube, and relates to the technical field of entomology research experiment equipment, the insect collecting tube comprises a tube body, a tube cover, a male buckle assembly and a female buckle assembly, the tube body is used for accommodating insects, one end of the tube body is open, the other end of the tube body is closed, and the tube cover is used for closing the open end of the tube body; a ventilation part is further arranged on the pipe body, and the ventilation part is used for ventilation of air in the pipe body and external air; the male buckle assembly and the female buckle assembly are arranged on the two sides of the pipe bodies correspondingly so that the multiple pipe bodies can be detachably connected. The insect collecting device has good ventilation performance and is convenient to use when insects are narcotized by ethyl acetate, meanwhile, a plurality of insect collecting pipes can be connected in rows, the insect collecting device is convenient to carry during field operation, jolt is effectively reduced, and the collected insects are prevented from being damaged.

The invention relates to a construction method and application of a mouse model with spontaneous gastric precancerous lesions. The construction method comprises the following steps: constructing a Pgc-Cre gene mouse only carrying an inducible Cre gene (tamoxifen can be injected in a rich manner to induce Cre nucleation); the method comprises the following steps: constructing a Rosa26-Zeb2 gene mouse which only carries a Zeb2 gene; the method comprises the following steps: taking Cre and Zeb2 genes as raw materials, mating to obtain a mouse carrying the Cre and Zeb2 genes at the same time, then administering a drug to the mouse, inducing Cre to enter a nucleus and promoting expression of the Zeb2 genes to obtain the mouse model with the spontaneous precancerous lesion of the gastric cancer; the medicine is tamoxifen. The mouse model constructed by the invention is driven by the specific inducible Cre of the stomach main cells, is limited to the stomach main cells, avoids gene disturbance of non-target cells, is simple and convenient to mate, low in cost and short in experimental period, and can be used for screening gastric precancerous lesion drugs.

This application relates to the field of cell electroporation technology, and provides a multifunctional cell culture multiwell plate for electroporation, including a consumable cover, a consumable interlayer, and a consumable base plate. The top surface of the base plate has multiple wells for containing cell solutions. The consumable interlayer is located on top of the base plate, and a cavity for containing cell solutions is formed between the consumable interlayer and the base plate. Electrode slots are provided on the consumable interlayer at positions corresponding to both sides of each well. The consumable cover is located on top of the interlayer, and a removable electrode is provided on the cover at a position corresponding to each electrode slot. An impedance detection electrode assembly is provided on the bottom surface of the base plate at a position corresponding to each well. This application improves the practicality of the multifunctional cell culture multiwell plate.

The utility model provides an edible mushroom planting hydroponic device, including culture box, culture mechanism, one side bottom of culture box is equipped with liquid inlet and liquid outlet, the culture box both sides are symmetrically equipped with a plurality of card slot, culture mechanism is composed of mounting box and culture net, the both ends of mounting box are equipped with the card slot mutually clamped with the card slot mutually, the card slot is equipped with the card slot, the card slot is equipped with the card slot mutually clamped with the card slot, and the card slot is equipped with the card slot mutually clamped with the card slot. The cultivation net penetrates into the installation box from the bottom of the installation box and is arranged in the installation box in a sliding mode, two guide rods penetrating through the installation box are fixedly arranged at the two ends of the cultivation net, a transverse plate is installed between the two guide rods, a threaded adjusting rod is rotationally arranged in the installation box, and the threaded adjusting rod is in threaded connection with the transverse plate. The device has the advantages of avoiding rotting and death of strains, improving the cultivation efficiency and the like.

The utility model relates to a wasp attack behavior observation box for a laboratory, and belongs to the technical field of wasp behavior observation. The box body is a square box with an opening in the top, ventilation windows are arranged on the two sides of the box body, anti-escape nets are installed on the ventilation windows, door holes are formed in the bottoms of the two ends of the box body, baffle doors are hinged to the box body through torsion springs, the box body is divided into buffering grids at the two ends and an attacking grid in the middle through partition plates, and one-way transparent coated glass is installed on the top of the box body. A through hole convenient for operating the partition plate is formed in the one-way transparent coated glass; experimental bees and invasive bees can be conveniently put in through the baffle door, the buffer grid is beneficial to relieving stress response when the wasps enter a strange environment, the partition plate can be freely taken out when attack behavior measurement is started, the situation in the box can be clearly seen from the outside of the box through the one-way transparent coated glass at the top, and the situation outside the box cannot be seen from the inside of the box. In addition, light in the box is dark, interference to the wasps is avoided, and experimenters can directly observe attack behaviors of the wasps.

The application discloses a kind of agrobacterium-mediated panax ginseng alternaria gene transformation methods, comprising: (1) co-culture: after being activated and induced to cultivate, the recombinant agrobacterium containing target gene is co-cultured with panax ginseng alternaria mycelium suspension in IM culture medium;(2) screening: after co-culture, when 5-8 panax ginseng alternaria colonies are observed, the mycelium area of colony accounts for 30%-60% of the total area of IM culture medium, while the number of agrobacterium plaque is not more than 10, pour a layer of PDA solid medium containing screening antibiotic on IM culture medium to carry out double-layer screening;(3) verification.The agrobacterium-mediated panax ginseng alternaria gene transformation method of the application can effectively inhibit the excessive growth of agrobacterium and greatly improve the true positive rate, and realizes the best balance of agrobacterium infection activity and fungal tolerance, avoids the death of fungal cells due to excessive infection, or transformation failure due to insufficient infection, provides a reliable technical means for transforming panax ginseng alternaria strain and verification.

The utility model discloses an automatic cleaning machine for crayfish processing, and mainly relates to an automatic cleaning machine for crayfish processing, the automatic cleaning machine for crayfish processing comprises a water tank, a double-screw conveying cleaning device, a rotating mechanism, a suction device, a brush cleaning device and a crayfish line extraction device, the upper end of the water tank is provided with a conveyor belt, and the surface of the conveyor belt is provided with a crescent baffle plate; a plurality of sets of crescent baffles are arranged, a material distributing plate is placed at the end, away from the water tank, of the conveying belt, spring supports are arranged at the peripheral corners of the lower end face of the material distributing plate, and a vibration motor is fixedly installed on the lower end face of the material distributing plate. The device has the advantages that the rolling of the crayfish is reduced, the death rate of the crayfish is reduced, the chest and abdomen of the crayfish are directionally cleaned, and the shrimp lines of the fresh and alive crayfish are removed.

The utility model provides a kind of fry screening device for aquaculture, it is related to aquaculture technical field, including organism, the inside of the organism is provided with adjustable shaking screening mechanism, the side of the organism is provided with humidification mechanism, the other side of the organism is equipped with PLC controller, the wiring end of the PLC controller is connected with device inner wire arrangement.This utility model in, by setting adjustable shaking screening mechanism, first servo motor drives the rotation of one-way screw rod, moves the moving block under the restriction of first limit rod, further let rack drive gear and rotating rod rotate, realize that screen cylinder shakes, simultaneously, second servo motor drives the rotation of two-way screw rod, can adjust the interval of two adjusting plate and contact sensor, when moving block touches contact sensor, will feedback signal to PLC controller, control first servo motor reverses, to this flexible adjustment screen cylinder shaking range, solved the problem that existing device cannot flexibly adjust shaking range.

The application relates to a zero additional memory overhead parameter fault tolerance protection method and device, equipment and medium, and relates to the technical field of satellite remote sensing. According to an exponential redundancy coding strategy, a redundant copy of to-be-protected exponential information and corresponding check information are re-embedded and mapped to a low bit area of a mantissa field to generate reconstructed protection parameters. A two-way even check mechanism is used to determine the credibility of the to-be-protected exponential information and the redundant copy of the protection parameters, and corresponding exponential recovery logic is executed according to the determination result to obtain a target exponential domain. The low bit area of the mantissa field is zeroed or truncated to recombine a target mantissa field, the target exponential domain, a sign bit and the target mantissa field are combined to obtain recovered neural network floating point parameters. The application is compatible with an existing training process, effectively detects and corrects bit flip errors on key bits, and guarantees the numerical stability and original performance of a model during on-orbit operation without additional storage overhead.

The present invention relates to a method for providing collision risk information by simulating robot motion and an apparatus for performing the same, the method comprising the steps of: providing a script composed of a plurality of instructions corresponding to robot motion; calculating the risk degree of each motion in the robot motion; and a processing script that displays, in different colors, a first instruction corresponding to the motion of which the calculated degree of risk exceeds the reference value and a second instruction corresponding to the motion of which the degree of risk is lower than or equal to the reference value.

The utility model relates to the field of aquaculture technology discloses fry delivery box of aquaculture, including fry delivery water tank, the outer wall fixedly connected with batched fry delivery assembly of fry delivery water tank, the inner wall detachably connected with the water inlet pipe of fry delivery water tank, one end detachably connected with the tee bend of water inlet pipe, the inner wall fixedly connected with primary negative pressure pipe of tee bend, the inner wall fixedly connected with secondary negative pressure pipe of tee bend, the inside fixedly connected with tertiary negative pressure pipe of tee bend, the outer wall detachably connected with the connecting pipe of tee bend, the inside of tee bend is provided with air inlet, the outer wall of connecting pipe is connected with water suction pump. In the utility model, through the installation of oxygenation water circulation assembly, make the water in the inside through the negative pressure assembly in the tee bend inside make the oxygen in the air fully enter the fry delivery box inside, make the inside oxygen fully, greatly improved the survival rate of fry in the inside.

The utility model relates to the technical field of bee breeding, and discloses a constant temperature lamp for bee breeding, which comprises a mounting plate, the bottom of the mounting plate is fixedly connected with a connecting frame, the inside of the connecting frame is slidably connected with an adjusting frame, and the bottom of the adjusting frame is fixedly connected with a constant temperature lamp main body. An outer lampshade is fixedly connected to the outer side of the constant-temperature lamp body, a plurality of first through holes are formed in the outer lampshade, an adjusting cover is rotatably connected to the interior of the outer lampshade, a plurality of second through holes are formed in the adjusting cover, a fixing assembly is arranged on the outer sides of the second through holes, and an adjusting assembly is arranged in the connecting frame. According to the constant-temperature lamp, the motor is started to drive the rotating rod to rotate, the first gear fixed to the rotating rod rotates along with the rotating rod, the rack is driven to move up and down, then the adjusting frame fixedly connected with the rack slides in the connecting frame, and the purposes that the height of the constant-temperature lamp body is changed, and different beehive structures and bee breeding requirements are met are achieved.